FvH4_5g06820

Pfam:DUF3633

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000415
Physical Location & Seq
Forward (+)
4013130 .. 4014696
1567 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06820.t1

Sequence Viewer

Length: 465 bp
ATGGAATTGCTTAAAATTGTCGTCGTCTTTATCACTATCATTCAGAATAATGGCTATTTTCGTCAATTTTTCCTACGTAGAATTCAAACAATAGAAAAATGTTCAAGAAGTGGAGGAGATACTAAAAAGGTCGTGAAGTTTGTACTAGGACGCGCAATCCTACTTTTATTCGGATTACCAGATGTAGAAATGGGAGAAATCTTGGCACATGAGATGATGCATGCATGGATCCACCTTCAAGGTGTACCTCGTGGATGCTGTGAACCTAGCACTGAAGAAGGGATATGTCAAGTAATGTCTTACAAATGGTTGCAGTGGTTTTCTTCTAGTGGTTTTGATAACTCGCACAAAACCAATAAGCAAGTTCAATATACAAGAGAGCTGAAGGAGTTATTAGTAGAAAGAATTCGGTGTCAAGATGACGAGGTTTATGGCCAAGGATTCAGAGATACTATGAGCTATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

155

Amino Acids

17.96

Weight (kDa)

7.65

Isoelectric Point (pI)

45.22

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 40 - 151 1e-29 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 153
AclWI GGATC 2 cut(s) 223, 236
AcoI YGGCCR 1 cut(s) 433
AcsI RAATTY 2 cut(s) 81, 405
AcuI CTGAAG 2 cut(s) 294, 404
AfaI GTAC 2 cut(s) 144, 246
AgsI TTSAA 4 cut(s) 86, 105, 239, 368
AluBI AGCT 2 cut(s) 382, 459
AluI AGCT 2 cut(s) 382, 459
AlwI GGATC 2 cut(s) 223, 236
AoxI GGCC 1 cut(s) 433
ApoI RAATTY 2 cut(s) 81, 405
Asp700I GAANNNNTTC 1 cut(s) 405
AspLEI GCGC 1 cut(s) 155
BalI TGGCCA 1 cut(s) 435
BamHI GGATCC 1 cut(s) 228
BauI CACGAG 1 cut(s) 249
BfaI CTAG 3 cut(s) 146, 267, 327
BmiI GGNNCC 1 cut(s) 230
BmsI GCATC 2 cut(s) 207, 245
BsaAI YACGTR 1 cut(s) 77
BsaJI CCNNGG 1 cut(s) 436
BseDI CCNNGG 1 cut(s) 436
BseGI GGATG 1 cut(s) 260
BseRI GAGGAG 1 cut(s) 129
Bsh1236I CGCG 1 cut(s) 153
BshFI GGCC 1 cut(s) 435
BsnI GGCC 1 cut(s) 435
Bsp143I GATC 1 cut(s) 228
BspANI GGCC 1 cut(s) 435
BspFNI CGCG 1 cut(s) 153
BspLI GGNNCC 1 cut(s) 230
BspPI GGATC 2 cut(s) 223, 236
BssECI CCNNGG 1 cut(s) 436
BssMI GATC 1 cut(s) 228
BssSI CACGAG 1 cut(s) 249
BssT1I CCWWGG 1 cut(s) 436
Bst2BI CACGAG 1 cut(s) 249
BstBAI YACGTR 1 cut(s) 77
BstC8I GCNNGC 1 cut(s) 222
BstF5I GGATG 1 cut(s) 260
BstFNI CGCG 1 cut(s) 153
BstHHI GCGC 1 cut(s) 155
BstKTI GATC 1 cut(s) 231
BstMBI GATC 1 cut(s) 228
BstNSI RCATGY 1 cut(s) 224
BstSNI TACGTA 1 cut(s) 77
BstUI CGCG 1 cut(s) 153
BstX2I RGATCY 1 cut(s) 228
BstYI RGATCY 1 cut(s) 228
BsuRI GGCC 1 cut(s) 435
BtsCI GGATG 1 cut(s) 260
BtsI GCAGTG 1 cut(s) 320
BtsIMutI CAGTG 2 cut(s) 270, 320
Cac8I GCNNGC 1 cut(s) 222
CfoI GCGC 1 cut(s) 155
CseI GACGC 1 cut(s) 159
Csp6I GTAC 2 cut(s) 143, 245
CviAII CATG 3 cut(s) 209, 221, 225
CviJI RGCY 4 cut(s) 54, 382, 435, 459
CviKI_1 RGCY 4 cut(s) 54, 382, 435, 459
CviQI GTAC 2 cut(s) 143, 245
DpnI GATC 1 cut(s) 230
DpnII GATC 1 cut(s) 228
EaeI YGGCCR 1 cut(s) 433
Eco105I TACGTA 1 cut(s) 77
Eco130I CCWWGG 1 cut(s) 436
Eco57I CTGAAG 2 cut(s) 294, 404
EcoRI GAATTC 2 cut(s) 81, 405
EcoT14I CCWWGG 1 cut(s) 436
EcoT22I ATGCAT 2 cut(s) 222, 226
ErhI CCWWGG 1 cut(s) 436
FaeI CATG 3 cut(s) 212, 224, 228
FaiI YATR 7 cut(s) 210, 222, 226, 286, 372, 432, 455
FatI CATG 3 cut(s) 208, 220, 224
FokI GGATG 1 cut(s) 267
FspBI CTAG 3 cut(s) 146, 267, 327
GlaI GCGC 1 cut(s) 154
HaeIII GGCC 1 cut(s) 435
HgaI GACGC 1 cut(s) 159
HhaI GCGC 1 cut(s) 155
Hin1II CATG 3 cut(s) 212, 224, 228
Hin6I GCGC 1 cut(s) 153
HinP1I GCGC 1 cut(s) 153
HinfI GANTC 1 cut(s) 441
Hpy166II GTNNAC 2 cut(s) 245, 263
Hpy188I TCNGA 3 cut(s) 45, 173, 446
Hpy188III TCNNGA 3 cut(s) 105, 133, 416
Hpy8I GTNNAC 2 cut(s) 245, 263
Hpy99I CGWCG 1 cut(s) 26
HpyAV CCTTC 3 cut(s) 245, 272, 379
HpyCH4IV ACGT 1 cut(s) 76
HpyCH4V TGCA 3 cut(s) 220, 224, 313
HpySE526I ACGT 1 cut(s) 76
Hsp92II CATG 3 cut(s) 212, 224, 228
HspAI GCGC 1 cut(s) 153
Kzo9I GATC 1 cut(s) 228
LpnPI CCDG 1 cut(s) 192
LweI GCATC 2 cut(s) 207, 245
MaeI CTAG 3 cut(s) 146, 267, 327
MaeII ACGT 1 cut(s) 76
MalI GATC 1 cut(s) 230
MboI GATC 1 cut(s) 228
MboII GAAGA 2 cut(s) 287, 315
MflI RGATCY 1 cut(s) 228
MlsI TGGCCA 1 cut(s) 435
MluCI AATT 5 cut(s) 5, 15, 65, 81, 405
MluNI TGGCCA 1 cut(s) 435
MnlI CCTC 3 cut(s) 107, 258, 418
Mox20I TGGCCA 1 cut(s) 435
Mph1103I ATGCAT 2 cut(s) 222, 226
MroXI GAANNNNTTC 1 cut(s) 405
MscI TGGCCA 1 cut(s) 435
MseI TTAA 1 cut(s) 12
Msp20I TGGCCA 1 cut(s) 435
MvnI CGCG 1 cut(s) 153
NdeII GATC 1 cut(s) 228
NlaIII CATG 3 cut(s) 212, 224, 228
NlaIV GGNNCC 1 cut(s) 230
NsiI ATGCAT 2 cut(s) 222, 226
NspI RCATGY 1 cut(s) 224
PaeI GCATGC 1 cut(s) 224
PdmI GAANNNNTTC 1 cut(s) 405
PfeI GAWTC 1 cut(s) 441
Ppu21I YACGTR 1 cut(s) 77
PspN4I GGNNCC 1 cut(s) 230
PsuI RGATCY 1 cut(s) 228
RsaI GTAC 2 cut(s) 144, 246
RsaNI GTAC 2 cut(s) 143, 245
SaqAI TTAA 1 cut(s) 12
Sau3AI GATC 1 cut(s) 228
SetI ASST 9 cut(s) 79, 132, 237, 244, 250, 268, 384, 429, 461
SfaNI GCATC 2 cut(s) 207, 245
SnaBI TACGTA 1 cut(s) 77
SphI GCATGC 1 cut(s) 224
Sse9I AATT 5 cut(s) 5, 15, 65, 81, 405
SspMI CTAG 3 cut(s) 146, 267, 327
StyI CCWWGG 1 cut(s) 436
TaiI ACGT 1 cut(s) 79
TasI AATT 5 cut(s) 5, 15, 65, 81, 405
TatI WGTACW 1 cut(s) 142
TfiI GAWTC 1 cut(s) 441
Tru1I TTAA 1 cut(s) 12
Tru9I TTAA 1 cut(s) 12
TscAI CASTG 2 cut(s) 277, 320
TspRI CASTG 2 cut(s) 277, 320
XapI RAATTY 2 cut(s) 81, 405
XceI RCATGY 1 cut(s) 224
XmnI GAANNNNTTC 1 cut(s) 405
XspI CTAG 3 cut(s) 146, 267, 327
Zsp2I ATGCAT 2 cut(s) 222, 226
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.