Rorug03G0077500

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000003
OG0000415
Physical Location & Seq
Reverse (-)
6072309 .. 6075320
3012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug03G0077500.1

Sequence Viewer

Length: 549 bp
ATGCATGTAATGGATCATATAGCTTCTAGAGAATGTGATCTGGAGTTTGACTTGGAAAGTGGTGAGACTGAATCCAGCAGTGAAGAAGATGGAAGTGAAGAGTCCGGTTCCCATGGTAAACATTTCAATAAATTATTGAATAGGGGAAGAAGTGGATACTTGTGTATTGAAGGATCAATAACAGCTAATGATGTTGTAGAAAGCAATAAGGTATCAAATATTGATGAGACTAGGAATGTGATAGAAGGAGGAATTGAGAAGAGAATGGTGGATAAACAGAAAATGAAAGGCTCTGAGAAGCATCCCAAGCCACCTAGGCCTCCTACTGGTCCATCATTACATGCAGCCGATATAGAGTTGGTTAAAGAAATTTCAAAGCATACTAGGTTGCGGCGTGCAAGGAGAGAAAGACTGAAAGCATTGAAGAATATGAAAACTGATAGAAGTCATAGAACATCATCAGCAAATATCAACTTTTTGGCAATAATCATCACAGCTATCTTCTTCTTTGTGATAATCTTCCAAGGAGTTTTGGGTGCTCGTGTATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

182

Amino Acids

20.41

Weight (kDa)

8.55

Isoelectric Point (pI)

66.55

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 391
AclWI GGATC 2 cut(s) 21, 181
AcsI RAATTY 1 cut(s) 369
AfiI CCNNNNNNNGG 1 cut(s) 326
AgsI TTSAA 5 cut(s) 127, 139, 170, 375, 424
AluBI AGCT 3 cut(s) 23, 185, 497
AluI AGCT 3 cut(s) 23, 185, 497
Alw21I GWGCWC 1 cut(s) 541
Alw26I GTCTC 2 cut(s) 59, 221
AlwI GGATC 2 cut(s) 21, 181
AoxI GGCC 1 cut(s) 317
ApeKI GCWGC 1 cut(s) 344
ApoI RAATTY 1 cut(s) 369
AspA2I CCTAGG 1 cut(s) 314
AspS9I GGNCC 1 cut(s) 329
AsuHPI GGTGA 1 cut(s) 74
AvaII GGWCC 1 cut(s) 329
AvrII CCTAGG 1 cut(s) 314
BaeI ACNNNNGTAYC 2 cut(s) 148, 181
BauI CACGAG 1 cut(s) 540
Bbv12I GWGCWC 1 cut(s) 541
BbvI GCAGC 1 cut(s) 356
BccI CCATC 2 cut(s) 83, 340
BciVI GTATCC 1 cut(s) 149
BcoDI GTCTC 2 cut(s) 59, 221
BfaI CTAG 4 cut(s) 27, 231, 315, 384
BfuI GTATCC 1 cut(s) 149
BglI GCCNNNNNGGC 1 cut(s) 316
BisI GCNGC 2 cut(s) 345, 392
BlnI CCTAGG 1 cut(s) 314
BlsI GCNGC 2 cut(s) 346, 393
Bme18I GGWCC 1 cut(s) 329
BmgT120I GGNCC 1 cut(s) 329
BmiI GGNNCC 1 cut(s) 109
BmsI GCATC 1 cut(s) 310
BpmI CTGGAG 1 cut(s) 62
BsaJI CCNNGG 3 cut(s) 112, 314, 523
BsaWI WCCGGW 1 cut(s) 104
Bsc4I CCNNNNNNNGG 1 cut(s) 326
Bse1I ACTGG 1 cut(s) 331
BseDI CCNNGG 3 cut(s) 112, 314, 523
BseGI GGATG 1 cut(s) 301
BseLI CCNNNNNNNGG 1 cut(s) 326
BseMII CTCAG 1 cut(s) 285
BseNI ACTGG 1 cut(s) 331
BseXI GCAGC 1 cut(s) 356
BshFI GGCC 1 cut(s) 319
BsiHKAI GWGCWC 1 cut(s) 541
BsiSI CCGG 1 cut(s) 105
BslI CCNNNNNNNGG 1 cut(s) 326
BsmAI GTCTC 2 cut(s) 59, 221
BsnI GGCC 1 cut(s) 319
Bsp1286I GDGCHC 1 cut(s) 541
Bsp143I GATC 3 cut(s) 13, 37, 173
Bsp19I CCATGG 1 cut(s) 112
BspACI CCGC 1 cut(s) 391
BspANI GGCC 1 cut(s) 319
BspCNI CTCAG 1 cut(s) 286
BspLI GGNNCC 1 cut(s) 109
BspPI GGATC 2 cut(s) 21, 181
BsrI ACTGG 1 cut(s) 331
BssECI CCNNGG 3 cut(s) 112, 314, 523
BssMI GATC 3 cut(s) 13, 37, 173
BssSI CACGAG 1 cut(s) 540
BssT1I CCWWGG 3 cut(s) 112, 314, 523
Bst2BI CACGAG 1 cut(s) 540
Bst6I CTCTTC 2 cut(s) 93, 254
BstC8I GCNNGC 1 cut(s) 396
BstDEI CTNAG 1 cut(s) 294
BstDSI CCRYGG 1 cut(s) 112
BstF5I GGATG 1 cut(s) 301
BstKTI GATC 3 cut(s) 16, 40, 176
BstMAI GTCTC 2 cut(s) 59, 221
BstMBI GATC 3 cut(s) 13, 37, 173
BstMWI GCNNNNNNNGC 2 cut(s) 307, 316
BstNSI RCATGY 2 cut(s) 8, 344
BstV1I GCAGC 1 cut(s) 356
BsuI GTATCC 1 cut(s) 149
BsuRI GGCC 1 cut(s) 319
BtgI CCRYGG 1 cut(s) 112
BtsCI GGATG 1 cut(s) 301
BtsI GCAGTG 1 cut(s) 85
BtsIMutI CAGTG 1 cut(s) 85
Cac8I GCNNGC 1 cut(s) 396
Cfr13I GGNCC 1 cut(s) 329
CviAII CATG 3 cut(s) 5, 113, 341
CviJI RGCY 7 cut(s) 23, 185, 291, 310, 319, 347, 497
CviKI_1 RGCY 7 cut(s) 23, 185, 291, 310, 319, 347, 497
DdeI CTNAG 1 cut(s) 294
DpnI GATC 3 cut(s) 15, 39, 175
DpnII GATC 3 cut(s) 13, 37, 173
Eam1104I CTCTTC 2 cut(s) 93, 254
EarI CTCTTC 2 cut(s) 93, 254
Eco130I CCWWGG 3 cut(s) 112, 314, 523
Eco147I AGGCCT 1 cut(s) 319
Eco47I GGWCC 1 cut(s) 329
EcoT14I CCWWGG 3 cut(s) 112, 314, 523
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 3 cut(s) 112, 314, 523
FaeI CATG 3 cut(s) 8, 116, 344
FatI CATG 3 cut(s) 4, 112, 340
Fnu4HI GCNGC 2 cut(s) 345, 392
FokI GGATG 1 cut(s) 288
Fsp4HI GCNGC 2 cut(s) 345, 392
FspBI CTAG 4 cut(s) 27, 231, 315, 384
GluI GCNGC 2 cut(s) 345, 392
GsuI CTGGAG 1 cut(s) 62
HaeIII GGCC 1 cut(s) 319
HapII CCGG 1 cut(s) 105
Hin1II CATG 3 cut(s) 8, 116, 344
HinfI GANTC 2 cut(s) 71, 101
HpaII CCGG 1 cut(s) 105
HphI GGTGA 1 cut(s) 74
Hpy166II GTNNAC 1 cut(s) 119
Hpy188I TCNGA 1 cut(s) 295
Hpy188III TCNNGA 2 cut(s) 27, 41
Hpy8I GTNNAC 1 cut(s) 119
HpyAV CCTTC 2 cut(s) 164, 239
HpyCH4V TGCA 3 cut(s) 4, 344, 398
HpyF10VI GCNNNNNNNGC 2 cut(s) 307, 316
HpyF3I CTNAG 1 cut(s) 294
Hsp92II CATG 3 cut(s) 8, 116, 344
Kzo9I GATC 3 cut(s) 13, 37, 173
LpnPI CCDG 4 cut(s) 26, 88, 118, 312
Lsp1109I GCAGC 1 cut(s) 356
LweI GCATC 1 cut(s) 310
MaeI CTAG 4 cut(s) 27, 231, 315, 384
MalI GATC 3 cut(s) 15, 39, 175
MboI GATC 3 cut(s) 13, 37, 173
MboII GAAGA 9 cut(s) 95, 98, 110, 159, 271, 436, 493, 496, 511
MhlI GDGCHC 1 cut(s) 541
MluCI AATT 3 cut(s) 131, 252, 369
MlyI GAGTC 1 cut(s) 110
MnlI CCTC 2 cut(s) 242, 330
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 1 cut(s) 363
MspI CCGG 1 cut(s) 105
MwoI GCNNNNNNNGC 2 cut(s) 307, 316
NcoI CCATGG 1 cut(s) 112
NdeII GATC 3 cut(s) 13, 37, 173
NlaIII CATG 3 cut(s) 8, 116, 344
NlaIV GGNNCC 1 cut(s) 109
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 2 cut(s) 8, 344
PceI AGGCCT 1 cut(s) 319
PfeI GAWTC 1 cut(s) 71
PkrI GCNGC 2 cut(s) 346, 393
PleI GAGTC 1 cut(s) 109
PpsI GAGTC 1 cut(s) 109
PspN4I GGNNCC 1 cut(s) 109
PspPI GGNCC 1 cut(s) 329
SaqAI TTAA 1 cut(s) 363
SatI GCNGC 2 cut(s) 345, 392
Sau3AI GATC 3 cut(s) 13, 37, 173
Sau96I GGNCC 1 cut(s) 329
SchI GAGTC 1 cut(s) 110
SduI GDGCHC 1 cut(s) 541
SetI ASST 6 cut(s) 25, 187, 213, 316, 389, 499
SfaNI GCATC 1 cut(s) 310
SinI GGWCC 1 cut(s) 329
Sse9I AATT 3 cut(s) 131, 252, 369
SseBI AGGCCT 1 cut(s) 319
SsiI CCGC 1 cut(s) 391
SspI AATATT 1 cut(s) 220
SspMI CTAG 4 cut(s) 27, 231, 315, 384
StuI AGGCCT 1 cut(s) 319
StyI CCWWGG 3 cut(s) 112, 314, 523
TasI AATT 3 cut(s) 131, 252, 369
TauI GCSGC 1 cut(s) 394
TfiI GAWTC 1 cut(s) 71
Tru1I TTAA 1 cut(s) 363
Tru9I TTAA 1 cut(s) 363
TscAI CASTG 1 cut(s) 85
TseI GCWGC 1 cut(s) 344
TspDTI ATGAA 2 cut(s) 299, 446
TspRI CASTG 1 cut(s) 85
VpaK11BI GGWCC 1 cut(s) 329
XapI RAATTY 1 cut(s) 369
XbaI TCTAGA 1 cut(s) 26
XceI RCATGY 2 cut(s) 8, 344
XmaJI CCTAGG 1 cut(s) 314
XspI CTAG 4 cut(s) 27, 231, 315, 384
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.