Rorug03G0030400

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000415
Physical Location & Seq
Forward (+)
2401404 .. 2404870
3467 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0030400.1

Sequence Viewer

Length: 1131 bp
ATGGGAAGTGCTGGTGAAAGTGGTTATGGTGCATACACCTATGAGGCTCTTGAGAGGGAGCATTATTGGCCCTCAGAAAAACTCCGAATTTCCATTACTGGGGCTGGTGGCTTTATTGCCTCCCACATTGCCCGCCGATTGAAGAATGAGGGCCACTACATTATTGCCTCTGACTGGAAGAAGAATGAGCACATGAGTGAAGACATGTTCTGCCATGAATTCCATCTTGTGGACCTCAGGGTCATGGATAACTGCTTGAAGGTTACCAAGGATGTCGATCATGTGTTCAACCTTGCTGCTGATATGGGCGGCATGGGATTCATTCAGTCCAACCACTCTGTCATTATGTACAATAATACCATGATCAGCTTTAACATGCTGGAAGCTGCTAGGATCAGTGGAGTGAAAAGGTTATTTTATGCTTCCAGTGCTTGTATTTATCCCGAGTTTAAGCAGCTGGAAACCAATGTGAGCTTGAAGGAGGCTGATGCCTGGCCTGCAGAGCCTCAAGATGCTTATGGCTTAGAGAAACTTGCAACTGAGGAGTTGTGCAAGCACTACACCAAAGACTTTGGAATCGAATGCCGTATTGGAAGGTTCCACAACATCTATGGTCCTTTCGGAACCTGGAAAGGGGGCAGGGAGAAGGCTCCTGCTGCTTTCTGCAGAAAGGCTCTCACTTCCACTGATAAGTTTGAGATGTGGGGAGATGGACTCCAGACCCGATCCTTCACCTTTATTGATGAATGTGTTGAAGGTGTACTCCGGTTGACAAAATCCGACTTCCGTGAGCCAGTGAATATTGGAAGCGATGAGATGGTTAGCATGAATGAGATGGCTGAGATTGTTCTTAGCTTTGAGAACAAGAAGCTGCCTATTCAGCACATTCCTGGGCCTGAGGGTGTCCGTGGTCGTAACTCTGACAACACCCTGATCAAAGAGAAACTTGGCTGGGCTCCTACCATGAGGCTGAAGGATGGACTGAGGTTTACATACTTCTGGATCAAGGAACAGATTGAGAAAGAGAAAGCACAAGGTACTGATCTGTCGCTTTATGGGTCATCTAAGGTGGTGGGAACTCAAGCCCCAGTTCAACTTGGTTCACTTCGTGCCGCTGATGGCAAAGAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

376

Amino Acids

42.42

Weight (kDa)

6.05

Isoelectric Point (pI)

41.35

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Epimerase PF01370 30 - 269 1.6e-45 NAD dependent epimerase/dehydratase family
GDP_Man_Dehyd PF16363 32 - 329 1.3e-26 GDP-mannose 4,6 dehydratase
3Beta_HSD PF01073 32 - 184 9.7e-10 3-beta hydroxysteroid dehydrogenase/isomerase family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 239
AccB7I CCANNNNNTGG 1 cut(s) 229
AciI CCGC 3 cut(s) 133, 309, 1113
AclWI GGATC 3 cut(s) 401, 720, 1010
AcsI RAATTY 2 cut(s) 87, 218
AcuI CTGAAG 1 cut(s) 992
AdeI CACNNNGTG 1 cut(s) 1109
AfaI GTAC 3 cut(s) 350, 762, 1039
AfiI CCNNNNNNNGG 4 cut(s) 99, 174, 229, 633
AflIII ACRYGT 1 cut(s) 204
AgsI TTSAA 6 cut(s) 142, 259, 289, 478, 755, 1094
AjnI CCWGG 3 cut(s) 491, 626, 889
AjuI GAANNNNNNNTTGG 2 cut(s) 573, 605
AleI CACNNNNGTG 1 cut(s) 195
AluBI AGCT 6 cut(s) 369, 386, 457, 474, 855, 871
AluI AGCT 6 cut(s) 369, 386, 457, 474, 855, 871
Alw21I GWGCWC 1 cut(s) 192
AlwI GGATC 3 cut(s) 401, 720, 1010
Ama87I CYCGRG 1 cut(s) 443
AoxI GGCC 4 cut(s) 68, 151, 494, 893
ApeKI GCWGC 5 cut(s) 296, 386, 454, 656, 871
ApoI RAATTY 2 cut(s) 87, 218
AspS9I GGNCC 5 cut(s) 69, 151, 232, 614, 893
AsuHPI GGTGA 2 cut(s) 26, 724
AvaI CYCGRG 1 cut(s) 443
AvaII GGWCC 2 cut(s) 232, 614
AxyI CCTNAGG 2 cut(s) 236, 897
BanII GRGCYC 1 cut(s) 958
BbsI GAAGAC 1 cut(s) 207
Bbv12I GWGCWC 1 cut(s) 192
BbvI GCAGC 5 cut(s) 283, 373, 466, 643, 858
BccI CCATC 6 cut(s) 231, 704, 811, 829, 971, 1112
BceAI ACGGC 1 cut(s) 570
BciT130I CCWGG 3 cut(s) 493, 628, 891
BclI TGATCA 2 cut(s) 363, 933
BfaI CTAG 1 cut(s) 390
BfmI CTRYAG 2 cut(s) 498, 664
BisI GCNGC 7 cut(s) 297, 310, 387, 455, 657, 872, 1113
BlsI GCNGC 7 cut(s) 298, 311, 388, 456, 658, 873, 1114
Bme1390I CCNGG 3 cut(s) 493, 628, 891
Bme18I GGWCC 2 cut(s) 232, 614
BmeT110I CYCGRG 1 cut(s) 443
BmgT120I GGNCC 5 cut(s) 69, 151, 232, 614, 893
BmiI GGNNCC 4 cut(s) 599, 625, 651, 957
BmrFI CCNGG 3 cut(s) 493, 628, 891
BmrI ACTGGG 2 cut(s) 108, 1082
BmsI GCATC 2 cut(s) 478, 502
BmuI ACTGGG 2 cut(s) 108, 1082
BpiI GAAGAC 1 cut(s) 207
BplI GAGNNNNNCTC 2 cut(s) 699, 731
BpmI CTGGAG 1 cut(s) 701
BpuEI CTTGAG 3 cut(s) 71, 492, 1065
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 3 cut(s) 267, 890, 907
BsaWI WCCGGW 1 cut(s) 765
Bsc4I CCNNNNNNNGG 4 cut(s) 99, 174, 229, 633
Bse1I ACTGG 5 cut(s) 103, 179, 426, 794, 1088
Bse21I CCTNAGG 2 cut(s) 236, 897
Bse3DI GCAATG 1 cut(s) 126
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 3 cut(s) 493, 628, 891
BseDI CCNNGG 3 cut(s) 267, 890, 907
BseGI GGATG 2 cut(s) 277, 982
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 4 cut(s) 99, 174, 229, 633
BseMI GCAATG 1 cut(s) 126
BseMII CTCAG 6 cut(s) 87, 250, 531, 831, 888, 974
BseNI ACTGG 5 cut(s) 103, 179, 426, 794, 1088
BseRI GAGGAG 1 cut(s) 557
BseXI GCAGC 5 cut(s) 283, 373, 466, 643, 858
BseYI CCCAGC 1 cut(s) 951
BshFI GGCC 4 cut(s) 70, 153, 496, 895
BsiHKAI GWGCWC 1 cut(s) 192
BsiHKCI CYCGRG 1 cut(s) 443
BsiSI CCGG 1 cut(s) 766
BslI CCNNNNNNNGG 4 cut(s) 99, 174, 229, 633
BsmI GAATGC 1 cut(s) 587
BsnI GGCC 4 cut(s) 70, 153, 496, 895
BsoBI CYCGRG 1 cut(s) 443
Bsp1286I GDGCHC 2 cut(s) 192, 958
Bsp1407I TGTACA 1 cut(s) 348
Bsp143I GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
BspACI CCGC 3 cut(s) 133, 309, 1113
BspANI GGCC 4 cut(s) 70, 153, 496, 895
BspCNI CTCAG 6 cut(s) 86, 249, 532, 832, 889, 975
BspLI GGNNCC 4 cut(s) 599, 625, 651, 957
BspMAI CTGCAG 2 cut(s) 502, 668
BspPI GGATC 3 cut(s) 401, 720, 1010
BsrDI GCAATG 1 cut(s) 126
BsrGI TGTACA 1 cut(s) 348
BsrI ACTGG 5 cut(s) 103, 179, 426, 794, 1088
BssECI CCNNGG 3 cut(s) 267, 890, 907
BssMI GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
BssT1I CCWWGG 1 cut(s) 267
Bst2UI CCWGG 3 cut(s) 493, 628, 891
BstAUI TGTACA 1 cut(s) 348
BstC8I GCNNGC 3 cut(s) 133, 498, 554
BstDEI CTNAG 9 cut(s) 73, 236, 523, 540, 840, 851, 897, 983, 1065
BstDSI CCRYGG 1 cut(s) 907
BstEII GGTNACC 1 cut(s) 262
BstF5I GGATG 2 cut(s) 277, 982
BstKTI GATC 7 cut(s) 280, 366, 396, 728, 936, 1005, 1045
BstMBI GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
BstMWI GCNNNNNNNGC 6 cut(s) 67, 428, 497, 502, 656, 880
BstNI CCWGG 3 cut(s) 493, 628, 891
BstNSI RCATGY 2 cut(s) 208, 379
BstPI GGTNACC 1 cut(s) 262
BstSCI CCNGG 3 cut(s) 491, 626, 889
BstSFI CTRYAG 2 cut(s) 498, 664
BstV1I GCAGC 5 cut(s) 283, 373, 466, 643, 858
BstV2I GAAGAC 1 cut(s) 207
Bsu36I CCTNAGG 2 cut(s) 236, 897
BsuRI GGCC 4 cut(s) 70, 153, 496, 895
BtgI CCRYGG 1 cut(s) 907
BtgZI GCGATG 1 cut(s) 825
BtsCI GGATG 2 cut(s) 277, 982
BtsIMutI CAGTG 4 cut(s) 403, 433, 684, 801
Cac8I GCNNGC 3 cut(s) 133, 498, 554
Cfr13I GGNCC 5 cut(s) 69, 151, 232, 614, 893
Csp6I GTAC 3 cut(s) 349, 761, 1038
CviQI GTAC 3 cut(s) 349, 761, 1038
DdeI CTNAG 9 cut(s) 73, 236, 523, 540, 840, 851, 897, 983, 1065
DpnI GATC 7 cut(s) 279, 365, 395, 727, 935, 1004, 1044
DpnII GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
DraIII CACNNNGTG 1 cut(s) 1109
DrdI GACNNNNNNGTC 1 cut(s) 239
DseDI GACNNNNNNGTC 1 cut(s) 239
Eco130I CCWWGG 1 cut(s) 267
Eco24I GRGCYC 1 cut(s) 958
Eco47I GGWCC 2 cut(s) 232, 614
Eco57I CTGAAG 1 cut(s) 992
Eco81I CCTNAGG 2 cut(s) 236, 897
Eco88I CYCGRG 1 cut(s) 443
Eco91I GGTNACC 1 cut(s) 262
EcoO65I GGTNACC 1 cut(s) 262
EcoRI GAATTC 1 cut(s) 218
EcoRII CCWGG 3 cut(s) 491, 626, 889
EcoT14I CCWWGG 1 cut(s) 267
EcoT38I GRGCYC 1 cut(s) 958
ErhI CCWWGG 1 cut(s) 267
FalI AAGNNNNNCTT 2 cut(s) 930, 962
FauI CCCGC 1 cut(s) 140
FbaI TGATCA 2 cut(s) 363, 933
Fnu4HI GCNGC 7 cut(s) 297, 310, 387, 455, 657, 872, 1113
FokI GGATG 2 cut(s) 284, 989
FriOI GRGCYC 1 cut(s) 958
Fsp4HI GCNGC 7 cut(s) 297, 310, 387, 455, 657, 872, 1113
FspBI CTAG 1 cut(s) 390
GluI GCNGC 7 cut(s) 297, 310, 387, 455, 657, 872, 1113
GsaI CCCAGC 1 cut(s) 955
GsuI CTGGAG 1 cut(s) 701
HaeIII GGCC 4 cut(s) 70, 153, 496, 895
HapII CCGG 1 cut(s) 766
HincII GTYRAC 1 cut(s) 771
HindII GTYRAC 1 cut(s) 771
HinfI GANTC 3 cut(s) 318, 576, 714
HpaII CCGG 1 cut(s) 766
HphI GGTGA 2 cut(s) 26, 724
Hpy166II GTNNAC 5 cut(s) 232, 761, 771, 990, 1103
Hpy188I TCNGA 6 cut(s) 76, 86, 172, 623, 781, 922
Hpy188III TCNNGA 5 cut(s) 50, 443, 509, 718, 1000
Hpy8I GTNNAC 5 cut(s) 232, 761, 771, 990, 1103
HpyAV CCTTC 7 cut(s) 253, 472, 588, 640, 739, 749, 967
HpyCH4V TGCA 5 cut(s) 32, 500, 536, 552, 666
HpyF10VI GCNNNNNNNGC 6 cut(s) 67, 428, 497, 502, 656, 880
HpyF3I CTNAG 9 cut(s) 73, 236, 523, 540, 840, 851, 897, 983, 1065
Ksp22I TGATCA 2 cut(s) 363, 933
Kzo9I GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
LmnI GCTCC 3 cut(s) 58, 655, 961
Lsp1109I GCAGC 5 cut(s) 283, 373, 466, 643, 858
LweI GCATC 2 cut(s) 478, 502
MaeI CTAG 1 cut(s) 390
MaeIII GTNAC 2 cut(s) 262, 914
MalI GATC 7 cut(s) 279, 365, 395, 727, 935, 1004, 1044
MboI GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
MboII GAAGA 4 cut(s) 154, 190, 193, 212
MhlI GDGCHC 2 cut(s) 192, 958
MluCI AATT 2 cut(s) 87, 218
MlyI GAGTC 1 cut(s) 708
MmeI TCCRAC 2 cut(s) 354, 804
MseI TTAA 2 cut(s) 372, 450
MslI CAYNNNNRTG 1 cut(s) 195
MspA1I CMGCKG 2 cut(s) 457, 1115
MspI CCGG 1 cut(s) 766
MspR9I CCNGG 3 cut(s) 493, 628, 891
Mva1269I GAATGC 1 cut(s) 587
MvaI CCWGG 3 cut(s) 493, 628, 891
MwoI GCNNNNNNNGC 6 cut(s) 67, 428, 497, 502, 656, 880
NdeII GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
NlaIV GGNNCC 4 cut(s) 599, 625, 651, 957
NspI RCATGY 2 cut(s) 208, 379
OliI CACNNNNGTG 1 cut(s) 195
PciI ACATGT 1 cut(s) 204
PctI GAATGC 1 cut(s) 587
PfeI GAWTC 2 cut(s) 318, 576
PflMI CCANNNNNTGG 1 cut(s) 229
PkrI GCNGC 7 cut(s) 298, 311, 388, 456, 658, 873, 1114
PleI GAGTC 1 cut(s) 708
PpsI GAGTC 1 cut(s) 708
PscI ACATGT 1 cut(s) 204
Psp6I CCWGG 3 cut(s) 491, 626, 889
PspEI GGTNACC 1 cut(s) 262
PspFI CCCAGC 1 cut(s) 951
PspGI CCWGG 3 cut(s) 491, 626, 889
PspN4I GGNNCC 4 cut(s) 599, 625, 651, 957
PspPI GGNCC 5 cut(s) 69, 151, 232, 614, 893
PstI CTGCAG 2 cut(s) 502, 668
PvuII CAGCTG 1 cut(s) 457
RsaI GTAC 3 cut(s) 350, 762, 1039
RsaNI GTAC 3 cut(s) 349, 761, 1038
RseI CAYNNNNRTG 1 cut(s) 195
SaqAI TTAA 2 cut(s) 372, 450
SatI GCNGC 7 cut(s) 297, 310, 387, 455, 657, 872, 1113
Sau3AI GATC 7 cut(s) 277, 363, 393, 725, 933, 1002, 1042
Sau96I GGNCC 5 cut(s) 69, 151, 232, 614, 893
SchI GAGTC 1 cut(s) 708
ScrFI CCNGG 3 cut(s) 493, 628, 891
SduI GDGCHC 2 cut(s) 192, 958
SfaNI GCATC 2 cut(s) 478, 502
SfcI CTRYAG 2 cut(s) 498, 664
SinI GGWCC 2 cut(s) 232, 614
SmiMI CAYNNNNRTG 1 cut(s) 195
SmlI CTYRAG 3 cut(s) 50, 507, 1080
SmoI CTYRAG 3 cut(s) 50, 507, 1080
Sse9I AATT 2 cut(s) 87, 218
SsiI CCGC 3 cut(s) 133, 309, 1113
SspI AATATT 1 cut(s) 802
SspMI CTAG 1 cut(s) 390
StyD4I CCNGG 3 cut(s) 491, 626, 889
StyI CCWWGG 1 cut(s) 267
TaqI TCGA 2 cut(s) 276, 579
TasI AATT 2 cut(s) 87, 218
TatI WGTACW 2 cut(s) 348, 760
TauI GCSGC 2 cut(s) 312, 1115
TfiI GAWTC 2 cut(s) 318, 576
Tru1I TTAA 2 cut(s) 372, 450
Tru9I TTAA 2 cut(s) 372, 450
TscAI CASTG 4 cut(s) 403, 433, 691, 801
TseI GCWGC 5 cut(s) 296, 386, 454, 656, 871
TspDTI ATGAA 4 cut(s) 231, 310, 759, 842
TspGWI ACGGA 2 cut(s) 776, 896
TspRI CASTG 4 cut(s) 403, 433, 691, 801
Van91I CCANNNNNTGG 1 cut(s) 229
VpaK11BI GGWCC 2 cut(s) 232, 614
XapI RAATTY 2 cut(s) 87, 218
XceI RCATGY 2 cut(s) 208, 379
XcmI CCANNNNNNNNNTGG 1 cut(s) 608
XspI CTAG 1 cut(s) 390
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.