Rw5G047480

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0000117
Physical Location & Seq
Forward (+)
82109349 .. 82115593
6245 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G047480.1

Sequence Viewer

Length: 1551 bp
ATGGCAATGCAAGGATCTCTGTTTCTAGGCGTAGTGCTTCTACTTGTTGCAGGCTTTAGATTGACAAGCAGTGAAGCTCTTACACCCAGTACTCCTATCTATGACACAGGTTTCCTCAACAGGACTAGCTTCCCAGCAGGTTTCATATTTGGGACAGCTTCAGCGTCATATCAGTATGAAGGTGCTGCTAAAGAAGGTGGTAGAGGACCGAGCATATGGGATACCTACACCCACGAACATTCAGAAAGGATCACGGGTGGAAGCAATGGAGATGTGGCTAATGATCAATATCACCATTATAAGGAAGATGTGGGGATTATGAAGAATATGAATTTGGATGCTTATAGGTTCTCTATCTCATGGTCAAGATTGTTACCAAATGGAAAGCTAAGTGGGGGAGTGAACAAGGAAGGAGTCAAGTACTACAACAATCTCATCAATGAACTCCTAAGCAATGGTCTAAAGCCATTTGTGACCCTTTTTCATTGGGATCTTCCCCAAACTTTAGAAGACGAATATGGCGGTTTCTTAAGCCCTAACGTTGTCAAGCATTTTCGGGACTATGCGGAGCTGTGTTACAAGGAATTTGGTGATCGGGTAAAGCACTGGATCACGTTGAATGAGCCATGGTCATACAGTAATGGTGGTTATGCAGTCGGGTCATTGGCACCAGGACGGTGTTCTGCGTGGCAGCAGCTAAATTGCACCGGCGGGGATTCGGGTACTGAACCATACTTGGTGGCACACCACTTACTCCTTTCTCATGCAGCTGCTGTAAAGTTGTACAAGCAGAAATATCAGGCATCTCAGAAAGGAGTTATAGGAGTGACCCTAGTGTCTCACTGGTTCATTCCAATTTCAGGGGCAAAGCACAACAAAAATGCTGCCTTACGATCTTTGGATTTTATGTTTGGATGGTTCATGGATCCTTTGACAAACGGAGACTATCCACACAGCATGCGATCTCTCGTTGGAAACCGATTACCCAAATTCACAAAAGAACAATCCAAGTTACTAATCGGATCATTTGATTTTCTTGGACTAAATTACTATACTACTTACTATGCAACATATACACCTCAGAACAACTCTTTAAATGCAAGCTACTTGACAGACGCTCGTGCTACTCAATCATTTGCGCTAAATGGCGTCCCCATTGGTCCACAGACTGCTTCATCATGGCTATATGTTTATCCAAAAGGAATTAGAGACCTTTTACTCTACACAAGGACAAAGTATAATGATCCACTTATTTACATTACTGAGAATGGCATTGATGAGTTCAATGATCCCAAATTATCACTTGAGGAAGCTCTCTATGACACTCAGAGAGTTGACTATTACTTCCGCCACCTCTATTACCTTCAAAAAGCAATCAAGGAAGGTGTGAACTTGAAGGGCTACTTTGCATGGTCATTGCTAGACAACTTCGAATGGGGTTCAGGTTACACAGTTCGATTTGGTCTCAACTATGTGGATTACAAAAATGGAAACAAAAGACACCCAAAACTCTCGGCACATTGGTTCAAAAACTTCCTCAAGATGTATTGA

Protein Analysis

516

Amino Acids

58.52

Weight (kDa)

8.54

Isoelectric Point (pI)

23.72

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 42 - 514 9.1e-157 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 300
AasI GACNNNNNNGTC 1 cut(s) 835
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 667
AciI CCGC 4 cut(s) 522, 566, 711, 1348
AclI AACGTT 1 cut(s) 540
AclWI GGATC 9 cut(s) 22, 257, 498, 617, 920, 933, 1030, 1238, 1283
AcsI RAATTY 3 cut(s) 331, 584, 989
AcuI CTGAAG 1 cut(s) 144
AcyI GRCGYC 1 cut(s) 1149
AfaI GTAC 4 cut(s) 91, 422, 724, 785
AfiI CCNNNNNNNGG 2 cut(s) 301, 860
AflII CTTAAG 1 cut(s) 529
AgsI TTSAA 5 cut(s) 619, 1285, 1367, 1396, 1528
AjnI CCWGG 1 cut(s) 670
AjuI GAANNNNNNNTTGG 2 cut(s) 317, 349
AluBI AGCT 9 cut(s) 77, 129, 158, 388, 571, 697, 770, 1104, 1313
AluI AGCT 9 cut(s) 77, 129, 158, 388, 571, 697, 770, 1104, 1313
Alw26I GTCTC 4 cut(s) 843, 936, 1203, 1469
AlwI GGATC 9 cut(s) 22, 257, 498, 617, 920, 933, 1030, 1238, 1283
AlwNI CAGNNNCTG 1 cut(s) 773
ApeKI GCWGC 6 cut(s) 185, 691, 694, 767, 770, 884
ApoI RAATTY 3 cut(s) 331, 584, 989
AspLEI GCGC 1 cut(s) 1141
AspS9I GGNCC 2 cut(s) 206, 1160
AsuHPI GGTGA 2 cut(s) 284, 602
AsuII TTCGAA 1 cut(s) 1431
AvaII GGWCC 2 cut(s) 206, 1160
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 1 cut(s) 667
BauI CACGAG 1 cut(s) 1119
BbsI GAAGAC 1 cut(s) 516
BbvI GCAGC 6 cut(s) 172, 703, 706, 757, 779, 871
BccI CCATC 1 cut(s) 909
BciT130I CCWGG 1 cut(s) 672
BciVI GTATCC 1 cut(s) 214
BclI TGATCA 1 cut(s) 283
BcoDI GTCTC 4 cut(s) 843, 936, 1203, 1469
BfaI CTAG 4 cut(s) 26, 126, 833, 1421
BfrI CTTAAG 1 cut(s) 529
BfuAI ACCTGC 1 cut(s) 128
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 6 cut(s) 186, 692, 695, 768, 771, 885
BlsI GCNGC 6 cut(s) 187, 693, 696, 769, 772, 886
BmcAI AGTACT 2 cut(s) 91, 422
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 2 cut(s) 206, 1160
BmgT120I GGNCC 2 cut(s) 206, 1160
BmiI GGNNCC 2 cut(s) 669, 927
BmrFI CCNGG 1 cut(s) 672
BmrI ACTGGG 1 cut(s) 81
BmsI GCATC 2 cut(s) 328, 812
BmuI ACTGGG 1 cut(s) 81
BpiI GAAGAC 1 cut(s) 516
Bpu10I CCTNAGC 1 cut(s) 449
Bpu14I TTCGAA 1 cut(s) 1431
BpuEI CTTGAG 2 cut(s) 1325, 1523
BsaBI GATNNNNATC 1 cut(s) 288
BsaHI GRCGYC 1 cut(s) 1149
BsaI GGTCTC 2 cut(s) 1203, 1469
BsaJI CCNNGG 1 cut(s) 626
Bsc4I CCNNNNNNNGG 2 cut(s) 301, 860
Bse118I RCCGGY 1 cut(s) 707
Bse1I ACTGG 3 cut(s) 87, 611, 848
Bse3DI GCAATG 4 cut(s) 12, 271, 460, 1415
Bse8I GATNNNNATC 1 cut(s) 288
BseBI CCWGG 1 cut(s) 672
BseDI CCNNGG 1 cut(s) 626
BseGI GGATG 2 cut(s) 343, 920
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 2 cut(s) 301, 860
BseMI GCAATG 4 cut(s) 12, 271, 460, 1415
BseMII CTCAG 4 cut(s) 821, 1094, 1254, 1340
BseNI ACTGG 3 cut(s) 87, 611, 848
BseXI GCAGC 6 cut(s) 172, 703, 706, 757, 779, 871
BseYI CCCAGC 1 cut(s) 133
BshNI GGYRCC 1 cut(s) 667
BsiSI CCGG 1 cut(s) 708
BslFI GGGAC 3 cut(s) 166, 572, 1136
BslI CCNNNNNNNGG 2 cut(s) 301, 860
BsmAI GTCTC 4 cut(s) 843, 936, 1203, 1469
BsmFI GGGAC 3 cut(s) 166, 572, 1136
Bso31I GGTCTC 2 cut(s) 1203, 1469
Bsp119I TTCGAA 1 cut(s) 1431
Bsp1407I TGTACA 1 cut(s) 783
Bsp19I CCATGG 1 cut(s) 626
BspACI CCGC 4 cut(s) 522, 566, 711, 1348
BspCNI CTCAG 4 cut(s) 820, 1093, 1255, 1339
BspLI GGNNCC 2 cut(s) 669, 927
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 9 cut(s) 22, 257, 498, 617, 920, 933, 1030, 1238, 1283
BspT104I TTCGAA 1 cut(s) 1431
BspT107I GGYRCC 1 cut(s) 667
BspTI CTTAAG 1 cut(s) 529
BspTNI GGTCTC 2 cut(s) 1203, 1469
BsrDI GCAATG 4 cut(s) 12, 271, 460, 1415
BsrFI RCCGGY 1 cut(s) 707
BsrGI TGTACA 1 cut(s) 783
BsrI ACTGG 3 cut(s) 87, 611, 848
BssAI RCCGGY 1 cut(s) 707
BssECI CCNNGG 1 cut(s) 626
BssNI GRCGYC 1 cut(s) 1149
BssSI CACGAG 1 cut(s) 1119
BssT1I CCWWGG 1 cut(s) 626
Bst2BI CACGAG 1 cut(s) 1119
Bst2UI CCWGG 1 cut(s) 672
Bst4CI ACNGT 3 cut(s) 638, 678, 1453
BstACI GRCGYC 1 cut(s) 1149
BstAFI CTTAAG 1 cut(s) 529
BstAUI TGTACA 1 cut(s) 783
BstBI TTCGAA 1 cut(s) 1431
BstC8I GCNNGC 3 cut(s) 52, 959, 1102
BstDEI CTNAG 6 cut(s) 389, 449, 807, 1080, 1263, 1326
BstDSI CCRYGG 1 cut(s) 626
BstF5I GGATG 2 cut(s) 343, 920
BstHHI GCGC 1 cut(s) 1141
BstMAI GTCTC 4 cut(s) 843, 936, 1203, 1469
BstNI CCWGG 1 cut(s) 672
BstNSI RCATGY 1 cut(s) 961
BstSCI CCNGG 1 cut(s) 670
BstV1I GCAGC 6 cut(s) 172, 703, 706, 757, 779, 871
BstV2I GAAGAC 1 cut(s) 516
BstX2I RGATCY 3 cut(s) 14, 490, 925
BstYI RGATCY 3 cut(s) 14, 490, 925
BsuI GTATCC 1 cut(s) 214
BtgI CCRYGG 1 cut(s) 626
BtsCI GGATG 2 cut(s) 343, 920
BtsI GCAGTG 1 cut(s) 76
BtsIMutI CAGTG 3 cut(s) 76, 604, 841
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 3 cut(s) 52, 959, 1102
CaiI CAGNNNCTG 1 cut(s) 773
CfoI GCGC 1 cut(s) 1141
Cfr10I RCCGGY 1 cut(s) 707
Cfr13I GGNCC 2 cut(s) 206, 1160
CseI GACGC 3 cut(s) 153, 1124, 1138
Csp6I GTAC 4 cut(s) 90, 421, 723, 784
CviAII CATG 7 cut(s) 360, 627, 764, 922, 958, 1179, 1410
CviQI GTAC 4 cut(s) 90, 421, 723, 784
DdeI CTNAG 6 cut(s) 389, 449, 807, 1080, 1263, 1326
DraI TTTAAA 1 cut(s) 1095
DrdI GACNNNNNNGTC 1 cut(s) 835
DseDI GACNNNNNNGTC 1 cut(s) 835
EciI GGCGGA 1 cut(s) 1337
Eco130I CCWWGG 1 cut(s) 626
Eco31I GGTCTC 2 cut(s) 1203, 1469
Eco47I GGWCC 2 cut(s) 206, 1160
Eco57I CTGAAG 1 cut(s) 144
EcoRII CCWGG 1 cut(s) 670
EcoT14I CCWWGG 1 cut(s) 626
ErhI CCWWGG 1 cut(s) 626
FaeI CATG 7 cut(s) 363, 630, 767, 925, 961, 1182, 1413
FalI AAGNNNNNCTT 2 cut(s) 1388, 1420
FaqI GGGAC 3 cut(s) 166, 572, 1136
FatI CATG 7 cut(s) 359, 626, 763, 921, 957, 1178, 1409
FauI CCCGC 1 cut(s) 704
FauNDI CATATG 1 cut(s) 215
FbaI TGATCA 1 cut(s) 283
Fnu4HI GCNGC 6 cut(s) 186, 692, 695, 768, 771, 885
FokI GGATG 2 cut(s) 350, 927
Fsp4HI GCNGC 6 cut(s) 186, 692, 695, 768, 771, 885
FspBI CTAG 4 cut(s) 26, 126, 833, 1421
GlaI GCGC 1 cut(s) 1140
GluI GCNGC 6 cut(s) 186, 692, 695, 768, 771, 885
GsaI CCCAGC 1 cut(s) 137
HapII CCGG 1 cut(s) 708
HgaI GACGC 3 cut(s) 153, 1124, 1138
HhaI GCGC 1 cut(s) 1141
Hin1I GRCGYC 1 cut(s) 1149
Hin1II CATG 7 cut(s) 363, 630, 767, 925, 961, 1182, 1413
Hin6I GCGC 1 cut(s) 1139
HinP1I GCGC 1 cut(s) 1139
HincII GTYRAC 1 cut(s) 1336
HindII GTYRAC 1 cut(s) 1336
HinfI GANTC 2 cut(s) 414, 716
HpaII CCGG 1 cut(s) 708
HphI GGTGA 2 cut(s) 284, 602
Hpy166II GTNNAC 4 cut(s) 403, 1163, 1336, 1390
Hpy188I TCNGA 5 cut(s) 244, 810, 1022, 1083, 1329
Hpy188III TCNNGA 3 cut(s) 366, 557, 1540
Hpy8I GTNNAC 4 cut(s) 403, 1163, 1336, 1390
HpyAV CCTTC 6 cut(s) 173, 188, 404, 1373, 1376, 1390
HpyCH4III ACNGT 3 cut(s) 638, 678, 1453
HpyCH4IV ACGT 2 cut(s) 540, 614
HpyCH4V TGCA 8 cut(s) 10, 50, 653, 705, 767, 1067, 1100, 1409
HpyF3I CTNAG 6 cut(s) 389, 449, 807, 1080, 1263, 1326
HpySE526I ACGT 2 cut(s) 540, 614
Hsp92I GRCGYC 1 cut(s) 1149
Hsp92II CATG 7 cut(s) 363, 630, 767, 925, 961, 1182, 1413
HspAI GCGC 1 cut(s) 1139
Ksp22I TGATCA 1 cut(s) 283
LmnI GCTCC 1 cut(s) 568
Lsp1109I GCAGC 6 cut(s) 172, 703, 706, 757, 779, 871
LweI GCATC 2 cut(s) 328, 812
MaeI CTAG 4 cut(s) 26, 126, 833, 1421
MaeII ACGT 2 cut(s) 540, 614
MaeIII GTNAC 6 cut(s) 372, 472, 575, 826, 1011, 1445
MboII GAAGA 4 cut(s) 317, 334, 485, 521
MflI RGATCY 3 cut(s) 14, 490, 925
MluCI AATT 8 cut(s) 331, 584, 700, 855, 989, 1045, 1203, 1295
MlyI GAGTC 1 cut(s) 423
MmeI TCCRAC 1 cut(s) 952
MnlI CCTC 6 cut(s) 125, 197, 1089, 1300, 1364, 1547
MseI TTAA 2 cut(s) 530, 1094
MspA1I CMGCKG 1 cut(s) 770
MspCI CTTAAG 1 cut(s) 529
MspI CCGG 1 cut(s) 708
MspR9I CCNGG 1 cut(s) 672
MvaI CCWGG 1 cut(s) 672
NcoI CCATGG 1 cut(s) 626
NdeI CATATG 1 cut(s) 215
NlaIII CATG 7 cut(s) 363, 630, 767, 925, 961, 1182, 1413
NlaIV GGNNCC 2 cut(s) 669, 927
NmeAIII GCCGAG 1 cut(s) 1493
NmuCI GTSAC 2 cut(s) 472, 826
NspI RCATGY 1 cut(s) 961
NspV TTCGAA 1 cut(s) 1431
PaeI GCATGC 1 cut(s) 961
PfeI GAWTC 1 cut(s) 716
PkrI GCNGC 6 cut(s) 187, 693, 696, 769, 772, 886
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
PsiI TTATAA 1 cut(s) 300
Psp1406I AACGTT 1 cut(s) 540
Psp6I CCWGG 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 2 cut(s) 669, 927
PspPI GGNCC 2 cut(s) 206, 1160
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 3 cut(s) 14, 490, 925
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 4 cut(s) 91, 422, 724, 785
RsaNI GTAC 4 cut(s) 90, 421, 723, 784
SaqAI TTAA 2 cut(s) 530, 1094
SatI GCNGC 6 cut(s) 186, 692, 695, 768, 771, 885
Sau96I GGNCC 2 cut(s) 206, 1160
ScaI AGTACT 2 cut(s) 91, 422
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 1 cut(s) 672
SfaNI GCATC 2 cut(s) 328, 812
SfuI TTCGAA 1 cut(s) 1431
SgrAI CRCCGGYG 1 cut(s) 707
SinI GGWCC 2 cut(s) 206, 1160
SmlI CTYRAG 3 cut(s) 529, 1304, 1538
SmoI CTYRAG 3 cut(s) 529, 1304, 1538
SphI GCATGC 1 cut(s) 961
Sse9I AATT 8 cut(s) 331, 584, 700, 855, 989, 1045, 1203, 1295
SsiI CCGC 4 cut(s) 522, 566, 711, 1348
SspMI CTAG 4 cut(s) 26, 126, 833, 1421
StyD4I CCNGG 1 cut(s) 670
StyI CCWWGG 1 cut(s) 626
TaaI ACNGT 3 cut(s) 638, 678, 1453
TaiI ACGT 2 cut(s) 543, 617
TaqI TCGA 2 cut(s) 1431, 1456
TaqII GACCGA 1 cut(s) 223
TasI AATT 8 cut(s) 331, 584, 700, 855, 989, 1045, 1203, 1295
TatI WGTACW 3 cut(s) 89, 420, 783
TfiI GAWTC 1 cut(s) 716
Tru1I TTAA 2 cut(s) 530, 1094
Tru9I TTAA 2 cut(s) 530, 1094
TscAI CASTG 3 cut(s) 76, 611, 848
TseFI GTSAC 2 cut(s) 472, 826
TseI GCWGC 6 cut(s) 185, 691, 694, 767, 770, 884
Tsp45I GTSAC 2 cut(s) 472, 826
TspDTI ATGAA 9 cut(s) 133, 192, 335, 344, 456, 473, 838, 910, 1164
TspGWI ACGGA 1 cut(s) 954
TspRI CASTG 3 cut(s) 76, 611, 848
Vha464I CTTAAG 1 cut(s) 529
VpaK11BI GGWCC 2 cut(s) 206, 1160
XapI RAATTY 3 cut(s) 331, 584, 989
XceI RCATGY 1 cut(s) 961
XspI CTAG 4 cut(s) 26, 126, 833, 1421
ZrmI AGTACT 2 cut(s) 91, 422
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.