FvH4_6g04540

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000415
Physical Location & Seq
Forward (+)
2508420 .. 2509522
1103 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04540.t1

Sequence Viewer

Length: 807 bp
ATGTTAGTGATCATCATCCATAAACATGTGTTCCTACCTATTTGTATAATATTGATAGGTATACCGTATATGGGAGACTGTAAGGAATTCATAAGGACTGAAGAAGGTTTATGCGAATTCATGGCTCACAAATGGTTGGAGTGGTTTCCTTCAAGTGGTTTTGATAACTCGCACAAAACCAATAAACAAGTTCAATATACAGAAGAGCTGAGAGAGTCTTTAGTAAATAACATTCAGTGTAAAATCGATGACGTTTATGGCCAAGGATTCAAAGATGCTTTGCGAGCTATTGAAACATTTGGCTTTAAACCCACCCTAGATTATTTCGTTAAATATAGAACTCTACCACAACCAATGATGGACAGTACATGCTCCGCCGCCGCATCCACCACCGCAAAAGAGCTGGAATCATTTATATTTGATGGAATTCGGTCTAGAGGATACAAGTTTTGTGTCCGAGTATTCATAGCTGGTATACGAGCTATTGGAACAGTTGGTTTTAGACCTACCTTAGATCATCTCATAACATATAGAAGTCTACCACAACTTATGATGGATAGCACATGCTCCACCAACGCCTCCGCCACCTCCGCCGCTGCCGAGTTAACAAGAAAGAATACCAGTGCCACCCCGCAACCTGATCATGTATTATGCAAGGCGTCCGGTAATACAACTAAATCATATTGGGTTGCCACAACAATTGACTTCATAGGGAAGACGGCCACCATCTGCGAAGCTATAGGGATTATTATGATGAAAGGATACCTCCTATCTATCGGTATTGGTATACAAATTGAACACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

269

Amino Acids

29.94

Weight (kDa)

7.56

Isoelectric Point (pI)

30.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 33 - 109 2.2e-10 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 61, 475, 538, 787
AciI CCGC 8 cut(s) 375, 378, 381, 393, 582, 591, 594, 632
AcoI YGGCCR 2 cut(s) 259, 720
AcsI RAATTY 3 cut(s) 86, 116, 426
AcuI CTGAAG 1 cut(s) 120
AcyI GRCGYC 1 cut(s) 659
AfaI GTAC 1 cut(s) 367
AfiI CCNNNNNNNGG 2 cut(s) 71, 155
AflIII ACRYGT 1 cut(s) 25
AgsI TTSAA 5 cut(s) 153, 194, 271, 293, 797
AluBI AGCT 6 cut(s) 208, 287, 403, 470, 482, 737
AluI AGCT 6 cut(s) 208, 287, 403, 470, 482, 737
Alw26I GTCTC 1 cut(s) 69
AoxI GGCC 2 cut(s) 259, 720
ApeKI GCWGC 1 cut(s) 596
ApoI RAATTY 3 cut(s) 86, 116, 426
BalI TGGCCA 1 cut(s) 261
BbsI GAAGAC 1 cut(s) 722
BbvI GCAGC 1 cut(s) 583
BccI CCATC 4 cut(s) 352, 416, 547, 734
BceAI ACGGC 1 cut(s) 735
BciVI GTATCC 2 cut(s) 434, 755
BclI TGATCA 2 cut(s) 9, 640
BcoDI GTCTC 1 cut(s) 69
BfaI CTAG 2 cut(s) 317, 435
BfmI CTRYAG 1 cut(s) 738
BfuI GTATCC 2 cut(s) 434, 755
BisI GCNGC 4 cut(s) 378, 381, 594, 597
BlsI GCNGC 4 cut(s) 379, 382, 595, 598
BmsI GCATC 2 cut(s) 265, 392
BpiI GAAGAC 1 cut(s) 722
Bsa29I ATCGAT 1 cut(s) 246
BsaBI GATNNNNATC 1 cut(s) 14
BsaHI GRCGYC 1 cut(s) 659
BsaJI CCNNGG 1 cut(s) 262
BsaWI WCCGGW 1 cut(s) 662
Bsc4I CCNNNNNNNGG 2 cut(s) 71, 155
Bse1I ACTGG 1 cut(s) 621
Bse8I GATNNNNATC 1 cut(s) 14
BseCI ATCGAT 1 cut(s) 246
BseDI CCNNGG 1 cut(s) 262
BseGI GGATG 2 cut(s) 15, 383
BseJI GATNNNNATC 1 cut(s) 14
BseLI CCNNNNNNNGG 2 cut(s) 71, 155
BseMII CTCAG 1 cut(s) 200
BseNI ACTGG 1 cut(s) 621
BseXI GCAGC 1 cut(s) 583
BshFI GGCC 2 cut(s) 261, 722
BshVI ATCGAT 1 cut(s) 246
BsiSI CCGG 1 cut(s) 663
BslI CCNNNNNNNGG 2 cut(s) 71, 155
BsmAI GTCTC 1 cut(s) 69
BsnI GGCC 2 cut(s) 261, 722
Bsp143I GATC 3 cut(s) 9, 514, 640
BspACI CCGC 8 cut(s) 375, 378, 381, 393, 582, 591, 594, 632
BspANI GGCC 2 cut(s) 261, 722
BspCNI CTCAG 1 cut(s) 201
BspDI ATCGAT 1 cut(s) 246
BspQI GCTCTTC 1 cut(s) 198
BsrI ACTGG 1 cut(s) 621
BssECI CCNNGG 1 cut(s) 262
BssMI GATC 3 cut(s) 9, 514, 640
BssNAI GTATAC 3 cut(s) 62, 476, 788
BssNI GRCGYC 1 cut(s) 659
BssT1I CCWWGG 1 cut(s) 262
Bst1107I GTATAC 3 cut(s) 62, 476, 788
Bst4CI ACNGT 5 cut(s) 66, 80, 365, 493, 803
Bst6I CTCTTC 1 cut(s) 198
BstACI GRCGYC 1 cut(s) 659
BstC8I GCNNGC 1 cut(s) 285
BstDEI CTNAG 2 cut(s) 209, 511
BstF5I GGATG 2 cut(s) 15, 383
BstKTI GATC 3 cut(s) 12, 517, 643
BstMAI GTCTC 1 cut(s) 69
BstMBI GATC 3 cut(s) 9, 514, 640
BstMWI GCNNNNNNNGC 2 cut(s) 284, 590
BstNSI RCATGY 3 cut(s) 29, 372, 567
BstSFI CTRYAG 1 cut(s) 738
BstV1I GCAGC 1 cut(s) 583
BstV2I GAAGAC 1 cut(s) 722
BstZ17I GTATAC 3 cut(s) 62, 476, 788
Bsu15I ATCGAT 1 cut(s) 246
BsuI GTATCC 2 cut(s) 434, 755
BsuRI GGCC 2 cut(s) 261, 722
BsuTUI ATCGAT 1 cut(s) 246
BtsCI GGATG 2 cut(s) 15, 383
BtsIMutI CAGTG 2 cut(s) 242, 628
Cac8I GCNNGC 1 cut(s) 285
ClaI ATCGAT 1 cut(s) 246
CseI GACGC 1 cut(s) 648
Csp6I GTAC 1 cut(s) 366
CspCI CAANNNNNGTGG 2 cut(s) 682, 717
CviAII CATG 5 cut(s) 26, 121, 369, 564, 644
CviQI GTAC 1 cut(s) 366
DdeI CTNAG 2 cut(s) 209, 511
DpnI GATC 3 cut(s) 11, 516, 642
DpnII GATC 3 cut(s) 9, 514, 640
DraI TTTAAA 1 cut(s) 307
EaeI YGGCCR 2 cut(s) 259, 720
Eam1104I CTCTTC 1 cut(s) 198
EarI CTCTTC 1 cut(s) 198
EciI GGCGGA 3 cut(s) 364, 571, 580
Eco130I CCWWGG 1 cut(s) 262
Eco57I CTGAAG 1 cut(s) 120
EcoRI GAATTC 3 cut(s) 86, 116, 426
EcoT14I CCWWGG 1 cut(s) 262
ErhI CCWWGG 1 cut(s) 262
FaeI CATG 5 cut(s) 29, 124, 372, 567, 647
FatI CATG 5 cut(s) 25, 120, 368, 563, 643
FauI CCCGC 1 cut(s) 639
FbaI TGATCA 2 cut(s) 9, 640
FblI GTMKAC 4 cut(s) 61, 475, 538, 787
Fnu4HI GCNGC 4 cut(s) 378, 381, 594, 597
FokI GGATG 2 cut(s) 2, 370
Fsp4HI GCNGC 4 cut(s) 378, 381, 594, 597
FspBI CTAG 2 cut(s) 317, 435
GluI GCNGC 4 cut(s) 378, 381, 594, 597
HaeIII GGCC 2 cut(s) 261, 722
HapII CCGG 1 cut(s) 663
HgaI GACGC 1 cut(s) 648
Hin1I GRCGYC 1 cut(s) 659
Hin1II CATG 5 cut(s) 29, 124, 372, 567, 647
HincII GTYRAC 1 cut(s) 606
HindII GTYRAC 1 cut(s) 606
HinfI GANTC 3 cut(s) 215, 267, 407
HpaI GTTAAC 1 cut(s) 606
HpaII CCGG 1 cut(s) 663
Hpy166II GTNNAC 5 cut(s) 62, 476, 539, 606, 788
Hpy188I TCNGA 1 cut(s) 458
Hpy188III TCNNGA 1 cut(s) 435
Hpy8I GTNNAC 5 cut(s) 62, 476, 539, 606, 788
HpyAV CCTTC 2 cut(s) 98, 159
HpyCH4III ACNGT 5 cut(s) 66, 80, 365, 493, 803
HpyCH4IV ACGT 1 cut(s) 252
HpyCH4V TGCA 1 cut(s) 654
HpyF10VI GCNNNNNNNGC 2 cut(s) 284, 590
HpyF3I CTNAG 2 cut(s) 209, 511
HpySE526I ACGT 1 cut(s) 252
Hsp92I GRCGYC 1 cut(s) 659
Hsp92II CATG 5 cut(s) 29, 124, 372, 567, 647
Ksp22I TGATCA 2 cut(s) 9, 640
KspAI GTTAAC 1 cut(s) 606
Kzo9I GATC 3 cut(s) 9, 514, 640
LguI GCTCTTC 1 cut(s) 198
LmnI GCTCC 2 cut(s) 377, 572
LpnPI CCDG 5 cut(s) 389, 456, 634, 651, 676
Lsp1109I GCAGC 1 cut(s) 583
LweI GCATC 2 cut(s) 265, 392
MaeI CTAG 2 cut(s) 317, 435
MaeII ACGT 1 cut(s) 252
MalI GATC 3 cut(s) 11, 516, 642
MboI GATC 3 cut(s) 9, 514, 640
MboII GAAGA 3 cut(s) 113, 215, 727
MfeI CAATTG 1 cut(s) 699
MlsI TGGCCA 1 cut(s) 261
MluCI AATT 5 cut(s) 86, 116, 426, 699, 792
MluNI TGGCCA 1 cut(s) 261
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 117
MnlI CCTC 4 cut(s) 431, 589, 598, 776
Mox20I TGGCCA 1 cut(s) 261
MscI TGGCCA 1 cut(s) 261
MseI TTAA 3 cut(s) 306, 330, 605
MslI CAYNNNNRTG 1 cut(s) 24
Msp20I TGGCCA 1 cut(s) 261
MspA1I CMGCKG 1 cut(s) 596
MspI CCGG 1 cut(s) 663
MunI CAATTG 1 cut(s) 699
MwoI GCNNNNNNNGC 2 cut(s) 284, 590
NdeII GATC 3 cut(s) 9, 514, 640
NlaIII CATG 5 cut(s) 29, 124, 372, 567, 647
NmeAIII GCCGAG 1 cut(s) 625
NspI RCATGY 3 cut(s) 29, 372, 567
PciI ACATGT 1 cut(s) 25
PciSI GCTCTTC 1 cut(s) 198
PfeI GAWTC 2 cut(s) 267, 407
PkrI GCNGC 4 cut(s) 379, 382, 595, 598
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
PscI ACATGT 1 cut(s) 25
RsaI GTAC 1 cut(s) 367
RsaNI GTAC 1 cut(s) 366
RseI CAYNNNNRTG 1 cut(s) 24
SapI GCTCTTC 1 cut(s) 198
SaqAI TTAA 3 cut(s) 306, 330, 605
SatI GCNGC 4 cut(s) 378, 381, 594, 597
Sau3AI GATC 3 cut(s) 9, 514, 640
SchI GAGTC 1 cut(s) 224
SfaNI GCATC 2 cut(s) 265, 392
SfcI CTRYAG 1 cut(s) 738
SmiMI CAYNNNNRTG 1 cut(s) 24
Sse9I AATT 5 cut(s) 86, 116, 426, 699, 792
SsiI CCGC 8 cut(s) 375, 378, 381, 393, 582, 591, 594, 632
SspI AATATT 1 cut(s) 51
SspMI CTAG 2 cut(s) 317, 435
StyI CCWWGG 1 cut(s) 262
TaaI ACNGT 5 cut(s) 66, 80, 365, 493, 803
TaiI ACGT 1 cut(s) 255
TaqI TCGA 1 cut(s) 246
TaqII GACCGA 1 cut(s) 420
TasI AATT 5 cut(s) 86, 116, 426, 699, 792
TatI WGTACW 1 cut(s) 365
TauI GCSGC 3 cut(s) 380, 383, 596
TfiI GAWTC 2 cut(s) 267, 407
Tru1I TTAA 3 cut(s) 306, 330, 605
Tru9I TTAA 3 cut(s) 306, 330, 605
TscAI CASTG 2 cut(s) 242, 628
TseI GCWGC 1 cut(s) 596
TspDTI ATGAA 5 cut(s) 79, 109, 454, 697, 770
TspRI CASTG 2 cut(s) 242, 628
XapI RAATTY 3 cut(s) 86, 116, 426
XbaI TCTAGA 1 cut(s) 434
XceI RCATGY 3 cut(s) 29, 372, 567
XmiI GTMKAC 4 cut(s) 61, 475, 538, 787
XspI CTAG 2 cut(s) 317, 435
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.