Rh7AG120400

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr7A
OG0000415
Physical Location & Seq
Reverse (-)
9416254 .. 9428966
12713 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh7AG120400.1

Sequence Viewer

Length: 1329 bp
ATGTCGCAGATTAACGACTCTGAGGAAGTGAAGCATGATACTAGTTTGGCAAACGCTTCCGATCCATGGTGTAATACTTGCTTCAGATATAAGGAAGCTGGAGAAACAGGATGTCTGAACCTTGGTGATGGCCGACAACTTTGCTCAGATTGTTCTTCTATAGCGGTCACGAATTTAGAGGAATGCAATTTCATAATCCAAAATGTGTTTGAATTCTACAGAATCTTAAATCTCAAAGTGGATGAAAAAATTCCCATCTTCTTCGTCGACAAGATTCAAATGAGCAGAATATCTCAAGATGGTTCAGTTGCTATCTTCATTCCAAGAATATCGGCTTACTTTACACCGTTAACGGCTTGCATACCATTACGAGTTAAAATGATGCTTATTTTAGCCAGATTTATTATACGCAGTATTCAAACGATAGAAAAATATTCCGGAAGTGGAGGTGGTATTAAAAAGGTTGTGAAGCTCCCACTAGGAGAGGCAATTGTACTTTTGTTTGGAACACCAAGTATAGAAATGGGAGCAACCTTGGCACATGAGATGATGCATGGATGGTTCCGCCTTCAAGGTATGCCATGGGGATGTGAAAATAGGACTGAAGAAGGTATTTGCGAAGTAATGTCTTACAAATGGTTGCAGTGGTTTTCTTCCAGTGGTCTTGATACCTCGCACAAAACCATACAGCAAGTTCAATATACAAGAAAGCTGAAAGAGTTCTTAGTAGAAGAAATTCGGTGTCGAGAAGACGAAGTTTATGGCCAAGGATTCAGAGATGCTATGCATGCTGTTGAAACATTTGGCTTTAAAACCACACTAGACCATGCCGTTAAAAATGGAACTCTACCACTACCTATGATGGATAGTGCACGCTCCACCGCCACAGCTGCCGCCGTCACCACTACCACCAAGAAGTTAATAAGAAAGAATACCAGTGCCACCCCAATAACTCGGTTATTATGGAAGGCGACCAGCAATACAACCAAGTCATTATGGAAGGCCACAGCTGCCGCAATAACTGACTTATTATGGAAGGCGGCCACCACCTGCGAAACGCCGCGGTTTATTATTATGGAAGGTCACTGCCACAACCAATATCATCAAGGGCAAAGCCATAAAAACTTACAAGAAGGCATGTCCTTGTCCCCTATGCTCCTTGTATTTATTGGTACTTCCAGTAAAATAACACCATTGCAGCTGGAAGGCTTGCTGACCGAAGGGAGCACATCAAGACTTTGGGTCGGTTCTGCCAGTTTCTCGGTTCCTAAACATGGTACCAGAAGTCCAGAACAGGTAAAAGTCAAAACAGCCAAAATGCAAAACTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

442

Amino Acids

49.4

Weight (kDa)

8.73

Isoelectric Point (pI)

41.53

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 162 - 277 3.4e-30 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1058
Acc36I ACCTGC 1 cut(s) 1058
Acc65I GGTACC 1 cut(s) 1277
AccB1I GGYRCC 1 cut(s) 1277
AccI GTMKAC 1 cut(s) 267
AccII CGCG 1 cut(s) 1063
AccIII TCCGGA 1 cut(s) 437
AciI CCGC 8 cut(s) 164, 565, 882, 894, 1014, 1040, 1061, 1063
AclWI GGATC 1 cut(s) 56
AcoI YGGCCR 3 cut(s) 130, 763, 1041
AcsI RAATTY 4 cut(s) 172, 212, 249, 735
AcuI CTGAAG 2 cut(s) 67, 624
AfaI GTAC 3 cut(s) 495, 1174, 1279
AfiI CCNNNNNNNGG 2 cut(s) 66, 1274
AgsI TTSAA 6 cut(s) 212, 278, 419, 572, 698, 797
AhdI GACNNNNNGTC 1 cut(s) 1241
AhlI ACTAGT 1 cut(s) 41
AluBI AGCT 6 cut(s) 98, 472, 712, 890, 1010, 1201
AluI AGCT 6 cut(s) 98, 472, 712, 890, 1010, 1201
Alw21I GWGCWC 2 cut(s) 874, 1229
Alw44I GTGCAC 1 cut(s) 870
AlwI GGATC 1 cut(s) 56
Aor13HI TCCGGA 1 cut(s) 437
AoxI GGCC 4 cut(s) 130, 763, 1002, 1041
ApaLI GTGCAC 1 cut(s) 870
ApeKI GCWGC 3 cut(s) 890, 1010, 1198
ApoI RAATTY 4 cut(s) 172, 212, 249, 735
Asp700I GAANNNNTTC 3 cut(s) 249, 719, 735
Asp718I GGTACC 1 cut(s) 1277
AsuHPI GGTGA 2 cut(s) 137, 892
BaeGI GKGCMC 1 cut(s) 874
BalI TGGCCA 1 cut(s) 765
BanI GGYRCC 1 cut(s) 1277
BbsI GAAGAC 1 cut(s) 756
Bbv12I GWGCWC 2 cut(s) 874, 1229
BbvI GCAGC 3 cut(s) 877, 997, 1210
BccI CCATC 5 cut(s) 122, 263, 293, 552, 856
BceAI ACGGC 3 cut(s) 369, 815, 881
BcgI CGANNNNNNTGC 2 cut(s) 123, 157
BcuI ACTAGT 1 cut(s) 41
BfaI CTAG 3 cut(s) 42, 479, 821
BfmI CTRYAG 2 cut(s) 159, 217
BfuAI ACCTGC 1 cut(s) 1058
BisI GCNGC 7 cut(s) 891, 894, 1011, 1014, 1041, 1061, 1199
BlsI GCNGC 7 cut(s) 892, 895, 1012, 1015, 1042, 1062, 1200
BmeRI GACNNNNNGTC 1 cut(s) 1241
BmiI GGNNCC 3 cut(s) 563, 1266, 1279
BmsI GCATC 3 cut(s) 372, 540, 769
BpiI GAAGAC 1 cut(s) 756
BpmI CTGGAG 1 cut(s) 120
BpuEI CTTGAG 1 cut(s) 279
BsaJI CCNNGG 6 cut(s) 65, 121, 534, 581, 766, 1061
BsaWI WCCGGW 1 cut(s) 437
Bsc4I CCNNNNNNNGG 2 cut(s) 66, 1274
Bse1I ACTGG 4 cut(s) 657, 936, 1179, 1254
Bse3DI GCAATG 1 cut(s) 1193
BseAI TCCGGA 1 cut(s) 437
BseDI CCNNGG 6 cut(s) 65, 121, 534, 581, 766, 1061
BseGI GGATG 4 cut(s) 116, 247, 563, 593
BseLI CCNNNNNNNGG 2 cut(s) 66, 1274
BseMI GCAATG 1 cut(s) 1193
BseMII CTCAG 2 cut(s) 12, 159
BseNI ACTGG 4 cut(s) 657, 936, 1179, 1254
BseSI GKGCMC 1 cut(s) 874
BseXI GCAGC 3 cut(s) 877, 997, 1210
Bsh1236I CGCG 1 cut(s) 1063
BshFI GGCC 4 cut(s) 132, 765, 1004, 1043
BshNI GGYRCC 1 cut(s) 1277
BsiHKAI GWGCWC 2 cut(s) 874, 1229
BsiSI CCGG 1 cut(s) 438
BslFI GGGAC 1 cut(s) 1132
BslI CCNNNNNNNGG 2 cut(s) 66, 1274
BsmFI GGGAC 1 cut(s) 1132
BsmI GAATGC 1 cut(s) 188
BsnI GGCC 4 cut(s) 132, 765, 1004, 1043
Bsp1286I GDGCHC 2 cut(s) 874, 1229
Bsp13I TCCGGA 1 cut(s) 437
Bsp143I GATC 1 cut(s) 61
Bsp19I CCATGG 2 cut(s) 65, 581
BspACI CCGC 8 cut(s) 164, 565, 882, 894, 1014, 1040, 1061, 1063
BspANI GGCC 4 cut(s) 132, 765, 1004, 1043
BspCNI CTCAG 2 cut(s) 13, 158
BspEI TCCGGA 1 cut(s) 437
BspFNI CGCG 1 cut(s) 1063
BspLI GGNNCC 3 cut(s) 563, 1266, 1279
BspMI ACCTGC 1 cut(s) 1058
BspPI GGATC 1 cut(s) 56
BspT107I GGYRCC 1 cut(s) 1277
BsrDI GCAATG 1 cut(s) 1193
BsrI ACTGG 4 cut(s) 657, 936, 1179, 1254
BssECI CCNNGG 6 cut(s) 65, 121, 534, 581, 766, 1061
BssMI GATC 1 cut(s) 61
BssT1I CCWWGG 5 cut(s) 65, 121, 534, 581, 766
Bst4CI ACNGT 1 cut(s) 348
BstC8I GCNNGC 4 cut(s) 358, 789, 874, 1211
BstDEI CTNAG 3 cut(s) 21, 145, 724
BstDSI CCRYGG 3 cut(s) 65, 581, 1061
BstF5I GGATG 4 cut(s) 116, 247, 563, 593
BstFNI CGCG 1 cut(s) 1063
BstKTI GATC 1 cut(s) 64
BstMBI GATC 1 cut(s) 61
BstMWI GCNNNNNNNGC 4 cut(s) 536, 788, 890, 1010
BstNSI RCATGY 2 cut(s) 791, 1141
BstSFI CTRYAG 2 cut(s) 159, 217
BstSLI GKGCMC 1 cut(s) 874
BstUI CGCG 1 cut(s) 1063
BstV1I GCAGC 3 cut(s) 877, 997, 1210
BstV2I GAAGAC 1 cut(s) 756
BsuRI GGCC 4 cut(s) 132, 765, 1004, 1043
BtgI CCRYGG 3 cut(s) 65, 581, 1061
BtsCI GGATG 4 cut(s) 116, 247, 563, 593
BtsI GCAGTG 2 cut(s) 650, 1084
BtsIMutI CAGTG 4 cut(s) 650, 664, 943, 1084
BveI ACCTGC 1 cut(s) 1058
Cac8I GCNNGC 4 cut(s) 358, 789, 874, 1211
Cfr42I CCGCGG 1 cut(s) 1064
Csp6I GTAC 3 cut(s) 494, 1173, 1278
CviAII CATG 9 cut(s) 35, 66, 542, 554, 582, 788, 827, 1138, 1274
CviQI GTAC 3 cut(s) 494, 1173, 1278
DdeI CTNAG 3 cut(s) 21, 145, 724
DpnI GATC 1 cut(s) 63
DpnII GATC 1 cut(s) 61
DraI TTTAAA 1 cut(s) 811
DriI GACNNNNNGTC 1 cut(s) 1241
EaeI YGGCCR 3 cut(s) 130, 763, 1041
Eam1105I GACNNNNNGTC 1 cut(s) 1241
EciI GGCGGA 1 cut(s) 554
Eco130I CCWWGG 5 cut(s) 65, 121, 534, 581, 766
Eco57I CTGAAG 2 cut(s) 67, 624
EcoRI GAATTC 1 cut(s) 212
EcoT14I CCWWGG 5 cut(s) 65, 121, 534, 581, 766
EcoT22I ATGCAT 2 cut(s) 555, 789
ErhI CCWWGG 5 cut(s) 65, 121, 534, 581, 766
FaeI CATG 9 cut(s) 38, 69, 545, 557, 585, 791, 830, 1141, 1277
FaqI GGGAC 1 cut(s) 1132
FatI CATG 9 cut(s) 34, 65, 541, 553, 581, 787, 826, 1137, 1273
FblI GTMKAC 1 cut(s) 267
Fnu4HI GCNGC 7 cut(s) 891, 894, 1011, 1014, 1041, 1061, 1199
FokI GGATG 4 cut(s) 123, 254, 570, 600
Fsp4HI GCNGC 7 cut(s) 891, 894, 1011, 1014, 1041, 1061, 1199
FspBI CTAG 3 cut(s) 42, 479, 821
GluI GCNGC 7 cut(s) 891, 894, 1011, 1014, 1041, 1061, 1199
GsuI CTGGAG 1 cut(s) 120
HaeIII GGCC 4 cut(s) 132, 765, 1004, 1043
HapII CCGG 1 cut(s) 438
Hin1II CATG 9 cut(s) 38, 69, 545, 557, 585, 791, 830, 1141, 1277
HincII GTYRAC 2 cut(s) 268, 351
HindII GTYRAC 2 cut(s) 268, 351
HinfI GANTC 4 cut(s) 17, 222, 274, 771
HpaI GTTAAC 1 cut(s) 351
HpaII CCGG 1 cut(s) 438
HphI GGTGA 2 cut(s) 137, 892
Hpy166II GTNNAC 3 cut(s) 268, 351, 872
Hpy188I TCNGA 6 cut(s) 22, 61, 86, 117, 148, 776
Hpy188III TCNNGA 7 cut(s) 169, 296, 438, 665, 746, 1233, 1289
Hpy8I GTNNAC 3 cut(s) 268, 351, 872
Hpy99I CGWCG 1 cut(s) 269
HpyAV CCTTC 9 cut(s) 578, 602, 961, 994, 1030, 1073, 1127, 1199, 1214
HpyCH4III ACNGT 1 cut(s) 348
HpyCH4V TGCA 8 cut(s) 186, 360, 553, 643, 787, 872, 1198, 1321
HpyF10VI GCNNNNNNNGC 4 cut(s) 536, 788, 890, 1010
HpyF3I CTNAG 3 cut(s) 21, 145, 724
Hsp92II CATG 9 cut(s) 38, 69, 545, 557, 585, 791, 830, 1141, 1277
Kpn2I TCCGGA 1 cut(s) 437
KpnI GGTACC 1 cut(s) 1281
KspAI GTTAAC 1 cut(s) 351
KspI CCGCGG 1 cut(s) 1064
Kzo9I GATC 1 cut(s) 61
LmnI GCTCC 5 cut(s) 477, 527, 881, 1161, 1224
Lsp1109I GCAGC 3 cut(s) 877, 997, 1210
LweI GCATC 3 cut(s) 372, 540, 769
MaeI CTAG 3 cut(s) 42, 479, 821
MaeIII GTNAC 3 cut(s) 166, 898, 1082
MalI GATC 1 cut(s) 63
MboI GATC 1 cut(s) 61
MboII GAAGA 8 cut(s) 147, 250, 253, 307, 617, 645, 743, 761
MfeI CAATTG 1 cut(s) 489
MhlI GDGCHC 2 cut(s) 874, 1229
MlsI TGGCCA 1 cut(s) 765
MluCI AATT 6 cut(s) 172, 187, 212, 249, 489, 735
MluNI TGGCCA 1 cut(s) 765
MlyI GAGTC 1 cut(s) 11
MnlI CCTC 5 cut(s) 16, 172, 440, 478, 682
Mox20I TGGCCA 1 cut(s) 765
Mph1103I ATGCAT 2 cut(s) 555, 789
MroI TCCGGA 1 cut(s) 437
MroXI GAANNNNTTC 3 cut(s) 249, 719, 735
MscI TGGCCA 1 cut(s) 765
MseI TTAA 8 cut(s) 12, 227, 350, 375, 456, 810, 834, 920
MslI CAYNNNNRTG 1 cut(s) 586
Msp20I TGGCCA 1 cut(s) 765
MspA1I CMGCKG 4 cut(s) 890, 1010, 1063, 1201
MspI CCGG 1 cut(s) 438
MunI CAATTG 1 cut(s) 489
Mva1269I GAATGC 1 cut(s) 188
MvnI CGCG 1 cut(s) 1063
MwoI GCNNNNNNNGC 4 cut(s) 536, 788, 890, 1010
NcoI CCATGG 2 cut(s) 65, 581
NdeII GATC 1 cut(s) 61
NlaIII CATG 9 cut(s) 38, 69, 545, 557, 585, 791, 830, 1141, 1277
NlaIV GGNNCC 3 cut(s) 563, 1266, 1279
NmuCI GTSAC 3 cut(s) 166, 898, 1082
NsiI ATGCAT 2 cut(s) 555, 789
NspI RCATGY 2 cut(s) 791, 1141
PaeI GCATGC 1 cut(s) 791
PaqCI CACCTGC 1 cut(s) 1058
PctI GAATGC 1 cut(s) 188
PdmI GAANNNNTTC 3 cut(s) 249, 719, 735
PfeI GAWTC 3 cut(s) 222, 274, 771
PkrI GCNGC 7 cut(s) 892, 895, 1012, 1015, 1042, 1062, 1200
PleI GAGTC 1 cut(s) 11
PpsI GAGTC 1 cut(s) 11
PspN4I GGNNCC 3 cut(s) 563, 1266, 1279
PvuII CAGCTG 3 cut(s) 890, 1010, 1201
RsaI GTAC 3 cut(s) 495, 1174, 1279
RsaNI GTAC 3 cut(s) 494, 1173, 1278
RseI CAYNNNNRTG 1 cut(s) 586
SacII CCGCGG 1 cut(s) 1064
SalI GTCGAC 1 cut(s) 266
SaqAI TTAA 8 cut(s) 12, 227, 350, 375, 456, 810, 834, 920
SatI GCNGC 7 cut(s) 891, 894, 1011, 1014, 1041, 1061, 1199
Sau3AI GATC 1 cut(s) 61
SchI GAGTC 1 cut(s) 11
SduI GDGCHC 2 cut(s) 874, 1229
SfaNI GCATC 3 cut(s) 372, 540, 769
SfcI CTRYAG 2 cut(s) 159, 217
Sfr303I CCGCGG 1 cut(s) 1064
SgrBI CCGCGG 1 cut(s) 1064
SmiMI CAYNNNNRTG 1 cut(s) 586
SmlI CTYRAG 1 cut(s) 294
SmoI CTYRAG 1 cut(s) 294
SpeI ACTAGT 1 cut(s) 41
SphI GCATGC 1 cut(s) 791
Sse9I AATT 6 cut(s) 172, 187, 212, 249, 489, 735
SsiI CCGC 8 cut(s) 164, 565, 882, 894, 1014, 1040, 1061, 1063
SspI AATATT 1 cut(s) 434
SspMI CTAG 3 cut(s) 42, 479, 821
StyI CCWWGG 5 cut(s) 65, 121, 534, 581, 766
TaaI ACNGT 1 cut(s) 348
TaqI TCGA 2 cut(s) 267, 745
TaqII GACCGA 1 cut(s) 1232
TasI AATT 6 cut(s) 172, 187, 212, 249, 489, 735
TatI WGTACW 1 cut(s) 493
TauI GCSGC 4 cut(s) 896, 1016, 1043, 1063
TfiI GAWTC 3 cut(s) 222, 274, 771
Tru1I TTAA 8 cut(s) 12, 227, 350, 375, 456, 810, 834, 920
Tru9I TTAA 8 cut(s) 12, 227, 350, 375, 456, 810, 834, 920
TscAI CASTG 4 cut(s) 650, 664, 943, 1091
TseFI GTSAC 3 cut(s) 166, 898, 1082
TseI GCWGC 3 cut(s) 890, 1010, 1198
Tsp45I GTSAC 3 cut(s) 166, 898, 1082
TspDTI ATGAA 3 cut(s) 181, 258, 307
TspRI CASTG 4 cut(s) 650, 664, 943, 1091
VneI GTGCAC 1 cut(s) 870
XapI RAATTY 4 cut(s) 172, 212, 249, 735
XceI RCATGY 2 cut(s) 791, 1141
XmiI GTMKAC 1 cut(s) 267
XmnI GAANNNNTTC 3 cut(s) 249, 719, 735
XspI CTAG 3 cut(s) 42, 479, 821
Zsp2I ATGCAT 2 cut(s) 555, 789
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.