Rh7DG123500

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7D
OG0000415
Physical Location & Seq
Reverse (-)
10448616 .. 10449233
618 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7DG123500.1

Sequence Viewer

Length: 618 bp
ATGCCATTGGGATGTGAAAATAGGACTGAAGAAGGTATTTGCGAAGTAATGTCTTACAAATGGTTGCAGTGGTTTTCTTCCAGTGGTCTTGATACCTCGCACAAAACCATACAGCAAGTTCAATATACAAGAAAGCTGAAAGAGTTCTTAGTAGAAGAAATTCGGTGTCGAGAAGACGAAGTTTATGGCCAAGGATTCAGAGATGCTATGCATGCTGTTGAAACATTTGGCTTTAAAACCACACTAGACCATGCCGTTAAAAATGGAACTCTACCACTACCTATGATGGATAGTACACGCTCCACTGCCACAGCTGCCGCCGTCACCACTACCACCAAGAAGTTAATAAGAAAGAATACCAGTGCCACCCCAATAACTCGGTTATTATGGAAGGCGACCAGCAATACAACCAAATCATTATGGAAGGCCACAGCTGCCGCAATAACTGACTTATTATGGAAGGCGGCCACCACCTACGAAACGCCGCGGTTTATTATTATGGAAGGTCACTGCCACAACCAATATCATCAAGGGCAAAGCCATAAAAACTTACAAGAAGGCATGTCCTTGTCCCCTATGCTCCTTGTATTTATTGGTATACTAATTGAACACAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

205

Amino Acids

23.27

Weight (kDa)

8.87

Isoelectric Point (pI)

44.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 7 - 85 9.4e-16 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 598
AccII CGCG 1 cut(s) 487
AciI CCGC 5 cut(s) 318, 438, 464, 485, 487
AcoI YGGCCR 2 cut(s) 187, 465
AcsI RAATTY 1 cut(s) 159
AcuI CTGAAG 1 cut(s) 48
AfaI GTAC 1 cut(s) 295
AgsI TTSAA 3 cut(s) 122, 221, 608
AluBI AGCT 3 cut(s) 136, 314, 434
AluI AGCT 3 cut(s) 136, 314, 434
AoxI GGCC 3 cut(s) 187, 426, 465
ApeKI GCWGC 2 cut(s) 314, 434
ApoI RAATTY 1 cut(s) 159
Asp700I GAANNNNTTC 2 cut(s) 143, 159
AsuHPI GGTGA 1 cut(s) 316
BalI TGGCCA 1 cut(s) 189
BbsI GAAGAC 1 cut(s) 180
BbvI GCAGC 2 cut(s) 301, 421
BccI CCATC 1 cut(s) 280
BceAI ACGGC 2 cut(s) 239, 305
BfaI CTAG 1 cut(s) 245
BisI GCNGC 6 cut(s) 315, 318, 435, 438, 465, 485
BlsI GCNGC 6 cut(s) 316, 319, 436, 439, 466, 486
BmsI GCATC 1 cut(s) 193
BpiI GAAGAC 1 cut(s) 180
BsaJI CCNNGG 2 cut(s) 190, 485
Bse1I ACTGG 2 cut(s) 81, 360
BseDI CCNNGG 2 cut(s) 190, 485
BseGI GGATG 1 cut(s) 17
BseNI ACTGG 2 cut(s) 81, 360
BseXI GCAGC 2 cut(s) 301, 421
Bsh1236I CGCG 1 cut(s) 487
BshFI GGCC 3 cut(s) 189, 428, 467
BslFI GGGAC 1 cut(s) 556
BsmFI GGGAC 1 cut(s) 556
BsnI GGCC 3 cut(s) 189, 428, 467
BspACI CCGC 5 cut(s) 318, 438, 464, 485, 487
BspANI GGCC 3 cut(s) 189, 428, 467
BspFNI CGCG 1 cut(s) 487
BsrI ACTGG 2 cut(s) 81, 360
BssECI CCNNGG 2 cut(s) 190, 485
BssNAI GTATAC 1 cut(s) 599
BssT1I CCWWGG 1 cut(s) 190
Bst1107I GTATAC 1 cut(s) 599
BstC8I GCNNGC 1 cut(s) 213
BstDEI CTNAG 1 cut(s) 148
BstDSI CCRYGG 1 cut(s) 485
BstF5I GGATG 1 cut(s) 17
BstFNI CGCG 1 cut(s) 487
BstMWI GCNNNNNNNGC 3 cut(s) 212, 314, 434
BstNSI RCATGY 2 cut(s) 215, 565
BstUI CGCG 1 cut(s) 487
BstV1I GCAGC 2 cut(s) 301, 421
BstV2I GAAGAC 1 cut(s) 180
BstZ17I GTATAC 1 cut(s) 599
BsuRI GGCC 3 cut(s) 189, 428, 467
BtgI CCRYGG 1 cut(s) 485
BtsCI GGATG 1 cut(s) 17
BtsI GCAGTG 3 cut(s) 74, 303, 508
BtsIMutI CAGTG 5 cut(s) 74, 88, 303, 367, 508
Cac8I GCNNGC 1 cut(s) 213
Cfr42I CCGCGG 1 cut(s) 488
Csp6I GTAC 1 cut(s) 294
CviAII CATG 3 cut(s) 212, 251, 562
CviJI RGCY 8 cut(s) 136, 189, 231, 314, 428, 434, 467, 540
CviKI_1 RGCY 8 cut(s) 136, 189, 231, 314, 428, 434, 467, 540
CviQI GTAC 1 cut(s) 294
DdeI CTNAG 1 cut(s) 148
DraI TTTAAA 1 cut(s) 235
EaeI YGGCCR 2 cut(s) 187, 465
Eco130I CCWWGG 1 cut(s) 190
Eco57I CTGAAG 1 cut(s) 48
EcoT14I CCWWGG 1 cut(s) 190
EcoT22I ATGCAT 1 cut(s) 213
ErhI CCWWGG 1 cut(s) 190
FaeI CATG 3 cut(s) 215, 254, 565
FaqI GGGAC 1 cut(s) 556
FatI CATG 3 cut(s) 211, 250, 561
FblI GTMKAC 1 cut(s) 598
Fnu4HI GCNGC 6 cut(s) 315, 318, 435, 438, 465, 485
FokI GGATG 1 cut(s) 24
Fsp4HI GCNGC 6 cut(s) 315, 318, 435, 438, 465, 485
FspBI CTAG 1 cut(s) 245
GluI GCNGC 6 cut(s) 315, 318, 435, 438, 465, 485
HaeIII GGCC 3 cut(s) 189, 428, 467
Hin1II CATG 3 cut(s) 215, 254, 565
HinfI GANTC 1 cut(s) 195
HphI GGTGA 1 cut(s) 316
Hpy166II GTNNAC 2 cut(s) 296, 599
Hpy188I TCNGA 1 cut(s) 200
Hpy188III TCNNGA 2 cut(s) 89, 170
Hpy8I GTNNAC 2 cut(s) 296, 599
HpyAV CCTTC 6 cut(s) 26, 385, 418, 454, 497, 551
HpyCH4V TGCA 2 cut(s) 67, 211
HpyF10VI GCNNNNNNNGC 3 cut(s) 212, 314, 434
HpyF3I CTNAG 1 cut(s) 148
Hsp92II CATG 3 cut(s) 215, 254, 565
KspI CCGCGG 1 cut(s) 488
LmnI GCTCC 2 cut(s) 305, 585
LpnPI CCDG 4 cut(s) 94, 373, 412, 598
Lsp1109I GCAGC 2 cut(s) 301, 421
LweI GCATC 1 cut(s) 193
MaeI CTAG 1 cut(s) 245
MaeIII GTNAC 2 cut(s) 322, 506
MboII GAAGA 4 cut(s) 41, 69, 167, 185
MlsI TGGCCA 1 cut(s) 189
MluCI AATT 2 cut(s) 159, 603
MluNI TGGCCA 1 cut(s) 189
MnlI CCTC 1 cut(s) 106
Mox20I TGGCCA 1 cut(s) 189
Mph1103I ATGCAT 1 cut(s) 213
MroXI GAANNNNTTC 2 cut(s) 143, 159
MscI TGGCCA 1 cut(s) 189
MseI TTAA 3 cut(s) 234, 258, 344
MslI CAYNNNNRTG 1 cut(s) 10
Msp20I TGGCCA 1 cut(s) 189
MspA1I CMGCKG 3 cut(s) 314, 434, 487
MvnI CGCG 1 cut(s) 487
MwoI GCNNNNNNNGC 3 cut(s) 212, 314, 434
NlaIII CATG 3 cut(s) 215, 254, 565
NmuCI GTSAC 2 cut(s) 322, 506
NsiI ATGCAT 1 cut(s) 213
NspI RCATGY 2 cut(s) 215, 565
PaeI GCATGC 1 cut(s) 215
PdmI GAANNNNTTC 2 cut(s) 143, 159
PfeI GAWTC 1 cut(s) 195
PkrI GCNGC 6 cut(s) 316, 319, 436, 439, 466, 486
PvuII CAGCTG 2 cut(s) 314, 434
RsaI GTAC 1 cut(s) 295
RsaNI GTAC 1 cut(s) 294
RseI CAYNNNNRTG 1 cut(s) 10
SacII CCGCGG 1 cut(s) 488
SaqAI TTAA 3 cut(s) 234, 258, 344
SatI GCNGC 6 cut(s) 315, 318, 435, 438, 465, 485
SetI ASST 9 cut(s) 37, 98, 138, 283, 316, 436, 476, 508, 617
SfaNI GCATC 1 cut(s) 193
Sfr303I CCGCGG 1 cut(s) 488
SgrBI CCGCGG 1 cut(s) 488
SmiMI CAYNNNNRTG 1 cut(s) 10
SphI GCATGC 1 cut(s) 215
Sse9I AATT 2 cut(s) 159, 603
SsiI CCGC 5 cut(s) 318, 438, 464, 485, 487
SspMI CTAG 1 cut(s) 245
StyI CCWWGG 1 cut(s) 190
TaqI TCGA 1 cut(s) 169
TasI AATT 2 cut(s) 159, 603
TatI WGTACW 1 cut(s) 293
TauI GCSGC 4 cut(s) 320, 440, 467, 487
TfiI GAWTC 1 cut(s) 195
Tru1I TTAA 3 cut(s) 234, 258, 344
Tru9I TTAA 3 cut(s) 234, 258, 344
TscAI CASTG 5 cut(s) 74, 88, 310, 367, 515
TseFI GTSAC 2 cut(s) 322, 506
TseI GCWGC 2 cut(s) 314, 434
Tsp45I GTSAC 2 cut(s) 322, 506
TspRI CASTG 5 cut(s) 74, 88, 310, 367, 515
XapI RAATTY 1 cut(s) 159
XceI RCATGY 2 cut(s) 215, 565
XmiI GTMKAC 1 cut(s) 598
XmnI GAANNNNTTC 2 cut(s) 143, 159
XspI CTAG 1 cut(s) 245
Zsp2I ATGCAT 1 cut(s) 213
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.