RLG00000004431

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_laevigata
Chr1
OG0000415
Physical Location & Seq
Forward (+)
59288863 .. 59291499
2637 bp
Loading structure...
UTR
Exon/CDS
Intron
RLM00000004431

Sequence Viewer

Length: 1194 bp
ATGTCCCGGATTAACGACTCTGAGGAAGTGAAGCATGATATTAGTTTGGCAAACGCTTCCGATCCATGGTGTAATACTTGCTTCAGATATAAGGAAGCTGGAGAAACAGGATTTCTGAACCTTGGTGATGGCCGACAACTTTGCTCAGATTGTTCTTCTATAGCGGTCACGAATTTAGAGGAATGCAATTTCATAATCCAAAATGTGTTTGAATTCTACAGAATCTTAAATCTCAAAGTGGATGAAAAAATTCCCATCTTCTTCGTCGACAAGATTCAAATGAGCAGAATATCTCAAGGTGGTGCAGTTGCTATGTTCATTCCAAGTATATCGGCTTACTTTACAACGTCAACGGCTTGCATATCATTACGAGTTAAAATAATGCTTATTTTAGCCAGATGTATTATACGCAGTATTCAAACGATAGAAAAATATTCGAGAAGTGGAGGTGGTATTAAAAAGGTTGTGAAGCTCCCATTAGGAGAGGCAATTCTACTTTTGTTTGGAACACCAGGTATAGAAATGGGAGCAACCTTGGCACATGAGATGATGCATGGATGGTTCCGCCTTCAAGGTATGCCATTGGGATGTGAAAATAGCCCTGAAGAAGGTATTTGCCAAGTAATGTCTTACAAATGGTTGCAGTGGTTTTCTTCCAGTGGTCTTGATACCTCGCACAAAACCAATCAGCAAGTTCAATATACAAGAAAGCTGAAAGAGTTCTTAGTAGAAGAAATTCGGTCTCGAGAAGACGATGTTTATGGCCAAGGATTCAGATATGCTATGCAAGCTGTTGAAACATTTGGCTTTAAAACCACACTAGACCATGCCGTTAAAAATGGAACTCTACCACTACCTATGATGGATAGTACACGCTCCACCGCCACAGCAGCCGCCGTCACCACTACCACCAAGAAGTTAATAAGAAAGAATACCAGTGCCACCCCAATCACTCGGTTATTATGGAAGGCAACCAGCAATACAACCAAATCACTATGGAAGGCCATAGCTGGCGCAATAACTGACTTATTATGGAAGGCGGCCACCACCTACGAAACGCCGCGGTTTATTATTATGGAAGGTCACTGCCACAATCAATATCATCAAGGGCAAAGCCATAAAAACTTACAAGAAGGCATGTCCTTGTCCCCTATGCTCCTTGTATTTATTGGTATACTAATTGAACACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

398

Amino Acids

44.58

Weight (kDa)

8.71

Isoelectric Point (pI)

42.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 76 - 277 2.6e-34 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 2 cut(s) 267, 1174
AccII CGCG 1 cut(s) 1063
AciI CCGC 7 cut(s) 164, 565, 882, 894, 1040, 1061, 1063
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 3 cut(s) 130, 763, 1041
AcsI RAATTY 4 cut(s) 172, 212, 249, 735
AcuI CTGAAG 2 cut(s) 67, 624
AfaI GTAC 1 cut(s) 871
AfiI CCNNNNNNNGG 2 cut(s) 66, 608
AgsI TTSAA 7 cut(s) 212, 278, 419, 572, 698, 797, 1184
AjnI CCWGG 1 cut(s) 511
AluBI AGCT 5 cut(s) 98, 472, 712, 791, 1010
AluI AGCT 5 cut(s) 98, 472, 712, 791, 1010
Alw26I GTCTC 1 cut(s) 747
AlwI GGATC 1 cut(s) 56
Ama87I CYCGRG 1 cut(s) 744
AoxI GGCC 4 cut(s) 130, 763, 1002, 1041
ApeKI GCWGC 1 cut(s) 890
ApoI RAATTY 4 cut(s) 172, 212, 249, 735
Asp700I GAANNNNTTC 3 cut(s) 249, 719, 735
AspLEI GCGC 1 cut(s) 1016
AsuC2I CCSGG 1 cut(s) 7
AsuHPI GGTGA 2 cut(s) 137, 892
AvaI CYCGRG 1 cut(s) 744
BalI TGGCCA 1 cut(s) 765
BbsI GAAGAC 1 cut(s) 756
BbvI GCAGC 1 cut(s) 902
BccI CCATC 4 cut(s) 122, 263, 552, 856
BceAI ACGGC 3 cut(s) 369, 815, 881
BcgI CGANNNNNNTGC 2 cut(s) 123, 157
BciT130I CCWGG 1 cut(s) 513
BcnI CCSGG 1 cut(s) 7
BcoDI GTCTC 1 cut(s) 747
BfaI CTAG 1 cut(s) 821
BfmI CTRYAG 2 cut(s) 159, 217
BisI GCNGC 4 cut(s) 891, 894, 1041, 1061
BlsI GCNGC 4 cut(s) 892, 895, 1042, 1062
Bme1390I CCNGG 2 cut(s) 7, 513
BmeT110I CYCGRG 1 cut(s) 744
BmiI GGNNCC 1 cut(s) 563
BmrFI CCNGG 2 cut(s) 7, 513
BmsI GCATC 1 cut(s) 540
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 279
BpuMI CCSGG 1 cut(s) 7
BsaI GGTCTC 1 cut(s) 747
BsaJI CCNNGG 5 cut(s) 65, 121, 534, 766, 1061
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 608
Bse1I ACTGG 2 cut(s) 657, 936
BseBI CCWGG 1 cut(s) 513
BseDI CCNNGG 5 cut(s) 65, 121, 534, 766, 1061
BseGI GGATG 3 cut(s) 247, 563, 593
BseLI CCNNNNNNNGG 2 cut(s) 66, 608
BseMII CTCAG 2 cut(s) 12, 159
BseNI ACTGG 2 cut(s) 657, 936
BseXI GCAGC 1 cut(s) 902
BsgI GTGCAG 1 cut(s) 324
Bsh1236I CGCG 1 cut(s) 1063
BshFI GGCC 4 cut(s) 132, 765, 1004, 1043
BsiHKCI CYCGRG 1 cut(s) 744
BsiSI CCGG 1 cut(s) 7
BslFI GGGAC 1 cut(s) 1132
BslI CCNNNNNNNGG 2 cut(s) 66, 608
BsmAI GTCTC 1 cut(s) 747
BsmFI GGGAC 1 cut(s) 1132
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 4 cut(s) 132, 765, 1004, 1043
Bso31I GGTCTC 1 cut(s) 747
BsoBI CYCGRG 1 cut(s) 744
Bsp143I GATC 1 cut(s) 61
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 7 cut(s) 164, 565, 882, 894, 1040, 1061, 1063
BspANI GGCC 4 cut(s) 132, 765, 1004, 1043
BspCNI CTCAG 2 cut(s) 13, 158
BspFNI CGCG 1 cut(s) 1063
BspLI GGNNCC 1 cut(s) 563
BspPI GGATC 1 cut(s) 56
BspTNI GGTCTC 1 cut(s) 747
BsrI ACTGG 2 cut(s) 657, 936
BssECI CCNNGG 5 cut(s) 65, 121, 534, 766, 1061
BssMI GATC 1 cut(s) 61
BssNAI GTATAC 1 cut(s) 1175
BssT1I CCWWGG 4 cut(s) 65, 121, 534, 766
Bst1107I GTATAC 1 cut(s) 1175
Bst2UI CCWGG 1 cut(s) 513
BstC8I GCNNGC 3 cut(s) 358, 789, 1012
BstDEI CTNAG 3 cut(s) 21, 145, 724
BstDSI CCRYGG 2 cut(s) 65, 1061
BstENI CCTNNNNNAGG 1 cut(s) 606
BstF5I GGATG 3 cut(s) 247, 563, 593
BstFNI CGCG 1 cut(s) 1063
BstHHI GCGC 1 cut(s) 1016
BstKTI GATC 1 cut(s) 64
BstMAI GTCTC 1 cut(s) 747
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 3 cut(s) 536, 788, 890
BstNI CCWGG 1 cut(s) 513
BstNSI RCATGY 1 cut(s) 1141
BstSCI CCNGG 2 cut(s) 5, 511
BstSFI CTRYAG 2 cut(s) 159, 217
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 1 cut(s) 902
BstV2I GAAGAC 1 cut(s) 756
BstZ17I GTATAC 1 cut(s) 1175
BsuRI GGCC 4 cut(s) 132, 765, 1004, 1043
BtgI CCRYGG 2 cut(s) 65, 1061
BtsCI GGATG 3 cut(s) 247, 563, 593
BtsI GCAGTG 2 cut(s) 650, 1084
BtsIMutI CAGTG 4 cut(s) 650, 664, 943, 1084
Cac8I GCNNGC 3 cut(s) 358, 789, 1012
CfoI GCGC 1 cut(s) 1016
Cfr42I CCGCGG 1 cut(s) 1064
CsiI ACCWGGT 1 cut(s) 511
Csp6I GTAC 1 cut(s) 870
CviAII CATG 6 cut(s) 35, 66, 542, 554, 827, 1138
CviQI GTAC 1 cut(s) 870
DdeI CTNAG 3 cut(s) 21, 145, 724
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
DraI TTTAAA 1 cut(s) 811
EaeI YGGCCR 3 cut(s) 130, 763, 1041
EciI GGCGGA 1 cut(s) 554
Eco130I CCWWGG 4 cut(s) 65, 121, 534, 766
Eco31I GGTCTC 1 cut(s) 747
Eco57I CTGAAG 2 cut(s) 67, 624
Eco88I CYCGRG 1 cut(s) 744
EcoNI CCTNNNNNAGG 1 cut(s) 606
EcoRI GAATTC 1 cut(s) 212
EcoRII CCWGG 1 cut(s) 511
EcoT14I CCWWGG 4 cut(s) 65, 121, 534, 766
EcoT22I ATGCAT 1 cut(s) 555
ErhI CCWWGG 4 cut(s) 65, 121, 534, 766
FaeI CATG 6 cut(s) 38, 69, 545, 557, 830, 1141
FaqI GGGAC 1 cut(s) 1132
FatI CATG 6 cut(s) 34, 65, 541, 553, 826, 1137
FblI GTMKAC 2 cut(s) 267, 1174
Fnu4HI GCNGC 4 cut(s) 891, 894, 1041, 1061
FokI GGATG 3 cut(s) 254, 570, 600
Fsp4HI GCNGC 4 cut(s) 891, 894, 1041, 1061
FspBI CTAG 1 cut(s) 821
GlaI GCGC 1 cut(s) 1015
GluI GCNGC 4 cut(s) 891, 894, 1041, 1061
GsuI CTGGAG 1 cut(s) 120
HaeIII GGCC 4 cut(s) 132, 765, 1004, 1043
HapII CCGG 1 cut(s) 7
HhaI GCGC 1 cut(s) 1016
Hin1II CATG 6 cut(s) 38, 69, 545, 557, 830, 1141
Hin6I GCGC 1 cut(s) 1014
HinP1I GCGC 1 cut(s) 1014
HincII GTYRAC 2 cut(s) 268, 351
HindII GTYRAC 2 cut(s) 268, 351
HinfI GANTC 4 cut(s) 17, 222, 274, 771
HpaII CCGG 1 cut(s) 7
HphI GGTGA 2 cut(s) 137, 892
Hpy166II GTNNAC 4 cut(s) 268, 351, 872, 1175
Hpy188I TCNGA 6 cut(s) 22, 61, 86, 117, 148, 776
Hpy188III TCNNGA 5 cut(s) 169, 438, 665, 744, 746
Hpy8I GTNNAC 4 cut(s) 268, 351, 872, 1175
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 7 cut(s) 578, 602, 961, 994, 1030, 1073, 1127
HpyCH4IV ACGT 1 cut(s) 347
HpyCH4V TGCA 6 cut(s) 186, 305, 360, 553, 643, 787
HpyF10VI GCNNNNNNNGC 3 cut(s) 536, 788, 890
HpyF3I CTNAG 3 cut(s) 21, 145, 724
HpySE526I ACGT 1 cut(s) 347
Hsp92II CATG 6 cut(s) 38, 69, 545, 557, 830, 1141
HspAI GCGC 1 cut(s) 1014
KspI CCGCGG 1 cut(s) 1064
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 4 cut(s) 477, 527, 881, 1161
Lsp1109I GCAGC 1 cut(s) 902
LweI GCATC 1 cut(s) 540
MabI ACCWGGT 1 cut(s) 511
MaeI CTAG 1 cut(s) 821
MaeII ACGT 1 cut(s) 347
MaeIII GTNAC 3 cut(s) 166, 898, 1082
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 7 cut(s) 147, 250, 253, 617, 645, 743, 761
MlsI TGGCCA 1 cut(s) 765
MluCI AATT 7 cut(s) 172, 187, 212, 249, 489, 735, 1179
MluNI TGGCCA 1 cut(s) 765
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 5 cut(s) 16, 172, 440, 478, 682
Mox20I TGGCCA 1 cut(s) 765
Mph1103I ATGCAT 1 cut(s) 555
MroXI GAANNNNTTC 3 cut(s) 249, 719, 735
MscI TGGCCA 1 cut(s) 765
MseI TTAA 7 cut(s) 12, 227, 375, 456, 810, 834, 920
MslI CAYNNNNRTG 1 cut(s) 586
Msp20I TGGCCA 1 cut(s) 765
MspA1I CMGCKG 1 cut(s) 1063
MspI CCGG 1 cut(s) 7
MspR9I CCNGG 2 cut(s) 7, 513
Mva1269I GAATGC 1 cut(s) 188
MvaI CCWGG 1 cut(s) 513
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 3 cut(s) 536, 788, 890
NciI CCSGG 1 cut(s) 7
NcoI CCATGG 1 cut(s) 65
NdeII GATC 1 cut(s) 61
NlaIII CATG 6 cut(s) 38, 69, 545, 557, 830, 1141
NlaIV GGNNCC 1 cut(s) 563
NmuCI GTSAC 3 cut(s) 166, 898, 1082
NsiI ATGCAT 1 cut(s) 555
NspI RCATGY 1 cut(s) 1141
PaeR7I CTCGAG 1 cut(s) 744
PctI GAATGC 1 cut(s) 188
PdmI GAANNNNTTC 3 cut(s) 249, 719, 735
PfeI GAWTC 3 cut(s) 222, 274, 771
PfoI TCCNGGA 1 cut(s) 5
PkrI GCNGC 4 cut(s) 892, 895, 1042, 1062
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
Psp6I CCWGG 1 cut(s) 511
PspGI CCWGG 1 cut(s) 511
PspN4I GGNNCC 1 cut(s) 563
RsaI GTAC 1 cut(s) 871
RsaNI GTAC 1 cut(s) 870
RseI CAYNNNNRTG 1 cut(s) 586
SacII CCGCGG 1 cut(s) 1064
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 7 cut(s) 12, 227, 375, 456, 810, 834, 920
SatI GCNGC 4 cut(s) 891, 894, 1041, 1061
Sau3AI GATC 1 cut(s) 61
SchI GAGTC 1 cut(s) 11
ScrFI CCNGG 2 cut(s) 7, 513
SexAI ACCWGGT 1 cut(s) 511
SfaNI GCATC 1 cut(s) 540
SfcI CTRYAG 2 cut(s) 159, 217
Sfr274I CTCGAG 1 cut(s) 744
Sfr303I CCGCGG 1 cut(s) 1064
SgrBI CCGCGG 1 cut(s) 1064
SlaI CTCGAG 1 cut(s) 744
SmiMI CAYNNNNRTG 1 cut(s) 586
SmlI CTYRAG 2 cut(s) 294, 744
SmoI CTYRAG 2 cut(s) 294, 744
Sse9I AATT 7 cut(s) 172, 187, 212, 249, 489, 735, 1179
SsiI CCGC 7 cut(s) 164, 565, 882, 894, 1040, 1061, 1063
SspI AATATT 1 cut(s) 434
SspMI CTAG 1 cut(s) 821
StyD4I CCNGG 2 cut(s) 5, 511
StyI CCWWGG 4 cut(s) 65, 121, 534, 766
TaiI ACGT 1 cut(s) 350
TaqI TCGA 3 cut(s) 267, 437, 745
TaqII GACCGA 1 cut(s) 729
TasI AATT 7 cut(s) 172, 187, 212, 249, 489, 735, 1179
TatI WGTACW 1 cut(s) 869
TauI GCSGC 3 cut(s) 896, 1043, 1063
TfiI GAWTC 3 cut(s) 222, 274, 771
Tru1I TTAA 7 cut(s) 12, 227, 375, 456, 810, 834, 920
Tru9I TTAA 7 cut(s) 12, 227, 375, 456, 810, 834, 920
TscAI CASTG 4 cut(s) 650, 664, 943, 1091
TseFI GTSAC 3 cut(s) 166, 898, 1082
TseI GCWGC 1 cut(s) 890
Tsp45I GTSAC 3 cut(s) 166, 898, 1082
TspDTI ATGAA 3 cut(s) 181, 258, 307
TspRI CASTG 4 cut(s) 650, 664, 943, 1091
XagI CCTNNNNNAGG 1 cut(s) 606
XapI RAATTY 4 cut(s) 172, 212, 249, 735
XceI RCATGY 1 cut(s) 1141
XhoI CTCGAG 1 cut(s) 744
XmiI GTMKAC 2 cut(s) 267, 1174
XmnI GAANNNNTTC 3 cut(s) 249, 719, 735
XspI CTAG 1 cut(s) 821
Zsp2I ATGCAT 1 cut(s) 555
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.