Rroxscaffold_2G00090090

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000002
OG0000415
Physical Location & Seq
Forward (+)
11926265 .. 11926849
585 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_2G00090090.1

Sequence Viewer

Length: 585 bp
ATGTTGGTCGACCATGAAGAGATGTGCAAACGTTGCGGAGAAAAACCCAGTACAGAAGATGGTTATAGACGACTTTTTACTGGTGTTATATTTTTCCCTAGACGGCGTGCTCGTGTTCTCACGAGCATTACAAAATGTTCAAAAATTGGGGAAGATATCCAAGTGGAGAAAAAAACCGGCTCATCCCCGCGTAACAAAATTACAATTTTAGTTCTGTTCGGAGTACCAGACCTGTTCATCGGTGGAACTTTGGCACACGAGATGATGCATGCATGGTTGCACCTTCAAAATTGTAGGAAAAAATTGGAAGAAAAGGTCGAAGAAGGGATTTGTGAAGTGATGGACCACAAGTGGATGGAAATGTTCTGTTCCAGTGATGATCTTGATCACTCCTCATACAAAACCTATAAGCAGGGCCAGTTTAAGAGAAAATTGAAACAATATCTGGAAGAATCAACGGAAACCCGTCAAGACATGTATGGCCAAGGATACAGAGAGGCTACTAGGGCAGTTGAAATGTTTGGCTTCCAAACTACTCTAAACCATATTGTTCAAAAAGGAAGTTTCCCTCACCGCGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

194

Amino Acids

22.72

Weight (kDa)

8.68

Isoelectric Point (pI)

48.08

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 38 - 184 1e-33 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 9
AccII CGCG 2 cut(s) 190, 576
AciI CCGC 3 cut(s) 36, 188, 574
AclI AACGTT 1 cut(s) 31
AcoI YGGCCR 1 cut(s) 481
AfaI GTAC 2 cut(s) 52, 225
AflIII ACRYGT 1 cut(s) 474
AgsI TTSAA 5 cut(s) 141, 287, 436, 515, 554
Alw21I GWGCWC 1 cut(s) 112
AoxI GGCC 2 cut(s) 415, 481
AspS9I GGNCC 2 cut(s) 343, 415
AsuHPI GGTGA 1 cut(s) 563
AvaII GGWCC 1 cut(s) 343
BalI TGGCCA 1 cut(s) 483
BauI CACGAG 3 cut(s) 111, 121, 257
Bbv12I GWGCWC 1 cut(s) 112
BccI CCATC 3 cut(s) 53, 334, 349
BceAI ACGGC 1 cut(s) 119
BciVI GTATCC 1 cut(s) 482
BclI TGATCA 1 cut(s) 385
BfaI CTAG 2 cut(s) 99, 504
BfuI GTATCC 1 cut(s) 482
Bme18I GGWCC 1 cut(s) 343
BmgT120I GGNCC 2 cut(s) 343, 415
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 255
BmuI ACTGGG 1 cut(s) 42
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 484
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 4 cut(s) 48, 85, 372, 418
Bse8I GATNNNNATC 1 cut(s) 384
BseDI CCNNGG 1 cut(s) 484
BseGI GGATG 2 cut(s) 182, 360
BseJI GATNNNNATC 1 cut(s) 384
BseNI ACTGG 4 cut(s) 48, 85, 372, 418
BseRI GAGGAG 1 cut(s) 382
Bsh1236I CGCG 2 cut(s) 190, 576
BshFI GGCC 2 cut(s) 417, 483
BsiHKAI GWGCWC 1 cut(s) 112
BsiSI CCGG 1 cut(s) 177
BsnI GGCC 2 cut(s) 417, 483
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 2 cut(s) 379, 385
BspACI CCGC 3 cut(s) 36, 188, 574
BspANI GGCC 2 cut(s) 417, 483
BspFNI CGCG 2 cut(s) 190, 576
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 4 cut(s) 48, 85, 372, 418
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 484
BssMI GATC 2 cut(s) 379, 385
BssSI CACGAG 3 cut(s) 111, 121, 257
BssT1I CCWWGG 1 cut(s) 484
Bst2BI CACGAG 3 cut(s) 111, 121, 257
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 33
BstC8I GCNNGC 2 cut(s) 108, 270
BstF5I GGATG 2 cut(s) 182, 360
BstFNI CGCG 2 cut(s) 190, 576
BstKTI GATC 2 cut(s) 382, 388
BstMBI GATC 2 cut(s) 379, 385
BstMWI GCNNNNNNNGC 2 cut(s) 33, 506
BstNSI RCATGY 2 cut(s) 272, 478
BstUI CGCG 2 cut(s) 190, 576
BsuI GTATCC 1 cut(s) 482
BsuRI GGCC 2 cut(s) 417, 483
BtsCI GGATG 2 cut(s) 182, 360
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 2 cut(s) 108, 270
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 2 cut(s) 343, 415
Csp6I GTAC 2 cut(s) 51, 224
CviAII CATG 4 cut(s) 14, 269, 273, 475
CviJI RGCY 5 cut(s) 180, 417, 483, 500, 525
CviKI_1 RGCY 5 cut(s) 180, 417, 483, 500, 525
CviQI GTAC 2 cut(s) 51, 224
DpnI GATC 2 cut(s) 381, 387
DpnII GATC 2 cut(s) 379, 385
EaeI YGGCCR 1 cut(s) 481
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 484
Eco32I GATATC 1 cut(s) 157
Eco47I GGWCC 1 cut(s) 343
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 2 cut(s) 270, 274
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 4 cut(s) 17, 272, 276, 478
FatI CATG 4 cut(s) 13, 268, 272, 474
FauI CCCGC 1 cut(s) 195
FbaI TGATCA 1 cut(s) 385
FblI GTMKAC 1 cut(s) 9
FokI GGATG 2 cut(s) 169, 367
FspBI CTAG 2 cut(s) 99, 504
HaeIII GGCC 2 cut(s) 417, 483
HapII CCGG 1 cut(s) 177
Hin1II CATG 4 cut(s) 17, 272, 276, 478
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 1 cut(s) 452
HpaII CCGG 1 cut(s) 177
HphI GGTGA 1 cut(s) 563
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 1 cut(s) 221
Hpy188III TCNNGA 4 cut(s) 121, 383, 446, 470
Hpy8I GTNNAC 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 293, 317
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 4 cut(s) 27, 268, 272, 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 506
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 4 cut(s) 17, 272, 276, 478
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 2 cut(s) 379, 385
LpnPI CCDG 9 cut(s) 61, 66, 190, 240, 245, 385, 398, 431, 431
LweI GCATC 1 cut(s) 255
MaeI CTAG 2 cut(s) 99, 504
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 1 cut(s) 191
MalI GATC 2 cut(s) 381, 387
MboI GATC 2 cut(s) 379, 385
MboII GAAGA 6 cut(s) 29, 68, 164, 320, 332, 461
MhlI GDGCHC 1 cut(s) 112
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 6 cut(s) 144, 198, 204, 289, 302, 431
MluNI TGGCCA 1 cut(s) 483
MnlI CCTC 3 cut(s) 403, 490, 579
Mox20I TGGCCA 1 cut(s) 483
Mph1103I ATGCAT 2 cut(s) 270, 274
MscI TGGCCA 1 cut(s) 483
MseI TTAA 1 cut(s) 423
Msp20I TGGCCA 1 cut(s) 483
MspI CCGG 1 cut(s) 177
MvnI CGCG 2 cut(s) 190, 576
MwoI GCNNNNNNNGC 2 cut(s) 33, 506
NdeII GATC 2 cut(s) 379, 385
NlaIII CATG 4 cut(s) 17, 272, 276, 478
NsiI ATGCAT 2 cut(s) 270, 274
NspI RCATGY 2 cut(s) 272, 478
PaeI GCATGC 1 cut(s) 272
PciI ACATGT 1 cut(s) 474
PcsI WCGNNNNNNNCGW 1 cut(s) 109
PfeI GAWTC 1 cut(s) 452
PscI ACATGT 1 cut(s) 474
Psp1406I AACGTT 1 cut(s) 31
PspPI GGNCC 2 cut(s) 343, 415
RsaI GTAC 2 cut(s) 52, 225
RsaNI GTAC 2 cut(s) 51, 224
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 423
Sau3AI GATC 2 cut(s) 379, 385
Sau96I GGNCC 2 cut(s) 343, 415
SduI GDGCHC 1 cut(s) 112
SetI ASST 5 cut(s) 34, 234, 285, 318, 407
SfaNI GCATC 1 cut(s) 255
SinI GGWCC 1 cut(s) 343
SphI GCATGC 1 cut(s) 272
Sse9I AATT 6 cut(s) 144, 198, 204, 289, 302, 431
SsiI CCGC 3 cut(s) 36, 188, 574
SspMI CTAG 2 cut(s) 99, 504
StyI CCWWGG 1 cut(s) 484
TaiI ACGT 1 cut(s) 34
TaqI TCGA 2 cut(s) 9, 318
TasI AATT 6 cut(s) 144, 198, 204, 289, 302, 431
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 379
TspDTI ATGAA 2 cut(s) 30, 226
TspGWI ACGGA 1 cut(s) 473
TspRI CASTG 1 cut(s) 379
VpaK11BI GGWCC 1 cut(s) 343
XceI RCATGY 2 cut(s) 272, 478
XmiI GTMKAC 1 cut(s) 9
XspI CTAG 2 cut(s) 99, 504
Zsp2I ATGCAT 2 cut(s) 270, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.