MD03G1098600.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000117
Physical Location & Seq
Reverse (-)
8435437 .. 8439582
4146 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1098600.v1.1.491

Sequence Viewer

Length: 1611 bp
ATGGCGATGCATGAGTTAGGATCTTTGCTCTTGTTTGTGTTGCTACTCACTGGCTTTGCATTGACAAACACCAAAGCTGCTAAGCCATATCGGCCCATTGTCTGTAACTCTCTTGACAGGGGAAAATTTGATGCTCTTAAACCAGGGTTCGTCTTTGGCGCAGCTTCAGCATCTTACCAGGTAGAAGGTGCATGGAACGAAGATGGTAGAGGACCAAGCATATGGGACACCTTCACCCACAACCATCCAGAAAAAATCACTGATCGCAGCAATGGAGATGTCGCCATTGATCAATACCACCTCTATAAGAAAGATGTAGCAATTATGAAGGATATGAAGTTCGATGCTTATAGGTTCTCTATCTCGTGGTCCAGATTGTTACCAAATGGAACGCTAAGTGGGGGTGTTAACAGGAAAGGAATTGAATATTACGACAATCTCATCAATGAACTCCTTCTCCATGGCCTACAACCATTTGTGACACTCTTTCACTGGGATGTTCCTCAAGCTTTAGAAGATGCATATGGTGGTTTCTTAAGCCCTCGTATTGCCGATGACTTTAAAGACTACGCAGAACTTTGTTTTTCACTTTTTGGTGACCGAGTGAAGCACTGGATCACGTTGAATGAGCCATATACCTTTAGCAACCATGCTTATACAATTGGGATCCACGCACCCGGACGATGCTCTGCTTGGCAAGACCCAACCTGCCTCGGTGGAGATTCAGCTATCGAACCCTATTTAGTCACACACCACCAACTTCTTGCTCATGCAGCTGCTGTAAAAGTGTACAAAGAAAATTTTCAGGCATATCAGAATGGGGTGATAGGAATAACACTAGTGTCGCACTGGTTTGAGCCTGCTTCAGAAGCAAAGGAAGATATGGATGCTTCATATCGAGCTTTGGATTTTATGTTCGGATGGTTTATGGATCCAATTACAAGAGGTGACTACCCATACAGCATGCAAGGCCTCGTTAGAGAACGATTACCCAAATTCACAGATGAAGAATCAAAGATGTTAATTGGGTCTTTTGACTTTATTGGACTGAACTATTATTCTGCTAGATATGCAGCTGATGTACCTAAGAATTATTCTGAACCTGCAAGTTACTTATATGATCCACATGTTACTTTACTAACTGAACTTGATGGCGTTCCTATTGGTCCTCAGGCTGCTTCAAACTGGTTATATGTTTATCCAAAAGGAATTCATGATTTTGTACTCTACACGAAGAATAAGTATGGTGATCCAATCATTTACATTACTGAGAATGGCATTGACGAGGTCAATAATTCAACCTTATCACTAGAGGATGCTCTTCATGACACCAATAGAACTGACTACTACAATCACCATCTCTGTTACCTTCAAGCAGCAATCAAGAAGGGAAGTAATGTGAAAGGATACTTTGCATGGTCAATTTTAGACAACTTTGAATGGAGCGAAGGGTATACAGTTCGATTTGGTATCAACTATGTGGATTATGACAATGGACGGAAAAGGCACCCAAAACTTTCGACGTATTGGTTCAAGAACTTCCTTAAGAAGCGCAAAAGAAATTATAATATTTTGGCCGATTATGTTGGAGACACTAAGTCTGTGTATTAA

Protein Analysis

537

Amino Acids

61.27

Weight (kDa)

5.88

Isoelectric Point (pI)

24.47

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 46 - 518 1.3e-158 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1566
Acc36I ACCTGC 2 cut(s) 716, 1111
AccB1I GGYRCC 1 cut(s) 1506
AccI GTMKAC 1 cut(s) 1454
AclWI GGATC 8 cut(s) 28, 623, 661, 674, 926, 939, 1115, 1244
AcoI YGGCCR 1 cut(s) 1575
AcsI RAATTY 4 cut(s) 125, 799, 995, 1209
AcuI CTGAAG 2 cut(s) 150, 849
AfaI GTAC 3 cut(s) 791, 1083, 1224
AflII CTTAAG 2 cut(s) 535, 1544
AflIII ACRYGT 1 cut(s) 1126
AgsI TTSAA 7 cut(s) 425, 625, 1182, 1299, 1373, 1439, 1534
AhdI GACNNNNNGTC 1 cut(s) 1597
AhlI ACTAGT 1 cut(s) 838
AjnI CCWGG 2 cut(s) 142, 177
AloI GAACNNNNNNTCC 6 cut(s) 323, 355, 441, 473, 899, 931
AluBI AGCT 7 cut(s) 77, 164, 509, 728, 776, 902, 1076
AluI AGCT 7 cut(s) 77, 164, 509, 728, 776, 902, 1076
Alw26I GTCTC 1 cut(s) 1584
AlwI GGATC 8 cut(s) 28, 623, 661, 674, 926, 939, 1115, 1244
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 4 cut(s) 92, 463, 970, 1575
ApeKI GCWGC 8 cut(s) 77, 161, 267, 773, 776, 1073, 1175, 1376
ApoI RAATTY 4 cut(s) 125, 799, 995, 1209
Asp700I GAANNNNTTC 2 cut(s) 453, 801
AspLEI GCGC 2 cut(s) 161, 1554
AspS9I GGNCC 4 cut(s) 93, 212, 369, 1166
AsuC2I CCSGG 1 cut(s) 678
AsuHPI GGTGA 6 cut(s) 226, 608, 835, 959, 1259, 1346
AvaII GGWCC 3 cut(s) 212, 369, 1166
AxyI CCTNAGG 1 cut(s) 1170
BamHI GGATCC 2 cut(s) 666, 931
BanI GGYRCC 1 cut(s) 1506
BauI CACGAG 1 cut(s) 364
BbvI GCAGC 8 cut(s) 64, 173, 279, 763, 785, 1085, 1162, 1388
BccI CCATC 5 cut(s) 197, 252, 915, 1145, 1365
BciT130I CCWGG 2 cut(s) 144, 179
BciVI GTATCC 1 cut(s) 1400
BclI TGATCA 1 cut(s) 289
BcnI CCSGG 1 cut(s) 678
BcoDI GTCTC 1 cut(s) 1584
BcuI ACTAGT 1 cut(s) 838
BfaI CTAG 3 cut(s) 839, 1065, 1310
BfrI CTTAAG 2 cut(s) 535, 1544
BfuAI ACCTGC 2 cut(s) 716, 1111
BfuI GTATCC 1 cut(s) 1400
BglI GCCNNNNNGGC 1 cut(s) 91
BisI GCNGC 8 cut(s) 78, 162, 268, 774, 777, 1074, 1176, 1377
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 8 cut(s) 79, 163, 269, 775, 778, 1075, 1177, 1378
Bme1390I CCNGG 3 cut(s) 144, 179, 678
Bme18I GGWCC 3 cut(s) 212, 369, 1166
BmeRI GACNNNNNGTC 1 cut(s) 1597
BmgT120I GGNCC 4 cut(s) 93, 212, 369, 1166
BmiI GGNNCC 3 cut(s) 668, 933, 1508
BmrFI CCNGG 3 cut(s) 144, 179, 678
BmrI ACTGGG 1 cut(s) 502
BmsI GCATC 7 cut(s) 121, 179, 334, 508, 674, 877, 1306
BmuI ACTGGG 1 cut(s) 502
Bpu1102I GCTNAGC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 489
BpuMI CCSGG 1 cut(s) 678
BsaJI CCNNGG 3 cut(s) 143, 460, 712
BsaXI ACNNNNNCTCC 2 cut(s) 441, 471
Bse1I ACTGG 5 cut(s) 55, 497, 617, 854, 1190
Bse21I CCTNAGG 1 cut(s) 1170
Bse3DI GCAATG 1 cut(s) 277
BseBI CCWGG 2 cut(s) 144, 179
BseDI CCNNGG 3 cut(s) 143, 460, 712
BseGI GGATG 5 cut(s) 244, 502, 892, 926, 1321
BseMI GCAATG 1 cut(s) 277
BseMII CTCAG 2 cut(s) 1184, 1260
BseNI ACTGG 5 cut(s) 55, 497, 617, 854, 1190
BseXI GCAGC 8 cut(s) 64, 173, 279, 763, 785, 1085, 1162, 1388
BshFI GGCC 4 cut(s) 94, 465, 972, 1577
BshNI GGYRCC 1 cut(s) 1506
BsiSI CCGG 1 cut(s) 678
BslFI GGGAC 1 cut(s) 239
BsmAI GTCTC 1 cut(s) 1584
BsmFI GGGAC 1 cut(s) 239
BsnI GGCC 4 cut(s) 94, 465, 972, 1577
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
Bsp1720I GCTNAGC 1 cut(s) 81
Bsp19I CCATGG 1 cut(s) 460
BspANI GGCC 4 cut(s) 94, 465, 972, 1577
BspCNI CTCAG 2 cut(s) 1183, 1261
BspHI TCATGA 2 cut(s) 1213, 1324
BspLI GGNNCC 3 cut(s) 668, 933, 1508
BspMI ACCTGC 2 cut(s) 716, 1111
BspPI GGATC 8 cut(s) 28, 623, 661, 674, 926, 939, 1115, 1244
BspQI GCTCTTC 1 cut(s) 1326
BspT107I GGYRCC 1 cut(s) 1506
BspTI CTTAAG 2 cut(s) 535, 1544
BsrDI GCAATG 1 cut(s) 277
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 5 cut(s) 55, 497, 617, 854, 1190
BssECI CCNNGG 3 cut(s) 143, 460, 712
BssMI GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
BssNAI GTATAC 1 cut(s) 1455
BssSI CACGAG 1 cut(s) 364
BssT1I CCWWGG 1 cut(s) 460
Bst1107I GTATAC 1 cut(s) 1455
Bst2BI CACGAG 1 cut(s) 364
Bst2UI CCWGG 2 cut(s) 144, 179
Bst4CI ACNGT 1 cut(s) 1459
Bst6I CTCTTC 1 cut(s) 1326
BstAFI CTTAAG 2 cut(s) 535, 1544
BstAUI TGTACA 1 cut(s) 789
BstC8I GCNNGC 2 cut(s) 861, 965
BstDEI CTNAG 6 cut(s) 81, 395, 1086, 1170, 1269, 1596
BstDSI CCRYGG 1 cut(s) 460
BstEII GGTNACC 1 cut(s) 596
BstF5I GGATG 5 cut(s) 244, 502, 892, 926, 1321
BstHHI GCGC 2 cut(s) 161, 1554
BstKTI GATC 8 cut(s) 23, 265, 292, 618, 669, 934, 1123, 1252
BstMAI GTCTC 1 cut(s) 1584
BstMBI GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
BstMWI GCNNNNNNNGC 6 cut(s) 91, 167, 773, 869, 969, 1070
BstNI CCWGG 2 cut(s) 144, 179
BstNSI RCATGY 2 cut(s) 967, 1130
BstPI GGTNACC 1 cut(s) 596
BstSCI CCNGG 3 cut(s) 142, 177, 676
BstV1I GCAGC 8 cut(s) 64, 173, 279, 763, 785, 1085, 1162, 1388
BstX2I RGATCY 3 cut(s) 20, 666, 931
BstXI CCANNNNNNTGG 1 cut(s) 222
BstYI RGATCY 3 cut(s) 20, 666, 931
BstZ17I GTATAC 1 cut(s) 1455
Bsu36I CCTNAGG 1 cut(s) 1170
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 4 cut(s) 94, 465, 972, 1577
BtgI CCRYGG 1 cut(s) 460
BtgZI GCGATG 1 cut(s) 20
BtsCI GGATG 5 cut(s) 244, 502, 892, 926, 1321
BtsIMutI CAGTG 5 cut(s) 48, 258, 490, 610, 847
BveI ACCTGC 2 cut(s) 716, 1111
Cac8I GCNNGC 2 cut(s) 861, 965
CaiI CAGNNNCTG 1 cut(s) 779
CciI TCATGA 2 cut(s) 1213, 1324
CfoI GCGC 2 cut(s) 161, 1554
Cfr13I GGNCC 4 cut(s) 93, 212, 369, 1166
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 3 cut(s) 790, 1082, 1223
CviQI GTAC 3 cut(s) 790, 1082, 1223
DdeI CTNAG 6 cut(s) 81, 395, 1086, 1170, 1269, 1596
DpnI GATC 8 cut(s) 22, 264, 291, 617, 668, 933, 1122, 1251
DpnII GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
DraI TTTAAA 1 cut(s) 562
DriI GACNNNNNGTC 1 cut(s) 1597
EaeI YGGCCR 1 cut(s) 1575
Eam1104I CTCTTC 1 cut(s) 1326
Eam1105I GACNNNNNGTC 1 cut(s) 1597
EarI CTCTTC 1 cut(s) 1326
Eco130I CCWWGG 1 cut(s) 460
Eco147I AGGCCT 1 cut(s) 972
Eco47I GGWCC 3 cut(s) 212, 369, 1166
Eco57I CTGAAG 2 cut(s) 150, 849
Eco81I CCTNAGG 1 cut(s) 1170
Eco91I GGTNACC 1 cut(s) 596
EcoO65I GGTNACC 1 cut(s) 596
EcoRI GAATTC 1 cut(s) 1209
EcoRII CCWGG 2 cut(s) 142, 177
EcoT14I CCWWGG 1 cut(s) 460
EcoT22I ATGCAT 2 cut(s) 12, 523
ErhI CCWWGG 1 cut(s) 460
FaqI GGGAC 1 cut(s) 239
FauNDI CATATG 2 cut(s) 221, 523
FbaI TGATCA 1 cut(s) 289
FblI GTMKAC 1 cut(s) 1454
Fnu4HI GCNGC 8 cut(s) 78, 162, 268, 774, 777, 1074, 1176, 1377
FokI GGATG 5 cut(s) 231, 509, 899, 933, 1328
Fsp4HI GCNGC 8 cut(s) 78, 162, 268, 774, 777, 1074, 1176, 1377
FspBI CTAG 3 cut(s) 839, 1065, 1310
GlaI GCGC 2 cut(s) 160, 1553
GluI GCNGC 8 cut(s) 78, 162, 268, 774, 777, 1074, 1176, 1377
HaeIII GGCC 4 cut(s) 94, 465, 972, 1577
HapII CCGG 1 cut(s) 678
HhaI GCGC 2 cut(s) 161, 1554
Hin6I GCGC 2 cut(s) 159, 1552
HinP1I GCGC 2 cut(s) 159, 1552
HincII GTYRAC 1 cut(s) 409
HindII GTYRAC 1 cut(s) 409
HindIII AAGCTT 1 cut(s) 507
HinfI GANTC 2 cut(s) 722, 1010
HpaI GTTAAC 1 cut(s) 409
HpaII CCGG 1 cut(s) 678
HphI GGTGA 6 cut(s) 226, 608, 835, 959, 1259, 1346
Hpy166II GTNNAC 3 cut(s) 409, 790, 1455
Hpy188I TCNGA 4 cut(s) 816, 868, 920, 1099
Hpy188III TCNNGA 7 cut(s) 113, 248, 372, 1214, 1325, 1384, 1534
Hpy8I GTNNAC 3 cut(s) 409, 790, 1455
Hpy99I CGWCG 1 cut(s) 1525
HpyAV CCTTC 7 cut(s) 179, 241, 322, 464, 1379, 1381, 1442
HpyCH4III ACNGT 1 cut(s) 1459
HpyCH4IV ACGT 2 cut(s) 620, 1523
HpyCH4V TGCA 9 cut(s) 10, 59, 191, 521, 773, 967, 1073, 1106, 1415
HpyF10VI GCNNNNNNNGC 6 cut(s) 91, 167, 773, 869, 969, 1070
HpyF3I CTNAG 6 cut(s) 81, 395, 1086, 1170, 1269, 1596
HpySE526I ACGT 2 cut(s) 620, 1523
HspAI GCGC 2 cut(s) 159, 1552
Ksp22I TGATCA 1 cut(s) 289
KspAI GTTAAC 1 cut(s) 409
Kzo9I GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
LguI GCTCTTC 1 cut(s) 1326
LmnI GCTCC 1 cut(s) 1443
Lsp1109I GCAGC 8 cut(s) 64, 173, 279, 763, 785, 1085, 1162, 1388
LweI GCATC 7 cut(s) 121, 179, 334, 508, 674, 877, 1306
MabI ACCWGGT 1 cut(s) 177
MaeI CTAG 3 cut(s) 839, 1065, 1310
MaeII ACGT 2 cut(s) 620, 1523
MaeIII GTNAC 9 cut(s) 104, 378, 478, 596, 745, 947, 1109, 1129, 1364
MalI GATC 8 cut(s) 22, 264, 291, 617, 668, 933, 1122, 1251
MboI GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
MboII GAAGA 6 cut(s) 212, 527, 890, 1019, 1246, 1313
MfeI CAATTG 1 cut(s) 660
MflI RGATCY 3 cut(s) 20, 666, 931
MmeI TCCRAC 1 cut(s) 1567
Mph1103I ATGCAT 2 cut(s) 12, 523
MroXI GAANNNNTTC 2 cut(s) 453, 801
MseI TTAA 7 cut(s) 138, 408, 536, 561, 1022, 1545, 1609
MslI CAYNNNNRTG 1 cut(s) 495
MspA1I CMGCKG 2 cut(s) 776, 1076
MspCI CTTAAG 2 cut(s) 535, 1544
MspI CCGG 1 cut(s) 678
MspR9I CCNGG 3 cut(s) 144, 179, 678
MunI CAATTG 1 cut(s) 660
MvaI CCWGG 2 cut(s) 144, 179
MwoI GCNNNNNNNGC 6 cut(s) 91, 167, 773, 869, 969, 1070
NciI CCSGG 1 cut(s) 678
NcoI CCATGG 1 cut(s) 460
NdeI CATATG 2 cut(s) 221, 523
NdeII GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
NlaIV GGNNCC 3 cut(s) 668, 933, 1508
NmuCI GTSAC 4 cut(s) 478, 596, 745, 947
NsiI ATGCAT 2 cut(s) 12, 523
NspI RCATGY 2 cut(s) 967, 1130
PaeI GCATGC 1 cut(s) 967
PagI TCATGA 2 cut(s) 1213, 1324
PceI AGGCCT 1 cut(s) 972
PciI ACATGT 1 cut(s) 1126
PciSI GCTCTTC 1 cut(s) 1326
PdmI GAANNNNTTC 2 cut(s) 453, 801
PfeI GAWTC 2 cut(s) 722, 1010
PflFI GACNNNGTC 1 cut(s) 1286
PkrI GCNGC 8 cut(s) 79, 163, 269, 775, 778, 1075, 1177, 1378
PscI ACATGT 1 cut(s) 1126
PsiI TTATAA 1 cut(s) 1566
Psp6I CCWGG 2 cut(s) 142, 177
PspEI GGTNACC 1 cut(s) 596
PspGI CCWGG 2 cut(s) 142, 177
PspN4I GGNNCC 3 cut(s) 668, 933, 1508
PspPI GGNCC 4 cut(s) 93, 212, 369, 1166
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 3 cut(s) 20, 666, 931
PsyI GACNNNGTC 1 cut(s) 1286
PvuII CAGCTG 2 cut(s) 776, 1076
RsaI GTAC 3 cut(s) 791, 1083, 1224
RsaNI GTAC 3 cut(s) 790, 1082, 1223
RseI CAYNNNNRTG 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 1326
SaqAI TTAA 7 cut(s) 138, 408, 536, 561, 1022, 1545, 1609
SatI GCNGC 8 cut(s) 78, 162, 268, 774, 777, 1074, 1176, 1377
Sau3AI GATC 8 cut(s) 20, 262, 289, 615, 666, 931, 1120, 1249
Sau96I GGNCC 4 cut(s) 93, 212, 369, 1166
ScrFI CCNGG 3 cut(s) 144, 179, 678
SexAI ACCWGGT 1 cut(s) 177
SfaNI GCATC 7 cut(s) 121, 179, 334, 508, 674, 877, 1306
SinI GGWCC 3 cut(s) 212, 369, 1166
SmiMI CAYNNNNRTG 1 cut(s) 495
SmlI CTYRAG 3 cut(s) 504, 535, 1544
SmoI CTYRAG 3 cut(s) 504, 535, 1544
SpeI ACTAGT 1 cut(s) 838
SphI GCATGC 1 cut(s) 967
SseBI AGGCCT 1 cut(s) 972
SspI AATATT 2 cut(s) 428, 1570
SspMI CTAG 3 cut(s) 839, 1065, 1310
StuI AGGCCT 1 cut(s) 972
StyD4I CCNGG 3 cut(s) 142, 177, 676
StyI CCWWGG 1 cut(s) 460
TaaI ACNGT 1 cut(s) 1459
TaiI ACGT 2 cut(s) 623, 1526
TaqI TCGA 5 cut(s) 342, 732, 898, 1462, 1520
TaqII GACCGA 1 cut(s) 615
TatI WGTACW 2 cut(s) 789, 1222
TfiI GAWTC 2 cut(s) 722, 1010
Tru1I TTAA 7 cut(s) 138, 408, 536, 561, 1022, 1545, 1609
Tru9I TTAA 7 cut(s) 138, 408, 536, 561, 1022, 1545, 1609
TscAI CASTG 5 cut(s) 55, 265, 497, 617, 854
TseFI GTSAC 4 cut(s) 478, 596, 745, 947
TseI GCWGC 8 cut(s) 77, 161, 267, 773, 776, 1073, 1175, 1376
Tsp45I GTSAC 4 cut(s) 478, 596, 745, 947
TspDTI ATGAA 7 cut(s) 341, 350, 462, 882, 1020, 1202, 1313
TspGWI ACGGA 1 cut(s) 1513
TspRI CASTG 5 cut(s) 55, 265, 497, 617, 854
Tth111I GACNNNGTC 1 cut(s) 1286
Vha464I CTTAAG 2 cut(s) 535, 1544
VpaK11BI GGWCC 3 cut(s) 212, 369, 1166
XapI RAATTY 4 cut(s) 125, 799, 995, 1209
XceI RCATGY 2 cut(s) 967, 1130
XmiI GTMKAC 1 cut(s) 1454
XmnI GAANNNNTTC 2 cut(s) 453, 801
XspI CTAG 3 cut(s) 839, 1065, 1310
Zsp2I ATGCAT 2 cut(s) 12, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.