Rh5DG546600

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000117
Physical Location & Seq
Forward (+)
83987842 .. 84026864
39023 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG546600.1

Sequence Viewer

Length: 1383 bp
ATGGCAATGCAAGGATCTCTGTTTCTAGGCGTAGTGCTTCTACTTGTTACAGGCTTTAGATTGACAAGCAGTGAAGCTTCTACAGCCAGTACTCCTATCTATGACACTGGTTTCCTCAACAGGACTAGTTTCCCAGCAGGTTTCATATTTGGGACAGGTTCAACGTCTTATCAGTATGAAGGTGCTGCTAAAGAAGGTGGGAGAGGACCGAGCATATGGGATACCTACACCCACGAACATCCAGAAAAGATCAAGGATGGCAGCAATGGAGATGTGGCTAGTGATGAATATCACCGCTATAAGGAAGATGTGGGGATTATGAAGAACATGGAATTGGATGCATATCGGTTCTCTATCTCATGGTCCAGATTGTTACCAAATGGAAAGCTAACTGGGGGCGTGAACAAGGAAGGAGTCAAATACTACAACAATCTCATCAATGAACTCTTACGCAATGGTCTAAAGCCATTTGTAACCCTCTTCCACTGGGATCTTCCCCAGACTTTAGAAGATGAATATGGCGGTTTCTTAAGCCCTCTCATTGTCAATCATTTTCGGGACTATGCAGAGCTTTGTTACAAGGAATTTGGTGATAGGGTAAAACACTGGATCACTTTTAATGAGCCATATAGTTATAGTGCTGGTGGTTATGCAGTGGCAATTTTGGCACCAGGACGCTGTTCTGATTGGCAGGAACTAAATTGCACCGGTGGGAATTCGGGTACTGAACCATACTTGGTGGCACACAACCAGCTCCTTGCTCATACAGCTGCAGTAACATTATACAAGCAGAAATATCAGTCATCTCAAAAGGGATTGATAGGGATTACTCTAGTGTCAAACTGGTTTGTGCCGGTTTCTGAGGCAGAGCACCATAAAAATGCTGCACTAAGAGCTCTAGATTATATGTTTGGATGGTTTATGGACCCCTTGACAAATGGTGATTATCCACACAGCATGAGATCTCTTGTTGGAAACCGATTACCCAAATTCACAAAGGAACAATCCAAGTTGCTAATTGGATCATTTGATTTTCTGGGATTGAATTACTACACAGCTAATTATGCAGCCGATGCACCTCACTATAATTCTGTCAATGCAAGCTACTTGACAGACGCTCATGCTACTCTTTCCTATGTGCTTAATGGAATTCCCATTGGACCAAAGGCTGCTTCAGATTGGCTATACGTTTATCCCAGAGGAATTCGAGATCTTTTGCTCTACACGAAGACAAAGTATAATGATCCACTCATATACGTTACTGAGAATGGTATTGATGAGTTCAATGATCCCAAATTAACACTTGAAGTATCCCTTAACGACACCCAGAGAGTTGACTACTACTATCGCCACCTCTATTACCTTCAAAGAGCAATCAAGTGA

Protein Analysis

460

Amino Acids

51.96

Weight (kDa)

6.06

Isoelectric Point (pI)

23.85

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 42 - 459 4.1e-129 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 128
AccB1I GGYRCC 1 cut(s) 667
AciI CCGC 2 cut(s) 295, 522
AclWI GGATC 6 cut(s) 22, 498, 617, 1030, 1238, 1283
AcsI RAATTY 5 cut(s) 584, 715, 989, 1149, 1203
AcuI CTGAAG 1 cut(s) 1158
AfaI GTAC 2 cut(s) 91, 724
AfiI CCNNNNNNNGG 1 cut(s) 301
AflII CTTAAG 1 cut(s) 529
AgeI ACCGGT 1 cut(s) 707
AgsI TTSAA 5 cut(s) 162, 1045, 1285, 1307, 1367
AhlI ACTAGT 1 cut(s) 125
AjnI CCWGG 1 cut(s) 670
AjuI GAANNNNNNNTTGG 4 cut(s) 317, 349, 1156, 1188
AluBI AGCT 8 cut(s) 77, 388, 571, 754, 770, 896, 1058, 1104
AluI AGCT 8 cut(s) 77, 388, 571, 754, 770, 896, 1058, 1104
Alw21I GWGCWC 2 cut(s) 873, 898
AlwI GGATC 6 cut(s) 22, 498, 617, 1030, 1238, 1283
ApeKI GCWGC 6 cut(s) 185, 261, 770, 884, 1067, 1169
ApoI RAATTY 5 cut(s) 584, 715, 989, 1149, 1203
AsiGI ACCGGT 1 cut(s) 707
AspS9I GGNCC 4 cut(s) 206, 363, 925, 1160
AsuHPI GGTGA 3 cut(s) 284, 602, 953
AvaII GGWCC 4 cut(s) 206, 363, 925, 1160
BanI GGYRCC 1 cut(s) 667
BanII GRGCYC 1 cut(s) 898
BbsI GAAGAC 1 cut(s) 1235
Bbv12I GWGCWC 2 cut(s) 873, 898
BbvI GCAGC 6 cut(s) 172, 273, 757, 871, 1079, 1156
BccI CCATC 2 cut(s) 251, 909
BciT130I CCWGG 1 cut(s) 672
BciVI GTATCC 2 cut(s) 214, 1321
BcuI ACTAGT 1 cut(s) 125
BfaI CTAG 5 cut(s) 26, 126, 279, 833, 899
BfmI CTRYAG 2 cut(s) 81, 771
BfrI CTTAAG 1 cut(s) 529
BfuAI ACCTGC 1 cut(s) 128
BfuI GTATCC 2 cut(s) 214, 1321
BglII AGATCT 2 cut(s) 962, 1210
BisI GCNGC 6 cut(s) 186, 262, 771, 885, 1068, 1170
BlsI GCNGC 6 cut(s) 187, 263, 772, 886, 1069, 1171
BmcAI AGTACT 1 cut(s) 91
Bme1390I CCNGG 1 cut(s) 672
Bme18I GGWCC 4 cut(s) 206, 363, 925, 1160
BmgT120I GGNCC 4 cut(s) 206, 363, 925, 1160
BmiI GGNNCC 2 cut(s) 669, 927
BmrFI CCNGG 1 cut(s) 672
BmrI ACTGGG 2 cut(s) 402, 496
BmsI GCATC 2 cut(s) 328, 1063
BmuI ACTGGG 2 cut(s) 402, 496
BpiI GAAGAC 1 cut(s) 1235
BsaBI GATNNNNATC 2 cut(s) 288, 342
BsaWI WCCGGW 1 cut(s) 707
Bsc4I CCNNNNNNNGG 1 cut(s) 301
Bse118I RCCGGY 2 cut(s) 707, 853
Bse1I ACTGG 6 cut(s) 87, 112, 397, 491, 611, 848
Bse3DI GCAATG 3 cut(s) 12, 271, 460
Bse8I GATNNNNATC 2 cut(s) 288, 342
BseBI CCWGG 1 cut(s) 672
BseGI GGATG 4 cut(s) 238, 262, 343, 920
BseJI GATNNNNATC 2 cut(s) 288, 342
BseLI CCNNNNNNNGG 1 cut(s) 301
BseMI GCAATG 3 cut(s) 12, 271, 460
BseMII CTCAG 2 cut(s) 852, 1254
BseNI ACTGG 6 cut(s) 87, 112, 397, 491, 611, 848
BseXI GCAGC 6 cut(s) 172, 273, 757, 871, 1079, 1156
BseYI CCCAGC 1 cut(s) 133
BsgI GTGCAG 1 cut(s) 870
BshNI GGYRCC 1 cut(s) 667
BshTI ACCGGT 1 cut(s) 707
BsiHKAI GWGCWC 2 cut(s) 873, 898
BsiSI CCGG 2 cut(s) 708, 854
BslFI GGGAC 2 cut(s) 166, 572
BslI CCNNNNNNNGG 1 cut(s) 301
BsmFI GGGAC 2 cut(s) 166, 572
Bsp1286I GDGCHC 2 cut(s) 873, 898
Bsp143I GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
BspACI CCGC 2 cut(s) 295, 522
BspCNI CTCAG 2 cut(s) 853, 1255
BspLI GGNNCC 2 cut(s) 669, 927
BspMAI CTGCAG 1 cut(s) 775
BspMI ACCTGC 1 cut(s) 128
BspPI GGATC 6 cut(s) 22, 498, 617, 1030, 1238, 1283
BspT107I GGYRCC 1 cut(s) 667
BspTI CTTAAG 1 cut(s) 529
BsrDI GCAATG 3 cut(s) 12, 271, 460
BsrFI RCCGGY 2 cut(s) 707, 853
BsrI ACTGG 6 cut(s) 87, 112, 397, 491, 611, 848
BssAI RCCGGY 2 cut(s) 707, 853
BssMI GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
Bst2UI CCWGG 1 cut(s) 672
Bst6I CTCTTC 1 cut(s) 485
BstAFI CTTAAG 1 cut(s) 529
BstAPI GCANNNNNTGC 1 cut(s) 1073
BstC8I GCNNGC 1 cut(s) 1102
BstDEI CTNAG 3 cut(s) 861, 890, 1263
BstF5I GGATG 4 cut(s) 238, 262, 343, 920
BstKTI GATC 9 cut(s) 17, 252, 493, 612, 965, 1025, 1213, 1246, 1291
BstMBI GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
BstMWI GCNNNNNNNGC 6 cut(s) 83, 665, 767, 893, 1064, 1073
BstNI CCWGG 1 cut(s) 672
BstSCI CCNGG 1 cut(s) 670
BstSFI CTRYAG 2 cut(s) 81, 771
BstV1I GCAGC 6 cut(s) 172, 273, 757, 871, 1079, 1156
BstV2I GAAGAC 1 cut(s) 1235
BstX2I RGATCY 4 cut(s) 14, 490, 962, 1210
BstYI RGATCY 4 cut(s) 14, 490, 962, 1210
BsuI GTATCC 2 cut(s) 214, 1321
BtsCI GGATG 4 cut(s) 238, 262, 343, 920
BtsI GCAGTG 2 cut(s) 76, 660
BtsIMutI CAGTG 5 cut(s) 76, 105, 484, 604, 660
BveI ACCTGC 1 cut(s) 128
Cac8I GCNNGC 1 cut(s) 1102
Cfr10I RCCGGY 2 cut(s) 707, 853
Cfr13I GGNCC 4 cut(s) 206, 363, 925, 1160
CseI GACGC 2 cut(s) 684, 1124
Csp6I GTAC 2 cut(s) 90, 723
CspAI ACCGGT 1 cut(s) 707
CviAII CATG 4 cut(s) 328, 360, 958, 1121
CviQI GTAC 2 cut(s) 90, 723
DdeI CTNAG 3 cut(s) 861, 890, 1263
DpnI GATC 9 cut(s) 16, 251, 492, 611, 964, 1024, 1212, 1245, 1290
DpnII GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
Eam1104I CTCTTC 1 cut(s) 485
EarI CTCTTC 1 cut(s) 485
Ecl136II GAGCTC 1 cut(s) 896
Eco24I GRGCYC 1 cut(s) 898
Eco47I GGWCC 4 cut(s) 206, 363, 925, 1160
Eco53kI GAGCTC 1 cut(s) 896
Eco57I CTGAAG 1 cut(s) 1158
EcoICRI GAGCTC 1 cut(s) 896
EcoRI GAATTC 3 cut(s) 715, 1149, 1203
EcoRII CCWGG 1 cut(s) 670
EcoT22I ATGCAT 1 cut(s) 343
EcoT38I GRGCYC 1 cut(s) 898
FaeI CATG 4 cut(s) 331, 363, 961, 1124
FalI AAGNNNNNCTT 2 cut(s) 1299, 1331
FaqI GGGAC 2 cut(s) 166, 572
FatI CATG 4 cut(s) 327, 359, 957, 1120
FauNDI CATATG 1 cut(s) 215
Fnu4HI GCNGC 6 cut(s) 186, 262, 771, 885, 1068, 1170
FokI GGATG 4 cut(s) 225, 269, 350, 927
FriOI GRGCYC 1 cut(s) 898
Fsp4HI GCNGC 6 cut(s) 186, 262, 771, 885, 1068, 1170
FspBI CTAG 5 cut(s) 26, 126, 279, 833, 899
GluI GCNGC 6 cut(s) 186, 262, 771, 885, 1068, 1170
GsaI CCCAGC 1 cut(s) 137
HapII CCGG 2 cut(s) 708, 854
HgaI GACGC 2 cut(s) 684, 1124
Hin1II CATG 4 cut(s) 331, 363, 961, 1124
HincII GTYRAC 1 cut(s) 1336
HindII GTYRAC 1 cut(s) 1336
HindIII AAGCTT 1 cut(s) 75
HinfI GANTC 1 cut(s) 414
HpaII CCGG 2 cut(s) 708, 854
HphI GGTGA 3 cut(s) 284, 602, 953
Hpy166II GTNNAC 2 cut(s) 403, 1336
Hpy188I TCNGA 3 cut(s) 685, 862, 1177
Hpy188III TCNNGA 5 cut(s) 242, 366, 557, 899, 1208
Hpy8I GTNNAC 2 cut(s) 403, 1336
HpyAV CCTTC 4 cut(s) 173, 188, 404, 1373
HpyCH4IV ACGT 3 cut(s) 164, 1188, 1257
HpyF10VI GCNNNNNNNGC 6 cut(s) 83, 665, 767, 893, 1064, 1073
HpyF3I CTNAG 3 cut(s) 861, 890, 1263
HpySE526I ACGT 3 cut(s) 164, 1188, 1257
Hsp92II CATG 4 cut(s) 331, 363, 961, 1124
Kzo9I GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
LmnI GCTCC 1 cut(s) 759
Lsp1109I GCAGC 6 cut(s) 172, 273, 757, 871, 1079, 1156
LweI GCATC 2 cut(s) 328, 1063
MaeI CTAG 5 cut(s) 26, 126, 279, 833, 899
MaeII ACGT 3 cut(s) 164, 1188, 1257
MaeIII GTNAC 6 cut(s) 46, 372, 472, 575, 775, 1258
MalI GATC 9 cut(s) 16, 251, 492, 611, 964, 1024, 1212, 1245, 1290
MboI GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
MboII GAAGA 6 cut(s) 317, 334, 472, 485, 521, 1240
MflI RGATCY 4 cut(s) 14, 490, 962, 1210
MhlI GDGCHC 2 cut(s) 873, 898
MlyI GAGTC 1 cut(s) 423
MmeI TCCRAC 1 cut(s) 952
MnlI CCTC 8 cut(s) 125, 197, 488, 546, 856, 1089, 1193, 1364
Mph1103I ATGCAT 1 cut(s) 343
MseI TTAA 5 cut(s) 530, 618, 1143, 1298, 1317
MslI CAYNNNNRTG 1 cut(s) 879
MspA1I CMGCKG 1 cut(s) 770
MspCI CTTAAG 1 cut(s) 529
MspI CCGG 2 cut(s) 708, 854
MspR9I CCNGG 1 cut(s) 672
MvaI CCWGG 1 cut(s) 672
MwoI GCNNNNNNNGC 6 cut(s) 83, 665, 767, 893, 1064, 1073
NdeI CATATG 1 cut(s) 215
NdeII GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
NlaIII CATG 4 cut(s) 331, 363, 961, 1124
NlaIV GGNNCC 2 cut(s) 669, 927
NsiI ATGCAT 1 cut(s) 343
PinAI ACCGGT 1 cut(s) 707
PkrI GCNGC 6 cut(s) 187, 263, 772, 886, 1069, 1171
PleI GAGTC 1 cut(s) 422
PpsI GAGTC 1 cut(s) 422
Psp124BI GAGCTC 1 cut(s) 898
Psp6I CCWGG 1 cut(s) 670
PspFI CCCAGC 1 cut(s) 133
PspGI CCWGG 1 cut(s) 670
PspN4I GGNNCC 2 cut(s) 669, 927
PspPI GGNCC 4 cut(s) 206, 363, 925, 1160
PstI CTGCAG 1 cut(s) 775
PsuI RGATCY 4 cut(s) 14, 490, 962, 1210
PvuII CAGCTG 1 cut(s) 770
RsaI GTAC 2 cut(s) 91, 724
RsaNI GTAC 2 cut(s) 90, 723
RseI CAYNNNNRTG 1 cut(s) 879
SacI GAGCTC 1 cut(s) 898
SaqAI TTAA 5 cut(s) 530, 618, 1143, 1298, 1317
SatI GCNGC 6 cut(s) 186, 262, 771, 885, 1068, 1170
Sau3AI GATC 9 cut(s) 14, 249, 490, 609, 962, 1022, 1210, 1243, 1288
Sau96I GGNCC 4 cut(s) 206, 363, 925, 1160
ScaI AGTACT 1 cut(s) 91
SchI GAGTC 1 cut(s) 423
ScrFI CCNGG 1 cut(s) 672
SduI GDGCHC 2 cut(s) 873, 898
SfaNI GCATC 2 cut(s) 328, 1063
SfcI CTRYAG 2 cut(s) 81, 771
SgrAI CRCCGGYG 1 cut(s) 707
SinI GGWCC 4 cut(s) 206, 363, 925, 1160
SmiMI CAYNNNNRTG 1 cut(s) 879
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
SpeI ACTAGT 1 cut(s) 125
SsiI CCGC 2 cut(s) 295, 522
SspMI CTAG 5 cut(s) 26, 126, 279, 833, 899
SstI GAGCTC 1 cut(s) 898
StyD4I CCNGG 1 cut(s) 670
TaiI ACGT 3 cut(s) 167, 1191, 1260
TaqI TCGA 1 cut(s) 1207
TaqII GACCGA 1 cut(s) 223
TatI WGTACW 1 cut(s) 89
Tru1I TTAA 5 cut(s) 530, 618, 1143, 1298, 1317
Tru9I TTAA 5 cut(s) 530, 618, 1143, 1298, 1317
TscAI CASTG 5 cut(s) 76, 112, 491, 611, 660
TseI GCWGC 6 cut(s) 185, 261, 770, 884, 1067, 1169
TspDTI ATGAA 6 cut(s) 133, 192, 300, 335, 456, 528
TspRI CASTG 5 cut(s) 76, 112, 491, 611, 660
Vha464I CTTAAG 1 cut(s) 529
VpaK11BI GGWCC 4 cut(s) 206, 363, 925, 1160
XapI RAATTY 5 cut(s) 584, 715, 989, 1149, 1203
XbaI TCTAGA 1 cut(s) 898
XspI CTAG 5 cut(s) 26, 126, 279, 833, 899
ZrmI AGTACT 1 cut(s) 91
Zsp2I ATGCAT 1 cut(s) 343
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.