Rh2AG546400

Protein DA1

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0000415
Physical Location & Seq
Reverse (-)
77527946 .. 77528790
845 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG546400.1

Sequence Viewer

Length: 585 bp
ATGTTGGTCGACCAGGAAGAGATGTGCAAACGTTGCGGAGAAAAACCCAGTACAGAAGACGGTAATAGACGACTTTTTACTGGTGTTATATTTTTCCCTAGACGGCGTGCTCGTGTTCTCACGAGCATTACAAAATGTTCAAAAATTGGGGAAGATATCCAAGTGGAGAAAAAAACCGGCTCATCCCCGCGTAACAAAATTACAATTCTAGTTTTGTTCGGAGTACCAGACCTGTTGGTCGGTGGAACTTTGGCACACGAGATGATGCATGCATGGTTGCACCTTCAAGATTGTAGGAAAAAATTCGAAGAAAAGGTTGAAGAAGGGATTTGTGAAGTGATGTGCCACAAGTGGATGGAAAGGTTCTGTTCCAGTGATGATCTTGATCACTCCTCATACAAAACCTATAAGCAGGGCCAGTTTAAGAGAAAATTGAAACAATTTCTGGTAGAATCAATGGAAACCCGGCCAGACATTTATGGCCAAGGATACAGAGAGGCTACTAGGGCAGTTGAAAAGTTTGGCTTCCAAACGACTCTAAACCATATTGTTCAAAAAGAAAGTTTCCCTCACAGCGAAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DA1-like

Protein Analysis

194

Amino Acids

22.6

Weight (kDa)

8.79

Isoelectric Point (pI)

49.92

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DA1-like PF12315 38 - 184 4.7e-35 Protein DA1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 236
AccI GTMKAC 1 cut(s) 9
AccII CGCG 1 cut(s) 190
AciI CCGC 2 cut(s) 36, 188
AclI AACGTT 1 cut(s) 31
AcoI YGGCCR 2 cut(s) 467, 481
AcsI RAATTY 1 cut(s) 302
AfaI GTAC 2 cut(s) 52, 225
AgsI TTSAA 6 cut(s) 141, 287, 320, 436, 515, 554
AjnI CCWGG 1 cut(s) 12
Alw21I GWGCWC 1 cut(s) 112
AoxI GGCC 3 cut(s) 415, 467, 481
ApoI RAATTY 1 cut(s) 302
Asp700I GAANNNNTTC 1 cut(s) 302
AspS9I GGNCC 1 cut(s) 415
AsuC2I CCSGG 1 cut(s) 466
AsuII TTCGAA 1 cut(s) 306
BalI TGGCCA 1 cut(s) 483
BauI CACGAG 3 cut(s) 111, 121, 257
BbsI GAAGAC 1 cut(s) 63
Bbv12I GWGCWC 1 cut(s) 112
BccI CCATC 1 cut(s) 349
BceAI ACGGC 1 cut(s) 119
BciT130I CCWGG 1 cut(s) 14
BciVI GTATCC 1 cut(s) 482
BclI TGATCA 1 cut(s) 385
BcnI CCSGG 1 cut(s) 466
BfaI CTAG 3 cut(s) 99, 209, 504
BfuI GTATCC 1 cut(s) 482
Bme1390I CCNGG 2 cut(s) 14, 466
BmgT120I GGNCC 1 cut(s) 415
BmrFI CCNGG 2 cut(s) 14, 466
BmrI ACTGGG 1 cut(s) 42
BmsI GCATC 1 cut(s) 255
BmuI ACTGGG 1 cut(s) 42
BpiI GAAGAC 1 cut(s) 63
Bpu14I TTCGAA 1 cut(s) 306
BpuMI CCSGG 1 cut(s) 466
BsaBI GATNNNNATC 1 cut(s) 384
BsaJI CCNNGG 1 cut(s) 484
Bse118I RCCGGY 1 cut(s) 176
Bse1I ACTGG 4 cut(s) 48, 85, 372, 418
Bse8I GATNNNNATC 1 cut(s) 384
BseBI CCWGG 1 cut(s) 14
BseDI CCNNGG 1 cut(s) 484
BseGI GGATG 2 cut(s) 182, 360
BseJI GATNNNNATC 1 cut(s) 384
BseNI ACTGG 4 cut(s) 48, 85, 372, 418
BseRI GAGGAG 1 cut(s) 382
Bsh1236I CGCG 1 cut(s) 190
BshFI GGCC 3 cut(s) 417, 469, 483
BsiHKAI GWGCWC 1 cut(s) 112
BsiSI CCGG 2 cut(s) 177, 466
BsnI GGCC 3 cut(s) 417, 469, 483
Bsp119I TTCGAA 1 cut(s) 306
Bsp1286I GDGCHC 1 cut(s) 112
Bsp143I GATC 2 cut(s) 379, 385
BspACI CCGC 2 cut(s) 36, 188
BspANI GGCC 3 cut(s) 417, 469, 483
BspFNI CGCG 1 cut(s) 190
BspT104I TTCGAA 1 cut(s) 306
BsrFI RCCGGY 1 cut(s) 176
BsrI ACTGG 4 cut(s) 48, 85, 372, 418
BssAI RCCGGY 1 cut(s) 176
BssECI CCNNGG 1 cut(s) 484
BssMI GATC 2 cut(s) 379, 385
BssSI CACGAG 3 cut(s) 111, 121, 257
BssT1I CCWWGG 1 cut(s) 484
Bst2BI CACGAG 3 cut(s) 111, 121, 257
Bst2UI CCWGG 1 cut(s) 14
Bst4CI ACNGT 1 cut(s) 62
Bst6I CTCTTC 1 cut(s) 12
BstAPI GCANNNNNTGC 1 cut(s) 33
BstBI TTCGAA 1 cut(s) 306
BstC8I GCNNGC 2 cut(s) 108, 270
BstF5I GGATG 2 cut(s) 182, 360
BstFNI CGCG 1 cut(s) 190
BstKTI GATC 2 cut(s) 382, 388
BstMBI GATC 2 cut(s) 379, 385
BstMWI GCNNNNNNNGC 2 cut(s) 33, 506
BstNI CCWGG 1 cut(s) 14
BstNSI RCATGY 1 cut(s) 272
BstSCI CCNGG 2 cut(s) 12, 464
BstUI CGCG 1 cut(s) 190
BstV2I GAAGAC 1 cut(s) 63
BsuI GTATCC 1 cut(s) 482
BsuRI GGCC 3 cut(s) 417, 469, 483
BtsCI GGATG 2 cut(s) 182, 360
BtsIMutI CAGTG 1 cut(s) 379
Cac8I GCNNGC 2 cut(s) 108, 270
Cfr10I RCCGGY 1 cut(s) 176
Cfr13I GGNCC 1 cut(s) 415
Csp6I GTAC 2 cut(s) 51, 224
CviAII CATG 2 cut(s) 269, 273
CviJI RGCY 6 cut(s) 180, 417, 469, 483, 500, 525
CviKI_1 RGCY 6 cut(s) 180, 417, 469, 483, 500, 525
CviQI GTAC 2 cut(s) 51, 224
DpnI GATC 2 cut(s) 381, 387
DpnII GATC 2 cut(s) 379, 385
DrdI GACNNNNNNGTC 1 cut(s) 236
DseDI GACNNNNNNGTC 1 cut(s) 236
EaeI YGGCCR 2 cut(s) 467, 481
Eam1104I CTCTTC 1 cut(s) 12
EarI CTCTTC 1 cut(s) 12
Eco130I CCWWGG 1 cut(s) 484
Eco32I GATATC 1 cut(s) 157
EcoRII CCWGG 1 cut(s) 12
EcoRV GATATC 1 cut(s) 157
EcoT14I CCWWGG 1 cut(s) 484
EcoT22I ATGCAT 2 cut(s) 270, 274
ErhI CCWWGG 1 cut(s) 484
FaeI CATG 2 cut(s) 272, 276
FaiI YATR 7 cut(s) 89, 270, 274, 397, 408, 480, 546
FalI AAGNNNNNCTT 2 cut(s) 509, 541
FatI CATG 2 cut(s) 268, 272
FauI CCCGC 1 cut(s) 195
FbaI TGATCA 1 cut(s) 385
FblI GTMKAC 1 cut(s) 9
FokI GGATG 2 cut(s) 169, 367
FspBI CTAG 3 cut(s) 99, 209, 504
HaeIII GGCC 3 cut(s) 417, 469, 483
HapII CCGG 2 cut(s) 177, 466
Hin1II CATG 2 cut(s) 272, 276
HincII GTYRAC 1 cut(s) 10
HindII GTYRAC 1 cut(s) 10
HinfI GANTC 2 cut(s) 452, 535
HpaII CCGG 2 cut(s) 177, 466
Hpy166II GTNNAC 1 cut(s) 10
Hpy188I TCNGA 1 cut(s) 221
Hpy188III TCNNGA 3 cut(s) 121, 287, 383
Hpy8I GTNNAC 1 cut(s) 10
HpyAV CCTTC 2 cut(s) 293, 317
HpyCH4III ACNGT 1 cut(s) 62
HpyCH4IV ACGT 1 cut(s) 31
HpyCH4V TGCA 4 cut(s) 27, 268, 272, 280
HpyF10VI GCNNNNNNNGC 2 cut(s) 33, 506
HpySE526I ACGT 1 cut(s) 31
Hsp92II CATG 2 cut(s) 272, 276
Ksp22I TGATCA 1 cut(s) 385
Kzo9I GATC 2 cut(s) 379, 385
LweI GCATC 1 cut(s) 255
MaeI CTAG 3 cut(s) 99, 209, 504
MaeII ACGT 1 cut(s) 31
MaeIII GTNAC 1 cut(s) 191
MalI GATC 2 cut(s) 381, 387
MboI GATC 2 cut(s) 379, 385
MboII GAAGA 5 cut(s) 29, 68, 164, 320, 332
MhlI GDGCHC 1 cut(s) 112
MlsI TGGCCA 1 cut(s) 483
MluCI AATT 6 cut(s) 144, 198, 204, 302, 431, 440
MluNI TGGCCA 1 cut(s) 483
MlyI GAGTC 1 cut(s) 529
MnlI CCTC 3 cut(s) 403, 490, 579
Mox20I TGGCCA 1 cut(s) 483
Mph1103I ATGCAT 2 cut(s) 270, 274
MroXI GAANNNNTTC 1 cut(s) 302
MscI TGGCCA 1 cut(s) 483
MseI TTAA 1 cut(s) 423
Msp20I TGGCCA 1 cut(s) 483
MspI CCGG 2 cut(s) 177, 466
MspR9I CCNGG 2 cut(s) 14, 466
MvaI CCWGG 1 cut(s) 14
MvnI CGCG 1 cut(s) 190
MwoI GCNNNNNNNGC 2 cut(s) 33, 506
NciI CCSGG 1 cut(s) 466
NdeII GATC 2 cut(s) 379, 385
NlaIII CATG 2 cut(s) 272, 276
NsiI ATGCAT 2 cut(s) 270, 274
NspI RCATGY 1 cut(s) 272
NspV TTCGAA 1 cut(s) 306
PaeI GCATGC 1 cut(s) 272
PcsI WCGNNNNNNNCGW 1 cut(s) 109
PdmI GAANNNNTTC 1 cut(s) 302
PfeI GAWTC 1 cut(s) 452
PleI GAGTC 1 cut(s) 529
PpsI GAGTC 1 cut(s) 529
Psp1406I AACGTT 1 cut(s) 31
Psp6I CCWGG 1 cut(s) 12
PspGI CCWGG 1 cut(s) 12
PspPI GGNCC 1 cut(s) 415
RsaI GTAC 2 cut(s) 52, 225
RsaNI GTAC 2 cut(s) 51, 224
SalI GTCGAC 1 cut(s) 8
SaqAI TTAA 1 cut(s) 423
Sau3AI GATC 2 cut(s) 379, 385
Sau96I GGNCC 1 cut(s) 415
SchI GAGTC 1 cut(s) 529
ScrFI CCNGG 2 cut(s) 14, 466
SduI GDGCHC 1 cut(s) 112
SetI ASST 6 cut(s) 34, 234, 285, 318, 365, 407
SfaNI GCATC 1 cut(s) 255
SfuI TTCGAA 1 cut(s) 306
SphI GCATGC 1 cut(s) 272
Sse9I AATT 6 cut(s) 144, 198, 204, 302, 431, 440
SsiI CCGC 2 cut(s) 36, 188
SspMI CTAG 3 cut(s) 99, 209, 504
StyD4I CCNGG 2 cut(s) 12, 464
StyI CCWWGG 1 cut(s) 484
TaaI ACNGT 1 cut(s) 62
TaiI ACGT 1 cut(s) 34
TaqI TCGA 2 cut(s) 9, 306
TasI AATT 6 cut(s) 144, 198, 204, 302, 431, 440
TatI WGTACW 1 cut(s) 50
TfiI GAWTC 1 cut(s) 452
Tru1I TTAA 1 cut(s) 423
Tru9I TTAA 1 cut(s) 423
TscAI CASTG 1 cut(s) 379
TspRI CASTG 1 cut(s) 379
XapI RAATTY 1 cut(s) 302
XceI RCATGY 1 cut(s) 272
XmiI GTMKAC 1 cut(s) 9
XmnI GAANNNNTTC 1 cut(s) 302
XspI CTAG 3 cut(s) 99, 209, 504
Zsp2I ATGCAT 2 cut(s) 270, 274
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.