Rh6DG265800

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000415
Physical Location & Seq
Reverse (-)
45725569 .. 45727468
1900 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG265800.1

Sequence Viewer

Length: 663 bp
ATGCAGCTGGACTGGTGCGTCGATCATTCTGGGATCAAAGATGCTGTCGGGAACATTACTCCGATGATTGAATATTTAAAAACTAAAGGAAATATTGAAGTGTTGACGAAAGTCCCAAAACTCTTAGAAGGAGTCACAATTGTAGTTTTGTTCGGAAAACCAAATGTGGAAATCGGCGGGATCTTGGCACATGAGATGATGCATGCATGGTTACGCCTTCGAGGTAAGCCATGGGAATGGGAAAGAAGAGTTGAGGAAGGCATTTGCGAAGTAATGTATTACAAGTGGTTGAAATGGTTTTGTTCTAGTACTGGATTTCATTCTTCTTACAAATCCAAAGATCATGCTCAATACGCAAGAAAGTTGATAAAGTACTTGGCACAAGGGATTGAGACACGGGAAGACGAAGTTTATGGCCAAGGGTTCAGAGATGCTGTGCTAACGGTTACAAAATTCGGCTTCAAAACTACACCGAACTATACCCTTAAAAATGGAACTCTTCCTGTAGCTCCACCGAAAACCGAGCTATTGTGGAAGGCCACCACTAGTGGTGCTACATTGTTATTGAAGGCCACTGCCACCACCATAACTGATTTATTATGGAAGTCCACCGGCCGCTGTTATGGAAGGCCATCGCCACCACAACTAGGGCCAAAGCCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

220

Amino Acids

24.88

Weight (kDa)

9.33

Isoelectric Point (pI)

32.36

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 45 - 157 7.3e-31 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
iTOL Interactive Tree Of Life · Most popular EvolView Flexible annotations MEGA Desktop phylogenetics suite FigTree Desktop tree editor

Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 17
AciI CCGC 2 cut(s) 177, 616
AclWI GGATC 2 cut(s) 41, 188
AcoI YGGCCR 2 cut(s) 415, 613
AcsI RAATTY 1 cut(s) 452
AfaI GTAC 2 cut(s) 310, 374
AfiI CCNNNNNNNGG 1 cut(s) 647
AgsI TTSAA 5 cut(s) 71, 98, 292, 463, 568
AhlI ACTAGT 1 cut(s) 545
AluBI AGCT 3 cut(s) 7, 509, 526
AluI AGCT 3 cut(s) 7, 509, 526
Alw26I GTCTC 1 cut(s) 386
AlwI GGATC 2 cut(s) 41, 188
AoxI GGCC 6 cut(s) 415, 537, 570, 613, 629, 650
ApeKI GCWGC 1 cut(s) 4
ApoI RAATTY 1 cut(s) 452
ArsI GACNNNNNNTTYG 2 cut(s) 30, 62
AspS9I GGNCC 1 cut(s) 650
BalI TGGCCA 1 cut(s) 417
BbsI GAAGAC 1 cut(s) 408
BbvI GCAGC 1 cut(s) 16
BccI CCATC 1 cut(s) 640
BcoDI GTCTC 1 cut(s) 386
BcuI ACTAGT 1 cut(s) 545
BfaI CTAG 3 cut(s) 306, 546, 647
BfmI CTRYAG 1 cut(s) 504
BisI GCNGC 2 cut(s) 5, 616
BlsI GCNGC 2 cut(s) 6, 617
BmcAI AGTACT 2 cut(s) 310, 374
BmgT120I GGNCC 1 cut(s) 650
BmsI GCATC 3 cut(s) 31, 189, 421
BoxI GACNNNNGTC 1 cut(s) 110
BpiI GAAGAC 1 cut(s) 408
BsaJI CCNNGG 2 cut(s) 230, 418
Bsc4I CCNNNNNNNGG 1 cut(s) 647
Bse118I RCCGGY 1 cut(s) 611
Bse1I ACTGG 2 cut(s) 17, 316
BseDI CCNNGG 2 cut(s) 230, 418
BseLI CCNNNNNNNGG 1 cut(s) 647
BseNI ACTGG 2 cut(s) 17, 316
BseX3I CGGCCG 1 cut(s) 613
BseXI GCAGC 1 cut(s) 16
Bsh1285I CGRYCG 1 cut(s) 616
BshFI GGCC 6 cut(s) 417, 539, 572, 615, 631, 652
BsiEI CGRYCG 1 cut(s) 616
BsiSI CCGG 1 cut(s) 612
BslFI GGGAC 1 cut(s) 98
BslI CCNNNNNNNGG 1 cut(s) 647
BsmAI GTCTC 1 cut(s) 386
BsmFI GGGAC 1 cut(s) 98
BsnI GGCC 6 cut(s) 417, 539, 572, 615, 631, 652
Bsp143I GATC 4 cut(s) 22, 33, 180, 340
Bsp19I CCATGG 1 cut(s) 230
BspACI CCGC 2 cut(s) 177, 616
BspANI GGCC 6 cut(s) 417, 539, 572, 615, 631, 652
BspPI GGATC 2 cut(s) 41, 188
BsrFI RCCGGY 1 cut(s) 611
BsrI ACTGG 2 cut(s) 17, 316
BssAI RCCGGY 1 cut(s) 611
BssECI CCNNGG 2 cut(s) 230, 418
BssMI GATC 4 cut(s) 22, 33, 180, 340
BssT1I CCWWGG 2 cut(s) 230, 418
Bst4CI ACNGT 1 cut(s) 445
Bst6I CTCTTC 2 cut(s) 241, 504
BstC8I GCNNGC 1 cut(s) 204
BstDEI CTNAG 1 cut(s) 124
BstDSI CCRYGG 1 cut(s) 230
BstKTI GATC 4 cut(s) 25, 36, 183, 343
BstMAI GTCTC 1 cut(s) 386
BstMBI GATC 4 cut(s) 22, 33, 180, 340
BstMCI CGRYCG 1 cut(s) 616
BstMWI GCNNNNNNNGC 1 cut(s) 353
BstNSI RCATGY 1 cut(s) 206
BstPAI GACNNNNGTC 1 cut(s) 110
BstSFI CTRYAG 1 cut(s) 504
BstV1I GCAGC 1 cut(s) 16
BstV2I GAAGAC 1 cut(s) 408
BstX2I RGATCY 1 cut(s) 180
BstXI CCANNNNNNTGG 1 cut(s) 237
BstYI RGATCY 1 cut(s) 180
BstZI CGGCCG 1 cut(s) 613
BsuRI GGCC 6 cut(s) 417, 539, 572, 615, 631, 652
BtgI CCRYGG 1 cut(s) 230
BtgZI GCGATG 1 cut(s) 618
BtsI GCAGTG 1 cut(s) 573
BtsIMutI CAGTG 1 cut(s) 573
Cac8I GCNNGC 1 cut(s) 204
Cfr10I RCCGGY 1 cut(s) 611
Cfr13I GGNCC 1 cut(s) 650
CseI GACGC 1 cut(s) 7
Csp6I GTAC 2 cut(s) 309, 373
CviAII CATG 5 cut(s) 191, 203, 207, 231, 344
CviQI GTAC 2 cut(s) 309, 373
DdeI CTNAG 1 cut(s) 124
DpnI GATC 4 cut(s) 24, 35, 182, 342
DpnII GATC 4 cut(s) 22, 33, 180, 340
DraI TTTAAA 1 cut(s) 78
DrdI GACNNNNNNGTC 1 cut(s) 17
DseDI GACNNNNNNGTC 1 cut(s) 17
EaeI YGGCCR 2 cut(s) 415, 613
EagI CGGCCG 1 cut(s) 613
Eam1104I CTCTTC 2 cut(s) 241, 504
EarI CTCTTC 2 cut(s) 241, 504
EclXI CGGCCG 1 cut(s) 613
Eco130I CCWWGG 2 cut(s) 230, 418
Eco52I CGGCCG 1 cut(s) 613
EcoT14I CCWWGG 2 cut(s) 230, 418
EcoT22I ATGCAT 2 cut(s) 204, 208
ErhI CCWWGG 2 cut(s) 230, 418
FaeI CATG 5 cut(s) 194, 206, 210, 234, 347
FaqI GGGAC 1 cut(s) 98
FatI CATG 5 cut(s) 190, 202, 206, 230, 343
FauI CCCGC 1 cut(s) 170
Fnu4HI GCNGC 2 cut(s) 5, 616
Fsp4HI GCNGC 2 cut(s) 5, 616
FspBI CTAG 3 cut(s) 306, 546, 647
GluI GCNGC 2 cut(s) 5, 616
HaeIII GGCC 6 cut(s) 417, 539, 572, 615, 631, 652
HapII CCGG 1 cut(s) 612
HgaI GACGC 1 cut(s) 7
Hin1II CATG 5 cut(s) 194, 206, 210, 234, 347
HincII GTYRAC 1 cut(s) 105
HindII GTYRAC 1 cut(s) 105
HinfI GANTC 1 cut(s) 132
HpaII CCGG 1 cut(s) 612
Hpy166II GTNNAC 2 cut(s) 105, 609
Hpy188I TCNGA 3 cut(s) 63, 155, 428
Hpy188III TCNNGA 1 cut(s) 49
Hpy8I GTNNAC 2 cut(s) 105, 609
Hpy99I CGWCG 1 cut(s) 23
HpyAV CCTTC 6 cut(s) 122, 227, 251, 529, 562, 621
HpyCH4III ACNGT 1 cut(s) 445
HpyCH4V TGCA 3 cut(s) 4, 202, 206
HpyF10VI GCNNNNNNNGC 1 cut(s) 353
HpyF3I CTNAG 1 cut(s) 124
Hsp92II CATG 5 cut(s) 194, 206, 210, 234, 347
Kzo9I GATC 4 cut(s) 22, 33, 180, 340
LmnI GCTCC 1 cut(s) 514
LpnPI CCDG 4 cut(s) 15, 297, 516, 625
Lsp1109I GCAGC 1 cut(s) 16
LweI GCATC 3 cut(s) 31, 189, 421
MaeI CTAG 3 cut(s) 306, 546, 647
MaeIII GTNAC 3 cut(s) 133, 210, 445
MalI GATC 4 cut(s) 24, 35, 182, 342
MboI GATC 4 cut(s) 22, 33, 180, 340
MboII GAAGA 4 cut(s) 258, 315, 413, 491
MfeI CAATTG 1 cut(s) 138
MflI RGATCY 1 cut(s) 180
MlsI TGGCCA 1 cut(s) 417
MluCI AATT 2 cut(s) 138, 452
MluNI TGGCCA 1 cut(s) 417
MlyI GAGTC 1 cut(s) 141
MnlI CCTC 2 cut(s) 215, 247
Mox20I TGGCCA 1 cut(s) 417
Mph1103I ATGCAT 2 cut(s) 204, 208
MscI TGGCCA 1 cut(s) 417
MseI TTAA 2 cut(s) 77, 486
MslI CAYNNNNRTG 1 cut(s) 235
Msp20I TGGCCA 1 cut(s) 417
MspA1I CMGCKG 2 cut(s) 7, 618
MspI CCGG 1 cut(s) 612
MunI CAATTG 1 cut(s) 138
MwoI GCNNNNNNNGC 1 cut(s) 353
NcoI CCATGG 1 cut(s) 230
NdeII GATC 4 cut(s) 22, 33, 180, 340
NlaIII CATG 5 cut(s) 194, 206, 210, 234, 347
NmuCI GTSAC 1 cut(s) 133
NsiI ATGCAT 2 cut(s) 204, 208
NspI RCATGY 1 cut(s) 206
PaeI GCATGC 1 cut(s) 206
PkrI GCNGC 2 cut(s) 6, 617
PleI GAGTC 1 cut(s) 140
PpsI GAGTC 1 cut(s) 140
PshAI GACNNNNGTC 1 cut(s) 110
PspPI GGNCC 1 cut(s) 650
PsuI RGATCY 1 cut(s) 180
PvuII CAGCTG 1 cut(s) 7
RsaI GTAC 2 cut(s) 310, 374
RsaNI GTAC 2 cut(s) 309, 373
RseI CAYNNNNRTG 1 cut(s) 235
SaqAI TTAA 2 cut(s) 77, 486
SatI GCNGC 2 cut(s) 5, 616
Sau3AI GATC 4 cut(s) 22, 33, 180, 340
Sau96I GGNCC 1 cut(s) 650
ScaI AGTACT 2 cut(s) 310, 374
SchI GAGTC 1 cut(s) 141
SetI ASST 4 cut(s) 9, 226, 511, 528
SfaNI GCATC 3 cut(s) 31, 189, 421
SfcI CTRYAG 1 cut(s) 504
SmiMI CAYNNNNRTG 1 cut(s) 235
SpeI ACTAGT 1 cut(s) 545
SphI GCATGC 1 cut(s) 206
Sse9I AATT 2 cut(s) 138, 452
SsiI CCGC 2 cut(s) 177, 616
SspI AATATT 2 cut(s) 74, 94
SspMI CTAG 3 cut(s) 306, 546, 647
StyI CCWWGG 2 cut(s) 230, 418
TaaI ACNGT 1 cut(s) 445
TaqI TCGA 2 cut(s) 21, 220
TasI AATT 2 cut(s) 138, 452
TatI WGTACW 2 cut(s) 308, 372
TauI GCSGC 1 cut(s) 618
Tru1I TTAA 2 cut(s) 77, 486
Tru9I TTAA 2 cut(s) 77, 486
TscAI CASTG 1 cut(s) 580
TseFI GTSAC 1 cut(s) 133
TseI GCWGC 1 cut(s) 4
Tsp45I GTSAC 1 cut(s) 133
TspDTI ATGAA 1 cut(s) 308
TspRI CASTG 1 cut(s) 580
XapI RAATTY 1 cut(s) 452
XceI RCATGY 1 cut(s) 206
XspI CTAG 3 cut(s) 306, 546, 647
ZrmI AGTACT 2 cut(s) 310, 374
Zsp2I ATGCAT 2 cut(s) 204, 208
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.