MD10G1124500.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr10
OG0000117
Physical Location & Seq
Forward (+)
20354145 .. 20358614
4470 bp
Loading structure...
UTR
Exon/CDS
Intron
MD10G1124500.v1.1.491

Sequence Viewer

Length: 1443 bp
ATGGCAAAGTGCACCCTCCTTTTAGTCCTCTTGCTTGTTCTTGGTTGTGCATTGACAAGTAGTGGAAAATCTATCAAAACAGAGAACACTGGTGCTAAATCATGCGGTGAATCGTCTGCCTCCCTCAACCGGAGCAGCTTCCGAAGGGTTTCGTGTTCGGTGCAGCTTCATCGGCTTAAAGGTGCTGCAAATGAAGGTGGTAGAGGACCAAGTATATGGGATACATACATGCACAACCATCCAGAGAAGATAACTGACCGTAGCATTGGAGATGTGGCTGTTGATGAATATCATCGCTATAAGGAAGATGTTGAAATTATGAAGGACATTGGCCATGATGCTTACAGATTCTGGATCTCATGGTCTAGATTGTTACCAAATGGAAAGCTAAGCGGGGGTGTGAACTACGAAGGAATCAAATATTACAAGAACCTCATCGACGAACTTTTAGCCAATGGTCTAAAGCCCTTTGTCACAATCTTCCATTGGGATTTTCCTCAAGGTTTGGAGGATGATTACAGTGGTTTCTTAAGTCCTAAAGTTGACTATGCAGAGCTCTGCTTTAGAGAATTTGGTGACAAAGTTAAACATTGGATCACATTAAACGAGCCATTAAGCGTCAGCAAGAATGGTTATGCAAGCGGGAAGTATGCACCAGGCCGATGTTCTGACTGGCTAAACTTAAATTGCCTCAGTGGAGATTCCGGAACTGAACCATATTTGGTGACACACCAACAACTTCTAGCTCATGCAGCAGCTGTAAAGTTATATAGAGAAAAATATCAGGGGGTGATAGGGATAACATTGAACTCGGATTGGTATGTGCCAGTTTCTGAAGACAGGAACGAAAAAGATGCAGCTACAGGAGCATTGGATTTTTCATATGGATGGTATGTGTGCATACATATAGGGCCGGCCCTGAGAACTTGGGGCCCTAGGCGAGCCTTCGAAGTGGGGTTTATGGATCCCTTGGTGAAAGGTCATAATCCACACAGCATGCAAGCTCTTGTCGGAAATCGATTGCCAAATTTCACTAAAGAGCAATCCACGATGCTAAAAGGTTCGTTTCATTTTGTTGGACTAACTTATTATTCAGGAACTTATGCAGCCAATGTTCCTCGGCAAAATGATGCAAAACCAAACTACAAGACAGACGCTCTCGTTAATCAATCGGTCGAGTATAATGGTGTACCTATCGGTCCACTGGGTGCTTCTGATTGGCTCCATGTTTACCCGAGAGGAATTCGAGATTTATTGCTTTACACAAAGAGAAAGTATGATAATCCACTTATTTACATCACAGAAAATGGATATGACGACCCCAATGATCCCAACATACCTATCGAGGAATCCCCTGCTAATAAGAAAAGAATCGACTACCTTGATCGTCATATTTATTATATTCATAAAGCGATCGAGTCAGTAGCTAAGTGCATAGCCTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

481

Amino Acids

54.0

Weight (kDa)

7.24

Isoelectric Point (pI)

37.55

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 58 - 299 3.3e-78 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 320 - 474 1.1e-23 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccIII TCCGGA 1 cut(s) 704
AciI CCGC 3 cut(s) 105, 393, 642
AclWI GGATC 5 cut(s) 362, 602, 959, 972, 1322
AcoI YGGCCR 1 cut(s) 331
AcsI RAATTY 3 cut(s) 569, 1027, 1242
AcuI CTGAAG 1 cut(s) 855
AdeI CACNNNGTG 1 cut(s) 1208
AfaI GTAC 1 cut(s) 1191
AfiI CCNNNNNNNGG 1 cut(s) 129
AflII CTTAAG 1 cut(s) 529
AgsI TTSAA 2 cut(s) 314, 808
AjnI CCWGG 1 cut(s) 655
AluBI AGCT 9 cut(s) 138, 166, 388, 556, 746, 758, 860, 1004, 1427
AluI AGCT 9 cut(s) 138, 166, 388, 556, 746, 758, 860, 1004, 1427
Alw21I GWGCWC 2 cut(s) 14, 558
Alw44I GTGCAC 1 cut(s) 10
AlwI GGATC 5 cut(s) 362, 602, 959, 972, 1322
AlwNI CAGNNNCTG 3 cut(s) 351, 758, 833
Ama87I CYCGRG 1 cut(s) 1234
Aor13HI TCCGGA 1 cut(s) 704
AoxI GGCC 5 cut(s) 331, 658, 911, 915, 931
ApaI GGGCCC 1 cut(s) 935
ApaLI GTGCAC 1 cut(s) 10
ApeKI GCWGC 7 cut(s) 135, 163, 185, 752, 755, 857, 1106
ApoI RAATTY 3 cut(s) 569, 1027, 1242
Asp700I GAANNNNTTC 1 cut(s) 148
AspA2I CCTAGG 1 cut(s) 935
AspS9I GGNCC 6 cut(s) 206, 911, 916, 931, 932, 1199
AsuHPI GGTGA 5 cut(s) 119, 587, 736, 802, 985
AsuII TTCGAA 1 cut(s) 948
AvaI CYCGRG 1 cut(s) 1234
AvaII GGWCC 2 cut(s) 206, 1199
AvrII CCTAGG 1 cut(s) 935
BaeGI GKGCMC 2 cut(s) 14, 935
BalI TGGCCA 1 cut(s) 333
BamHI GGATCC 1 cut(s) 964
BanII GRGCYC 2 cut(s) 558, 935
BbsI GAAGAC 1 cut(s) 843
Bbv12I GWGCWC 2 cut(s) 14, 558
BbvI GCAGC 7 cut(s) 147, 172, 175, 764, 767, 869, 1118
BccI CCATC 2 cut(s) 246, 882
BcgI CGANNNNNNTGC 4 cut(s) 152, 186, 836, 870
BciT130I CCWGG 1 cut(s) 657
BciVI GTATCC 1 cut(s) 214
BfaI CTAG 3 cut(s) 366, 743, 936
BfmI CTRYAG 1 cut(s) 861
BfrI CTTAAG 1 cut(s) 529
BfuI GTATCC 1 cut(s) 214
BisI GCNGC 7 cut(s) 136, 164, 186, 753, 756, 858, 1107
BlnI CCTAGG 1 cut(s) 935
BlpI GCTNAGC 1 cut(s) 389
BlsI GCNGC 7 cut(s) 137, 165, 187, 754, 757, 859, 1108
Bme1390I CCNGG 1 cut(s) 657
Bme18I GGWCC 2 cut(s) 206, 1199
BmeT110I CYCGRG 1 cut(s) 1234
BmgT120I GGNCC 6 cut(s) 206, 911, 916, 931, 932, 1199
BmiI GGNNCC 4 cut(s) 932, 933, 966, 1223
BmrFI CCNGG 1 cut(s) 657
BmrI ACTGGG 1 cut(s) 1214
BmsI GCATC 4 cut(s) 328, 844, 1041, 1120
BmuI ACTGGG 1 cut(s) 1214
BpiI GAAGAC 1 cut(s) 843
Bpu1102I GCTNAGC 1 cut(s) 389
Bpu14I TTCGAA 1 cut(s) 948
BpuEI CTTGAG 1 cut(s) 483
Bsa29I ATCGAT 1 cut(s) 1018
BsaBI GATNNNNATC 1 cut(s) 288
BsaJI CCNNGG 3 cut(s) 935, 969, 1118
BsaWI WCCGGW 2 cut(s) 129, 704
Bsc4I CCNNNNNNNGG 1 cut(s) 129
Bse118I RCCGGY 1 cut(s) 913
Bse1I ACTGG 4 cut(s) 94, 677, 827, 1209
Bse8I GATNNNNATC 1 cut(s) 288
BseAI TCCGGA 1 cut(s) 704
BseBI CCWGG 1 cut(s) 657
BseCI ATCGAT 1 cut(s) 1018
BseDI CCNNGG 3 cut(s) 935, 969, 1118
BseGI GGATG 3 cut(s) 238, 517, 893
BseJI GATNNNNATC 1 cut(s) 288
BseLI CCNNNNNNNGG 1 cut(s) 129
BseMII CTCAG 2 cut(s) 706, 911
BseNI ACTGG 4 cut(s) 94, 677, 827, 1209
BseSI GKGCMC 2 cut(s) 14, 935
BseXI GCAGC 7 cut(s) 147, 172, 175, 764, 767, 869, 1118
BsgI GTGCAG 1 cut(s) 182
Bsh1285I CGRYCG 2 cut(s) 1176, 1416
BshFI GGCC 5 cut(s) 333, 660, 913, 917, 933
BshVI ATCGAT 1 cut(s) 1018
BsiEI CGRYCG 2 cut(s) 1176, 1416
BsiHKAI GWGCWC 2 cut(s) 14, 558
BsiHKCI CYCGRG 1 cut(s) 1234
BsiSI CCGG 3 cut(s) 130, 705, 914
BslI CCNNNNNNNGG 1 cut(s) 129
BsnI GGCC 5 cut(s) 333, 660, 913, 917, 933
BsoBI CYCGRG 1 cut(s) 1234
Bsp119I TTCGAA 1 cut(s) 948
Bsp120I GGGCCC 1 cut(s) 931
Bsp1286I GDGCHC 3 cut(s) 14, 558, 935
Bsp13I TCCGGA 1 cut(s) 704
Bsp143I GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
Bsp1720I GCTNAGC 1 cut(s) 389
BspACI CCGC 3 cut(s) 105, 393, 642
BspANI GGCC 5 cut(s) 333, 660, 913, 917, 933
BspCNI CTCAG 2 cut(s) 705, 912
BspDI ATCGAT 1 cut(s) 1018
BspEI TCCGGA 1 cut(s) 704
BspLI GGNNCC 4 cut(s) 932, 933, 966, 1223
BspPI GGATC 5 cut(s) 362, 602, 959, 972, 1322
BspT104I TTCGAA 1 cut(s) 948
BspTI CTTAAG 1 cut(s) 529
BsrFI RCCGGY 1 cut(s) 913
BsrI ACTGG 4 cut(s) 94, 677, 827, 1209
BssAI RCCGGY 1 cut(s) 913
BssECI CCNNGG 3 cut(s) 935, 969, 1118
BssMI GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
BssT1I CCWWGG 2 cut(s) 935, 969
Bst2UI CCWGG 1 cut(s) 657
Bst4CI ACNGT 2 cut(s) 260, 521
BstAFI CTTAAG 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 948
BstC8I GCNNGC 5 cut(s) 640, 915, 942, 998, 1002
BstDEI CTNAG 4 cut(s) 389, 692, 920, 1428
BstF5I GGATG 3 cut(s) 238, 517, 893
BstKTI GATC 6 cut(s) 357, 597, 967, 1330, 1387, 1416
BstMBI GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
BstMCI CGRYCG 2 cut(s) 1176, 1416
BstMWI GCNNNNNNNGC 3 cut(s) 172, 752, 866
BstNI CCWGG 1 cut(s) 657
BstNSI RCATGY 2 cut(s) 232, 1000
BstSCI CCNGG 1 cut(s) 655
BstSFI CTRYAG 1 cut(s) 861
BstSLI GKGCMC 2 cut(s) 14, 935
BstV1I GCAGC 7 cut(s) 147, 172, 175, 764, 767, 869, 1118
BstV2I GAAGAC 1 cut(s) 843
BstX2I RGATCY 2 cut(s) 354, 964
BstXI CCANNNNNNTGG 1 cut(s) 216
BstYI RGATCY 2 cut(s) 354, 964
Bsu15I ATCGAT 1 cut(s) 1018
BsuI GTATCC 1 cut(s) 214
BsuRI GGCC 5 cut(s) 333, 660, 913, 917, 933
BsuTUI ATCGAT 1 cut(s) 1018
BtgZI GCGATG 1 cut(s) 278
BtsCI GGATG 3 cut(s) 238, 517, 893
BtsIMutI CAGTG 4 cut(s) 87, 526, 700, 1202
Cac8I GCNNGC 5 cut(s) 640, 915, 942, 998, 1002
CaiI CAGNNNCTG 3 cut(s) 351, 758, 833
Cfr10I RCCGGY 1 cut(s) 913
Cfr13I GGNCC 6 cut(s) 206, 911, 916, 931, 932, 1199
ClaI ATCGAT 1 cut(s) 1018
CseI GACGC 2 cut(s) 607, 1163
Csp6I GTAC 1 cut(s) 1190
CviAII CATG 7 cut(s) 102, 229, 335, 360, 749, 997, 1226
CviQI GTAC 1 cut(s) 1190
DdeI CTNAG 4 cut(s) 389, 692, 920, 1428
DpnI GATC 6 cut(s) 356, 596, 966, 1329, 1386, 1415
DpnII GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
DraIII CACNNNGTG 1 cut(s) 1208
EaeI YGGCCR 1 cut(s) 331
Ecl136II GAGCTC 1 cut(s) 556
Eco130I CCWWGG 2 cut(s) 935, 969
Eco24I GRGCYC 2 cut(s) 558, 935
Eco47I GGWCC 2 cut(s) 206, 1199
Eco53kI GAGCTC 1 cut(s) 556
Eco57I CTGAAG 1 cut(s) 855
Eco88I CYCGRG 1 cut(s) 1234
EcoICRI GAGCTC 1 cut(s) 556
EcoO109I RGGNCCY 2 cut(s) 931, 932
EcoRI GAATTC 1 cut(s) 1242
EcoRII CCWGG 1 cut(s) 655
EcoT14I CCWWGG 2 cut(s) 935, 969
EcoT38I GRGCYC 2 cut(s) 558, 935
ErhI CCWWGG 2 cut(s) 935, 969
FaeI CATG 7 cut(s) 105, 232, 338, 363, 752, 1000, 1229
FatI CATG 7 cut(s) 101, 228, 334, 359, 748, 996, 1225
FauI CCCGC 2 cut(s) 386, 635
FauNDI CATATG 1 cut(s) 883
Fnu4HI GCNGC 7 cut(s) 136, 164, 186, 753, 756, 858, 1107
FokI GGATG 3 cut(s) 225, 524, 900
FriOI GRGCYC 2 cut(s) 558, 935
FseI GGCCGGCC 1 cut(s) 917
Fsp4HI GCNGC 7 cut(s) 136, 164, 186, 753, 756, 858, 1107
FspBI CTAG 3 cut(s) 366, 743, 936
GluI GCNGC 7 cut(s) 136, 164, 186, 753, 756, 858, 1107
HaeIII GGCC 5 cut(s) 333, 660, 913, 917, 933
HapII CCGG 3 cut(s) 130, 705, 914
HgaI GACGC 2 cut(s) 607, 1163
Hin1II CATG 7 cut(s) 105, 232, 338, 363, 752, 1000, 1229
HincII GTYRAC 1 cut(s) 544
HindII GTYRAC 1 cut(s) 544
HinfI GANTC 7 cut(s) 110, 348, 414, 701, 1349, 1371, 1418
HpaII CCGG 3 cut(s) 130, 705, 914
HphI GGTGA 5 cut(s) 119, 587, 736, 802, 985
Hpy166II GTNNAC 6 cut(s) 12, 403, 544, 1190, 1202, 1231
Hpy188I TCNGA 6 cut(s) 143, 670, 814, 835, 1013, 1216
Hpy188III TCNNGA 6 cut(s) 242, 352, 366, 705, 1095, 1247
Hpy8I GTNNAC 6 cut(s) 12, 403, 544, 1190, 1202, 1231
Hpy99I CGWCG 1 cut(s) 443
HpyAV CCTTC 5 cut(s) 138, 188, 316, 404, 955
HpyCH4III ACNGT 2 cut(s) 260, 521
HpyF10VI GCNNNNNNNGC 3 cut(s) 172, 752, 866
HpyF3I CTNAG 4 cut(s) 389, 692, 920, 1428
Hsp92II CATG 7 cut(s) 105, 232, 338, 363, 752, 1000, 1229
Kpn2I TCCGGA 1 cut(s) 704
KroI GCCGGC 1 cut(s) 913
KroNI GCCGGC 1 cut(s) 915
Kzo9I GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
LmnI GCTCC 3 cut(s) 132, 866, 1227
Lsp1109I GCAGC 7 cut(s) 147, 172, 175, 764, 767, 869, 1118
LweI GCATC 4 cut(s) 328, 844, 1041, 1120
MaeI CTAG 3 cut(s) 366, 743, 936
MaeIII GTNAC 4 cut(s) 372, 472, 575, 724
MalI GATC 6 cut(s) 356, 596, 966, 1329, 1386, 1415
MboI GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
MboII GAAGA 4 cut(s) 259, 317, 472, 848
MflI RGATCY 2 cut(s) 354, 964
MhlI GDGCHC 3 cut(s) 14, 558, 935
MlsI TGGCCA 1 cut(s) 333
MluCI AATT 5 cut(s) 315, 569, 685, 1027, 1242
MluNI TGGCCA 1 cut(s) 333
MlyI GAGTC 1 cut(s) 1427
MmeI TCCRAC 2 cut(s) 991, 1057
Mox20I TGGCCA 1 cut(s) 333
MroI TCCGGA 1 cut(s) 704
MroNI GCCGGC 1 cut(s) 913
MroXI GAANNNNTTC 1 cut(s) 148
MscI TGGCCA 1 cut(s) 333
MseI TTAA 7 cut(s) 177, 530, 585, 602, 614, 683, 1164
MslI CAYNNNNRTG 1 cut(s) 886
Msp20I TGGCCA 1 cut(s) 333
MspA1I CMGCKG 1 cut(s) 758
MspCI CTTAAG 1 cut(s) 529
MspI CCGG 3 cut(s) 130, 705, 914
MspR9I CCNGG 1 cut(s) 657
MvaI CCWGG 1 cut(s) 657
MwoI GCNNNNNNNGC 3 cut(s) 172, 752, 866
NaeI GCCGGC 1 cut(s) 915
NdeI CATATG 1 cut(s) 883
NdeII GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
NgoMIV GCCGGC 1 cut(s) 913
NlaIII CATG 7 cut(s) 105, 232, 338, 363, 752, 1000, 1229
NlaIV GGNNCC 4 cut(s) 932, 933, 966, 1223
NmeAIII GCCGAG 1 cut(s) 1099
NmuCI GTSAC 3 cut(s) 472, 575, 724
NspI RCATGY 2 cut(s) 232, 1000
NspV TTCGAA 1 cut(s) 948
PaeI GCATGC 1 cut(s) 1000
PdiI GCCGGC 1 cut(s) 915
PdmI GAANNNNTTC 1 cut(s) 148
PfeI GAWTC 6 cut(s) 110, 348, 414, 701, 1349, 1371
PkrI GCNGC 7 cut(s) 137, 165, 187, 754, 757, 859, 1108
Ple19I CGATCG 1 cut(s) 1416
PleI GAGTC 1 cut(s) 1426
PpsI GAGTC 1 cut(s) 1426
Psp124BI GAGCTC 1 cut(s) 558
Psp6I CCWGG 1 cut(s) 655
PspGI CCWGG 1 cut(s) 655
PspN4I GGNNCC 4 cut(s) 932, 933, 966, 1223
PspOMI GGGCCC 1 cut(s) 931
PspPI GGNCC 6 cut(s) 206, 911, 916, 931, 932, 1199
PstNI CAGNNNCTG 3 cut(s) 351, 758, 833
PsuI RGATCY 2 cut(s) 354, 964
PvuI CGATCG 1 cut(s) 1416
PvuII CAGCTG 1 cut(s) 758
RigI GGCCGGCC 1 cut(s) 917
RsaI GTAC 1 cut(s) 1191
RsaNI GTAC 1 cut(s) 1190
RseI CAYNNNNRTG 1 cut(s) 886
SacI GAGCTC 1 cut(s) 558
SaqAI TTAA 7 cut(s) 177, 530, 585, 602, 614, 683, 1164
SatI GCNGC 7 cut(s) 136, 164, 186, 753, 756, 858, 1107
Sau3AI GATC 6 cut(s) 354, 594, 964, 1327, 1384, 1413
Sau96I GGNCC 6 cut(s) 206, 911, 916, 931, 932, 1199
SchI GAGTC 1 cut(s) 1427
ScrFI CCNGG 1 cut(s) 657
SduI GDGCHC 3 cut(s) 14, 558, 935
SfaNI GCATC 4 cut(s) 328, 844, 1041, 1120
SfcI CTRYAG 1 cut(s) 861
SfuI TTCGAA 1 cut(s) 948
SinI GGWCC 2 cut(s) 206, 1199
SmiMI CAYNNNNRTG 1 cut(s) 886
SmlI CTYRAG 2 cut(s) 498, 529
SmoI CTYRAG 2 cut(s) 498, 529
SphI GCATGC 1 cut(s) 1000
Sse9I AATT 5 cut(s) 315, 569, 685, 1027, 1242
SsiI CCGC 3 cut(s) 105, 393, 642
SspI AATATT 1 cut(s) 422
SspMI CTAG 3 cut(s) 366, 743, 936
SstI GAGCTC 1 cut(s) 558
StyD4I CCNGG 1 cut(s) 655
StyI CCWWGG 2 cut(s) 935, 969
TaaI ACNGT 2 cut(s) 260, 521
TaqI TCGA 8 cut(s) 438, 948, 1018, 1176, 1246, 1344, 1374, 1416
TaqII GACCGA 2 cut(s) 1162, 1187
TasI AATT 5 cut(s) 315, 569, 685, 1027, 1242
TfiI GAWTC 6 cut(s) 110, 348, 414, 701, 1349, 1371
Tru1I TTAA 7 cut(s) 177, 530, 585, 602, 614, 683, 1164
Tru9I TTAA 7 cut(s) 177, 530, 585, 602, 614, 683, 1164
TscAI CASTG 4 cut(s) 94, 526, 700, 1209
TseFI GTSAC 3 cut(s) 472, 575, 724
TseI GCWGC 7 cut(s) 135, 163, 185, 752, 755, 857, 1106
Tsp45I GTSAC 3 cut(s) 472, 575, 724
TspDTI ATGAA 7 cut(s) 158, 207, 300, 335, 870, 1058, 1394
TspRI CASTG 4 cut(s) 94, 526, 700, 1209
Vha464I CTTAAG 1 cut(s) 529
VneI GTGCAC 1 cut(s) 10
VpaK11BI GGWCC 2 cut(s) 206, 1199
XapI RAATTY 3 cut(s) 569, 1027, 1242
XbaI TCTAGA 1 cut(s) 365
XceI RCATGY 2 cut(s) 232, 1000
XmaJI CCTAGG 1 cut(s) 935
XmnI GAANNNNTTC 1 cut(s) 148
XspI CTAG 3 cut(s) 366, 743, 936
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.