MD03G1068100.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000117
Physical Location & Seq
Reverse (-)
5455295 .. 5458574
3280 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1068100.v1.1.491

Sequence Viewer

Length: 1542 bp
ATGGCAATGAGGTTACAGTCACTGCTGTTGGGTGTGCTACTACTAACTGGCTTTGCAGCGGGAGATAGTAAAACTACTGCTGTTATACCTAGTCGTTATAGCAGTGCTTCCCTGAACAGAAGCAGTTTTCCATCAGGCTTTGTATTTGGTTCAGCTTCAGCATCTTACCAATATGAAGGTGCATGGGATGAAGGTGGTAAAGGACCAAGCATATGGGATAACTTCACCCACCAGTATCCAGAAAAAATCATTGATGGAAGCAATGGGGACGTGGCTGATGATCAATACCACCGCTATAAGGAAGATGTAAAGATTTTGAAGGATATGGGATTGGATGCTTATAGGTTCTCTATCTCATGGTCCAGATTGTTACCAAATGGAAAGCTAAGTGGGGGTGTGAACAAGGAAGGAGTACAATACTACAACAATCTCATCAATGAACTCCTAAACAAAGGTGTAACGCCATATGCGACAATCTTTCATTGGGATCTTCCTCAAGCTTTAGAAGAAGAATATGGTGGTTTCTTAAACCGTCAAATTGTCAATCATTTTCGAGACTACGCAGAACTTTGCTTTAAGTTATTTGGCGATCGGGTAAAGCATTGGATCACACTAAATGAGCCATATACTTTTATTACTTTTGGCTATGCATCAGGAGAATTAGCACCCGGGCGATGTTCTGCTTGGCAGAACTTAAACTGCACCGGCGGTGATTCGGCTACCGAACCCTATATAGTAGCACACCACTTCCTCCTCGCTCATGCACATGCTGTTAAAGTGTACAAGACTAAATATCAGGCATCTCAAGAAGGCGTGATAGGAATAACATTAGCGACAAATTGGTTTGTGCCGGTTTCTAACGCAACGCGTCATCGGAATGCTGCAAATCGATCTTTGGATTTTATGTTTGGATGGTTTATGGAGCCATTGACAAGCGGCCAATATCCGCACAGTATGCAAGTTCTTGTTAAAGAAAGATTACCTAAATTTACACAAGAAGAATCCAAGTTAATAAAAGGGTCATTTGATTTTGTTGGAATGAATTATTATACTACTCACTATTCAAGCGATCAACCTCATAATAATTCTGCAAATGCAAGCTTCCTGACCGATGCTCGCGTTTTTGAATCAACCGAGCTTAATGGAGTCCCCATTGGTCCTCCGGCTGCTTCAAGCTGGCTAGTTATTTATCCAAAAGGCATTCGAGAGATTTTACTCTACGCAAAGCACAAATATAATAATCCGCTCATTTACATTACTGAGAACGGCCTTGACGAGTTCGATGATCCCACATTGTCACTTCCGCAATCCCTCAATGATACCCACAGAATTGATTACCACTACCACCACCTCGACTATCTTCGAAAAGCAATCAATGATGGTGTAAATGTGAAGGGATACTTTGCATGGTCATTGCTGGACAATTTTGAATGGGCTTCTGGATACACCTTACGATTTGGTTTCGTCTATATAGATTACAATGATGGACTTAAGAGGCACCCAAAACTCTCAGCAAGCTGGTTCAAATGTTTCCTTGGATAA

Protein Analysis

514

Amino Acids

58.14

Weight (kDa)

6.34

Isoelectric Point (pI)

30.83

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 41 - 509 1.1e-162 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 1 cut(s) 1497
AccBSI CCGCTC 1 cut(s) 1246
AccII CGCG 2 cut(s) 868, 1119
AciI CCGC 7 cut(s) 59, 292, 708, 936, 947, 1244, 1304
AclWI GGATC 3 cut(s) 495, 614, 1280
AcoI YGGCCR 1 cut(s) 937
AcsI RAATTY 1 cut(s) 986
AcuI CTGAAG 1 cut(s) 141
AfaI GTAC 2 cut(s) 414, 782
AfiI CCNNNNNNNGG 1 cut(s) 298
AflII CTTAAG 1 cut(s) 1490
AflIII ACRYGT 1 cut(s) 866
AgsI TTSAA 6 cut(s) 319, 1065, 1127, 1173, 1430, 1525
AjiI CACGTC 1 cut(s) 271
AluBI AGCT 7 cut(s) 155, 385, 500, 1101, 1138, 1176, 1518
AluI AGCT 7 cut(s) 155, 385, 500, 1101, 1138, 1176, 1518
Alw26I GTCTC 1 cut(s) 549
AlwI GGATC 3 cut(s) 495, 614, 1280
AlwNI CAGNNNCTG 1 cut(s) 22
Ama87I CYCGRG 1 cut(s) 668
AoxI GGCC 2 cut(s) 937, 1267
ApeKI GCWGC 3 cut(s) 56, 881, 1166
ApoI RAATTY 1 cut(s) 986
AspS9I GGNCC 3 cut(s) 203, 360, 1157
AsuC2I CCSGG 2 cut(s) 669, 670
AsuHPI GGTGA 2 cut(s) 217, 722
AsuII TTCGAA 1 cut(s) 1363
AvaI CYCGRG 1 cut(s) 668
AvaII GGWCC 3 cut(s) 203, 360, 1157
BanI GGYRCC 1 cut(s) 1497
BbvI GCAGC 3 cut(s) 68, 868, 1153
BccI CCATC 5 cut(s) 139, 248, 906, 1373, 1478
BceAI ACGGC 1 cut(s) 1282
BciVI GTATCC 3 cut(s) 246, 1391, 1436
BclI TGATCA 1 cut(s) 280
BcnI CCSGG 2 cut(s) 669, 670
BcoDI GTCTC 1 cut(s) 549
BfaI CTAG 2 cut(s) 90, 1181
BfrI CTTAAG 1 cut(s) 1490
BfuI GTATCC 3 cut(s) 246, 1391, 1436
BisI GCNGC 4 cut(s) 57, 882, 937, 1167
BlsI GCNGC 4 cut(s) 58, 883, 938, 1168
Bme1390I CCNGG 2 cut(s) 669, 670
Bme18I GGWCC 3 cut(s) 203, 360, 1157
BmeT110I CYCGRG 1 cut(s) 668
BmgBI CACGTC 1 cut(s) 271
BmgT120I GGNCC 3 cut(s) 203, 360, 1157
BmiI GGNNCC 2 cut(s) 924, 1499
BmrFI CCNGG 2 cut(s) 669, 670
BmsI GCATC 5 cut(s) 170, 325, 659, 809, 1102
Bpu14I TTCGAA 1 cut(s) 1363
BpuEI CTTGAG 2 cut(s) 480, 789
BpuMI CCSGG 2 cut(s) 669, 670
Bsa29I ATCGAT 1 cut(s) 889
BsaJI CCNNGG 2 cut(s) 668, 1534
Bsc4I CCNNNNNNNGG 1 cut(s) 298
Bse118I RCCGGY 2 cut(s) 704, 850
Bse1I ACTGG 2 cut(s) 52, 232
Bse3DI GCAATG 3 cut(s) 12, 268, 1412
BseCI ATCGAT 1 cut(s) 889
BseDI CCNNGG 2 cut(s) 668, 1534
BseGI GGATG 3 cut(s) 193, 340, 917
BseLI CCNNNNNNNGG 1 cut(s) 298
BseMI GCAATG 3 cut(s) 12, 268, 1412
BseMII CTCAG 2 cut(s) 1251, 1524
BseNI ACTGG 2 cut(s) 52, 232
BseRI GAGGAG 1 cut(s) 743
BseXI GCAGC 3 cut(s) 68, 868, 1153
BsgI GTGCAG 1 cut(s) 685
Bsh1236I CGCG 2 cut(s) 868, 1119
Bsh1285I CGRYCG 1 cut(s) 592
BshFI GGCC 2 cut(s) 939, 1269
BshNI GGYRCC 1 cut(s) 1497
BshVI ATCGAT 1 cut(s) 889
BsiEI CGRYCG 1 cut(s) 592
BsiHKCI CYCGRG 1 cut(s) 668
BsiSI CCGG 4 cut(s) 669, 705, 851, 1163
BslFI GGGAC 2 cut(s) 281, 1133
BslI CCNNNNNNNGG 1 cut(s) 298
BsmAI GTCTC 1 cut(s) 549
BsmFI GGGAC 2 cut(s) 281, 1133
BsmI GAATGC 2 cut(s) 883, 1200
BsnI GGCC 2 cut(s) 939, 1269
BsoBI CYCGRG 1 cut(s) 668
Bsp119I TTCGAA 1 cut(s) 1363
Bsp1407I TGTACA 1 cut(s) 780
Bsp143I GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
BspACI CCGC 7 cut(s) 59, 292, 708, 936, 947, 1244, 1304
BspANI GGCC 2 cut(s) 939, 1269
BspCNI CTCAG 2 cut(s) 1252, 1523
BspDI ATCGAT 1 cut(s) 889
BspFNI CGCG 2 cut(s) 868, 1119
BspLI GGNNCC 2 cut(s) 924, 1499
BspPI GGATC 3 cut(s) 495, 614, 1280
BspT104I TTCGAA 1 cut(s) 1363
BspT107I GGYRCC 1 cut(s) 1497
BspTI CTTAAG 1 cut(s) 1490
BsrBI CCGCTC 1 cut(s) 1246
BsrDI GCAATG 3 cut(s) 12, 268, 1412
BsrFI RCCGGY 2 cut(s) 704, 850
BsrGI TGTACA 1 cut(s) 780
BsrI ACTGG 2 cut(s) 52, 232
BssAI RCCGGY 2 cut(s) 704, 850
BssECI CCNNGG 2 cut(s) 668, 1534
BssMI GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
BssT1I CCWWGG 1 cut(s) 1534
Bst4CI ACNGT 3 cut(s) 18, 533, 953
BstAFI CTTAAG 1 cut(s) 1490
BstAPI GCANNNNNTGC 1 cut(s) 955
BstAUI TGTACA 1 cut(s) 780
BstBI TTCGAA 1 cut(s) 1363
BstC8I GCNNGC 4 cut(s) 1099, 1117, 1178, 1516
BstDEI CTNAG 3 cut(s) 386, 1260, 1510
BstF5I GGATG 3 cut(s) 193, 340, 917
BstFNI CGCG 2 cut(s) 868, 1119
BstKTI GATC 7 cut(s) 283, 490, 592, 609, 893, 1072, 1288
BstMAI GTCTC 1 cut(s) 549
BstMBI GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
BstMCI CGRYCG 1 cut(s) 592
BstMWI GCNNNNNNNGC 1 cut(s) 955
BstNSI RCATGY 1 cut(s) 770
BstSCI CCNGG 2 cut(s) 667, 668
BstUI CGCG 2 cut(s) 868, 1119
BstV1I GCAGC 3 cut(s) 68, 868, 1153
BstX2I RGATCY 1 cut(s) 487
BstXI CCANNNNNNTGG 1 cut(s) 213
BstYI RGATCY 1 cut(s) 487
Bsu15I ATCGAT 1 cut(s) 889
BsuI GTATCC 3 cut(s) 246, 1391, 1436
BsuRI GGCC 2 cut(s) 939, 1269
BsuTUI ATCGAT 1 cut(s) 889
BtgZI GCGATG 1 cut(s) 688
BtrI CACGTC 1 cut(s) 271
BtsCI GGATG 3 cut(s) 193, 340, 917
BtsI GCAGTG 2 cut(s) 20, 109
BtsIMutI CAGTG 2 cut(s) 20, 109
Cac8I GCNNGC 4 cut(s) 1099, 1117, 1178, 1516
CaiI CAGNNNCTG 1 cut(s) 22
Cfr10I RCCGGY 2 cut(s) 704, 850
Cfr13I GGNCC 3 cut(s) 203, 360, 1157
Cfr9I CCCGGG 1 cut(s) 668
ClaI ATCGAT 1 cut(s) 889
CseI GACGC 1 cut(s) 857
Csp6I GTAC 2 cut(s) 413, 781
CviAII CATG 5 cut(s) 183, 357, 761, 767, 1407
CviQI GTAC 2 cut(s) 413, 781
DdeI CTNAG 3 cut(s) 386, 1260, 1510
DpnI GATC 7 cut(s) 282, 489, 591, 608, 892, 1071, 1287
DpnII GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
EaeI YGGCCR 1 cut(s) 937
Eco130I CCWWGG 1 cut(s) 1534
Eco47I GGWCC 3 cut(s) 203, 360, 1157
Eco57I CTGAAG 1 cut(s) 141
Eco88I CYCGRG 1 cut(s) 668
EcoT14I CCWWGG 1 cut(s) 1534
EcoT22I ATGCAT 1 cut(s) 652
ErhI CCWWGG 1 cut(s) 1534
FaeI CATG 5 cut(s) 186, 360, 764, 770, 1410
FalI AAGNNNNNCTT 2 cut(s) 1385, 1417
FaqI GGGAC 2 cut(s) 281, 1133
FatI CATG 5 cut(s) 182, 356, 760, 766, 1406
FauI CCCGC 1 cut(s) 52
FauNDI CATATG 2 cut(s) 212, 466
FbaI TGATCA 1 cut(s) 280
Fnu4HI GCNGC 4 cut(s) 57, 882, 937, 1167
FokI GGATG 3 cut(s) 200, 347, 924
Fsp4HI GCNGC 4 cut(s) 57, 882, 937, 1167
FspBI CTAG 2 cut(s) 90, 1181
GluI GCNGC 4 cut(s) 57, 882, 937, 1167
HaeIII GGCC 2 cut(s) 939, 1269
HapII CCGG 4 cut(s) 669, 705, 851, 1163
HgaI GACGC 1 cut(s) 857
Hin1II CATG 5 cut(s) 186, 360, 764, 770, 1410
HindIII AAGCTT 2 cut(s) 498, 1099
HinfI GANTC 4 cut(s) 713, 1001, 1127, 1146
HpaII CCGG 4 cut(s) 669, 705, 851, 1163
HphI GGTGA 2 cut(s) 217, 722
Hpy166II GTNNAC 2 cut(s) 400, 781
Hpy188I TCNGA 1 cut(s) 876
Hpy188III TCNNGA 8 cut(s) 239, 363, 554, 654, 806, 1105, 1205, 1440
Hpy8I GTNNAC 2 cut(s) 400, 781
HpyAV CCTTC 6 cut(s) 170, 185, 313, 401, 803, 1387
HpyCH4III ACNGT 3 cut(s) 18, 533, 953
HpyCH4IV ACGT 1 cut(s) 270
HpyF10VI GCNNNNNNNGC 1 cut(s) 955
HpyF3I CTNAG 3 cut(s) 386, 1260, 1510
HpySE526I ACGT 1 cut(s) 270
Hsp92II CATG 5 cut(s) 186, 360, 764, 770, 1410
Ksp22I TGATCA 1 cut(s) 280
Kzo9I GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
LmnI GCTCC 1 cut(s) 922
Lsp1109I GCAGC 3 cut(s) 68, 868, 1153
LweI GCATC 5 cut(s) 170, 325, 659, 809, 1102
MaeI CTAG 2 cut(s) 90, 1181
MaeII ACGT 1 cut(s) 270
MaeIII GTNAC 5 cut(s) 12, 18, 369, 457, 1296
MalI GATC 7 cut(s) 282, 489, 591, 608, 892, 1071, 1287
MbiI CCGCTC 1 cut(s) 1246
MboI GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
MboII GAAGA 6 cut(s) 314, 482, 518, 521, 1010, 1352
MflI RGATCY 1 cut(s) 487
MluCI AATT 8 cut(s) 537, 659, 838, 986, 1042, 1084, 1329, 1423
MluI ACGCGT 1 cut(s) 866
MlyI GAGTC 1 cut(s) 1155
MmeI TCCRAC 1 cut(s) 1015
MnlI CCTC 9 cut(s) 3, 504, 761, 764, 1086, 1170, 1322, 1361, 1488
Mph1103I ATGCAT 1 cut(s) 652
MseI TTAA 8 cut(s) 527, 576, 695, 774, 969, 1010, 1140, 1491
MslI CAYNNNNRTG 2 cut(s) 765, 876
MspA1I CMGCKG 1 cut(s) 59
MspCI CTTAAG 1 cut(s) 1490
MspI CCGG 4 cut(s) 669, 705, 851, 1163
MspR9I CCNGG 2 cut(s) 669, 670
Mva1269I GAATGC 2 cut(s) 883, 1200
MvnI CGCG 2 cut(s) 868, 1119
MwoI GCNNNNNNNGC 1 cut(s) 955
NciI CCSGG 2 cut(s) 669, 670
NdeI CATATG 2 cut(s) 212, 466
NdeII GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
NlaIII CATG 5 cut(s) 186, 360, 764, 770, 1410
NlaIV GGNNCC 2 cut(s) 924, 1499
NmuCI GTSAC 2 cut(s) 18, 1296
NsiI ATGCAT 1 cut(s) 652
NspI RCATGY 1 cut(s) 770
NspV TTCGAA 1 cut(s) 1363
PcsI WCGNNNNNNNCGW 2 cut(s) 467, 1272
PctI GAATGC 2 cut(s) 883, 1200
PfeI GAWTC 3 cut(s) 713, 1001, 1127
PkrI GCNGC 4 cut(s) 58, 883, 938, 1168
Ple19I CGATCG 1 cut(s) 592
PleI GAGTC 1 cut(s) 1154
PpsI GAGTC 1 cut(s) 1154
PspN4I GGNNCC 2 cut(s) 924, 1499
PspPI GGNCC 3 cut(s) 203, 360, 1157
PstNI CAGNNNCTG 1 cut(s) 22
PsuI RGATCY 1 cut(s) 487
PvuI CGATCG 1 cut(s) 592
RsaI GTAC 2 cut(s) 414, 782
RsaNI GTAC 2 cut(s) 413, 781
RseI CAYNNNNRTG 2 cut(s) 765, 876
SaqAI TTAA 8 cut(s) 527, 576, 695, 774, 969, 1010, 1140, 1491
SatI GCNGC 4 cut(s) 57, 882, 937, 1167
Sau3AI GATC 7 cut(s) 280, 487, 589, 606, 890, 1069, 1285
Sau96I GGNCC 3 cut(s) 203, 360, 1157
SchI GAGTC 1 cut(s) 1155
ScrFI CCNGG 2 cut(s) 669, 670
SfaNI GCATC 5 cut(s) 170, 325, 659, 809, 1102
SfuI TTCGAA 1 cut(s) 1363
SgrAI CRCCGGYG 1 cut(s) 704
SinI GGWCC 3 cut(s) 203, 360, 1157
SmaI CCCGGG 1 cut(s) 670
SmiMI CAYNNNNRTG 2 cut(s) 765, 876
SmlI CTYRAG 3 cut(s) 495, 804, 1490
SmoI CTYRAG 3 cut(s) 495, 804, 1490
Sse9I AATT 8 cut(s) 537, 659, 838, 986, 1042, 1084, 1329, 1423
SsiI CCGC 7 cut(s) 59, 292, 708, 936, 947, 1244, 1304
SspMI CTAG 2 cut(s) 90, 1181
StyD4I CCNGG 2 cut(s) 667, 668
StyI CCWWGG 1 cut(s) 1534
TaaI ACNGT 3 cut(s) 18, 533, 953
TaiI ACGT 1 cut(s) 273
TaqI TCGA 6 cut(s) 553, 889, 1204, 1281, 1353, 1363
TaqII GACCGA 1 cut(s) 1124
TasI AATT 8 cut(s) 537, 659, 838, 986, 1042, 1084, 1329, 1423
TatI WGTACW 2 cut(s) 412, 780
TauI GCSGC 1 cut(s) 939
TfiI GAWTC 3 cut(s) 713, 1001, 1127
Tru1I TTAA 8 cut(s) 527, 576, 695, 774, 969, 1010, 1140, 1491
Tru9I TTAA 8 cut(s) 527, 576, 695, 774, 969, 1010, 1140, 1491
TscAI CASTG 2 cut(s) 27, 109
TseFI GTSAC 2 cut(s) 18, 1296
TseI GCWGC 3 cut(s) 56, 881, 1166
Tsp45I GTSAC 2 cut(s) 18, 1296
TspDTI ATGAA 5 cut(s) 189, 204, 453, 470, 1055
TspMI CCCGGG 1 cut(s) 668
TspRI CASTG 2 cut(s) 27, 109
Vha464I CTTAAG 1 cut(s) 1490
VpaK11BI GGWCC 3 cut(s) 203, 360, 1157
XapI RAATTY 1 cut(s) 986
XceI RCATGY 1 cut(s) 770
XmaI CCCGGG 1 cut(s) 668
XspI CTAG 2 cut(s) 90, 1181
Zsp2I ATGCAT 1 cut(s) 652
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.