Rh6DG283200

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000415
Physical Location & Seq
Reverse (-)
47725118 .. 47727588
2471 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG283200.1

Sequence Viewer

Length: 924 bp
ATGGCTACTCAGTGTGGTGTCTGCAATGTAAATTCTAATAAATTGAGCTACTATAAATTTTGGGAGGAATCTGTGTGTCGTGAACATGCCTCTGATGGGACTCTCAGGTGTTATACCTGTCACAGATTTAAGAAAGCTCAGGATCCAGAATATTTGGACCTTAATGATAATGGCAGAAAACTTTGCTCATATTGTTCTAGCATTGCTACCTTGGATCCAAAGGAATGCATGCCCCTTATTCAAAATGTGCGTGAATTTTATAAAAGTTTAAACCTCGTGGTGGATGAAACCATTCCTTTTTTGTTGGTCGACAAGGATATGATGTTCAAATTCATACCAGGCAAAAAGACGATTGGTACCATATGGTACAAGGACAGCAGTTTGCGTGCTTGCACAACGATTAACAAATGTTCAACGCGTGAAGGAATTATTAAGGTGGAGAAACACACAGAGAGGTTAACCCGACGTTGCAACTTATTAACCCTGCGACGAAAAAGTTCAGTAAAGGCATTGATAATTTTGTTCGGTTCACCGGGAGTCCGCATGGGTGAAACAATGGCACATGAGATGATGCATGCATGGTTTTACCTTCAAGGTTGTAGAGGGAAATTGGAAAGAAGTGTTGAAGAAGGCATTTGCAGTTTAATGGGATACATGTGGATGGAGTGGTTTTGTACTAGTGGTGTTGACTCCTTTTACAAAACCAGTGTACAAGTTCAATATACAAAAGACTTGAAAGATTATATGGCGCATTGTATGGAACGTAATCAAGACAAAATTTATGGCCAAGGATTTAGAGATGCTATCAAGGCTGTTAGCAAGTTTGGCTTCAACTATACAGTGGACTATATCGTCGAGAACAAATGCCTCCCTTGTGAAATGGATCCAGCTCCTCTGCAGTGCAGAGGGTGTGCAGTTATGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

307

Amino Acids

35.48

Weight (kDa)

8.61

Isoelectric Point (pI)

41.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 90 - 285 5.1e-34 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 261
AasI GACNNNNNNGTC 1 cut(s) 851
Acc65I GGTACC 1 cut(s) 356
AccB1I GGYRCC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 309
AccII CGCG 1 cut(s) 418
AciI CCGC 1 cut(s) 541
AclWI GGATC 6 cut(s) 137, 150, 209, 222, 878, 891
AcoI YGGCCR 1 cut(s) 784
AcsI RAATTY 5 cut(s) 31, 56, 254, 329, 777
AfaI GTAC 4 cut(s) 358, 368, 676, 711
AfiI CCNNNNNNNGG 2 cut(s) 96, 280
AflIII ACRYGT 2 cut(s) 416, 654
AgsI TTSAA 8 cut(s) 242, 328, 414, 593, 626, 719, 736, 832
AhlI ACTAGT 1 cut(s) 677
AjnI CCWGG 1 cut(s) 337
AloI GAACNNNNNNTCC 2 cut(s) 308, 340
AluBI AGCT 3 cut(s) 48, 137, 890
AluI AGCT 3 cut(s) 48, 137, 890
AlwI GGATC 6 cut(s) 137, 150, 209, 222, 878, 891
AoxI GGCC 1 cut(s) 784
ApoI RAATTY 5 cut(s) 31, 56, 254, 329, 777
Asp700I GAANNNNTTC 2 cut(s) 291, 496
Asp718I GGTACC 1 cut(s) 356
AspLEI GCGC 1 cut(s) 751
AspS9I GGNCC 1 cut(s) 157
AsuC2I CCSGG 1 cut(s) 534
AsuHPI GGTGA 2 cut(s) 522, 560
AvaII GGWCC 1 cut(s) 157
BalI TGGCCA 1 cut(s) 786
BamHI GGATCC 3 cut(s) 142, 214, 883
BanI GGYRCC 1 cut(s) 356
BauI CACGAG 1 cut(s) 275
BccI CCATC 2 cut(s) 89, 655
BciT130I CCWGG 1 cut(s) 339
BciVI GTATCC 1 cut(s) 644
BcnI CCSGG 1 cut(s) 534
BcuI ACTAGT 1 cut(s) 677
BfaI CTAG 2 cut(s) 198, 678
BfmI CTRYAG 1 cut(s) 896
BfuI GTATCC 1 cut(s) 644
Bme1390I CCNGG 2 cut(s) 339, 534
Bme18I GGWCC 1 cut(s) 157
BmgT120I GGNCC 1 cut(s) 157
BmiI GGNNCC 4 cut(s) 144, 216, 358, 885
BmrFI CCNGG 2 cut(s) 339, 534
BmsI GCATC 2 cut(s) 561, 790
Bpu10I CCTNAGC 1 cut(s) 138
BpuMI CCSGG 1 cut(s) 534
BsaJI CCNNGG 2 cut(s) 210, 787
Bsc4I CCNNNNNNNGG 2 cut(s) 96, 280
Bse1I ACTGG 1 cut(s) 705
Bse3DI GCAATG 2 cut(s) 31, 201
BseBI CCWGG 1 cut(s) 339
BseDI CCNNGG 2 cut(s) 210, 787
BseGI GGATG 2 cut(s) 289, 666
BseLI CCNNNNNNNGG 2 cut(s) 96, 280
BseMI GCAATG 2 cut(s) 31, 201
BseMII CTCAG 3 cut(s) 23, 118, 152
BseNI ACTGG 1 cut(s) 705
BseRI GAGGAG 1 cut(s) 882
BsgI GTGCAG 1 cut(s) 922
Bsh1236I CGCG 1 cut(s) 418
BshFI GGCC 1 cut(s) 786
BshNI GGYRCC 1 cut(s) 356
BsiSI CCGG 1 cut(s) 533
BslFI GGGAC 1 cut(s) 112
BslI CCNNNNNNNGG 2 cut(s) 96, 280
BsmFI GGGAC 1 cut(s) 112
BsmI GAATGC 1 cut(s) 230
BsnI GGCC 1 cut(s) 786
Bsp1407I TGTACA 1 cut(s) 709
Bsp143I GATC 3 cut(s) 142, 214, 883
BspACI CCGC 1 cut(s) 541
BspANI GGCC 1 cut(s) 786
BspCNI CTCAG 3 cut(s) 22, 117, 151
BspFNI CGCG 1 cut(s) 418
BspLI GGNNCC 4 cut(s) 144, 216, 358, 885
BspMAI CTGCAG 1 cut(s) 900
BspPI GGATC 6 cut(s) 137, 150, 209, 222, 878, 891
BspT107I GGYRCC 1 cut(s) 356
BsrDI GCAATG 2 cut(s) 31, 201
BsrGI TGTACA 1 cut(s) 709
BsrI ACTGG 1 cut(s) 705
BssECI CCNNGG 2 cut(s) 210, 787
BssMI GATC 3 cut(s) 142, 214, 883
BssSI CACGAG 1 cut(s) 275
BssT1I CCWWGG 2 cut(s) 210, 787
Bst2BI CACGAG 1 cut(s) 275
Bst2UI CCWGG 1 cut(s) 339
Bst4CI ACNGT 1 cut(s) 841
BstAUI TGTACA 1 cut(s) 709
BstC8I GCNNGC 4 cut(s) 230, 387, 391, 576
BstDEI CTNAG 3 cut(s) 9, 104, 138
BstF5I GGATG 2 cut(s) 289, 666
BstFNI CGCG 1 cut(s) 418
BstHHI GCGC 1 cut(s) 751
BstKTI GATC 3 cut(s) 145, 217, 886
BstMBI GATC 3 cut(s) 142, 214, 883
BstMWI GCNNNNNNNGC 2 cut(s) 809, 825
BstNI CCWGG 1 cut(s) 339
BstNSI RCATGY 4 cut(s) 89, 232, 578, 658
BstSCI CCNGG 2 cut(s) 337, 532
BstSFI CTRYAG 1 cut(s) 896
BstUI CGCG 1 cut(s) 418
BstX2I RGATCY 3 cut(s) 142, 214, 883
BstYI RGATCY 3 cut(s) 142, 214, 883
BsuI GTATCC 1 cut(s) 644
BsuRI GGCC 1 cut(s) 786
BtsCI GGATG 2 cut(s) 289, 666
BtsI GCAGTG 1 cut(s) 905
BtsIMutI CAGTG 4 cut(s) 17, 712, 846, 905
Cac8I GCNNGC 4 cut(s) 230, 387, 391, 576
CfoI GCGC 1 cut(s) 751
Cfr13I GGNCC 1 cut(s) 157
Csp6I GTAC 4 cut(s) 357, 367, 675, 710
CviAII CATG 7 cut(s) 86, 229, 544, 563, 575, 579, 655
CviJI RGCY 7 cut(s) 5, 48, 137, 786, 812, 828, 890
CviKI_1 RGCY 7 cut(s) 5, 48, 137, 786, 812, 828, 890
CviQI GTAC 4 cut(s) 357, 367, 675, 710
DdeI CTNAG 3 cut(s) 9, 104, 138
DpnI GATC 3 cut(s) 144, 216, 885
DpnII GATC 3 cut(s) 142, 214, 883
DraI TTTAAA 1 cut(s) 270
DrdI GACNNNNNNGTC 1 cut(s) 851
DseDI GACNNNNNNGTC 1 cut(s) 851
EaeI YGGCCR 1 cut(s) 784
Eco130I CCWWGG 2 cut(s) 210, 787
Eco47I GGWCC 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 337
EcoT14I CCWWGG 2 cut(s) 210, 787
EcoT22I ATGCAT 3 cut(s) 230, 576, 580
ErhI CCWWGG 2 cut(s) 210, 787
FaeI CATG 7 cut(s) 89, 232, 547, 566, 578, 582, 658
FalI AAGNNNNNCTT 2 cut(s) 812, 844
FaqI GGGAC 1 cut(s) 112
FatI CATG 7 cut(s) 85, 228, 543, 562, 574, 578, 654
FauNDI CATATG 1 cut(s) 362
FblI GTMKAC 1 cut(s) 309
FokI GGATG 2 cut(s) 296, 673
FspBI CTAG 2 cut(s) 198, 678
GlaI GCGC 1 cut(s) 750
HaeIII GGCC 1 cut(s) 786
HapII CCGG 1 cut(s) 533
HhaI GCGC 1 cut(s) 751
Hin1II CATG 7 cut(s) 89, 232, 547, 566, 578, 582, 658
Hin6I GCGC 1 cut(s) 749
HinP1I GCGC 1 cut(s) 749
HincII GTYRAC 3 cut(s) 310, 459, 688
HindII GTYRAC 3 cut(s) 310, 459, 688
HinfI GANTC 4 cut(s) 68, 100, 537, 689
HpaI GTTAAC 1 cut(s) 459
HpaII CCGG 1 cut(s) 533
HphI GGTGA 2 cut(s) 522, 560
Hpy166II GTNNAC 7 cut(s) 83, 310, 459, 530, 688, 710, 844
Hpy188I TCNGA 1 cut(s) 94
Hpy188III TCNNGA 5 cut(s) 80, 140, 146, 770, 856
Hpy8I GTNNAC 7 cut(s) 83, 310, 459, 530, 688, 710, 844
Hpy99I CGWCG 3 cut(s) 468, 492, 857
HpyAV CCTTC 3 cut(s) 416, 599, 623
HpyCH4III ACNGT 1 cut(s) 841
HpyCH4IV ACGT 2 cut(s) 466, 763
HpyF10VI GCNNNNNNNGC 2 cut(s) 809, 825
HpyF3I CTNAG 3 cut(s) 9, 104, 138
HpySE526I ACGT 2 cut(s) 466, 763
Hsp92II CATG 7 cut(s) 89, 232, 547, 566, 578, 582, 658
HspAI GCGC 1 cut(s) 749
KpnI GGTACC 1 cut(s) 360
KspAI GTTAAC 1 cut(s) 459
Kzo9I GATC 3 cut(s) 142, 214, 883
LmnI GCTCC 1 cut(s) 895
LweI GCATC 2 cut(s) 561, 790
MaeI CTAG 2 cut(s) 198, 678
MaeII ACGT 2 cut(s) 466, 763
MaeIII GTNAC 1 cut(s) 119
MalI GATC 3 cut(s) 144, 216, 885
MboI GATC 3 cut(s) 142, 214, 883
MboII GAAGA 1 cut(s) 638
MflI RGATCY 3 cut(s) 142, 214, 883
MlsI TGGCCA 1 cut(s) 786
MluCI AATT 9 cut(s) 31, 41, 56, 254, 329, 426, 516, 608, 777
MluI ACGCGT 1 cut(s) 416
MluNI TGGCCA 1 cut(s) 786
MlyI GAGTC 3 cut(s) 94, 546, 683
MnlI CCTC 8 cut(s) 58, 100, 284, 447, 596, 878, 899, 903
Mox20I TGGCCA 1 cut(s) 786
Mph1103I ATGCAT 3 cut(s) 230, 576, 580
MroXI GAANNNNTTC 2 cut(s) 291, 496
MscI TGGCCA 1 cut(s) 786
MseI TTAA 8 cut(s) 129, 162, 269, 402, 432, 458, 479, 644
MslI CAYNNNNRTG 1 cut(s) 659
Msp20I TGGCCA 1 cut(s) 786
MspI CCGG 1 cut(s) 533
MspR9I CCNGG 2 cut(s) 339, 534
MssI GTTTAAAC 1 cut(s) 270
Mva1269I GAATGC 1 cut(s) 230
MvaI CCWGG 1 cut(s) 339
MvnI CGCG 1 cut(s) 418
MwoI GCNNNNNNNGC 2 cut(s) 809, 825
NciI CCSGG 1 cut(s) 534
NdeI CATATG 1 cut(s) 362
NdeII GATC 3 cut(s) 142, 214, 883
NlaIII CATG 7 cut(s) 89, 232, 547, 566, 578, 582, 658
NlaIV GGNNCC 4 cut(s) 144, 216, 358, 885
NmuCI GTSAC 1 cut(s) 119
NsiI ATGCAT 3 cut(s) 230, 576, 580
NspI RCATGY 4 cut(s) 89, 232, 578, 658
PaeI GCATGC 2 cut(s) 232, 578
PciI ACATGT 1 cut(s) 654
PctI GAATGC 1 cut(s) 230
PdmI GAANNNNTTC 2 cut(s) 291, 496
PfeI GAWTC 1 cut(s) 68
PleI GAGTC 3 cut(s) 94, 545, 683
PmeI GTTTAAAC 1 cut(s) 270
PpsI GAGTC 3 cut(s) 94, 545, 683
PscI ACATGT 1 cut(s) 654
PsiI TTATAA 1 cut(s) 261
Psp6I CCWGG 1 cut(s) 337
PspGI CCWGG 1 cut(s) 337
PspN4I GGNNCC 4 cut(s) 144, 216, 358, 885
PspPI GGNCC 1 cut(s) 157
PstI CTGCAG 1 cut(s) 900
PsuI RGATCY 3 cut(s) 142, 214, 883
RsaI GTAC 4 cut(s) 358, 368, 676, 711
RsaNI GTAC 4 cut(s) 357, 367, 675, 710
RseI CAYNNNNRTG 1 cut(s) 659
SalI GTCGAC 1 cut(s) 308
SaqAI TTAA 8 cut(s) 129, 162, 269, 402, 432, 458, 479, 644
Sau3AI GATC 3 cut(s) 142, 214, 883
Sau96I GGNCC 1 cut(s) 157
SchI GAGTC 3 cut(s) 94, 546, 683
ScrFI CCNGG 2 cut(s) 339, 534
SfaNI GCATC 2 cut(s) 561, 790
SfcI CTRYAG 1 cut(s) 896
SinI GGWCC 1 cut(s) 157
SmiMI CAYNNNNRTG 1 cut(s) 659
SpeI ACTAGT 1 cut(s) 677
SphI GCATGC 2 cut(s) 232, 578
Sse9I AATT 9 cut(s) 31, 41, 56, 254, 329, 426, 516, 608, 777
SsiI CCGC 1 cut(s) 541
SspI AATATT 1 cut(s) 152
SspMI CTAG 2 cut(s) 198, 678
StyD4I CCNGG 2 cut(s) 337, 532
StyI CCWWGG 2 cut(s) 210, 787
TaaI ACNGT 1 cut(s) 841
TaiI ACGT 2 cut(s) 469, 766
TaqI TCGA 2 cut(s) 309, 855
TasI AATT 9 cut(s) 31, 41, 56, 254, 329, 426, 516, 608, 777
TatI WGTACW 2 cut(s) 674, 709
TfiI GAWTC 1 cut(s) 68
Tru1I TTAA 8 cut(s) 129, 162, 269, 402, 432, 458, 479, 644
Tru9I TTAA 8 cut(s) 129, 162, 269, 402, 432, 458, 479, 644
TscAI CASTG 4 cut(s) 17, 712, 846, 905
TseFI GTSAC 1 cut(s) 119
Tsp45I GTSAC 1 cut(s) 119
TspDTI ATGAA 2 cut(s) 300, 322
TspRI CASTG 4 cut(s) 17, 712, 846, 905
VpaK11BI GGWCC 1 cut(s) 157
XapI RAATTY 5 cut(s) 31, 56, 254, 329, 777
XceI RCATGY 4 cut(s) 89, 232, 578, 658
XmiI GTMKAC 1 cut(s) 309
XmnI GAANNNNTTC 2 cut(s) 291, 496
XspI CTAG 2 cut(s) 198, 678
Zsp2I ATGCAT 3 cut(s) 230, 576, 580
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.