MD03G1069500.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000117
Physical Location & Seq
Forward (+)
5680476 .. 5684362
3887 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1069500.v1.1.491

Sequence Viewer

Length: 1950 bp
ATGTTCAGACTTAACTCGGTGCCCCTCCCGACTCTGTCCCAACGGTCAGCCATGGCCGCCATCCACTCCATAGCAGCTCAGAGCTTCCTCTCTCTCCCGAACCCCAAACCCAGAAAACGCATTGCCGCTCACCCAAAACCCATCTTGACCTCCAAACTCCCAAAAAACCCAACTTTCCCCAGAAACAAACAGAAAGGCAGGAGGGTTTTGAACTCGGCGGTCAAGCGAGAAGAGTGCGATGTGATTCCGGTTCAGAGCTCCGACAGTACGGACCAGCAGGAAGGGGTGGTGGCCAGCAGGGTGGAGAGCGAAGCCGGGGATCAGGGGGAGTTGGTGAGTCAGGTGGGCGGGTTCGGAGCGAGTGAGGGGAGGCTTTCGTTTGAAGGGGCTGGTGGGTTTGGGTCTACTGGGGTTGGAAGTGAGAGGGAGAGTGAGGAATTCGAGAGGCCGGTGGAAAAACTACTAACTAATATACCTAGTCGTTATAGCAGTGCTTCCCTGAACAGAAGCAGTGCTTCCCTGAACAGGAGCAGTTTTCCATCAGGCTTTGTATTTGGTTCAGCTTCAGCATCTTACCAATATGAAGGTGCATGGAATGAAGGTGGTAAAGGACCAAGCATATGGGATAACTTCACCCACCAGTATCCAGAAAAAATCAGTGATGGAAGCAATGGGGACGTGGCTAATGATCAATACCACCGCTATAAGGAAGATGTAAAGATTATGAAGGATATGGGATTGGATGCTTATAGGTTCTCTATCTCATGGTCCAGATTGTTACCAAATGGAAAGCTAAGTGGGGGTGTGAGCAAGGAAGGAGTACAATACTACAACAATCTCATCAATGAACTCCAAAACAAAGGTATAGCGCCATATGTGACAATCTTTCATTGGGATCTTCCTCAAGCTTTAGAAGAAGAATATGGTGGTTTCTTAAACCGTCAAATTGTCAATCATTTTCGAGACTATGCAGAACTTTGCTTTAAGTTATTTGGCGATCGGGTAAAGCATTGGATCACACTAAATGAGCCATATAATTTTATTAATTTTGGCTATGCATCAGGACAATTAGCACCCGGGCGATGTTCTGCTTGGCAGAACTTAAACTGCACCGGCGGTGATTCGGCTACCGAACCCTATATAGTAGCACACCACTTCCTCCTCGCTCATGCACATGCTGTTGAAGTGTACAAGACTAAATATCAGGCATCTCAAGAAGGCGTGATAGGAATAACATTAGCGGCAAATTGGTTTGTGCCAATTTCTAACGAAACTCGTCATCAGAATGCTGCAAATCGATCTTTGGATTTTATGTTTGGATGGTTTATGGAGCCATTGACAAGCGGCCAATATCCGCACAGTATGCAAGTTCTTGTTAAAGAAAGATTACCTAAATTTACAGAAGAAGAATCCAAGTTAATAAAAGGGTCATTTGATTTTGTTGGAATGAATTATTATACTACTCACTATTCAAGCGATCAACCTGATAATAATTCTGCAAATCCAAGCTTCTTGACCGATGCTTGCGTTTTTGAATCAACCGAGCTTAATGGAGTCCCCATTGGTCCTCCGGCTGCTTCAAGCTGGCTAGTTATTTATCCAAAAGGCATTCGAGAGATTTTACTTTACGCAAAGCACAAATATAATAATCCGGTCATTTACATTACTGAGAACGGCATGGATGAGTTCGATGATCCCAAATTGTCACTTCCGCAATCCCTCAATGATACCCACAGAATTGATTACCACTACCACCACCTCGACTATCTTCGAAAAGCAATCGATGATGGTGTAAATGTGAAGGGATACTTTGCATGGTCATTGACGGACAATTTTGAATGGGCTGCTGGATACACCTTACGATTTGGTTTCGTCTATATAGATTACAATGATGGACTTAAGAGGCACCCAAAACTCTCAGCAAGCTGGTTCAAATATTTCCTTGGATAA

Protein Analysis

650

Amino Acids

72.91

Weight (kDa)

6.3

Isoelectric Point (pI)

39.2

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 177 - 646 4.1e-163 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 19, 1905
AccBSI CCGCTC 1 cut(s) 128
AccI GTMKAC 1 cut(s) 404
AclWI GGATC 4 cut(s) 327, 903, 1022, 1688
AcoI YGGCCR 3 cut(s) 54, 291, 1345
AcsI RAATTY 2 cut(s) 437, 1394
AcuI CTGAAG 1 cut(s) 549
AfaI GTAC 3 cut(s) 268, 822, 1190
AfiI CCNNNNNNNGG 2 cut(s) 525, 706
AflII CTTAAG 1 cut(s) 1898
AgsI TTSAA 8 cut(s) 211, 383, 1184, 1473, 1535, 1581, 1838, 1933
AjiI CACGTC 1 cut(s) 679
Alw21I GWGCWC 1 cut(s) 260
Alw26I GTCTC 1 cut(s) 957
AlwI GGATC 4 cut(s) 327, 903, 1022, 1688
Ama87I CYCGRG 1 cut(s) 1076
AoxI GGCC 4 cut(s) 54, 291, 446, 1345
ApeKI GCWGC 4 cut(s) 74, 1289, 1574, 1844
ApoI RAATTY 2 cut(s) 437, 1394
AseI ATTAAT 1 cut(s) 1044
AspLEI GCGC 1 cut(s) 871
AspS9I GGNCC 4 cut(s) 271, 611, 768, 1565
AsuC2I CCSGG 3 cut(s) 316, 1077, 1078
AsuHPI GGTGA 4 cut(s) 122, 346, 625, 1130
AsuII TTCGAA 1 cut(s) 1771
AvaI CYCGRG 1 cut(s) 1076
AvaII GGWCC 4 cut(s) 271, 611, 768, 1565
BaeGI GKGCMC 1 cut(s) 24
BalI TGGCCA 1 cut(s) 293
BanI GGYRCC 2 cut(s) 19, 1905
BanII GRGCYC 1 cut(s) 260
Bbv12I GWGCWC 1 cut(s) 260
BbvI GCAGC 4 cut(s) 86, 1276, 1561, 1831
BccI CCATC 7 cut(s) 68, 149, 547, 656, 1314, 1781, 1886
BceAI ACGGC 1 cut(s) 1690
BcgI CGANNNNNNTGC 2 cut(s) 216, 250
BciVI GTATCC 3 cut(s) 654, 1799, 1844
BclI TGATCA 1 cut(s) 688
BcnI CCSGG 3 cut(s) 316, 1077, 1078
BcoDI GTCTC 1 cut(s) 957
BfaI CTAG 2 cut(s) 477, 1589
BfoI RGCGCY 1 cut(s) 872
BfrI CTTAAG 1 cut(s) 1898
BfuI GTATCC 3 cut(s) 654, 1799, 1844
BisI GCNGC 8 cut(s) 57, 75, 126, 1242, 1290, 1345, 1575, 1845
BlsI GCNGC 8 cut(s) 58, 76, 127, 1243, 1291, 1346, 1576, 1846
Bme1390I CCNGG 3 cut(s) 316, 1077, 1078
Bme18I GGWCC 4 cut(s) 271, 611, 768, 1565
BmeT110I CYCGRG 1 cut(s) 1076
BmgBI CACGTC 1 cut(s) 679
BmgT120I GGNCC 4 cut(s) 271, 611, 768, 1565
BmiI GGNNCC 3 cut(s) 21, 1332, 1907
BmrFI CCNGG 3 cut(s) 316, 1077, 1078
BmrI ACTGGG 1 cut(s) 417
BmsI GCATC 5 cut(s) 578, 733, 1067, 1217, 1510
BmuI ACTGGG 1 cut(s) 417
Bpu14I TTCGAA 1 cut(s) 1771
BpuEI CTTGAG 2 cut(s) 888, 1197
BpuMI CCSGG 3 cut(s) 316, 1077, 1078
Bsa29I ATCGAT 2 cut(s) 1297, 1782
BsaJI CCNNGG 4 cut(s) 51, 315, 1076, 1942
BsaWI WCCGGW 2 cut(s) 247, 1651
Bsc4I CCNNNNNNNGG 2 cut(s) 525, 706
Bse118I RCCGGY 2 cut(s) 448, 1112
Bse1I ACTGG 2 cut(s) 412, 640
Bse3DI GCAATG 2 cut(s) 120, 676
BseCI ATCGAT 2 cut(s) 1297, 1782
BseDI CCNNGG 4 cut(s) 51, 315, 1076, 1942
BseGI GGATG 4 cut(s) 60, 748, 1325, 1687
BseLI CCNNNNNNNGG 2 cut(s) 525, 706
BseMI GCAATG 2 cut(s) 120, 676
BseMII CTCAG 3 cut(s) 92, 1659, 1932
BseNI ACTGG 2 cut(s) 412, 640
BseRI GAGGAG 1 cut(s) 1151
BseSI GKGCMC 1 cut(s) 24
BseXI GCAGC 4 cut(s) 86, 1276, 1561, 1831
BsgI GTGCAG 1 cut(s) 1093
Bsh1285I CGRYCG 1 cut(s) 1000
BshFI GGCC 4 cut(s) 56, 293, 448, 1347
BshNI GGYRCC 2 cut(s) 19, 1905
BshVI ATCGAT 2 cut(s) 1297, 1782
BsiEI CGRYCG 1 cut(s) 1000
BsiHKAI GWGCWC 1 cut(s) 260
BsiHKCI CYCGRG 1 cut(s) 1076
BsiSI CCGG 7 cut(s) 248, 315, 449, 1077, 1113, 1571, 1652
BslFI GGGAC 3 cut(s) 22, 689, 1541
BslI CCNNNNNNNGG 2 cut(s) 525, 706
BsmAI GTCTC 1 cut(s) 957
BsmFI GGGAC 3 cut(s) 22, 689, 1541
BsmI GAATGC 2 cut(s) 1291, 1608
BsnI GGCC 4 cut(s) 56, 293, 448, 1347
BsoBI CYCGRG 1 cut(s) 1076
Bsp119I TTCGAA 1 cut(s) 1771
Bsp1286I GDGCHC 2 cut(s) 24, 260
Bsp1407I TGTACA 1 cut(s) 1188
Bsp143I GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
Bsp19I CCATGG 1 cut(s) 51
BspANI GGCC 4 cut(s) 56, 293, 448, 1347
BspCNI CTCAG 3 cut(s) 91, 1660, 1931
BspDI ATCGAT 2 cut(s) 1297, 1782
BspLI GGNNCC 3 cut(s) 21, 1332, 1907
BspPI GGATC 4 cut(s) 327, 903, 1022, 1688
BspT104I TTCGAA 1 cut(s) 1771
BspT107I GGYRCC 2 cut(s) 19, 1905
BspTI CTTAAG 1 cut(s) 1898
BsrBI CCGCTC 1 cut(s) 128
BsrDI GCAATG 2 cut(s) 120, 676
BsrFI RCCGGY 2 cut(s) 448, 1112
BsrGI TGTACA 1 cut(s) 1188
BsrI ACTGG 2 cut(s) 412, 640
BssAI RCCGGY 2 cut(s) 448, 1112
BssECI CCNNGG 4 cut(s) 51, 315, 1076, 1942
BssMI GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
BssT1I CCWWGG 2 cut(s) 51, 1942
Bst4CI ACNGT 4 cut(s) 45, 266, 941, 1361
Bst6I CTCTTC 1 cut(s) 225
BstAFI CTTAAG 1 cut(s) 1898
BstAPI GCANNNNNTGC 1 cut(s) 1363
BstAUI TGTACA 1 cut(s) 1188
BstBI TTCGAA 1 cut(s) 1771
BstC8I GCNNGC 4 cut(s) 295, 1525, 1586, 1924
BstDEI CTNAG 4 cut(s) 78, 794, 1668, 1918
BstDSI CCRYGG 1 cut(s) 51
BstF5I GGATG 4 cut(s) 60, 748, 1325, 1687
BstH2I RGCGCY 1 cut(s) 872
BstHHI GCGC 1 cut(s) 871
BstKTI GATC 8 cut(s) 322, 691, 898, 1000, 1017, 1301, 1480, 1696
BstMAI GTCTC 1 cut(s) 957
BstMBI GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
BstMCI CGRYCG 1 cut(s) 1000
BstMWI GCNNNNNNNGC 2 cut(s) 56, 1363
BstNSI RCATGY 1 cut(s) 1178
BstSCI CCNGG 3 cut(s) 314, 1075, 1076
BstSLI GKGCMC 1 cut(s) 24
BstV1I GCAGC 4 cut(s) 86, 1276, 1561, 1831
BstX2I RGATCY 1 cut(s) 895
BstXI CCANNNNNNTGG 2 cut(s) 301, 621
BstYI RGATCY 1 cut(s) 895
Bsu15I ATCGAT 2 cut(s) 1297, 1782
BsuI GTATCC 3 cut(s) 654, 1799, 1844
BsuRI GGCC 4 cut(s) 56, 293, 448, 1347
BsuTUI ATCGAT 2 cut(s) 1297, 1782
BtgI CCRYGG 1 cut(s) 51
BtgZI GCGATG 2 cut(s) 252, 1096
BtrI CACGTC 1 cut(s) 679
BtsCI GGATG 4 cut(s) 60, 748, 1325, 1687
BtsI GCAGTG 2 cut(s) 496, 517
BtsIMutI CAGTG 3 cut(s) 496, 517, 664
Cac8I GCNNGC 4 cut(s) 295, 1525, 1586, 1924
CfoI GCGC 1 cut(s) 871
Cfr10I RCCGGY 2 cut(s) 448, 1112
Cfr13I GGNCC 4 cut(s) 271, 611, 768, 1565
Cfr9I CCCGGG 1 cut(s) 1076
ClaI ATCGAT 2 cut(s) 1297, 1782
Csp6I GTAC 3 cut(s) 267, 821, 1189
CviAII CATG 7 cut(s) 52, 591, 765, 1169, 1175, 1678, 1815
CviQI GTAC 3 cut(s) 267, 821, 1189
DdeI CTNAG 4 cut(s) 78, 794, 1668, 1918
DpnI GATC 8 cut(s) 321, 690, 897, 999, 1016, 1300, 1479, 1695
DpnII GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
EaeI YGGCCR 3 cut(s) 54, 291, 1345
Eam1104I CTCTTC 1 cut(s) 225
EarI CTCTTC 1 cut(s) 225
Ecl136II GAGCTC 1 cut(s) 258
Eco130I CCWWGG 2 cut(s) 51, 1942
Eco24I GRGCYC 1 cut(s) 260
Eco47I GGWCC 4 cut(s) 271, 611, 768, 1565
Eco53kI GAGCTC 1 cut(s) 258
Eco57I CTGAAG 1 cut(s) 549
Eco88I CYCGRG 1 cut(s) 1076
EcoICRI GAGCTC 1 cut(s) 258
EcoRI GAATTC 1 cut(s) 437
EcoT14I CCWWGG 2 cut(s) 51, 1942
EcoT22I ATGCAT 1 cut(s) 1060
EcoT38I GRGCYC 1 cut(s) 260
ErhI CCWWGG 2 cut(s) 51, 1942
FaeI CATG 7 cut(s) 55, 594, 768, 1172, 1178, 1681, 1818
FalI AAGNNNNNCTT 4 cut(s) 499, 531, 1793, 1825
FaqI GGGAC 3 cut(s) 22, 689, 1541
FatI CATG 7 cut(s) 51, 590, 764, 1168, 1174, 1677, 1814
FauI CCCGC 1 cut(s) 341
FauNDI CATATG 2 cut(s) 620, 874
FbaI TGATCA 1 cut(s) 688
FblI GTMKAC 1 cut(s) 404
Fnu4HI GCNGC 8 cut(s) 57, 75, 126, 1242, 1290, 1345, 1575, 1845
FokI GGATG 4 cut(s) 47, 755, 1332, 1694
FriOI GRGCYC 1 cut(s) 260
Fsp4HI GCNGC 8 cut(s) 57, 75, 126, 1242, 1290, 1345, 1575, 1845
FspBI CTAG 2 cut(s) 477, 1589
GlaI GCGC 1 cut(s) 870
GluI GCNGC 8 cut(s) 57, 75, 126, 1242, 1290, 1345, 1575, 1845
HaeII RGCGCY 1 cut(s) 872
HaeIII GGCC 4 cut(s) 56, 293, 448, 1347
HapII CCGG 7 cut(s) 248, 315, 449, 1077, 1113, 1571, 1652
HhaI GCGC 1 cut(s) 871
Hin1II CATG 7 cut(s) 55, 594, 768, 1172, 1178, 1681, 1818
Hin6I GCGC 1 cut(s) 869
HinP1I GCGC 1 cut(s) 869
HindIII AAGCTT 2 cut(s) 906, 1507
HinfI GANTC 7 cut(s) 31, 244, 337, 1121, 1409, 1535, 1554
HpaII CCGG 7 cut(s) 248, 315, 449, 1077, 1113, 1571, 1652
HphI GGTGA 4 cut(s) 122, 346, 625, 1130
Hpy166II GTNNAC 2 cut(s) 405, 1189
Hpy188I TCNGA 6 cut(s) 8, 81, 255, 262, 356, 1284
Hpy8I GTNNAC 2 cut(s) 405, 1189
HpyAV CCTTC 8 cut(s) 275, 377, 578, 593, 721, 809, 1211, 1795
HpyCH4III ACNGT 4 cut(s) 45, 266, 941, 1361
HpyCH4IV ACGT 1 cut(s) 678
HpyCH4V TGCA 9 cut(s) 590, 971, 1058, 1110, 1172, 1292, 1366, 1499, 1814
HpyF10VI GCNNNNNNNGC 2 cut(s) 56, 1363
HpyF3I CTNAG 4 cut(s) 78, 794, 1668, 1918
HpySE526I ACGT 1 cut(s) 678
Hsp92II CATG 7 cut(s) 55, 594, 768, 1172, 1178, 1681, 1818
HspAI GCGC 1 cut(s) 869
Ksp22I TGATCA 1 cut(s) 688
Kzo9I GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
LmnI GCTCC 4 cut(s) 263, 356, 528, 1330
Lsp1109I GCAGC 4 cut(s) 86, 1276, 1561, 1831
LweI GCATC 5 cut(s) 578, 733, 1067, 1217, 1510
MaeI CTAG 2 cut(s) 477, 1589
MaeII ACGT 1 cut(s) 678
MaeIII GTNAC 3 cut(s) 777, 877, 1704
MalI GATC 8 cut(s) 321, 690, 897, 999, 1016, 1300, 1479, 1695
MbiI CCGCTC 1 cut(s) 128
MboI GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
MboII GAAGA 8 cut(s) 242, 722, 890, 926, 929, 1415, 1418, 1760
MflI RGATCY 1 cut(s) 895
MhlI GDGCHC 2 cut(s) 24, 260
MlsI TGGCCA 1 cut(s) 293
MluNI TGGCCA 1 cut(s) 293
MlyI GAGTC 3 cut(s) 25, 346, 1563
MmeI TCCRAC 3 cut(s) 285, 394, 1423
Mox20I TGGCCA 1 cut(s) 293
Mph1103I ATGCAT 1 cut(s) 1060
MscI TGGCCA 1 cut(s) 293
MseI TTAA 9 cut(s) 12, 935, 984, 1044, 1103, 1377, 1418, 1548, 1899
MslI CAYNNNNRTG 2 cut(s) 1173, 1284
Msp20I TGGCCA 1 cut(s) 293
MspCI CTTAAG 1 cut(s) 1898
MspI CCGG 7 cut(s) 248, 315, 449, 1077, 1113, 1571, 1652
MspR9I CCNGG 3 cut(s) 316, 1077, 1078
Mva1269I GAATGC 2 cut(s) 1291, 1608
MwoI GCNNNNNNNGC 2 cut(s) 56, 1363
NciI CCSGG 3 cut(s) 316, 1077, 1078
NcoI CCATGG 1 cut(s) 51
NdeI CATATG 2 cut(s) 620, 874
NdeII GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
NlaIII CATG 7 cut(s) 55, 594, 768, 1172, 1178, 1681, 1818
NlaIV GGNNCC 3 cut(s) 21, 1332, 1907
NmeAIII GCCGAG 1 cut(s) 194
NmuCI GTSAC 2 cut(s) 877, 1704
NsiI ATGCAT 1 cut(s) 1060
NspI RCATGY 1 cut(s) 1178
NspV TTCGAA 1 cut(s) 1771
PctI GAATGC 2 cut(s) 1291, 1608
PfeI GAWTC 4 cut(s) 244, 1121, 1409, 1535
PflFI GACNNNGTC 1 cut(s) 34
PkrI GCNGC 8 cut(s) 58, 76, 127, 1243, 1291, 1346, 1576, 1846
Ple19I CGATCG 1 cut(s) 1000
PleI GAGTC 3 cut(s) 25, 345, 1562
PpsI GAGTC 3 cut(s) 25, 345, 1562
PshBI ATTAAT 1 cut(s) 1044
Psp124BI GAGCTC 1 cut(s) 260
PspN4I GGNNCC 3 cut(s) 21, 1332, 1907
PspPI GGNCC 4 cut(s) 271, 611, 768, 1565
PsuI RGATCY 1 cut(s) 895
PsyI GACNNNGTC 1 cut(s) 34
PvuI CGATCG 1 cut(s) 1000
RsaI GTAC 3 cut(s) 268, 822, 1190
RsaNI GTAC 3 cut(s) 267, 821, 1189
RseI CAYNNNNRTG 2 cut(s) 1173, 1284
SacI GAGCTC 1 cut(s) 260
SaqAI TTAA 9 cut(s) 12, 935, 984, 1044, 1103, 1377, 1418, 1548, 1899
SatI GCNGC 8 cut(s) 57, 75, 126, 1242, 1290, 1345, 1575, 1845
Sau3AI GATC 8 cut(s) 319, 688, 895, 997, 1014, 1298, 1477, 1693
Sau96I GGNCC 4 cut(s) 271, 611, 768, 1565
SchI GAGTC 3 cut(s) 25, 346, 1563
ScrFI CCNGG 3 cut(s) 316, 1077, 1078
SduI GDGCHC 2 cut(s) 24, 260
SfaNI GCATC 5 cut(s) 578, 733, 1067, 1217, 1510
SfuI TTCGAA 1 cut(s) 1771
SgrAI CRCCGGYG 1 cut(s) 1112
SinI GGWCC 4 cut(s) 271, 611, 768, 1565
SmaI CCCGGG 1 cut(s) 1078
SmiMI CAYNNNNRTG 2 cut(s) 1173, 1284
SmlI CTYRAG 3 cut(s) 903, 1212, 1898
SmoI CTYRAG 3 cut(s) 903, 1212, 1898
SspI AATATT 1 cut(s) 1937
SspMI CTAG 2 cut(s) 477, 1589
SstI GAGCTC 1 cut(s) 260
StyD4I CCNGG 3 cut(s) 314, 1075, 1076
StyI CCWWGG 2 cut(s) 51, 1942
TaaI ACNGT 4 cut(s) 45, 266, 941, 1361
TaiI ACGT 1 cut(s) 681
TaqI TCGA 8 cut(s) 441, 961, 1297, 1612, 1689, 1761, 1771, 1782
TaqII GACCGA 1 cut(s) 1532
TatI WGTACW 2 cut(s) 820, 1188
TauI GCSGC 4 cut(s) 59, 128, 1244, 1347
TfiI GAWTC 4 cut(s) 244, 1121, 1409, 1535
Tru1I TTAA 9 cut(s) 12, 935, 984, 1044, 1103, 1377, 1418, 1548, 1899
Tru9I TTAA 9 cut(s) 12, 935, 984, 1044, 1103, 1377, 1418, 1548, 1899
TscAI CASTG 3 cut(s) 496, 517, 664
TseFI GTSAC 2 cut(s) 877, 1704
TseI GCWGC 4 cut(s) 74, 1289, 1574, 1844
Tsp45I GTSAC 2 cut(s) 877, 1704
TspDTI ATGAA 6 cut(s) 597, 612, 740, 861, 878, 1463
TspGWI ACGGA 2 cut(s) 284, 1841
TspMI CCCGGG 1 cut(s) 1076
TspRI CASTG 3 cut(s) 496, 517, 664
Tth111I GACNNNGTC 1 cut(s) 34
Vha464I CTTAAG 1 cut(s) 1898
VpaK11BI GGWCC 4 cut(s) 271, 611, 768, 1565
VspI ATTAAT 1 cut(s) 1044
XapI RAATTY 2 cut(s) 437, 1394
XceI RCATGY 1 cut(s) 1178
XmaI CCCGGG 1 cut(s) 1076
XmiI GTMKAC 1 cut(s) 404
XspI CTAG 2 cut(s) 477, 1589
Zsp2I ATGCAT 1 cut(s) 1060
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.