Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr05

Orthogroup ID

OG0000117

Physical Location & Seq

Genomic Coordinates Reverse (-)

21914306 .. 21917664

Gene Structure 3359 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD05G1105800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1548 bp

ATGGCAATGAAATATTCACGACCTTTGCTCTTTGGTGTGCTGCTACTCATTGGCTTTGCATTGACAAATGGCAAAGCAACTAATACAGATCCACCTGTTCATTGCGAATTTCTCAATCGTAGCAGTTTTGAACCAGGGTTCATATTTGGCACAGGTTCCGCATCTTACCAGTATGAAGGTGCAGTAAAAGAAGATGGAAGAGGACCAAGCATATGGGATACCTTCACCCACAAACATCCAGAAAAAATCGCTGATGGCAGTAACGGAGACGTCGCTATTGATCAATATCACCGTTATAAGGAAGATGTGGGGATTATGAAGGATATGAATTTGGATGCTTACAGATTATCTATTTCATGGTCCAGATTATTACCAAATGGAACGTTAAGTGGTGGCGTTAACAGGAAAGGAATTGATTATTACAACAATCTCATCAATGAACTCCTACGCAATGGTTTAAAGCCGTTTGTAACACTCTTTCATTGGGATGTTCCCCAAGCTTTAGAAGATGAATATGGTGGTTTCTTAAGCCCTCGTATTGTAGATCATTTTAAAGACTATGCAGAACTTTGTTATAAGGAATTTGGTGATCGAGTCAAGCACTGGTTCACGGTAAATGAGCCATATACTTTTAGTAGCATGGGTTATGCTGTTGGGACTCTAGCACCGGGACGCTGCTCTTCTTGGCAAAACCTAAACTGCACCGGTGGAGATTCAGCCATTGAACCATACTTGGTGACACACCACATTCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAGGAATAGATATCAGGCATCTCAGAAAGGCTTGATAGGGATAACATTGGTGTCATACTGGTTTGAGCCTGCTTCGGAGTCAAAGCAAGATAAAGATGCTGCCTTACGATCTTTGGATTTTATGTTTGGATGGTTTTTGGACCCTTTAACAAGCGGTGACTATCCACACAGCATGCGATCAATTGTTGGAAAAAGATTGCCAAAATTCACAAAGGAACAATCCAAGTCGCTAAACGGATCATTTGATTTTCTTGGAATAAATTATTATACTGCTAGATACACAACTAGTACACCTAAGAACAATTCACTACAGGCAAGCTTCGTAACAGATCCTCGAGCTGATCTTACAACTGAGCTTAATGGAGTACTTATTGGTCCACAGACTGCTTCAGATTGGTTATATGTATATCCAAAAGGAATTCACGATCTTGTGCTCTACACAAAGGAAAAATATAATGATCCACTCATTTATATTACTGAGAATGGTGTATCAGAGTCGAATAATCCAAAACTATCAATTGACGAGGCTCTTCTTGATACCGGTAGAATTGACTACCACTACCGTCACCTATGTTACCTTCAAGTGGCGATCAAAAATGGTGCGAAAGTGAAGGGATACTTTCCATGGACATTGTTGGACGACTTTGAATGGAATTCTGGATACACCGTCCGATTTGGTTTAAACTACGTGGATTATAATGATGGGCTGAAAAGACACCCAAAACTCTCAGCACATTGGTTCAAAAATTTGCTTAAGAAGAATTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0004553 GO:0008150 GO:0008152 GO:0008422 GO:0015926 GO:0016787 GO:0016798 GO:0071704 GO:1901135 GO:1901657

KEGG Pathways

Metabolic & Signaling

ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110

Pfam Domains

Protein Families

Glyco_hydro_1

Protein Analysis

516

Amino Acids

58.49

Weight (kDa)

6.6

Isoelectric Point (pI)

23.29

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Glyco_hydro_1	PF00232	41 - 515	1.1e-155	Glycosyl hydrolase family 1

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

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Tree File

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Publication-ready

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Orthologous Genes (Group: OG0000117)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca	FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica	MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica	Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis	pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis	RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata	RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora	Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii	Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa	Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha	Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana	Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	3 cut(s)	297, 576, 1479
AatII	GACGTC	1 cut(s)	273
AciI	CCGC	2 cut(s)	159, 936
AclI	AACGTT	1 cut(s)	383
AclWI	GGATC	4 cut(s)	83, 1027, 1106, 1235
AcsI	RAATTY	7 cut(s)	107, 328, 581, 986, 1200, 1435, 1528
AcuI	CTGAAG	1 cut(s)	1155
AcyI	GRCGYC	1 cut(s)	270
AfaI	GTAC	3 cut(s)	782, 1072, 1149
AfiI	CCNNNNNNNGG	2 cut(s)	298, 1366
AflII	CTTAAG	2 cut(s)	526, 1535
AgeI	ACCGGT	2 cut(s)	704, 1322
AgsI	TTSAA	5 cut(s)	131, 725, 1364, 1430, 1525
AhlI	ACTAGT	1 cut(s)	1067
AjnI	CCWGG	1 cut(s)	133
AluBI	AGCT	5 cut(s)	500, 770, 1101, 1121, 1138
AluI	AGCT	5 cut(s)	500, 770, 1101, 1121, 1138
Alw21I	GWGCWC	1 cut(s)	1218
Alw26I	GTCTC	1 cut(s)	261
AlwI	GGATC	4 cut(s)	83, 1027, 1106, 1235
Ama87I	CYCGRG	1 cut(s)	1116
ApeKI	GCWGC	4 cut(s)	40, 675, 767, 881
ApoI	RAATTY	7 cut(s)	107, 328, 581, 986, 1200, 1435, 1528
AsiGI	ACCGGT	2 cut(s)	704, 1322
AspS9I	GGNCC	4 cut(s)	203, 360, 922, 1157
AsuC2I	CCSGG	1 cut(s)	669
AsuHPI	GGTGA	6 cut(s)	217, 281, 599, 748, 950, 1340
AvaI	CYCGRG	1 cut(s)	1116
AvaII	GGWCC	4 cut(s)	203, 360, 922, 1157
Bbv12I	GWGCWC	1 cut(s)	1218
BbvI	GCAGC	4 cut(s)	27, 662, 779, 868
BccI	CCATC	4 cut(s)	188, 248, 906, 1478
BceAI	ACGGC	1 cut(s)	448
BciT130I	CCWGG	1 cut(s)	135
BciVI	GTATCC	3 cut(s)	211, 1391, 1436
BclI	TGATCA	1 cut(s)	280
BcnI	CCSGG	1 cut(s)	669
BcoDI	GTCTC	1 cut(s)	261
BcuI	ACTAGT	1 cut(s)	1067
BfaI	CTAG	3 cut(s)	662, 1056, 1068
BfmI	CTRYAG	2 cut(s)	771, 1091
BfrI	CTTAAG	2 cut(s)	526, 1535
BfuI	GTATCC	3 cut(s)	211, 1391, 1436
BisI	GCNGC	4 cut(s)	41, 676, 768, 882
BlsI	GCNGC	4 cut(s)	42, 677, 769, 883
BmcAI	AGTACT	1 cut(s)	1149
Bme1390I	CCNGG	2 cut(s)	135, 669
Bme18I	GGWCC	4 cut(s)	203, 360, 922, 1157
BmeT110I	CYCGRG	1 cut(s)	1116
BmgT120I	GGNCC	4 cut(s)	203, 360, 922, 1157
BmiI	GGNNCC	2 cut(s)	157, 924
BmrFI	CCNGG	2 cut(s)	135, 669
BmsI	GCATC	4 cut(s)	170, 325, 809, 868
BpuMI	CCSGG	1 cut(s)	669
BsaAI	YACGTR	1 cut(s)	1471
BsaBI	GATNNNNATC	1 cut(s)	285
BsaHI	GRCGYC	1 cut(s)	270
BsaJI	CCNNGG	2 cut(s)	134, 1406
BsaWI	WCCGGW	2 cut(s)	704, 1322
BsaXI	ACNNNNNCTCC	2 cut(s)	756, 786
Bsc4I	CCNNNNNNNGG	2 cut(s)	298, 1366
Bse118I	RCCGGY	2 cut(s)	704, 1322
Bse1I	ACTGG	3 cut(s)	169, 608, 845
Bse3DI	GCAATG	3 cut(s)	12, 100, 457
Bse8I	GATNNNNATC	1 cut(s)	285
BseBI	CCWGG	1 cut(s)	135
BseDI	CCNNGG	2 cut(s)	134, 1406
BseGI	GGATG	4 cut(s)	235, 340, 493, 917
BseJI	GATNNNNATC	1 cut(s)	285
BseLI	CCNNNNNNNGG	2 cut(s)	298, 1366
BseMI	GCAATG	3 cut(s)	12, 100, 457
BseMII	CTCAG	4 cut(s)	818, 1125, 1251, 1524
BseNI	ACTGG	3 cut(s)	169, 608, 845
BseXI	GCAGC	4 cut(s)	27, 662, 779, 868
BsgI	GTGCAG	2 cut(s)	201, 685
BshTI	ACCGGT	2 cut(s)	704, 1322
BsiHKAI	GWGCWC	1 cut(s)	1218
BsiHKCI	CYCGRG	1 cut(s)	1116
BsiSI	CCGG	3 cut(s)	668, 705, 1323
BslFI	GGGAC	2 cut(s)	670, 684
BslI	CCNNNNNNNGG	2 cut(s)	298, 1366
BsmAI	GTCTC	1 cut(s)	261
BsmBI	CGTCTC	1 cut(s)	261
BsmFI	GGGAC	2 cut(s)	670, 684
BsoBI	CYCGRG	1 cut(s)	1116
Bsp1286I	GDGCHC	1 cut(s)	1218
Bsp1407I	TGTACA	1 cut(s)	780
Bsp19I	CCATGG	1 cut(s)	1406
BspACI	CCGC	2 cut(s)	159, 936
BspCNI	CTCAG	4 cut(s)	817, 1126, 1252, 1523
BspLI	GGNNCC	2 cut(s)	157, 924
BspPI	GGATC	4 cut(s)	83, 1027, 1106, 1235
BspQI	GCTCTTC	2 cut(s)	685, 1317
BspTI	CTTAAG	2 cut(s)	526, 1535
BsrDI	GCAATG	3 cut(s)	12, 100, 457
BsrFI	RCCGGY	2 cut(s)	704, 1322
BsrGI	TGTACA	1 cut(s)	780
BsrI	ACTGG	3 cut(s)	169, 608, 845
BssAI	RCCGGY	2 cut(s)	704, 1322
BssECI	CCNNGG	2 cut(s)	134, 1406
BssNI	GRCGYC	1 cut(s)	270
BssT1I	CCWWGG	1 cut(s)	1406
Bst2UI	CCWGG	1 cut(s)	135
Bst4CI	ACNGT	4 cut(s)	293, 613, 1346, 1450
Bst6I	CTCTTC	3 cut(s)	193, 685, 1317
BstACI	GRCGYC	1 cut(s)	270
BstAFI	CTTAAG	2 cut(s)	526, 1535
BstAUI	TGTACA	1 cut(s)	780
BstBAI	YACGTR	1 cut(s)	1471
BstC8I	GCNNGC	3 cut(s)	852, 956, 1099
BstDEI	CTNAG	5 cut(s)	804, 1077, 1134, 1260, 1510
BstDSI	CCRYGG	1 cut(s)	1406
BstF5I	GGATG	4 cut(s)	235, 340, 493, 917
BstMAI	GTCTC	1 cut(s)	261
BstMWI	GCNNNNNNNGC	1 cut(s)	764
BstNI	CCWGG	1 cut(s)	135
BstNSI	RCATGY	1 cut(s)	958
BstSCI	CCNGG	2 cut(s)	133, 667
BstSFI	CTRYAG	2 cut(s)	771, 1091
BstV1I	GCAGC	4 cut(s)	27, 662, 779, 868
BstX2I	RGATCY	2 cut(s)	88, 1111
BstXI	CCANNNNNNTGG	1 cut(s)	213
BstYI	RGATCY	2 cut(s)	88, 1111
BsuI	GTATCC	3 cut(s)	211, 1391, 1436
BtgI	CCRYGG	1 cut(s)	1406
BtsCI	GGATG	4 cut(s)	235, 340, 493, 917
BtsIMutI	CAGTG	1 cut(s)	601
Cac8I	GCNNGC	3 cut(s)	852, 956, 1099
Cfr10I	RCCGGY	2 cut(s)	704, 1322
Cfr13I	GGNCC	4 cut(s)	203, 360, 922, 1157
CseI	GACGC	1 cut(s)	681
Csp6I	GTAC	3 cut(s)	781, 1071, 1148
CspAI	ACCGGT	2 cut(s)	704, 1322
CviAII	CATG	5 cut(s)	357, 640, 761, 955, 1407
CviQI	GTAC	3 cut(s)	781, 1071, 1148
DdeI	CTNAG	5 cut(s)	804, 1077, 1134, 1260, 1510
DraI	TTTAAA	3 cut(s)	459, 553, 1464
Eam1104I	CTCTTC	3 cut(s)	193, 685, 1317
EarI	CTCTTC	3 cut(s)	193, 685, 1317
Eco130I	CCWWGG	1 cut(s)	1406
Eco32I	GATATC	1 cut(s)	794
Eco47I	GGWCC	4 cut(s)	203, 360, 922, 1157
Eco57I	CTGAAG	1 cut(s)	1155
Eco88I	CYCGRG	1 cut(s)	1116
EcoRI	GAATTC	2 cut(s)	1200, 1435
EcoRII	CCWGG	1 cut(s)	133
EcoRV	GATATC	1 cut(s)	794
EcoT14I	CCWWGG	1 cut(s)	1406
ErhI	CCWWGG	1 cut(s)	1406
Esp3I	CGTCTC	1 cut(s)	261
FaeI	CATG	5 cut(s)	360, 643, 764, 958, 1410
FalI	AAGNNNNNCTT	2 cut(s)	1385, 1417
FaqI	GGGAC	2 cut(s)	670, 684
FatI	CATG	5 cut(s)	356, 639, 760, 954, 1406
FauNDI	CATATG	1 cut(s)	212
FbaI	TGATCA	1 cut(s)	280
Fnu4HI	GCNGC	4 cut(s)	41, 676, 768, 882
FokI	GGATG	4 cut(s)	222, 347, 500, 924
Fsp4HI	GCNGC	4 cut(s)	41, 676, 768, 882
FspBI	CTAG	3 cut(s)	662, 1056, 1068
GluI	GCNGC	4 cut(s)	41, 676, 768, 882
HapII	CCGG	3 cut(s)	668, 705, 1323
HgaI	GACGC	1 cut(s)	681
Hin1I	GRCGYC	1 cut(s)	270
Hin1II	CATG	5 cut(s)	360, 643, 764, 958, 1410
HincII	GTYRAC	1 cut(s)	400
HindII	GTYRAC	1 cut(s)	400
HindIII	AAGCTT	2 cut(s)	498, 1099
HinfI	GANTC	5 cut(s)	594, 658, 713, 860, 1277
HpaI	GTTAAC	1 cut(s)	400
HpaII	CCGG	3 cut(s)	668, 705, 1323
HphI	GGTGA	6 cut(s)	217, 281, 599, 748, 950, 1340
Hpy166II	GTNNAC	4 cut(s)	400, 609, 1073, 1160
Hpy188I	TCNGA	5 cut(s)	807, 859, 1174, 1276, 1454
Hpy188III	TCNNGA	6 cut(s)	18, 239, 363, 1205, 1316, 1440
Hpy8I	GTNNAC	4 cut(s)	400, 609, 1073, 1160
Hpy99I	CGWCG	1 cut(s)	275
HpyAV	CCTTC	5 cut(s)	170, 232, 313, 1370, 1387
HpyCH4III	ACNGT	4 cut(s)	293, 613, 1346, 1450
HpyCH4IV	ACGT	3 cut(s)	270, 383, 1470
HpyCH4V	TGCA	4 cut(s)	59, 182, 563, 702
HpyF10VI	GCNNNNNNNGC	1 cut(s)	764
HpyF3I	CTNAG	5 cut(s)	804, 1077, 1134, 1260, 1510
HpySE526I	ACGT	3 cut(s)	270, 383, 1470
Hsp92I	GRCGYC	1 cut(s)	270
Hsp92II	CATG	5 cut(s)	360, 643, 764, 958, 1410
Ksp22I	TGATCA	1 cut(s)	280
KspAI	GTTAAC	1 cut(s)	400
LguI	GCTCTTC	2 cut(s)	685, 1317
LmnI	GCTCC	1 cut(s)	764
Lsp1109I	GCAGC	4 cut(s)	27, 662, 779, 868
LweI	GCATC	4 cut(s)	170, 325, 809, 868
MaeI	CTAG	3 cut(s)	662, 1056, 1068
MaeII	ACGT	3 cut(s)	270, 383, 1470
MaeIII	GTNAC	7 cut(s)	260, 469, 736, 938, 1105, 1346, 1355
MboII	GAAGA	7 cut(s)	203, 210, 314, 518, 672, 743, 1304
MfeI	CAATTG	2 cut(s)	963, 1299
MflI	RGATCY	2 cut(s)	88, 1111
MhlI	GDGCHC	1 cut(s)	1218
MlyI	GAGTC	4 cut(s)	603, 652, 869, 1286
MmeI	TCCRAC	2 cut(s)	949, 1398
MnlI	CCTC	4 cut(s)	194, 543, 1125, 1300
MseI	TTAA	9 cut(s)	386, 399, 458, 527, 552, 929, 1140, 1463, 1536
MslI	CAYNNNNRTG	1 cut(s)	486
MspA1I	CMGCKG	1 cut(s)	770
MspCI	CTTAAG	2 cut(s)	526, 1535
MspI	CCGG	3 cut(s)	668, 705, 1323
MspR9I	CCNGG	2 cut(s)	135, 669
MssI	GTTTAAAC	1 cut(s)	1464
MunI	CAATTG	2 cut(s)	963, 1299
MvaI	CCWGG	1 cut(s)	135
MwoI	GCNNNNNNNGC	1 cut(s)	764
NciI	CCSGG	1 cut(s)	669
NcoI	CCATGG	1 cut(s)	1406
NdeI	CATATG	1 cut(s)	212
NlaIII	CATG	5 cut(s)	360, 643, 764, 958, 1410
NlaIV	GGNNCC	2 cut(s)	157, 924
NmuCI	GTSAC	3 cut(s)	736, 938, 1346
NspI	RCATGY	1 cut(s)	958
PaeI	GCATGC	1 cut(s)	958
PaeR7I	CTCGAG	1 cut(s)	1116
PciSI	GCTCTTC	2 cut(s)	685, 1317
PfeI	GAWTC	1 cut(s)	713
PinAI	ACCGGT	2 cut(s)	704, 1322
PkrI	GCNGC	4 cut(s)	42, 677, 769, 883
PleI	GAGTC	4 cut(s)	602, 652, 868, 1285
PmeI	GTTTAAAC	1 cut(s)	1464
PpsI	GAGTC	4 cut(s)	602, 652, 868, 1285
Ppu21I	YACGTR	1 cut(s)	1471
PsiI	TTATAA	3 cut(s)	297, 576, 1479
Psp1406I	AACGTT	1 cut(s)	383
Psp6I	CCWGG	1 cut(s)	133
PspGI	CCWGG	1 cut(s)	133
PspN4I	GGNNCC	2 cut(s)	157, 924
PspPI	GGNCC	4 cut(s)	203, 360, 922, 1157
PspXI	VCTCGAGB	1 cut(s)	1116
PsuI	RGATCY	2 cut(s)	88, 1111
PvuII	CAGCTG	1 cut(s)	770
RsaI	GTAC	3 cut(s)	782, 1072, 1149
RsaNI	GTAC	3 cut(s)	781, 1071, 1148
RseI	CAYNNNNRTG	1 cut(s)	486
SapI	GCTCTTC	2 cut(s)	685, 1317
SaqAI	TTAA	9 cut(s)	386, 399, 458, 527, 552, 929, 1140, 1463, 1536
SatI	GCNGC	4 cut(s)	41, 676, 768, 882
Sau96I	GGNCC	4 cut(s)	203, 360, 922, 1157
ScaI	AGTACT	1 cut(s)	1149
SchI	GAGTC	4 cut(s)	603, 652, 869, 1286
ScrFI	CCNGG	2 cut(s)	135, 669
SduI	GDGCHC	1 cut(s)	1218
SfaNI	GCATC	4 cut(s)	170, 325, 809, 868
SfcI	CTRYAG	2 cut(s)	771, 1091
Sfr274I	CTCGAG	1 cut(s)	1116
SgrAI	CRCCGGYG	1 cut(s)	704
SinI	GGWCC	4 cut(s)	203, 360, 922, 1157
SlaI	CTCGAG	1 cut(s)	1116
SmiMI	CAYNNNNRTG	1 cut(s)	486
SmlI	CTYRAG	3 cut(s)	526, 1116, 1535
SmoI	CTYRAG	3 cut(s)	526, 1116, 1535
SpeI	ACTAGT	1 cut(s)	1067
SphI	GCATGC	1 cut(s)	958
SsiI	CCGC	2 cut(s)	159, 936
SspI	AATATT	1 cut(s)	14
SspMI	CTAG	3 cut(s)	662, 1056, 1068
StyD4I	CCNGG	2 cut(s)	133, 667
StyI	CCWWGG	1 cut(s)	1406
TaaI	ACNGT	4 cut(s)	293, 613, 1346, 1450
TaiI	ACGT	3 cut(s)	273, 386, 1473
TaqI	TCGA	3 cut(s)	592, 1117, 1280
TatI	WGTACW	3 cut(s)	780, 1070, 1147
TfiI	GAWTC	1 cut(s)	713
Tru1I	TTAA	9 cut(s)	386, 399, 458, 527, 552, 929, 1140, 1463, 1536
Tru9I	TTAA	9 cut(s)	386, 399, 458, 527, 552, 929, 1140, 1463, 1536
TscAI	CASTG	1 cut(s)	608
TseFI	GTSAC	3 cut(s)	736, 938, 1346
TseI	GCWGC	4 cut(s)	40, 675, 767, 881
Tsp45I	GTSAC	3 cut(s)	736, 938, 1346
TspGWI	ACGGA	2 cut(s)	279, 1032
TspRI	CASTG	1 cut(s)	608
Vha464I	CTTAAG	2 cut(s)	526, 1535
VpaK11BI	GGWCC	4 cut(s)	203, 360, 922, 1157
XapI	RAATTY	7 cut(s)	107, 328, 581, 986, 1200, 1435, 1528
XceI	RCATGY	1 cut(s)	958
XhoI	CTCGAG	1 cut(s)	1116
XspI	CTAG	3 cut(s)	662, 1056, 1068
ZraI	GACGTC	1 cut(s)	271
ZrmI	AGTACT	1 cut(s)	1149

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.