MD00G1105700.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0000117
Physical Location & Seq
Forward (+)
22051599 .. 22055203
3605 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1105700.v1.1.491

Sequence Viewer

Length: 1617 bp
ATGGCAATGCATGAGTTAGGCTCTTTGCTCTTGTGTGTCTTGCTACTCAATGGCTTTGCATTGACAAACACCAAAGCTGCTAACCCAGATCGACCCATTGTCTGTAACTCACTTGACAGGACCAAGTTTGATGCTCTAAAACCAGGGTTCGTCTTTGGTGCAGCTTCAGCAGCTTACCAGGTAGAAGGTGCATGGAACGAAGATGGTAGAGGACCAAGCATATGGGACACCTTCACCCACAACCATCCAGAAAAAATCACTGATCGCAGCAATGGAGATGTCGCCATTGATCAATACCACCTCTATAAGAAAGATGTAGCAATTATGAAGGATATGAAGTTGGATGCTTATAGGTTCTCTATCTCATGGCCCAGATTGTTACCAAATGGCACGCTAAGTGGGGGTGTCAACAGGAAAGGAATTGAATATTACGACAATCTCATCAATGAACTCCTTCGCAATGGCATACAACCATTTGTGACAATCTTTCACTGGGATGTTCCCCAAGCGTTAGAAGATGCATATGGTGGTTTCTTAAGCGCTAGTATTGTCGATGACTTTAAAGACTACGCAGAACTTTGTTTTTCACTTTTTGGTGATCGGGTGAAGCACTGGATCACGTTGAATGAGCCATATACCTTCAGTAACCATGCATATACAATCGGGATCCACGCACCGGGACGATGCTCCGCTTGGCAAGACCCAACCTGCCTCGGTGGAGATTCGGCTACTGAACCCTATTTGGTAACACACCACCAACTCCTTGCTCATGCAGCTGCTGTAAAAGTGTACAAGGATAAATTTCAGGCATATCAAAATGGGGTGATAGGAATAACACTAGTGTCACATTGGTATGAGCCTGCTTCAGATGCAAAGGAAGATATAGATGCTGCAAATCGAGCTTTGGATTTTATGTTTGGATGGTTTATGGATCCAATTACAAGAGGTGACTACCCGTACAACATGCGATGCCTTGTTAGAGAACGATTGCCAAAATTCACGGAAGAGGAATCCAAGATGTTAACTGGGTCTTTTGATTTTGTTGGATTGAACTATTATTCTGCTAGATATGCAACTGATGTACCTAAGAATTATTCTAAACCTGCAAGTTACTTATACGATCCACATGTTACTACACTGACTGAACGTGAGGGCATTCCTATTGGTCCTCAGGCTGCTTCAGATTGGTTATATGTTTATCCAAAAGGAATTCACGATTTTGTTCTCTACACGAAGAATAAGTATCATGATCCAATCATTTACATTACTGAGAATGGCGTTGATGAGGTCAATAATTCCACCTTATCACTCGATGATGCCCTCTATGATACCAATAGGACTGACTACTACAATCGCCACCTCTGTTACCTTCAAGCAGCAATCAAGAAGGGTAGTAATGTGAAAGGATACTTTGCATGGTCAATTTTAGACAACTTTGAATGGAGTGAAGGCTACACAGTTCGATTTGGTATTAACTATGTGGATTATGACAATGGACTCCAAAGGTACCCAAAACTTTCGACCTATTGGTTCAAAAATTTCCTCAAGAAGCGCAAAGGAAGTTCAAATATTTTGGCCAATAATGTTGGAGACACTGATTTTCTCTATCAGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

539

Amino Acids

61.34

Weight (kDa)

5.67

Isoelectric Point (pI)

21.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 46 - 518 3.5e-162 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 716, 1111
Acc65I GGTACC 1 cut(s) 1506
AccB1I GGYRCC 1 cut(s) 1506
AciI CCGC 1 cut(s) 690
AclWI GGATC 7 cut(s) 623, 661, 674, 926, 939, 1115, 1244
AcoI YGGCCR 1 cut(s) 1575
AcsI RAATTY 4 cut(s) 800, 995, 1209, 1537
AcuI CTGAAG 4 cut(s) 150, 625, 849, 1164
AfaI GTAC 4 cut(s) 791, 959, 1083, 1508
AfeI AGCGCT 1 cut(s) 541
AfiI CCNNNNNNNGG 1 cut(s) 676
AflII CTTAAG 1 cut(s) 535
AflIII ACRYGT 1 cut(s) 1126
AgsI TTSAA 7 cut(s) 425, 625, 1051, 1373, 1439, 1534, 1566
AhdI GACNNNNNGTC 1 cut(s) 98
AhlI ACTAGT 1 cut(s) 838
AjnI CCWGG 2 cut(s) 142, 177
AluBI AGCT 5 cut(s) 77, 164, 173, 776, 902
AluI AGCT 5 cut(s) 77, 164, 173, 776, 902
Alw26I GTCTC 1 cut(s) 1584
AlwI GGATC 7 cut(s) 623, 661, 674, 926, 939, 1115, 1244
AlwNI CAGNNNCTG 1 cut(s) 779
Aor51HI AGCGCT 1 cut(s) 541
AoxI GGCC 2 cut(s) 368, 1575
ApeKI GCWGC 9 cut(s) 77, 161, 170, 267, 773, 776, 890, 1175, 1376
ApoI RAATTY 4 cut(s) 800, 995, 1209, 1537
Asp700I GAANNNNTTC 1 cut(s) 453
Asp718I GGTACC 1 cut(s) 1506
AspLEI GCGC 2 cut(s) 542, 1554
AspS9I GGNCC 4 cut(s) 120, 212, 369, 1166
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 5 cut(s) 226, 608, 616, 835, 959
AvaII GGWCC 3 cut(s) 120, 212, 1166
AxyI CCTNAGG 1 cut(s) 1170
BaeI ACNNNNGTAYC 2 cut(s) 1065, 1098
BalI TGGCCA 1 cut(s) 1577
BamHI GGATCC 2 cut(s) 666, 931
BanI GGYRCC 1 cut(s) 1506
BbvI GCAGC 9 cut(s) 64, 173, 182, 279, 763, 785, 877, 1162, 1388
BccI CCATC 3 cut(s) 197, 252, 915
BciT130I CCWGG 2 cut(s) 144, 179
BciVI GTATCC 1 cut(s) 1400
BclI TGATCA 1 cut(s) 289
BcnI CCSGG 1 cut(s) 678
BcoDI GTCTC 1 cut(s) 1584
BcuI ACTAGT 1 cut(s) 838
BfaI CTAG 3 cut(s) 543, 839, 1065
BfoI RGCGCY 1 cut(s) 543
BfrI CTTAAG 1 cut(s) 535
BfuAI ACCTGC 2 cut(s) 716, 1111
BfuI GTATCC 1 cut(s) 1400
BisI GCNGC 9 cut(s) 78, 162, 171, 268, 774, 777, 891, 1176, 1377
BlsI GCNGC 9 cut(s) 79, 163, 172, 269, 775, 778, 892, 1177, 1378
Bme1390I CCNGG 3 cut(s) 144, 179, 678
Bme18I GGWCC 3 cut(s) 120, 212, 1166
BmeRI GACNNNNNGTC 1 cut(s) 98
BmgT120I GGNCC 4 cut(s) 120, 212, 369, 1166
BmiI GGNNCC 3 cut(s) 668, 933, 1508
BmrFI CCNGG 3 cut(s) 144, 179, 678
BmrI ACTGGG 2 cut(s) 502, 1035
BmsI GCATC 8 cut(s) 121, 334, 508, 674, 859, 877, 959, 1306
BmuI ACTGGG 2 cut(s) 502, 1035
BplI GAGNNNNNCTC 1 cut(s) 37
BpuEI CTTGAG 1 cut(s) 1529
BpuMI CCSGG 1 cut(s) 678
BsaJI CCNNGG 2 cut(s) 143, 712
Bsc4I CCNNNNNNNGG 1 cut(s) 676
Bse1I ACTGG 3 cut(s) 497, 617, 1030
Bse21I CCTNAGG 1 cut(s) 1170
Bse3DI GCAATG 3 cut(s) 12, 277, 466
BseBI CCWGG 2 cut(s) 144, 179
BseDI CCNNGG 2 cut(s) 143, 712
BseGI GGATG 4 cut(s) 244, 349, 502, 926
BseLI CCNNNNNNNGG 1 cut(s) 676
BseMI GCAATG 3 cut(s) 12, 277, 466
BseMII CTCAG 2 cut(s) 1184, 1260
BseNI ACTGG 3 cut(s) 497, 617, 1030
BseXI GCAGC 9 cut(s) 64, 173, 182, 279, 763, 785, 877, 1162, 1388
BsgI GTGCAG 1 cut(s) 180
BshFI GGCC 2 cut(s) 370, 1577
BshNI GGYRCC 1 cut(s) 1506
BsiSI CCGG 1 cut(s) 677
BslFI GGGAC 2 cut(s) 239, 693
BslI CCNNNNNNNGG 1 cut(s) 676
BsmAI GTCTC 1 cut(s) 1584
BsmFI GGGAC 2 cut(s) 239, 693
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 2 cut(s) 370, 1577
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
BspACI CCGC 1 cut(s) 690
BspANI GGCC 2 cut(s) 370, 1577
BspCNI CTCAG 2 cut(s) 1183, 1261
BspHI TCATGA 1 cut(s) 1246
BspLI GGNNCC 3 cut(s) 668, 933, 1508
BspMI ACCTGC 2 cut(s) 716, 1111
BspPI GGATC 7 cut(s) 623, 661, 674, 926, 939, 1115, 1244
BspT107I GGYRCC 1 cut(s) 1506
BspTI CTTAAG 1 cut(s) 535
BsrDI GCAATG 3 cut(s) 12, 277, 466
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 3 cut(s) 497, 617, 1030
BssECI CCNNGG 2 cut(s) 143, 712
BssMI GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
Bst2UI CCWGG 2 cut(s) 144, 179
Bst4CI ACNGT 1 cut(s) 1459
Bst6I CTCTTC 1 cut(s) 999
BstAFI CTTAAG 1 cut(s) 535
BstAUI TGTACA 1 cut(s) 789
BstC8I GCNNGC 2 cut(s) 392, 861
BstDEI CTNAG 4 cut(s) 395, 1086, 1170, 1269
BstF5I GGATG 4 cut(s) 244, 349, 502, 926
BstH2I RGCGCY 1 cut(s) 543
BstHHI GCGC 2 cut(s) 542, 1554
BstKTI GATC 9 cut(s) 91, 265, 292, 601, 618, 669, 934, 1123, 1252
BstMAI GTCTC 1 cut(s) 1584
BstMBI GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
BstMWI GCNNNNNNNGC 6 cut(s) 167, 170, 773, 869, 899, 1070
BstNI CCWGG 2 cut(s) 144, 179
BstNSI RCATGY 2 cut(s) 967, 1130
BstSCI CCNGG 3 cut(s) 142, 177, 676
BstV1I GCAGC 9 cut(s) 64, 173, 182, 279, 763, 785, 877, 1162, 1388
BstX2I RGATCY 2 cut(s) 666, 931
BstXI CCANNNNNNTGG 1 cut(s) 222
BstYI RGATCY 2 cut(s) 666, 931
Bsu36I CCTNAGG 1 cut(s) 1170
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 2 cut(s) 370, 1577
BtgZI GCGATG 1 cut(s) 982
BtsCI GGATG 4 cut(s) 244, 349, 502, 926
BtsIMutI CAGTG 5 cut(s) 258, 490, 610, 1136, 1593
BveI ACCTGC 2 cut(s) 716, 1111
Cac8I GCNNGC 2 cut(s) 392, 861
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 1 cut(s) 1246
CfoI GCGC 2 cut(s) 542, 1554
Cfr13I GGNCC 4 cut(s) 120, 212, 369, 1166
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 4 cut(s) 790, 958, 1082, 1507
CviAII CATG 9 cut(s) 11, 192, 366, 650, 770, 964, 1127, 1247, 1416
CviQI GTAC 4 cut(s) 790, 958, 1082, 1507
DdeI CTNAG 4 cut(s) 395, 1086, 1170, 1269
DpnI GATC 9 cut(s) 90, 264, 291, 600, 617, 668, 933, 1122, 1251
DpnII GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
DraI TTTAAA 1 cut(s) 562
DriI GACNNNNNGTC 1 cut(s) 98
EaeI YGGCCR 1 cut(s) 1575
Eam1104I CTCTTC 1 cut(s) 999
Eam1105I GACNNNNNGTC 1 cut(s) 98
EarI CTCTTC 1 cut(s) 999
Eco47I GGWCC 3 cut(s) 120, 212, 1166
Eco47III AGCGCT 1 cut(s) 541
Eco57I CTGAAG 4 cut(s) 150, 625, 849, 1164
Eco81I CCTNAGG 1 cut(s) 1170
EcoRI GAATTC 1 cut(s) 1209
EcoRII CCWGG 2 cut(s) 142, 177
EcoT22I ATGCAT 3 cut(s) 12, 523, 655
FaeI CATG 9 cut(s) 14, 195, 369, 653, 773, 967, 1130, 1250, 1419
FaqI GGGAC 2 cut(s) 239, 693
FatI CATG 9 cut(s) 10, 191, 365, 649, 769, 963, 1126, 1246, 1415
FauNDI CATATG 2 cut(s) 221, 523
FbaI TGATCA 1 cut(s) 289
Fnu4HI GCNGC 9 cut(s) 78, 162, 171, 268, 774, 777, 891, 1176, 1377
FokI GGATG 4 cut(s) 231, 356, 509, 933
Fsp4HI GCNGC 9 cut(s) 78, 162, 171, 268, 774, 777, 891, 1176, 1377
FspBI CTAG 3 cut(s) 543, 839, 1065
GlaI GCGC 2 cut(s) 541, 1553
GluI GCNGC 9 cut(s) 78, 162, 171, 268, 774, 777, 891, 1176, 1377
HaeII RGCGCY 1 cut(s) 543
HaeIII GGCC 2 cut(s) 370, 1577
HapII CCGG 1 cut(s) 677
HhaI GCGC 2 cut(s) 542, 1554
Hin1II CATG 9 cut(s) 14, 195, 369, 653, 773, 967, 1130, 1250, 1419
Hin6I GCGC 2 cut(s) 540, 1552
HinP1I GCGC 2 cut(s) 540, 1552
HincII GTYRAC 2 cut(s) 409, 1023
HindII GTYRAC 2 cut(s) 409, 1023
HinfI GANTC 3 cut(s) 722, 1010, 1497
HpaI GTTAAC 1 cut(s) 1023
HpaII CCGG 1 cut(s) 677
HphI GGTGA 5 cut(s) 226, 608, 616, 835, 959
Hpy166II GTNNAC 3 cut(s) 409, 790, 1023
Hpy188I TCNGA 2 cut(s) 868, 1183
Hpy188III TCNNGA 6 cut(s) 248, 664, 1214, 1247, 1384, 1546
Hpy8I GTNNAC 3 cut(s) 409, 790, 1023
HpyAV CCTTC 8 cut(s) 179, 241, 322, 464, 649, 1379, 1381, 1442
HpyCH4III ACNGT 1 cut(s) 1459
HpyCH4IV ACGT 2 cut(s) 620, 1147
HpyF10VI GCNNNNNNNGC 6 cut(s) 167, 170, 773, 869, 899, 1070
HpyF3I CTNAG 4 cut(s) 395, 1086, 1170, 1269
HpySE526I ACGT 2 cut(s) 620, 1147
Hsp92II CATG 9 cut(s) 14, 195, 369, 653, 773, 967, 1130, 1250, 1419
HspAI GCGC 2 cut(s) 540, 1552
KpnI GGTACC 1 cut(s) 1510
Ksp22I TGATCA 1 cut(s) 289
KspAI GTTAAC 1 cut(s) 1023
Kzo9I GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
LmnI GCTCC 1 cut(s) 692
Lsp1109I GCAGC 9 cut(s) 64, 173, 182, 279, 763, 785, 877, 1162, 1388
LweI GCATC 8 cut(s) 121, 334, 508, 674, 859, 877, 959, 1306
MabI ACCWGGT 1 cut(s) 177
MaeI CTAG 3 cut(s) 543, 839, 1065
MaeII ACGT 2 cut(s) 620, 1147
MalI GATC 9 cut(s) 90, 264, 291, 600, 617, 668, 933, 1122, 1251
MboI GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
MboII GAAGA 5 cut(s) 212, 527, 890, 1016, 1246
MflI RGATCY 2 cut(s) 666, 931
MlsI TGGCCA 1 cut(s) 1577
MluNI TGGCCA 1 cut(s) 1577
MlyI GAGTC 1 cut(s) 1491
MmeI TCCRAC 3 cut(s) 321, 1024, 1567
Mox20I TGGCCA 1 cut(s) 1577
Mph1103I ATGCAT 3 cut(s) 12, 523, 655
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 1 cut(s) 1577
MseI TTAA 4 cut(s) 536, 561, 1022, 1473
MslI CAYNNNNRTG 2 cut(s) 495, 852
Msp20I TGGCCA 1 cut(s) 1577
MspA1I CMGCKG 1 cut(s) 776
MspCI CTTAAG 1 cut(s) 535
MspI CCGG 1 cut(s) 677
MspR9I CCNGG 3 cut(s) 144, 179, 678
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 2 cut(s) 144, 179
MwoI GCNNNNNNNGC 6 cut(s) 167, 170, 773, 869, 899, 1070
NciI CCSGG 1 cut(s) 678
NdeI CATATG 2 cut(s) 221, 523
NdeII GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
NlaIII CATG 9 cut(s) 14, 195, 369, 653, 773, 967, 1130, 1250, 1419
NlaIV GGNNCC 3 cut(s) 668, 933, 1508
NmuCI GTSAC 3 cut(s) 478, 843, 947
NsiI ATGCAT 3 cut(s) 12, 523, 655
NspI RCATGY 2 cut(s) 967, 1130
PagI TCATGA 1 cut(s) 1246
PciI ACATGT 1 cut(s) 1126
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 2 cut(s) 722, 1010
PkrI GCNGC 9 cut(s) 79, 163, 172, 269, 775, 778, 892, 1177, 1378
PleI GAGTC 1 cut(s) 1491
PpsI GAGTC 1 cut(s) 1491
PscI ACATGT 1 cut(s) 1126
Psp6I CCWGG 2 cut(s) 142, 177
PspGI CCWGG 2 cut(s) 142, 177
PspN4I GGNNCC 3 cut(s) 668, 933, 1508
PspPI GGNCC 4 cut(s) 120, 212, 369, 1166
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 2 cut(s) 666, 931
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 4 cut(s) 791, 959, 1083, 1508
RsaNI GTAC 4 cut(s) 790, 958, 1082, 1507
RseI CAYNNNNRTG 2 cut(s) 495, 852
SaqAI TTAA 4 cut(s) 536, 561, 1022, 1473
SatI GCNGC 9 cut(s) 78, 162, 171, 268, 774, 777, 891, 1176, 1377
Sau3AI GATC 9 cut(s) 88, 262, 289, 598, 615, 666, 931, 1120, 1249
Sau96I GGNCC 4 cut(s) 120, 212, 369, 1166
SchI GAGTC 1 cut(s) 1491
ScrFI CCNGG 3 cut(s) 144, 179, 678
SexAI ACCWGGT 1 cut(s) 177
SfaNI GCATC 8 cut(s) 121, 334, 508, 674, 859, 877, 959, 1306
SinI GGWCC 3 cut(s) 120, 212, 1166
SmiMI CAYNNNNRTG 2 cut(s) 495, 852
SmlI CTYRAG 2 cut(s) 535, 1544
SmoI CTYRAG 2 cut(s) 535, 1544
SpeI ACTAGT 1 cut(s) 838
SsiI CCGC 1 cut(s) 690
SspI AATATT 2 cut(s) 428, 1570
SspMI CTAG 3 cut(s) 543, 839, 1065
StyD4I CCNGG 3 cut(s) 142, 177, 676
TaaI ACNGT 1 cut(s) 1459
TaiI ACGT 2 cut(s) 623, 1150
TaqI TCGA 6 cut(s) 91, 552, 898, 1311, 1462, 1520
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 2 cut(s) 722, 1010
Tru1I TTAA 4 cut(s) 536, 561, 1022, 1473
Tru9I TTAA 4 cut(s) 536, 561, 1022, 1473
TscAI CASTG 5 cut(s) 265, 497, 617, 1143, 1600
TseFI GTSAC 3 cut(s) 478, 843, 947
TseI GCWGC 9 cut(s) 77, 161, 170, 267, 773, 776, 890, 1175, 1376
Tsp45I GTSAC 3 cut(s) 478, 843, 947
TspDTI ATGAA 3 cut(s) 341, 350, 462
TspGWI ACGGA 1 cut(s) 1016
TspRI CASTG 5 cut(s) 265, 497, 617, 1143, 1600
Vha464I CTTAAG 1 cut(s) 535
VpaK11BI GGWCC 3 cut(s) 120, 212, 1166
XapI RAATTY 4 cut(s) 800, 995, 1209, 1537
XceI RCATGY 2 cut(s) 967, 1130
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 3 cut(s) 543, 839, 1065
Zsp2I ATGCAT 3 cut(s) 12, 523, 655
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.