FvH4_6g04440

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb6
OG0000415
Physical Location & Seq
Reverse (-)
2426528 .. 2428505
1978 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_6g04440.t1

Sequence Viewer

Length: 915 bp
ATGAGTGGAATTCTTCAAACGATACAAAAATATCCGAAAAGTGGAGGAGATATCAAAAAGGTTTGGAAGCTCGCACTAGGACGCACAATCATACTTTTGTTCGGCTTACCAGATATAAAATTAGGAGAGATCTTGGCTCATGAGATGATGCATGCATGGATACGTGGACAAGGTATACCGTATTTTGGAGGCTGTAAGAGAACGACTGAAGAAGGTTTATGCGACGTAATGGCACACAAATGGTTGGAGTGGTTTCCTTCCAGTGGTTTCGATAACTCGCACAAAACCATTCAACAAGTTCAATATACAGAAGAGCTGAGAGAGTCTTCAGTAGTAGGCATTCAGTGTAACATTGACGAGGTTTATGGCCGAGGATTCAAAGATGCTACACGAGCTATTGAAACATTTGGCTTTAAACCCACCCTAGATTATTTCATTAAATATAGAACTCTACCACAACCAATGATGGATAGTACATGCTCCGCCGCCTCATCCACCACCGCAAAAGAGCTGGAAGCGTTTATATTTGATGGAATTCGGTCTAGAGGATACAAGTTTTGTGTCCGAGTATTCATAGCTGGTATACGAGCTATTGGAACAGTTGGTTTTAGACCTACCTTAGATCATCTCATAACATATAGAAGTCTACCACAACTTATGATGGATAGCACATGCTCCACCAACGCATCCGCCACCTCCGCCGCTGCCGAGTTAACAAGAAAGAATACCAGTGCCACCCCGCAACCTGATCATGTATTATGCAAGGCGTCCGGTAATACAACTAAATCATATTGGGTTGCCACAACAATTGACTTCATAGGGAAGGCGGCCACCATCTGCGAAGCTATGGGGATTATTATGATGAAAGGATACCTCCTATCTATCGGTATTGGTATACAAATTGAACACAGTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

305

Amino Acids

33.65

Weight (kDa)

8.7

Isoelectric Point (pI)

30.91

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 29 - 145 1.8e-25 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 4 cut(s) 175, 583, 646, 895
AciI CCGC 8 cut(s) 483, 486, 501, 690, 699, 702, 740, 827
AcoI YGGCCR 2 cut(s) 367, 828
AcsI RAATTY 2 cut(s) 9, 534
AcuI CTGAAG 2 cut(s) 228, 312
AcyI GRCGYC 1 cut(s) 767
AfaI GTAC 1 cut(s) 475
AfiI CCNNNNNNNGG 3 cut(s) 41, 185, 263
AgsI TTSAA 6 cut(s) 17, 293, 302, 379, 401, 905
AluBI AGCT 7 cut(s) 70, 316, 395, 511, 578, 590, 845
AluI AGCT 7 cut(s) 70, 316, 395, 511, 578, 590, 845
AoxI GGCC 2 cut(s) 367, 828
ApeKI GCWGC 1 cut(s) 704
ApoI RAATTY 2 cut(s) 9, 534
BauI CACGAG 1 cut(s) 390
BbsI GAAGAC 1 cut(s) 318
BbvI GCAGC 1 cut(s) 691
BccI CCATC 4 cut(s) 460, 524, 655, 842
BciVI GTATCC 3 cut(s) 153, 542, 863
BclI TGATCA 1 cut(s) 748
BfaI CTAG 3 cut(s) 77, 425, 543
BfuI GTATCC 3 cut(s) 153, 542, 863
BglII AGATCT 1 cut(s) 129
BisI GCNGC 4 cut(s) 486, 702, 705, 828
BlsI GCNGC 4 cut(s) 487, 703, 706, 829
BmsI GCATC 3 cut(s) 138, 373, 695
BpiI GAAGAC 1 cut(s) 318
BsaAI YACGTR 1 cut(s) 164
BsaHI GRCGYC 1 cut(s) 767
BsaJI CCNNGG 1 cut(s) 370
BsaWI WCCGGW 1 cut(s) 770
Bsc4I CCNNNNNNNGG 3 cut(s) 41, 185, 263
Bse1I ACTGG 2 cut(s) 261, 729
BseDI CCNNGG 1 cut(s) 370
BseGI GGATG 2 cut(s) 491, 686
BseLI CCNNNNNNNGG 3 cut(s) 41, 185, 263
BseMII CTCAG 1 cut(s) 308
BseNI ACTGG 2 cut(s) 261, 729
BseRI GAGGAG 1 cut(s) 60
BseXI GCAGC 1 cut(s) 691
BshFI GGCC 2 cut(s) 369, 830
BsiSI CCGG 1 cut(s) 771
BslI CCNNNNNNNGG 3 cut(s) 41, 185, 263
BsmI GAATGC 1 cut(s) 339
BsnI GGCC 2 cut(s) 369, 830
Bsp143I GATC 3 cut(s) 129, 622, 748
BspACI CCGC 8 cut(s) 483, 486, 501, 690, 699, 702, 740, 827
BspANI GGCC 2 cut(s) 369, 830
BspCNI CTCAG 1 cut(s) 309
BspHI TCATGA 1 cut(s) 139
BspQI GCTCTTC 1 cut(s) 306
BsrI ACTGG 2 cut(s) 261, 729
BssECI CCNNGG 1 cut(s) 370
BssMI GATC 3 cut(s) 129, 622, 748
BssNAI GTATAC 3 cut(s) 176, 584, 896
BssNI GRCGYC 1 cut(s) 767
BssSI CACGAG 1 cut(s) 390
Bst1107I GTATAC 3 cut(s) 176, 584, 896
Bst2BI CACGAG 1 cut(s) 390
Bst4CI ACNGT 3 cut(s) 180, 601, 911
Bst6I CTCTTC 1 cut(s) 306
BstACI GRCGYC 1 cut(s) 767
BstBAI YACGTR 1 cut(s) 164
BstC8I GCNNGC 2 cut(s) 72, 153
BstDEI CTNAG 2 cut(s) 317, 619
BstF5I GGATG 2 cut(s) 491, 686
BstKTI GATC 3 cut(s) 132, 625, 751
BstMBI GATC 3 cut(s) 129, 622, 748
BstMWI GCNNNNNNNGC 2 cut(s) 392, 698
BstNSI RCATGY 3 cut(s) 155, 480, 675
BstV1I GCAGC 1 cut(s) 691
BstV2I GAAGAC 1 cut(s) 318
BstX2I RGATCY 1 cut(s) 129
BstYI RGATCY 1 cut(s) 129
BstZ17I GTATAC 3 cut(s) 176, 584, 896
BsuI GTATCC 3 cut(s) 153, 542, 863
BsuRI GGCC 2 cut(s) 369, 830
BtsCI GGATG 2 cut(s) 491, 686
BtsIMutI CAGTG 3 cut(s) 268, 350, 736
Cac8I GCNNGC 2 cut(s) 72, 153
CciI TCATGA 1 cut(s) 139
CseI GACGC 2 cut(s) 90, 756
Csp6I GTAC 1 cut(s) 474
CspCI CAANNNNNGTGG 2 cut(s) 790, 825
CviAII CATG 6 cut(s) 140, 152, 156, 477, 672, 752
CviQI GTAC 1 cut(s) 474
DdeI CTNAG 2 cut(s) 317, 619
DpnI GATC 3 cut(s) 131, 624, 750
DpnII GATC 3 cut(s) 129, 622, 748
DraI TTTAAA 1 cut(s) 415
EaeI YGGCCR 2 cut(s) 367, 828
Eam1104I CTCTTC 1 cut(s) 306
EarI CTCTTC 1 cut(s) 306
EciI GGCGGA 3 cut(s) 472, 679, 688
Eco32I GATATC 1 cut(s) 52
Eco57I CTGAAG 2 cut(s) 228, 312
EcoRI GAATTC 2 cut(s) 9, 534
EcoRV GATATC 1 cut(s) 52
EcoT22I ATGCAT 2 cut(s) 153, 157
FaeI CATG 6 cut(s) 143, 155, 159, 480, 675, 755
FatI CATG 6 cut(s) 139, 151, 155, 476, 671, 751
FauI CCCGC 1 cut(s) 747
FbaI TGATCA 1 cut(s) 748
FblI GTMKAC 4 cut(s) 175, 583, 646, 895
Fnu4HI GCNGC 4 cut(s) 486, 702, 705, 828
FokI GGATG 2 cut(s) 478, 673
Fsp4HI GCNGC 4 cut(s) 486, 702, 705, 828
FspBI CTAG 3 cut(s) 77, 425, 543
GluI GCNGC 4 cut(s) 486, 702, 705, 828
HaeIII GGCC 2 cut(s) 369, 830
HapII CCGG 1 cut(s) 771
HgaI GACGC 2 cut(s) 90, 756
Hin1I GRCGYC 1 cut(s) 767
Hin1II CATG 6 cut(s) 143, 155, 159, 480, 675, 755
HincII GTYRAC 1 cut(s) 714
HindII GTYRAC 1 cut(s) 714
HinfI GANTC 2 cut(s) 323, 375
HpaI GTTAAC 1 cut(s) 714
HpaII CCGG 1 cut(s) 771
Hpy166II GTNNAC 6 cut(s) 167, 176, 584, 647, 714, 896
Hpy188I TCNGA 2 cut(s) 36, 566
Hpy188III TCNNGA 2 cut(s) 140, 543
Hpy8I GTNNAC 6 cut(s) 167, 176, 584, 647, 714, 896
Hpy99I CGWCG 1 cut(s) 227
HpyAV CCTTC 3 cut(s) 206, 267, 817
HpyCH4III ACNGT 3 cut(s) 180, 601, 911
HpyCH4IV ACGT 2 cut(s) 163, 225
HpyCH4V TGCA 3 cut(s) 151, 155, 762
HpyF10VI GCNNNNNNNGC 2 cut(s) 392, 698
HpyF3I CTNAG 2 cut(s) 317, 619
HpySE526I ACGT 2 cut(s) 163, 225
Hsp92I GRCGYC 1 cut(s) 767
Hsp92II CATG 6 cut(s) 143, 155, 159, 480, 675, 755
Ksp22I TGATCA 1 cut(s) 748
KspAI GTTAAC 1 cut(s) 714
Kzo9I GATC 3 cut(s) 129, 622, 748
LguI GCTCTTC 1 cut(s) 306
LmnI GCTCC 2 cut(s) 485, 680
LpnPI CCDG 7 cut(s) 123, 274, 497, 564, 742, 759, 784
Lsp1109I GCAGC 1 cut(s) 691
LweI GCATC 3 cut(s) 138, 373, 695
MaeI CTAG 3 cut(s) 77, 425, 543
MaeII ACGT 2 cut(s) 163, 225
MaeIII GTNAC 1 cut(s) 347
MalI GATC 3 cut(s) 131, 624, 750
MboI GATC 3 cut(s) 129, 622, 748
MboII GAAGA 4 cut(s) 5, 221, 318, 323
MfeI CAATTG 1 cut(s) 807
MflI RGATCY 1 cut(s) 129
MluCI AATT 5 cut(s) 9, 119, 534, 807, 900
MlyI GAGTC 1 cut(s) 332
MmeI TCCRAC 1 cut(s) 225
MnlI CCTC 8 cut(s) 38, 182, 352, 365, 499, 539, 706, 884
Mph1103I ATGCAT 2 cut(s) 153, 157
MseI TTAA 3 cut(s) 414, 438, 713
MslI CAYNNNNRTG 1 cut(s) 238
MspA1I CMGCKG 1 cut(s) 704
MspI CCGG 1 cut(s) 771
MunI CAATTG 1 cut(s) 807
Mva1269I GAATGC 1 cut(s) 339
MwoI GCNNNNNNNGC 2 cut(s) 392, 698
NdeII GATC 3 cut(s) 129, 622, 748
NlaIII CATG 6 cut(s) 143, 155, 159, 480, 675, 755
NmeAIII GCCGAG 2 cut(s) 395, 733
NsiI ATGCAT 2 cut(s) 153, 157
NspI RCATGY 3 cut(s) 155, 480, 675
PaeI GCATGC 1 cut(s) 155
PagI TCATGA 1 cut(s) 139
PciSI GCTCTTC 1 cut(s) 306
PctI GAATGC 1 cut(s) 339
PfeI GAWTC 1 cut(s) 375
PkrI GCNGC 4 cut(s) 487, 703, 706, 829
PleI GAGTC 1 cut(s) 331
PpsI GAGTC 1 cut(s) 331
Ppu21I YACGTR 1 cut(s) 164
PsuI RGATCY 1 cut(s) 129
RsaI GTAC 1 cut(s) 475
RsaNI GTAC 1 cut(s) 474
RseI CAYNNNNRTG 1 cut(s) 238
SapI GCTCTTC 1 cut(s) 306
SaqAI TTAA 3 cut(s) 414, 438, 713
SatI GCNGC 4 cut(s) 486, 702, 705, 828
Sau3AI GATC 3 cut(s) 129, 622, 748
SchI GAGTC 1 cut(s) 332
SfaNI GCATC 3 cut(s) 138, 373, 695
SmiMI CAYNNNNRTG 1 cut(s) 238
SphI GCATGC 1 cut(s) 155
Sse9I AATT 5 cut(s) 9, 119, 534, 807, 900
SsiI CCGC 8 cut(s) 483, 486, 501, 690, 699, 702, 740, 827
SspMI CTAG 3 cut(s) 77, 425, 543
TaaI ACNGT 3 cut(s) 180, 601, 911
TaiI ACGT 2 cut(s) 166, 228
TaqI TCGA 1 cut(s) 270
TaqII GACCGA 1 cut(s) 528
TasI AATT 5 cut(s) 9, 119, 534, 807, 900
TatI WGTACW 1 cut(s) 473
TauI GCSGC 3 cut(s) 488, 704, 830
TfiI GAWTC 1 cut(s) 375
Tru1I TTAA 3 cut(s) 414, 438, 713
Tru9I TTAA 3 cut(s) 414, 438, 713
TscAI CASTG 3 cut(s) 268, 350, 736
TseI GCWGC 1 cut(s) 704
TspDTI ATGAA 4 cut(s) 424, 562, 805, 878
TspRI CASTG 3 cut(s) 268, 350, 736
XapI RAATTY 2 cut(s) 9, 534
XbaI TCTAGA 1 cut(s) 542
XceI RCATGY 3 cut(s) 155, 480, 675
XmiI GTMKAC 4 cut(s) 175, 583, 646, 895
XspI CTAG 3 cut(s) 77, 425, 543
Zsp2I ATGCAT 2 cut(s) 153, 157
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.