Rorug02G0479800

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000002
OG0000415
Physical Location & Seq
Reverse (-)
61062809 .. 61065107
2299 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug02G0479800.1

Sequence Viewer

Length: 537 bp
ATGGCTCCTTTCAGGTATCATCAGTACCAGGTGGTGGGGAGAGCTCTCCCAACTGAGAAGGATGAGCATCCCAAGATCTTCCGCATGAAGCTCTGGCACACCAATGAGGTCCGTGCCAAATCCAAGTTCTGGTACTTCTTGAGGAAGTTGAAGAAAGTGAAGAAGAGCAATGGACAAATCTTGGCCATCAATGAGATTTTTGAGAAAAATCCAACTACGATCAACAACTATGGTATTTGGCTGCGATACCAGAGTAGGACTGGTTATCACAACATGTACAAGGAGTACAGAAACACAACTCTGAATGGCGCGGTAGAAGACATGTACACTGAGATGGCATCTCGTCACAGGGTGAGGTCTCCGTGCATCCAGATCATCAAGACAGCAACTATCCCAGCAAAACTCTGCAAGAGGGAGAGCACCAAGCAGTTCCATAACTCCAAGATCAAGTTCCCATTGGTGCTCAGGAAGATTAGGCCCCCATCTAGGAAGCTGAAGACAACTTACAAGGCATCCAGGCCCAACATCTTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

178

Amino Acids

21.34

Weight (kDa)

10.45

Isoelectric Point (pI)

42.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Ribosomal_L18A PF01775 7 - 128 6.8e-23 Ribosomal proteins 50S-L18Ae/60S-L20/60S-L18A
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 2 cut(s) 34, 129
AccII CGCG 1 cut(s) 311
AciI CCGC 2 cut(s) 82, 311
AcoI YGGCCR 1 cut(s) 183
AcuI CTGAAG 1 cut(s) 515
AdeI CACNNNGTG 1 cut(s) 352
AfaI GTAC 5 cut(s) 26, 134, 278, 287, 326
AfiI CCNNNNNNNGG 3 cut(s) 34, 129, 486
AflIII ACRYGT 2 cut(s) 273, 321
AgsI TTSAA 1 cut(s) 151
AjnI CCWGG 2 cut(s) 27, 515
AjuI GAANNNNNNNTTGG 4 cut(s) 110, 142, 434, 466
AluBI AGCT 3 cut(s) 44, 91, 493
AluI AGCT 3 cut(s) 44, 91, 493
Alw21I GWGCWC 3 cut(s) 46, 422, 465
Alw26I GTCTC 1 cut(s) 363
AoxI GGCC 3 cut(s) 183, 476, 518
ApeKI GCWGC 1 cut(s) 241
AspLEI GCGC 1 cut(s) 311
AspS9I GGNCC 3 cut(s) 109, 477, 519
AsuHPI GGTGA 1 cut(s) 364
AvaII GGWCC 1 cut(s) 109
BaeI ACNNNNGTAYC 2 cut(s) 238, 271
BalI TGGCCA 1 cut(s) 185
BanII GRGCYC 1 cut(s) 46
BarI GAAGNNNNNNTAC 2 cut(s) 488, 520
BbsI GAAGAC 2 cut(s) 324, 503
Bbv12I GWGCWC 3 cut(s) 46, 422, 465
BbvI GCAGC 1 cut(s) 228
BccI CCATC 3 cut(s) 194, 328, 490
BciT130I CCWGG 2 cut(s) 29, 517
BcoDI GTCTC 1 cut(s) 363
BfaI CTAG 1 cut(s) 486
BglII AGATCT 1 cut(s) 75
BisI GCNGC 1 cut(s) 242
BlsI GCNGC 1 cut(s) 243
Bme1390I CCNGG 2 cut(s) 29, 517
Bme18I GGWCC 1 cut(s) 109
BmgT120I GGNCC 3 cut(s) 109, 477, 519
BmiI GGNNCC 2 cut(s) 6, 479
BmrFI CCNGG 2 cut(s) 29, 517
BmsI GCATC 4 cut(s) 76, 347, 375, 521
BpiI GAAGAC 2 cut(s) 324, 503
Bpu10I CCTNAGC 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 160
BsaBI GATNNNNATC 1 cut(s) 66
BsaI GGTCTC 1 cut(s) 363
Bsc4I CCNNNNNNNGG 3 cut(s) 34, 129, 486
Bse1I ACTGG 1 cut(s) 265
Bse3DI GCAATG 1 cut(s) 175
Bse8I GATNNNNATC 1 cut(s) 66
BseBI CCWGG 2 cut(s) 29, 517
BseGI GGATG 4 cut(s) 67, 67, 366, 512
BseJI GATNNNNATC 1 cut(s) 66
BseLI CCNNNNNNNGG 3 cut(s) 34, 129, 486
BseMI GCAATG 1 cut(s) 175
BseMII CTCAG 3 cut(s) 45, 321, 478
BseNI ACTGG 1 cut(s) 265
BseXI GCAGC 1 cut(s) 228
BseYI CCCAGC 1 cut(s) 394
Bsh1236I CGCG 1 cut(s) 311
BshFI GGCC 3 cut(s) 185, 478, 520
BsiHKAI GWGCWC 3 cut(s) 46, 422, 465
BslI CCNNNNNNNGG 3 cut(s) 34, 129, 486
BsmAI GTCTC 1 cut(s) 363
BsnI GGCC 3 cut(s) 185, 478, 520
Bso31I GGTCTC 1 cut(s) 363
Bsp1286I GDGCHC 3 cut(s) 46, 422, 465
Bsp1407I TGTACA 2 cut(s) 276, 324
Bsp143I GATC 4 cut(s) 75, 219, 372, 444
BspACI CCGC 2 cut(s) 82, 311
BspANI GGCC 3 cut(s) 185, 478, 520
BspCNI CTCAG 3 cut(s) 46, 322, 477
BspFNI CGCG 1 cut(s) 311
BspLI GGNNCC 2 cut(s) 6, 479
BspQI GCTCTTC 1 cut(s) 158
BspTNI GGTCTC 1 cut(s) 363
BsrDI GCAATG 1 cut(s) 175
BsrGI TGTACA 2 cut(s) 276, 324
BsrI ACTGG 1 cut(s) 265
BssMI GATC 4 cut(s) 75, 219, 372, 444
Bst2UI CCWGG 2 cut(s) 29, 517
Bst6I CTCTTC 1 cut(s) 158
BstAUI TGTACA 2 cut(s) 276, 324
BstDEI CTNAG 3 cut(s) 54, 330, 464
BstF5I GGATG 4 cut(s) 67, 67, 366, 512
BstFNI CGCG 1 cut(s) 311
BstHHI GCGC 1 cut(s) 311
BstKTI GATC 4 cut(s) 78, 222, 375, 447
BstMAI GTCTC 1 cut(s) 363
BstMBI GATC 4 cut(s) 75, 219, 372, 444
BstNI CCWGG 2 cut(s) 29, 517
BstNSI RCATGY 2 cut(s) 277, 325
BstSCI CCNGG 2 cut(s) 27, 515
BstUI CGCG 1 cut(s) 311
BstV1I GCAGC 1 cut(s) 228
BstV2I GAAGAC 2 cut(s) 324, 503
BstX2I RGATCY 1 cut(s) 75
BstYI RGATCY 1 cut(s) 75
BsuRI GGCC 3 cut(s) 185, 478, 520
BtsCI GGATG 4 cut(s) 67, 67, 366, 512
BtsIMutI CAGTG 1 cut(s) 327
CfoI GCGC 1 cut(s) 311
Cfr13I GGNCC 3 cut(s) 109, 477, 519
CsiI ACCWGGT 1 cut(s) 27
Csp6I GTAC 5 cut(s) 25, 133, 277, 286, 325
CviAII CATG 3 cut(s) 85, 274, 322
CviJI RGCY 8 cut(s) 5, 44, 91, 185, 241, 478, 493, 520
CviKI_1 RGCY 8 cut(s) 5, 44, 91, 185, 241, 478, 493, 520
CviQI GTAC 5 cut(s) 25, 133, 277, 286, 325
DdeI CTNAG 3 cut(s) 54, 330, 464
DpnI GATC 4 cut(s) 77, 221, 374, 446
DpnII GATC 4 cut(s) 75, 219, 372, 444
DraIII CACNNNGTG 1 cut(s) 352
EaeI YGGCCR 1 cut(s) 183
Eam1104I CTCTTC 1 cut(s) 158
EarI CTCTTC 1 cut(s) 158
Ecl136II GAGCTC 1 cut(s) 44
Eco24I GRGCYC 1 cut(s) 46
Eco31I GGTCTC 1 cut(s) 363
Eco47I GGWCC 1 cut(s) 109
Eco53kI GAGCTC 1 cut(s) 44
Eco57I CTGAAG 1 cut(s) 515
EcoICRI GAGCTC 1 cut(s) 44
EcoO109I RGGNCCY 1 cut(s) 477
EcoRII CCWGG 2 cut(s) 27, 515
EcoT38I GRGCYC 1 cut(s) 46
FaeI CATG 3 cut(s) 88, 277, 325
FaiI YATR 6 cut(s) 86, 231, 275, 323, 435, 533
FatI CATG 3 cut(s) 84, 273, 321
Fnu4HI GCNGC 1 cut(s) 242
FokI GGATG 4 cut(s) 54, 74, 353, 499
FriOI GRGCYC 1 cut(s) 46
Fsp4HI GCNGC 1 cut(s) 242
FspBI CTAG 1 cut(s) 486
GlaI GCGC 1 cut(s) 310
GluI GCNGC 1 cut(s) 242
GsaI CCCAGC 1 cut(s) 398
HaeIII GGCC 3 cut(s) 185, 478, 520
HhaI GCGC 1 cut(s) 311
Hin1II CATG 3 cut(s) 88, 277, 325
Hin6I GCGC 1 cut(s) 309
HinP1I GCGC 1 cut(s) 309
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 327
Hpy188I TCNGA 1 cut(s) 303
Hpy188III TCNNGA 4 cut(s) 139, 370, 379, 466
Hpy8I GTNNAC 1 cut(s) 327
HpyAV CCTTC 1 cut(s) 52
HpyCH4V TGCA 2 cut(s) 366, 408
HpyF3I CTNAG 3 cut(s) 54, 330, 464
Hsp92II CATG 3 cut(s) 88, 277, 325
HspAI GCGC 1 cut(s) 309
Kzo9I GATC 4 cut(s) 75, 219, 372, 444
LguI GCTCTTC 1 cut(s) 158
LmnI GCTCC 1 cut(s) 10
Lsp1109I GCAGC 1 cut(s) 228
LweI GCATC 4 cut(s) 76, 347, 375, 521
MabI ACCWGGT 1 cut(s) 27
MaeI CTAG 1 cut(s) 486
MaeIII GTNAC 1 cut(s) 344
MalI GATC 4 cut(s) 77, 221, 374, 446
MboI GATC 4 cut(s) 75, 219, 372, 444
MboII GAAGA 7 cut(s) 70, 163, 172, 175, 329, 481, 508
MflI RGATCY 1 cut(s) 75
MhlI GDGCHC 3 cut(s) 46, 422, 465
MlsI TGGCCA 1 cut(s) 185
MluNI TGGCCA 1 cut(s) 185
MmeI TCCRAC 1 cut(s) 236
MnlI CCTC 4 cut(s) 100, 135, 348, 405
Mox20I TGGCCA 1 cut(s) 185
MscI TGGCCA 1 cut(s) 185
MslI CAYNNNNRTG 3 cut(s) 102, 332, 530
Msp20I TGGCCA 1 cut(s) 185
MspR9I CCNGG 2 cut(s) 29, 517
MvaI CCWGG 2 cut(s) 29, 517
MvnI CGCG 1 cut(s) 311
NdeII GATC 4 cut(s) 75, 219, 372, 444
NlaIII CATG 3 cut(s) 88, 277, 325
NlaIV GGNNCC 2 cut(s) 6, 479
NmuCI GTSAC 1 cut(s) 344
NspI RCATGY 2 cut(s) 277, 325
PciI ACATGT 2 cut(s) 273, 321
PciSI GCTCTTC 1 cut(s) 158
PflMI CCANNNNNTGG 2 cut(s) 34, 129
PkrI GCNGC 1 cut(s) 243
PscI ACATGT 2 cut(s) 273, 321
Psp124BI GAGCTC 1 cut(s) 46
Psp6I CCWGG 2 cut(s) 27, 515
PspFI CCCAGC 1 cut(s) 394
PspGI CCWGG 2 cut(s) 27, 515
PspN4I GGNNCC 2 cut(s) 6, 479
PspPI GGNCC 3 cut(s) 109, 477, 519
PsuI RGATCY 1 cut(s) 75
RsaI GTAC 5 cut(s) 26, 134, 278, 287, 326
RsaNI GTAC 5 cut(s) 25, 133, 277, 286, 325
RseI CAYNNNNRTG 3 cut(s) 102, 332, 530
SacI GAGCTC 1 cut(s) 46
SapI GCTCTTC 1 cut(s) 158
SatI GCNGC 1 cut(s) 242
Sau3AI GATC 4 cut(s) 75, 219, 372, 444
Sau96I GGNCC 3 cut(s) 109, 477, 519
ScrFI CCNGG 2 cut(s) 29, 517
SduI GDGCHC 3 cut(s) 46, 422, 465
SetI ASST 7 cut(s) 17, 33, 46, 93, 111, 359, 495
SexAI ACCWGGT 1 cut(s) 27
SfaNI GCATC 4 cut(s) 76, 347, 375, 521
SinI GGWCC 1 cut(s) 109
SmiMI CAYNNNNRTG 3 cut(s) 102, 332, 530
SmlI CTYRAG 1 cut(s) 139
SmoI CTYRAG 1 cut(s) 139
SsiI CCGC 2 cut(s) 82, 311
SspMI CTAG 1 cut(s) 486
SstI GAGCTC 1 cut(s) 46
StyD4I CCNGG 2 cut(s) 27, 515
TatI WGTACW 3 cut(s) 276, 285, 324
TscAI CASTG 1 cut(s) 334
TseFI GTSAC 1 cut(s) 344
TseI GCWGC 1 cut(s) 241
Tsp45I GTSAC 1 cut(s) 344
TspDTI ATGAA 1 cut(s) 101
TspGWI ACGGA 2 cut(s) 101, 351
TspRI CASTG 1 cut(s) 334
Van91I CCANNNNNTGG 2 cut(s) 34, 129
VpaK11BI GGWCC 1 cut(s) 109
XceI RCATGY 2 cut(s) 277, 325
XcmI CCANNNNNNNNNTGG 1 cut(s) 257
XspI CTAG 1 cut(s) 486
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.