Rh4AG146300

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr4A
OG0000415
Physical Location & Seq
Forward (+)
36996999 .. 37005216
8218 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh4AG146300.1

Sequence Viewer

Length: 2208 bp
ATGACTATATGCACCATTCAAATGAGAGAAGAATATTTGAGATCTGTAATAAATATTGAAAAAGCCGTGAAGTTGCTGCTAGTAGACAGAATCATAGTTTTGTTTGGGAAACCAGATGTAGAAATGGGAGGATTGTTGGCACATGAGATGATGCATGGATGGTTGAATCTTCAAGGTAAACAATGGGAATGGGAACGAAGCGTAAAAGAAGGTATTTGCCAAGTAATGTATTACAAATGGTTGCAATGGTTTTCTTCTAGAGGTTATAACTCTTCGCACAAAACCAATGAGCAAGTTCAACATACAAGAATGTTGAAAGAGTACTTGGCACAGAAGATTGAGTGTTGGAATGATGAAGTTTATGGTCAAGGATTCATAAATGCTATGCGTGCTATTGAAATAGTTGGATTCAAAACTACGGTGGATCATATCGTCAAAGATGGAACTCTCCCTGTGGTTCCACCGGTTGCCCCGGTTGTGATGGGGAAGAAGAAGAAGACCACCACCACCACCACTGAGTTAACGAGGAAGAAGACCACCTGTGCTCCAATAACTGAGCTGTTATGGAAGGCGACTAGCAGCACAACTAAATCATTATGGAAGGCCACAGCTGCAACAATTACCAACTTATTATGGAAGGTCACAGCCGCCTGTGAAACACCGCGATTTGTTACTATGGAAGGACACCGCCACCACCAGTATCATCAGGGACAAAGCCATAACAACTTACATGTTCCCATGGCCGCATCTCCTACTCTCCTTCTTAGCTGCAATCAAAATCAAAGGTTACTTGCACATGCAAAGGTCGAATCTGTAAAATCAAACGAAGTGGCTGAACAGAGGATGCTCCTGAAGAAAAGCTGGTTTTCATTTAGATGGCGTACACCTGATGAATCTAATGATGATACTTCTGAGGGATCACAGTCACCAGAAGAAAGACTGACTGAAGAGGAGTGGCAAGCAATTAATAAGTTACTAAGTTACCAGCCAGATGAAACATTGGCATCTCACTCAGGAAAGGACATGCCGAATATGATCCGGTTTATGATTCTATGGTCTGTATGCACCGGAAGGCTCGCTTGCTCAAGCGTATCTAGTGAGCAGAAGGCAAATGCACTGGCCGCTAGCTTTGTATACCATCCAGTTGGAGAGAATGTTGATCGGAGGCTTTCAGCTACCATTTCACCTTGTCATGTCACGGTTTTGATGGAAAGCTGCGATCGCTTTTTGGATTTTGTGAAAAGGAGTAATGCAGTTAGTCCTACTGTTACATTGGAAACGACAACTGCTTTGCAGATGCAGATTGAGAAAGTGACTCATAGAGCACAAGAGCAATTTCAAATGGTATTAGAAGAACAAAGCAGATTTGCCCTTGATATTGATTTGGATGCTCCAAAAGTAAGAATTCCTATCAGAACCAAGGGCTCTTCCAAATGTGATAGCCATTTTCTATTGGATCTTGGTCATTTCACCCTGCAAACGAAGGATACTCAGCATGATAAACAGAAGAAGAACATGTATTCTCGTTTTTTTATAACTGGAAGAGATATTGCTGCATTTTTTATGGATTGTGGTTCCGATTGCCAAATTCGCACTTTGGAATCACCTGATTCTGATAACCACCCACCGGTTACCCTGGAAACTGTTGACAATTTCTATTCTCTCATAGATAGGTGTGGAATGGCTCCACTTGTTGATCAGATCAGAGTACCTCACCCAAGTTACCTGTCAATGCGTATTTCAATTCAAGAGCCAAACCTCGGAAACGTGCCCCATGGAGTCCTGCAGATCTTTTACACGGATGCTAGAATTTTAGTATGGAAGGGGATTGGCAATTCTGTGGCTACATGGCAACCTTGTTTTCTTGTGTTGTCGGGGACAAATATTTGTGTTTTTGGAATCCGAGAAGTCGCAGACTTATCAGCGATACTCCAGCATGGCTGGTGGACAAGGGGATGGACACTCAAAAGGCTCCCTCTTCAGTTGATGTCTTGGGAGAAACAATTGATTGTGTTGCTGAGCCTCAGACCGGCAATTCTAAAACAGCTGACCTTGTCATTAATGGGGCATTGGTGGAGACAAAGTTATCTATTTATGGAAAGACGGGTGATAAAGGTGCTGAAGAACTTGATGAGACTGATGCTTGAGGTTCTTGCCAGTTGGGGGGCAGGTTCATATGATTCTTTGGGAAGGTGGCATGACACTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

735

Amino Acids

84.12

Weight (kDa)

8.7

Isoelectric Point (pI)

47.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 18 - 145 3.8e-27 Protein DA1
VPS13_N PF12624 365 - 503 7.9e-08 VPS13-like family N-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 267, 1535
Acc36I ACCTGC 1 cut(s) 2159
AccI GTMKAC 2 cut(s) 84, 1134
AccII CGCG 1 cut(s) 664
AciI CCGC 5 cut(s) 648, 662, 688, 744, 1122
AclWI GGATC 4 cut(s) 432, 925, 1030, 1464
AcoI YGGCCR 2 cut(s) 741, 1119
AcsI RAATTY 3 cut(s) 1404, 1587, 1809
AcuI CTGAAG 4 cut(s) 872, 966, 1964, 2141
AfaI GTAC 3 cut(s) 323, 883, 1710
AfiI CCNNNNNNNGG 4 cut(s) 1627, 1760, 2029, 2163
AflIII ACRYGT 2 cut(s) 730, 1515
AgeI ACCGGT 2 cut(s) 463, 1627
AjnI CCWGG 1 cut(s) 1635
AjuI GAANNNNNNNTTGG 2 cut(s) 308, 340
AluBI AGCT 8 cut(s) 559, 611, 768, 861, 1128, 1175, 1215, 2047
AluI AGCT 8 cut(s) 559, 611, 768, 861, 1128, 1175, 1215, 2047
Alw21I GWGCWC 2 cut(s) 547, 1327
Alw26I GTCTC 2 cut(s) 2071, 2128
AlwI GGATC 4 cut(s) 432, 925, 1030, 1464
AoxI GGCC 3 cut(s) 603, 741, 1119
ApeKI GCWGC 6 cut(s) 76, 579, 611, 768, 1215, 1553
ApoI RAATTY 3 cut(s) 1404, 1587, 1809
ArsI GACNNNNNNTTYG 2 cut(s) 1273, 1305
AseI ATTAAT 2 cut(s) 966, 2060
AsiGI ACCGGT 2 cut(s) 463, 1627
AsiSI GCGATCGC 1 cut(s) 1222
AsuC2I CCSGG 1 cut(s) 473
AsuHPI GGTGA 6 cut(s) 918, 1176, 1462, 1596, 1706, 2119
AsuNHI GCTAGC 1 cut(s) 1124
BaeGI GKGCMC 1 cut(s) 1773
BanII GRGCYC 1 cut(s) 1427
BbsI GAAGAC 2 cut(s) 503, 539
Bbv12I GWGCWC 2 cut(s) 547, 1327
BbvI GCAGC 6 cut(s) 63, 591, 598, 755, 1202, 1540
BccI CCATC 7 cut(s) 153, 434, 475, 870, 1146, 1201, 1950
BceAI ACGGC 1 cut(s) 50
BciT130I CCWGG 1 cut(s) 1637
BciVI GTATCC 1 cut(s) 1480
BclI TGATCA 1 cut(s) 1696
BcnI CCSGG 1 cut(s) 473
BcoDI GTCTC 2 cut(s) 2071, 2128
BfaI CTAG 6 cut(s) 80, 258, 576, 1095, 1125, 1806
BfmI CTRYAG 1 cut(s) 1784
BfuAI ACCTGC 1 cut(s) 2159
BfuI GTATCC 1 cut(s) 1480
BglII AGATCT 2 cut(s) 41, 1788
BisI GCNGC 9 cut(s) 77, 580, 612, 648, 744, 769, 1122, 1216, 1554
BlpI GCTNAGC 1 cut(s) 2018
BlsI GCNGC 9 cut(s) 78, 581, 613, 649, 745, 770, 1123, 1217, 1555
BmcAI AGTACT 1 cut(s) 323
Bme1390I CCNGG 2 cut(s) 473, 1637
BmiI GGNNCC 4 cut(s) 459, 1576, 1686, 1973
BmrFI CCNGG 2 cut(s) 473, 1637
BmsI GCATC 8 cut(s) 141, 755, 834, 1013, 1287, 1378, 1792, 2130
BmtI GCTAGC 1 cut(s) 1128
BpiI GAAGAC 2 cut(s) 503, 539
BpmI CTGGAG 1 cut(s) 1916
Bpu1102I GCTNAGC 1 cut(s) 2018
BpuEI CTTGAG 2 cut(s) 1069, 2165
BpuMI CCSGG 1 cut(s) 473
BsaJI CCNNGG 6 cut(s) 471, 738, 1419, 1635, 1759, 1774
BsaWI WCCGGW 4 cut(s) 463, 1038, 1067, 1627
BsaXI ACNNNNNCTCC 6 cut(s) 529, 559, 1140, 1170, 2068, 2098
Bsc4I CCNNNNNNNGG 4 cut(s) 1627, 1760, 2029, 2163
Bse118I RCCGGY 3 cut(s) 463, 1627, 2029
Bse1I ACTGG 5 cut(s) 697, 1122, 1142, 1543, 2157
Bse3DI GCAATG 1 cut(s) 251
BseBI CCWGG 1 cut(s) 1637
BseDI CCNNGG 6 cut(s) 471, 738, 1419, 1635, 1759, 1774
BseGI GGATG 6 cut(s) 164, 849, 1138, 1393, 1807, 1961
BseLI CCNNNNNNNGG 4 cut(s) 1627, 1760, 2029, 2163
BseMI GCAATG 1 cut(s) 251
BseMII CTCAG 7 cut(s) 507, 546, 903, 1026, 1505, 2009, 2038
BseNI ACTGG 5 cut(s) 697, 1122, 1142, 1543, 2157
BseRI GAGGAG 1 cut(s) 965
BseSI GKGCMC 1 cut(s) 1773
BseXI GCAGC 6 cut(s) 63, 591, 598, 755, 1202, 1540
Bsh1236I CGCG 1 cut(s) 664
Bsh1285I CGRYCG 1 cut(s) 1222
BshFI GGCC 3 cut(s) 605, 743, 1121
BshTI ACCGGT 2 cut(s) 463, 1627
BsiEI CGRYCG 1 cut(s) 1222
BsiHKAI GWGCWC 2 cut(s) 547, 1327
BsiSI CCGG 6 cut(s) 464, 473, 1039, 1068, 1628, 2030
BslFI GGGAC 2 cut(s) 723, 1891
BslI CCNNNNNNNGG 4 cut(s) 1627, 1760, 2029, 2163
BsmAI GTCTC 2 cut(s) 2071, 2128
BsmFI GGGAC 2 cut(s) 723, 1891
BsnI GGCC 3 cut(s) 605, 743, 1121
Bsp1286I GDGCHC 4 cut(s) 547, 1327, 1427, 1773
Bsp1720I GCTNAGC 1 cut(s) 2018
Bsp19I CCATGG 2 cut(s) 738, 1774
BspACI CCGC 5 cut(s) 648, 662, 688, 744, 1122
BspANI GGCC 3 cut(s) 605, 743, 1121
BspCNI CTCAG 7 cut(s) 508, 547, 904, 1025, 1504, 2010, 2037
BspFNI CGCG 1 cut(s) 664
BspLI GGNNCC 4 cut(s) 459, 1576, 1686, 1973
BspMAI CTGCAG 1 cut(s) 1788
BspMI ACCTGC 1 cut(s) 2159
BspOI GCTAGC 1 cut(s) 1128
BspPI GGATC 4 cut(s) 432, 925, 1030, 1464
BspQI GCTCTTC 1 cut(s) 1432
BsrDI GCAATG 1 cut(s) 251
BsrFI RCCGGY 3 cut(s) 463, 1627, 2029
BsrI ACTGG 5 cut(s) 697, 1122, 1142, 1543, 2157
BssAI RCCGGY 3 cut(s) 463, 1627, 2029
BssECI CCNNGG 6 cut(s) 471, 738, 1419, 1635, 1759, 1774
BssNAI GTATAC 1 cut(s) 1135
BssT1I CCWWGG 3 cut(s) 738, 1419, 1774
Bst1107I GTATAC 1 cut(s) 1135
Bst2UI CCWGG 1 cut(s) 1637
Bst4CI ACNGT 5 cut(s) 421, 924, 1201, 1267, 1645
Bst6I CTCTTC 5 cut(s) 277, 942, 1432, 1537, 1983
BstC8I GCNNGC 5 cut(s) 390, 960, 1077, 1081, 1126
BstDEI CTNAG 9 cut(s) 516, 555, 764, 912, 977, 1012, 1491, 2018, 2024
BstDSI CCRYGG 2 cut(s) 738, 1774
BstEII GGTNACC 1 cut(s) 1630
BstF5I GGATG 6 cut(s) 164, 849, 1138, 1393, 1807, 1961
BstFNI CGCG 1 cut(s) 664
BstMAI GTCTC 2 cut(s) 2071, 2128
BstMCI CGRYCG 1 cut(s) 1222
BstMWI GCNNNNNNNGC 5 cut(s) 389, 611, 1121, 1221, 1590
BstNI CCWGG 1 cut(s) 1637
BstNSI RCATGY 4 cut(s) 734, 800, 1027, 1519
BstPI GGTNACC 1 cut(s) 1630
BstSCI CCNGG 2 cut(s) 471, 1635
BstSFI CTRYAG 1 cut(s) 1784
BstSLI GKGCMC 1 cut(s) 1773
BstUI CGCG 1 cut(s) 664
BstV1I GCAGC 6 cut(s) 63, 591, 598, 755, 1202, 1540
BstV2I GAAGAC 2 cut(s) 503, 539
BstX2I RGATCY 3 cut(s) 41, 1456, 1788
BstXI CCANNNNNNTGG 1 cut(s) 1145
BstYI RGATCY 3 cut(s) 41, 1456, 1788
BstZ17I GTATAC 1 cut(s) 1135
BsuI GTATCC 1 cut(s) 1480
BsuRI GGCC 3 cut(s) 605, 743, 1121
BtgI CCRYGG 2 cut(s) 738, 1774
BtsCI GGATG 6 cut(s) 164, 849, 1138, 1393, 1807, 1961
BtsIMutI CAGTG 2 cut(s) 513, 1115
BveI ACCTGC 1 cut(s) 2159
Cac8I GCNNGC 5 cut(s) 390, 960, 1077, 1081, 1126
Cfr10I RCCGGY 3 cut(s) 463, 1627, 2029
Csp6I GTAC 3 cut(s) 322, 882, 1709
CspAI ACCGGT 2 cut(s) 463, 1627
CspCI CAANNNNNGTGG 2 cut(s) 810, 845
CviQI GTAC 3 cut(s) 322, 882, 1709
DdeI CTNAG 9 cut(s) 516, 555, 764, 912, 977, 1012, 1491, 2018, 2024
EaeI YGGCCR 2 cut(s) 741, 1119
Eam1104I CTCTTC 5 cut(s) 277, 942, 1432, 1537, 1983
EarI CTCTTC 5 cut(s) 277, 942, 1432, 1537, 1983
Eco130I CCWWGG 3 cut(s) 738, 1419, 1774
Eco24I GRGCYC 1 cut(s) 1427
Eco57I CTGAAG 4 cut(s) 872, 966, 1964, 2141
Eco91I GGTNACC 1 cut(s) 1630
EcoO65I GGTNACC 1 cut(s) 1630
EcoRI GAATTC 1 cut(s) 1404
EcoRII CCWGG 1 cut(s) 1635
EcoT14I CCWWGG 3 cut(s) 738, 1419, 1774
EcoT22I ATGCAT 1 cut(s) 156
EcoT38I GRGCYC 1 cut(s) 1427
ErhI CCWWGG 3 cut(s) 738, 1419, 1774
FalI AAGNNNNNCTT 2 cut(s) 1063, 1095
FaqI GGGAC 2 cut(s) 723, 1891
FauNDI CATATG 1 cut(s) 2176
FbaI TGATCA 1 cut(s) 1696
FblI GTMKAC 2 cut(s) 84, 1134
Fnu4HI GCNGC 9 cut(s) 77, 580, 612, 648, 744, 769, 1122, 1216, 1554
FokI GGATG 6 cut(s) 171, 856, 1125, 1400, 1814, 1968
FriOI GRGCYC 1 cut(s) 1427
Fsp4HI GCNGC 9 cut(s) 77, 580, 612, 648, 744, 769, 1122, 1216, 1554
FspBI CTAG 6 cut(s) 80, 258, 576, 1095, 1125, 1806
GluI GCNGC 9 cut(s) 77, 580, 612, 648, 744, 769, 1122, 1216, 1554
GsuI CTGGAG 1 cut(s) 1916
HaeIII GGCC 3 cut(s) 605, 743, 1121
HapII CCGG 6 cut(s) 464, 473, 1039, 1068, 1628, 2030
HincII GTYRAC 2 cut(s) 522, 1648
HindII GTYRAC 2 cut(s) 522, 1648
HpaI GTTAAC 1 cut(s) 522
HpaII CCGG 6 cut(s) 464, 473, 1039, 1068, 1628, 2030
HphI GGTGA 6 cut(s) 918, 1176, 1462, 1596, 1706, 2119
Hpy166II GTNNAC 7 cut(s) 85, 179, 522, 884, 1135, 1648, 1947
Hpy188III TCNNGA 4 cut(s) 258, 850, 1014, 1748
Hpy8I GTNNAC 7 cut(s) 85, 179, 522, 884, 1135, 1648, 1947
HpyCH4III ACNGT 5 cut(s) 421, 924, 1201, 1267, 1645
HpyCH4IV ACGT 1 cut(s) 1767
HpyF10VI GCNNNNNNNGC 5 cut(s) 389, 611, 1121, 1221, 1590
HpyF3I CTNAG 9 cut(s) 516, 555, 764, 912, 977, 1012, 1491, 2018, 2024
HpySE526I ACGT 1 cut(s) 1767
Ksp22I TGATCA 1 cut(s) 1696
KspAI GTTAAC 1 cut(s) 522
LguI GCTCTTC 1 cut(s) 1432
LmnI GCTCC 5 cut(s) 550, 852, 1396, 1690, 1977
Lsp1109I GCAGC 6 cut(s) 63, 591, 598, 755, 1202, 1540
LweI GCATC 8 cut(s) 141, 755, 834, 1013, 1287, 1378, 1792, 2130
MaeI CTAG 6 cut(s) 80, 258, 576, 1095, 1125, 1806
MaeII ACGT 1 cut(s) 1767
MfeI CAATTG 1 cut(s) 2003
MflI RGATCY 3 cut(s) 41, 1456, 1788
MhlI GDGCHC 4 cut(s) 547, 1327, 1427, 1773
MlyI GAGTC 2 cut(s) 1309, 1788
MmeI TCCRAC 3 cut(s) 326, 385, 1126
Mph1103I ATGCAT 1 cut(s) 156
MseI TTAA 4 cut(s) 521, 966, 2060, 2206
MslI CAYNNNNRTG 2 cut(s) 20, 874
MspA1I CMGCKG 2 cut(s) 611, 2047
MspI CCGG 6 cut(s) 464, 473, 1039, 1068, 1628, 2030
MspR9I CCNGG 2 cut(s) 473, 1637
MunI CAATTG 1 cut(s) 2003
MvaI CCWGG 1 cut(s) 1637
MvnI CGCG 1 cut(s) 664
MwoI GCNNNNNNNGC 5 cut(s) 389, 611, 1121, 1221, 1590
NciI CCSGG 1 cut(s) 473
NcoI CCATGG 2 cut(s) 738, 1774
NdeI CATATG 1 cut(s) 2176
NheI GCTAGC 1 cut(s) 1124
NlaIV GGNNCC 4 cut(s) 459, 1576, 1686, 1973
NmuCI GTSAC 4 cut(s) 640, 924, 1195, 1312
NsiI ATGCAT 1 cut(s) 156
NspI RCATGY 4 cut(s) 734, 800, 1027, 1519
PciI ACATGT 2 cut(s) 730, 1515
PciSI GCTCTTC 1 cut(s) 1432
PflFI GACNNNGTC 1 cut(s) 2053
PinAI ACCGGT 2 cut(s) 463, 1627
PkrI GCNGC 9 cut(s) 78, 581, 613, 649, 745, 770, 1123, 1217, 1555
Ple19I CGATCG 1 cut(s) 1222
PleI GAGTC 2 cut(s) 1309, 1787
PpsI GAGTC 2 cut(s) 1309, 1787
PscI ACATGT 2 cut(s) 730, 1515
PshBI ATTAAT 2 cut(s) 966, 2060
PsiI TTATAA 2 cut(s) 267, 1535
Psp6I CCWGG 1 cut(s) 1635
PspEI GGTNACC 1 cut(s) 1630
PspGI CCWGG 1 cut(s) 1635
PspN4I GGNNCC 4 cut(s) 459, 1576, 1686, 1973
PstI CTGCAG 1 cut(s) 1788
PsuI RGATCY 3 cut(s) 41, 1456, 1788
PsyI GACNNNGTC 1 cut(s) 2053
PvuI CGATCG 1 cut(s) 1222
PvuII CAGCTG 2 cut(s) 611, 2047
RgaI GCGATCGC 1 cut(s) 1222
RsaI GTAC 3 cut(s) 323, 883, 1710
RsaNI GTAC 3 cut(s) 322, 882, 1709
RseI CAYNNNNRTG 2 cut(s) 20, 874
SapI GCTCTTC 1 cut(s) 1432
SaqAI TTAA 4 cut(s) 521, 966, 2060, 2206
SatI GCNGC 9 cut(s) 77, 580, 612, 648, 744, 769, 1122, 1216, 1554
ScaI AGTACT 1 cut(s) 323
SchI GAGTC 2 cut(s) 1309, 1788
ScrFI CCNGG 2 cut(s) 473, 1637
SduI GDGCHC 4 cut(s) 547, 1327, 1427, 1773
SfaAI GCGATCGC 1 cut(s) 1222
SfaNI GCATC 8 cut(s) 141, 755, 834, 1013, 1287, 1378, 1792, 2130
SfcI CTRYAG 1 cut(s) 1784
SgfI GCGATCGC 1 cut(s) 1222
SmiMI CAYNNNNRTG 2 cut(s) 20, 874
SmlI CTYRAG 2 cut(s) 1084, 2144
SmoI CTYRAG 2 cut(s) 1084, 2144
SsiI CCGC 5 cut(s) 648, 662, 688, 744, 1122
SspI AATATT 3 cut(s) 35, 55, 1885
SspMI CTAG 6 cut(s) 80, 258, 576, 1095, 1125, 1806
StyD4I CCNGG 2 cut(s) 471, 1635
StyI CCWWGG 3 cut(s) 738, 1419, 1774
TaaI ACNGT 5 cut(s) 421, 924, 1201, 1267, 1645
TaiI ACGT 1 cut(s) 1770
TaqI TCGA 1 cut(s) 807
TatI WGTACW 1 cut(s) 321
TauI GCSGC 3 cut(s) 650, 746, 1124
Tru1I TTAA 4 cut(s) 521, 966, 2060, 2206
Tru9I TTAA 4 cut(s) 521, 966, 2060, 2206
TscAI CASTG 2 cut(s) 520, 1122
TseFI GTSAC 4 cut(s) 640, 924, 1195, 1312
TseI GCWGC 6 cut(s) 76, 579, 611, 768, 1215, 1553
Tsp45I GTSAC 4 cut(s) 640, 924, 1195, 1312
TspDTI ATGAA 6 cut(s) 364, 369, 858, 906, 1008, 2163
TspGWI ACGGA 1 cut(s) 1814
TspRI CASTG 2 cut(s) 520, 1122
Tth111I GACNNNGTC 1 cut(s) 2053
VspI ATTAAT 2 cut(s) 966, 2060
XapI RAATTY 3 cut(s) 1404, 1587, 1809
XbaI TCTAGA 1 cut(s) 257
XceI RCATGY 4 cut(s) 734, 800, 1027, 1519
XmiI GTMKAC 2 cut(s) 84, 1134
XspI CTAG 6 cut(s) 80, 258, 576, 1095, 1125, 1806
ZrmI AGTACT 1 cut(s) 323
Zsp2I ATGCAT 1 cut(s) 156
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.