Rmu_sc0000600.1_g000054

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_multiflora
Rmu_sc0000600.1
OG0000415
Physical Location & Seq
Forward (+)
270828 .. 273444
2617 bp
Loading structure...
UTR
Exon/CDS
Intron
Rmu_sc0000600.1_g000054.1.cds

Sequence Viewer

Length: 1068 bp
atgggtttcaccttttggttacttgcacatgcaaaggtcgaatctgtaaaatcaaaggaagtggctgaacagaggatgctcctgaagaacagctggcgtacgcctgatgaatctgatgatgatacttctgagggatcacagtcaccagaagaaagactgactgaagaggagtggcaagcaattaataagttactaagttacctgccagatgaaacattggcatctcactcaggaaacgacatgccgaatatgatccggtttatggtaactgtgtctgttgatcgggctgctgctaggatcgttgatataaatcacactgagattttgtgttgtaaatttgagcaacttcaagtgtctacgaagattaaacatcaaagtacatactgtgatgtgtcatgcagattctatggtctgtatgcaccggaaggcttgcttgctcagagcgtatctagtgagcagaaggcgaatgcactggctgctagctttgtataccatccagttggagagaatgttgattggaggctttcagctaccatttcaccttgtcatgtcacggttttgatggaaagctgcgatcgctttttggattttgtgaaaaggagtaatgcagttagtcctactgttacattggaaacgacaactgctttgcagatgcaaattgagaaagtgactcatagagcacaagagcaatttcaaatggtattagaagaacaaagcagatttgcccttgatattgatttggatgctccaaaagtaagaattcctatcagaaccaagggctcttccaaatgtgatagccattttctattggatcttggtcatttcaccctgcaaacgaaggatactcagcatgataaacagaagaagatcatgtattctcgtttttttataactggaagagatattgctgcattttttatggattgtggttccgattgccaaattcgcactttggaatcacctgattctgataaccacccaccggttaccccggaaactgttgacaatttctattctctcatagataggtgtggaatggctccacttgttgatcaggtaattacttag
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

355

Amino Acids

40.22

Weight (kDa)

5.19

Isoelectric Point (pI)

45.52

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 890
Acc36I ACCTGC 1 cut(s) 210
AccI GTMKAC 2 cut(s) 356, 489
AclWI GGATC 4 cut(s) 142, 247, 305, 819
AcsI RAATTY 3 cut(s) 335, 759, 942
AcuI CTGAAG 2 cut(s) 104, 183
AfaI GTAC 2 cut(s) 100, 379
AfiI CCNNNNNNNGG 2 cut(s) 262, 982
AgeI ACCGGT 1 cut(s) 982
AgsI TTSAA 2 cut(s) 350, 695
AluBI AGCT 4 cut(s) 93, 483, 530, 570
AluI AGCT 4 cut(s) 93, 483, 530, 570
Alw21I GWGCWC 1 cut(s) 682
AlwI GGATC 4 cut(s) 142, 247, 305, 819
ApeKI GCWGC 5 cut(s) 287, 290, 476, 570, 908
ApoI RAATTY 3 cut(s) 335, 759, 942
ArsI GACNNNNNNTTYG 2 cut(s) 628, 660
AseI ATTAAT 1 cut(s) 183
AsiGI ACCGGT 1 cut(s) 982
AsiSI GCGATCGC 1 cut(s) 577
AsuC2I CCSGG 1 cut(s) 992
AsuHPI GGTGA 4 cut(s) 135, 531, 817, 951
AsuNHI GCTAGC 1 cut(s) 479
BanII GRGCYC 1 cut(s) 782
Bbv12I GWGCWC 1 cut(s) 682
BbvI GCAGC 5 cut(s) 274, 277, 463, 557, 895
BccI CCATC 2 cut(s) 501, 556
BciVI GTATCC 1 cut(s) 835
BclI TGATCA 1 cut(s) 1051
BcnI CCSGG 1 cut(s) 992
BfaI CTAG 3 cut(s) 294, 450, 480
BfuAI ACCTGC 1 cut(s) 210
BfuI GTATCC 1 cut(s) 835
BisI GCNGC 5 cut(s) 288, 291, 477, 571, 909
BlsI GCNGC 5 cut(s) 289, 292, 478, 572, 910
Bme1390I CCNGG 1 cut(s) 992
BmiI GGNNCC 2 cut(s) 931, 1041
BmrFI CCNGG 1 cut(s) 992
BmsI GCATC 4 cut(s) 66, 230, 642, 733
BmtI GCTAGC 1 cut(s) 483
BpuMI CCSGG 1 cut(s) 992
BsaBI GATNNNNATC 1 cut(s) 309
BsaJI CCNNGG 2 cut(s) 774, 990
BsaWI WCCGGW 3 cut(s) 255, 421, 982
BsaXI ACNNNNNCTCC 2 cut(s) 495, 525
Bsc4I CCNNNNNNNGG 2 cut(s) 262, 982
Bse118I RCCGGY 1 cut(s) 982
Bse1I ACTGG 3 cut(s) 477, 497, 898
Bse8I GATNNNNATC 1 cut(s) 309
BseDI CCNNGG 2 cut(s) 774, 990
BseGI GGATG 3 cut(s) 81, 493, 748
BseJI GATNNNNATC 1 cut(s) 309
BseLI CCNNNNNNNGG 2 cut(s) 262, 982
BseMII CTCAG 5 cut(s) 120, 243, 309, 452, 860
BseNI ACTGG 3 cut(s) 477, 497, 898
BseRI GAGGAG 1 cut(s) 182
BseXI GCAGC 5 cut(s) 274, 277, 463, 557, 895
Bsh1285I CGRYCG 1 cut(s) 577
BshTI ACCGGT 1 cut(s) 982
BsiEI CGRYCG 1 cut(s) 577
BsiHKAI GWGCWC 1 cut(s) 682
BsiSI CCGG 4 cut(s) 256, 422, 983, 992
BsiWI CGTACG 1 cut(s) 98
BslI CCNNNNNNNGG 2 cut(s) 262, 982
BsmI GAATGC 1 cut(s) 472
Bsp1286I GDGCHC 2 cut(s) 682, 782
Bsp143I GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
BspCNI CTCAG 5 cut(s) 121, 242, 310, 451, 859
BspLI GGNNCC 2 cut(s) 931, 1041
BspMI ACCTGC 1 cut(s) 210
BspOI GCTAGC 1 cut(s) 483
BspPI GGATC 4 cut(s) 142, 247, 305, 819
BspQI GCTCTTC 1 cut(s) 787
BsrFI RCCGGY 1 cut(s) 982
BsrI ACTGG 3 cut(s) 477, 497, 898
BssAI RCCGGY 1 cut(s) 982
BssECI CCNNGG 2 cut(s) 774, 990
BssMI GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
BssNAI GTATAC 1 cut(s) 490
BssT1I CCWWGG 1 cut(s) 774
Bst1107I GTATAC 1 cut(s) 490
Bst4CI ACNGT 6 cut(s) 141, 271, 386, 556, 622, 1000
Bst6I CTCTTC 3 cut(s) 159, 787, 892
BstAPI GCANNNNNTGC 1 cut(s) 476
BstC8I GCNNGC 5 cut(s) 95, 177, 431, 435, 481
BstDEI CTNAG 7 cut(s) 129, 194, 229, 318, 438, 846, 1065
BstEII GGTNACC 1 cut(s) 985
BstF5I GGATG 3 cut(s) 81, 493, 748
BstKTI GATC 8 cut(s) 137, 255, 283, 300, 577, 814, 870, 1054
BstMBI GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
BstMCI CGRYCG 1 cut(s) 577
BstMWI GCNNNNNNNGC 3 cut(s) 476, 576, 945
BstNSI RCATGY 2 cut(s) 32, 244
BstPI GGTNACC 1 cut(s) 985
BstSCI CCNGG 1 cut(s) 990
BstV1I GCAGC 5 cut(s) 274, 277, 463, 557, 895
BstX2I RGATCY 1 cut(s) 811
BstXI CCANNNNNNTGG 1 cut(s) 500
BstYI RGATCY 1 cut(s) 811
BstZ17I GTATAC 1 cut(s) 490
BsuI GTATCC 1 cut(s) 835
BtsCI GGATG 3 cut(s) 81, 493, 748
BtsIMutI CAGTG 2 cut(s) 315, 470
BveI ACCTGC 1 cut(s) 210
Cac8I GCNNGC 5 cut(s) 95, 177, 431, 435, 481
Cfr10I RCCGGY 1 cut(s) 982
Csp6I GTAC 2 cut(s) 99, 378
CspAI ACCGGT 1 cut(s) 982
CspCI CAANNNNNGTGG 2 cut(s) 42, 77
CviAII CATG 6 cut(s) 29, 241, 396, 548, 851, 871
CviQI GTAC 2 cut(s) 99, 378
DdeI CTNAG 7 cut(s) 129, 194, 229, 318, 438, 846, 1065
DpnI GATC 8 cut(s) 136, 254, 282, 299, 576, 813, 869, 1053
DpnII GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
Eam1104I CTCTTC 3 cut(s) 159, 787, 892
EarI CTCTTC 3 cut(s) 159, 787, 892
Eco130I CCWWGG 1 cut(s) 774
Eco24I GRGCYC 1 cut(s) 782
Eco57I CTGAAG 2 cut(s) 104, 183
Eco91I GGTNACC 1 cut(s) 985
EcoO65I GGTNACC 1 cut(s) 985
EcoRI GAATTC 1 cut(s) 759
EcoT14I CCWWGG 1 cut(s) 774
EcoT38I GRGCYC 1 cut(s) 782
ErhI CCWWGG 1 cut(s) 774
FaeI CATG 6 cut(s) 32, 244, 399, 551, 854, 874
FalI AAGNNNNNCTT 2 cut(s) 417, 449
FatI CATG 6 cut(s) 28, 240, 395, 547, 850, 870
FbaI TGATCA 1 cut(s) 1051
FblI GTMKAC 2 cut(s) 356, 489
Fnu4HI GCNGC 5 cut(s) 288, 291, 477, 571, 909
FokI GGATG 3 cut(s) 88, 480, 755
FriOI GRGCYC 1 cut(s) 782
Fsp4HI GCNGC 5 cut(s) 288, 291, 477, 571, 909
FspBI CTAG 3 cut(s) 294, 450, 480
GluI GCNGC 5 cut(s) 288, 291, 477, 571, 909
HapII CCGG 4 cut(s) 256, 422, 983, 992
Hin1II CATG 6 cut(s) 32, 244, 399, 551, 854, 874
HincII GTYRAC 1 cut(s) 1003
HindII GTYRAC 1 cut(s) 1003
HinfI GANTC 6 cut(s) 41, 110, 402, 670, 956, 965
HpaII CCGG 4 cut(s) 256, 422, 983, 992
HphI GGTGA 4 cut(s) 135, 531, 817, 951
Hpy166II GTNNAC 3 cut(s) 357, 490, 1003
Hpy188I TCNGA 6 cut(s) 115, 130, 441, 770, 934, 970
Hpy188III TCNNGA 2 cut(s) 82, 231
Hpy8I GTNNAC 3 cut(s) 357, 490, 1003
HpyAV CCTTC 3 cut(s) 419, 454, 832
HpyCH4III ACNGT 6 cut(s) 141, 271, 386, 556, 622, 1000
HpyF10VI GCNNNNNNNGC 3 cut(s) 476, 576, 945
HpyF3I CTNAG 7 cut(s) 129, 194, 229, 318, 438, 846, 1065
Hsp92II CATG 6 cut(s) 32, 244, 399, 551, 854, 874
Ksp22I TGATCA 1 cut(s) 1051
Kzo9I GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
LguI GCTCTTC 1 cut(s) 787
LmnI GCTCC 3 cut(s) 84, 751, 1045
Lsp1109I GCAGC 5 cut(s) 274, 277, 463, 557, 895
LweI GCATC 4 cut(s) 66, 230, 642, 733
MaeI CTAG 3 cut(s) 294, 450, 480
MaeIII GTNAC 9 cut(s) 18, 141, 189, 197, 265, 550, 622, 667, 985
MalI GATC 8 cut(s) 136, 254, 282, 299, 576, 813, 869, 1053
MboI GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
MboII GAAGA 9 cut(s) 97, 161, 176, 373, 719, 774, 874, 877, 909
MflI RGATCY 1 cut(s) 811
MhlI GDGCHC 2 cut(s) 682, 782
MluCI AATT 8 cut(s) 180, 335, 657, 689, 759, 942, 1006, 1059
MlyI GAGTC 1 cut(s) 664
MmeI TCCRAC 1 cut(s) 481
MnlI CCTC 4 cut(s) 66, 124, 160, 513
MseI TTAA 2 cut(s) 183, 366
MspA1I CMGCKG 1 cut(s) 93
MspI CCGG 4 cut(s) 256, 422, 983, 992
MspR9I CCNGG 1 cut(s) 992
Mva1269I GAATGC 1 cut(s) 472
MwoI GCNNNNNNNGC 3 cut(s) 476, 576, 945
NciI CCSGG 1 cut(s) 992
NdeII GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
NheI GCTAGC 1 cut(s) 479
NlaIII CATG 6 cut(s) 32, 244, 399, 551, 854, 874
NlaIV GGNNCC 2 cut(s) 931, 1041
NmuCI GTSAC 3 cut(s) 141, 550, 667
NspI RCATGY 2 cut(s) 32, 244
PciSI GCTCTTC 1 cut(s) 787
PctI GAATGC 1 cut(s) 472
PfeI GAWTC 5 cut(s) 41, 110, 402, 956, 965
Pfl23II CGTACG 1 cut(s) 98
PinAI ACCGGT 1 cut(s) 982
PkrI GCNGC 5 cut(s) 289, 292, 478, 572, 910
Ple19I CGATCG 1 cut(s) 577
PleI GAGTC 1 cut(s) 664
PpsI GAGTC 1 cut(s) 664
PshBI ATTAAT 1 cut(s) 183
PsiI TTATAA 1 cut(s) 890
PspEI GGTNACC 1 cut(s) 985
PspLI CGTACG 1 cut(s) 98
PspN4I GGNNCC 2 cut(s) 931, 1041
PsuI RGATCY 1 cut(s) 811
PvuI CGATCG 1 cut(s) 577
PvuII CAGCTG 1 cut(s) 93
RgaI GCGATCGC 1 cut(s) 577
RsaI GTAC 2 cut(s) 100, 379
RsaNI GTAC 2 cut(s) 99, 378
SapI GCTCTTC 1 cut(s) 787
SaqAI TTAA 2 cut(s) 183, 366
SatI GCNGC 5 cut(s) 288, 291, 477, 571, 909
Sau3AI GATC 8 cut(s) 134, 252, 280, 297, 574, 811, 867, 1051
SchI GAGTC 1 cut(s) 664
ScrFI CCNGG 1 cut(s) 992
SduI GDGCHC 2 cut(s) 682, 782
SfaAI GCGATCGC 1 cut(s) 577
SfaNI GCATC 4 cut(s) 66, 230, 642, 733
SgfI GCGATCGC 1 cut(s) 577
Sse9I AATT 8 cut(s) 180, 335, 657, 689, 759, 942, 1006, 1059
SspMI CTAG 3 cut(s) 294, 450, 480
StyD4I CCNGG 1 cut(s) 990
StyI CCWWGG 1 cut(s) 774
TaaI ACNGT 6 cut(s) 141, 271, 386, 556, 622, 1000
TaqI TCGA 1 cut(s) 39
TasI AATT 8 cut(s) 180, 335, 657, 689, 759, 942, 1006, 1059
TatI WGTACW 1 cut(s) 377
TfiI GAWTC 5 cut(s) 41, 110, 402, 956, 965
Tru1I TTAA 2 cut(s) 183, 366
Tru9I TTAA 2 cut(s) 183, 366
TscAI CASTG 2 cut(s) 322, 477
TseFI GTSAC 3 cut(s) 141, 550, 667
TseI GCWGC 5 cut(s) 287, 290, 476, 570, 908
Tsp45I GTSAC 3 cut(s) 141, 550, 667
TspDTI ATGAA 2 cut(s) 123, 225
TspRI CASTG 2 cut(s) 322, 477
VspI ATTAAT 1 cut(s) 183
XapI RAATTY 3 cut(s) 335, 759, 942
XceI RCATGY 2 cut(s) 32, 244
XmiI GTMKAC 2 cut(s) 356, 489
XspI CTAG 3 cut(s) 294, 450, 480
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.