Rh5DG547000

beta-glucosidase activity

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0000117
Physical Location & Seq
Forward (+)
84140180 .. 84145191
5012 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG547000.1

Sequence Viewer

Length: 1188 bp
ATGCAGATTTCAAATTGCTCTGTCGCAGATAACTTTATGGCTCTCCGGTTAGGCATCCGTACCCTTATCTTTGGCCTCCTACTTGTTCTTAGCAGTACAGGGACAAGTAGTCATAGTAGCAGAAAAGCAGCCCCCCAAAAGAAGTCACGTAACTCTACTTCCTTCAGCCGCAGCACATTTCCGGCAGGTTTCATATTCGGGACAGCTTCCGCGGCTTACCAGTTCGAAGGGGCTGCAAAAGAAGGTGGTAGAGGACCGTGTGTATGGGACGCCTTTACTCACAAGTATCCAGAGAAGATCAAGGACCACAGCAGTGGAGATGTGGCTAATGATCAATATCATCGGTACAAGGAAGATGTGAATATGTTGAAGGACATGGGTTGGGATGCTTACAGATTCTCAATCTCATGGTCTAGACTGTTACCAAATGGAAAGCTGAGTGGAGGTGTGAACCAGGAAGGAATCAAATATTACAAGAATCTCATCAACGAGCTTCTAGCAAATGGTATAAGCCCCTACGTCACACTCTTTCATTGGGATACGCCCCAAGCTTTAGAAGAGGAATACGGTGGTTTCCTAAGCCCTCAAATTGTGAATCATTTTCAGGACTTTGCAGAGCTCTGCTATAAAGAATTTGGTGACAAAGTCAAGCATTGGACTACACTAAATGAGCCGCATACATACAGCGTCAATGGTTATGCAACCGGGGGAATGGCACCAGGCAGATGTTCTGCTTGGAAAAATCCTAATTGCACCGGTGGCGATTCAGGGACTGAGCCATATTTGGTGTCACATCACCTACTTCTAGCTCATGCCGCTGCTGTGAAGGTGTACCGAGACAAATTTCAGGCAAAACAGAAGGGTTTGATAGGGATAACGGTAAACACAGGATGGTTTGTGCCATATTCCGACTCAAAGCGTGACCAAGATGCTGCACAACGAGCAATGGATTTCAACCTCGGATGGTTTATGGATCCCGTAACACATGGTGACTATCCGCACGTCATGAGATCTCTTGTTGGAAATCGATTACCCAAGTTCACTAAAGAGCAATCACATATGCTAAAAGGATCATTTGATTTTGTTGGGTTGAACTACTACTCCGGTGGTTATGCAGCGGATTTAGCTAAACCTGATCCTAAGCAACCAAGCTACACGACAGACTCCCTCGTTAATACTACATGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

395

Amino Acids

43.95

Weight (kDa)

8.54

Isoelectric Point (pI)

28.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 57 - 390 2.8e-116 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 1 cut(s) 176
AccB1I GGYRCC 1 cut(s) 715
AccII CGCG 1 cut(s) 212
AciI CCGC 7 cut(s) 169, 210, 212, 674, 816, 998, 1118
AclWI GGATC 4 cut(s) 968, 981, 1078, 1130
AcsI RAATTY 2 cut(s) 632, 842
AcuI CTGAAG 1 cut(s) 148
AcyI GRCGYC 1 cut(s) 270
AdeI CACNNNGTG 1 cut(s) 989
AfaI GTAC 4 cut(s) 61, 97, 347, 833
AflIII ACRYGT 1 cut(s) 1181
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 4 cut(s) 12, 370, 955, 1093
AhdI GACNNNNNGTC 1 cut(s) 108
AjiI CACGTC 1 cut(s) 1003
AjnI CCWGG 2 cut(s) 453, 718
AleI CACNNNNGTG 1 cut(s) 312
AloI GAACNNNNNNTCC 2 cut(s) 1085, 1117
AluBI AGCT 8 cut(s) 206, 436, 493, 551, 619, 809, 1127, 1152
AluI AGCT 8 cut(s) 206, 436, 493, 551, 619, 809, 1127, 1152
Alw21I GWGCWC 1 cut(s) 621
Alw26I GTCTC 1 cut(s) 831
AlwI GGATC 4 cut(s) 968, 981, 1078, 1130
AlwNI CAGNNNCTG 1 cut(s) 773
AoxI GGCC 1 cut(s) 73
ApeKI GCWGC 6 cut(s) 128, 171, 233, 818, 932, 1115
ApoI RAATTY 2 cut(s) 632, 842
AsiGI ACCGGT 1 cut(s) 755
AspS9I GGNCC 2 cut(s) 254, 304
AsuC2I CCSGG 1 cut(s) 706
AsuHPI GGTGA 3 cut(s) 650, 788, 1001
AsuII TTCGAA 1 cut(s) 225
AvaII GGWCC 2 cut(s) 254, 304
BamHI GGATCC 1 cut(s) 973
BanI GGYRCC 1 cut(s) 715
BanII GRGCYC 1 cut(s) 621
BarI GAAGNNNNNNTAC 2 cut(s) 142, 174
Bbv12I GWGCWC 1 cut(s) 621
BbvI GCAGC 6 cut(s) 140, 183, 220, 805, 919, 1127
BccI CCATC 2 cut(s) 885, 957
BcgI CGANNNNNNTGC 2 cut(s) 215, 249
BciT130I CCWGG 2 cut(s) 455, 720
BciVI GTATCC 2 cut(s) 297, 532
BclI TGATCA 1 cut(s) 331
BcnI CCSGG 1 cut(s) 706
BcoDI GTCTC 1 cut(s) 831
BfaI CTAG 3 cut(s) 414, 497, 806
BfuAI ACCTGC 1 cut(s) 176
BfuI GTATCC 2 cut(s) 297, 532
BglII AGATCT 1 cut(s) 1010
Bme1390I CCNGG 3 cut(s) 455, 706, 720
Bme18I GGWCC 2 cut(s) 254, 304
BmeRI GACNNNNNGTC 1 cut(s) 108
BmgBI CACGTC 1 cut(s) 1003
BmgT120I GGNCC 2 cut(s) 254, 304
BmiI GGNNCC 2 cut(s) 717, 975
BmrFI CCNGG 3 cut(s) 455, 706, 720
BmsI GCATC 3 cut(s) 63, 376, 919
Bpu10I CCTNAGC 2 cut(s) 578, 1140
Bpu14I TTCGAA 1 cut(s) 225
BpuMI CCSGG 1 cut(s) 706
Bsa29I ATCGAT 1 cut(s) 1027
BsaAI YACGTR 1 cut(s) 149
BsaBI GATNNNNATC 1 cut(s) 336
BsaHI GRCGYC 1 cut(s) 270
BsaJI CCNNGG 3 cut(s) 210, 705, 958
BsaWI WCCGGW 3 cut(s) 45, 755, 1103
BsaXI ACNNNNNCTCC 2 cut(s) 1085, 1115
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 220
Bse3DI GCAATG 1 cut(s) 951
Bse8I GATNNNNATC 1 cut(s) 336
BseBI CCWGG 2 cut(s) 455, 720
BseCI ATCGAT 1 cut(s) 1027
BseDI CCNNGG 3 cut(s) 210, 705, 958
BseGI GGATG 4 cut(s) 54, 391, 896, 968
BseJI GATNNNNATC 1 cut(s) 336
BseMI GCAATG 1 cut(s) 951
BseMII CTCAG 2 cut(s) 428, 765
BseNI ACTGG 1 cut(s) 220
BseXI GCAGC 6 cut(s) 140, 183, 220, 805, 919, 1127
BsgI GTGCAG 1 cut(s) 918
Bsh1236I CGCG 1 cut(s) 212
BshFI GGCC 1 cut(s) 75
BshNI GGYRCC 1 cut(s) 715
BshTI ACCGGT 1 cut(s) 755
BshVI ATCGAT 1 cut(s) 1027
BsiHKAI GWGCWC 1 cut(s) 621
BsiSI CCGG 5 cut(s) 46, 182, 705, 756, 1104
BslFI GGGAC 4 cut(s) 115, 214, 281, 784
BsmAI GTCTC 1 cut(s) 831
BsmFI GGGAC 4 cut(s) 115, 214, 281, 784
BsnI GGCC 1 cut(s) 75
Bsp119I TTCGAA 1 cut(s) 225
Bsp1286I GDGCHC 1 cut(s) 621
Bsp143I GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
BspACI CCGC 7 cut(s) 169, 210, 212, 674, 816, 998, 1118
BspANI GGCC 1 cut(s) 75
BspCNI CTCAG 2 cut(s) 429, 766
BspDI ATCGAT 1 cut(s) 1027
BspFNI CGCG 1 cut(s) 212
BspHI TCATGA 1 cut(s) 1005
BspLI GGNNCC 2 cut(s) 717, 975
BspMI ACCTGC 1 cut(s) 176
BspPI GGATC 4 cut(s) 968, 981, 1078, 1130
BspT104I TTCGAA 1 cut(s) 225
BspT107I GGYRCC 1 cut(s) 715
BsrDI GCAATG 1 cut(s) 951
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 1 cut(s) 220
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 3 cut(s) 210, 705, 958
BssMI GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
BssNI GRCGYC 1 cut(s) 270
Bst2UI CCWGG 2 cut(s) 455, 720
Bst4CI ACNGT 4 cut(s) 258, 420, 569, 880
Bst6I CTCTTC 1 cut(s) 552
BstACI GRCGYC 1 cut(s) 270
BstBAI YACGTR 1 cut(s) 149
BstBI TTCGAA 1 cut(s) 225
BstDEI CTNAG 5 cut(s) 89, 437, 578, 774, 1140
BstDSI CCRYGG 1 cut(s) 210
BstF5I GGATG 4 cut(s) 54, 391, 896, 968
BstFNI CGCG 1 cut(s) 212
BstKTI GATC 6 cut(s) 300, 334, 976, 1013, 1073, 1138
BstMAI GTCTC 1 cut(s) 831
BstMBI GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
BstMWI GCNNNNNNNGC 5 cut(s) 212, 759, 815, 941, 1124
BstNI CCWGG 2 cut(s) 455, 720
BstNSI RCATGY 1 cut(s) 1185
BstSCI CCNGG 3 cut(s) 453, 704, 718
BstUI CGCG 1 cut(s) 212
BstV1I GCAGC 6 cut(s) 140, 183, 220, 805, 919, 1127
BstX2I RGATCY 2 cut(s) 973, 1010
BstXI CCANNNNNNTGG 1 cut(s) 314
BstYI RGATCY 2 cut(s) 973, 1010
Bsu15I ATCGAT 1 cut(s) 1027
BsuI GTATCC 2 cut(s) 297, 532
BsuRI GGCC 1 cut(s) 75
BsuTUI ATCGAT 1 cut(s) 1027
BtgI CCRYGG 1 cut(s) 210
BtrI CACGTC 1 cut(s) 1003
BtsCI GGATG 4 cut(s) 54, 391, 896, 968
BtsI GCAGTG 1 cut(s) 319
BtsIMutI CAGTG 1 cut(s) 319
BveI ACCTGC 1 cut(s) 176
CaiI CAGNNNCTG 1 cut(s) 773
CciI TCATGA 1 cut(s) 1005
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 2 cut(s) 254, 304
Cfr42I CCGCGG 1 cut(s) 213
ClaI ATCGAT 1 cut(s) 1027
CseI GACGC 2 cut(s) 278, 676
Csp6I GTAC 4 cut(s) 60, 96, 346, 832
CspAI ACCGGT 1 cut(s) 755
CviAII CATG 6 cut(s) 376, 408, 812, 986, 1006, 1182
CviQI GTAC 4 cut(s) 60, 96, 346, 832
DdeI CTNAG 5 cut(s) 89, 437, 578, 774, 1140
DpnI GATC 6 cut(s) 299, 333, 975, 1012, 1072, 1137
DpnII GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
DraIII CACNNNGTG 1 cut(s) 989
DriI GACNNNNNGTC 1 cut(s) 108
Eam1104I CTCTTC 1 cut(s) 552
Eam1105I GACNNNNNGTC 1 cut(s) 108
EarI CTCTTC 1 cut(s) 552
Ecl136II GAGCTC 1 cut(s) 619
Eco24I GRGCYC 1 cut(s) 621
Eco47I GGWCC 2 cut(s) 254, 304
Eco53kI GAGCTC 1 cut(s) 619
Eco57I CTGAAG 1 cut(s) 148
EcoICRI GAGCTC 1 cut(s) 619
EcoRII CCWGG 2 cut(s) 453, 718
EcoT38I GRGCYC 1 cut(s) 621
FaeI CATG 6 cut(s) 379, 411, 815, 989, 1009, 1185
FaqI GGGAC 4 cut(s) 115, 214, 281, 784
FatI CATG 6 cut(s) 375, 407, 811, 985, 1005, 1181
FauNDI CATATG 1 cut(s) 1059
FbaI TGATCA 1 cut(s) 331
FokI GGATG 4 cut(s) 41, 398, 903, 975
FriOI GRGCYC 1 cut(s) 621
FspBI CTAG 3 cut(s) 414, 497, 806
HaeIII GGCC 1 cut(s) 75
HapII CCGG 5 cut(s) 46, 182, 705, 756, 1104
HgaI GACGC 2 cut(s) 278, 676
Hin1I GRCGYC 1 cut(s) 270
Hin1II CATG 6 cut(s) 379, 411, 815, 989, 1009, 1185
HindIII AAGCTT 1 cut(s) 549
HinfI GANTC 7 cut(s) 396, 462, 478, 595, 764, 911, 1163
HpaII CCGG 5 cut(s) 46, 182, 705, 756, 1104
HphI GGTGA 3 cut(s) 650, 788, 1001
Hpy166II GTNNAC 4 cut(s) 451, 832, 883, 1041
Hpy188I TCNGA 2 cut(s) 910, 962
Hpy188III TCNNGA 5 cut(s) 199, 290, 414, 605, 1006
Hpy8I GTNNAC 4 cut(s) 451, 832, 883, 1041
HpyAV CCTTC 7 cut(s) 172, 221, 236, 364, 452, 820, 853
HpyCH4III ACNGT 4 cut(s) 258, 420, 569, 880
HpyCH4IV ACGT 3 cut(s) 148, 519, 1002
HpyCH4V TGCA 7 cut(s) 4, 236, 614, 701, 753, 935, 1115
HpyF10VI GCNNNNNNNGC 5 cut(s) 212, 759, 815, 941, 1124
HpyF3I CTNAG 5 cut(s) 89, 437, 578, 774, 1140
HpySE526I ACGT 3 cut(s) 148, 519, 1002
Hsp92I GRCGYC 1 cut(s) 270
Hsp92II CATG 6 cut(s) 379, 411, 815, 989, 1009, 1185
Ksp22I TGATCA 1 cut(s) 331
KspI CCGCGG 1 cut(s) 213
Kzo9I GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
Lsp1109I GCAGC 6 cut(s) 140, 183, 220, 805, 919, 1127
LweI GCATC 3 cut(s) 63, 376, 919
MaeI CTAG 3 cut(s) 414, 497, 806
MaeII ACGT 3 cut(s) 148, 519, 1002
MaeIII GTNAC 9 cut(s) 144, 149, 420, 520, 638, 789, 920, 979, 989
MalI GATC 6 cut(s) 299, 333, 975, 1012, 1072, 1137
MboI GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
MboII GAAGA 3 cut(s) 307, 365, 569
MflI RGATCY 2 cut(s) 973, 1010
MhlI GDGCHC 1 cut(s) 621
MluCI AATT 5 cut(s) 13, 588, 632, 748, 842
MlyI GAGTC 2 cut(s) 905, 1157
MmeI TCCRAC 2 cut(s) 933, 1000
MnlI CCTC 7 cut(s) 86, 245, 437, 553, 594, 968, 1178
MseI TTAA 1 cut(s) 1173
MslI CAYNNNNRTG 1 cut(s) 312
MspA1I CMGCKG 3 cut(s) 212, 818, 1118
MspI CCGG 5 cut(s) 46, 182, 705, 756, 1104
MspR9I CCNGG 3 cut(s) 455, 706, 720
MvaI CCWGG 2 cut(s) 455, 720
MvnI CGCG 1 cut(s) 212
MwoI GCNNNNNNNGC 5 cut(s) 212, 759, 815, 941, 1124
NciI CCSGG 1 cut(s) 706
NdeI CATATG 1 cut(s) 1059
NdeII GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
NlaIII CATG 6 cut(s) 379, 411, 815, 989, 1009, 1185
NlaIV GGNNCC 2 cut(s) 717, 975
NmuCI GTSAC 6 cut(s) 144, 520, 638, 789, 920, 989
NspI RCATGY 1 cut(s) 1185
NspV TTCGAA 1 cut(s) 225
OliI CACNNNNGTG 1 cut(s) 312
PagI TCATGA 1 cut(s) 1005
PciI ACATGT 1 cut(s) 1181
PfeI GAWTC 5 cut(s) 396, 462, 478, 595, 764
PflFI GACNNNGTC 1 cut(s) 644
PinAI ACCGGT 1 cut(s) 755
PleI GAGTC 2 cut(s) 905, 1157
PpsI GAGTC 2 cut(s) 905, 1157
Ppu21I YACGTR 1 cut(s) 149
PscI ACATGT 1 cut(s) 1181
Psp124BI GAGCTC 1 cut(s) 621
Psp6I CCWGG 2 cut(s) 453, 718
PspGI CCWGG 2 cut(s) 453, 718
PspN4I GGNNCC 2 cut(s) 717, 975
PspPI GGNCC 2 cut(s) 254, 304
PstNI CAGNNNCTG 1 cut(s) 773
PsuI RGATCY 2 cut(s) 973, 1010
PsyI GACNNNGTC 1 cut(s) 644
RsaI GTAC 4 cut(s) 61, 97, 347, 833
RsaNI GTAC 4 cut(s) 60, 96, 346, 832
RseI CAYNNNNRTG 1 cut(s) 312
SacI GAGCTC 1 cut(s) 621
SacII CCGCGG 1 cut(s) 213
SaqAI TTAA 1 cut(s) 1173
Sau3AI GATC 6 cut(s) 297, 331, 973, 1010, 1070, 1135
Sau96I GGNCC 2 cut(s) 254, 304
SchI GAGTC 2 cut(s) 905, 1157
ScrFI CCNGG 3 cut(s) 455, 706, 720
SduI GDGCHC 1 cut(s) 621
SfaNI GCATC 3 cut(s) 63, 376, 919
Sfr303I CCGCGG 1 cut(s) 213
SfuI TTCGAA 1 cut(s) 225
SgrAI CRCCGGYG 1 cut(s) 755
SgrBI CCGCGG 1 cut(s) 213
SinI GGWCC 2 cut(s) 254, 304
SmiMI CAYNNNNRTG 1 cut(s) 312
Sse9I AATT 5 cut(s) 13, 588, 632, 748, 842
SsiI CCGC 7 cut(s) 169, 210, 212, 674, 816, 998, 1118
SspI AATATT 1 cut(s) 470
SspMI CTAG 3 cut(s) 414, 497, 806
SstI GAGCTC 1 cut(s) 621
StyD4I CCNGG 3 cut(s) 453, 704, 718
TaaI ACNGT 4 cut(s) 258, 420, 569, 880
TaiI ACGT 3 cut(s) 151, 522, 1005
TaqI TCGA 2 cut(s) 225, 1027
TasI AATT 5 cut(s) 13, 588, 632, 748, 842
TatI WGTACW 1 cut(s) 95
TauI GCSGC 4 cut(s) 171, 215, 676, 818
TfiI GAWTC 5 cut(s) 396, 462, 478, 595, 764
Tru1I TTAA 1 cut(s) 1173
Tru9I TTAA 1 cut(s) 1173
TscAI CASTG 1 cut(s) 319
TseFI GTSAC 6 cut(s) 144, 520, 638, 789, 920, 989
TseI GCWGC 6 cut(s) 128, 171, 233, 818, 932, 1115
Tsp45I GTSAC 6 cut(s) 144, 520, 638, 789, 920, 989
TspDTI ATGAA 2 cut(s) 181, 521
TspGWI ACGGA 1 cut(s) 47
TspRI CASTG 1 cut(s) 319
Tth111I GACNNNGTC 1 cut(s) 644
VpaK11BI GGWCC 2 cut(s) 254, 304
XapI RAATTY 2 cut(s) 632, 842
XbaI TCTAGA 1 cut(s) 413
XceI RCATGY 1 cut(s) 1185
XspI CTAG 3 cut(s) 414, 497, 806
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.