MD15G1374300.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr15
OG0000117
Physical Location & Seq
Forward (+)
45751562 .. 45754870
3309 bp
Loading structure...
UTR
Exon/CDS
Intron
MD15G1374300.v1.1.491

Sequence Viewer

Length: 1581 bp
ATGGCAGTGAAATATTCAAGATCTTTGCTCTTTGGTGTGCTACTACTCATTGGCTTTGCATTGACAAATAGCAAAGCTGCTAATATGGATCCACCTGTGCATTGCGAATTTCTCAATCGCAACAGTTTTGAACCAGGGTTCATATTTGGCACAGGTTCTGCATCTTACCAGTATGAAGGTGCAGTAAAAGAAGATGGAAGAGGACCAAGCATATGGGACACCTACACCCACAAACATCCAGAAAAAATCGCTGATGGCAGTAACGGAGACGTTGCTATTGATCAATATCACCGCTATAAGGAAGATGTGGGGATTATGAAGGATATGGAGTTGGATGCTTACCGATTCTCTATTTCATGGTCCAGATTATTACCAAATGGAACGTTAAGTGGTGGCGTTAACAGAAAAGGAATTGATTATTACAACAATCTCATCGATGAACTCCTAGGCAATGGATTAAAGCCATTTGTGACACTCTTTCATGGGGATGTTCCCCAAGCTTTAGAAGATGAATATGGTGGTTTCTTAAGCCCTCGTATTGTCGATCATTTTAAAGACTATGCGGAACTTTGTTATAAGGAATTTGGTGATCGAGTCCAGCACTGGTTCACAGAAAATGAGCCATATACTTTTAGCTACATGGGTTATGCTGTTGGTACTGAAGTACCGGGACGCTGCTCTTTTTGGCAAAACCTAAACTGCACCGGTGGAGATTCAGCCATTGAGCCATACTTGGTGGCACACCACCTTCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAAGAACAGATATCAGGCAACTCAAAAAGGCTTGATAGGGATAACATTGGTGTCAGACTGGTTTGAGCCTGCTTCGGATTCAAAGCAAGACAAAGATGCTGCCTTACGATCTTTGGATTTTATGTTTGGATGGCAAGTGTTTTTGGACCCATTAACAAGTGGTGACTATCCACACACCATGCGATCAATTGTTGGGAAAAGATTGCCCAAATTCACAAAAGAACAATCCAAGTTGCTAAACGGATCATTTGATTTTCTTGGAATAAATTATTATACTGCTAGATACACAAGTAGTGCACCCAAGAACAATTCACTACCGGCAAGCTACGTAACAGATTCTCGAGCTGATCTTACAACTGAGCTTAATGGAGTACTCATTGGTCCACAGGCTGCTTCAGATTGGTTATATGTATATCCAAAAGGAATTCACGATCTTGTGCTTTACACAAAGGAAAAATATAATGATCCACTCATTTATATTACTGAGAATGGTGTATCAGAGTTGAATAATCCAGAACTATCACTTGAAGAGGCCCTTCATGATACCGGTAGAATTGACTACTACTATCGTCACCTGTGTTACCTTCAAGCGGCGATCAAAAATGGGGCGAAAGTGAAGGGATACTTTGCATGGGCATTGTTAGACAACTTTGAATGGACTTCTGGATACACTGTCCGATTTGGTTTAAACTACGTGGATTATAAAGATGGGCTGAAAAGATACCCAAAACTCTCAGCACACTGGTTCAAAAATTTGCTTAAGAAGAATAAATTCAAAGAAAGCTACAGTTCATTATAA

Protein Analysis

527

Amino Acids

59.72

Weight (kDa)

5.95

Isoelectric Point (pI)

22.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 40 - 518 5.3e-153 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 576, 1485, 1579
AciI CCGC 3 cut(s) 292, 563, 1373
AclI AACGTT 1 cut(s) 383
AclWI GGATC 4 cut(s) 83, 96, 1033, 1241
AcsI RAATTY 6 cut(s) 107, 581, 992, 1206, 1534, 1553
AcuI CTGAAG 2 cut(s) 681, 1161
AfaI GTAC 4 cut(s) 658, 666, 782, 1155
AfiI CCNNNNNNNGG 2 cut(s) 298, 1372
AflII CTTAAG 2 cut(s) 526, 1541
AgeI ACCGGT 2 cut(s) 704, 1328
AgsI TTSAA 9 cut(s) 18, 131, 864, 1288, 1310, 1370, 1436, 1531, 1558
AjnI CCWGG 1 cut(s) 133
AluBI AGCT 8 cut(s) 77, 500, 636, 770, 1107, 1127, 1144, 1566
AluI AGCT 8 cut(s) 77, 500, 636, 770, 1107, 1127, 1144, 1566
Alw21I GWGCWC 1 cut(s) 1081
Alw26I GTCTC 1 cut(s) 261
Alw44I GTGCAC 1 cut(s) 1077
AlwI GGATC 4 cut(s) 83, 96, 1033, 1241
AlwNI CAGNNNCTG 1 cut(s) 158
Ama87I CYCGRG 1 cut(s) 1122
AoxI GGCC 1 cut(s) 1314
ApaLI GTGCAC 1 cut(s) 1077
ApeKI GCWGC 5 cut(s) 77, 675, 767, 881, 1172
ApoI RAATTY 6 cut(s) 107, 581, 992, 1206, 1534, 1553
AsiGI ACCGGT 2 cut(s) 704, 1328
Asp700I GAANNNNTTC 1 cut(s) 1553
AspA2I CCTAGG 1 cut(s) 445
AspS9I GGNCC 5 cut(s) 203, 360, 928, 1163, 1315
AsuC2I CCSGG 1 cut(s) 669
AsuHPI GGTGA 4 cut(s) 281, 599, 956, 1346
AvaI CYCGRG 1 cut(s) 1122
AvaII GGWCC 4 cut(s) 203, 360, 928, 1163
AvrII CCTAGG 1 cut(s) 445
BaeGI GKGCMC 1 cut(s) 1081
BaeI ACNNNNGTAYC 6 cut(s) 648, 648, 681, 681, 1057, 1090
BamHI GGATCC 1 cut(s) 88
Bbv12I GWGCWC 1 cut(s) 1081
BbvI GCAGC 5 cut(s) 64, 662, 779, 868, 1159
BccI CCATC 4 cut(s) 188, 248, 906, 1484
BciT130I CCWGG 1 cut(s) 135
BciVI GTATCC 2 cut(s) 1397, 1442
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 1 cut(s) 669
BcoDI GTCTC 1 cut(s) 261
BfaI CTAG 2 cut(s) 446, 1062
BfmI CTRYAG 2 cut(s) 771, 1567
BfrI CTTAAG 2 cut(s) 526, 1541
BfuI GTATCC 2 cut(s) 1397, 1442
BglII AGATCT 1 cut(s) 20
BisI GCNGC 6 cut(s) 78, 676, 768, 882, 1173, 1374
BlnI CCTAGG 1 cut(s) 445
BlsI GCNGC 6 cut(s) 79, 677, 769, 883, 1174, 1375
BmcAI AGTACT 1 cut(s) 1155
Bme1390I CCNGG 2 cut(s) 135, 669
Bme18I GGWCC 4 cut(s) 203, 360, 928, 1163
BmeT110I CYCGRG 1 cut(s) 1122
BmgT120I GGNCC 5 cut(s) 203, 360, 928, 1163, 1315
BmiI GGNNCC 2 cut(s) 90, 930
BmrFI CCNGG 2 cut(s) 135, 669
BmsI GCATC 3 cut(s) 170, 325, 868
BpuMI CCSGG 1 cut(s) 669
Bsa29I ATCGAT 1 cut(s) 435
BsaAI YACGTR 2 cut(s) 1111, 1477
BsaBI GATNNNNATC 1 cut(s) 285
BsaJI CCNNGG 2 cut(s) 134, 445
BsaWI WCCGGW 2 cut(s) 704, 1328
BsaXI ACNNNNNCTCC 2 cut(s) 756, 786
Bsc4I CCNNNNNNNGG 2 cut(s) 298, 1372
Bse118I RCCGGY 3 cut(s) 704, 1099, 1328
Bse1I ACTGG 4 cut(s) 169, 608, 845, 1529
Bse3DI GCAATG 2 cut(s) 100, 457
Bse8I GATNNNNATC 1 cut(s) 285
BseBI CCWGG 1 cut(s) 135
BseCI ATCGAT 1 cut(s) 435
BseDI CCNNGG 2 cut(s) 134, 445
BseGI GGATG 4 cut(s) 235, 340, 493, 917
BseJI GATNNNNATC 1 cut(s) 285
BseLI CCNNNNNNNGG 2 cut(s) 298, 1372
BseMI GCAATG 2 cut(s) 100, 457
BseMII CTCAG 3 cut(s) 1131, 1257, 1530
BseNI ACTGG 4 cut(s) 169, 608, 845, 1529
BseSI GKGCMC 1 cut(s) 1081
BseXI GCAGC 5 cut(s) 64, 662, 779, 868, 1159
BsgI GTGCAG 2 cut(s) 201, 685
BshFI GGCC 1 cut(s) 1316
BshTI ACCGGT 2 cut(s) 704, 1328
BshVI ATCGAT 1 cut(s) 435
BsiHKAI GWGCWC 1 cut(s) 1081
BsiHKCI CYCGRG 1 cut(s) 1122
BsiSI CCGG 4 cut(s) 668, 705, 1100, 1329
BslFI GGGAC 2 cut(s) 230, 684
BslI CCNNNNNNNGG 2 cut(s) 298, 1372
BsmAI GTCTC 1 cut(s) 261
BsmBI CGTCTC 1 cut(s) 261
BsmFI GGGAC 2 cut(s) 230, 684
BsnI GGCC 1 cut(s) 1316
BsoBI CYCGRG 1 cut(s) 1122
Bsp1286I GDGCHC 1 cut(s) 1081
Bsp1407I TGTACA 1 cut(s) 780
BspACI CCGC 3 cut(s) 292, 563, 1373
BspANI GGCC 1 cut(s) 1316
BspCNI CTCAG 3 cut(s) 1132, 1258, 1529
BspDI ATCGAT 1 cut(s) 435
BspHI TCATGA 1 cut(s) 1321
BspLI GGNNCC 2 cut(s) 90, 930
BspPI GGATC 4 cut(s) 83, 96, 1033, 1241
BspTI CTTAAG 2 cut(s) 526, 1541
BsrDI GCAATG 2 cut(s) 100, 457
BsrFI RCCGGY 3 cut(s) 704, 1099, 1328
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 4 cut(s) 169, 608, 845, 1529
BssAI RCCGGY 3 cut(s) 704, 1099, 1328
BssECI CCNNGG 2 cut(s) 134, 445
BssT1I CCWWGG 1 cut(s) 445
Bst2UI CCWGG 1 cut(s) 135
Bst4CI ACNGT 3 cut(s) 125, 1456, 1571
Bst6I CTCTTC 2 cut(s) 193, 1305
BstAFI CTTAAG 2 cut(s) 526, 1541
BstAUI TGTACA 1 cut(s) 780
BstBAI YACGTR 2 cut(s) 1111, 1477
BstC8I GCNNGC 2 cut(s) 852, 1105
BstDEI CTNAG 3 cut(s) 1140, 1266, 1516
BstF5I GGATG 4 cut(s) 235, 340, 493, 917
BstMAI GTCTC 1 cut(s) 261
BstMWI GCNNNNNNNGC 1 cut(s) 764
BstNI CCWGG 1 cut(s) 135
BstSCI CCNGG 2 cut(s) 133, 667
BstSFI CTRYAG 2 cut(s) 771, 1567
BstSLI GKGCMC 1 cut(s) 1081
BstSNI TACGTA 1 cut(s) 1111
BstV1I GCAGC 5 cut(s) 64, 662, 779, 868, 1159
BstX2I RGATCY 2 cut(s) 20, 88
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 2 cut(s) 20, 88
Bsu15I ATCGAT 1 cut(s) 435
BsuI GTATCC 2 cut(s) 1397, 1442
BsuRI GGCC 1 cut(s) 1316
BsuTUI ATCGAT 1 cut(s) 435
BtsCI GGATG 4 cut(s) 235, 340, 493, 917
BtsI GCAGTG 1 cut(s) 12
BtsIMutI CAGTG 4 cut(s) 12, 601, 1452, 1522
Cac8I GCNNGC 2 cut(s) 852, 1105
CaiI CAGNNNCTG 1 cut(s) 158
CciI TCATGA 1 cut(s) 1321
Cfr10I RCCGGY 3 cut(s) 704, 1099, 1328
Cfr13I GGNCC 5 cut(s) 203, 360, 928, 1163, 1315
ClaI ATCGAT 1 cut(s) 435
CseI GACGC 1 cut(s) 681
Csp6I GTAC 4 cut(s) 657, 665, 781, 1154
CspAI ACCGGT 2 cut(s) 704, 1328
CviAII CATG 7 cut(s) 357, 482, 640, 761, 961, 1322, 1413
CviQI GTAC 4 cut(s) 657, 665, 781, 1154
DdeI CTNAG 3 cut(s) 1140, 1266, 1516
DraI TTTAAA 2 cut(s) 553, 1470
Eam1104I CTCTTC 2 cut(s) 193, 1305
EarI CTCTTC 2 cut(s) 193, 1305
Eco105I TACGTA 1 cut(s) 1111
Eco130I CCWWGG 1 cut(s) 445
Eco32I GATATC 1 cut(s) 794
Eco47I GGWCC 4 cut(s) 203, 360, 928, 1163
Eco57I CTGAAG 2 cut(s) 681, 1161
Eco88I CYCGRG 1 cut(s) 1122
EcoO109I RGGNCCY 1 cut(s) 1315
EcoRI GAATTC 1 cut(s) 1206
EcoRII CCWGG 1 cut(s) 133
EcoRV GATATC 1 cut(s) 794
EcoT14I CCWWGG 1 cut(s) 445
ErhI CCWWGG 1 cut(s) 445
Esp3I CGTCTC 1 cut(s) 261
FaeI CATG 7 cut(s) 360, 485, 643, 764, 964, 1325, 1416
FalI AAGNNNNNCTT 4 cut(s) 1302, 1334, 1391, 1423
FaqI GGGAC 2 cut(s) 230, 684
FatI CATG 7 cut(s) 356, 481, 639, 760, 960, 1321, 1412
FauNDI CATATG 1 cut(s) 212
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 6 cut(s) 78, 676, 768, 882, 1173, 1374
FokI GGATG 4 cut(s) 222, 347, 500, 924
Fsp4HI GCNGC 6 cut(s) 78, 676, 768, 882, 1173, 1374
FspBI CTAG 2 cut(s) 446, 1062
GluI GCNGC 6 cut(s) 78, 676, 768, 882, 1173, 1374
HaeIII GGCC 1 cut(s) 1316
HapII CCGG 4 cut(s) 668, 705, 1100, 1329
HgaI GACGC 1 cut(s) 681
Hin1II CATG 7 cut(s) 360, 485, 643, 764, 964, 1325, 1416
HincII GTYRAC 1 cut(s) 400
HindII GTYRAC 1 cut(s) 400
HindIII AAGCTT 1 cut(s) 498
HinfI GANTC 5 cut(s) 345, 594, 713, 860, 1118
HpaI GTTAAC 1 cut(s) 400
HpaII CCGG 4 cut(s) 668, 705, 1100, 1329
HphI GGTGA 4 cut(s) 281, 599, 956, 1346
Hpy166II GTNNAC 4 cut(s) 400, 609, 1079, 1166
Hpy188I TCNGA 5 cut(s) 838, 859, 1180, 1282, 1460
Hpy188III TCNNGA 8 cut(s) 18, 239, 363, 1122, 1211, 1295, 1322, 1446
Hpy8I GTNNAC 4 cut(s) 400, 609, 1079, 1166
HpyAV CCTTC 6 cut(s) 170, 313, 758, 1328, 1376, 1393
HpyCH4III ACNGT 3 cut(s) 125, 1456, 1571
HpyCH4IV ACGT 4 cut(s) 270, 383, 1110, 1476
HpyCH4V TGCA 7 cut(s) 59, 100, 161, 182, 702, 1079, 1412
HpyF10VI GCNNNNNNNGC 1 cut(s) 764
HpyF3I CTNAG 3 cut(s) 1140, 1266, 1516
HpySE526I ACGT 4 cut(s) 270, 383, 1110, 1476
Hsp92II CATG 7 cut(s) 360, 485, 643, 764, 964, 1325, 1416
Ksp22I TGATCA 1 cut(s) 280
KspAI GTTAAC 1 cut(s) 400
LmnI GCTCC 1 cut(s) 764
Lsp1109I GCAGC 5 cut(s) 64, 662, 779, 868, 1159
LweI GCATC 3 cut(s) 170, 325, 868
MaeI CTAG 2 cut(s) 446, 1062
MaeII ACGT 4 cut(s) 270, 383, 1110, 1476
MaeIII GTNAC 6 cut(s) 260, 469, 944, 1111, 1352, 1361
MboII GAAGA 7 cut(s) 203, 210, 314, 518, 743, 1322, 1558
MfeI CAATTG 1 cut(s) 969
MflI RGATCY 2 cut(s) 20, 88
MhlI GDGCHC 1 cut(s) 1081
MlyI GAGTC 1 cut(s) 603
MmeI TCCRAC 1 cut(s) 312
MnlI CCTC 3 cut(s) 194, 543, 1306
MroXI GAANNNNTTC 1 cut(s) 1553
MseI TTAA 9 cut(s) 386, 399, 458, 527, 552, 935, 1146, 1469, 1542
MslI CAYNNNNRTG 1 cut(s) 486
MspA1I CMGCKG 1 cut(s) 770
MspCI CTTAAG 2 cut(s) 526, 1541
MspI CCGG 4 cut(s) 668, 705, 1100, 1329
MspR9I CCNGG 2 cut(s) 135, 669
MssI GTTTAAAC 1 cut(s) 1470
MunI CAATTG 1 cut(s) 969
MvaI CCWGG 1 cut(s) 135
MwoI GCNNNNNNNGC 1 cut(s) 764
NciI CCSGG 1 cut(s) 669
NdeI CATATG 1 cut(s) 212
NlaIII CATG 7 cut(s) 360, 485, 643, 764, 964, 1325, 1416
NlaIV GGNNCC 2 cut(s) 90, 930
NmuCI GTSAC 3 cut(s) 469, 944, 1352
PaeR7I CTCGAG 1 cut(s) 1122
PagI TCATGA 1 cut(s) 1321
PdmI GAANNNNTTC 1 cut(s) 1553
PfeI GAWTC 4 cut(s) 345, 713, 860, 1118
PinAI ACCGGT 2 cut(s) 704, 1328
PkrI GCNGC 6 cut(s) 79, 677, 769, 883, 1174, 1375
PleI GAGTC 1 cut(s) 602
PmeI GTTTAAAC 1 cut(s) 1470
PpsI GAGTC 1 cut(s) 602
Ppu21I YACGTR 2 cut(s) 1111, 1477
PsiI TTATAA 3 cut(s) 576, 1485, 1579
Psp1406I AACGTT 1 cut(s) 383
Psp6I CCWGG 1 cut(s) 133
PspGI CCWGG 1 cut(s) 133
PspN4I GGNNCC 2 cut(s) 90, 930
PspPI GGNCC 5 cut(s) 203, 360, 928, 1163, 1315
PstNI CAGNNNCTG 1 cut(s) 158
PsuI RGATCY 2 cut(s) 20, 88
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 4 cut(s) 658, 666, 782, 1155
RsaNI GTAC 4 cut(s) 657, 665, 781, 1154
RseI CAYNNNNRTG 1 cut(s) 486
SaqAI TTAA 9 cut(s) 386, 399, 458, 527, 552, 935, 1146, 1469, 1542
SatI GCNGC 6 cut(s) 78, 676, 768, 882, 1173, 1374
Sau96I GGNCC 5 cut(s) 203, 360, 928, 1163, 1315
ScaI AGTACT 1 cut(s) 1155
SchI GAGTC 1 cut(s) 603
ScrFI CCNGG 2 cut(s) 135, 669
SduI GDGCHC 1 cut(s) 1081
SfaNI GCATC 3 cut(s) 170, 325, 868
SfcI CTRYAG 2 cut(s) 771, 1567
Sfr274I CTCGAG 1 cut(s) 1122
SgrAI CRCCGGYG 1 cut(s) 704
SinI GGWCC 4 cut(s) 203, 360, 928, 1163
SlaI CTCGAG 1 cut(s) 1122
SmiMI CAYNNNNRTG 1 cut(s) 486
SmlI CTYRAG 3 cut(s) 526, 1122, 1541
SmoI CTYRAG 3 cut(s) 526, 1122, 1541
SnaBI TACGTA 1 cut(s) 1111
SsiI CCGC 3 cut(s) 292, 563, 1373
SspI AATATT 1 cut(s) 14
SspMI CTAG 2 cut(s) 446, 1062
StyD4I CCNGG 2 cut(s) 133, 667
StyI CCWWGG 1 cut(s) 445
TaaI ACNGT 3 cut(s) 125, 1456, 1571
TaiI ACGT 4 cut(s) 273, 386, 1113, 1479
TaqI TCGA 4 cut(s) 435, 543, 592, 1123
TatI WGTACW 2 cut(s) 780, 1153
TauI GCSGC 1 cut(s) 1376
TfiI GAWTC 4 cut(s) 345, 713, 860, 1118
Tru1I TTAA 9 cut(s) 386, 399, 458, 527, 552, 935, 1146, 1469, 1542
Tru9I TTAA 9 cut(s) 386, 399, 458, 527, 552, 935, 1146, 1469, 1542
TscAI CASTG 4 cut(s) 12, 608, 1459, 1529
TseFI GTSAC 3 cut(s) 469, 944, 1352
TseI GCWGC 5 cut(s) 77, 675, 767, 881, 1172
Tsp45I GTSAC 3 cut(s) 469, 944, 1352
TspDTI ATGAA 9 cut(s) 130, 189, 332, 345, 453, 470, 525, 1310, 1563
TspGWI ACGGA 2 cut(s) 279, 1038
TspRI CASTG 4 cut(s) 12, 608, 1459, 1529
Vha464I CTTAAG 2 cut(s) 526, 1541
VneI GTGCAC 1 cut(s) 1077
VpaK11BI GGWCC 4 cut(s) 203, 360, 928, 1163
XapI RAATTY 6 cut(s) 107, 581, 992, 1206, 1534, 1553
XhoI CTCGAG 1 cut(s) 1122
XmaJI CCTAGG 1 cut(s) 445
XmnI GAANNNNTTC 1 cut(s) 1553
XspI CTAG 2 cut(s) 446, 1062
ZrmI AGTACT 1 cut(s) 1155
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.