Rw4G011920

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000415
Physical Location & Seq
Forward (+)
28145062 .. 28151000
5939 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011920.1

Sequence Viewer

Length: 1662 bp
ATGCACCAGCTTTGTTCTCATCCTTGGCTGCTGATGGTGGTTAAGGAGTTACTTGCACATGCAAAGGTCGAATCTGTAAAATCAAAGGAAGTGGCTGAACAGAGGATGCTCCTGAAGAACAGCTGGTTTTCATTTGGATGGCATACGCCTGATGAATCTGATGGTGATACTTTTGAGGGATCACAGTCACCAGAAGAAAGACTGACTGAAGAGGAGTGGCAAGCAATTGATAAGTTACTAAGTTACCAGCCAGATGAAACATTGGCATCTCACTCAGGAAAGGACATGCCGAATATGATCCGGTTTATGGTAACTGTGTCTGTTGATCGGGCTGCTGCTAGGATCGTTGATATAAATCACACTGAGATTTTGTGTTGTAAATTTGAGCAACTTCAAGTGTCTACGAAGTTTAAACATCAAAGTACATACTGTGATGTGTCATGCAGATTCTATGGTCTGTATGCACCGGAAGGCTCGCTTGCTCAGAGCGTATCTAGTGAGCAGAAGGCGAATGCACTGGCTGCTAGCTTTGTATACCATCCAGTTGGAGAGAATGTTGATTGGAGGCTTTCAGCTACCATTTCACCTTGTCATGTCACGGTTTTGATGGAAAGCTGCGATCGCTTTTTGGATTTTGTGAAAAGGAGTAATGCAGTTAGTCCTACTGTTACATTGGAAACGACAACTGCTTTGCAGATGCAAATTGAGAAAGTGACTCATAGAGCACAAGAGCAATTTCAAATGGTATTAGAAGAACAAAGCAGATTTGCCCTTGATATTGATTTGGATGCTCCAAAAGTAAGAATTCCTATCAGAACCAAGGGCTCTTCCAAATGTGATAGCCATTTTCTATTGGATCTTGGTCATTTCACCCTGCAAACGAAGGTCTTTTGTTTTTTGATACTCAGCATGATAAACAGAAGAAGAACATGTATTCTCGTTTTTTTTATAACTGGAAGAGATATTGCTGCATTTTTTATGGATTGTGGTTCCGATTGCCAAATTCGCACTTTGGAATCACCTGATTCTGATAACCACCCACCGGTTACCCCGGAAACTGTTGACAATTTCTATTCTCTCATAGATAGGTGTGGAATGGCTCCACTTGTTGATCAGATCAGAGTACCTCACCCAAGTTACCTGTCAATGCGTATTTCAATTCAAGTGCCAAACCTCCGAGTGCACTTTTCACCATCGAGATTTCAGAGACTCATGAAATTATTAAACATATTTTATGGTACTCTGGAAACTGTTGGTCAGCCAGCTGGGATTGGCAATTCTGTGGCTACATGGCAACCTTGTTTTCTTGTGTTGTCGGGGACAAATATTTGTGTTTTGGAGTCCGAGAAGTCGCAGACTTATCAGCGATACTCCAGCATGGCTGGTGGACAAGTATGTGAGGTTCCTCCTACAAGTATTGGTGGTTCACCATTTGGCCTTGCTGTGATTCACAGGGGGATGGACACTCAAAAGGCTCCCTCTTCAGTTGATGTCTTGGGAGAAACAATTGATCGTGTTGCTGAGATTGGTGAGCCTCCTCAGACCGGCAATTCTAAAACAGCTGACCTTGTCATTAATGGGGCATTGGTGGAGACAAAGTTATCTATTTATGGAAAGACGGGTGATAAAGGTGCTGAAGAACTTGATGAGACTGATGCTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

553

Amino Acids

61.67

Weight (kDa)

5.44

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 165 - 293 3.5e-06 VPS13-like family N-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 950
AccI GTMKAC 2 cut(s) 401, 534
AclWI GGATC 4 cut(s) 187, 292, 350, 864
AcsI RAATTY 3 cut(s) 380, 804, 1002
AcuI CTGAAG 4 cut(s) 134, 228, 1467, 1656
AfaI GTAC 3 cut(s) 424, 1125, 1240
AfiI CCNNNNNNNGG 3 cut(s) 307, 1042, 1544
AflIII ACRYGT 1 cut(s) 929
AgeI ACCGGT 1 cut(s) 1042
AgsI TTSAA 4 cut(s) 395, 740, 1158, 1163
AluBI AGCT 7 cut(s) 10, 123, 528, 575, 615, 1265, 1562
AluI AGCT 7 cut(s) 10, 123, 528, 575, 615, 1265, 1562
Alw21I GWGCWC 2 cut(s) 727, 1185
Alw26I GTCTC 3 cut(s) 1201, 1586, 1643
Alw44I GTGCAC 1 cut(s) 1181
AlwI GGATC 4 cut(s) 187, 292, 350, 864
AoxI GGCC 1 cut(s) 1435
ApaLI GTGCAC 1 cut(s) 1181
ApeKI GCWGC 6 cut(s) 28, 332, 335, 521, 615, 968
ApoI RAATTY 3 cut(s) 380, 804, 1002
ArsI GACNNNNNNTTYG 2 cut(s) 673, 705
AseI ATTAAT 1 cut(s) 1575
AsiGI ACCGGT 1 cut(s) 1042
AsiSI GCGATCGC 1 cut(s) 622
AsuC2I CCSGG 1 cut(s) 1052
AsuNHI GCTAGC 1 cut(s) 524
BaeGI GKGCMC 1 cut(s) 1185
BanII GRGCYC 1 cut(s) 827
Bbv12I GWGCWC 2 cut(s) 727, 1185
BbvI GCAGC 6 cut(s) 15, 319, 322, 508, 602, 955
BccI CCATC 7 cut(s) 28, 132, 155, 546, 601, 1201, 1453
BclI TGATCA 1 cut(s) 1111
BcnI CCSGG 1 cut(s) 1052
BcoDI GTCTC 3 cut(s) 1201, 1586, 1643
BfaI CTAG 3 cut(s) 339, 495, 525
BisI GCNGC 6 cut(s) 29, 333, 336, 522, 616, 969
BlsI GCNGC 6 cut(s) 30, 334, 337, 523, 617, 970
Bme1390I CCNGG 1 cut(s) 1052
BmiI GGNNCC 4 cut(s) 991, 1101, 1404, 1476
BmrFI CCNGG 1 cut(s) 1052
BmsI GCATC 5 cut(s) 96, 275, 687, 778, 1645
BmtI GCTAGC 1 cut(s) 528
BpmI CTGGAG 1 cut(s) 1357
BpuMI CCSGG 1 cut(s) 1052
BsaBI GATNNNNATC 1 cut(s) 354
BsaJI CCNNGG 3 cut(s) 23, 819, 1050
BsaWI WCCGGW 3 cut(s) 300, 466, 1042
BsaXI ACNNNNNCTCC 4 cut(s) 540, 570, 1583, 1613
Bsc4I CCNNNNNNNGG 3 cut(s) 307, 1042, 1544
Bse118I RCCGGY 2 cut(s) 1042, 1544
Bse1I ACTGG 3 cut(s) 522, 542, 958
Bse8I GATNNNNATC 1 cut(s) 354
BseDI CCNNGG 3 cut(s) 23, 819, 1050
BseGI GGATG 6 cut(s) 19, 111, 143, 538, 793, 1464
BseJI GATNNNNATC 1 cut(s) 354
BseLI CCNNNNNNNGG 3 cut(s) 307, 1042, 1544
BseMII CTCAG 6 cut(s) 288, 354, 497, 919, 1512, 1553
BseNI ACTGG 3 cut(s) 522, 542, 958
BseRI GAGGAG 2 cut(s) 227, 1527
BseSI GKGCMC 1 cut(s) 1185
BseXI GCAGC 6 cut(s) 15, 319, 322, 508, 602, 955
BseYI CCCAGC 1 cut(s) 1265
Bsh1285I CGRYCG 1 cut(s) 622
BshFI GGCC 1 cut(s) 1437
BshTI ACCGGT 1 cut(s) 1042
BsiEI CGRYCG 1 cut(s) 622
BsiHKAI GWGCWC 2 cut(s) 727, 1185
BsiSI CCGG 5 cut(s) 301, 467, 1043, 1052, 1545
BslFI GGGAC 1 cut(s) 1333
BslI CCNNNNNNNGG 3 cut(s) 307, 1042, 1544
BsmAI GTCTC 3 cut(s) 1201, 1586, 1643
BsmFI GGGAC 1 cut(s) 1333
BsmI GAATGC 1 cut(s) 517
BsnI GGCC 1 cut(s) 1437
Bsp1286I GDGCHC 3 cut(s) 727, 827, 1185
Bsp143I GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
BspANI GGCC 1 cut(s) 1437
BspCNI CTCAG 6 cut(s) 287, 355, 496, 918, 1513, 1552
BspHI TCATGA 1 cut(s) 1212
BspLI GGNNCC 4 cut(s) 991, 1101, 1404, 1476
BspOI GCTAGC 1 cut(s) 528
BspPI GGATC 4 cut(s) 187, 292, 350, 864
BspQI GCTCTTC 1 cut(s) 832
BsrFI RCCGGY 2 cut(s) 1042, 1544
BsrI ACTGG 3 cut(s) 522, 542, 958
BssAI RCCGGY 2 cut(s) 1042, 1544
BssECI CCNNGG 3 cut(s) 23, 819, 1050
BssMI GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
BssNAI GTATAC 1 cut(s) 535
BssT1I CCWWGG 2 cut(s) 23, 819
Bst1107I GTATAC 1 cut(s) 535
Bst4CI ACNGT 7 cut(s) 186, 316, 431, 601, 667, 1060, 1252
Bst6I CTCTTC 4 cut(s) 204, 832, 952, 1486
BstAPI GCANNNNNTGC 1 cut(s) 521
BstC8I GCNNGC 5 cut(s) 222, 476, 480, 526, 1263
BstDEI CTNAG 7 cut(s) 239, 274, 363, 483, 905, 1521, 1539
BstEII GGTNACC 1 cut(s) 1045
BstF5I GGATG 6 cut(s) 19, 111, 143, 538, 793, 1464
BstKTI GATC 9 cut(s) 182, 300, 328, 345, 622, 859, 1114, 1119, 1513
BstMAI GTCTC 3 cut(s) 1201, 1586, 1643
BstMBI GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
BstMCI CGRYCG 1 cut(s) 622
BstMWI GCNNNNNNNGC 3 cut(s) 521, 621, 1005
BstNSI RCATGY 3 cut(s) 62, 289, 933
BstPI GGTNACC 1 cut(s) 1045
BstSCI CCNGG 1 cut(s) 1050
BstSLI GKGCMC 1 cut(s) 1185
BstV1I GCAGC 6 cut(s) 15, 319, 322, 508, 602, 955
BstX2I RGATCY 1 cut(s) 856
BstXI CCANNNNNNTGG 1 cut(s) 545
BstYI RGATCY 1 cut(s) 856
BstZ17I GTATAC 1 cut(s) 535
BsuRI GGCC 1 cut(s) 1437
BtsCI GGATG 6 cut(s) 19, 111, 143, 538, 793, 1464
BtsIMutI CAGTG 2 cut(s) 360, 515
Cac8I GCNNGC 5 cut(s) 222, 476, 480, 526, 1263
CciI TCATGA 1 cut(s) 1212
Cfr10I RCCGGY 2 cut(s) 1042, 1544
Csp6I GTAC 3 cut(s) 423, 1124, 1239
CspAI ACCGGT 1 cut(s) 1042
CspCI CAANNNNNGTGG 2 cut(s) 72, 107
CviAII CATG 9 cut(s) 59, 286, 441, 593, 910, 930, 1213, 1290, 1378
CviQI GTAC 3 cut(s) 423, 1124, 1239
DdeI CTNAG 7 cut(s) 239, 274, 363, 483, 905, 1521, 1539
DpnI GATC 9 cut(s) 181, 299, 327, 344, 621, 858, 1113, 1118, 1512
DpnII GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
DraI TTTAAA 1 cut(s) 412
Eam1104I CTCTTC 4 cut(s) 204, 832, 952, 1486
EarI CTCTTC 4 cut(s) 204, 832, 952, 1486
Eco130I CCWWGG 2 cut(s) 23, 819
Eco24I GRGCYC 1 cut(s) 827
Eco57I CTGAAG 4 cut(s) 134, 228, 1467, 1656
Eco91I GGTNACC 1 cut(s) 1045
EcoO65I GGTNACC 1 cut(s) 1045
EcoRI GAATTC 1 cut(s) 804
EcoT14I CCWWGG 2 cut(s) 23, 819
EcoT38I GRGCYC 1 cut(s) 827
ErhI CCWWGG 2 cut(s) 23, 819
FaeI CATG 9 cut(s) 62, 289, 444, 596, 913, 933, 1216, 1293, 1381
FalI AAGNNNNNCTT 2 cut(s) 462, 494
FaqI GGGAC 1 cut(s) 1333
FatI CATG 9 cut(s) 58, 285, 440, 592, 909, 929, 1212, 1289, 1377
FbaI TGATCA 1 cut(s) 1111
FblI GTMKAC 2 cut(s) 401, 534
Fnu4HI GCNGC 6 cut(s) 29, 333, 336, 522, 616, 969
FokI GGATG 6 cut(s) 6, 118, 150, 525, 800, 1471
FriOI GRGCYC 1 cut(s) 827
Fsp4HI GCNGC 6 cut(s) 29, 333, 336, 522, 616, 969
FspBI CTAG 3 cut(s) 339, 495, 525
GluI GCNGC 6 cut(s) 29, 333, 336, 522, 616, 969
GsaI CCCAGC 1 cut(s) 1269
GsuI CTGGAG 1 cut(s) 1357
HaeIII GGCC 1 cut(s) 1437
HapII CCGG 5 cut(s) 301, 467, 1043, 1052, 1545
Hin1II CATG 9 cut(s) 62, 289, 444, 596, 913, 933, 1216, 1293, 1381
HincII GTYRAC 1 cut(s) 1063
HindII GTYRAC 1 cut(s) 1063
HinfI GANTC 9 cut(s) 71, 155, 447, 715, 1016, 1025, 1209, 1340, 1447
HpaII CCGG 5 cut(s) 301, 467, 1043, 1052, 1545
Hpy166II GTNNAC 6 cut(s) 402, 535, 1063, 1183, 1388, 1427
Hpy188III TCNNGA 5 cut(s) 112, 276, 1197, 1213, 1244
Hpy8I GTNNAC 6 cut(s) 402, 535, 1063, 1183, 1388, 1427
HpyAV CCTTC 3 cut(s) 464, 499, 877
HpyCH4III ACNGT 7 cut(s) 186, 316, 431, 601, 667, 1060, 1252
HpyF10VI GCNNNNNNNGC 3 cut(s) 521, 621, 1005
HpyF3I CTNAG 7 cut(s) 239, 274, 363, 483, 905, 1521, 1539
Hsp92II CATG 9 cut(s) 62, 289, 444, 596, 913, 933, 1216, 1293, 1381
Ksp22I TGATCA 1 cut(s) 1111
Kzo9I GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
LguI GCTCTTC 1 cut(s) 832
LmnI GCTCC 4 cut(s) 114, 796, 1105, 1480
Lsp1109I GCAGC 6 cut(s) 15, 319, 322, 508, 602, 955
LweI GCATC 5 cut(s) 96, 275, 687, 778, 1645
MaeI CTAG 3 cut(s) 339, 495, 525
MalI GATC 9 cut(s) 181, 299, 327, 344, 621, 858, 1113, 1118, 1512
MboI GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
MfeI CAATTG 2 cut(s) 225, 1506
MflI RGATCY 1 cut(s) 856
MhlI GDGCHC 3 cut(s) 727, 827, 1185
MlyI GAGTC 3 cut(s) 709, 1203, 1349
MmeI TCCRAC 1 cut(s) 526
MseI TTAA 4 cut(s) 42, 411, 1223, 1575
MslI CAYNNNNRTG 1 cut(s) 136
MspA1I CMGCKG 3 cut(s) 123, 1265, 1562
MspI CCGG 5 cut(s) 301, 467, 1043, 1052, 1545
MspR9I CCNGG 1 cut(s) 1052
MssI GTTTAAAC 1 cut(s) 412
MunI CAATTG 2 cut(s) 225, 1506
Mva1269I GAATGC 1 cut(s) 517
MwoI GCNNNNNNNGC 3 cut(s) 521, 621, 1005
NciI CCSGG 1 cut(s) 1052
NdeII GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
NheI GCTAGC 1 cut(s) 524
NlaIII CATG 9 cut(s) 62, 289, 444, 596, 913, 933, 1216, 1293, 1381
NlaIV GGNNCC 4 cut(s) 991, 1101, 1404, 1476
NmuCI GTSAC 3 cut(s) 186, 595, 712
NspI RCATGY 3 cut(s) 62, 289, 933
PagI TCATGA 1 cut(s) 1212
PciI ACATGT 1 cut(s) 929
PciSI GCTCTTC 1 cut(s) 832
PctI GAATGC 1 cut(s) 517
PfeI GAWTC 6 cut(s) 71, 155, 447, 1016, 1025, 1447
PflFI GACNNNGTC 1 cut(s) 1568
PinAI ACCGGT 1 cut(s) 1042
PkrI GCNGC 6 cut(s) 30, 334, 337, 523, 617, 970
Ple19I CGATCG 1 cut(s) 622
PleI GAGTC 3 cut(s) 709, 1203, 1348
PmeI GTTTAAAC 1 cut(s) 412
PpsI GAGTC 3 cut(s) 709, 1203, 1348
PscI ACATGT 1 cut(s) 929
PshBI ATTAAT 1 cut(s) 1575
PsiI TTATAA 1 cut(s) 950
PspEI GGTNACC 1 cut(s) 1045
PspFI CCCAGC 1 cut(s) 1265
PspN4I GGNNCC 4 cut(s) 991, 1101, 1404, 1476
PsrI GAACNNNNNNTAC 4 cut(s) 1386, 1408, 1418, 1440
PsuI RGATCY 1 cut(s) 856
PsyI GACNNNGTC 1 cut(s) 1568
PvuI CGATCG 1 cut(s) 622
PvuII CAGCTG 3 cut(s) 123, 1265, 1562
RgaI GCGATCGC 1 cut(s) 622
RsaI GTAC 3 cut(s) 424, 1125, 1240
RsaNI GTAC 3 cut(s) 423, 1124, 1239
RseI CAYNNNNRTG 1 cut(s) 136
SapI GCTCTTC 1 cut(s) 832
SaqAI TTAA 4 cut(s) 42, 411, 1223, 1575
SatI GCNGC 6 cut(s) 29, 333, 336, 522, 616, 969
Sau3AI GATC 9 cut(s) 179, 297, 325, 342, 619, 856, 1111, 1116, 1510
SchI GAGTC 3 cut(s) 709, 1203, 1349
ScrFI CCNGG 1 cut(s) 1052
SduI GDGCHC 3 cut(s) 727, 827, 1185
SfaAI GCGATCGC 1 cut(s) 622
SfaNI GCATC 5 cut(s) 96, 275, 687, 778, 1645
SgfI GCGATCGC 1 cut(s) 622
SmiMI CAYNNNNRTG 1 cut(s) 136
SspI AATATT 1 cut(s) 1327
SspMI CTAG 3 cut(s) 339, 495, 525
StyD4I CCNGG 1 cut(s) 1050
StyI CCWWGG 2 cut(s) 23, 819
TaaI ACNGT 7 cut(s) 186, 316, 431, 601, 667, 1060, 1252
TaqI TCGA 2 cut(s) 69, 1196
TatI WGTACW 1 cut(s) 422
TfiI GAWTC 6 cut(s) 71, 155, 447, 1016, 1025, 1447
Tru1I TTAA 4 cut(s) 42, 411, 1223, 1575
Tru9I TTAA 4 cut(s) 42, 411, 1223, 1575
TscAI CASTG 2 cut(s) 367, 522
TseFI GTSAC 3 cut(s) 186, 595, 712
TseI GCWGC 6 cut(s) 28, 332, 335, 521, 615, 968
Tsp45I GTSAC 3 cut(s) 186, 595, 712
TspDTI ATGAA 4 cut(s) 120, 168, 270, 1229
TspRI CASTG 2 cut(s) 367, 522
Tth111I GACNNNGTC 1 cut(s) 1568
VneI GTGCAC 1 cut(s) 1181
VspI ATTAAT 1 cut(s) 1575
XapI RAATTY 3 cut(s) 380, 804, 1002
XceI RCATGY 3 cut(s) 62, 289, 933
XmiI GTMKAC 2 cut(s) 401, 534
XspI CTAG 3 cut(s) 339, 495, 525
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.