MD03G1204600.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000117
Physical Location & Seq
Reverse (-)
27959722 .. 27965096
5375 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1204600.v1.1.491

Sequence Viewer

Length: 1971 bp
ATGGCAATGAAATATTCAAGATCTTTGCTCTTTGGTGTGCTGCTACTCATTGGCTTTGCATTGACAAATAGCAAAGCTGCTAATACGGGTCCACCTGTTTATTGCGAATTCCTCAATCGCAACAGTTTTGAACCAGGGTTCATATTTGGCACAGGTTCTGCATCTTATCAGTATGAAGGTGCAGTAAAAGAAGATGGAAGAGGACCAAGCATATGGGATACCTACACCCACAAACATCCAGAAAAAATCGCTGATGGCAGTAACGGAGACGTTGCTATTGATCAATATCACCGCTATAAGGAAGATGTGGGGATTATGAAGGATATGGAGTTGGATGCTTACCGATTCTCTATTTCATGGTCCAGATTATTACCAAATGGAACGTTAAGTGGTGGCGTTAACAGAAAAGGAATTGATTATTACGACAATCTCATCGATGAACTCCTACGCAATGGATTAAAGCCATTTGTGACACTCTTTCATGGGGATGTTCCCCAAGCTTTAGAAGATGAATATGGTGGTTTCTTAAGCCCTCGTATTGTCGATCATTTTAAAGACTATGCGGAACTTTGTTATAAGGAATTTGGTGATCGAGTCAAGCACTGGTTCACGGAAAATGAGCCATATACTTTTAGTTACATGGGTTATGCTGTTGGTACTCAAGTACCGGGACGCTGCTCTTCTTGGCAAAACCTAAACTGCACCGGTGGAGATTCAGCCATTGAACCATACTTGGTGGCACACCACCTTCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAAGAATAGATATCAGGCAACTCAAAAAGGCTTGATAGGGATAACATTGGTGTCAGACTGGTTTGAGCCTGCTTCGGATTCAAAGCAAGATAAAGATGCTGCCTTACGATCTTTGGATTTTATGTTTGGATGGCAATATGAAGGTGCAGTAAAAGAAGATGGAAGAGGACCAAGCATATGGGATACCTACACCCCACAAACATCCAGGTCTAAAAAAATCGCTGATGGCAGTAACGGAGACGTTGCTATTGATCAATATCACCGCTATAAGGAAGATGTGGGGATTATGAAGGATATGGAGTTGGATGCTTACCGATTCTCTATTTCATGGTCCAGATTATTACCAAATGGAACGTTAAGTGGTGGCGTTAACAGAAAAGGAATTGATTATTACGACAATCTCATCGATGAACTCCTACGCAATGGATTAAAGCCATTTGTGACACTCTTTCATGGGGATGTTCCCCAAGCTTTAGAAGATGAATATGGTGGTTTCTTAGCCCAGCCTAGCTCCTCGTATTGTACTATGCGGAACTTTGTTATAAGGAATTTGGTGATCGAGTCAAGCACTGGTTCACGGGAAAATGAGCCATATACTTTTAGTTACATGGGTTATGCTGTTGGTACTCAAGTACCGGGACGCTGCTCTCTCTTGGCAAAACCTAAACTGCAACCGGTGGAGATTCAGCCATTGAACCATACTTGGTGGCACACCACCTTCCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAAGAATAGATATCAGGCAACTCAAAAAGGCTTGATAGGGATAACATTGGTGTCAGACTGGTTTGAGCCTGCTTCGGATTCAAAGCAAGATAAAGATGCTGGCCTTACGATCTTTGGATTTTATGTTTGGATGGCAACTGAGCTTAATGGAGCACTCATTGGTCCACAGGCTGCTTCAGATTGGTTATATGTATATCCAAAAGGAATTCACGATCTTGTGCTTTACACAAAGGAAAAATATAATGATCCACTCATTTATATTACTGAGAATGGTGTATCAGAGTTGAATAATCCAGAACTATCACTTGAAGAGGCCCTTCATGATACCAGTAGAATTGACTACTACTATCGTCACCTGTGTTACCTTCAAGCGGCGATCAAAAATGGCGCGAAAGTGAAGGGATACTTTGCATGGACATTGTTAGAACGACTTTGA

Protein Analysis

657

Amino Acids

74.09

Weight (kDa)

5.41

Isoelectric Point (pI)

27.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 40 - 306 1.4e-92 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 305 - 545 1.5e-59 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 566 - 654 1.4e-19 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 576, 1325
AccII CGCG 1 cut(s) 1925
AciI CCGC 5 cut(s) 292, 563, 1045, 1312, 1907
AclI AACGTT 2 cut(s) 383, 1136
AclWI GGATC 1 cut(s) 1775
AcsI RAATTY 4 cut(s) 107, 581, 1330, 1740
AcuI CTGAAG 1 cut(s) 1695
AfaI GTAC 7 cut(s) 658, 666, 782, 1306, 1408, 1416, 1535
AfiI CCNNNNNNNGG 3 cut(s) 298, 1051, 1906
AflII CTTAAG 1 cut(s) 526
AgeI ACCGGT 2 cut(s) 704, 1456
AgsI TTSAA 9 cut(s) 18, 131, 725, 864, 1477, 1617, 1822, 1844, 1904
AjnI CCWGG 2 cut(s) 133, 986
AluBI AGCT 7 cut(s) 77, 500, 770, 1253, 1293, 1523, 1678
AluI AGCT 7 cut(s) 77, 500, 770, 1253, 1293, 1523, 1678
Alw21I GWGCWC 1 cut(s) 1690
Alw26I GTCTC 2 cut(s) 261, 1014
AlwI GGATC 1 cut(s) 1775
AlwNI CAGNNNCTG 1 cut(s) 158
AoxI GGCC 2 cut(s) 1636, 1848
ApeKI GCWGC 8 cut(s) 40, 77, 675, 767, 881, 1425, 1520, 1706
ApoI RAATTY 4 cut(s) 107, 581, 1330, 1740
AsiGI ACCGGT 2 cut(s) 704, 1456
AspLEI GCGC 1 cut(s) 1925
AspS9I GGNCC 7 cut(s) 89, 203, 360, 950, 1113, 1697, 1849
AsuC2I CCSGG 2 cut(s) 669, 1419
AsuHPI GGTGA 5 cut(s) 281, 599, 1034, 1348, 1880
AvaII GGWCC 6 cut(s) 89, 203, 360, 950, 1113, 1697
BaeI ACNNNNGTAYC 8 cut(s) 648, 648, 681, 681, 1398, 1398, 1431, 1431
Bbv12I GWGCWC 1 cut(s) 1690
BbvI GCAGC 8 cut(s) 27, 64, 662, 779, 868, 1412, 1532, 1693
BccI CCATC 6 cut(s) 188, 248, 906, 935, 1001, 1660
BciT130I CCWGG 2 cut(s) 135, 988
BciVI GTATCC 3 cut(s) 211, 958, 1931
BclI TGATCA 2 cut(s) 280, 1033
BcnI CCSGG 2 cut(s) 669, 1419
BcoDI GTCTC 2 cut(s) 261, 1014
BfaI CTAG 1 cut(s) 1290
BfmI CTRYAG 2 cut(s) 771, 1524
BfrI CTTAAG 1 cut(s) 526
BfuI GTATCC 3 cut(s) 211, 958, 1931
BglII AGATCT 1 cut(s) 20
BisI GCNGC 9 cut(s) 41, 78, 676, 768, 882, 1426, 1521, 1707, 1908
BlsI GCNGC 9 cut(s) 42, 79, 677, 769, 883, 1427, 1522, 1708, 1909
Bme1390I CCNGG 4 cut(s) 135, 669, 988, 1419
Bme18I GGWCC 6 cut(s) 89, 203, 360, 950, 1113, 1697
BmgT120I GGNCC 7 cut(s) 89, 203, 360, 950, 1113, 1697, 1849
BmiI GGNNCC 1 cut(s) 90
BmrFI CCNGG 4 cut(s) 135, 669, 988, 1419
BmsI GCATC 5 cut(s) 170, 325, 868, 1078, 1621
BpuEI CTTGAG 2 cut(s) 645, 1395
BpuMI CCSGG 2 cut(s) 669, 1419
Bsa29I ATCGAT 2 cut(s) 435, 1188
BsaBI GATNNNNATC 2 cut(s) 285, 1038
BsaJI CCNNGG 1 cut(s) 134
BsaWI WCCGGW 2 cut(s) 704, 1456
BsaXI ACNNNNNCTCC 4 cut(s) 756, 786, 1509, 1539
Bsc4I CCNNNNNNNGG 3 cut(s) 298, 1051, 1906
Bse118I RCCGGY 2 cut(s) 704, 1456
Bse1I ACTGG 5 cut(s) 608, 845, 1357, 1598, 1863
Bse3DI GCAATG 3 cut(s) 12, 457, 1210
Bse8I GATNNNNATC 2 cut(s) 285, 1038
BseBI CCWGG 2 cut(s) 135, 988
BseCI ATCGAT 2 cut(s) 435, 1188
BseDI CCNNGG 1 cut(s) 134
BseGI GGATG 8 cut(s) 235, 340, 493, 917, 983, 1093, 1246, 1671
BseJI GATNNNNATC 2 cut(s) 285, 1038
BseLI CCNNNNNNNGG 3 cut(s) 298, 1051, 1906
BseMI GCAATG 3 cut(s) 12, 457, 1210
BseMII CTCAG 2 cut(s) 1665, 1791
BseNI ACTGG 5 cut(s) 608, 845, 1357, 1598, 1863
BseRI GAGGAG 1 cut(s) 1285
BseXI GCAGC 8 cut(s) 27, 64, 662, 779, 868, 1412, 1532, 1693
BseYI CCCAGC 1 cut(s) 1284
BsgI GTGCAG 3 cut(s) 201, 685, 948
Bsh1236I CGCG 1 cut(s) 1925
BshFI GGCC 2 cut(s) 1638, 1850
BshTI ACCGGT 2 cut(s) 704, 1456
BshVI ATCGAT 2 cut(s) 435, 1188
BsiHKAI GWGCWC 1 cut(s) 1690
BsiSI CCGG 4 cut(s) 668, 705, 1418, 1457
BslFI GGGAC 2 cut(s) 684, 1434
BslI CCNNNNNNNGG 3 cut(s) 298, 1051, 1906
BsmAI GTCTC 2 cut(s) 261, 1014
BsmBI CGTCTC 2 cut(s) 261, 1014
BsmFI GGGAC 2 cut(s) 684, 1434
BsnI GGCC 2 cut(s) 1638, 1850
Bsp1286I GDGCHC 1 cut(s) 1690
Bsp1407I TGTACA 2 cut(s) 780, 1533
BspACI CCGC 5 cut(s) 292, 563, 1045, 1312, 1907
BspANI GGCC 2 cut(s) 1638, 1850
BspCNI CTCAG 2 cut(s) 1666, 1792
BspDI ATCGAT 2 cut(s) 435, 1188
BspFNI CGCG 1 cut(s) 1925
BspHI TCATGA 1 cut(s) 1855
BspLI GGNNCC 1 cut(s) 90
BspPI GGATC 1 cut(s) 1775
BspQI GCTCTTC 1 cut(s) 685
BspTI CTTAAG 1 cut(s) 526
BsrDI GCAATG 3 cut(s) 12, 457, 1210
BsrFI RCCGGY 2 cut(s) 704, 1456
BsrGI TGTACA 2 cut(s) 780, 1533
BsrI ACTGG 5 cut(s) 608, 845, 1357, 1598, 1863
BssAI RCCGGY 2 cut(s) 704, 1456
BssECI CCNNGG 1 cut(s) 134
Bst2UI CCWGG 2 cut(s) 135, 988
Bst4CI ACNGT 1 cut(s) 125
Bst6I CTCTTC 4 cut(s) 193, 685, 940, 1839
BstAFI CTTAAG 1 cut(s) 526
BstAUI TGTACA 2 cut(s) 780, 1533
BstC8I GCNNGC 3 cut(s) 852, 1605, 1636
BstDEI CTNAG 3 cut(s) 1279, 1674, 1800
BstF5I GGATG 8 cut(s) 235, 340, 493, 917, 983, 1093, 1246, 1671
BstFNI CGCG 1 cut(s) 1925
BstHHI GCGC 1 cut(s) 1925
BstMAI GTCTC 2 cut(s) 261, 1014
BstMWI GCNNNNNNNGC 2 cut(s) 764, 1517
BstNI CCWGG 2 cut(s) 135, 988
BstSCI CCNGG 4 cut(s) 133, 667, 986, 1417
BstSFI CTRYAG 2 cut(s) 771, 1524
BstUI CGCG 1 cut(s) 1925
BstV1I GCAGC 8 cut(s) 27, 64, 662, 779, 868, 1412, 1532, 1693
BstX2I RGATCY 1 cut(s) 20
BstXI CCANNNNNNTGG 2 cut(s) 213, 960
BstYI RGATCY 1 cut(s) 20
Bsu15I ATCGAT 2 cut(s) 435, 1188
BsuI GTATCC 3 cut(s) 211, 958, 1931
BsuRI GGCC 2 cut(s) 1638, 1850
BsuTUI ATCGAT 2 cut(s) 435, 1188
BtsCI GGATG 8 cut(s) 235, 340, 493, 917, 983, 1093, 1246, 1671
BtsIMutI CAGTG 2 cut(s) 601, 1350
Cac8I GCNNGC 3 cut(s) 852, 1605, 1636
CaiI CAGNNNCTG 1 cut(s) 158
CciI TCATGA 1 cut(s) 1855
CfoI GCGC 1 cut(s) 1925
Cfr10I RCCGGY 2 cut(s) 704, 1456
Cfr13I GGNCC 7 cut(s) 89, 203, 360, 950, 1113, 1697, 1849
ClaI ATCGAT 2 cut(s) 435, 1188
CseI GACGC 2 cut(s) 681, 1431
Csp6I GTAC 7 cut(s) 657, 665, 781, 1305, 1407, 1415, 1534
CspAI ACCGGT 2 cut(s) 704, 1456
CviQI GTAC 7 cut(s) 657, 665, 781, 1305, 1407, 1415, 1534
DdeI CTNAG 3 cut(s) 1279, 1674, 1800
DraI TTTAAA 1 cut(s) 553
Eam1104I CTCTTC 4 cut(s) 193, 685, 940, 1839
EarI CTCTTC 4 cut(s) 193, 685, 940, 1839
Eco32I GATATC 2 cut(s) 794, 1547
Eco47I GGWCC 6 cut(s) 89, 203, 360, 950, 1113, 1697
Eco57I CTGAAG 1 cut(s) 1695
EcoO109I RGGNCCY 1 cut(s) 1849
EcoRI GAATTC 2 cut(s) 107, 1740
EcoRII CCWGG 2 cut(s) 133, 986
EcoRV GATATC 2 cut(s) 794, 1547
Esp3I CGTCTC 2 cut(s) 261, 1014
FalI AAGNNNNNCTT 4 cut(s) 1836, 1868, 1925, 1957
FaqI GGGAC 2 cut(s) 684, 1434
FauNDI CATATG 2 cut(s) 212, 959
FbaI TGATCA 2 cut(s) 280, 1033
Fnu4HI GCNGC 9 cut(s) 41, 78, 676, 768, 882, 1426, 1521, 1707, 1908
FokI GGATG 8 cut(s) 222, 347, 500, 924, 970, 1100, 1253, 1678
Fsp4HI GCNGC 9 cut(s) 41, 78, 676, 768, 882, 1426, 1521, 1707, 1908
FspBI CTAG 1 cut(s) 1290
GlaI GCGC 1 cut(s) 1924
GluI GCNGC 9 cut(s) 41, 78, 676, 768, 882, 1426, 1521, 1707, 1908
GsaI CCCAGC 1 cut(s) 1288
HaeIII GGCC 2 cut(s) 1638, 1850
HapII CCGG 4 cut(s) 668, 705, 1418, 1457
HgaI GACGC 2 cut(s) 681, 1431
HhaI GCGC 1 cut(s) 1925
Hin6I GCGC 1 cut(s) 1923
HinP1I GCGC 1 cut(s) 1923
HincII GTYRAC 2 cut(s) 400, 1153
HindII GTYRAC 2 cut(s) 400, 1153
HindIII AAGCTT 2 cut(s) 498, 1251
HinfI GANTC 8 cut(s) 345, 594, 713, 860, 1098, 1343, 1465, 1613
HpaI GTTAAC 2 cut(s) 400, 1153
HpaII CCGG 4 cut(s) 668, 705, 1418, 1457
HphI GGTGA 5 cut(s) 281, 599, 1034, 1348, 1880
Hpy166II GTNNAC 6 cut(s) 92, 400, 609, 1153, 1358, 1700
Hpy188I TCNGA 6 cut(s) 838, 859, 1591, 1612, 1714, 1816
Hpy188III TCNNGA 7 cut(s) 18, 239, 363, 1116, 1745, 1829, 1856
Hpy8I GTNNAC 6 cut(s) 92, 400, 609, 1153, 1358, 1700
HpyCH4III ACNGT 1 cut(s) 125
HpyCH4IV ACGT 4 cut(s) 270, 383, 1023, 1136
HpyCH4V TGCA 7 cut(s) 59, 161, 182, 702, 929, 1453, 1946
HpyF10VI GCNNNNNNNGC 2 cut(s) 764, 1517
HpyF3I CTNAG 3 cut(s) 1279, 1674, 1800
HpySE526I ACGT 4 cut(s) 270, 383, 1023, 1136
HspAI GCGC 1 cut(s) 1923
Ksp22I TGATCA 2 cut(s) 280, 1033
KspAI GTTAAC 2 cut(s) 400, 1153
LguI GCTCTTC 1 cut(s) 685
LmnI GCTCC 4 cut(s) 764, 1298, 1517, 1685
Lsp1109I GCAGC 8 cut(s) 27, 64, 662, 779, 868, 1412, 1532, 1693
LweI GCATC 5 cut(s) 170, 325, 868, 1078, 1621
MaeI CTAG 1 cut(s) 1290
MaeII ACGT 4 cut(s) 270, 383, 1023, 1136
MaeIII GTNAC 8 cut(s) 260, 469, 635, 1013, 1222, 1385, 1886, 1895
MflI RGATCY 1 cut(s) 20
MhlI GDGCHC 1 cut(s) 1690
MluCI AATT 9 cut(s) 107, 411, 581, 776, 1164, 1330, 1529, 1740, 1869
MlyI GAGTC 2 cut(s) 603, 1352
MmeI TCCRAC 2 cut(s) 312, 1065
MnlI CCTC 6 cut(s) 122, 194, 543, 941, 1306, 1840
MseI TTAA 9 cut(s) 386, 399, 458, 527, 552, 1139, 1152, 1211, 1680
MslI CAYNNNNRTG 2 cut(s) 486, 1239
MspA1I CMGCKG 2 cut(s) 770, 1523
MspCI CTTAAG 1 cut(s) 526
MspI CCGG 4 cut(s) 668, 705, 1418, 1457
MspR9I CCNGG 4 cut(s) 135, 669, 988, 1419
MvaI CCWGG 2 cut(s) 135, 988
MvnI CGCG 1 cut(s) 1925
MwoI GCNNNNNNNGC 2 cut(s) 764, 1517
NciI CCSGG 2 cut(s) 669, 1419
NdeI CATATG 2 cut(s) 212, 959
NlaIV GGNNCC 1 cut(s) 90
NmuCI GTSAC 3 cut(s) 469, 1222, 1886
PagI TCATGA 1 cut(s) 1855
PciSI GCTCTTC 1 cut(s) 685
PfeI GAWTC 6 cut(s) 345, 713, 860, 1098, 1465, 1613
PinAI ACCGGT 2 cut(s) 704, 1456
PkrI GCNGC 9 cut(s) 42, 79, 677, 769, 883, 1427, 1522, 1708, 1909
PleI GAGTC 2 cut(s) 602, 1351
PpsI GAGTC 2 cut(s) 602, 1351
PsiI TTATAA 2 cut(s) 576, 1325
Psp1406I AACGTT 2 cut(s) 383, 1136
Psp6I CCWGG 2 cut(s) 133, 986
PspFI CCCAGC 1 cut(s) 1284
PspGI CCWGG 2 cut(s) 133, 986
PspN4I GGNNCC 1 cut(s) 90
PspPI GGNCC 7 cut(s) 89, 203, 360, 950, 1113, 1697, 1849
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 1 cut(s) 20
PvuII CAGCTG 2 cut(s) 770, 1523
RsaI GTAC 7 cut(s) 658, 666, 782, 1306, 1408, 1416, 1535
RsaNI GTAC 7 cut(s) 657, 665, 781, 1305, 1407, 1415, 1534
RseI CAYNNNNRTG 2 cut(s) 486, 1239
SapI GCTCTTC 1 cut(s) 685
SaqAI TTAA 9 cut(s) 386, 399, 458, 527, 552, 1139, 1152, 1211, 1680
SatI GCNGC 9 cut(s) 41, 78, 676, 768, 882, 1426, 1521, 1707, 1908
Sau96I GGNCC 7 cut(s) 89, 203, 360, 950, 1113, 1697, 1849
SchI GAGTC 2 cut(s) 603, 1352
ScrFI CCNGG 4 cut(s) 135, 669, 988, 1419
SduI GDGCHC 1 cut(s) 1690
SfaNI GCATC 5 cut(s) 170, 325, 868, 1078, 1621
SfcI CTRYAG 2 cut(s) 771, 1524
SgrAI CRCCGGYG 1 cut(s) 704
SinI GGWCC 6 cut(s) 89, 203, 360, 950, 1113, 1697
SmiMI CAYNNNNRTG 2 cut(s) 486, 1239
SmlI CTYRAG 3 cut(s) 526, 660, 1410
SmoI CTYRAG 3 cut(s) 526, 660, 1410
Sse9I AATT 9 cut(s) 107, 411, 581, 776, 1164, 1330, 1529, 1740, 1869
SsiI CCGC 5 cut(s) 292, 563, 1045, 1312, 1907
SspI AATATT 1 cut(s) 14
SspMI CTAG 1 cut(s) 1290
StyD4I CCNGG 4 cut(s) 133, 667, 986, 1417
TaaI ACNGT 1 cut(s) 125
TaiI ACGT 4 cut(s) 273, 386, 1026, 1139
TaqI TCGA 5 cut(s) 435, 543, 592, 1188, 1341
TasI AATT 9 cut(s) 107, 411, 581, 776, 1164, 1330, 1529, 1740, 1869
TatI WGTACW 3 cut(s) 780, 1304, 1533
TauI GCSGC 1 cut(s) 1910
TfiI GAWTC 6 cut(s) 345, 713, 860, 1098, 1465, 1613
Tru1I TTAA 9 cut(s) 386, 399, 458, 527, 552, 1139, 1152, 1211, 1680
Tru9I TTAA 9 cut(s) 386, 399, 458, 527, 552, 1139, 1152, 1211, 1680
TscAI CASTG 2 cut(s) 608, 1357
TseFI GTSAC 3 cut(s) 469, 1222, 1886
TseI GCWGC 8 cut(s) 40, 77, 675, 767, 881, 1425, 1520, 1706
Tsp45I GTSAC 3 cut(s) 469, 1222, 1886
TspGWI ACGGA 3 cut(s) 279, 626, 1032
TspRI CASTG 2 cut(s) 608, 1357
Vha464I CTTAAG 1 cut(s) 526
VpaK11BI GGWCC 6 cut(s) 89, 203, 360, 950, 1113, 1697
XapI RAATTY 4 cut(s) 107, 581, 1330, 1740
XspI CTAG 1 cut(s) 1290
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.