MD00G1145200.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000117
Physical Location & Seq
Reverse (-)
31766072 .. 31769520
3449 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1145200.v1.1.491

Sequence Viewer

Length: 1635 bp
ATGCAGTATTCCTGGAAGAGACAACTTAATTATCACGATCGATATCAAAATTCCCAAGTTATGGCAATGAAATATTCAAGATCTTTGCTCTTTGGTGTGCTGCTACTCATTGGCTTTGCATTGACAAATAGCAAAGCTGCTAATACGGATCCACCTGTTCATTGTGAATTTCTCAATCGCAACAGTTTTGAACCAGGGTTCATATTTGGTACAGGTTCTGCATCTTACCAGTATGAAGGTGCAGTAAAAGAAGATGGAAGAGGACCAAGCATATGGGATACCTACACCCACAAACATCCAGAAAAAATCACCGATGGCAGTAACGGAGACGTTGCTATTGATCAATATCACCGCTATAAGGAAGATGTGGGGATTATGAAGGATATGGAGTTGGATGCTTACCGATTCTCTATTTCATGGTCCAGATTATTACCAAATGGAACGTTAAGTGGTGGCGTTAACATAAAAGGAATTGATTATTACAACAATCTCATCGATGAACTCCTACGCAATGGATTAAAGCCATTTGTGACACTCTTTCATGGGGATGTTCCCCAAGCTTTAGAAGATGAATATGGTGGTTTCTTAAGCCCTCGTATTGTCGAACATTTTAAAGACTATGCGGAACTTTGTTATAAGGAATTTGGTGATCGAGTCAAGCACTGGTTCACGGAAAATGAGCCATATACTTTTAGTTACATGGGTTATGCTGTTGGTACTCAAGTACCGGGACGTTGCTCTTCTTGGCAAAACCTAAACTGCACCGGTGGAGATTCAGCCAGTGAACCATACTTGGTGGCACACCACCTTCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAAGAATAGATATCAGGCAACTCAAAAAGGCTTGATAGGGATAACATTGGTGTCAGACTGGTTTGAGCCTGCTTCAGATTCAAAGCAAGATAAAGATGCTGCCTTACGATCTTTGGATTTTATGTTTGGATGGTTTTTGGACCCATTAACAAGTGGTGACTATCCACACACCATGCGATCAATTGTTGGGAAAAGATTGCCCAAATTCACAAAAGAACAATCCAAGTTGCTAAACGGATCATTTGATTTTCTTGGAATAAATTATTATACTGCTAGATACACAAGTAGTGCACCCAAGAACAATTCACTACCGGCAAGCTACGTAACAGATTCTCGTGCTGATCTTACAACTGAGCTTAATGGAGTACTCATTGGTCCACAGGCTGCTTCGGATTGGTTATATGTATATCCAAAAGGAATTCACGATCTTGTGCTTTACACAAAGGAAAAATATAATGATCCACTCATTTATATTACTGAGAATGGTGTATCAGAGTTGAATAATCCAGAACTATCACTTGAAGAGGCCCTTCATGATACCAGTAGAATTGACTACTACTATCGTCACCTGTGTTACCTTCAAGCGGCGATCAAAAATGGCGCGAAAGTGAAGGGATACTTTGCATGGACATTGTTAGACAACTTTGAATGGACTTCTGGATACACTGTCCGATTTGGTTTAAACTACGTGGATTATAAAGATGGGCTGAAAAGATACCCAAAACTCTCAGCACACTGGTTCAAAAATTTGCTTAAGAAGAATAAATTCGAAGAAAGCTATAGTTCATTATAA

Protein Analysis

545

Amino Acids

62.19

Weight (kDa)

6.16

Isoelectric Point (pI)

25.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 60 - 536 2.1e-156 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 636, 1539, 1633
AccB7I CCANNNNNTGG 1 cut(s) 61
AccII CGCG 1 cut(s) 1445
AciI CCGC 3 cut(s) 352, 623, 1427
AclI AACGTT 1 cut(s) 443
AclWI GGATC 4 cut(s) 143, 156, 1087, 1295
AcsI RAATTY 7 cut(s) 49, 167, 641, 1046, 1260, 1588, 1607
AcuI CTGAAG 1 cut(s) 900
AfaI GTAC 5 cut(s) 211, 718, 726, 842, 1209
AfiI CCNNNNNNNGG 3 cut(s) 61, 358, 1426
AflII CTTAAG 2 cut(s) 586, 1595
AgeI ACCGGT 1 cut(s) 764
AgsI TTSAA 8 cut(s) 78, 191, 924, 1342, 1364, 1424, 1490, 1585
AjnI CCWGG 2 cut(s) 11, 193
AluBI AGCT 6 cut(s) 137, 560, 830, 1161, 1198, 1620
AluI AGCT 6 cut(s) 137, 560, 830, 1161, 1198, 1620
Alw21I GWGCWC 1 cut(s) 1135
Alw26I GTCTC 2 cut(s) 13, 321
Alw44I GTGCAC 1 cut(s) 1131
AlwI GGATC 4 cut(s) 143, 156, 1087, 1295
AlwNI CAGNNNCTG 1 cut(s) 218
AoxI GGCC 1 cut(s) 1368
ApaLI GTGCAC 1 cut(s) 1131
ApeKI GCWGC 5 cut(s) 100, 137, 827, 941, 1226
ApoI RAATTY 7 cut(s) 49, 167, 641, 1046, 1260, 1588, 1607
AsiGI ACCGGT 1 cut(s) 764
Asp700I GAANNNNTTC 1 cut(s) 1607
AspLEI GCGC 1 cut(s) 1445
AspS9I GGNCC 5 cut(s) 263, 420, 982, 1217, 1369
AsuC2I CCSGG 1 cut(s) 729
AsuHPI GGTGA 5 cut(s) 301, 341, 659, 1010, 1400
AsuII TTCGAA 1 cut(s) 1611
AvaII GGWCC 4 cut(s) 263, 420, 982, 1217
BaeGI GKGCMC 1 cut(s) 1135
BaeI ACNNNNGTAYC 6 cut(s) 708, 708, 741, 741, 1111, 1144
BamHI GGATCC 1 cut(s) 148
BauI CACGAG 1 cut(s) 1176
Bbv12I GWGCWC 1 cut(s) 1135
BbvI GCAGC 5 cut(s) 87, 124, 839, 928, 1213
BccI CCATC 4 cut(s) 248, 308, 966, 1538
BciT130I CCWGG 2 cut(s) 13, 195
BciVI GTATCC 3 cut(s) 271, 1451, 1496
BclI TGATCA 1 cut(s) 340
BcnI CCSGG 1 cut(s) 729
BcoDI GTCTC 2 cut(s) 13, 321
BfaI CTAG 1 cut(s) 1116
BfmI CTRYAG 2 cut(s) 831, 1621
BfrI CTTAAG 2 cut(s) 586, 1595
BfuI GTATCC 3 cut(s) 271, 1451, 1496
BglII AGATCT 1 cut(s) 80
BisI GCNGC 6 cut(s) 101, 138, 828, 942, 1227, 1428
BlsI GCNGC 6 cut(s) 102, 139, 829, 943, 1228, 1429
BmcAI AGTACT 1 cut(s) 1209
Bme1390I CCNGG 3 cut(s) 13, 195, 729
Bme18I GGWCC 4 cut(s) 263, 420, 982, 1217
BmgT120I GGNCC 5 cut(s) 263, 420, 982, 1217, 1369
BmiI GGNNCC 2 cut(s) 150, 984
BmrFI CCNGG 3 cut(s) 13, 195, 729
BmsI GCATC 3 cut(s) 230, 385, 928
Bpu14I TTCGAA 1 cut(s) 1611
BpuEI CTTGAG 1 cut(s) 705
BpuMI CCSGG 1 cut(s) 729
Bsa29I ATCGAT 2 cut(s) 40, 495
BsaAI YACGTR 2 cut(s) 1165, 1531
BsaBI GATNNNNATC 2 cut(s) 42, 345
BsaJI CCNNGG 1 cut(s) 194
BsaWI WCCGGW 1 cut(s) 764
BsaXI ACNNNNNCTCC 2 cut(s) 816, 846
Bsc4I CCNNNNNNNGG 3 cut(s) 61, 358, 1426
Bse118I RCCGGY 2 cut(s) 764, 1153
Bse1I ACTGG 6 cut(s) 229, 668, 780, 905, 1383, 1583
Bse3DI GCAATG 2 cut(s) 72, 517
Bse8I GATNNNNATC 2 cut(s) 42, 345
BseBI CCWGG 2 cut(s) 13, 195
BseCI ATCGAT 2 cut(s) 40, 495
BseDI CCNNGG 1 cut(s) 194
BseGI GGATG 4 cut(s) 295, 400, 553, 977
BseJI GATNNNNATC 2 cut(s) 42, 345
BseLI CCNNNNNNNGG 3 cut(s) 61, 358, 1426
BseMI GCAATG 2 cut(s) 72, 517
BseMII CTCAG 3 cut(s) 1185, 1311, 1584
BseNI ACTGG 6 cut(s) 229, 668, 780, 905, 1383, 1583
BseSI GKGCMC 1 cut(s) 1135
BseXI GCAGC 5 cut(s) 87, 124, 839, 928, 1213
BsgI GTGCAG 2 cut(s) 261, 745
Bsh1236I CGCG 1 cut(s) 1445
Bsh1285I CGRYCG 1 cut(s) 40
BshFI GGCC 1 cut(s) 1370
BshTI ACCGGT 1 cut(s) 764
BshVI ATCGAT 2 cut(s) 40, 495
BsiEI CGRYCG 1 cut(s) 40
BsiHKAI GWGCWC 1 cut(s) 1135
BsiSI CCGG 3 cut(s) 728, 765, 1154
BslFI GGGAC 1 cut(s) 744
BslI CCNNNNNNNGG 3 cut(s) 61, 358, 1426
BsmAI GTCTC 2 cut(s) 13, 321
BsmBI CGTCTC 1 cut(s) 321
BsmFI GGGAC 1 cut(s) 744
BsnI GGCC 1 cut(s) 1370
Bsp119I TTCGAA 1 cut(s) 1611
Bsp1286I GDGCHC 1 cut(s) 1135
Bsp1407I TGTACA 1 cut(s) 840
BspACI CCGC 3 cut(s) 352, 623, 1427
BspANI GGCC 1 cut(s) 1370
BspCNI CTCAG 3 cut(s) 1186, 1312, 1583
BspDI ATCGAT 2 cut(s) 40, 495
BspFNI CGCG 1 cut(s) 1445
BspHI TCATGA 1 cut(s) 1375
BspLI GGNNCC 2 cut(s) 150, 984
BspPI GGATC 4 cut(s) 143, 156, 1087, 1295
BspQI GCTCTTC 1 cut(s) 745
BspT104I TTCGAA 1 cut(s) 1611
BspTI CTTAAG 2 cut(s) 586, 1595
BsrDI GCAATG 2 cut(s) 72, 517
BsrFI RCCGGY 2 cut(s) 764, 1153
BsrGI TGTACA 1 cut(s) 840
BsrI ACTGG 6 cut(s) 229, 668, 780, 905, 1383, 1583
BssAI RCCGGY 2 cut(s) 764, 1153
BssECI CCNNGG 1 cut(s) 194
BssSI CACGAG 1 cut(s) 1176
Bst2BI CACGAG 1 cut(s) 1176
Bst2UI CCWGG 2 cut(s) 13, 195
Bst4CI ACNGT 2 cut(s) 185, 1510
Bst6I CTCTTC 4 cut(s) 11, 253, 745, 1359
BstAFI CTTAAG 2 cut(s) 586, 1595
BstAUI TGTACA 1 cut(s) 840
BstBAI YACGTR 2 cut(s) 1165, 1531
BstBI TTCGAA 1 cut(s) 1611
BstC8I GCNNGC 2 cut(s) 912, 1159
BstDEI CTNAG 3 cut(s) 1194, 1320, 1570
BstF5I GGATG 4 cut(s) 295, 400, 553, 977
BstFNI CGCG 1 cut(s) 1445
BstHHI GCGC 1 cut(s) 1445
BstMAI GTCTC 2 cut(s) 13, 321
BstMCI CGRYCG 1 cut(s) 40
BstMWI GCNNNNNNNGC 1 cut(s) 824
BstNI CCWGG 2 cut(s) 13, 195
BstSCI CCNGG 3 cut(s) 11, 193, 727
BstSFI CTRYAG 2 cut(s) 831, 1621
BstSLI GKGCMC 1 cut(s) 1135
BstSNI TACGTA 1 cut(s) 1165
BstUI CGCG 1 cut(s) 1445
BstV1I GCAGC 5 cut(s) 87, 124, 839, 928, 1213
BstX2I RGATCY 2 cut(s) 80, 148
BstXI CCANNNNNNTGG 1 cut(s) 273
BstYI RGATCY 2 cut(s) 80, 148
Bsu15I ATCGAT 2 cut(s) 40, 495
BsuI GTATCC 3 cut(s) 271, 1451, 1496
BsuRI GGCC 1 cut(s) 1370
BsuTUI ATCGAT 2 cut(s) 40, 495
BtsCI GGATG 4 cut(s) 295, 400, 553, 977
BtsIMutI CAGTG 4 cut(s) 661, 787, 1506, 1576
Cac8I GCNNGC 2 cut(s) 912, 1159
CaiI CAGNNNCTG 1 cut(s) 218
CciI TCATGA 1 cut(s) 1375
CfoI GCGC 1 cut(s) 1445
Cfr10I RCCGGY 2 cut(s) 764, 1153
Cfr13I GGNCC 5 cut(s) 263, 420, 982, 1217, 1369
ClaI ATCGAT 2 cut(s) 40, 495
Csp6I GTAC 5 cut(s) 210, 717, 725, 841, 1208
CspAI ACCGGT 1 cut(s) 764
CviAII CATG 7 cut(s) 417, 542, 700, 821, 1015, 1376, 1467
CviQI GTAC 5 cut(s) 210, 717, 725, 841, 1208
DdeI CTNAG 3 cut(s) 1194, 1320, 1570
DraI TTTAAA 2 cut(s) 613, 1524
Eam1104I CTCTTC 4 cut(s) 11, 253, 745, 1359
EarI CTCTTC 4 cut(s) 11, 253, 745, 1359
Eco105I TACGTA 1 cut(s) 1165
Eco32I GATATC 2 cut(s) 44, 854
Eco47I GGWCC 4 cut(s) 263, 420, 982, 1217
Eco57I CTGAAG 1 cut(s) 900
EcoO109I RGGNCCY 1 cut(s) 1369
EcoRI GAATTC 1 cut(s) 1260
EcoRII CCWGG 2 cut(s) 11, 193
EcoRV GATATC 2 cut(s) 44, 854
Esp3I CGTCTC 1 cut(s) 321
FaeI CATG 7 cut(s) 420, 545, 703, 824, 1018, 1379, 1470
FalI AAGNNNNNCTT 4 cut(s) 1356, 1388, 1445, 1477
FaqI GGGAC 1 cut(s) 744
FatI CATG 7 cut(s) 416, 541, 699, 820, 1014, 1375, 1466
FauNDI CATATG 1 cut(s) 272
FbaI TGATCA 1 cut(s) 340
Fnu4HI GCNGC 6 cut(s) 101, 138, 828, 942, 1227, 1428
FokI GGATG 4 cut(s) 282, 407, 560, 984
Fsp4HI GCNGC 6 cut(s) 101, 138, 828, 942, 1227, 1428
FspBI CTAG 1 cut(s) 1116
GlaI GCGC 1 cut(s) 1444
GluI GCNGC 6 cut(s) 101, 138, 828, 942, 1227, 1428
HaeIII GGCC 1 cut(s) 1370
HapII CCGG 3 cut(s) 728, 765, 1154
HhaI GCGC 1 cut(s) 1445
Hin1II CATG 7 cut(s) 420, 545, 703, 824, 1018, 1379, 1470
Hin6I GCGC 1 cut(s) 1443
HinP1I GCGC 1 cut(s) 1443
HincII GTYRAC 1 cut(s) 460
HindII GTYRAC 1 cut(s) 460
HindIII AAGCTT 1 cut(s) 558
HinfI GANTC 5 cut(s) 405, 654, 773, 920, 1172
HpaI GTTAAC 1 cut(s) 460
HpaII CCGG 3 cut(s) 728, 765, 1154
HphI GGTGA 5 cut(s) 301, 341, 659, 1010, 1400
Hpy166II GTNNAC 5 cut(s) 460, 669, 785, 1133, 1220
Hpy188I TCNGA 5 cut(s) 898, 919, 1234, 1336, 1514
Hpy188III TCNNGA 8 cut(s) 35, 78, 299, 423, 1265, 1349, 1376, 1500
Hpy8I GTNNAC 5 cut(s) 460, 669, 785, 1133, 1220
HpyAV CCTTC 6 cut(s) 230, 373, 818, 1382, 1430, 1447
HpyCH4III ACNGT 2 cut(s) 185, 1510
HpyCH4IV ACGT 5 cut(s) 330, 443, 733, 1164, 1530
HpyCH4V TGCA 7 cut(s) 4, 119, 221, 242, 762, 1133, 1466
HpyF10VI GCNNNNNNNGC 1 cut(s) 824
HpyF3I CTNAG 3 cut(s) 1194, 1320, 1570
HpySE526I ACGT 5 cut(s) 330, 443, 733, 1164, 1530
Hsp92II CATG 7 cut(s) 420, 545, 703, 824, 1018, 1379, 1470
HspAI GCGC 1 cut(s) 1443
Ksp22I TGATCA 1 cut(s) 340
KspAI GTTAAC 1 cut(s) 460
LguI GCTCTTC 1 cut(s) 745
LmnI GCTCC 1 cut(s) 824
Lsp1109I GCAGC 5 cut(s) 87, 124, 839, 928, 1213
LweI GCATC 3 cut(s) 230, 385, 928
MaeI CTAG 1 cut(s) 1116
MaeII ACGT 5 cut(s) 330, 443, 733, 1164, 1530
MaeIII GTNAC 7 cut(s) 320, 529, 695, 998, 1165, 1406, 1415
MfeI CAATTG 1 cut(s) 1023
MflI RGATCY 2 cut(s) 80, 148
MhlI GDGCHC 1 cut(s) 1135
MlyI GAGTC 1 cut(s) 663
MmeI TCCRAC 1 cut(s) 372
MnlI CCTC 3 cut(s) 254, 603, 1360
MroXI GAANNNNTTC 1 cut(s) 1607
MslI CAYNNNNRTG 1 cut(s) 546
MspA1I CMGCKG 1 cut(s) 830
MspCI CTTAAG 2 cut(s) 586, 1595
MspI CCGG 3 cut(s) 728, 765, 1154
MspR9I CCNGG 3 cut(s) 13, 195, 729
MssI GTTTAAAC 1 cut(s) 1524
MunI CAATTG 1 cut(s) 1023
MvaI CCWGG 2 cut(s) 13, 195
MvnI CGCG 1 cut(s) 1445
MwoI GCNNNNNNNGC 1 cut(s) 824
NciI CCSGG 1 cut(s) 729
NdeI CATATG 1 cut(s) 272
NlaIII CATG 7 cut(s) 420, 545, 703, 824, 1018, 1379, 1470
NlaIV GGNNCC 2 cut(s) 150, 984
NmuCI GTSAC 3 cut(s) 529, 998, 1406
NspV TTCGAA 1 cut(s) 1611
PagI TCATGA 1 cut(s) 1375
PciSI GCTCTTC 1 cut(s) 745
PdmI GAANNNNTTC 1 cut(s) 1607
PfeI GAWTC 4 cut(s) 405, 773, 920, 1172
PflMI CCANNNNNTGG 1 cut(s) 61
PfoI TCCNGGA 1 cut(s) 11
PinAI ACCGGT 1 cut(s) 764
PkrI GCNGC 6 cut(s) 102, 139, 829, 943, 1228, 1429
Ple19I CGATCG 1 cut(s) 40
PleI GAGTC 1 cut(s) 662
PmeI GTTTAAAC 1 cut(s) 1524
PpsI GAGTC 1 cut(s) 662
Ppu21I YACGTR 2 cut(s) 1165, 1531
PsiI TTATAA 3 cut(s) 636, 1539, 1633
Psp1406I AACGTT 1 cut(s) 443
Psp6I CCWGG 2 cut(s) 11, 193
PspGI CCWGG 2 cut(s) 11, 193
PspN4I GGNNCC 2 cut(s) 150, 984
PspPI GGNCC 5 cut(s) 263, 420, 982, 1217, 1369
PstNI CAGNNNCTG 1 cut(s) 218
PsuI RGATCY 2 cut(s) 80, 148
PvuI CGATCG 1 cut(s) 40
PvuII CAGCTG 1 cut(s) 830
RsaI GTAC 5 cut(s) 211, 718, 726, 842, 1209
RsaNI GTAC 5 cut(s) 210, 717, 725, 841, 1208
RseI CAYNNNNRTG 1 cut(s) 546
SapI GCTCTTC 1 cut(s) 745
SatI GCNGC 6 cut(s) 101, 138, 828, 942, 1227, 1428
Sau96I GGNCC 5 cut(s) 263, 420, 982, 1217, 1369
ScaI AGTACT 1 cut(s) 1209
SchI GAGTC 1 cut(s) 663
ScrFI CCNGG 3 cut(s) 13, 195, 729
SduI GDGCHC 1 cut(s) 1135
SfaNI GCATC 3 cut(s) 230, 385, 928
SfcI CTRYAG 2 cut(s) 831, 1621
SfuI TTCGAA 1 cut(s) 1611
SgrAI CRCCGGYG 1 cut(s) 764
SinI GGWCC 4 cut(s) 263, 420, 982, 1217
SmiMI CAYNNNNRTG 1 cut(s) 546
SmlI CTYRAG 3 cut(s) 586, 720, 1595
SmoI CTYRAG 3 cut(s) 586, 720, 1595
SnaBI TACGTA 1 cut(s) 1165
SsiI CCGC 3 cut(s) 352, 623, 1427
SspI AATATT 1 cut(s) 74
SspMI CTAG 1 cut(s) 1116
StyD4I CCNGG 3 cut(s) 11, 193, 727
TaaI ACNGT 2 cut(s) 185, 1510
TaiI ACGT 5 cut(s) 333, 446, 736, 1167, 1533
TaqI TCGA 5 cut(s) 40, 495, 603, 652, 1611
TatI WGTACW 2 cut(s) 840, 1207
TauI GCSGC 1 cut(s) 1430
TfiI GAWTC 4 cut(s) 405, 773, 920, 1172
TscAI CASTG 4 cut(s) 668, 787, 1513, 1583
TseFI GTSAC 3 cut(s) 529, 998, 1406
TseI GCWGC 5 cut(s) 100, 137, 827, 941, 1226
Tsp45I GTSAC 3 cut(s) 529, 998, 1406
TspGWI ACGGA 4 cut(s) 161, 339, 686, 1092
TspRI CASTG 4 cut(s) 668, 787, 1513, 1583
Van91I CCANNNNNTGG 1 cut(s) 61
Vha464I CTTAAG 2 cut(s) 586, 1595
VneI GTGCAC 1 cut(s) 1131
VpaK11BI GGWCC 4 cut(s) 263, 420, 982, 1217
XapI RAATTY 7 cut(s) 49, 167, 641, 1046, 1260, 1588, 1607
XmnI GAANNNNTTC 1 cut(s) 1607
XspI CTAG 1 cut(s) 1116
ZrmI AGTACT 1 cut(s) 1209
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.