Rroxscaffold_1G00004280

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0000117
Physical Location & Seq
Reverse (-)
5807125 .. 5994417
187293 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00004280.1

Sequence Viewer

Length: 1605 bp
ATGCAAGGAGCTCTGTTTCTAGGCGTAGTGCTTCTACTTGTTGCAGGCTTTAGATTGACAAGCAGTGAAGCTCTTACACCCAGTACTCCTATCTATGACACTGGTTTCCTCAACAGGACTAGTTTCCCAGCAGGTTTCATATTTGGGACAGCTTCAGCGTCATATCAGTATGAAGGTGCTGCTAAAGAAGGTGGTAGAGGACCGAGCATATGGGATACCTACACCCACGAACATCCAGAAAGGATCACGGATGGAAGCAATGGAGATGTAGCTAATGATCAATATCACCACTATAAGGAAGATGTGGGGATTATGAAGAATATGAATTTGGATGCTTATAGGTTCTCTATCTCATGGTCAAGGTTGTTACCAAATGGAAAGCTAAGTGGGGGAGTGAACAAGGAAGGAGTCAAGTACTACAACAATCTCATCAATGAACTCCTAAGCAATGGTCTAAAGCCATTTGTGACCCTCTTTCATTGGGATCTTCCCCAAACTTTAGAAGACGAATATGGCGGTTTCTTAAGCCCTAACGTTGTCATGCATTTTCGGGACTATGCGGAGCTGTGTTACAAGGAATTTGGTGATCGGGTAAAGCACTGGATCACGTTGAATGAGCCATGGACATACAGTAATGGTGGTTATGCAGTCGGGTCATTGGCACCAGGACGGTGTTCTGCGTGGCAGCAGCTAAATTGCACCGGAGGGGATTCGGGTACTGAACCATATTTGGTGGCACACCACTTACTCCTTTCTCATGCAGCTGCTGTAAAGTTGTACAAGCAGAAATATCAGGGCATCTCAAAAGGAGTTATAGGAGTGACCCTAGTGTCTCACTGGTTCATTCCAATTTCAGGGGCAAAGCACAACAAAAATGGAGTGACCCTAGTGTCTCACTGGTTCATTCCAATTTCAGGGGCAAAGCACAACAAAAATGCTGCCTTACGATCTTTGGATTTTATGTTTGGATGGTTCATGGATCCTTTGACAAACGGAGACTATCCACACAGCATGCGATCTCTCGTCGGAAACCGATTACCCAAATTCACAAAAGAACAATCCAAGTTACTAATCGGATCATGTGATTTTCTTGGACTAAATTACTATACTACTTACTATGCAACATATACACCTCAGAACAATTCTTTAAATGCAAGCTACTTGACAGACGCTCGTGCTACTCAATCATTTGCGCTAAATGGCGTCCCCATTGGTCCACAGGCTGCTTCATCATGGCTATATGTTTATCCGAAAGGAATTAGAGACCTTTTACTCTACAAAAAGACAAAGTATAATGATCCACTTATTTACATTACTGAGAATGGCATTGATGAGTTCAATGATCCCAAATTATCACTTGAGGAAGCCCTCTATGACACTCACAGAGTTGACTATTACTTCCGCCACCTCTATTACCTTCAAAAAGCAATCAAGGAAGGTGTGAACTTGAAGGGCTACTTTGCATGGTCATTGCTAGACAACTACGAATGGGGTTCAGGTTACACAGTTCGATTTGGTATCAACTATGTGGATTACAAAAATGGAAACAAAAGGCACCCAAAACTCTCGGCACATTGGTTCAAAAACTTCCTCAAGAAGTATTGA

Protein Analysis

534

Amino Acids

60.49

Weight (kDa)

8.72

Isoelectric Point (pI)

23.18

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 40 - 289 3.4e-92 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 291 - 532 2.2e-59 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 829, 889
Acc36I ACCTGC 1 cut(s) 122
AccB1I GGYRCC 2 cut(s) 661, 1554
AciI CCGC 3 cut(s) 516, 560, 1402
AclI AACGTT 1 cut(s) 534
AclWI GGATC 8 cut(s) 251, 492, 611, 974, 987, 1084, 1292, 1337
AcsI RAATTY 3 cut(s) 325, 578, 1043
AcuI CTGAAG 1 cut(s) 138
AcyI GRCGYC 1 cut(s) 1203
AfaI GTAC 4 cut(s) 85, 416, 718, 779
AfiI CCNNNNNNNGG 3 cut(s) 295, 854, 914
AflII CTTAAG 1 cut(s) 523
AgsI TTSAA 5 cut(s) 613, 1339, 1421, 1450, 1582
AhlI ACTAGT 1 cut(s) 119
AjnI CCWGG 1 cut(s) 664
AjuI GAANNNNNNNTTGG 2 cut(s) 311, 343
AluBI AGCT 9 cut(s) 11, 71, 152, 272, 382, 565, 691, 764, 1158
AluI AGCT 9 cut(s) 11, 71, 152, 272, 382, 565, 691, 764, 1158
Alw21I GWGCWC 1 cut(s) 13
Alw26I GTCTC 4 cut(s) 837, 897, 990, 1257
AlwI GGATC 8 cut(s) 251, 492, 611, 974, 987, 1084, 1292, 1337
AlwNI CAGNNNCTG 1 cut(s) 767
ApeKI GCWGC 7 cut(s) 179, 685, 688, 761, 764, 938, 1223
ApoI RAATTY 3 cut(s) 325, 578, 1043
AspLEI GCGC 1 cut(s) 1195
AspS9I GGNCC 2 cut(s) 200, 1214
AsuHPI GGTGA 2 cut(s) 278, 596
AvaII GGWCC 2 cut(s) 200, 1214
BamHI GGATCC 1 cut(s) 979
BanI GGYRCC 2 cut(s) 661, 1554
BanII GRGCYC 1 cut(s) 13
BauI CACGAG 1 cut(s) 1173
BbsI GAAGAC 1 cut(s) 510
Bbv12I GWGCWC 1 cut(s) 13
BbvI GCAGC 7 cut(s) 166, 697, 700, 751, 773, 925, 1210
BccI CCATC 2 cut(s) 245, 963
BciT130I CCWGG 1 cut(s) 666
BciVI GTATCC 1 cut(s) 208
BclI TGATCA 1 cut(s) 277
BcoDI GTCTC 4 cut(s) 837, 897, 990, 1257
BcuI ACTAGT 1 cut(s) 119
BfaI CTAG 5 cut(s) 20, 120, 827, 887, 1475
BfrI CTTAAG 1 cut(s) 523
BfuAI ACCTGC 1 cut(s) 122
BfuI GTATCC 1 cut(s) 208
BisI GCNGC 7 cut(s) 180, 686, 689, 762, 765, 939, 1224
BlsI GCNGC 7 cut(s) 181, 687, 690, 763, 766, 940, 1225
BmcAI AGTACT 2 cut(s) 85, 416
Bme1390I CCNGG 1 cut(s) 666
Bme18I GGWCC 2 cut(s) 200, 1214
BmgT120I GGNCC 2 cut(s) 200, 1214
BmiI GGNNCC 3 cut(s) 663, 981, 1556
BmrFI CCNGG 1 cut(s) 666
BmrI ACTGGG 1 cut(s) 75
BmsI GCATC 2 cut(s) 322, 807
BmuI ACTGGG 1 cut(s) 75
BpiI GAAGAC 1 cut(s) 510
Bpu10I CCTNAGC 1 cut(s) 443
BpuEI CTTGAG 2 cut(s) 1379, 1577
BsaBI GATNNNNATC 1 cut(s) 282
BsaHI GRCGYC 1 cut(s) 1203
BsaI GGTCTC 1 cut(s) 1257
BsaJI CCNNGG 1 cut(s) 620
BsaWI WCCGGW 1 cut(s) 701
Bsc4I CCNNNNNNNGG 3 cut(s) 295, 854, 914
Bse1I ACTGG 5 cut(s) 81, 106, 605, 842, 902
Bse3DI GCAATG 3 cut(s) 265, 454, 1469
Bse8I GATNNNNATC 1 cut(s) 282
BseBI CCWGG 1 cut(s) 666
BseDI CCNNGG 1 cut(s) 620
BseGI GGATG 4 cut(s) 232, 256, 337, 974
BseJI GATNNNNATC 1 cut(s) 282
BseLI CCNNNNNNNGG 3 cut(s) 295, 854, 914
BseMI GCAATG 3 cut(s) 265, 454, 1469
BseMII CTCAG 2 cut(s) 1148, 1308
BseNI ACTGG 5 cut(s) 81, 106, 605, 842, 902
BseXI GCAGC 7 cut(s) 166, 697, 700, 751, 773, 925, 1210
BseYI CCCAGC 1 cut(s) 127
BshNI GGYRCC 2 cut(s) 661, 1554
BsiHKAI GWGCWC 1 cut(s) 13
BsiSI CCGG 1 cut(s) 702
BslFI GGGAC 3 cut(s) 160, 566, 1190
BslI CCNNNNNNNGG 3 cut(s) 295, 854, 914
BsmAI GTCTC 4 cut(s) 837, 897, 990, 1257
BsmFI GGGAC 3 cut(s) 160, 566, 1190
Bso31I GGTCTC 1 cut(s) 1257
Bsp1286I GDGCHC 1 cut(s) 13
Bsp1407I TGTACA 1 cut(s) 777
Bsp19I CCATGG 1 cut(s) 620
BspACI CCGC 3 cut(s) 516, 560, 1402
BspCNI CTCAG 2 cut(s) 1147, 1309
BspLI GGNNCC 3 cut(s) 663, 981, 1556
BspMI ACCTGC 1 cut(s) 122
BspPI GGATC 8 cut(s) 251, 492, 611, 974, 987, 1084, 1292, 1337
BspT107I GGYRCC 2 cut(s) 661, 1554
BspTI CTTAAG 1 cut(s) 523
BspTNI GGTCTC 1 cut(s) 1257
BsrDI GCAATG 3 cut(s) 265, 454, 1469
BsrGI TGTACA 1 cut(s) 777
BsrI ACTGG 5 cut(s) 81, 106, 605, 842, 902
BssECI CCNNGG 1 cut(s) 620
BssNI GRCGYC 1 cut(s) 1203
BssSI CACGAG 1 cut(s) 1173
BssT1I CCWWGG 1 cut(s) 620
Bst2BI CACGAG 1 cut(s) 1173
Bst2UI CCWGG 1 cut(s) 666
Bst4CI ACNGT 3 cut(s) 632, 672, 1507
BstACI GRCGYC 1 cut(s) 1203
BstAFI CTTAAG 1 cut(s) 523
BstAUI TGTACA 1 cut(s) 777
BstC8I GCNNGC 3 cut(s) 46, 1013, 1156
BstDEI CTNAG 4 cut(s) 383, 443, 1134, 1317
BstDSI CCRYGG 1 cut(s) 620
BstF5I GGATG 4 cut(s) 232, 256, 337, 974
BstHHI GCGC 1 cut(s) 1195
BstMAI GTCTC 4 cut(s) 837, 897, 990, 1257
BstNI CCWGG 1 cut(s) 666
BstNSI RCATGY 1 cut(s) 1015
BstSCI CCNGG 1 cut(s) 664
BstV1I GCAGC 7 cut(s) 166, 697, 700, 751, 773, 925, 1210
BstV2I GAAGAC 1 cut(s) 510
BstX2I RGATCY 2 cut(s) 484, 979
BstYI RGATCY 2 cut(s) 484, 979
BsuI GTATCC 1 cut(s) 208
BtgI CCRYGG 1 cut(s) 620
BtsCI GGATG 4 cut(s) 232, 256, 337, 974
BtsI GCAGTG 1 cut(s) 70
BtsIMutI CAGTG 5 cut(s) 70, 99, 598, 835, 895
BveI ACCTGC 1 cut(s) 122
Cac8I GCNNGC 3 cut(s) 46, 1013, 1156
CaiI CAGNNNCTG 1 cut(s) 767
CfoI GCGC 1 cut(s) 1195
Cfr13I GGNCC 2 cut(s) 200, 1214
CseI GACGC 3 cut(s) 147, 1178, 1192
Csp6I GTAC 4 cut(s) 84, 415, 717, 778
CviAII CATG 9 cut(s) 354, 541, 621, 758, 976, 1012, 1080, 1233, 1464
CviQI GTAC 4 cut(s) 84, 415, 717, 778
DdeI CTNAG 4 cut(s) 383, 443, 1134, 1317
DraI TTTAAA 1 cut(s) 1149
DrdI GACNNNNNNGTC 2 cut(s) 829, 889
DseDI GACNNNNNNGTC 2 cut(s) 829, 889
EciI GGCGGA 1 cut(s) 1391
Ecl136II GAGCTC 1 cut(s) 11
Eco130I CCWWGG 1 cut(s) 620
Eco24I GRGCYC 1 cut(s) 13
Eco31I GGTCTC 1 cut(s) 1257
Eco47I GGWCC 2 cut(s) 200, 1214
Eco53kI GAGCTC 1 cut(s) 11
Eco57I CTGAAG 1 cut(s) 138
EcoICRI GAGCTC 1 cut(s) 11
EcoRII CCWGG 1 cut(s) 664
EcoT14I CCWWGG 1 cut(s) 620
EcoT22I ATGCAT 1 cut(s) 546
EcoT38I GRGCYC 1 cut(s) 13
ErhI CCWWGG 1 cut(s) 620
FaeI CATG 9 cut(s) 357, 544, 624, 761, 979, 1015, 1083, 1236, 1467
FalI AAGNNNNNCTT 2 cut(s) 1442, 1474
FaqI GGGAC 3 cut(s) 160, 566, 1190
FatI CATG 9 cut(s) 353, 540, 620, 757, 975, 1011, 1079, 1232, 1463
FauNDI CATATG 1 cut(s) 209
FbaI TGATCA 1 cut(s) 277
Fnu4HI GCNGC 7 cut(s) 180, 686, 689, 762, 765, 939, 1224
FokI GGATG 4 cut(s) 219, 263, 344, 981
FriOI GRGCYC 1 cut(s) 13
Fsp4HI GCNGC 7 cut(s) 180, 686, 689, 762, 765, 939, 1224
FspBI CTAG 5 cut(s) 20, 120, 827, 887, 1475
GlaI GCGC 1 cut(s) 1194
GluI GCNGC 7 cut(s) 180, 686, 689, 762, 765, 939, 1224
GsaI CCCAGC 1 cut(s) 131
HapII CCGG 1 cut(s) 702
HgaI GACGC 3 cut(s) 147, 1178, 1192
HhaI GCGC 1 cut(s) 1195
Hin1I GRCGYC 1 cut(s) 1203
Hin1II CATG 9 cut(s) 357, 544, 624, 761, 979, 1015, 1083, 1236, 1467
Hin6I GCGC 1 cut(s) 1193
HinP1I GCGC 1 cut(s) 1193
HincII GTYRAC 1 cut(s) 1390
HindII GTYRAC 1 cut(s) 1390
HinfI GANTC 2 cut(s) 408, 710
HpaII CCGG 1 cut(s) 702
HphI GGTGA 2 cut(s) 278, 596
Hpy166II GTNNAC 4 cut(s) 397, 1217, 1390, 1444
Hpy188I TCNGA 4 cut(s) 1028, 1076, 1137, 1251
Hpy188III TCNNGA 3 cut(s) 236, 551, 1594
Hpy8I GTNNAC 4 cut(s) 397, 1217, 1390, 1444
Hpy99I CGWCG 1 cut(s) 1028
HpyAV CCTTC 6 cut(s) 167, 182, 398, 1427, 1430, 1444
HpyCH4III ACNGT 3 cut(s) 632, 672, 1507
HpyCH4IV ACGT 2 cut(s) 534, 608
HpyCH4V TGCA 9 cut(s) 4, 44, 544, 647, 699, 761, 1121, 1154, 1463
HpyF3I CTNAG 4 cut(s) 383, 443, 1134, 1317
HpySE526I ACGT 2 cut(s) 534, 608
Hsp92I GRCGYC 1 cut(s) 1203
Hsp92II CATG 9 cut(s) 357, 544, 624, 761, 979, 1015, 1083, 1236, 1467
HspAI GCGC 1 cut(s) 1193
Ksp22I TGATCA 1 cut(s) 277
LmnI GCTCC 2 cut(s) 8, 562
Lsp1109I GCAGC 7 cut(s) 166, 697, 700, 751, 773, 925, 1210
LweI GCATC 2 cut(s) 322, 807
MaeI CTAG 5 cut(s) 20, 120, 827, 887, 1475
MaeII ACGT 2 cut(s) 534, 608
MaeIII GTNAC 7 cut(s) 366, 466, 569, 820, 880, 1065, 1499
MboII GAAGA 4 cut(s) 311, 328, 479, 515
MflI RGATCY 2 cut(s) 484, 979
MhlI GDGCHC 1 cut(s) 13
MlyI GAGTC 1 cut(s) 417
MmeI TCCRAC 1 cut(s) 1006
MnlI CCTC 9 cut(s) 119, 191, 482, 698, 1143, 1354, 1379, 1418, 1601
Mph1103I ATGCAT 1 cut(s) 546
MseI TTAA 2 cut(s) 524, 1148
MspA1I CMGCKG 1 cut(s) 764
MspCI CTTAAG 1 cut(s) 523
MspI CCGG 1 cut(s) 702
MspR9I CCNGG 1 cut(s) 666
MvaI CCWGG 1 cut(s) 666
NcoI CCATGG 1 cut(s) 620
NdeI CATATG 1 cut(s) 209
NlaIII CATG 9 cut(s) 357, 544, 624, 761, 979, 1015, 1083, 1236, 1467
NlaIV GGNNCC 3 cut(s) 663, 981, 1556
NmeAIII GCCGAG 1 cut(s) 1547
NmuCI GTSAC 3 cut(s) 466, 820, 880
NsiI ATGCAT 1 cut(s) 546
NspI RCATGY 1 cut(s) 1015
PaeI GCATGC 1 cut(s) 1015
PfeI GAWTC 1 cut(s) 710
PkrI GCNGC 7 cut(s) 181, 687, 690, 763, 766, 940, 1225
PleI GAGTC 1 cut(s) 416
PpsI GAGTC 1 cut(s) 416
Psp124BI GAGCTC 1 cut(s) 13
Psp1406I AACGTT 1 cut(s) 534
Psp6I CCWGG 1 cut(s) 664
PspFI CCCAGC 1 cut(s) 127
PspGI CCWGG 1 cut(s) 664
PspN4I GGNNCC 3 cut(s) 663, 981, 1556
PspPI GGNCC 2 cut(s) 200, 1214
PstNI CAGNNNCTG 1 cut(s) 767
PsuI RGATCY 2 cut(s) 484, 979
PvuII CAGCTG 1 cut(s) 764
RsaI GTAC 4 cut(s) 85, 416, 718, 779
RsaNI GTAC 4 cut(s) 84, 415, 717, 778
SacI GAGCTC 1 cut(s) 13
SaqAI TTAA 2 cut(s) 524, 1148
SatI GCNGC 7 cut(s) 180, 686, 689, 762, 765, 939, 1224
Sau96I GGNCC 2 cut(s) 200, 1214
ScaI AGTACT 2 cut(s) 85, 416
SchI GAGTC 1 cut(s) 417
ScrFI CCNGG 1 cut(s) 666
SduI GDGCHC 1 cut(s) 13
SfaNI GCATC 2 cut(s) 322, 807
SinI GGWCC 2 cut(s) 200, 1214
SmlI CTYRAG 3 cut(s) 523, 1358, 1592
SmoI CTYRAG 3 cut(s) 523, 1358, 1592
SpeI ACTAGT 1 cut(s) 119
SphI GCATGC 1 cut(s) 1015
SsiI CCGC 3 cut(s) 516, 560, 1402
SspMI CTAG 5 cut(s) 20, 120, 827, 887, 1475
SstI GAGCTC 1 cut(s) 13
StyD4I CCNGG 1 cut(s) 664
StyI CCWWGG 1 cut(s) 620
TaaI ACNGT 3 cut(s) 632, 672, 1507
TaiI ACGT 2 cut(s) 537, 611
TaqI TCGA 1 cut(s) 1510
TaqII GACCGA 1 cut(s) 217
TatI WGTACW 3 cut(s) 83, 414, 777
TfiI GAWTC 1 cut(s) 710
Tru1I TTAA 2 cut(s) 524, 1148
Tru9I TTAA 2 cut(s) 524, 1148
TscAI CASTG 5 cut(s) 70, 106, 605, 842, 902
TseFI GTSAC 3 cut(s) 466, 820, 880
TseI GCWGC 7 cut(s) 179, 685, 688, 761, 764, 938, 1223
Tsp45I GTSAC 3 cut(s) 466, 820, 880
TspGWI ACGGA 2 cut(s) 263, 1008
TspRI CASTG 5 cut(s) 70, 106, 605, 842, 902
Vha464I CTTAAG 1 cut(s) 523
VpaK11BI GGWCC 2 cut(s) 200, 1214
XapI RAATTY 3 cut(s) 325, 578, 1043
XceI RCATGY 1 cut(s) 1015
XspI CTAG 5 cut(s) 20, 120, 827, 887, 1475
ZrmI AGTACT 2 cut(s) 85, 416
Zsp2I ATGCAT 1 cut(s) 546
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.