RchiOBHm_Chr5g0077961

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000117
Physical Location & Seq
Forward (+)
83817068 .. 83821835
4768 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35251

Sequence Viewer

Length: 1626 bp
ATGTCATCCGTGTTTATCTCTAATGTCACGTTTCCCCTCTATATAAAGGGGGCCAAGTATAGAGCCAGAAAACATAGTCGATCAGTTATGGCACTTCAAGGCACTCTCATCTCTGCAATCATACTTGTTTTTAGCTGTGCAGTGGCAACTAGTATAGCTACCGCCCCAAGCCATTACGACGTTGCTTCCATCAACCGGAGCACCTTTCCGGCAGGTTTCATATTTGGGACAGCTTCTTCAGCTTACCAGTTCGAAGGTGCTGCAAAAGAAGATGGAAGAGGACCAAGCATATGGGACACATACACTCACAAAATTCCAGATAAGATAAAAGACCGCAGCACTGGAGATGTCGCTATTGATGCATATCACCGCTACAAGGAGGATGTTGGCATCATGAAAAATATGGGTTTTGATGCTTACAGATTCTCCATCTCGTGGTCTAGATTGTTACCAAATGGAACGCTAAGAGGGGGTGTGAATAAGGAAGGAATCAAATATTACAACAACCTCATAAACGAGCTTCTAGCCAATGGTCTAAAGCCCTTTGTCACACTCTTCCATTGGGATCTTCCCCAAGCTTTAGAAGATGAATACGGTGGCTTCTTAAGCCCTCAAATTGTAAACCATTTTCAAGACTACGCAGAGCTATGCTTCAAGGAATTTGGTGATAGAGTTAAGGACTGGATCACATTAAACGAGCCCTGGAGCTACGCCAATGGTGGTTATGTAATTGGGACGTTTGCACCATGCCGATGTTCTGAATGGCAAAACCTAAATTGCACCGGTGGAAATTCAGGAACAGAGCCATATTTGGTCTCACACTACCAGCTTCTAGCTCACGCAGCAGCCGTAAAGTTATACAAGGAGAAGTATCAGGCTGATCAGAAGGGTGTGATAGGGATAACAATACTGTCGCACTGGTTTGTGCCCTTTTCTGATGCCAAGCACCATGAAGAAGCTGCTCTACGAGCATTGGATTTTATGTTTGGATGGTATATGGATCCCTTAACAAACGGTGAATATCCGCACAGCATGAGATCTCTTGTTGGAGATCGATTACCCAAGTTCACTAAAGAGCAATCCAAGATGTTAAAAGGTTCATTTGATTTTCTGGGATTGAATTACTACACCGCTAACTATGCTACTTATGCACCTCATCTCAAGAATGCTGCAAACCCTAGCTACTTTACAGATGCTGTCGCTACTGTTTCCACCGAGCGTAATGGTATTCCTATTGGTCAAAAGGCTGCTTCAGATTGGCTCTATGTTTATCCAGAAGGAATTCGAGATCTATTGCTTTACACGAAGGAAAAGTACAATAATCCACTTATTTACATCACTGAAAATGGAAGGGATGAGCACAATGATCCCAAATTATCACTTGAGGAAGTCCTAGCCGATACTCACCGAATCGACTTTTACTATCGACATCTTTATTATCTTCATGAAGCGATCAAGGATGGCGTTAACGTGAAGGGATATTTTGCTTGGTCTTTGTTTGACAACTTCGAATGGAATATGGGTTACAGTGTTAGGTTTGGCATCAACTATGTGGATTACAATGATGGGCTTAAAAGATACCCTAAACTCTCAGCACACTGGTTCAAGAATTTTCTTGAAAATTAG

Protein Analysis

541

Amino Acids

61.71

Weight (kDa)

6.66

Isoelectric Point (pI)

28.56

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 66 - 540 1e-159 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 203
AccB7I CCANNNNNTGG 1 cut(s) 435
AciI CCGC 5 cut(s) 162, 334, 370, 1025, 1131
AclWI GGATC 5 cut(s) 573, 692, 995, 1008, 1361
AcsI RAATTY 5 cut(s) 312, 659, 790, 1281, 1609
AcuI CTGAAG 2 cut(s) 222, 1236
AfaI GTAC 1 cut(s) 1316
AfiI CCNNNNNNNGG 3 cut(s) 195, 376, 435
AflII CTTAAG 1 cut(s) 604
AgeI ACCGGT 1 cut(s) 782
AgsI TTSAA 6 cut(s) 98, 632, 655, 1120, 1606, 1619
AhlI ACTAGT 1 cut(s) 149
AjnI CCWGG 1 cut(s) 701
Alw21I GWGCWC 2 cut(s) 203, 1362
Alw26I GTCTC 1 cut(s) 820
AlwI GGATC 5 cut(s) 573, 692, 995, 1008, 1361
AlwNI CAGNNNCTG 1 cut(s) 1196
AoxI GGCC 1 cut(s) 51
ApeKI GCWGC 7 cut(s) 260, 336, 842, 845, 959, 1169, 1247
ApoI RAATTY 5 cut(s) 312, 659, 790, 1281, 1609
AsiGI ACCGGT 1 cut(s) 782
Asp700I GAANNNNTTC 1 cut(s) 1281
AspS9I GGNCC 2 cut(s) 51, 281
AsuHPI GGTGA 4 cut(s) 359, 677, 1028, 1397
AsuII TTCGAA 2 cut(s) 252, 1509
AvaII GGWCC 1 cut(s) 281
BaeGI GKGCMC 1 cut(s) 930
BamHI GGATCC 1 cut(s) 1000
BanII GRGCYC 1 cut(s) 702
BarI GAAGNNNNNNTAC 4 cut(s) 948, 980, 1298, 1330
BauI CACGAG 1 cut(s) 433
Bbv12I GWGCWC 2 cut(s) 203, 1362
BbvI GCAGC 7 cut(s) 247, 348, 854, 857, 946, 1156, 1234
BccI CCATC 6 cut(s) 197, 266, 437, 984, 1454, 1559
BceAI ACGGC 1 cut(s) 833
BcgI CGANNNNNNTGC 2 cut(s) 242, 276
BciT130I CCWGG 1 cut(s) 703
BclI TGATCA 1 cut(s) 880
BcoDI GTCTC 1 cut(s) 820
BcuI ACTAGT 1 cut(s) 149
BfaI CTAG 6 cut(s) 150, 441, 524, 833, 1179, 1394
BfrI CTTAAG 1 cut(s) 604
BfuAI ACCTGC 1 cut(s) 203
BglII AGATCT 2 cut(s) 1037, 1288
BisI GCNGC 7 cut(s) 261, 337, 843, 846, 960, 1170, 1248
BlsI GCNGC 7 cut(s) 262, 338, 844, 847, 961, 1171, 1249
Bme1390I CCNGG 1 cut(s) 703
Bme18I GGWCC 1 cut(s) 281
BmgT120I GGNCC 2 cut(s) 51, 281
BmiI GGNNCC 2 cut(s) 52, 1002
BmrFI CCNGG 1 cut(s) 703
BmsI GCATC 6 cut(s) 349, 399, 403, 928, 1183, 1551
BpmI CTGGAG 2 cut(s) 363, 724
Bpu14I TTCGAA 2 cut(s) 252, 1509
BpuEI CTTGAG 2 cut(s) 1145, 1403
Bsa29I ATCGAT 1 cut(s) 1054
BsaBI GATNNNNATC 1 cut(s) 363
BsaI GGTCTC 1 cut(s) 820
BsaJI CCNNGG 1 cut(s) 701
BsaWI WCCGGW 2 cut(s) 195, 782
BsaXI ACNNNNNCTCC 2 cut(s) 410, 440
Bsc4I CCNNNNNNNGG 3 cut(s) 195, 376, 435
Bse118I RCCGGY 1 cut(s) 782
Bse1I ACTGG 5 cut(s) 247, 346, 686, 923, 1604
Bse8I GATNNNNATC 1 cut(s) 363
BseBI CCWGG 1 cut(s) 703
BseCI ATCGAT 1 cut(s) 1054
BseDI CCNNGG 1 cut(s) 701
BseGI GGATG 5 cut(s) 5, 388, 995, 1360, 1465
BseJI GATNNNNATC 1 cut(s) 363
BseLI CCNNNNNNNGG 3 cut(s) 195, 376, 435
BseMII CTCAG 1 cut(s) 1605
BseNI ACTGG 5 cut(s) 247, 346, 686, 923, 1604
BseSI GKGCMC 1 cut(s) 930
BseXI GCAGC 7 cut(s) 247, 348, 854, 857, 946, 1156, 1234
BsgI GTGCAG 1 cut(s) 159
BshFI GGCC 1 cut(s) 53
BshTI ACCGGT 1 cut(s) 782
BshVI ATCGAT 1 cut(s) 1054
BsiHKAI GWGCWC 2 cut(s) 203, 1362
BsiSI CCGG 3 cut(s) 196, 209, 783
BslFI GGGAC 3 cut(s) 241, 308, 748
BslI CCNNNNNNNGG 3 cut(s) 195, 376, 435
BsmAI GTCTC 1 cut(s) 820
BsmFI GGGAC 3 cut(s) 241, 308, 748
BsmI GAATGC 1 cut(s) 1171
BsnI GGCC 1 cut(s) 53
Bso31I GGTCTC 1 cut(s) 820
Bsp119I TTCGAA 2 cut(s) 252, 1509
Bsp1286I GDGCHC 4 cut(s) 203, 702, 930, 1362
BspACI CCGC 5 cut(s) 162, 334, 370, 1025, 1131
BspANI GGCC 1 cut(s) 53
BspCNI CTCAG 1 cut(s) 1604
BspDI ATCGAT 1 cut(s) 1054
BspHI TCATGA 2 cut(s) 393, 1444
BspLI GGNNCC 2 cut(s) 52, 1002
BspMI ACCTGC 1 cut(s) 203
BspPI GGATC 5 cut(s) 573, 692, 995, 1008, 1361
BspT104I TTCGAA 2 cut(s) 252, 1509
BspTI CTTAAG 1 cut(s) 604
BspTNI GGTCTC 1 cut(s) 820
BsrFI RCCGGY 1 cut(s) 782
BsrI ACTGG 5 cut(s) 247, 346, 686, 923, 1604
BssAI RCCGGY 1 cut(s) 782
BssECI CCNNGG 1 cut(s) 701
BssSI CACGAG 1 cut(s) 433
Bst2BI CACGAG 1 cut(s) 433
Bst2UI CCWGG 1 cut(s) 703
Bst4CI ACNGT 5 cut(s) 596, 912, 1016, 1207, 1529
Bst6I CTCTTC 2 cut(s) 271, 560
BstAFI CTTAAG 1 cut(s) 604
BstBI TTCGAA 2 cut(s) 252, 1509
BstDEI CTNAG 2 cut(s) 464, 1591
BstF5I GGATG 5 cut(s) 5, 388, 995, 1360, 1465
BstMAI GTCTC 1 cut(s) 820
BstMWI GCNNNNNNNGC 7 cut(s) 239, 359, 606, 842, 968, 1139, 1148
BstNI CCWGG 1 cut(s) 703
BstSCI CCNGG 1 cut(s) 701
BstSLI GKGCMC 1 cut(s) 930
BstV1I GCAGC 7 cut(s) 247, 348, 854, 857, 946, 1156, 1234
BstX2I RGATCY 4 cut(s) 565, 1000, 1037, 1288
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 4 cut(s) 565, 1000, 1037, 1288
Bsu15I ATCGAT 1 cut(s) 1054
BsuRI GGCC 1 cut(s) 53
BsuTUI ATCGAT 1 cut(s) 1054
BtsCI GGATG 5 cut(s) 5, 388, 995, 1360, 1465
BtsI GCAGTG 1 cut(s) 147
BtsIMutI CAGTG 6 cut(s) 147, 339, 916, 1338, 1534, 1597
BveI ACCTGC 1 cut(s) 203
CaiI CAGNNNCTG 1 cut(s) 1196
CciI TCATGA 2 cut(s) 393, 1444
Cfr10I RCCGGY 1 cut(s) 782
Cfr13I GGNCC 2 cut(s) 51, 281
ClaI ATCGAT 1 cut(s) 1054
Csp6I GTAC 1 cut(s) 1315
CspAI ACCGGT 1 cut(s) 782
CviAII CATG 5 cut(s) 394, 747, 950, 1033, 1445
CviQI GTAC 1 cut(s) 1315
DdeI CTNAG 2 cut(s) 464, 1591
Eam1104I CTCTTC 2 cut(s) 271, 560
EarI CTCTTC 2 cut(s) 271, 560
Eco24I GRGCYC 1 cut(s) 702
Eco31I GGTCTC 1 cut(s) 820
Eco47I GGWCC 1 cut(s) 281
Eco57I CTGAAG 2 cut(s) 222, 1236
EcoRI GAATTC 1 cut(s) 1281
EcoRII CCWGG 1 cut(s) 701
EcoT22I ATGCAT 1 cut(s) 364
EcoT38I GRGCYC 1 cut(s) 702
FaeI CATG 5 cut(s) 397, 750, 953, 1036, 1448
FaqI GGGAC 3 cut(s) 241, 308, 748
FatI CATG 5 cut(s) 393, 746, 949, 1032, 1444
FauNDI CATATG 1 cut(s) 290
FbaI TGATCA 1 cut(s) 880
Fnu4HI GCNGC 7 cut(s) 261, 337, 843, 846, 960, 1170, 1248
FokI GGATG 4 cut(s) 395, 1002, 1367, 1472
FriOI GRGCYC 1 cut(s) 702
Fsp4HI GCNGC 7 cut(s) 261, 337, 843, 846, 960, 1170, 1248
FspBI CTAG 6 cut(s) 150, 441, 524, 833, 1179, 1394
GluI GCNGC 7 cut(s) 261, 337, 843, 846, 960, 1170, 1248
GsuI CTGGAG 2 cut(s) 363, 724
HaeIII GGCC 1 cut(s) 53
HapII CCGG 3 cut(s) 196, 209, 783
Hin1II CATG 5 cut(s) 397, 750, 953, 1036, 1448
HincII GTYRAC 1 cut(s) 1468
HindII GTYRAC 1 cut(s) 1468
HindIII AAGCTT 1 cut(s) 576
HinfI GANTC 3 cut(s) 423, 489, 1410
HpaI GTTAAC 1 cut(s) 1468
HpaII CCGG 3 cut(s) 196, 209, 783
HphI GGTGA 4 cut(s) 359, 677, 1028, 1397
Hpy166II GTNNAC 3 cut(s) 622, 1068, 1468
Hpy188I TCNGA 4 cut(s) 760, 885, 937, 1255
Hpy8I GTNNAC 3 cut(s) 622, 1068, 1468
Hpy99I CGWCG 1 cut(s) 182
HpyAV CCTTC 7 cut(s) 248, 479, 880, 1271, 1300, 1344, 1468
HpyCH4III ACNGT 5 cut(s) 596, 912, 1016, 1207, 1529
HpyCH4IV ACGT 4 cut(s) 29, 180, 737, 1470
HpyCH4V TGCA 8 cut(s) 116, 140, 263, 362, 743, 780, 1151, 1172
HpyF10VI GCNNNNNNNGC 7 cut(s) 239, 359, 606, 842, 968, 1139, 1148
HpyF3I CTNAG 2 cut(s) 464, 1591
HpySE526I ACGT 4 cut(s) 29, 180, 737, 1470
Hsp92II CATG 5 cut(s) 397, 750, 953, 1036, 1448
Ksp22I TGATCA 1 cut(s) 880
KspAI GTTAAC 1 cut(s) 1468
LmnI GCTCC 2 cut(s) 198, 705
Lsp1109I GCAGC 7 cut(s) 247, 348, 854, 857, 946, 1156, 1234
LweI GCATC 6 cut(s) 349, 399, 403, 928, 1183, 1551
MaeI CTAG 6 cut(s) 150, 441, 524, 833, 1179, 1394
MaeII ACGT 4 cut(s) 29, 180, 737, 1470
MaeIII GTNAC 4 cut(s) 25, 447, 547, 1523
MboII GAAGA 8 cut(s) 228, 281, 288, 547, 560, 596, 965, 1433
MflI RGATCY 4 cut(s) 565, 1000, 1037, 1288
MhlI GDGCHC 4 cut(s) 203, 702, 930, 1362
MmeI TCCRAC 1 cut(s) 1027
MnlI CCTC 8 cut(s) 47, 272, 373, 461, 518, 621, 1164, 1378
Mph1103I ATGCAT 1 cut(s) 364
MroXI GAANNNNTTC 1 cut(s) 1281
MseI TTAA 7 cut(s) 605, 675, 692, 1007, 1091, 1467, 1572
MslI CAYNNNNRTG 1 cut(s) 751
MspCI CTTAAG 1 cut(s) 604
MspI CCGG 3 cut(s) 196, 209, 783
MspR9I CCNGG 1 cut(s) 703
Mva1269I GAATGC 1 cut(s) 1171
MvaI CCWGG 1 cut(s) 703
MwoI GCNNNNNNNGC 7 cut(s) 239, 359, 606, 842, 968, 1139, 1148
NdeI CATATG 1 cut(s) 290
NlaIII CATG 5 cut(s) 397, 750, 953, 1036, 1448
NlaIV GGNNCC 2 cut(s) 52, 1002
NmuCI GTSAC 2 cut(s) 25, 547
NsiI ATGCAT 1 cut(s) 364
NspV TTCGAA 2 cut(s) 252, 1509
PagI TCATGA 2 cut(s) 393, 1444
PcsI WCGNNNNNNNCGW 1 cut(s) 846
PctI GAATGC 1 cut(s) 1171
PdmI GAANNNNTTC 1 cut(s) 1281
PfeI GAWTC 3 cut(s) 423, 489, 1410
PflMI CCANNNNNTGG 1 cut(s) 435
PinAI ACCGGT 1 cut(s) 782
PkrI GCNGC 7 cut(s) 262, 338, 844, 847, 961, 1171, 1249
Psp6I CCWGG 1 cut(s) 701
PspGI CCWGG 1 cut(s) 701
PspN4I GGNNCC 2 cut(s) 52, 1002
PspPI GGNCC 2 cut(s) 51, 281
PstNI CAGNNNCTG 1 cut(s) 1196
PsuI RGATCY 4 cut(s) 565, 1000, 1037, 1288
RsaI GTAC 1 cut(s) 1316
RsaNI GTAC 1 cut(s) 1315
RseI CAYNNNNRTG 1 cut(s) 751
SaqAI TTAA 7 cut(s) 605, 675, 692, 1007, 1091, 1467, 1572
SatI GCNGC 7 cut(s) 261, 337, 843, 846, 960, 1170, 1248
Sau96I GGNCC 2 cut(s) 51, 281
ScrFI CCNGG 1 cut(s) 703
SduI GDGCHC 4 cut(s) 203, 702, 930, 1362
SfaNI GCATC 6 cut(s) 349, 399, 403, 928, 1183, 1551
SfuI TTCGAA 2 cut(s) 252, 1509
SgrAI CRCCGGYG 1 cut(s) 782
SinI GGWCC 1 cut(s) 281
SmiMI CAYNNNNRTG 1 cut(s) 751
SmlI CTYRAG 3 cut(s) 604, 1160, 1382
SmoI CTYRAG 3 cut(s) 604, 1160, 1382
SpeI ACTAGT 1 cut(s) 149
SsiI CCGC 5 cut(s) 162, 334, 370, 1025, 1131
SspI AATATT 1 cut(s) 497
SspMI CTAG 6 cut(s) 150, 441, 524, 833, 1179, 1394
StyD4I CCNGG 1 cut(s) 701
TaaI ACNGT 5 cut(s) 596, 912, 1016, 1207, 1529
TaiI ACGT 4 cut(s) 32, 183, 740, 1473
TaqI TCGA 7 cut(s) 79, 252, 1054, 1285, 1413, 1426, 1509
TatI WGTACW 1 cut(s) 1314
TfiI GAWTC 3 cut(s) 423, 489, 1410
Tru1I TTAA 7 cut(s) 605, 675, 692, 1007, 1091, 1467, 1572
Tru9I TTAA 7 cut(s) 605, 675, 692, 1007, 1091, 1467, 1572
TscAI CASTG 6 cut(s) 147, 346, 923, 1345, 1534, 1604
TseFI GTSAC 2 cut(s) 25, 547
TseI GCWGC 7 cut(s) 260, 336, 842, 845, 959, 1169, 1247
Tsp45I GTSAC 2 cut(s) 25, 547
TspDTI ATGAA 7 cut(s) 208, 410, 603, 966, 1089, 1433, 1461
TspRI CASTG 6 cut(s) 147, 346, 923, 1345, 1534, 1604
Van91I CCANNNNNTGG 1 cut(s) 435
Vha464I CTTAAG 1 cut(s) 604
VpaK11BI GGWCC 1 cut(s) 281
XapI RAATTY 5 cut(s) 312, 659, 790, 1281, 1609
XbaI TCTAGA 1 cut(s) 440
XmnI GAANNNNTTC 1 cut(s) 1281
XspI CTAG 6 cut(s) 150, 441, 524, 833, 1179, 1394
Zsp2I ATGCAT 1 cut(s) 364
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.