FvH4_3g43501

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0000117
Physical Location & Seq
Forward (+)
36240137 .. 36244456
4320 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g43501.t2

Sequence Viewer

Length: 1539 bp
ATGGCATTTCAAGGCACTCTCAACTTTGTAATCTTACTTGTTTTTAGCTATGGATTGGCAACTAGTATAGCTACCTCCCCTAGCCATTACGATGTTACTGCCATCAACCGGAGCACCTTTCCGGCAGGTTTCATATTCGGGACAGCTTCTTCGGCTTACCAGTACGAAGGTGCTGCAAAAGAAGATGGAAGAGGACCAAGCATATGGGACACATACACTCACACATTTCCAGATAAGATAAAGGACGGCAGCAATGGAGATGTCGCTATTGATGCATATCACCGCTACAAGGAGGATGTTGGCATCATGAAAGATATGGGTTTTGATGCTTACAGATTCTCCATCTCGTGGTCTAGATTGTTACCAAATGGAACGCTAAGTGGGGGTGTGAATAAGGAAGGAATCAAATACTATAACAACCTCATCAACGAGCTTCTAGCCAATGGTCTAAAACCCTTTGTCACCCTCTTCCATTGGGATCTTCCTCAAGCTTTAGAAGATGCATACGGTGGATTCTTAAGCCCTCAAATTGTAGATCATTTCCAAGACTACGCAGAGCTATGCTTTAAGGAATTTGGTGATAGAGTTAAGGACTGGATCACATTAAACGAGCCATGGAGCTACGCCCGTGGTGGTTATGTAATCGGGACGTTTGCACCGTGCCGATGTTCTGATTGGCAAAACCTGAATTGCACCGGTGGAGATTCAGGAACAGAGCCATATTTGGCCTCACACTACCAGCTTCTAGCTCACGCAGCCGCCGTAAAGTCATACAAAGAGAAGTATCAGGCTGAGCAGAAAGGTGTGATAGGGATAACAATACTTTCCCACTGGTTTGTGCCCTTTTCTGATGCCAAGCACGATAAAGAAGCTGCTCTACGAGCATTGGATTTTATGTTTGGATGGTATATGGATCCCTTAACATATGGTGAATATCCGCACAGCATGAGATCTCTTGTCGGAGATCGGTTACCGGAGTTCACGAAAGAGCAATCCGAGATGTTAAAAGGTTCATTTGATTTTCTAGGATTGAATTACTACACCGCTAACTATGCTACTTACGCACCTGAACTCAAGAATGCTGCAAACCCTAGCTACCTTACAGATGCTATCGCTATTCTTTCCACCGAGCGTAATGGTATTCCTATTGGTCCACAGTCTGCTTCAGATTGGCTCTATGTTTATCCAGAAGGAATTCGAGAACTTTTGCTTTACACGAAGGAAAAGTACAATGATCCACTTATTTACATCACTGAAAATGGAAGGGATGAGCACAACGATCCCAAATTAACACTTGAGGAAGCCCTAGCCGATACTCACCGAATCGACTTTTACTATCAACATCTTCATTATCTTCATGAAGCGATCAAGGATGGCGTTAACGTGAAGGGATATTTCGCATGGTCATTGTTTGACAACTTCGAATGGAATATGGGTTACAGTGTTAGGTTTGGCATCAACTATGTGGATTACAATGATGGCCTCAAGAGATACCCTAAACTGTCCGCACACTGGTTCAAGAATTTTCTTGGAAACTAG

Protein Analysis

513

Amino Acids

58.28

Weight (kDa)

5.27

Isoelectric Point (pI)

28.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 1 - 407 6.3e-130 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 116
AccB7I CCANNNNNTGG 1 cut(s) 348
AciI CCGC 5 cut(s) 283, 759, 938, 1044, 1506
AclWI GGATC 6 cut(s) 486, 605, 908, 921, 1229, 1274
AcsI RAATTY 3 cut(s) 572, 1194, 1522
AcuI CTGAAG 1 cut(s) 1149
AfaI GTAC 2 cut(s) 164, 1229
AfiI CCNNNNNNNGG 4 cut(s) 108, 289, 348, 1512
AflII CTTAAG 1 cut(s) 517
AgeI ACCGGT 1 cut(s) 695
AgsI TTSAA 3 cut(s) 11, 1033, 1519
AhlI ACTAGT 1 cut(s) 62
Alw21I GWGCWC 2 cut(s) 116, 1275
AlwI GGATC 6 cut(s) 486, 605, 908, 921, 1229, 1274
AoxI GGCC 2 cut(s) 726, 1480
ApeKI GCWGC 5 cut(s) 173, 249, 755, 872, 1082
ApoI RAATTY 3 cut(s) 572, 1194, 1522
ArsI GACNNNNNNTTYG 2 cut(s) 133, 165
AsiGI ACCGGT 1 cut(s) 695
Asp700I GAANNNNTTC 1 cut(s) 1194
AspS9I GGNCC 2 cut(s) 194, 1151
AsuHPI GGTGA 5 cut(s) 272, 454, 590, 941, 1310
AsuII TTCGAA 1 cut(s) 1422
AvaII GGWCC 2 cut(s) 194, 1151
BaeGI GKGCMC 1 cut(s) 843
BamHI GGATCC 1 cut(s) 913
BarI GAAGNNNNNNTAC 4 cut(s) 861, 893, 1211, 1243
BauI CACGAG 1 cut(s) 346
Bbv12I GWGCWC 2 cut(s) 116, 1275
BbvI GCAGC 5 cut(s) 160, 261, 767, 859, 1069
BccI CCATC 6 cut(s) 110, 179, 350, 897, 1367, 1472
BceAI ACGGC 2 cut(s) 262, 746
BcgI CGANNNNNNTGC 4 cut(s) 80, 114, 155, 189
BcuI ACTAGT 1 cut(s) 62
BfaI CTAG 9 cut(s) 63, 81, 354, 437, 746, 1025, 1092, 1307, 1537
BfrI CTTAAG 1 cut(s) 517
BfuAI ACCTGC 1 cut(s) 116
BglII AGATCT 1 cut(s) 950
BisI GCNGC 6 cut(s) 174, 250, 756, 759, 873, 1083
BlpI GCTNAGC 1 cut(s) 792
BlsI GCNGC 6 cut(s) 175, 251, 757, 760, 874, 1084
Bme18I GGWCC 2 cut(s) 194, 1151
BmgT120I GGNCC 2 cut(s) 194, 1151
BmiI GGNNCC 1 cut(s) 915
BmsI GCATC 7 cut(s) 262, 312, 316, 490, 841, 1096, 1464
Bpu1102I GCTNAGC 1 cut(s) 792
Bpu14I TTCGAA 1 cut(s) 1422
BpuEI CTTGAG 4 cut(s) 471, 1058, 1316, 1469
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 614, 628
BsaWI WCCGGW 3 cut(s) 108, 695, 973
BsaXI ACNNNNNCTCC 2 cut(s) 323, 353
Bsc4I CCNNNNNNNGG 4 cut(s) 108, 289, 348, 1512
Bse118I RCCGGY 1 cut(s) 695
Bse1I ACTGG 4 cut(s) 160, 599, 836, 1517
Bse3DI GCAATG 1 cut(s) 259
Bse8I GATNNNNATC 1 cut(s) 276
BseDI CCNNGG 2 cut(s) 614, 628
BseGI GGATG 4 cut(s) 301, 908, 1273, 1378
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 4 cut(s) 108, 289, 348, 1512
BseMI GCAATG 1 cut(s) 259
BseMII CTCAG 1 cut(s) 783
BseNI ACTGG 4 cut(s) 160, 599, 836, 1517
BseSI GKGCMC 1 cut(s) 843
BseXI GCAGC 5 cut(s) 160, 261, 767, 859, 1069
BshFI GGCC 2 cut(s) 728, 1482
BshTI ACCGGT 1 cut(s) 695
BsiHKAI GWGCWC 2 cut(s) 116, 1275
BsiSI CCGG 4 cut(s) 109, 122, 696, 974
BslFI GGGAC 3 cut(s) 154, 221, 661
BslI CCNNNNNNNGG 4 cut(s) 108, 289, 348, 1512
BsmFI GGGAC 3 cut(s) 154, 221, 661
BsmI GAATGC 1 cut(s) 1084
BsnI GGCC 2 cut(s) 728, 1482
Bsp119I TTCGAA 1 cut(s) 1422
Bsp1286I GDGCHC 3 cut(s) 116, 843, 1275
Bsp143I GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
Bsp1720I GCTNAGC 1 cut(s) 792
Bsp19I CCATGG 1 cut(s) 614
BspACI CCGC 5 cut(s) 283, 759, 938, 1044, 1506
BspANI GGCC 2 cut(s) 728, 1482
BspCNI CTCAG 1 cut(s) 784
BspHI TCATGA 2 cut(s) 306, 1357
BspLI GGNNCC 1 cut(s) 915
BspMI ACCTGC 1 cut(s) 116
BspPI GGATC 6 cut(s) 486, 605, 908, 921, 1229, 1274
BspT104I TTCGAA 1 cut(s) 1422
BspTI CTTAAG 1 cut(s) 517
BsrDI GCAATG 1 cut(s) 259
BsrFI RCCGGY 1 cut(s) 695
BsrI ACTGG 4 cut(s) 160, 599, 836, 1517
BssAI RCCGGY 1 cut(s) 695
BssECI CCNNGG 2 cut(s) 614, 628
BssMI GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
BssSI CACGAG 1 cut(s) 346
BssT1I CCWWGG 1 cut(s) 614
Bst2BI CACGAG 1 cut(s) 346
Bst4CI ACNGT 5 cut(s) 509, 660, 1158, 1442, 1503
Bst6I CTCTTC 2 cut(s) 184, 473
BstAFI CTTAAG 1 cut(s) 517
BstBI TTCGAA 1 cut(s) 1422
BstDEI CTNAG 2 cut(s) 377, 792
BstDSI CCRYGG 2 cut(s) 614, 628
BstEII GGTNACC 1 cut(s) 969
BstF5I GGATG 4 cut(s) 301, 908, 1273, 1378
BstKTI GATC 9 cut(s) 481, 538, 600, 916, 953, 967, 1237, 1282, 1368
BstMBI GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
BstMWI GCNNNNNNNGC 6 cut(s) 152, 272, 755, 881, 1052, 1061
BstPI GGTNACC 1 cut(s) 969
BstSLI GKGCMC 1 cut(s) 843
BstV1I GCAGC 5 cut(s) 160, 261, 767, 859, 1069
BstX2I RGATCY 3 cut(s) 478, 913, 950
BstXI CCANNNNNNTGG 1 cut(s) 204
BstYI RGATCY 3 cut(s) 478, 913, 950
BsuRI GGCC 2 cut(s) 728, 1482
BtgI CCRYGG 2 cut(s) 614, 628
BtsCI GGATG 4 cut(s) 301, 908, 1273, 1378
BtsIMutI CAGTG 4 cut(s) 829, 1251, 1447, 1510
BveI ACCTGC 1 cut(s) 116
CciI TCATGA 2 cut(s) 306, 1357
Cfr10I RCCGGY 1 cut(s) 695
Cfr13I GGNCC 2 cut(s) 194, 1151
Csp6I GTAC 2 cut(s) 163, 1228
CspAI ACCGGT 1 cut(s) 695
CviAII CATG 5 cut(s) 307, 615, 946, 1358, 1401
CviQI GTAC 2 cut(s) 163, 1228
DdeI CTNAG 2 cut(s) 377, 792
DpnI GATC 9 cut(s) 480, 537, 599, 915, 952, 966, 1236, 1281, 1367
DpnII GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
Eam1104I CTCTTC 2 cut(s) 184, 473
EarI CTCTTC 2 cut(s) 184, 473
Eco130I CCWWGG 1 cut(s) 614
Eco47I GGWCC 2 cut(s) 194, 1151
Eco57I CTGAAG 1 cut(s) 1149
Eco91I GGTNACC 1 cut(s) 969
EcoO65I GGTNACC 1 cut(s) 969
EcoRI GAATTC 1 cut(s) 1194
EcoT14I CCWWGG 1 cut(s) 614
EcoT22I ATGCAT 2 cut(s) 277, 505
ErhI CCWWGG 1 cut(s) 614
FaeI CATG 5 cut(s) 310, 618, 949, 1361, 1404
FaqI GGGAC 3 cut(s) 154, 221, 661
FatI CATG 5 cut(s) 306, 614, 945, 1357, 1400
FauNDI CATATG 2 cut(s) 203, 925
Fnu4HI GCNGC 6 cut(s) 174, 250, 756, 759, 873, 1083
FokI GGATG 4 cut(s) 308, 915, 1280, 1385
Fsp4HI GCNGC 6 cut(s) 174, 250, 756, 759, 873, 1083
FspBI CTAG 9 cut(s) 63, 81, 354, 437, 746, 1025, 1092, 1307, 1537
GluI GCNGC 6 cut(s) 174, 250, 756, 759, 873, 1083
HaeIII GGCC 2 cut(s) 728, 1482
HapII CCGG 4 cut(s) 109, 122, 696, 974
Hin1II CATG 5 cut(s) 310, 618, 949, 1361, 1404
HincII GTYRAC 1 cut(s) 1381
HindII GTYRAC 1 cut(s) 1381
HindIII AAGCTT 1 cut(s) 489
HinfI GANTC 5 cut(s) 336, 402, 513, 704, 1323
HpaI GTTAAC 1 cut(s) 1381
HpaII CCGG 4 cut(s) 109, 122, 696, 974
HphI GGTGA 5 cut(s) 272, 454, 590, 941, 1310
Hpy166II GTNNAC 3 cut(s) 981, 1154, 1381
Hpy188I TCNGA 5 cut(s) 673, 850, 962, 997, 1168
Hpy8I GTNNAC 3 cut(s) 981, 1154, 1381
HpyAV CCTTC 6 cut(s) 161, 392, 1184, 1213, 1257, 1381
HpyCH4III ACNGT 5 cut(s) 509, 660, 1158, 1442, 1503
HpyCH4IV ACGT 2 cut(s) 650, 1383
HpyCH4V TGCA 6 cut(s) 176, 275, 503, 656, 693, 1085
HpyF10VI GCNNNNNNNGC 6 cut(s) 152, 272, 755, 881, 1052, 1061
HpyF3I CTNAG 2 cut(s) 377, 792
HpySE526I ACGT 2 cut(s) 650, 1383
Hsp92II CATG 5 cut(s) 310, 618, 949, 1361, 1404
KspAI GTTAAC 1 cut(s) 1381
Kzo9I GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
LmnI GCTCC 2 cut(s) 111, 618
Lsp1109I GCAGC 5 cut(s) 160, 261, 767, 859, 1069
LweI GCATC 7 cut(s) 262, 312, 316, 490, 841, 1096, 1464
MaeI CTAG 9 cut(s) 63, 81, 354, 437, 746, 1025, 1092, 1307, 1537
MaeII ACGT 2 cut(s) 650, 1383
MaeIII GTNAC 5 cut(s) 94, 360, 460, 969, 1436
MalI GATC 9 cut(s) 480, 537, 599, 915, 952, 966, 1236, 1281, 1367
MboI GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
MboII GAAGA 8 cut(s) 141, 194, 201, 460, 473, 509, 1337, 1346
MflI RGATCY 3 cut(s) 478, 913, 950
MhlI GDGCHC 3 cut(s) 116, 843, 1275
MluCI AATT 7 cut(s) 528, 572, 688, 1033, 1194, 1286, 1522
MmeI TCCRAC 1 cut(s) 940
Mph1103I ATGCAT 2 cut(s) 277, 505
MroXI GAANNNNTTC 1 cut(s) 1194
MseI TTAA 8 cut(s) 518, 567, 588, 605, 920, 1004, 1289, 1380
MslI CAYNNNNRTG 1 cut(s) 90
MspCI CTTAAG 1 cut(s) 517
MspI CCGG 4 cut(s) 109, 122, 696, 974
Mva1269I GAATGC 1 cut(s) 1084
MwoI GCNNNNNNNGC 6 cut(s) 152, 272, 755, 881, 1052, 1061
NcoI CCATGG 1 cut(s) 614
NdeI CATATG 2 cut(s) 203, 925
NdeII GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
NlaIII CATG 5 cut(s) 310, 618, 949, 1361, 1404
NlaIV GGNNCC 1 cut(s) 915
NmuCI GTSAC 1 cut(s) 460
NsiI ATGCAT 2 cut(s) 277, 505
NspV TTCGAA 1 cut(s) 1422
PagI TCATGA 2 cut(s) 306, 1357
PcsI WCGNNNNNNNCGW 2 cut(s) 656, 759
PctI GAATGC 1 cut(s) 1084
PdmI GAANNNNTTC 1 cut(s) 1194
PfeI GAWTC 5 cut(s) 336, 402, 513, 704, 1323
PflMI CCANNNNNTGG 1 cut(s) 348
PinAI ACCGGT 1 cut(s) 695
PkrI GCNGC 6 cut(s) 175, 251, 757, 760, 874, 1084
PspEI GGTNACC 1 cut(s) 969
PspN4I GGNNCC 1 cut(s) 915
PspPI GGNCC 2 cut(s) 194, 1151
PsuI RGATCY 3 cut(s) 478, 913, 950
RsaI GTAC 2 cut(s) 164, 1229
RsaNI GTAC 2 cut(s) 163, 1228
RseI CAYNNNNRTG 1 cut(s) 90
SaqAI TTAA 8 cut(s) 518, 567, 588, 605, 920, 1004, 1289, 1380
SatI GCNGC 6 cut(s) 174, 250, 756, 759, 873, 1083
Sau3AI GATC 9 cut(s) 478, 535, 597, 913, 950, 964, 1234, 1279, 1365
Sau96I GGNCC 2 cut(s) 194, 1151
SduI GDGCHC 3 cut(s) 116, 843, 1275
SfaNI GCATC 7 cut(s) 262, 312, 316, 490, 841, 1096, 1464
SfuI TTCGAA 1 cut(s) 1422
SgrAI CRCCGGYG 1 cut(s) 695
SinI GGWCC 2 cut(s) 194, 1151
SmiMI CAYNNNNRTG 1 cut(s) 90
SmlI CTYRAG 5 cut(s) 486, 517, 1073, 1295, 1484
SmoI CTYRAG 5 cut(s) 486, 517, 1073, 1295, 1484
SpeI ACTAGT 1 cut(s) 62
Sse9I AATT 7 cut(s) 528, 572, 688, 1033, 1194, 1286, 1522
SsiI CCGC 5 cut(s) 283, 759, 938, 1044, 1506
SspMI CTAG 9 cut(s) 63, 81, 354, 437, 746, 1025, 1092, 1307, 1537
StyI CCWWGG 1 cut(s) 614
TaaI ACNGT 5 cut(s) 509, 660, 1158, 1442, 1503
TaiI ACGT 2 cut(s) 653, 1386
TaqI TCGA 3 cut(s) 1198, 1326, 1422
TasI AATT 7 cut(s) 528, 572, 688, 1033, 1194, 1286, 1522
TatI WGTACW 1 cut(s) 1227
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 5 cut(s) 336, 402, 513, 704, 1323
Tru1I TTAA 8 cut(s) 518, 567, 588, 605, 920, 1004, 1289, 1380
Tru9I TTAA 8 cut(s) 518, 567, 588, 605, 920, 1004, 1289, 1380
TscAI CASTG 4 cut(s) 836, 1258, 1447, 1517
TseFI GTSAC 1 cut(s) 460
TseI GCWGC 5 cut(s) 173, 249, 755, 872, 1082
Tsp45I GTSAC 1 cut(s) 460
TspDTI ATGAA 6 cut(s) 121, 323, 1002, 1337, 1346, 1374
TspRI CASTG 4 cut(s) 836, 1258, 1447, 1517
Van91I CCANNNNNTGG 1 cut(s) 348
Vha464I CTTAAG 1 cut(s) 517
VpaK11BI GGWCC 2 cut(s) 194, 1151
XapI RAATTY 3 cut(s) 572, 1194, 1522
XbaI TCTAGA 1 cut(s) 353
XmnI GAANNNNTTC 1 cut(s) 1194
XspI CTAG 9 cut(s) 63, 81, 354, 437, 746, 1025, 1092, 1307, 1537
Zsp2I ATGCAT 2 cut(s) 277, 505
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.