FvH4_3g43500

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000117
Physical Location & Seq
Forward (+)
36234095 .. 36237694
3600 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43500.t1

Sequence Viewer

Length: 1548 bp
ATGAAAATCCAGGGGTCTTTGCTTCCAGCCATAGTGCTTCTGCTTCTTGGCTTTGGATTGAGAAATGGTAAAGCCAGTGATACACCCCCTGCTCATTATGACACCGCTTTCCTCAACAGGACTAGTTTCCCAGCAGGTTTCATATTTGGGACAGCTTCATCATCTTACCAGTATGAAGGTGCTGCTCAAGATGGTGGCAGGGGACCAAGCATATGGGATACCTATACCCACAGATATCCAGACAAGATTAAGGACGGTAGCAATGGAGATGTAGCTAATGATGAATATCACCGCTATAAGGAAGATGTAGGGATTATGAAGAACATGGGGTTGGATGCATATCGGTTCTCTATCTCATGGTCCAGATTGTTACCAAATGGAAAGCTAAGTGGGGGAGTGAACAAGAAAGGAGTCAAATACTACAACAATCTCATCAATGAACTCCTACGCAATGGCATAAAACCATTTGTGACCCTCTTCCACTGGGATCTTCCCCAAACTTTAGAAGATGAGTATGGCGGTTTTTTAAGCCCTCACATTGTCAATCATTTTAGGGACTACGCAGAGCTTTGTTACAAGGAATTTGGTGACAGGGTAAAGCACTGGATCACGTTTAATGAGCCATATAGTTATAGTGCTGGTGGTTATGCAGCGGCAATTTTGGCACCAGGACGCTGTTCTGATTGGCAACAGCTAAACTGCACCGGTGGGGATTCCGGTACTGAACCATATTTGGTAGCACACCACCAGCTCCTTGCTCATGCAGCTGCAGTAAAATTATACAGGCAAAAATATCAGTCATCTCAAAAGGGATTGATAGGGATTACTCTAGTTTCAAATTGGTATGTGCCGGTTTCGGAGGCAAAGCACCATAAAAATGCTGCACTAAGAGCTCTAGATTACATGCTTGGATGGTTTATGGATCCCTTGACAAATGGAGATTATCCACACAGCATGAGGTCTCTAGTTGGAAACCGATTACCAAAATTCACAAAGGAGCAGTCCAAATTGTTAATTGGATCATTTGATTTTCTGGGATTGAATTATTACACAGCTAATTATGCAGCCTATGCACCTCAACATAATTCTGTCAATGCAAGCTACTTAACAGATGCTCGTGCTTCTCTTTCATCTGTGCTTAATGGAATTCCCATTGGACCAAAGGCTGCTTCAGACTGGCTATACGTTTATCCAAGAGGAATTCGAGATCTTTTGCTCTACACAAAGACAAAATATAATGATCCACTCATATACATTACTGAGAATGGTATTGATGAGTACAATGATCCGCAATTATCACTTGAAGAAGCCCTTAACGACACGCAGAGAATCGACTACTACTATCGCCACCTGTATTACCTTCAAAGAGCAATCAAGGATGGTGTGAATGTGAAAGGATACTTTGCATGGTCACTGCTAGACAATTTTGAATGGAATTCTGGTTATACAGTCCGATTTGGAATCAACTATGTAGATTACAAAAATGGGCAGACTAGGCACCCAAAACTTTCTGCACACTGGTTCAAAGGTTTTCTGAAGAAAACGTAA

Protein Analysis

516

Amino Acids

58.46

Weight (kDa)

8.76

Isoelectric Point (pI)

25.69

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 41 - 513 5.5e-159 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 125
AccB1I GGYRCC 2 cut(s) 664, 1497
AciI CCGC 5 cut(s) 105, 292, 519, 653, 1289
AclWI GGATC 7 cut(s) 495, 614, 917, 930, 1027, 1235, 1280
AcsI RAATTY 5 cut(s) 581, 986, 1146, 1200, 1435
AcuI CTGAAG 1 cut(s) 1155
AfaI GTAC 2 cut(s) 721, 1280
AfiI CCNNNNNNNGG 1 cut(s) 298
AgeI ACCGGT 1 cut(s) 704
AgsI TTSAA 6 cut(s) 837, 1042, 1304, 1364, 1430, 1525
AhlI ACTAGT 1 cut(s) 122
AjnI CCWGG 2 cut(s) 9, 667
AjuI GAANNNNNNNTTGG 4 cut(s) 314, 346, 1153, 1185
Alw21I GWGCWC 1 cut(s) 895
Alw26I GTCTC 1 cut(s) 966
AlwI GGATC 7 cut(s) 495, 614, 917, 930, 1027, 1235, 1280
ApeKI GCWGC 7 cut(s) 182, 650, 764, 767, 881, 1064, 1166
ApoI RAATTY 5 cut(s) 581, 986, 1146, 1200, 1435
ArsI GACNNNNNNTTYG 2 cut(s) 986, 1018
AsiGI ACCGGT 1 cut(s) 704
AspS9I GGNCC 3 cut(s) 203, 360, 1157
AsuHPI GGTGA 2 cut(s) 281, 599
AvaII GGWCC 3 cut(s) 203, 360, 1157
BamHI GGATCC 1 cut(s) 922
BanI GGYRCC 2 cut(s) 664, 1497
BanII GRGCYC 1 cut(s) 895
BauI CACGAG 1 cut(s) 1116
Bbv12I GWGCWC 1 cut(s) 895
BbvI GCAGC 7 cut(s) 169, 662, 754, 776, 868, 1076, 1153
BccI CCATC 3 cut(s) 185, 906, 1373
BciT130I CCWGG 2 cut(s) 11, 669
BciVI GTATCC 2 cut(s) 211, 1391
BcoDI GTCTC 1 cut(s) 966
BcuI ACTAGT 1 cut(s) 122
BfaI CTAG 6 cut(s) 123, 830, 896, 965, 1418, 1494
BfmI CTRYAG 1 cut(s) 768
BfuAI ACCTGC 1 cut(s) 125
BfuI GTATCC 2 cut(s) 211, 1391
BglII AGATCT 1 cut(s) 1207
BisI GCNGC 8 cut(s) 183, 651, 654, 765, 768, 882, 1065, 1167
BlsI GCNGC 8 cut(s) 184, 652, 655, 766, 769, 883, 1066, 1168
Bme1390I CCNGG 2 cut(s) 11, 669
Bme18I GGWCC 3 cut(s) 203, 360, 1157
BmgT120I GGNCC 3 cut(s) 203, 360, 1157
BmiI GGNNCC 4 cut(s) 204, 666, 924, 1499
BmrFI CCNGG 2 cut(s) 11, 669
BmrI ACTGGG 1 cut(s) 493
BmsI GCATC 2 cut(s) 325, 1102
BmuI ACTGGG 1 cut(s) 493
BpuEI CTTGAG 1 cut(s) 171
BsaBI GATNNNNATC 2 cut(s) 285, 339
BsaI GGTCTC 1 cut(s) 966
BsaJI CCNNGG 1 cut(s) 10
BsaWI WCCGGW 2 cut(s) 704, 716
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 2 cut(s) 704, 850
Bse1I ACTGG 6 cut(s) 75, 169, 488, 608, 1181, 1523
Bse3DI GCAATG 2 cut(s) 268, 457
Bse8I GATNNNNATC 2 cut(s) 285, 339
BseBI CCWGG 2 cut(s) 11, 669
BseDI CCNNGG 1 cut(s) 10
BseGI GGATG 3 cut(s) 340, 917, 1384
BseJI GATNNNNATC 2 cut(s) 285, 339
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 2 cut(s) 268, 457
BseMII CTCAG 1 cut(s) 1251
BseNI ACTGG 6 cut(s) 75, 169, 488, 608, 1181, 1523
BseXI GCAGC 7 cut(s) 169, 662, 754, 776, 868, 1076, 1153
BseYI CCCAGC 1 cut(s) 130
BsgI GTGCAG 3 cut(s) 685, 867, 1497
BshNI GGYRCC 2 cut(s) 664, 1497
BshTI ACCGGT 1 cut(s) 704
BsiHKAI GWGCWC 1 cut(s) 895
BsiSI CCGG 3 cut(s) 705, 717, 851
BslFI GGGAC 3 cut(s) 163, 216, 569
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 966
BsmFI GGGAC 3 cut(s) 163, 216, 569
Bso31I GGTCTC 1 cut(s) 966
Bsp1286I GDGCHC 1 cut(s) 895
Bsp143I GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
BspACI CCGC 5 cut(s) 105, 292, 519, 653, 1289
BspCNI CTCAG 1 cut(s) 1252
BspLI GGNNCC 4 cut(s) 204, 666, 924, 1499
BspMAI CTGCAG 1 cut(s) 772
BspMI ACCTGC 1 cut(s) 125
BspPI GGATC 7 cut(s) 495, 614, 917, 930, 1027, 1235, 1280
BspT107I GGYRCC 2 cut(s) 664, 1497
BspTNI GGTCTC 1 cut(s) 966
BsrDI GCAATG 2 cut(s) 268, 457
BsrFI RCCGGY 2 cut(s) 704, 850
BsrI ACTGG 6 cut(s) 75, 169, 488, 608, 1181, 1523
BssAI RCCGGY 2 cut(s) 704, 850
BssECI CCNNGG 1 cut(s) 10
BssMI GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
BssSI CACGAG 1 cut(s) 1116
Bst2BI CACGAG 1 cut(s) 1116
Bst2UI CCWGG 2 cut(s) 11, 669
Bst4CI ACNGT 2 cut(s) 257, 1450
Bst6I CTCTTC 1 cut(s) 482
BstAPI GCANNNNNTGC 1 cut(s) 1070
BstC8I GCNNGC 1 cut(s) 1099
BstDEI CTNAG 3 cut(s) 386, 887, 1260
BstF5I GGATG 3 cut(s) 340, 917, 1384
BstKTI GATC 7 cut(s) 490, 609, 925, 1022, 1210, 1243, 1288
BstMAI GTCTC 1 cut(s) 966
BstMBI GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
BstMWI GCNNNNNNNGC 6 cut(s) 662, 764, 890, 1061, 1070, 1495
BstNI CCWGG 2 cut(s) 11, 669
BstNSI RCATGY 1 cut(s) 907
BstSCI CCNGG 2 cut(s) 9, 667
BstSFI CTRYAG 1 cut(s) 768
BstV1I GCAGC 7 cut(s) 169, 662, 754, 776, 868, 1076, 1153
BstX2I RGATCY 3 cut(s) 487, 922, 1207
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 3 cut(s) 487, 922, 1207
BsuI GTATCC 2 cut(s) 211, 1391
BtsCI GGATG 3 cut(s) 340, 917, 1384
BtsI GCAGTG 1 cut(s) 1412
BtsIMutI CAGTG 5 cut(s) 82, 481, 601, 1412, 1516
BveI ACCTGC 1 cut(s) 125
Cac8I GCNNGC 1 cut(s) 1099
Cfr10I RCCGGY 2 cut(s) 704, 850
Cfr13I GGNCC 3 cut(s) 203, 360, 1157
CseI GACGC 1 cut(s) 681
Csp6I GTAC 2 cut(s) 720, 1279
CspAI ACCGGT 1 cut(s) 704
CviAII CATG 6 cut(s) 325, 357, 761, 904, 955, 1407
CviQI GTAC 2 cut(s) 720, 1279
DdeI CTNAG 3 cut(s) 386, 887, 1260
DpnI GATC 7 cut(s) 489, 608, 924, 1021, 1209, 1242, 1287
DpnII GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
Eam1104I CTCTTC 1 cut(s) 482
EarI CTCTTC 1 cut(s) 482
Ecl136II GAGCTC 1 cut(s) 893
Eco24I GRGCYC 1 cut(s) 895
Eco31I GGTCTC 1 cut(s) 966
Eco32I GATATC 1 cut(s) 236
Eco47I GGWCC 3 cut(s) 203, 360, 1157
Eco53kI GAGCTC 1 cut(s) 893
Eco57I CTGAAG 1 cut(s) 1155
EcoICRI GAGCTC 1 cut(s) 893
EcoRI GAATTC 3 cut(s) 1146, 1200, 1435
EcoRII CCWGG 2 cut(s) 9, 667
EcoRV GATATC 1 cut(s) 236
EcoT22I ATGCAT 1 cut(s) 340
EcoT38I GRGCYC 1 cut(s) 895
FaeI CATG 6 cut(s) 328, 360, 764, 907, 958, 1410
FalI AAGNNNNNCTT 2 cut(s) 1296, 1328
FaqI GGGAC 3 cut(s) 163, 216, 569
FatI CATG 6 cut(s) 324, 356, 760, 903, 954, 1406
FauNDI CATATG 1 cut(s) 212
Fnu4HI GCNGC 8 cut(s) 183, 651, 654, 765, 768, 882, 1065, 1167
FokI GGATG 3 cut(s) 347, 924, 1391
FriOI GRGCYC 1 cut(s) 895
Fsp4HI GCNGC 8 cut(s) 183, 651, 654, 765, 768, 882, 1065, 1167
FspBI CTAG 6 cut(s) 123, 830, 896, 965, 1418, 1494
GluI GCNGC 8 cut(s) 183, 651, 654, 765, 768, 882, 1065, 1167
GsaI CCCAGC 1 cut(s) 134
HapII CCGG 3 cut(s) 705, 717, 851
HgaI GACGC 1 cut(s) 681
Hin1II CATG 6 cut(s) 328, 360, 764, 907, 958, 1410
HinfI GANTC 4 cut(s) 411, 713, 1329, 1461
HpaII CCGG 3 cut(s) 705, 717, 851
HphI GGTGA 2 cut(s) 281, 599
Hpy166II GTNNAC 1 cut(s) 400
Hpy188I TCNGA 5 cut(s) 682, 859, 1174, 1454, 1536
Hpy188III TCNNGA 5 cut(s) 188, 239, 363, 896, 1205
Hpy8I GTNNAC 1 cut(s) 400
HpyAV CCTTC 2 cut(s) 170, 1370
HpyCH4III ACNGT 2 cut(s) 257, 1450
HpyCH4IV ACGT 3 cut(s) 611, 1185, 1544
HpyF10VI GCNNNNNNNGC 6 cut(s) 662, 764, 890, 1061, 1070, 1495
HpyF3I CTNAG 3 cut(s) 386, 887, 1260
HpySE526I ACGT 3 cut(s) 611, 1185, 1544
Hsp92II CATG 6 cut(s) 328, 360, 764, 907, 958, 1410
Kzo9I GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
LmnI GCTCC 2 cut(s) 756, 997
Lsp1109I GCAGC 7 cut(s) 169, 662, 754, 776, 868, 1076, 1153
LweI GCATC 2 cut(s) 325, 1102
MaeI CTAG 6 cut(s) 123, 830, 896, 965, 1418, 1494
MaeII ACGT 3 cut(s) 611, 1185, 1544
MaeIII GTNAC 5 cut(s) 369, 469, 572, 587, 1410
MalI GATC 7 cut(s) 489, 608, 924, 1021, 1209, 1242, 1287
MboI GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
MboII GAAGA 6 cut(s) 314, 331, 469, 482, 518, 1316
MflI RGATCY 3 cut(s) 487, 922, 1207
MhlI GDGCHC 1 cut(s) 895
MlyI GAGTC 1 cut(s) 420
MmeI TCCRAC 2 cut(s) 312, 949
MnlI CCTC 7 cut(s) 122, 485, 543, 853, 951, 1086, 1190
Mph1103I ATGCAT 1 cut(s) 340
MseI TTAA 7 cut(s) 249, 527, 615, 1013, 1106, 1140, 1314
MslI CAYNNNNRTG 1 cut(s) 876
MspA1I CMGCKG 2 cut(s) 653, 767
MspI CCGG 3 cut(s) 705, 717, 851
MspR9I CCNGG 2 cut(s) 11, 669
MvaI CCWGG 2 cut(s) 11, 669
MwoI GCNNNNNNNGC 6 cut(s) 662, 764, 890, 1061, 1070, 1495
NdeI CATATG 1 cut(s) 212
NdeII GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
NlaIII CATG 6 cut(s) 328, 360, 764, 907, 958, 1410
NlaIV GGNNCC 4 cut(s) 204, 666, 924, 1499
NmuCI GTSAC 3 cut(s) 469, 587, 1410
NsiI ATGCAT 1 cut(s) 340
NspI RCATGY 1 cut(s) 907
PfeI GAWTC 3 cut(s) 713, 1329, 1461
PinAI ACCGGT 1 cut(s) 704
PkrI GCNGC 8 cut(s) 184, 652, 655, 766, 769, 883, 1066, 1168
PleI GAGTC 1 cut(s) 419
PpsI GAGTC 1 cut(s) 419
Psp124BI GAGCTC 1 cut(s) 895
Psp6I CCWGG 2 cut(s) 9, 667
PspFI CCCAGC 1 cut(s) 130
PspGI CCWGG 2 cut(s) 9, 667
PspN4I GGNNCC 4 cut(s) 204, 666, 924, 1499
PspPI GGNCC 3 cut(s) 203, 360, 1157
PstI CTGCAG 1 cut(s) 772
PsuI RGATCY 3 cut(s) 487, 922, 1207
PvuII CAGCTG 1 cut(s) 767
RsaI GTAC 2 cut(s) 721, 1280
RsaNI GTAC 2 cut(s) 720, 1279
RseI CAYNNNNRTG 1 cut(s) 876
SacI GAGCTC 1 cut(s) 895
SaqAI TTAA 7 cut(s) 249, 527, 615, 1013, 1106, 1140, 1314
SatI GCNGC 8 cut(s) 183, 651, 654, 765, 768, 882, 1065, 1167
Sau3AI GATC 7 cut(s) 487, 606, 922, 1019, 1207, 1240, 1285
Sau96I GGNCC 3 cut(s) 203, 360, 1157
SchI GAGTC 1 cut(s) 420
ScrFI CCNGG 2 cut(s) 11, 669
SduI GDGCHC 1 cut(s) 895
SfaNI GCATC 2 cut(s) 325, 1102
SfcI CTRYAG 1 cut(s) 768
SgrAI CRCCGGYG 1 cut(s) 704
SinI GGWCC 3 cut(s) 203, 360, 1157
SmiMI CAYNNNNRTG 1 cut(s) 876
SmlI CTYRAG 1 cut(s) 186
SmoI CTYRAG 1 cut(s) 186
SpeI ACTAGT 1 cut(s) 122
SsiI CCGC 5 cut(s) 105, 292, 519, 653, 1289
SspMI CTAG 6 cut(s) 123, 830, 896, 965, 1418, 1494
SstI GAGCTC 1 cut(s) 895
StyD4I CCNGG 2 cut(s) 9, 667
TaaI ACNGT 2 cut(s) 257, 1450
TaiI ACGT 3 cut(s) 614, 1188, 1547
TaqI TCGA 2 cut(s) 1204, 1332
TatI WGTACW 1 cut(s) 1278
TauI GCSGC 1 cut(s) 656
TfiI GAWTC 3 cut(s) 713, 1329, 1461
Tru1I TTAA 7 cut(s) 249, 527, 615, 1013, 1106, 1140, 1314
Tru9I TTAA 7 cut(s) 249, 527, 615, 1013, 1106, 1140, 1314
TscAI CASTG 5 cut(s) 82, 488, 608, 1419, 1523
TseFI GTSAC 3 cut(s) 469, 587, 1410
TseI GCWGC 7 cut(s) 182, 650, 764, 767, 881, 1064, 1166
Tsp45I GTSAC 3 cut(s) 469, 587, 1410
TspDTI ATGAA 8 cut(s) 17, 130, 147, 189, 297, 332, 453, 1119
TspRI CASTG 5 cut(s) 82, 488, 608, 1419, 1523
VpaK11BI GGWCC 3 cut(s) 203, 360, 1157
XapI RAATTY 5 cut(s) 581, 986, 1146, 1200, 1435
XbaI TCTAGA 1 cut(s) 895
XceI RCATGY 1 cut(s) 907
XspI CTAG 6 cut(s) 123, 830, 896, 965, 1418, 1494
Zsp2I ATGCAT 1 cut(s) 340
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.