MD09G1243300.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr09
OG0000117
Physical Location & Seq
Reverse (-)
31044870 .. 31051345
6476 bp
Loading structure...
UTR
Exon/CDS
Intron
MD09G1243300.v1.1.491

Sequence Viewer

Length: 1578 bp
ATGCATCTAAATCACCAAAACTTACAAGTTATGTCACTGCAATTAGGGTCTCCTCTGTCATGTGTGCTGCTGCTTCTAGTTGGCGTTGCATTGACAAGTAGTAAAGCTGCGATTACGCCCAGTCGTTATGACACTGCTTTCCTCAACAGGAGCAGTTTTCCAGCAGGGTTTATATTTGGCACTGCTTCAGCATCTTACCAGTATGAAGGTGCTGCTAGAGAAGATGGTAGAGGACCAAGCATATGGGACACCTACACCCACAAATATCCAGAGAAGATCACAGATGGAAGCAATGGAGATGTGGCTAATGATGAATATCACCGTTATAAGGAAGATGTGGGAATTATGAAGAATATGGGGTTGGATGCTTATAGATTCTCTATTTCATGGTCCAGATTGTTACCGAATGGAAAGCTAAGTGGGGGCATTAACAAGGAAGGAATCAAATACTACAACAATCTCATCAATGAACTTTTACGCCATGGTGTAAAGCCATTTGTGACACTTTTTCATTTTGATCTTCCTCAAGCTTTAGAAGATGAATACGGCGGTTTCTTAAGCCCTAAAATTGTACAACATTTTCGAGACTATGCAAAACTTTGTTACAATGAATTTGGCGACCGCGTAAAACATTGGATCACGTTGAATGAGCCATGGACCTATAGCAACGGTGGTTATGCTAGCGGATTTTTAGCACCAGGACGATGTTCTGCTTGGCAGCAACTAAACTGCACTGGTGGAAATTCGGCTACTGAACCATACTTGGTGGCACACCACCAACTCCTTGCTCATGCAGCTGCTGTAAAAGTATACAAGGATAAATATCAGGCATCTCAAAAAGGCTTGATATCAATAACACTGGCGTCACTTTGGTTTGTTCCGGTTTCTGATGCAAAGCACCAAAAAAACGCTGCGTTGAGATCTTTGGATTTTATGTTTGGATGGTTTATGGAACCATTGACGAGTGGCGACTATCCACACAGCATGCGGTCTCTTGTAGGAAGCCGATTACCAAAGTTTACGAAAGAACAATCCAAGTTGCTAAAGGGGTCATTTGATTTTCTTGGATTAAATTACTATACCGCTTACTATGCAAGTTATGCACCCTCAAACAATTCTGTAAATGCAAGCTATTTAACAGATGCTCGCTCTAATCAATCTCCTGAGAAAAATGGAGTCCCCATCGGTCCAAAGGCTGCTTCAGATTGGCTATACGTTTATCCAAGAGGAATTCAAGACCTTTTACTCTACACGAAGGAGAAGTATCATGATCCACTCATTTACATTACTGAGAATGGTGTTGATGAGTTCAATGATCCCAAATTATCACTTATAGAAGCCCTTAATGACACCCAACGAGTTTATTACTACTATCACCACATATATTACCTTCACAGAGCAATCAAGGATGGTGTCAATGTGAAGGGATACTTTGCATGGTCGTTATTGGATAATTTTGAATGGAGTTCGGGATACACTGTTCGGTTTGGGATCAATTATGTAGATTACAACGACAACCGAAAAAGGTACCCCAAACTCTCAGCACACTGGTTCAAAAGTTTCCTCAAGAAGTACTGA

Protein Analysis

526

Amino Acids

59.8

Weight (kDa)

8.8

Isoelectric Point (pI)

31.81

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 51 - 524 1.9e-161 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 327
Acc65I GGTACC 1 cut(s) 1527
AccB1I GGYRCC 1 cut(s) 1527
AccI GTMKAC 1 cut(s) 810
AccII CGCG 1 cut(s) 624
AciI CCGC 5 cut(s) 549, 622, 684, 988, 1083
AclWI GGATC 4 cut(s) 644, 1265, 1310, 1499
AcsI RAATTY 3 cut(s) 611, 742, 1230
AcuI CTGAAG 2 cut(s) 171, 1185
AcyI GRCGYC 1 cut(s) 863
AfaI GTAC 3 cut(s) 573, 1529, 1574
AfiI CCNNNNNNNGG 1 cut(s) 328
AflII CTTAAG 1 cut(s) 556
AgsI TTSAA 5 cut(s) 646, 1235, 1312, 1460, 1555
AjnI CCWGG 1 cut(s) 697
AjuI GAANNNNNNNTTGG 4 cut(s) 344, 376, 1183, 1215
AloI GAACNNNNNNTCC 2 cut(s) 1464, 1496
AluBI AGCT 5 cut(s) 107, 415, 530, 797, 1131
AluI AGCT 5 cut(s) 107, 415, 530, 797, 1131
Alw26I GTCTC 3 cut(s) 54, 579, 996
AlwI GGATC 4 cut(s) 644, 1265, 1310, 1499
AlwNI CAGNNNCTG 1 cut(s) 800
ApeKI GCWGC 9 cut(s) 67, 70, 107, 212, 718, 794, 797, 911, 1196
ApoI RAATTY 3 cut(s) 611, 742, 1230
Asp718I GGTACC 1 cut(s) 1527
AspS9I GGNCC 4 cut(s) 233, 390, 657, 1187
AsuHPI GGTGA 3 cut(s) 5, 311, 1367
AsuNHI GCTAGC 1 cut(s) 680
AvaII GGWCC 4 cut(s) 233, 390, 657, 1187
BanI GGYRCC 1 cut(s) 1527
BbvI GCAGC 9 cut(s) 54, 57, 94, 199, 730, 784, 806, 898, 1183
BccI CCATC 5 cut(s) 218, 278, 936, 1190, 1403
BceAI ACGGC 1 cut(s) 562
BciT130I CCWGG 1 cut(s) 699
BciVI GTATCC 2 cut(s) 1421, 1466
BcoDI GTCTC 3 cut(s) 54, 579, 996
BfaI CTAG 3 cut(s) 77, 216, 681
BfmI CTRYAG 1 cut(s) 661
BfrI CTTAAG 1 cut(s) 556
BfuI GTATCC 2 cut(s) 1421, 1466
BglII AGATCT 1 cut(s) 920
BisI GCNGC 9 cut(s) 68, 71, 108, 213, 719, 795, 798, 912, 1197
BlsI GCNGC 9 cut(s) 69, 72, 109, 214, 720, 796, 799, 913, 1198
BmcAI AGTACT 1 cut(s) 1574
Bme1390I CCNGG 1 cut(s) 699
Bme18I GGWCC 4 cut(s) 233, 390, 657, 1187
BmgT120I GGNCC 4 cut(s) 233, 390, 657, 1187
BmiI GGNNCC 2 cut(s) 954, 1529
BmrFI CCNGG 1 cut(s) 699
BmrI ACTGGG 1 cut(s) 114
BmsI GCATC 6 cut(s) 13, 200, 355, 839, 880, 1132
BmtI GCTAGC 1 cut(s) 684
BmuI ACTGGG 1 cut(s) 114
BpuEI CTTGAG 2 cut(s) 510, 1550
BsaBI GATNNNNATC 2 cut(s) 315, 822
BsaHI GRCGYC 1 cut(s) 863
BsaI GGTCTC 2 cut(s) 54, 996
BsaJI CCNNGG 2 cut(s) 481, 653
BsaWI WCCGGW 1 cut(s) 880
Bsc4I CCNNNNNNNGG 1 cut(s) 328
Bse1I ACTGG 5 cut(s) 120, 199, 739, 864, 1553
Bse3DI GCAATG 1 cut(s) 298
Bse8I GATNNNNATC 2 cut(s) 315, 822
BseBI CCWGG 1 cut(s) 699
BseDI CCNNGG 2 cut(s) 481, 653
BseGI GGATG 3 cut(s) 370, 947, 1414
BseJI GATNNNNATC 2 cut(s) 315, 822
BseLI CCNNNNNNNGG 1 cut(s) 328
BseMI GCAATG 1 cut(s) 298
BseMII CTCAG 3 cut(s) 1155, 1281, 1554
BseNI ACTGG 5 cut(s) 120, 199, 739, 864, 1553
BseRI GAGGAG 1 cut(s) 42
BseXI GCAGC 9 cut(s) 54, 57, 94, 199, 730, 784, 806, 898, 1183
BsgI GTGCAG 1 cut(s) 715
Bsh1236I CGCG 1 cut(s) 624
Bsh1285I CGRYCG 1 cut(s) 622
BshNI GGYRCC 1 cut(s) 1527
BsiEI CGRYCG 1 cut(s) 622
BsiSI CCGG 1 cut(s) 881
BslFI GGGAC 2 cut(s) 260, 1163
BslI CCNNNNNNNGG 1 cut(s) 328
BsmAI GTCTC 3 cut(s) 54, 579, 996
BsmFI GGGAC 2 cut(s) 260, 1163
Bso31I GGTCTC 2 cut(s) 54, 996
Bsp1407I TGTACA 1 cut(s) 571
Bsp143I GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
Bsp19I CCATGG 2 cut(s) 481, 653
BspACI CCGC 5 cut(s) 549, 622, 684, 988, 1083
BspCNI CTCAG 3 cut(s) 1156, 1282, 1553
BspFNI CGCG 1 cut(s) 624
BspHI TCATGA 1 cut(s) 1267
BspLI GGNNCC 2 cut(s) 954, 1529
BspOI GCTAGC 1 cut(s) 684
BspPI GGATC 4 cut(s) 644, 1265, 1310, 1499
BspT107I GGYRCC 1 cut(s) 1527
BspTI CTTAAG 1 cut(s) 556
BspTNI GGTCTC 2 cut(s) 54, 996
BsrDI GCAATG 1 cut(s) 298
BsrGI TGTACA 1 cut(s) 571
BsrI ACTGG 5 cut(s) 120, 199, 739, 864, 1553
BssECI CCNNGG 2 cut(s) 481, 653
BssMI GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
BssNAI GTATAC 1 cut(s) 811
BssNI GRCGYC 1 cut(s) 863
BssT1I CCWWGG 2 cut(s) 481, 653
Bst1107I GTATAC 1 cut(s) 811
Bst2UI CCWGG 1 cut(s) 699
Bst4CI ACNGT 3 cut(s) 323, 671, 1480
BstACI GRCGYC 1 cut(s) 863
BstAFI CTTAAG 1 cut(s) 556
BstAPI GCANNNNNTGC 1 cut(s) 1100
BstAUI TGTACA 1 cut(s) 571
BstC8I GCNNGC 4 cut(s) 682, 986, 1129, 1147
BstDEI CTNAG 4 cut(s) 416, 1164, 1290, 1540
BstDSI CCRYGG 2 cut(s) 481, 653
BstF5I GGATG 3 cut(s) 370, 947, 1414
BstFNI CGCG 1 cut(s) 624
BstKTI GATC 7 cut(s) 279, 520, 639, 923, 1273, 1318, 1494
BstMAI GTCTC 3 cut(s) 54, 579, 996
BstMBI GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
BstMCI CGRYCG 1 cut(s) 622
BstMWI GCNNNNNNNGC 3 cut(s) 794, 1091, 1100
BstNI CCWGG 1 cut(s) 699
BstNSI RCATGY 1 cut(s) 988
BstSCI CCNGG 1 cut(s) 697
BstSFI CTRYAG 1 cut(s) 661
BstUI CGCG 1 cut(s) 624
BstV1I GCAGC 9 cut(s) 54, 57, 94, 199, 730, 784, 806, 898, 1183
BstX2I RGATCY 1 cut(s) 920
BstXI CCANNNNNNTGG 1 cut(s) 243
BstYI RGATCY 1 cut(s) 920
BstZ17I GTATAC 1 cut(s) 811
BsuI GTATCC 2 cut(s) 1421, 1466
BtgI CCRYGG 2 cut(s) 481, 653
BtsCI GGATG 3 cut(s) 370, 947, 1414
BtsI GCAGTG 3 cut(s) 35, 132, 180
BtsIMutI CAGTG 7 cut(s) 35, 132, 180, 732, 857, 1476, 1546
Cac8I GCNNGC 4 cut(s) 682, 986, 1129, 1147
CaiI CAGNNNCTG 1 cut(s) 800
CciI TCATGA 1 cut(s) 1267
Cfr13I GGNCC 4 cut(s) 233, 390, 657, 1187
CseI GACGC 1 cut(s) 852
Csp6I GTAC 3 cut(s) 572, 1528, 1573
CviAII CATG 8 cut(s) 60, 387, 482, 654, 791, 985, 1268, 1437
CviQI GTAC 3 cut(s) 572, 1528, 1573
DdeI CTNAG 4 cut(s) 416, 1164, 1290, 1540
DpnI GATC 7 cut(s) 278, 519, 638, 922, 1272, 1317, 1493
DpnII GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
Eco130I CCWWGG 2 cut(s) 481, 653
Eco31I GGTCTC 2 cut(s) 54, 996
Eco32I GATATC 1 cut(s) 849
Eco47I GGWCC 4 cut(s) 233, 390, 657, 1187
Eco57I CTGAAG 2 cut(s) 171, 1185
EcoRI GAATTC 1 cut(s) 1230
EcoRII CCWGG 1 cut(s) 697
EcoRV GATATC 1 cut(s) 849
EcoT14I CCWWGG 2 cut(s) 481, 653
EcoT22I ATGCAT 1 cut(s) 6
ErhI CCWWGG 2 cut(s) 481, 653
FaeI CATG 8 cut(s) 63, 390, 485, 657, 794, 988, 1271, 1440
FalI AAGNNNNNCTT 2 cut(s) 1415, 1447
FaqI GGGAC 2 cut(s) 260, 1163
FatI CATG 8 cut(s) 59, 386, 481, 653, 790, 984, 1267, 1436
FauNDI CATATG 1 cut(s) 242
FblI GTMKAC 1 cut(s) 810
Fnu4HI GCNGC 9 cut(s) 68, 71, 108, 213, 719, 795, 798, 912, 1197
FokI GGATG 3 cut(s) 377, 954, 1421
Fsp4HI GCNGC 9 cut(s) 68, 71, 108, 213, 719, 795, 798, 912, 1197
FspBI CTAG 3 cut(s) 77, 216, 681
GluI GCNGC 9 cut(s) 68, 71, 108, 213, 719, 795, 798, 912, 1197
HapII CCGG 1 cut(s) 881
HgaI GACGC 1 cut(s) 852
Hin1I GRCGYC 1 cut(s) 863
Hin1II CATG 8 cut(s) 63, 390, 485, 657, 794, 988, 1271, 1440
HindIII AAGCTT 1 cut(s) 528
HinfI GANTC 3 cut(s) 375, 441, 1176
HpaII CCGG 1 cut(s) 881
HphI GGTGA 3 cut(s) 5, 311, 1367
Hpy166II GTNNAC 2 cut(s) 811, 1020
Hpy188I TCNGA 2 cut(s) 889, 1204
Hpy188III TCNNGA 8 cut(s) 269, 393, 584, 1163, 1235, 1268, 1470, 1567
Hpy8I GTNNAC 2 cut(s) 811, 1020
HpyAV CCTTC 5 cut(s) 200, 431, 1249, 1400, 1417
HpyCH4III ACNGT 3 cut(s) 323, 671, 1480
HpyCH4IV ACGT 2 cut(s) 641, 1215
HpyF10VI GCNNNNNNNGC 3 cut(s) 794, 1091, 1100
HpyF3I CTNAG 4 cut(s) 416, 1164, 1290, 1540
HpySE526I ACGT 2 cut(s) 641, 1215
Hsp92I GRCGYC 1 cut(s) 863
Hsp92II CATG 8 cut(s) 63, 390, 485, 657, 794, 988, 1271, 1440
KpnI GGTACC 1 cut(s) 1531
Kzo9I GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
LmnI GCTCC 1 cut(s) 150
Lsp1109I GCAGC 9 cut(s) 54, 57, 94, 199, 730, 784, 806, 898, 1183
LweI GCATC 6 cut(s) 13, 200, 355, 839, 880, 1132
MaeI CTAG 3 cut(s) 77, 216, 681
MaeII ACGT 2 cut(s) 641, 1215
MaeIII GTNAC 5 cut(s) 33, 399, 499, 602, 864
MalI GATC 7 cut(s) 278, 519, 638, 922, 1272, 1317, 1493
MboI GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
MboII GAAGA 6 cut(s) 233, 286, 344, 361, 512, 548
MflI RGATCY 1 cut(s) 920
MlyI GAGTC 1 cut(s) 1185
MmeI TCCRAC 1 cut(s) 342
MnlI CCTC 7 cut(s) 63, 152, 224, 534, 1117, 1220, 1574
Mph1103I ATGCAT 1 cut(s) 6
MseI TTAA 5 cut(s) 429, 557, 1070, 1136, 1344
MspA1I CMGCKG 1 cut(s) 797
MspCI CTTAAG 1 cut(s) 556
MspI CCGG 1 cut(s) 881
MspR9I CCNGG 1 cut(s) 699
MvaI CCWGG 1 cut(s) 699
MvnI CGCG 1 cut(s) 624
MwoI GCNNNNNNNGC 3 cut(s) 794, 1091, 1100
NcoI CCATGG 2 cut(s) 481, 653
NdeI CATATG 1 cut(s) 242
NdeII GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
NheI GCTAGC 1 cut(s) 680
NlaIII CATG 8 cut(s) 63, 390, 485, 657, 794, 988, 1271, 1440
NlaIV GGNNCC 2 cut(s) 954, 1529
NmuCI GTSAC 3 cut(s) 33, 499, 864
NsiI ATGCAT 1 cut(s) 6
NspI RCATGY 1 cut(s) 988
PaeI GCATGC 1 cut(s) 988
PagI TCATGA 1 cut(s) 1267
PfeI GAWTC 2 cut(s) 375, 441
PkrI GCNGC 9 cut(s) 69, 72, 109, 214, 720, 796, 799, 913, 1198
PleI GAGTC 1 cut(s) 1184
PpsI GAGTC 1 cut(s) 1184
PsiI TTATAA 1 cut(s) 327
Psp6I CCWGG 1 cut(s) 697
PspGI CCWGG 1 cut(s) 697
PspN4I GGNNCC 2 cut(s) 954, 1529
PspPI GGNCC 4 cut(s) 233, 390, 657, 1187
PstNI CAGNNNCTG 1 cut(s) 800
PsuI RGATCY 1 cut(s) 920
PvuII CAGCTG 1 cut(s) 797
RsaI GTAC 3 cut(s) 573, 1529, 1574
RsaNI GTAC 3 cut(s) 572, 1528, 1573
SaqAI TTAA 5 cut(s) 429, 557, 1070, 1136, 1344
SatI GCNGC 9 cut(s) 68, 71, 108, 213, 719, 795, 798, 912, 1197
Sau3AI GATC 7 cut(s) 276, 517, 636, 920, 1270, 1315, 1491
Sau96I GGNCC 4 cut(s) 233, 390, 657, 1187
ScaI AGTACT 1 cut(s) 1574
SchI GAGTC 1 cut(s) 1185
ScrFI CCNGG 1 cut(s) 699
SfaNI GCATC 6 cut(s) 13, 200, 355, 839, 880, 1132
SfcI CTRYAG 1 cut(s) 661
SinI GGWCC 4 cut(s) 233, 390, 657, 1187
SmlI CTYRAG 3 cut(s) 525, 556, 1565
SmoI CTYRAG 3 cut(s) 525, 556, 1565
SphI GCATGC 1 cut(s) 988
SsiI CCGC 5 cut(s) 549, 622, 684, 988, 1083
SspMI CTAG 3 cut(s) 77, 216, 681
StyD4I CCNGG 1 cut(s) 697
StyI CCWWGG 2 cut(s) 481, 653
TaaI ACNGT 3 cut(s) 323, 671, 1480
TaiI ACGT 2 cut(s) 644, 1218
TaqI TCGA 1 cut(s) 583
TaqII GACCGA 1 cut(s) 1175
TatI WGTACW 2 cut(s) 571, 1572
TfiI GAWTC 2 cut(s) 375, 441
Tru1I TTAA 5 cut(s) 429, 557, 1070, 1136, 1344
Tru9I TTAA 5 cut(s) 429, 557, 1070, 1136, 1344
TscAI CASTG 7 cut(s) 42, 139, 187, 739, 864, 1483, 1553
TseFI GTSAC 3 cut(s) 33, 499, 864
TseI GCWGC 9 cut(s) 67, 70, 107, 212, 718, 794, 797, 911, 1196
Tsp45I GTSAC 3 cut(s) 33, 499, 864
TspDTI ATGAA 8 cut(s) 219, 327, 362, 375, 483, 500, 555, 624
TspRI CASTG 7 cut(s) 42, 139, 187, 739, 864, 1483, 1553
Vha464I CTTAAG 1 cut(s) 556
VpaK11BI GGWCC 4 cut(s) 233, 390, 657, 1187
XapI RAATTY 3 cut(s) 611, 742, 1230
XceI RCATGY 1 cut(s) 988
XmiI GTMKAC 1 cut(s) 810
XspI CTAG 3 cut(s) 77, 216, 681
ZrmI AGTACT 1 cut(s) 1574
Zsp2I ATGCAT 1 cut(s) 6
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.