RchiOBHm_Chr4g0407761

Vacuolar protein sorting-associated protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
4
OG0000415
Physical Location & Seq
Forward (+)
30073832 .. 30076605
2774 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ37898

Sequence Viewer

Length: 1209 bp
ATGCTCCTGAAGAAAAGCTGGTTTTCATTTAGATGGCGTACGCCTGATGAATCTGATGATGATACTTCTGAGGGATCACGGTCACCAGAAGAAAGACTGACTGAAGAGGAGTGGCAAGCAATTAATAAGTTACTAAGTTACCAGCCAGATGAAACATTGGCATCTCACTCAGGAAAGGACATGCCGAATATGATCCTGTTTATGGTAACTGTGTCTGTTGATCAGGCTGCTGCTAGGATCGTTGATATAAATCACACTGAGATTTTGTGTTGTAAATTTGAGCAACTTCAAGTGTCTACGAAGTCTAAACATCAAAGTACATACTGTGATGTGTCATGCAGATTCTATGGTCTGTATGCACCGGAAGGCTCGCTTGCTCAGAGTGTATCTAGTGAGCAGAAGGCGAATGCACTGGCTGCTAGCTTTGTATACCATCCAGTTGGAGAGAATGTTGATCAGAGGCTTTCAGCTACCATTTCACCTTGTCATGTCACGGTTTTGATGGAAAGCCGCGATCGCTTTTTGGATTTTGTGAAAAGGAGTAATGCAGTTAGTCCTACTGTTGCATTGGAAACGGCAACTGCTTTGCAGATGCCAATTGAGAAAGTGACTCGTAGAGCACAAGAGCAATTTCAAATGGTATTAGAAGAACAAAGCAGATTTGCCCTTGATATTGATTTGGATGCTCCAAAAGTAAGAATTCCTATCAGAACCAAGGGCTCTTCCAAATGTGATAGCCATTTTCTATTGGATCTTGGTCATTTCACCCTGCAAACGAAGGATACTCAGCATGATAAACAGAAGAAGAACATGTATTCTCGTTTTTTTATAACTGGAAGAGATATTGCTGCATTTTTTACGGATTGTGGTTCCGATTGCCAAATTCGCACTTTGGAATCACCTGATTCTGATAACCACGCACCGGTTACCCTGGAAACTGTTGACAATTTCTATTCTCTCATAGATAGGTGTGGAATGGCTCCACTTGTTGATCAGATCAGAGTACCTCACCCAAGTTACCTGTCAATGCGTATTTCAATTCAAGTGCCAAACCTCCGAGTGCACTTTTCACCATCGAGATTTCAGAGACTCATGAAATTATTAAACATATTTTATGGTACTCTGGAAACTGTTGGTCAGCCAGCTGTTGATAATTTTCAAGCAGAACGTGCCCCATGGAGTCCTGCAGATCTTTTACACGGATGCTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
C2 Chorein_N Pex24p SHR-BD VPS13 VPS13_C VPS13_mid_rpt Vps62

Protein Analysis

402

Amino Acids

45.59

Weight (kDa)

5.77

Isoelectric Point (pI)

50.99

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
VPS13_N PF12624 130 - 268 1.9e-07 VPS13-like family N-terminal region
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000415)

Species Orthologous Gene IDs
fragaria_vesca FvH4_2g17790 FvH4_2g20000 FvH4_5g06730 FvH4_5g06800 FvH4_5g06820 FvH4_6g04440 FvH4_6g04540 FvH4_6g34210
malus_domestica MD10G1023300.v1.1 MD14G1198500.v1.1
prunus_persica Prupe.2G100600_v2.0.a1 Prupe.8G205400_v2.0.a1 Prupe.8G205500_v2.0.a1
rosa_chinensis RchiOBHm_Chr1g0321451 RchiOBHm_Chr2g0160021 RchiOBHm_Chr4g0407711 RchiOBHm_Chr4g0407721 RchiOBHm_Chr4g0407751 RchiOBHm_Chr4g0407761 RchiOBHm_Chr6g0282811 RchiOBHm_Chr6g0285651 RchiOBHm_Chr6g0285661 RchiOBHm_Chr6g0285691 RchiOBHm_Chr6g0285701 RchiOBHm_Chr7g0191701
rosa_laevigata RLG00000001989 RLG00000003825 RLG00000004431 RLG00000012616 RLG00000012797 RLG00000012866 RLG00000015356 RLG00000021192 RLG00000025443
rosa_multiflora Rmu_sc0000600.1_g000050 Rmu_sc0000600.1_g000051 Rmu_sc0000600.1_g000053 Rmu_sc0000600.1_g000054 Rmu_sc0001123.1_g000019 Rmu_sc0001123.1_g000023 Rmu_sc0001521.1_g000015 Rmu_sc0002023.1_g000017
rosa_roxburghii Rroxscaffold_157G00438540 Rroxscaffold_2G00090090 Rroxscaffold_3G00263620 Rroxscaffold_7G00182850 Rroxscaffold_7G00185190 Rroxscaffold_7G00186010 Rroxscaffold_7G00215810
rosa_rugosa Rorug02G0479800 Rorug03G0030400 Rorug03G0077500 Rorug04G0221600 Rorug06G0006300 Rorug06G0157700 Rorug06G0176400 Rorug06G0511100 Rorug07G0052100 Rorug07G0220500
rosa_samantha Rh2AG546400 Rh2BG559800 Rh2CG529800 Rh2DG568400 Rh2DG568500 Rh3BG143100 Rh3DG144300 Rh4AG146200 Rh4AG146300 Rh4BG153900 Rh4CG152800 Rh6AG024300 Rh6AG262900 Rh6AG270400 Rh6AG288000 Rh6BG265800 Rh6BG272000 Rh6BG289500 Rh6CG016700 Rh6CG265100 Rh6CG272600 Rh6CG272700 Rh6CG290500 Rh6CG290700 Rh6DG017800 Rh6DG257600 Rh6DG265800 Rh6DG283200 Rh7AG119900 Rh7AG120400 Rh7BG122400 Rh7BG178300 Rh7CG125000 Rh7CG125100 Rh7DG123200 Rh7DG123500 Rh7DG123600
rosa_wichuraiana Rw4G011890 Rw4G011910 Rw4G011920

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
AccI GTMKAC 2 cut(s) 296, 429
AccII CGCG 1 cut(s) 513
AciI CCGC 1 cut(s) 511
AclWI GGATC 4 cut(s) 82, 187, 245, 759
AcsI RAATTY 3 cut(s) 275, 699, 882
AcuI CTGAAG 2 cut(s) 29, 123
AfaI GTAC 4 cut(s) 40, 319, 1005, 1120
AfiI CCNNNNNNNGG 2 cut(s) 202, 922
AflIII ACRYGT 1 cut(s) 810
AgeI ACCGGT 1 cut(s) 922
AgsI TTSAA 5 cut(s) 290, 635, 1038, 1043, 1160
AjnI CCWGG 1 cut(s) 930
AluBI AGCT 4 cut(s) 18, 423, 470, 1145
AluI AGCT 4 cut(s) 18, 423, 470, 1145
Alw21I GWGCWC 2 cut(s) 622, 1065
Alw26I GTCTC 1 cut(s) 1081
Alw44I GTGCAC 1 cut(s) 1061
AlwI GGATC 4 cut(s) 82, 187, 245, 759
ApaLI GTGCAC 1 cut(s) 1061
ApeKI GCWGC 4 cut(s) 227, 230, 416, 848
ApoI RAATTY 3 cut(s) 275, 699, 882
AseI ATTAAT 1 cut(s) 123
AsiGI ACCGGT 1 cut(s) 922
AsiSI GCGATCGC 1 cut(s) 517
AsuHPI GGTGA 6 cut(s) 75, 471, 757, 891, 1001, 1062
AsuNHI GCTAGC 1 cut(s) 419
BaeGI GKGCMC 2 cut(s) 1065, 1174
BanII GRGCYC 1 cut(s) 722
Bbv12I GWGCWC 2 cut(s) 622, 1065
BbvI GCAGC 4 cut(s) 214, 217, 403, 835
BccI CCATC 4 cut(s) 27, 441, 496, 1081
BceAI ACGGC 1 cut(s) 591
BciT130I CCWGG 1 cut(s) 932
BciVI GTATCC 1 cut(s) 775
BclI TGATCA 3 cut(s) 220, 454, 991
BcoDI GTCTC 1 cut(s) 1081
BfaI CTAG 4 cut(s) 234, 390, 420, 1207
BfmI CTRYAG 1 cut(s) 1185
BfuI GTATCC 1 cut(s) 775
BglII AGATCT 1 cut(s) 1189
BisI GCNGC 5 cut(s) 228, 231, 417, 511, 849
BlsI GCNGC 5 cut(s) 229, 232, 418, 512, 850
Bme1390I CCNGG 1 cut(s) 932
BmiI GGNNCC 2 cut(s) 871, 981
BmrFI CCNGG 1 cut(s) 932
BmsI GCATC 4 cut(s) 170, 582, 673, 1193
BmtI GCTAGC 1 cut(s) 423
BsaBI GATNNNNATC 1 cut(s) 249
BsaJI CCNNGG 3 cut(s) 714, 930, 1175
BsaWI WCCGGW 2 cut(s) 361, 922
BsaXI ACNNNNNCTCC 2 cut(s) 435, 465
Bsc4I CCNNNNNNNGG 2 cut(s) 202, 922
Bse118I RCCGGY 1 cut(s) 922
Bse1I ACTGG 3 cut(s) 417, 437, 838
Bse8I GATNNNNATC 1 cut(s) 249
BseBI CCWGG 1 cut(s) 932
BseDI CCNNGG 3 cut(s) 714, 930, 1175
BseGI GGATG 3 cut(s) 433, 688, 1208
BseJI GATNNNNATC 1 cut(s) 249
BseLI CCNNNNNNNGG 2 cut(s) 202, 922
BseMII CTCAG 5 cut(s) 60, 183, 249, 392, 800
BseNI ACTGG 3 cut(s) 417, 437, 838
BseRI GAGGAG 1 cut(s) 122
BseSI GKGCMC 2 cut(s) 1065, 1174
BseXI GCAGC 4 cut(s) 214, 217, 403, 835
Bsh1236I CGCG 1 cut(s) 513
Bsh1285I CGRYCG 1 cut(s) 517
BshTI ACCGGT 1 cut(s) 922
BsiEI CGRYCG 1 cut(s) 517
BsiHKAI GWGCWC 2 cut(s) 622, 1065
BsiSI CCGG 2 cut(s) 362, 923
BsiWI CGTACG 1 cut(s) 38
BslI CCNNNNNNNGG 2 cut(s) 202, 922
BsmAI GTCTC 1 cut(s) 1081
BsmI GAATGC 1 cut(s) 412
Bsp1286I GDGCHC 4 cut(s) 622, 722, 1065, 1174
Bsp19I CCATGG 1 cut(s) 1175
BspACI CCGC 1 cut(s) 511
BspCNI CTCAG 5 cut(s) 61, 182, 250, 391, 799
BspFNI CGCG 1 cut(s) 513
BspHI TCATGA 1 cut(s) 1092
BspLI GGNNCC 2 cut(s) 871, 981
BspMAI CTGCAG 1 cut(s) 1189
BspOI GCTAGC 1 cut(s) 423
BspPI GGATC 4 cut(s) 82, 187, 245, 759
BspQI GCTCTTC 1 cut(s) 727
BsrFI RCCGGY 1 cut(s) 922
BsrI ACTGG 3 cut(s) 417, 437, 838
BssAI RCCGGY 1 cut(s) 922
BssECI CCNNGG 3 cut(s) 714, 930, 1175
BssNAI GTATAC 1 cut(s) 430
BssT1I CCWWGG 2 cut(s) 714, 1175
Bst1107I GTATAC 1 cut(s) 430
Bst2UI CCWGG 1 cut(s) 932
Bst4CI ACNGT 7 cut(s) 81, 211, 326, 496, 562, 940, 1132
Bst6I CTCTTC 3 cut(s) 99, 727, 832
BstAPI GCANNNNNTGC 2 cut(s) 416, 1169
BstC8I GCNNGC 5 cut(s) 117, 371, 375, 421, 1143
BstDEI CTNAG 6 cut(s) 69, 134, 169, 258, 378, 786
BstDSI CCRYGG 1 cut(s) 1175
BstEII GGTNACC 2 cut(s) 81, 925
BstF5I GGATG 3 cut(s) 433, 688, 1208
BstFNI CGCG 1 cut(s) 513
BstMAI GTCTC 1 cut(s) 1081
BstMCI CGRYCG 1 cut(s) 517
BstMWI GCNNNNNNNGC 4 cut(s) 416, 516, 885, 1169
BstNI CCWGG 1 cut(s) 932
BstNSI RCATGY 2 cut(s) 184, 814
BstPI GGTNACC 2 cut(s) 81, 925
BstSCI CCNGG 1 cut(s) 930
BstSFI CTRYAG 1 cut(s) 1185
BstSLI GKGCMC 2 cut(s) 1065, 1174
BstUI CGCG 1 cut(s) 513
BstV1I GCAGC 4 cut(s) 214, 217, 403, 835
BstX2I RGATCY 2 cut(s) 751, 1189
BstXI CCANNNNNNTGG 1 cut(s) 440
BstYI RGATCY 2 cut(s) 751, 1189
BstZ17I GTATAC 1 cut(s) 430
BsuI GTATCC 1 cut(s) 775
BtgI CCRYGG 1 cut(s) 1175
BtsCI GGATG 3 cut(s) 433, 688, 1208
BtsIMutI CAGTG 2 cut(s) 255, 410
Cac8I GCNNGC 5 cut(s) 117, 371, 375, 421, 1143
CciI TCATGA 1 cut(s) 1092
Cfr10I RCCGGY 1 cut(s) 922
Csp6I GTAC 4 cut(s) 39, 318, 1004, 1119
CspAI ACCGGT 1 cut(s) 922
CviAII CATG 7 cut(s) 181, 336, 488, 791, 811, 1093, 1176
CviQI GTAC 4 cut(s) 39, 318, 1004, 1119
DdeI CTNAG 6 cut(s) 69, 134, 169, 258, 378, 786
Eam1104I CTCTTC 3 cut(s) 99, 727, 832
EarI CTCTTC 3 cut(s) 99, 727, 832
Eco130I CCWWGG 2 cut(s) 714, 1175
Eco24I GRGCYC 1 cut(s) 722
Eco57I CTGAAG 2 cut(s) 29, 123
Eco91I GGTNACC 2 cut(s) 81, 925
EcoO65I GGTNACC 2 cut(s) 81, 925
EcoRI GAATTC 1 cut(s) 699
EcoRII CCWGG 1 cut(s) 930
EcoT14I CCWWGG 2 cut(s) 714, 1175
EcoT38I GRGCYC 1 cut(s) 722
ErhI CCWWGG 2 cut(s) 714, 1175
FaeI CATG 7 cut(s) 184, 339, 491, 794, 814, 1096, 1179
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FatI CATG 7 cut(s) 180, 335, 487, 790, 810, 1092, 1175
FbaI TGATCA 3 cut(s) 220, 454, 991
FblI GTMKAC 2 cut(s) 296, 429
Fnu4HI GCNGC 5 cut(s) 228, 231, 417, 511, 849
FokI GGATG 2 cut(s) 420, 695
FriOI GRGCYC 1 cut(s) 722
Fsp4HI GCNGC 5 cut(s) 228, 231, 417, 511, 849
FspBI CTAG 4 cut(s) 234, 390, 420, 1207
GluI GCNGC 5 cut(s) 228, 231, 417, 511, 849
HapII CCGG 2 cut(s) 362, 923
Hin1II CATG 7 cut(s) 184, 339, 491, 794, 814, 1096, 1179
HincII GTYRAC 1 cut(s) 943
HindII GTYRAC 1 cut(s) 943
HinfI GANTC 7 cut(s) 50, 342, 610, 896, 905, 1089, 1180
HpaII CCGG 2 cut(s) 362, 923
HphI GGTGA 6 cut(s) 75, 471, 757, 891, 1001, 1062
Hpy166II GTNNAC 4 cut(s) 297, 430, 943, 1063
Hpy188III TCNNGA 5 cut(s) 7, 171, 1077, 1093, 1124
Hpy8I GTNNAC 4 cut(s) 297, 430, 943, 1063
HpyAV CCTTC 3 cut(s) 359, 394, 772
HpyCH4III ACNGT 7 cut(s) 81, 211, 326, 496, 562, 940, 1132
HpyCH4IV ACGT 1 cut(s) 1168
HpyF10VI GCNNNNNNNGC 4 cut(s) 416, 516, 885, 1169
HpyF3I CTNAG 6 cut(s) 69, 134, 169, 258, 378, 786
HpySE526I ACGT 1 cut(s) 1168
Hsp92II CATG 7 cut(s) 184, 339, 491, 794, 814, 1096, 1179
Ksp22I TGATCA 3 cut(s) 220, 454, 991
LguI GCTCTTC 1 cut(s) 727
LmnI GCTCC 3 cut(s) 9, 691, 985
Lsp1109I GCAGC 4 cut(s) 214, 217, 403, 835
LweI GCATC 4 cut(s) 170, 582, 673, 1193
MaeI CTAG 4 cut(s) 234, 390, 420, 1207
MaeII ACGT 1 cut(s) 1168
MaeIII GTNAC 8 cut(s) 81, 129, 137, 205, 490, 607, 925, 1016
MboII GAAGA 8 cut(s) 22, 101, 116, 659, 714, 814, 817, 849
MfeI CAATTG 1 cut(s) 597
MflI RGATCY 2 cut(s) 751, 1189
MhlI GDGCHC 4 cut(s) 622, 722, 1065, 1174
MlyI GAGTC 3 cut(s) 604, 1083, 1189
MmeI TCCRAC 1 cut(s) 421
MnlI CCTC 5 cut(s) 64, 100, 453, 1017, 1064
MseI TTAA 2 cut(s) 123, 1103
MslI CAYNNNNRTG 1 cut(s) 31
MspA1I CMGCKG 1 cut(s) 1145
MspI CCGG 2 cut(s) 362, 923
MspR9I CCNGG 1 cut(s) 932
MunI CAATTG 1 cut(s) 597
Mva1269I GAATGC 1 cut(s) 412
MvaI CCWGG 1 cut(s) 932
MvnI CGCG 1 cut(s) 513
MwoI GCNNNNNNNGC 4 cut(s) 416, 516, 885, 1169
NcoI CCATGG 1 cut(s) 1175
NheI GCTAGC 1 cut(s) 419
NlaIII CATG 7 cut(s) 184, 339, 491, 794, 814, 1096, 1179
NlaIV GGNNCC 2 cut(s) 871, 981
NmuCI GTSAC 3 cut(s) 81, 490, 607
NspI RCATGY 2 cut(s) 184, 814
PagI TCATGA 1 cut(s) 1092
PciI ACATGT 1 cut(s) 810
PciSI GCTCTTC 1 cut(s) 727
PctI GAATGC 1 cut(s) 412
PfeI GAWTC 4 cut(s) 50, 342, 896, 905
Pfl23II CGTACG 1 cut(s) 38
PinAI ACCGGT 1 cut(s) 922
PkrI GCNGC 5 cut(s) 229, 232, 418, 512, 850
Ple19I CGATCG 1 cut(s) 517
PleI GAGTC 3 cut(s) 604, 1083, 1188
PpsI GAGTC 3 cut(s) 604, 1083, 1188
PscI ACATGT 1 cut(s) 810
PshBI ATTAAT 1 cut(s) 123
PsiI TTATAA 1 cut(s) 830
Psp6I CCWGG 1 cut(s) 930
PspEI GGTNACC 2 cut(s) 81, 925
PspGI CCWGG 1 cut(s) 930
PspLI CGTACG 1 cut(s) 38
PspN4I GGNNCC 2 cut(s) 871, 981
PstI CTGCAG 1 cut(s) 1189
PsuI RGATCY 2 cut(s) 751, 1189
PvuI CGATCG 1 cut(s) 517
PvuII CAGCTG 1 cut(s) 1145
RgaI GCGATCGC 1 cut(s) 517
RsaI GTAC 4 cut(s) 40, 319, 1005, 1120
RsaNI GTAC 4 cut(s) 39, 318, 1004, 1119
RseI CAYNNNNRTG 1 cut(s) 31
SapI GCTCTTC 1 cut(s) 727
SaqAI TTAA 2 cut(s) 123, 1103
SatI GCNGC 5 cut(s) 228, 231, 417, 511, 849
SchI GAGTC 3 cut(s) 604, 1083, 1189
ScrFI CCNGG 1 cut(s) 932
SduI GDGCHC 4 cut(s) 622, 722, 1065, 1174
SfaAI GCGATCGC 1 cut(s) 517
SfaNI GCATC 4 cut(s) 170, 582, 673, 1193
SfcI CTRYAG 1 cut(s) 1185
SgfI GCGATCGC 1 cut(s) 517
SmiMI CAYNNNNRTG 1 cut(s) 31
SsiI CCGC 1 cut(s) 511
SspMI CTAG 4 cut(s) 234, 390, 420, 1207
StyD4I CCNGG 1 cut(s) 930
StyI CCWWGG 2 cut(s) 714, 1175
TaaI ACNGT 7 cut(s) 81, 211, 326, 496, 562, 940, 1132
TaiI ACGT 1 cut(s) 1171
TaqI TCGA 1 cut(s) 1076
TatI WGTACW 1 cut(s) 317
TauI GCSGC 1 cut(s) 513
TfiI GAWTC 4 cut(s) 50, 342, 896, 905
Tru1I TTAA 2 cut(s) 123, 1103
Tru9I TTAA 2 cut(s) 123, 1103
TscAI CASTG 2 cut(s) 262, 417
TseFI GTSAC 3 cut(s) 81, 490, 607
TseI GCWGC 4 cut(s) 227, 230, 416, 848
Tsp45I GTSAC 3 cut(s) 81, 490, 607
TspDTI ATGAA 4 cut(s) 15, 63, 165, 1109
TspGWI ACGGA 1 cut(s) 875
TspRI CASTG 2 cut(s) 262, 417
VneI GTGCAC 1 cut(s) 1061
VspI ATTAAT 1 cut(s) 123
XapI RAATTY 3 cut(s) 275, 699, 882
XceI RCATGY 2 cut(s) 184, 814
XmiI GTMKAC 2 cut(s) 296, 429
XspI CTAG 4 cut(s) 234, 390, 420, 1207
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.