Rh6DG257600

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr6D
OG0000415
Physical Location & Seq
Reverse (-)
44792632 .. 44795124
2493 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh6DG257600.1

Sequence Viewer

Length: 873 bp
ATGACTAATTCCAATAGCTGCGGTGCCAATTTCAACAATCCAGGATGCAGAGACAGCATGAATATTTGTTACGTTCGCAAAAAACATTCTAACTCATTGGCTCATGATAAATTTTGGGACCAAAGATGCTGTTCTGGACATTACTTTGACGGAACTCTGAATTGTATCACTTGTCATAAATTTAAGCAAGCCGGGGAGATAGAATACTTGAACCATGGTCGTGGTCTAATGACTTGTTCAAAATGCTGTTCTATTGCTATTAAGAATTCAGAAGAATTCAAGCCAATTATTGAAAATGTGCATAATTTTTATGACAGTTTAAATCTGAAGGTTGATCGAGACATCCCTATTTTGTTGGTCGATGGTAATGAGATGTTCGGATCTAAACAAGATTTTGACTCGGTATATTTCAGTATAGCCATGGGAGGAACTTTAGCACATGAGATGATGCATGCTTGGTTGAAACTTCAAGGTTGTAAGCCGTGGGAATGGGAAAGAAGAGTTGAAGAAGGGATCTGCCAAGTAATGAAGTACAAGTGGATGGAATGGTTTTGTTCTAGTAGTGATTTTGACTCTTGGTACAAAACCAATGAGCAAGCTCAATACGCAAGAAAGTTGAAAGAGTATCAAGTCCAAGTGATGGAGAGGTGGGTAGATAAAGTTTATGGCCAAGGGTTCAGAGATGCTATGAAGGCTGTAGAAATGTTTGGCTTCGAAACCACGCTGCACTACATCGTTGAAAAAGGAAAATTCCCTCGCAGTCAACCACCTCCTGTCCCGATTCCTAGGACACCTAGGGATGAAAGATGGAAGGGGGCTGTATGCGCTGTAGCTCATATAGCTCTTGTTTTTCTTTTAATGAAGGCTAGATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

290

Amino Acids

33.75

Weight (kDa)

8.32

Isoelectric Point (pI)

36.39

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 139 - 246 9.1e-25 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 23
AccB7I CCANNNNNTGG 1 cut(s) 640
AciI CCGC 1 cut(s) 21
AclWI GGATC 2 cut(s) 388, 521
AcoI YGGCCR 1 cut(s) 667
AcsI RAATTY 5 cut(s) 110, 179, 265, 275, 749
AcuI CTGAAG 1 cut(s) 347
AfaI GTAC 2 cut(s) 533, 581
AfiI CCNNNNNNNGG 1 cut(s) 640
AjnI CCWGG 1 cut(s) 40
AluBI AGCT 4 cut(s) 18, 599, 833, 842
AluI AGCT 4 cut(s) 18, 599, 833, 842
Alw26I GTCTC 2 cut(s) 45, 333
AlwI GGATC 2 cut(s) 388, 521
AoxI GGCC 1 cut(s) 667
ApeKI GCWGC 2 cut(s) 18, 724
ApoI RAATTY 5 cut(s) 110, 179, 265, 275, 749
AspA2I CCTAGG 2 cut(s) 785, 794
AspLEI GCGC 1 cut(s) 827
AspS9I GGNCC 1 cut(s) 118
AsuC2I CCSGG 1 cut(s) 193
AsuII TTCGAA 1 cut(s) 714
AvaII GGWCC 1 cut(s) 118
AvrII CCTAGG 2 cut(s) 785, 794
BalI TGGCCA 1 cut(s) 669
BanI GGYRCC 1 cut(s) 23
BbvI GCAGC 2 cut(s) 5, 711
BccI CCATC 4 cut(s) 356, 535, 634, 801
BceAI ACGGC 1 cut(s) 466
BciT130I CCWGG 1 cut(s) 42
BcnI CCSGG 1 cut(s) 193
BcoDI GTCTC 2 cut(s) 45, 333
BfaI CTAG 4 cut(s) 558, 786, 795, 867
BfmI CTRYAG 2 cut(s) 696, 828
BisI GCNGC 2 cut(s) 19, 725
BlnI CCTAGG 2 cut(s) 785, 794
BlsI GCNGC 2 cut(s) 20, 726
Bme1390I CCNGG 2 cut(s) 42, 193
Bme18I GGWCC 1 cut(s) 118
BmgT120I GGNCC 1 cut(s) 118
BmiI GGNNCC 2 cut(s) 25, 119
BmrFI CCNGG 2 cut(s) 42, 193
BmsI GCATC 4 cut(s) 35, 116, 438, 673
Bpu14I TTCGAA 1 cut(s) 714
BpuMI CCSGG 1 cut(s) 193
BsaJI CCNNGG 7 cut(s) 192, 214, 420, 482, 670, 785, 794
Bsc4I CCNNNNNNNGG 1 cut(s) 640
BseBI CCWGG 1 cut(s) 42
BseDI CCNNGG 7 cut(s) 192, 214, 420, 482, 670, 785, 794
BseGI GGATG 4 cut(s) 50, 342, 546, 805
BseLI CCNNNNNNNGG 1 cut(s) 640
BseXI GCAGC 2 cut(s) 5, 711
BsgI GTGCAG 1 cut(s) 710
BshFI GGCC 1 cut(s) 669
BshNI GGYRCC 1 cut(s) 23
BsiSI CCGG 1 cut(s) 192
BslFI GGGAC 2 cut(s) 131, 761
BslI CCNNNNNNNGG 1 cut(s) 640
BsmAI GTCTC 2 cut(s) 45, 333
BsmFI GGGAC 2 cut(s) 131, 761
BsnI GGCC 1 cut(s) 669
Bsp119I TTCGAA 1 cut(s) 714
Bsp143I GATC 3 cut(s) 334, 380, 513
Bsp19I CCATGG 2 cut(s) 214, 420
BspACI CCGC 1 cut(s) 21
BspANI GGCC 1 cut(s) 669
BspHI TCATGA 1 cut(s) 103
BspLI GGNNCC 2 cut(s) 25, 119
BspPI GGATC 2 cut(s) 388, 521
BspT104I TTCGAA 1 cut(s) 714
BspT107I GGYRCC 1 cut(s) 23
BssECI CCNNGG 7 cut(s) 192, 214, 420, 482, 670, 785, 794
BssMI GATC 3 cut(s) 334, 380, 513
BssT1I CCWWGG 5 cut(s) 214, 420, 670, 785, 794
Bst2UI CCWGG 1 cut(s) 42
Bst4CI ACNGT 1 cut(s) 317
Bst6I CTCTTC 1 cut(s) 493
BstBI TTCGAA 1 cut(s) 714
BstC8I GCNNGC 3 cut(s) 189, 453, 597
BstDSI CCRYGG 3 cut(s) 214, 420, 482
BstF5I GGATG 4 cut(s) 50, 342, 546, 805
BstHHI GCGC 1 cut(s) 827
BstKTI GATC 3 cut(s) 337, 383, 516
BstMAI GTCTC 2 cut(s) 45, 333
BstMBI GATC 3 cut(s) 334, 380, 513
BstMWI GCNNNNNNNGC 5 cut(s) 54, 605, 692, 824, 839
BstNI CCWGG 1 cut(s) 42
BstNSI RCATGY 1 cut(s) 455
BstSCI CCNGG 2 cut(s) 40, 191
BstSFI CTRYAG 2 cut(s) 696, 828
BstV1I GCAGC 2 cut(s) 5, 711
BstX2I RGATCY 2 cut(s) 380, 513
BstXI CCANNNNNNTGG 1 cut(s) 221
BstYI RGATCY 2 cut(s) 380, 513
BsuRI GGCC 1 cut(s) 669
BtgI CCRYGG 3 cut(s) 214, 420, 482
BtsCI GGATG 4 cut(s) 50, 342, 546, 805
Cac8I GCNNGC 3 cut(s) 189, 453, 597
CciI TCATGA 1 cut(s) 103
CfoI GCGC 1 cut(s) 827
Cfr13I GGNCC 1 cut(s) 118
Csp6I GTAC 2 cut(s) 532, 580
CviAII CATG 6 cut(s) 58, 104, 215, 421, 440, 452
CviQI GTAC 2 cut(s) 532, 580
DpnI GATC 3 cut(s) 336, 382, 515
DpnII GATC 3 cut(s) 334, 380, 513
DraI TTTAAA 1 cut(s) 321
EaeI YGGCCR 1 cut(s) 667
Eam1104I CTCTTC 1 cut(s) 493
EarI CTCTTC 1 cut(s) 493
Eco130I CCWWGG 5 cut(s) 214, 420, 670, 785, 794
Eco47I GGWCC 1 cut(s) 118
Eco57I CTGAAG 1 cut(s) 347
EcoRI GAATTC 2 cut(s) 265, 275
EcoRII CCWGG 1 cut(s) 40
EcoT14I CCWWGG 5 cut(s) 214, 420, 670, 785, 794
EcoT22I ATGCAT 1 cut(s) 453
ErhI CCWWGG 5 cut(s) 214, 420, 670, 785, 794
FaeI CATG 6 cut(s) 61, 107, 218, 424, 443, 455
FaqI GGGAC 2 cut(s) 131, 761
FatI CATG 6 cut(s) 57, 103, 214, 420, 439, 451
Fnu4HI GCNGC 2 cut(s) 19, 725
FokI GGATG 4 cut(s) 57, 329, 553, 812
Fsp4HI GCNGC 2 cut(s) 19, 725
FspBI CTAG 4 cut(s) 558, 786, 795, 867
GlaI GCGC 1 cut(s) 826
GluI GCNGC 2 cut(s) 19, 725
HaeIII GGCC 1 cut(s) 669
HapII CCGG 1 cut(s) 192
HhaI GCGC 1 cut(s) 827
Hin1II CATG 6 cut(s) 61, 107, 218, 424, 443, 455
Hin6I GCGC 1 cut(s) 825
HinP1I GCGC 1 cut(s) 825
HincII GTYRAC 1 cut(s) 764
HindII GTYRAC 1 cut(s) 764
HinfI GANTC 3 cut(s) 398, 572, 781
HpaII CCGG 1 cut(s) 192
Hpy166II GTNNAC 1 cut(s) 764
Hpy188I TCNGA 5 cut(s) 159, 271, 327, 380, 680
Hpy188III TCNNGA 4 cut(s) 104, 135, 338, 778
Hpy8I GTNNAC 1 cut(s) 764
HpyAV CCTTC 5 cut(s) 322, 503, 685, 805, 856
HpyCH4III ACNGT 1 cut(s) 317
HpyCH4IV ACGT 1 cut(s) 72
HpyCH4V TGCA 4 cut(s) 48, 301, 451, 727
HpyF10VI GCNNNNNNNGC 5 cut(s) 54, 605, 692, 824, 839
HpySE526I ACGT 1 cut(s) 72
Hsp92II CATG 6 cut(s) 61, 107, 218, 424, 443, 455
HspAI GCGC 1 cut(s) 825
Kzo9I GATC 3 cut(s) 334, 380, 513
LpnPI CCDG 5 cut(s) 27, 54, 120, 205, 786
Lsp1109I GCAGC 2 cut(s) 5, 711
LweI GCATC 4 cut(s) 35, 116, 438, 673
MaeI CTAG 4 cut(s) 558, 786, 795, 867
MaeII ACGT 1 cut(s) 72
MaeIII GTNAC 1 cut(s) 68
MalI GATC 3 cut(s) 336, 382, 515
MboI GATC 3 cut(s) 334, 380, 513
MboII GAAGA 3 cut(s) 284, 510, 518
MflI RGATCY 2 cut(s) 380, 513
MlsI TGGCCA 1 cut(s) 669
MluNI TGGCCA 1 cut(s) 669
MlyI GAGTC 2 cut(s) 392, 566
MnlI CCTC 4 cut(s) 419, 639, 765, 780
Mox20I TGGCCA 1 cut(s) 669
Mph1103I ATGCAT 1 cut(s) 453
MscI TGGCCA 1 cut(s) 669
MseI TTAA 4 cut(s) 183, 261, 320, 857
MslI CAYNNNNRTG 1 cut(s) 219
Msp20I TGGCCA 1 cut(s) 669
MspI CCGG 1 cut(s) 192
MspR9I CCNGG 2 cut(s) 42, 193
MvaI CCWGG 1 cut(s) 42
MwoI GCNNNNNNNGC 5 cut(s) 54, 605, 692, 824, 839
NciI CCSGG 1 cut(s) 193
NcoI CCATGG 2 cut(s) 214, 420
NdeII GATC 3 cut(s) 334, 380, 513
NlaIII CATG 6 cut(s) 61, 107, 218, 424, 443, 455
NlaIV GGNNCC 2 cut(s) 25, 119
NsiI ATGCAT 1 cut(s) 453
NspI RCATGY 1 cut(s) 455
NspV TTCGAA 1 cut(s) 714
PaeI GCATGC 1 cut(s) 455
PagI TCATGA 1 cut(s) 103
PfeI GAWTC 1 cut(s) 781
PflMI CCANNNNNTGG 1 cut(s) 640
PfoI TCCNGGA 1 cut(s) 40
PkrI GCNGC 2 cut(s) 20, 726
PleI GAGTC 2 cut(s) 392, 566
PpsI GAGTC 2 cut(s) 392, 566
Psp6I CCWGG 1 cut(s) 40
PspGI CCWGG 1 cut(s) 40
PspN4I GGNNCC 2 cut(s) 25, 119
PspPI GGNCC 1 cut(s) 118
PsuI RGATCY 2 cut(s) 380, 513
RsaI GTAC 2 cut(s) 533, 581
RsaNI GTAC 2 cut(s) 532, 580
RseI CAYNNNNRTG 1 cut(s) 219
SaqAI TTAA 4 cut(s) 183, 261, 320, 857
SatI GCNGC 2 cut(s) 19, 725
Sau3AI GATC 3 cut(s) 334, 380, 513
Sau96I GGNCC 1 cut(s) 118
SchI GAGTC 2 cut(s) 392, 566
ScrFI CCNGG 2 cut(s) 42, 193
SfaNI GCATC 4 cut(s) 35, 116, 438, 673
SfcI CTRYAG 2 cut(s) 696, 828
SfuI TTCGAA 1 cut(s) 714
SinI GGWCC 1 cut(s) 118
SmiMI CAYNNNNRTG 1 cut(s) 219
SphI GCATGC 1 cut(s) 455
SsiI CCGC 1 cut(s) 21
SspI AATATT 1 cut(s) 64
SspMI CTAG 4 cut(s) 558, 786, 795, 867
StyD4I CCNGG 2 cut(s) 40, 191
StyI CCWWGG 5 cut(s) 214, 420, 670, 785, 794
TaaI ACNGT 1 cut(s) 317
TaiI ACGT 1 cut(s) 75
TaqI TCGA 3 cut(s) 337, 360, 714
TatI WGTACW 1 cut(s) 531
TfiI GAWTC 1 cut(s) 781
Tru1I TTAA 4 cut(s) 183, 261, 320, 857
Tru9I TTAA 4 cut(s) 183, 261, 320, 857
TseI GCWGC 2 cut(s) 18, 724
TspDTI ATGAA 4 cut(s) 74, 542, 704, 816
TspGWI ACGGA 1 cut(s) 165
Van91I CCANNNNNTGG 1 cut(s) 640
VpaK11BI GGWCC 1 cut(s) 118
XapI RAATTY 5 cut(s) 110, 179, 265, 275, 749
XceI RCATGY 1 cut(s) 455
XmaJI CCTAGG 2 cut(s) 785, 794
XspI CTAG 4 cut(s) 558, 786, 795, 867
Zsp2I ATGCAT 1 cut(s) 453
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.