Rw4G011910

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr4
OG0000415
Physical Location & Seq
Forward (+)
28142467 .. 28144645
2179 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw4G011910.1

Sequence Viewer

Length: 813 bp
ATGATCCATGGTGTGATACTTGTTCAAAATACGCGGTTTAGAGAAGAATATCTGAGATCTGTAATAAATATTGAAAAAGCCGTGAAGTTGCTGCTAGGAGACAGAATCATAGTTTTGTTTGGGAAACCAGATGTAGAAATGGGAGGATTGTTGGCACATGAGATGATGCATGGATGGTTGAATCTTCAAGGTAAACCATGGGAATGGGAACGAAGCGTTGAAGAAGGTATTTGCCAAGTAATGTATTACAAATGGTTGCAATGGTTTTCTTCTAGAGGTTATAACTCTTCGCACAAAACCAATGAGCAAGTTCAACATACAAGAATGTTGAAAGAGTACTTGGCAGAGAAGATTGAGTGTTGGAATGATGAAGTTTATGGTCAAGGATTCATAAATGCTATGCGTGCTATTGAAATAGTTGGATTCAAAACGACGGTGGATCATATCGTCAAAGATGGAACTCTCCCTGTGGTTCCACCGGTTGCCCCGGTTGTGATGGGGAAGAAGAAGAAGACCACCACCACCACCACTGAGTTAACGAGGAAGAAGACCGCCTGTGCTCCAATAACTGAGCTGTTATGGAAGGCGACTAGCAGCACAACTAAATCATTATGGAAGGCCACAGCTGCAACAATTACCAACTTATTATGGAAGGTCACAGCCGCCTGTGAAACACCGCGATTTGTTACTATGGAAGGACACCGCCACCACCAGTATCATCAGGGACAAAGCCATAACAACTTACATGTTCCCATGGCCGCATCTCCTACTCTCCTTCTTGTATTTATTGGGATATTAATCGAACACAAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

270

Amino Acids

30.86

Weight (kDa)

9.17

Isoelectric Point (pI)

49.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 35 - 150 1.1e-27 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 282
AccII CGCG 2 cut(s) 34, 679
AciI CCGC 6 cut(s) 34, 552, 663, 677, 703, 759
AclWI GGATC 1 cut(s) 447
AcoI YGGCCR 1 cut(s) 756
AfaI GTAC 1 cut(s) 338
AflIII ACRYGT 1 cut(s) 745
AgeI ACCGGT 1 cut(s) 478
AgsI TTSAA 9 cut(s) 26, 74, 181, 188, 221, 314, 331, 413, 427
AjuI GAANNNNNNNTTGG 2 cut(s) 323, 355
AluBI AGCT 2 cut(s) 574, 626
AluI AGCT 2 cut(s) 574, 626
Alw21I GWGCWC 1 cut(s) 562
Alw26I GTCTC 1 cut(s) 93
AlwI GGATC 1 cut(s) 447
AoxI GGCC 2 cut(s) 618, 756
ApeKI GCWGC 3 cut(s) 91, 594, 626
AseI ATTAAT 1 cut(s) 797
AsiGI ACCGGT 1 cut(s) 478
AsuC2I CCSGG 1 cut(s) 488
BbsI GAAGAC 2 cut(s) 518, 554
Bbv12I GWGCWC 1 cut(s) 562
BbvI GCAGC 3 cut(s) 78, 606, 613
BccI CCATC 3 cut(s) 168, 449, 490
BceAI ACGGC 1 cut(s) 65
BcnI CCSGG 1 cut(s) 488
BcoDI GTCTC 1 cut(s) 93
BfaI CTAG 3 cut(s) 95, 273, 591
BglII AGATCT 1 cut(s) 56
BisI GCNGC 5 cut(s) 92, 595, 627, 663, 759
BlsI GCNGC 5 cut(s) 93, 596, 628, 664, 760
BmcAI AGTACT 1 cut(s) 338
Bme1390I CCNGG 1 cut(s) 488
BmiI GGNNCC 1 cut(s) 474
BmrFI CCNGG 1 cut(s) 488
BmsI GCATC 2 cut(s) 156, 770
BpiI GAAGAC 2 cut(s) 518, 554
BpuMI CCSGG 1 cut(s) 488
BsaBI GATNNNNATC 1 cut(s) 797
BsaJI CCNNGG 4 cut(s) 7, 197, 486, 753
BsaWI WCCGGW 1 cut(s) 478
Bse118I RCCGGY 1 cut(s) 478
Bse1I ACTGG 1 cut(s) 712
Bse3DI GCAATG 1 cut(s) 266
Bse8I GATNNNNATC 1 cut(s) 797
BseDI CCNNGG 4 cut(s) 7, 197, 486, 753
BseGI GGATG 1 cut(s) 179
BseJI GATNNNNATC 1 cut(s) 797
BseMI GCAATG 1 cut(s) 266
BseMII CTCAG 3 cut(s) 44, 522, 561
BseNI ACTGG 1 cut(s) 712
BseXI GCAGC 3 cut(s) 78, 606, 613
Bsh1236I CGCG 2 cut(s) 34, 679
BshFI GGCC 2 cut(s) 620, 758
BshTI ACCGGT 1 cut(s) 478
BsiHKAI GWGCWC 1 cut(s) 562
BsiSI CCGG 2 cut(s) 479, 488
BslFI GGGAC 1 cut(s) 738
BsmAI GTCTC 1 cut(s) 93
BsmFI GGGAC 1 cut(s) 738
BsnI GGCC 2 cut(s) 620, 758
Bsp1286I GDGCHC 1 cut(s) 562
Bsp143I GATC 3 cut(s) 3, 56, 439
Bsp19I CCATGG 3 cut(s) 7, 197, 753
BspACI CCGC 6 cut(s) 34, 552, 663, 677, 703, 759
BspANI GGCC 2 cut(s) 620, 758
BspCNI CTCAG 3 cut(s) 45, 523, 562
BspFNI CGCG 2 cut(s) 34, 679
BspLI GGNNCC 1 cut(s) 474
BspPI GGATC 1 cut(s) 447
BsrDI GCAATG 1 cut(s) 266
BsrFI RCCGGY 1 cut(s) 478
BsrI ACTGG 1 cut(s) 712
BssAI RCCGGY 1 cut(s) 478
BssECI CCNNGG 4 cut(s) 7, 197, 486, 753
BssMI GATC 3 cut(s) 3, 56, 439
BssT1I CCWWGG 3 cut(s) 7, 197, 753
Bst4CI ACNGT 1 cut(s) 436
Bst6I CTCTTC 1 cut(s) 292
BstC8I GCNNGC 1 cut(s) 405
BstDEI CTNAG 3 cut(s) 53, 531, 570
BstDSI CCRYGG 3 cut(s) 7, 197, 753
BstF5I GGATG 1 cut(s) 179
BstFNI CGCG 2 cut(s) 34, 679
BstKTI GATC 3 cut(s) 6, 59, 442
BstMAI GTCTC 1 cut(s) 93
BstMBI GATC 3 cut(s) 3, 56, 439
BstMWI GCNNNNNNNGC 2 cut(s) 404, 626
BstNSI RCATGY 1 cut(s) 749
BstSCI CCNGG 1 cut(s) 486
BstUI CGCG 2 cut(s) 34, 679
BstV1I GCAGC 3 cut(s) 78, 606, 613
BstV2I GAAGAC 2 cut(s) 518, 554
BstX2I RGATCY 1 cut(s) 56
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 1 cut(s) 56
BsuRI GGCC 2 cut(s) 620, 758
BtgI CCRYGG 3 cut(s) 7, 197, 753
BtsCI GGATG 1 cut(s) 179
BtsIMutI CAGTG 1 cut(s) 528
Cac8I GCNNGC 1 cut(s) 405
Cfr10I RCCGGY 1 cut(s) 478
Csp6I GTAC 1 cut(s) 337
CspAI ACCGGT 1 cut(s) 478
CviAII CATG 6 cut(s) 8, 158, 170, 198, 746, 754
CviJI RGCY 7 cut(s) 80, 574, 620, 626, 662, 732, 758
CviKI_1 RGCY 7 cut(s) 80, 574, 620, 626, 662, 732, 758
CviQI GTAC 1 cut(s) 337
DdeI CTNAG 3 cut(s) 53, 531, 570
DpnI GATC 3 cut(s) 5, 58, 441
DpnII GATC 3 cut(s) 3, 56, 439
EaeI YGGCCR 1 cut(s) 756
Eam1104I CTCTTC 1 cut(s) 292
EarI CTCTTC 1 cut(s) 292
Eco130I CCWWGG 3 cut(s) 7, 197, 753
EcoT14I CCWWGG 3 cut(s) 7, 197, 753
EcoT22I ATGCAT 1 cut(s) 171
ErhI CCWWGG 3 cut(s) 7, 197, 753
FaeI CATG 6 cut(s) 11, 161, 173, 201, 749, 757
FaqI GGGAC 1 cut(s) 738
FatI CATG 6 cut(s) 7, 157, 169, 197, 745, 753
Fnu4HI GCNGC 5 cut(s) 92, 595, 627, 663, 759
FokI GGATG 1 cut(s) 186
Fsp4HI GCNGC 5 cut(s) 92, 595, 627, 663, 759
FspBI CTAG 3 cut(s) 95, 273, 591
GluI GCNGC 5 cut(s) 92, 595, 627, 663, 759
HaeIII GGCC 2 cut(s) 620, 758
HapII CCGG 2 cut(s) 479, 488
Hin1II CATG 6 cut(s) 11, 161, 173, 201, 749, 757
HincII GTYRAC 1 cut(s) 537
HindII GTYRAC 1 cut(s) 537
HinfI GANTC 4 cut(s) 105, 181, 387, 423
HpaI GTTAAC 1 cut(s) 537
HpaII CCGG 2 cut(s) 479, 488
Hpy166II GTNNAC 2 cut(s) 194, 537
Hpy188I TCNGA 1 cut(s) 54
Hpy188III TCNNGA 1 cut(s) 273
Hpy8I GTNNAC 2 cut(s) 194, 537
Hpy99I CGWCG 1 cut(s) 436
HpyAV CCTTC 6 cut(s) 218, 577, 610, 646, 689, 785
HpyCH4III ACNGT 1 cut(s) 436
HpyCH4V TGCA 3 cut(s) 169, 259, 629
HpyF10VI GCNNNNNNNGC 2 cut(s) 404, 626
HpyF3I CTNAG 3 cut(s) 53, 531, 570
Hsp92II CATG 6 cut(s) 11, 161, 173, 201, 749, 757
KspAI GTTAAC 1 cut(s) 537
Kzo9I GATC 3 cut(s) 3, 56, 439
LmnI GCTCC 1 cut(s) 565
LpnPI CCDG 8 cut(s) 141, 480, 492, 501, 568, 679, 707, 725
Lsp1109I GCAGC 3 cut(s) 78, 606, 613
LweI GCATC 2 cut(s) 156, 770
MaeI CTAG 3 cut(s) 95, 273, 591
MaeIII GTNAC 2 cut(s) 655, 685
MalI GATC 3 cut(s) 5, 58, 441
MboI GATC 3 cut(s) 3, 56, 439
MflI RGATCY 1 cut(s) 56
MhlI GDGCHC 1 cut(s) 562
MluCI AATT 1 cut(s) 633
MmeI TCCRAC 2 cut(s) 341, 400
MnlI CCTC 3 cut(s) 137, 269, 534
Mph1103I ATGCAT 1 cut(s) 171
MseI TTAA 2 cut(s) 536, 797
MslI CAYNNNNRTG 1 cut(s) 202
MspA1I CMGCKG 1 cut(s) 626
MspI CCGG 2 cut(s) 479, 488
MspR9I CCNGG 1 cut(s) 488
MvnI CGCG 2 cut(s) 34, 679
MwoI GCNNNNNNNGC 2 cut(s) 404, 626
NciI CCSGG 1 cut(s) 488
NcoI CCATGG 3 cut(s) 7, 197, 753
NdeII GATC 3 cut(s) 3, 56, 439
NlaIII CATG 6 cut(s) 11, 161, 173, 201, 749, 757
NlaIV GGNNCC 1 cut(s) 474
NmuCI GTSAC 1 cut(s) 655
NsiI ATGCAT 1 cut(s) 171
NspI RCATGY 1 cut(s) 749
PciI ACATGT 1 cut(s) 745
PfeI GAWTC 4 cut(s) 105, 181, 387, 423
PinAI ACCGGT 1 cut(s) 478
PkrI GCNGC 5 cut(s) 93, 596, 628, 664, 760
PscI ACATGT 1 cut(s) 745
PshBI ATTAAT 1 cut(s) 797
PsiI TTATAA 1 cut(s) 282
PspN4I GGNNCC 1 cut(s) 474
PsuI RGATCY 1 cut(s) 56
PvuII CAGCTG 1 cut(s) 626
RsaI GTAC 1 cut(s) 338
RsaNI GTAC 1 cut(s) 337
RseI CAYNNNNRTG 1 cut(s) 202
SaqAI TTAA 2 cut(s) 536, 797
SatI GCNGC 5 cut(s) 92, 595, 627, 663, 759
Sau3AI GATC 3 cut(s) 3, 56, 439
ScaI AGTACT 1 cut(s) 338
ScrFI CCNGG 1 cut(s) 488
SduI GDGCHC 1 cut(s) 562
SetI ASST 6 cut(s) 193, 229, 280, 576, 628, 657
SfaNI GCATC 2 cut(s) 156, 770
SmiMI CAYNNNNRTG 1 cut(s) 202
Sse9I AATT 1 cut(s) 633
SsiI CCGC 6 cut(s) 34, 552, 663, 677, 703, 759
SspI AATATT 1 cut(s) 70
SspMI CTAG 3 cut(s) 95, 273, 591
StyD4I CCNGG 1 cut(s) 486
StyI CCWWGG 3 cut(s) 7, 197, 753
TaaI ACNGT 1 cut(s) 436
TaqI TCGA 1 cut(s) 801
TasI AATT 1 cut(s) 633
TatI WGTACW 1 cut(s) 336
TauI GCSGC 2 cut(s) 665, 761
TfiI GAWTC 4 cut(s) 105, 181, 387, 423
Tru1I TTAA 2 cut(s) 536, 797
Tru9I TTAA 2 cut(s) 536, 797
TscAI CASTG 1 cut(s) 535
TseFI GTSAC 1 cut(s) 655
TseI GCWGC 3 cut(s) 91, 594, 626
Tsp45I GTSAC 1 cut(s) 655
TspDTI ATGAA 2 cut(s) 379, 384
TspRI CASTG 1 cut(s) 535
VspI ATTAAT 1 cut(s) 797
XbaI TCTAGA 1 cut(s) 272
XceI RCATGY 1 cut(s) 749
XspI CTAG 3 cut(s) 95, 273, 591
ZrmI AGTACT 1 cut(s) 338
Zsp2I ATGCAT 1 cut(s) 171
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.