Belongs to the glycosyl hydrolase 1 family
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| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| Acc36I | ACCTGC | 2 cut(s) | 716, 1111 |
| Acc65I | GGTACC | 1 cut(s) | 1503 |
| AccB1I | GGYRCC | 1 cut(s) | 1503 |
| AclWI | GGATC | 7 cut(s) | 169, 623, 926, 939, 1115, 1244, 1322 |
| AcoI | YGGCCR | 1 cut(s) | 1572 |
| AcsI | RAATTY | 5 cut(s) | 800, 995, 1209, 1513, 1534 |
| AcuI | CTGAAG | 1 cut(s) | 1164 |
| AfaI | GTAC | 5 cut(s) | 671, 791, 959, 1083, 1505 |
| AflIII | ACRYGT | 1 cut(s) | 1126 |
| AgsI | TTSAA | 6 cut(s) | 425, 625, 1051, 1291, 1439, 1563 |
| AjnI | CCWGG | 2 cut(s) | 142, 177 |
| AluBI | AGCT | 4 cut(s) | 77, 173, 776, 902 |
| AluI | AGCT | 4 cut(s) | 77, 173, 776, 902 |
| Alw26I | GTCTC | 1 cut(s) | 1581 |
| AlwI | GGATC | 7 cut(s) | 169, 623, 926, 939, 1115, 1244, 1322 |
| AlwNI | CAGNNNCTG | 1 cut(s) | 779 |
| AoxI | GGCC | 3 cut(s) | 368, 970, 1572 |
| ApeKI | GCWGC | 7 cut(s) | 77, 170, 773, 776, 890, 1175, 1376 |
| ApoI | RAATTY | 5 cut(s) | 800, 995, 1209, 1513, 1534 |
| Asp700I | GAANNNNTTC | 1 cut(s) | 453 |
| Asp718I | GGTACC | 1 cut(s) | 1503 |
| AspLEI | GCGC | 1 cut(s) | 1551 |
| AspS9I | GGNCC | 4 cut(s) | 120, 212, 369, 1166 |
| AsuC2I | CCSGG | 1 cut(s) | 678 |
| AsuHPI | GGTGA | 4 cut(s) | 226, 608, 835, 1259 |
| AvaII | GGWCC | 3 cut(s) | 120, 212, 1166 |
| AxyI | CCTNAGG | 1 cut(s) | 1170 |
| BaeI | ACNNNNGTAYC | 2 cut(s) | 1065, 1098 |
| BalI | TGGCCA | 1 cut(s) | 1574 |
| BamHI | GGATCC | 1 cut(s) | 931 |
| BanI | GGYRCC | 1 cut(s) | 1503 |
| BbvI | GCAGC | 7 cut(s) | 64, 182, 763, 785, 877, 1162, 1388 |
| BccI | CCATC | 5 cut(s) | 197, 252, 647, 915, 1145 |
| BciT130I | CCWGG | 2 cut(s) | 144, 179 |
| BciVI | GTATCC | 1 cut(s) | 1400 |
| BclI | TGATCA | 2 cut(s) | 262, 289 |
| BcnI | CCSGG | 1 cut(s) | 678 |
| BcoDI | GTCTC | 1 cut(s) | 1581 |
| BfaI | CTAG | 1 cut(s) | 1310 |
| BfmI | CTRYAG | 1 cut(s) | 165 |
| BfuAI | ACCTGC | 2 cut(s) | 716, 1111 |
| BfuI | GTATCC | 1 cut(s) | 1400 |
| BglII | AGATCT | 1 cut(s) | 277 |
| BisI | GCNGC | 7 cut(s) | 78, 171, 774, 777, 891, 1176, 1377 |
| BlpI | GCTNAGC | 1 cut(s) | 81 |
| BlsI | GCNGC | 7 cut(s) | 79, 172, 775, 778, 892, 1177, 1378 |
| Bme1390I | CCNGG | 3 cut(s) | 144, 179, 678 |
| Bme18I | GGWCC | 3 cut(s) | 120, 212, 1166 |
| BmgT120I | GGNCC | 4 cut(s) | 120, 212, 369, 1166 |
| BmiI | GGNNCC | 2 cut(s) | 933, 1505 |
| BmrFI | CCNGG | 3 cut(s) | 144, 179, 678 |
| BmrI | ACTGGG | 2 cut(s) | 502, 1035 |
| BmsI | GCATC | 7 cut(s) | 121, 334, 508, 674, 859, 877, 1306 |
| BmuI | ACTGGG | 2 cut(s) | 502, 1035 |
| BplI | GAGNNNNNCTC | 2 cut(s) | 848, 880 |
| Bpu1102I | GCTNAGC | 1 cut(s) | 81 |
| BpuEI | CTTGAG | 1 cut(s) | 1526 |
| BpuMI | CCSGG | 1 cut(s) | 678 |
| Bsa29I | ATCGAT | 1 cut(s) | 258 |
| BsaJI | CCNNGG | 3 cut(s) | 143, 649, 712 |
| Bse1I | ACTGG | 4 cut(s) | 497, 617, 1030, 1190 |
| Bse21I | CCTNAGG | 1 cut(s) | 1170 |
| Bse3DI | GCAATG | 3 cut(s) | 12, 277, 466 |
| BseBI | CCWGG | 2 cut(s) | 144, 179 |
| BseCI | ATCGAT | 1 cut(s) | 258 |
| BseDI | CCNNGG | 3 cut(s) | 143, 649, 712 |
| BseGI | GGATG | 5 cut(s) | 244, 349, 502, 672, 926 |
| BseMI | GCAATG | 3 cut(s) | 12, 277, 466 |
| BseMII | CTCAG | 3 cut(s) | 879, 1184, 1260 |
| BseNI | ACTGG | 4 cut(s) | 497, 617, 1030, 1190 |
| BseXI | GCAGC | 7 cut(s) | 64, 182, 763, 785, 877, 1162, 1388 |
| BshFI | GGCC | 3 cut(s) | 370, 972, 1574 |
| BshNI | GGYRCC | 1 cut(s) | 1503 |
| BshVI | ATCGAT | 1 cut(s) | 258 |
| BsiSI | CCGG | 1 cut(s) | 677 |
| BslFI | GGGAC | 2 cut(s) | 239, 693 |
| BsmAI | GTCTC | 1 cut(s) | 1581 |
| BsmFI | GGGAC | 2 cut(s) | 239, 693 |
| BsmI | GAATGC | 1 cut(s) | 1155 |
| BsnI | GGCC | 3 cut(s) | 370, 972, 1574 |
| Bsp1407I | TGTACA | 1 cut(s) | 789 |
| Bsp1720I | GCTNAGC | 1 cut(s) | 81 |
| Bsp19I | CCATGG | 1 cut(s) | 649 |
| BspANI | GGCC | 3 cut(s) | 370, 972, 1574 |
| BspCNI | CTCAG | 3 cut(s) | 878, 1183, 1261 |
| BspDI | ATCGAT | 1 cut(s) | 258 |
| BspLI | GGNNCC | 2 cut(s) | 933, 1505 |
| BspMI | ACCTGC | 2 cut(s) | 716, 1111 |
| BspPI | GGATC | 7 cut(s) | 169, 623, 926, 939, 1115, 1244, 1322 |
| BspT107I | GGYRCC | 1 cut(s) | 1503 |
| BsrDI | GCAATG | 3 cut(s) | 12, 277, 466 |
| BsrGI | TGTACA | 1 cut(s) | 789 |
| BsrI | ACTGG | 4 cut(s) | 497, 617, 1030, 1190 |
| BssECI | CCNNGG | 3 cut(s) | 143, 649, 712 |
| BssT1I | CCWWGG | 2 cut(s) | 649, 712 |
| Bst2UI | CCWGG | 2 cut(s) | 144, 179 |
| Bst4CI | ACNGT | 1 cut(s) | 1459 |
| BstAUI | TGTACA | 1 cut(s) | 789 |
| BstC8I | GCNNGC | 2 cut(s) | 392, 861 |
| BstDEI | CTNAG | 6 cut(s) | 81, 395, 865, 1086, 1170, 1269 |
| BstDSI | CCRYGG | 1 cut(s) | 649 |
| BstF5I | GGATG | 5 cut(s) | 244, 349, 502, 672, 926 |
| BstHHI | GCGC | 1 cut(s) | 1551 |
| BstMAI | GTCTC | 1 cut(s) | 1581 |
| BstMWI | GCNNNNNNNGC | 4 cut(s) | 773, 869, 899, 1070 |
| BstNI | CCWGG | 2 cut(s) | 144, 179 |
| BstNSI | RCATGY | 1 cut(s) | 1130 |
| BstSCI | CCNGG | 3 cut(s) | 142, 177, 676 |
| BstSFI | CTRYAG | 1 cut(s) | 165 |
| BstV1I | GCAGC | 7 cut(s) | 64, 182, 763, 785, 877, 1162, 1388 |
| BstX2I | RGATCY | 3 cut(s) | 161, 277, 931 |
| BstXI | CCANNNNNNTGG | 1 cut(s) | 222 |
| BstYI | RGATCY | 3 cut(s) | 161, 277, 931 |
| Bsu15I | ATCGAT | 1 cut(s) | 258 |
| Bsu36I | CCTNAGG | 1 cut(s) | 1170 |
| BsuI | GTATCC | 1 cut(s) | 1400 |
| BsuRI | GGCC | 3 cut(s) | 370, 972, 1574 |
| BsuTUI | ATCGAT | 1 cut(s) | 258 |
| BtgI | CCRYGG | 1 cut(s) | 649 |
| BtsCI | GGATG | 5 cut(s) | 244, 349, 502, 672, 926 |
| BtsIMutI | CAGTG | 4 cut(s) | 490, 610, 1136, 1590 |
| BveI | ACCTGC | 2 cut(s) | 716, 1111 |
| Cac8I | GCNNGC | 2 cut(s) | 392, 861 |
| CaiI | CAGNNNCTG | 1 cut(s) | 779 |
| CfoI | GCGC | 1 cut(s) | 1551 |
| Cfr13I | GGNCC | 4 cut(s) | 120, 212, 369, 1166 |
| ClaI | ATCGAT | 1 cut(s) | 258 |
| CsiI | ACCWGGT | 1 cut(s) | 177 |
| Csp6I | GTAC | 5 cut(s) | 670, 790, 958, 1082, 1504 |
| CviQI | GTAC | 5 cut(s) | 670, 790, 958, 1082, 1504 |
| DdeI | CTNAG | 6 cut(s) | 81, 395, 865, 1086, 1170, 1269 |
| DraI | TTTAAA | 1 cut(s) | 562 |
| EaeI | YGGCCR | 1 cut(s) | 1572 |
| Eco130I | CCWWGG | 2 cut(s) | 649, 712 |
| Eco147I | AGGCCT | 1 cut(s) | 972 |
| Eco47I | GGWCC | 3 cut(s) | 120, 212, 1166 |
| Eco57I | CTGAAG | 1 cut(s) | 1164 |
| Eco81I | CCTNAGG | 1 cut(s) | 1170 |
| EcoRI | GAATTC | 1 cut(s) | 1209 |
| EcoRII | CCWGG | 2 cut(s) | 142, 177 |
| EcoT14I | CCWWGG | 2 cut(s) | 649, 712 |
| EcoT22I | ATGCAT | 2 cut(s) | 12, 523 |
| ErhI | CCWWGG | 2 cut(s) | 649, 712 |
| FaqI | GGGAC | 2 cut(s) | 239, 693 |
| FauNDI | CATATG | 2 cut(s) | 221, 523 |
| FbaI | TGATCA | 2 cut(s) | 262, 289 |
| Fnu4HI | GCNGC | 7 cut(s) | 78, 171, 774, 777, 891, 1176, 1377 |
| FokI | GGATG | 5 cut(s) | 231, 356, 509, 679, 933 |
| Fsp4HI | GCNGC | 7 cut(s) | 78, 171, 774, 777, 891, 1176, 1377 |
| FspBI | CTAG | 1 cut(s) | 1310 |
| GlaI | GCGC | 1 cut(s) | 1550 |
| GluI | GCNGC | 7 cut(s) | 78, 171, 774, 777, 891, 1176, 1377 |
| HaeIII | GGCC | 3 cut(s) | 370, 972, 1574 |
| HapII | CCGG | 1 cut(s) | 677 |
| HhaI | GCGC | 1 cut(s) | 1551 |
| Hin6I | GCGC | 1 cut(s) | 1549 |
| HinP1I | GCGC | 1 cut(s) | 1549 |
| HincII | GTYRAC | 2 cut(s) | 409, 1023 |
| HindII | GTYRAC | 2 cut(s) | 409, 1023 |
| HinfI | GANTC | 4 cut(s) | 722, 1010, 1295, 1494 |
| HpaI | GTTAAC | 1 cut(s) | 1023 |
| HpaII | CCGG | 1 cut(s) | 677 |
| HphI | GGTGA | 4 cut(s) | 226, 608, 835, 1259 |
| Hpy166II | GTNNAC | 5 cut(s) | 409, 790, 960, 1023, 1530 |
| Hpy188I | TCNGA | 3 cut(s) | 868, 1183, 1203 |
| Hpy188III | TCNNGA | 5 cut(s) | 248, 664, 1214, 1384, 1543 |
| Hpy8I | GTNNAC | 5 cut(s) | 409, 790, 960, 1023, 1530 |
| HpyAV | CCTTC | 6 cut(s) | 179, 241, 322, 409, 1381, 1442 |
| HpyCH4III | ACNGT | 1 cut(s) | 1459 |
| HpyCH4IV | ACGT | 3 cut(s) | 198, 1147, 1368 |
| HpyF10VI | GCNNNNNNNGC | 4 cut(s) | 773, 869, 899, 1070 |
| HpyF3I | CTNAG | 6 cut(s) | 81, 395, 865, 1086, 1170, 1269 |
| HpySE526I | ACGT | 3 cut(s) | 198, 1147, 1368 |
| HspAI | GCGC | 1 cut(s) | 1549 |
| KpnI | GGTACC | 1 cut(s) | 1507 |
| Ksp22I | TGATCA | 2 cut(s) | 262, 289 |
| KspAI | GTTAAC | 1 cut(s) | 1023 |
| Lsp1109I | GCAGC | 7 cut(s) | 64, 182, 763, 785, 877, 1162, 1388 |
| LweI | GCATC | 7 cut(s) | 121, 334, 508, 674, 859, 877, 1306 |
| MabI | ACCWGGT | 1 cut(s) | 177 |
| MaeI | CTAG | 1 cut(s) | 1310 |
| MaeII | ACGT | 3 cut(s) | 198, 1147, 1368 |
| MaeIII | GTNAC | 8 cut(s) | 104, 378, 478, 644, 745, 843, 1109, 1364 |
| MboII | GAAGA | 5 cut(s) | 527, 890, 1016, 1019, 1246 |
| MflI | RGATCY | 3 cut(s) | 161, 277, 931 |
| MlsI | TGGCCA | 1 cut(s) | 1574 |
| MluNI | TGGCCA | 1 cut(s) | 1574 |
| MlyI | GAGTC | 1 cut(s) | 1488 |
| MmeI | TCCRAC | 4 cut(s) | 321, 1024, 1323, 1564 |
| MnlI | CCTC | 9 cut(s) | 203, 311, 552, 874, 938, 962, 1179, 1331, 1550 |
| Mox20I | TGGCCA | 1 cut(s) | 1574 |
| Mph1103I | ATGCAT | 2 cut(s) | 12, 523 |
| MroXI | GAANNNNTTC | 1 cut(s) | 453 |
| MscI | TGGCCA | 1 cut(s) | 1574 |
| MseI | TTAA | 5 cut(s) | 536, 561, 978, 1022, 1473 |
| MslI | CAYNNNNRTG | 2 cut(s) | 270, 495 |
| Msp20I | TGGCCA | 1 cut(s) | 1574 |
| MspA1I | CMGCKG | 1 cut(s) | 776 |
| MspI | CCGG | 1 cut(s) | 677 |
| MspR9I | CCNGG | 3 cut(s) | 144, 179, 678 |
| Mva1269I | GAATGC | 1 cut(s) | 1155 |
| MvaI | CCWGG | 2 cut(s) | 144, 179 |
| MwoI | GCNNNNNNNGC | 4 cut(s) | 773, 869, 899, 1070 |
| NciI | CCSGG | 1 cut(s) | 678 |
| NcoI | CCATGG | 1 cut(s) | 649 |
| NdeI | CATATG | 2 cut(s) | 221, 523 |
| NlaIV | GGNNCC | 2 cut(s) | 933, 1505 |
| NmuCI | GTSAC | 2 cut(s) | 478, 843 |
| NsiI | ATGCAT | 2 cut(s) | 12, 523 |
| NspI | RCATGY | 1 cut(s) | 1130 |
| PceI | AGGCCT | 1 cut(s) | 972 |
| PciI | ACATGT | 1 cut(s) | 1126 |
| PctI | GAATGC | 1 cut(s) | 1155 |
| PdmI | GAANNNNTTC | 1 cut(s) | 453 |
| PfeI | GAWTC | 3 cut(s) | 722, 1010, 1295 |
| PkrI | GCNGC | 7 cut(s) | 79, 172, 775, 778, 892, 1177, 1378 |
| PleI | GAGTC | 1 cut(s) | 1488 |
| PpsI | GAGTC | 1 cut(s) | 1488 |
| PscI | ACATGT | 1 cut(s) | 1126 |
| Psp6I | CCWGG | 2 cut(s) | 142, 177 |
| PspGI | CCWGG | 2 cut(s) | 142, 177 |
| PspN4I | GGNNCC | 2 cut(s) | 933, 1505 |
| PspPI | GGNCC | 4 cut(s) | 120, 212, 369, 1166 |
| PstNI | CAGNNNCTG | 1 cut(s) | 779 |
| PsuI | RGATCY | 3 cut(s) | 161, 277, 931 |
| PvuII | CAGCTG | 1 cut(s) | 776 |
| RsaI | GTAC | 5 cut(s) | 671, 791, 959, 1083, 1505 |
| RsaNI | GTAC | 5 cut(s) | 670, 790, 958, 1082, 1504 |
| RseI | CAYNNNNRTG | 2 cut(s) | 270, 495 |
| SaqAI | TTAA | 5 cut(s) | 536, 561, 978, 1022, 1473 |
| SatI | GCNGC | 7 cut(s) | 78, 171, 774, 777, 891, 1176, 1377 |
| Sau96I | GGNCC | 4 cut(s) | 120, 212, 369, 1166 |
| SchI | GAGTC | 1 cut(s) | 1488 |
| ScrFI | CCNGG | 3 cut(s) | 144, 179, 678 |
| SexAI | ACCWGGT | 1 cut(s) | 177 |
| SfaNI | GCATC | 7 cut(s) | 121, 334, 508, 674, 859, 877, 1306 |
| SfcI | CTRYAG | 1 cut(s) | 165 |
| SinI | GGWCC | 3 cut(s) | 120, 212, 1166 |
| SmiMI | CAYNNNNRTG | 2 cut(s) | 270, 495 |
| SmlI | CTYRAG | 1 cut(s) | 1541 |
| SmoI | CTYRAG | 1 cut(s) | 1541 |
| SseBI | AGGCCT | 1 cut(s) | 972 |
| SspI | AATATT | 1 cut(s) | 1567 |
| SspMI | CTAG | 1 cut(s) | 1310 |
| StuI | AGGCCT | 1 cut(s) | 972 |
| StyD4I | CCNGG | 3 cut(s) | 142, 177, 676 |
| StyI | CCWWGG | 2 cut(s) | 649, 712 |
| TaaI | ACNGT | 1 cut(s) | 1459 |
| TaiI | ACGT | 3 cut(s) | 201, 1150, 1371 |
| TaqI | TCGA | 6 cut(s) | 258, 552, 601, 898, 1462, 1517 |
| TatI | WGTACW | 1 cut(s) | 789 |
| TfiI | GAWTC | 3 cut(s) | 722, 1010, 1295 |
| Tru1I | TTAA | 5 cut(s) | 536, 561, 978, 1022, 1473 |
| Tru9I | TTAA | 5 cut(s) | 536, 561, 978, 1022, 1473 |
| TscAI | CASTG | 4 cut(s) | 497, 617, 1143, 1597 |
| TseFI | GTSAC | 2 cut(s) | 478, 843 |
| TseI | GCWGC | 7 cut(s) | 77, 170, 773, 776, 890, 1175, 1376 |
| Tsp45I | GTSAC | 2 cut(s) | 478, 843 |
| TspDTI | ATGAA | 5 cut(s) | 341, 350, 462, 642, 1361 |
| TspGWI | ACGGA | 2 cut(s) | 1016, 1506 |
| TspRI | CASTG | 4 cut(s) | 497, 617, 1143, 1597 |
| VpaK11BI | GGWCC | 3 cut(s) | 120, 212, 1166 |
| XapI | RAATTY | 5 cut(s) | 800, 995, 1209, 1513, 1534 |
| XceI | RCATGY | 1 cut(s) | 1130 |
| XmnI | GAANNNNTTC | 1 cut(s) | 453 |
| XspI | CTAG | 1 cut(s) | 1310 |
| Zsp2I | ATGCAT | 2 cut(s) | 12, 523 |