Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr00

Orthogroup ID

OG0000117

Physical Location & Seq

Genomic Coordinates Reverse (-)

51856105 .. 51858528

Gene Structure 2424 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD00G1219900.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1101 bp

ATGAAGGATATGGAGTTGGATGCTTACCGATTCTCTATTTCATGGTCCAGATTATTACCAAATGGAACGTTAAGTGGTGGCGTTAACAGAAAAGGAATTGATTATTACGACAATCTCATCGATGAACTCCTACGCAATGGATTAAAGCCATTTGTGACACTCTTTCATGGGGATGTTCCCCAAGCTTTAGAAGATGAATATGGTGGTTTCTTAAGCCCTCGTATTGTGAATTTGGTGATCGAGTCAAGCACTGGTTCACGGAAAATGAGCCATATACTTTTAGTTACATGGGTTATGCTGTTGGTACTCAAGTACCGGGACGCTGCTCTTCTTGGCAAAAACCTAAACTGCACCGGTGGAGATTCAGCCATTGAACCATACTTGGTGGCACACCACCTTCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAAGAATAGATATCAGGCAACTCAAAAAGGCTTGATAGGGATAACATTGGTGTCAGACTGGTTTGAGCCTGCTTCGGATTCAAAGCAAGATAAAGATGCTGCCTTACGATCTTTGGATTTTATGTTTGGATGGCAAGTGTTTTTGGACCCATTAACAAGTGGTGACTATCCACACACCATGCGATCAATTGTTGGGAAAAGATTGCCCAAATTCACAAAAAGAACAATCCAACTTAATGGAGCACTCATTGGTCCACAGGCTGCTTCAGATTGGTTATATGTATATCCAAAAGGAATTCACGATCTTGTGCTTTACACAAAGGAAAAATATAATGATCCACTCATTTATATTACTGAGAATGGTGTATCAGAGTTGAATAATCCAGAACTATCACTTGAAGAGGCCCTTCATGATACCAGTAGAATTGACTACTACTATCGTCACCTGTGTTACCTTCAAGCGGCGATCAAAAATGGCGCGAAAGTGAAGGGATACTTTGCATGGACATTGTTAGACGACTTTGAATGGACTGCCGGATACACTGTCCGATTTGGTTTAAACTACGTGGATTATAAAGATGGCCTGAAAAGATACCCAAAACTCTCAGCACATTGGTTCAAAAATTTTCTTAAGAAGAATAAATTCAAAGAAAGCTACAGTTCATTATAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0008150 GO:0008152 GO:0008422 GO:0015926 GO:0016787 GO:0016798 GO:0071704 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

367

Amino Acids

41.79

Weight (kDa)

8.22

Isoelectric Point (pI)

26.23

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	1 - 220	1.9e-48	Glycosyl hydrolase family 1
Glyco_hydro_1	PF00232	228 - 358	4.4e-38	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000117)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca	FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica	MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica	Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis	pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis	RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata	RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora	Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa	Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha	Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana	Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	2 cut(s)	1005, 1099
AccII	CGCG	1 cut(s)	911
AciI	CCGC	1 cut(s)	893
AclI	AACGTT	1 cut(s)	68
AclWI	GGATC	1 cut(s)	761
AcsI	RAATTY	5 cut(s)	229, 641, 726, 1054, 1073
AcuI	CTGAAG	1 cut(s)	681
AfaI	GTAC	3 cut(s)	306, 314, 431
AfiI	CCNNNNNNNGG	1 cut(s)	892
AflII	CTTAAG	2 cut(s)	211, 1061
AgeI	ACCGGT	1 cut(s)	353
AgsI	TTSAA	8 cut(s)	374, 513, 808, 830, 890, 956, 1051, 1078
AluBI	AGCT	3 cut(s)	185, 419, 1086
AluI	AGCT	3 cut(s)	185, 419, 1086
Alw21I	GWGCWC	1 cut(s)	676
AlwI	GGATC	1 cut(s)	761
AoxI	GGCC	2 cut(s)	834, 1012
ApeKI	GCWGC	4 cut(s)	323, 416, 530, 692
ApoI	RAATTY	5 cut(s)	229, 641, 726, 1054, 1073
AsiGI	ACCGGT	1 cut(s)	353
Asp700I	GAANNNNTTC	1 cut(s)	1073
AspLEI	GCGC	1 cut(s)	911
AspS9I	GGNCC	4 cut(s)	45, 577, 683, 835
AsuC2I	CCSGG	1 cut(s)	317
AsuHPI	GGTGA	3 cut(s)	247, 605, 866
AvaII	GGWCC	3 cut(s)	45, 577, 683
BaeI	ACNNNNGTAYC	4 cut(s)	296, 296, 329, 329
Bbv12I	GWGCWC	1 cut(s)	676
BbvI	GCAGC	4 cut(s)	310, 428, 517, 679
BccI	CCATC	2 cut(s)	555, 1004
BciVI	GTATCC	2 cut(s)	917, 962
BcnI	CCSGG	1 cut(s)	317
BfmI	CTRYAG	2 cut(s)	420, 1087
BfrI	CTTAAG	2 cut(s)	211, 1061
BfuI	GTATCC	2 cut(s)	917, 962
BisI	GCNGC	5 cut(s)	324, 417, 531, 693, 894
BlsI	GCNGC	5 cut(s)	325, 418, 532, 694, 895
Bme1390I	CCNGG	1 cut(s)	317
Bme18I	GGWCC	3 cut(s)	45, 577, 683
BmgT120I	GGNCC	4 cut(s)	45, 577, 683, 835
BmiI	GGNNCC	1 cut(s)	579
BmrFI	CCNGG	1 cut(s)	317
BmsI	GCATC	2 cut(s)	10, 517
BpuEI	CTTGAG	1 cut(s)	293
BpuMI	CCSGG	1 cut(s)	317
Bsa29I	ATCGAT	1 cut(s)	120
BsaAI	YACGTR	1 cut(s)	997
BsaWI	WCCGGW	1 cut(s)	353
BsaXI	ACNNNNNCTCC	2 cut(s)	405, 435
Bsc4I	CCNNNNNNNGG	1 cut(s)	892
Bse118I	RCCGGY	1 cut(s)	353
Bse1I	ACTGG	3 cut(s)	256, 494, 849
Bse3DI	GCAATG	1 cut(s)	142
BseCI	ATCGAT	1 cut(s)	120
BseGI	GGATG	3 cut(s)	25, 178, 566
BseLI	CCNNNNNNNGG	1 cut(s)	892
BseMI	GCAATG	1 cut(s)	142
BseMII	CTCAG	2 cut(s)	777, 1050
BseNI	ACTGG	3 cut(s)	256, 494, 849
BseXI	GCAGC	4 cut(s)	310, 428, 517, 679
BsgI	GTGCAG	1 cut(s)	334
Bsh1236I	CGCG	1 cut(s)	911
BshFI	GGCC	2 cut(s)	836, 1014
BshTI	ACCGGT	1 cut(s)	353
BshVI	ATCGAT	1 cut(s)	120
BsiHKAI	GWGCWC	1 cut(s)	676
BsiSI	CCGG	3 cut(s)	316, 354, 966
BslFI	GGGAC	1 cut(s)	332
BslI	CCNNNNNNNGG	1 cut(s)	892
BsmFI	GGGAC	1 cut(s)	332
BsnI	GGCC	2 cut(s)	836, 1014
Bsp1286I	GDGCHC	1 cut(s)	676
Bsp1407I	TGTACA	1 cut(s)	429
Bsp143I	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
BspACI	CCGC	1 cut(s)	893
BspANI	GGCC	2 cut(s)	836, 1014
BspCNI	CTCAG	2 cut(s)	778, 1049
BspDI	ATCGAT	1 cut(s)	120
BspFNI	CGCG	1 cut(s)	911
BspHI	TCATGA	1 cut(s)	841
BspLI	GGNNCC	1 cut(s)	579
BspPI	GGATC	1 cut(s)	761
BspQI	GCTCTTC	1 cut(s)	333
BspTI	CTTAAG	2 cut(s)	211, 1061
BsrDI	GCAATG	1 cut(s)	142
BsrFI	RCCGGY	1 cut(s)	353
BsrGI	TGTACA	1 cut(s)	429
BsrI	ACTGG	3 cut(s)	256, 494, 849
BssAI	RCCGGY	1 cut(s)	353
BssMI	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
Bst4CI	ACNGT	2 cut(s)	976, 1091
Bst6I	CTCTTC	2 cut(s)	333, 825
BstAFI	CTTAAG	2 cut(s)	211, 1061
BstAUI	TGTACA	1 cut(s)	429
BstBAI	YACGTR	1 cut(s)	997
BstC8I	GCNNGC	1 cut(s)	501
BstDEI	CTNAG	2 cut(s)	786, 1036
BstF5I	GGATG	3 cut(s)	25, 178, 566
BstFNI	CGCG	1 cut(s)	911
BstHHI	GCGC	1 cut(s)	911
BstKTI	GATC	6 cut(s)	240, 542, 617, 736, 769, 900
BstMBI	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
BstMWI	GCNNNNNNNGC	1 cut(s)	413
BstSCI	CCNGG	1 cut(s)	315
BstSFI	CTRYAG	2 cut(s)	420, 1087
BstUI	CGCG	1 cut(s)	911
BstV1I	GCAGC	4 cut(s)	310, 428, 517, 679
BstXI	CCANNNNNNTGG	1 cut(s)	668
Bsu15I	ATCGAT	1 cut(s)	120
BsuI	GTATCC	2 cut(s)	917, 962
BsuRI	GGCC	2 cut(s)	836, 1014
BsuTUI	ATCGAT	1 cut(s)	120
BtsCI	GGATG	3 cut(s)	25, 178, 566
BtsIMutI	CAGTG	2 cut(s)	249, 972
Cac8I	GCNNGC	1 cut(s)	501
CciI	TCATGA	1 cut(s)	841
CfoI	GCGC	1 cut(s)	911
Cfr10I	RCCGGY	1 cut(s)	353
Cfr13I	GGNCC	4 cut(s)	45, 577, 683, 835
ClaI	ATCGAT	1 cut(s)	120
CseI	GACGC	1 cut(s)	329
Csp6I	GTAC	3 cut(s)	305, 313, 430
CspAI	ACCGGT	1 cut(s)	353
CviAII	CATG	7 cut(s)	42, 167, 288, 410, 610, 842, 933
CviQI	GTAC	3 cut(s)	305, 313, 430
DdeI	CTNAG	2 cut(s)	786, 1036
DpnI	GATC	6 cut(s)	239, 541, 616, 735, 768, 899
DpnII	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
DraI	TTTAAA	1 cut(s)	990
Eam1104I	CTCTTC	2 cut(s)	333, 825
EarI	CTCTTC	2 cut(s)	333, 825
Eco32I	GATATC	1 cut(s)	443
Eco47I	GGWCC	3 cut(s)	45, 577, 683
Eco57I	CTGAAG	1 cut(s)	681
EcoO109I	RGGNCCY	1 cut(s)	835
EcoRI	GAATTC	1 cut(s)	726
EcoRV	GATATC	1 cut(s)	443
FaeI	CATG	7 cut(s)	45, 170, 291, 413, 613, 845, 936
FalI	AAGNNNNNCTT	4 cut(s)	822, 854, 911, 943
FaqI	GGGAC	1 cut(s)	332
FatI	CATG	7 cut(s)	41, 166, 287, 409, 609, 841, 932
Fnu4HI	GCNGC	5 cut(s)	324, 417, 531, 693, 894
FokI	GGATG	3 cut(s)	32, 185, 573
Fsp4HI	GCNGC	5 cut(s)	324, 417, 531, 693, 894
GlaI	GCGC	1 cut(s)	910
GluI	GCNGC	5 cut(s)	324, 417, 531, 693, 894
HaeIII	GGCC	2 cut(s)	836, 1014
HapII	CCGG	3 cut(s)	316, 354, 966
HgaI	GACGC	1 cut(s)	329
HhaI	GCGC	1 cut(s)	911
Hin1II	CATG	7 cut(s)	45, 170, 291, 413, 613, 845, 936
Hin6I	GCGC	1 cut(s)	909
HinP1I	GCGC	1 cut(s)	909
HincII	GTYRAC	1 cut(s)	85
HindII	GTYRAC	1 cut(s)	85
HindIII	AAGCTT	1 cut(s)	183
HinfI	GANTC	4 cut(s)	30, 242, 362, 509
HpaI	GTTAAC	1 cut(s)	85
HpaII	CCGG	3 cut(s)	316, 354, 966
HphI	GGTGA	3 cut(s)	247, 605, 866
Hpy166II	GTNNAC	3 cut(s)	85, 257, 686
Hpy188I	TCNGA	5 cut(s)	487, 508, 700, 802, 980
Hpy188III	TCNNGA	4 cut(s)	48, 731, 815, 842
Hpy8I	GTNNAC	3 cut(s)	85, 257, 686
HpyAV	CCTTC	4 cut(s)	407, 848, 896, 913
HpyCH4III	ACNGT	2 cut(s)	976, 1091
HpyCH4IV	ACGT	2 cut(s)	68, 996
HpyCH4V	TGCA	2 cut(s)	351, 932
HpyF10VI	GCNNNNNNNGC	1 cut(s)	413
HpyF3I	CTNAG	2 cut(s)	786, 1036
HpySE526I	ACGT	2 cut(s)	68, 996
Hsp92II	CATG	7 cut(s)	45, 170, 291, 413, 613, 845, 936
HspAI	GCGC	1 cut(s)	909
KspAI	GTTAAC	1 cut(s)	85
Kzo9I	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
LguI	GCTCTTC	1 cut(s)	333
LmnI	GCTCC	2 cut(s)	413, 671
Lsp1109I	GCAGC	4 cut(s)	310, 428, 517, 679
LweI	GCATC	2 cut(s)	10, 517
MaeII	ACGT	2 cut(s)	68, 996
MaeIII	GTNAC	5 cut(s)	154, 283, 593, 872, 881
MalI	GATC	6 cut(s)	239, 541, 616, 735, 768, 899
MboI	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
MboII	GAAGA	5 cut(s)	203, 320, 392, 842, 1078
MfeI	CAATTG	1 cut(s)	618
MhlI	GDGCHC	1 cut(s)	676
MluCI	AATT	9 cut(s)	96, 229, 425, 618, 641, 726, 855, 1054, 1073
MlyI	GAGTC	1 cut(s)	251
MmeI	TCCRAC	1 cut(s)	685
MnlI	CCTC	2 cut(s)	228, 826
MroXI	GAANNNNTTC	1 cut(s)	1073
MseI	TTAA	8 cut(s)	71, 84, 143, 212, 584, 666, 989, 1062
MslI	CAYNNNNRTG	1 cut(s)	171
MspA1I	CMGCKG	1 cut(s)	419
MspCI	CTTAAG	2 cut(s)	211, 1061
MspI	CCGG	3 cut(s)	316, 354, 966
MspR9I	CCNGG	1 cut(s)	317
MssI	GTTTAAAC	1 cut(s)	990
MunI	CAATTG	1 cut(s)	618
MvnI	CGCG	1 cut(s)	911
MwoI	GCNNNNNNNGC	1 cut(s)	413
NciI	CCSGG	1 cut(s)	317
NdeII	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
NlaIII	CATG	7 cut(s)	45, 170, 291, 413, 613, 845, 936
NlaIV	GGNNCC	1 cut(s)	579
NmuCI	GTSAC	3 cut(s)	154, 593, 872
PagI	TCATGA	1 cut(s)	841
PciSI	GCTCTTC	1 cut(s)	333
PdmI	GAANNNNTTC	1 cut(s)	1073
PfeI	GAWTC	3 cut(s)	30, 362, 509
PinAI	ACCGGT	1 cut(s)	353
PkrI	GCNGC	5 cut(s)	325, 418, 532, 694, 895
PleI	GAGTC	1 cut(s)	250
PmeI	GTTTAAAC	1 cut(s)	990
PpsI	GAGTC	1 cut(s)	250
Ppu21I	YACGTR	1 cut(s)	997
PsiI	TTATAA	2 cut(s)	1005, 1099
Psp1406I	AACGTT	1 cut(s)	68
PspN4I	GGNNCC	1 cut(s)	579
PspPI	GGNCC	4 cut(s)	45, 577, 683, 835
PvuII	CAGCTG	1 cut(s)	419
RsaI	GTAC	3 cut(s)	306, 314, 431
RsaNI	GTAC	3 cut(s)	305, 313, 430
RseI	CAYNNNNRTG	1 cut(s)	171
SapI	GCTCTTC	1 cut(s)	333
SaqAI	TTAA	8 cut(s)	71, 84, 143, 212, 584, 666, 989, 1062
SatI	GCNGC	5 cut(s)	324, 417, 531, 693, 894
Sau3AI	GATC	6 cut(s)	237, 539, 614, 733, 766, 897
Sau96I	GGNCC	4 cut(s)	45, 577, 683, 835
SchI	GAGTC	1 cut(s)	251
ScrFI	CCNGG	1 cut(s)	317
SduI	GDGCHC	1 cut(s)	676
SetI	ASST	9 cut(s)	71, 187, 345, 399, 421, 879, 888, 999, 1088
SfaNI	GCATC	2 cut(s)	10, 517
SfcI	CTRYAG	2 cut(s)	420, 1087
SgrAI	CRCCGGYG	1 cut(s)	353
SinI	GGWCC	3 cut(s)	45, 577, 683
SmiMI	CAYNNNNRTG	1 cut(s)	171
SmlI	CTYRAG	3 cut(s)	211, 308, 1061
SmoI	CTYRAG	3 cut(s)	211, 308, 1061
Sse9I	AATT	9 cut(s)	96, 229, 425, 618, 641, 726, 855, 1054, 1073
SsiI	CCGC	1 cut(s)	893
StyD4I	CCNGG	1 cut(s)	315
TaaI	ACNGT	2 cut(s)	976, 1091
TaiI	ACGT	2 cut(s)	71, 999
TaqI	TCGA	2 cut(s)	120, 240
TasI	AATT	9 cut(s)	96, 229, 425, 618, 641, 726, 855, 1054, 1073
TatI	WGTACW	1 cut(s)	429
TauI	GCSGC	1 cut(s)	896
TfiI	GAWTC	3 cut(s)	30, 362, 509
Tru1I	TTAA	8 cut(s)	71, 84, 143, 212, 584, 666, 989, 1062
Tru9I	TTAA	8 cut(s)	71, 84, 143, 212, 584, 666, 989, 1062
TscAI	CASTG	2 cut(s)	256, 979
TseFI	GTSAC	3 cut(s)	154, 593, 872
TseI	GCWGC	4 cut(s)	323, 416, 530, 692
Tsp45I	GTSAC	3 cut(s)	154, 593, 872
TspDTI	ATGAA	7 cut(s)	17, 30, 138, 155, 210, 830, 1083
TspGWI	ACGGA	1 cut(s)	274
TspRI	CASTG	2 cut(s)	256, 979
Vha464I	CTTAAG	2 cut(s)	211, 1061
VpaK11BI	GGWCC	3 cut(s)	45, 577, 683
XapI	RAATTY	5 cut(s)	229, 641, 726, 1054, 1073
XmnI	GAANNNNTTC	1 cut(s)	1073

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.