FvH4_5g06800

Pfam:DUF3633

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0000415
Physical Location & Seq
Forward (+)
4002555 .. 4004486
1932 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g06800.t1

Sequence Viewer

Length: 348 bp
ATGTCACCGAACGACGACTCTGAGGAAGTGAAACATGATAGCTATATGGCAAGCATTTTCCGGCCAGATTGTGATACTTGTTTCAGATCCAAGATAGAAAAATGTTCGAGAAGTGGAGGAGATACCAAAAAGGTCGTGAAGTTTGTACTAGGACGCGCAATCCTACTTTTATTCGGATTACCAGATGTGGAAATGGGAGAAATCTTGGCACATGAGATGATGCATGCATGGATCCACCTTCAAGGTGTACCTCGTGGATGCTGTGAACCTAGCACTGAAGAAGGGATATGTCAACTAATGTCTTACAAATGGTTGCAGTGGTTTTCTTCTAGTCGTTGCCGCTGCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

116

Amino Acids

13.12

Weight (kDa)

6.06

Isoelectric Point (pI)

75.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 42 - 109 3.4e-23 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 156
AciI CCGC 1 cut(s) 340
AclWI GGATC 3 cut(s) 81, 226, 239
AcoI YGGCCR 1 cut(s) 62
AcuI CTGAAG 1 cut(s) 297
AfaI GTAC 2 cut(s) 147, 249
AgsI TTSAA 1 cut(s) 242
AluBI AGCT 1 cut(s) 42
AluI AGCT 1 cut(s) 42
AlwI GGATC 3 cut(s) 81, 226, 239
AoxI GGCC 1 cut(s) 62
ApeKI GCWGC 1 cut(s) 342
AspLEI GCGC 1 cut(s) 158
BamHI GGATCC 1 cut(s) 231
BauI CACGAG 1 cut(s) 252
BbvI GCAGC 1 cut(s) 329
BfaI CTAG 3 cut(s) 149, 270, 330
BisI GCNGC 2 cut(s) 340, 343
BlsI GCNGC 2 cut(s) 341, 344
BmiI GGNNCC 1 cut(s) 233
BmsI GCATC 2 cut(s) 210, 248
BseGI GGATG 1 cut(s) 263
BseMII CTCAG 1 cut(s) 12
BseRI GAGGAG 1 cut(s) 132
BseXI GCAGC 1 cut(s) 329
Bsh1236I CGCG 1 cut(s) 156
BshFI GGCC 1 cut(s) 64
BsiSI CCGG 1 cut(s) 61
BsnI GGCC 1 cut(s) 64
Bsp143I GATC 2 cut(s) 86, 231
BspACI CCGC 1 cut(s) 340
BspANI GGCC 1 cut(s) 64
BspCNI CTCAG 1 cut(s) 13
BspFNI CGCG 1 cut(s) 156
BspLI GGNNCC 1 cut(s) 233
BspPI GGATC 3 cut(s) 81, 226, 239
BssMI GATC 2 cut(s) 86, 231
BssSI CACGAG 1 cut(s) 252
Bst2BI CACGAG 1 cut(s) 252
BstC8I GCNNGC 2 cut(s) 52, 225
BstDEI CTNAG 1 cut(s) 21
BstF5I GGATG 1 cut(s) 263
BstFNI CGCG 1 cut(s) 156
BstHHI GCGC 1 cut(s) 158
BstKTI GATC 2 cut(s) 89, 234
BstMBI GATC 2 cut(s) 86, 231
BstNSI RCATGY 1 cut(s) 227
BstUI CGCG 1 cut(s) 156
BstV1I GCAGC 1 cut(s) 329
BstX2I RGATCY 2 cut(s) 86, 231
BstYI RGATCY 2 cut(s) 86, 231
BsuRI GGCC 1 cut(s) 64
BtsCI GGATG 1 cut(s) 263
BtsI GCAGTG 1 cut(s) 323
BtsIMutI CAGTG 2 cut(s) 273, 323
Cac8I GCNNGC 2 cut(s) 52, 225
CfoI GCGC 1 cut(s) 158
CseI GACGC 1 cut(s) 162
Csp6I GTAC 2 cut(s) 146, 248
CviAII CATG 4 cut(s) 35, 212, 224, 228
CviJI RGCY 2 cut(s) 42, 64
CviKI_1 RGCY 2 cut(s) 42, 64
CviQI GTAC 2 cut(s) 146, 248
DdeI CTNAG 1 cut(s) 21
DpnI GATC 2 cut(s) 88, 233
DpnII GATC 2 cut(s) 86, 231
EaeI YGGCCR 1 cut(s) 62
Eco57I CTGAAG 1 cut(s) 297
EcoT22I ATGCAT 2 cut(s) 225, 229
FaeI CATG 4 cut(s) 38, 215, 227, 231
FaiI YATR 7 cut(s) 36, 45, 47, 213, 225, 229, 289
FatI CATG 4 cut(s) 34, 211, 223, 227
Fnu4HI GCNGC 2 cut(s) 340, 343
FokI GGATG 1 cut(s) 270
Fsp4HI GCNGC 2 cut(s) 340, 343
FspBI CTAG 3 cut(s) 149, 270, 330
GlaI GCGC 1 cut(s) 157
GluI GCNGC 2 cut(s) 340, 343
HaeIII GGCC 1 cut(s) 64
HapII CCGG 1 cut(s) 61
HgaI GACGC 1 cut(s) 162
HhaI GCGC 1 cut(s) 158
Hin1II CATG 4 cut(s) 38, 215, 227, 231
Hin6I GCGC 1 cut(s) 156
HinP1I GCGC 1 cut(s) 156
HincII GTYRAC 1 cut(s) 293
HindII GTYRAC 1 cut(s) 293
HinfI GANTC 1 cut(s) 17
HpaII CCGG 1 cut(s) 61
Hpy166II GTNNAC 3 cut(s) 248, 266, 293
Hpy188I TCNGA 3 cut(s) 22, 86, 176
Hpy188III TCNNGA 2 cut(s) 108, 136
Hpy8I GTNNAC 3 cut(s) 248, 266, 293
Hpy99I CGWCG 1 cut(s) 17
HpyAV CCTTC 2 cut(s) 248, 275
HpyCH4V TGCA 3 cut(s) 223, 227, 316
HpyF3I CTNAG 1 cut(s) 21
Hsp92II CATG 4 cut(s) 38, 215, 227, 231
HspAI GCGC 1 cut(s) 156
Kzo9I GATC 2 cut(s) 86, 231
LpnPI CCDG 3 cut(s) 74, 78, 195
Lsp1109I GCAGC 1 cut(s) 329
LweI GCATC 2 cut(s) 210, 248
MaeI CTAG 3 cut(s) 149, 270, 330
MaeIII GTNAC 1 cut(s) 3
MalI GATC 2 cut(s) 88, 233
MboI GATC 2 cut(s) 86, 231
MboII GAAGA 2 cut(s) 290, 318
MflI RGATCY 2 cut(s) 86, 231
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 3 cut(s) 16, 110, 261
Mph1103I ATGCAT 2 cut(s) 225, 229
MspA1I CMGCKG 1 cut(s) 342
MspI CCGG 1 cut(s) 61
MvnI CGCG 1 cut(s) 156
NdeII GATC 2 cut(s) 86, 231
NlaIII CATG 4 cut(s) 38, 215, 227, 231
NlaIV GGNNCC 1 cut(s) 233
NmuCI GTSAC 1 cut(s) 3
NsiI ATGCAT 2 cut(s) 225, 229
NspI RCATGY 1 cut(s) 227
PaeI GCATGC 1 cut(s) 227
PkrI GCNGC 2 cut(s) 341, 344
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PspN4I GGNNCC 1 cut(s) 233
PsuI RGATCY 2 cut(s) 86, 231
RsaI GTAC 2 cut(s) 147, 249
RsaNI GTAC 2 cut(s) 146, 248
SatI GCNGC 2 cut(s) 340, 343
Sau3AI GATC 2 cut(s) 86, 231
SchI GAGTC 1 cut(s) 11
SetI ASST 6 cut(s) 44, 135, 240, 247, 253, 271
SfaNI GCATC 2 cut(s) 210, 248
SphI GCATGC 1 cut(s) 227
SsiI CCGC 1 cut(s) 340
SspMI CTAG 3 cut(s) 149, 270, 330
TaqI TCGA 1 cut(s) 107
TatI WGTACW 1 cut(s) 145
TauI GCSGC 1 cut(s) 342
TscAI CASTG 2 cut(s) 280, 323
TseFI GTSAC 1 cut(s) 3
TseI GCWGC 1 cut(s) 342
Tsp45I GTSAC 1 cut(s) 3
TspRI CASTG 2 cut(s) 280, 323
XceI RCATGY 1 cut(s) 227
XspI CTAG 3 cut(s) 149, 270, 330
Zsp2I ATGCAT 2 cut(s) 225, 229
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.