MD14G1198500.v1.1

DA1-related 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr14
OG0000415
Physical Location & Seq
Forward (+)
28859417 .. 28861565
2149 bp
Loading structure...
UTR
Exon/CDS
Intron
MD14G1198500.v1.1.491

Sequence Viewer

Length: 765 bp
ATGATTCCATTTTGGAATTTTAATTATTGCAAGACACATGAATCTGATGGAACTCCAAGGTGTAATACGTGTGACAGATTCAAGACAACAGGGCAGAATGGATATGTGAATCTTGGAAATAACCAGCAGCTTTGCTCGGAATGTTTTTCCACTGCTATCTTGCATCCAAGTAAATGCAAACGTCTTATTGAAAATGTGCGCAAATTTTATAAAAAGTTAGGCCTCCAAGTGGATAAAAAAATTCCTATTTTATTGATCGACGACGATGAAATCAGAAGAATTCACCCAAATGAACAGATGCTGAAGGTGGTTGGTCTTACCACATACCCTCCTTACACTGTCATGACGATTTCAAAATGTTCAAGGAAAGGTGATAATGTTGAAGTGCAGAAAAAAGAAATCAAGTTGGCATCGGGCAAAGTGTCATCAATTTTACTTTTGTTCGGCCAACCAGAGGTGATGATCGGTGCAACTTTGGCGCATGAGATGATGCATGCATGGTTAGCTCTTCAAGAAGGCATTTGTGAAGTAATGGCACACATATGGTTGACATCGTGCTGTAAGAAGGGTTTGGAGTCATACAACCCCAGGCACGGGCATGCTGTATTTTTGGAAAACTTGCAAAAGTATGCAACATACAAAATAGAATCCAATGTGGACAAAAATTATGGACAAGGATTTAGAGAGGCTAACATGACGGTAACTAAATTTGGACTCCGAGAAACCATAGATTATATCATTCGAAATAAAACTCTCCCTCGATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0032182 GO:0043130
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like LIM

Protein Analysis

255

Amino Acids

29.13

Weight (kDa)

9.08

Isoelectric Point (pI)

36.98

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 73 - 247 3.9e-29 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 210
Acc16I TGCGCA 1 cut(s) 200
AcoI YGGCCR 1 cut(s) 445
AcsI RAATTY 5 cut(s) 16, 203, 240, 279, 707
AcuI CTGAAG 1 cut(s) 323
AfiI CCNNNNNNNGG 4 cut(s) 229, 454, 593, 594
AflIII ACRYGT 1 cut(s) 68
AgsI TTSAA 6 cut(s) 82, 191, 354, 363, 383, 512
AjnI CCWGG 1 cut(s) 587
AluBI AGCT 2 cut(s) 130, 506
AluI AGCT 2 cut(s) 130, 506
AlwNI CAGNNNCTG 1 cut(s) 301
AoxI GGCC 2 cut(s) 220, 445
ApeKI GCWGC 1 cut(s) 127
ApoI RAATTY 5 cut(s) 16, 203, 240, 279, 707
AspLEI GCGC 2 cut(s) 201, 481
AsuHPI GGTGA 3 cut(s) 275, 383, 469
AsuII TTCGAA 1 cut(s) 742
BbvI GCAGC 1 cut(s) 139
BccI CCATC 1 cut(s) 41
BciT130I CCWGG 1 cut(s) 589
BisI GCNGC 1 cut(s) 128
BlsI GCNGC 1 cut(s) 129
Bme1390I CCNGG 1 cut(s) 589
BmrFI CCNGG 1 cut(s) 589
BmsI GCATC 4 cut(s) 172, 288, 419, 480
Bpu14I TTCGAA 1 cut(s) 742
BsaAI YACGTR 1 cut(s) 69
BsaJI CCNNGG 2 cut(s) 56, 587
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 4 cut(s) 229, 454, 593, 594
BseBI CCWGG 1 cut(s) 589
BseDI CCNNGG 2 cut(s) 56, 587
BseGI GGATG 1 cut(s) 163
BseLI CCNNNNNNNGG 4 cut(s) 229, 454, 593, 594
BseXI GCAGC 1 cut(s) 139
BsgI GTGCAG 1 cut(s) 407
BshFI GGCC 2 cut(s) 222, 447
BslI CCNNNNNNNGG 4 cut(s) 229, 454, 593, 594
BsnI GGCC 2 cut(s) 222, 447
Bsp119I TTCGAA 1 cut(s) 742
Bsp143I GATC 2 cut(s) 255, 462
BspANI GGCC 2 cut(s) 222, 447
BspHI TCATGA 1 cut(s) 342
BspQI GCTCTTC 1 cut(s) 513
BspT104I TTCGAA 1 cut(s) 742
BssECI CCNNGG 2 cut(s) 56, 587
BssMI GATC 2 cut(s) 255, 462
BssT1I CCWWGG 1 cut(s) 56
Bst2UI CCWGG 1 cut(s) 589
Bst4CI ACNGT 2 cut(s) 340, 700
Bst6I CTCTTC 1 cut(s) 513
BstBAI YACGTR 1 cut(s) 69
BstBI TTCGAA 1 cut(s) 742
BstC8I GCNNGC 2 cut(s) 495, 600
BstF5I GGATG 1 cut(s) 163
BstHHI GCGC 2 cut(s) 201, 481
BstKTI GATC 2 cut(s) 258, 465
BstMBI GATC 2 cut(s) 255, 462
BstMWI GCNNNNNNNGC 2 cut(s) 476, 503
BstNI CCWGG 1 cut(s) 589
BstNSI RCATGY 2 cut(s) 497, 602
BstSCI CCNGG 1 cut(s) 587
BstV1I GCAGC 1 cut(s) 139
BsuRI GGCC 2 cut(s) 222, 447
BtsCI GGATG 1 cut(s) 163
BtsI GCAGTG 1 cut(s) 150
BtsIMutI CAGTG 2 cut(s) 150, 336
Cac8I GCNNGC 2 cut(s) 495, 600
CaiI CAGNNNCTG 1 cut(s) 301
CciI TCATGA 1 cut(s) 342
CfoI GCGC 2 cut(s) 201, 481
CviAII CATG 7 cut(s) 38, 343, 482, 494, 498, 599, 694
CviJI RGCY 5 cut(s) 130, 222, 447, 506, 689
CviKI_1 RGCY 5 cut(s) 130, 222, 447, 506, 689
DpnI GATC 2 cut(s) 257, 464
DpnII GATC 2 cut(s) 255, 462
EaeI YGGCCR 1 cut(s) 445
Eam1104I CTCTTC 1 cut(s) 513
EarI CTCTTC 1 cut(s) 513
Eco130I CCWWGG 1 cut(s) 56
Eco147I AGGCCT 1 cut(s) 222
Eco57I CTGAAG 1 cut(s) 323
EcoRI GAATTC 1 cut(s) 279
EcoRII CCWGG 1 cut(s) 587
EcoT14I CCWWGG 1 cut(s) 56
EcoT22I ATGCAT 2 cut(s) 495, 499
ErhI CCWWGG 1 cut(s) 56
FaeI CATG 7 cut(s) 41, 346, 485, 497, 501, 602, 697
FatI CATG 7 cut(s) 37, 342, 481, 493, 497, 598, 693
FauNDI CATATG 1 cut(s) 542
Fnu4HI GCNGC 1 cut(s) 128
FokI GGATG 1 cut(s) 150
Fsp4HI GCNGC 1 cut(s) 128
FspI TGCGCA 1 cut(s) 200
GlaI GCGC 2 cut(s) 200, 480
GluI GCNGC 1 cut(s) 128
HaeIII GGCC 2 cut(s) 222, 447
HhaI GCGC 2 cut(s) 201, 481
Hin1II CATG 7 cut(s) 41, 346, 485, 497, 501, 602, 697
Hin6I GCGC 2 cut(s) 199, 479
HinP1I GCGC 2 cut(s) 199, 479
HincII GTYRAC 1 cut(s) 549
HindII GTYRAC 1 cut(s) 549
HinfI GANTC 7 cut(s) 4, 41, 78, 109, 575, 647, 714
HphI GGTGA 3 cut(s) 275, 383, 469
Hpy166II GTNNAC 2 cut(s) 549, 658
Hpy188I TCNGA 4 cut(s) 46, 139, 275, 719
Hpy188III TCNNGA 3 cut(s) 82, 343, 512
Hpy8I GTNNAC 2 cut(s) 549, 658
Hpy99I CGWCG 2 cut(s) 263, 266
HpyAV CCTTC 3 cut(s) 298, 509, 559
HpyCH4III ACNGT 2 cut(s) 340, 700
HpyCH4IV ACGT 2 cut(s) 68, 181
HpyCH4V TGCA 9 cut(s) 30, 163, 177, 388, 470, 493, 497, 622, 632
HpyF10VI GCNNNNNNNGC 2 cut(s) 476, 503
HpySE526I ACGT 2 cut(s) 68, 181
Hsp92II CATG 7 cut(s) 41, 346, 485, 497, 501, 602, 697
HspAI GCGC 2 cut(s) 199, 479
Kzo9I GATC 2 cut(s) 255, 462
LguI GCTCTTC 1 cut(s) 513
LpnPI CCDG 5 cut(s) 75, 137, 465, 574, 601
Lsp1109I GCAGC 1 cut(s) 139
LweI GCATC 4 cut(s) 172, 288, 419, 480
MaeII ACGT 2 cut(s) 68, 181
MaeIII GTNAC 2 cut(s) 71, 700
MalI GATC 2 cut(s) 257, 464
MboI GATC 2 cut(s) 255, 462
MboII GAAGA 2 cut(s) 288, 500
MluCI AATT 8 cut(s) 16, 22, 203, 240, 279, 429, 664, 707
MlyI GAGTC 2 cut(s) 584, 708
MnlI CCTC 4 cut(s) 233, 339, 448, 679
Mph1103I ATGCAT 2 cut(s) 495, 499
MseI TTAA 1 cut(s) 21
MslI CAYNNNNRTG 4 cut(s) 288, 341, 541, 597
MspR9I CCNGG 1 cut(s) 589
MvaI CCWGG 1 cut(s) 589
MwoI GCNNNNNNNGC 2 cut(s) 476, 503
NdeI CATATG 1 cut(s) 542
NdeII GATC 2 cut(s) 255, 462
NlaIII CATG 7 cut(s) 41, 346, 485, 497, 501, 602, 697
NmuCI GTSAC 1 cut(s) 71
NsbI TGCGCA 1 cut(s) 200
NsiI ATGCAT 2 cut(s) 495, 499
NspI RCATGY 2 cut(s) 497, 602
NspV TTCGAA 1 cut(s) 742
PaeI GCATGC 2 cut(s) 497, 602
PagI TCATGA 1 cut(s) 342
PceI AGGCCT 1 cut(s) 222
PciSI GCTCTTC 1 cut(s) 513
PfeI GAWTC 5 cut(s) 4, 41, 78, 109, 647
PkrI GCNGC 1 cut(s) 129
PleI GAGTC 2 cut(s) 583, 708
PpsI GAGTC 2 cut(s) 583, 708
Ppu21I YACGTR 1 cut(s) 69
PsiI TTATAA 1 cut(s) 210
Psp6I CCWGG 1 cut(s) 587
PspGI CCWGG 1 cut(s) 587
PstNI CAGNNNCTG 1 cut(s) 301
RseI CAYNNNNRTG 4 cut(s) 288, 341, 541, 597
SapI GCTCTTC 1 cut(s) 513
SaqAI TTAA 1 cut(s) 21
SatI GCNGC 1 cut(s) 128
Sau3AI GATC 2 cut(s) 255, 462
SchI GAGTC 2 cut(s) 584, 708
ScrFI CCNGG 1 cut(s) 589
SetI ASST 8 cut(s) 62, 71, 132, 184, 309, 373, 459, 508
SfaNI GCATC 4 cut(s) 172, 288, 419, 480
SfuI TTCGAA 1 cut(s) 742
SmiMI CAYNNNNRTG 4 cut(s) 288, 341, 541, 597
SphI GCATGC 2 cut(s) 497, 602
Sse9I AATT 8 cut(s) 16, 22, 203, 240, 279, 429, 664, 707
SseBI AGGCCT 1 cut(s) 222
StuI AGGCCT 1 cut(s) 222
StyD4I CCNGG 1 cut(s) 587
StyI CCWWGG 1 cut(s) 56
TaaI ACNGT 2 cut(s) 340, 700
TaiI ACGT 2 cut(s) 71, 184
TaqI TCGA 3 cut(s) 258, 742, 760
TasI AATT 8 cut(s) 16, 22, 203, 240, 279, 429, 664, 707
TfiI GAWTC 5 cut(s) 4, 41, 78, 109, 647
Tru1I TTAA 1 cut(s) 21
Tru9I TTAA 1 cut(s) 21
TscAI CASTG 2 cut(s) 157, 343
TseFI GTSAC 1 cut(s) 71
TseI GCWGC 1 cut(s) 127
Tsp45I GTSAC 1 cut(s) 71
TspDTI ATGAA 3 cut(s) 54, 282, 306
TspRI CASTG 2 cut(s) 157, 343
XapI RAATTY 5 cut(s) 16, 203, 240, 279, 707
XceI RCATGY 2 cut(s) 497, 602
Zsp2I ATGCAT 2 cut(s) 495, 499
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.