pycom02g06140

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr2
OG0000117
Physical Location & Seq
Reverse (-)
4153470 .. 4156718
3249 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom02g06140.7

Sequence Viewer

Length: 1614 bp
ATGGCAATGCATGATTTAGGCTCTTTGCTCTTGTGTGTCTTGCTACTCAATGGCTTTGCATTGACAAACACCAAAGCTGCTAAGCCAGATAAACCCATTGTCTGTAACTCACTTGACAGGACCAAGTTTGATGCTCTAAAACCGGGGTTCGTCTTTGGTGGATCTACAGCAGCTTACCAGGTAGAAGGTGCATGGAACGTAGATGGTAGAGGACCAAGCATATGGGACACCTTCACCCACAACCATCCAGAAAAAATCGATGATCACAGCAATGGAGATGTCGCCATTGATCAATACCACCTCTATAAGAAAGATGTAGCAATTATGAAGGATATGAAGTTGGATGCTTATAGGTTCTCTATCTCATGGCCCAGATTGTTACCAAATGGCACGCTAAGTGGGGGTGTCAACAGGAAGGGAATTGAATACTACGACAATCTCATCAATGAACTCCTTCGCAATGGCATACAGCCATTTGTGACAATCTTTCACTGGGATGTTCCCCAAGCGTTAGAAGATGCATATGGTGGTTTGTTAAGCCCTCGTATTGTCGATGACTTTAAAGACTACGCAGAACTTTGTTTTTCACTTTTTGGTGATCGAGTGAAGCACTGGATCACATTGAATGAACCATATACCATCAGTAACCATGGTTATACAATCGGGATGTACGCACCGGGACGATGCTCTGCTTGGCAAGACCCAACCTGCCTTGGTGGAGATTCGGCTACTGAACCCTATTTGGTAACACACTACCAACTCCTTGCTCATGCAGCTGCTGTAAAAGTGTACAAAGATAAATTTCAGGCATATCAAAATGGGGTGATAGGAATAACACTATTGTCACATTGGTTTGAGCCTGCCTCAGATGCAAAGGAAGATATAGATGCTGCAAATCGAGCTATGGATTTTATGTTTGGATGGTTTATGGATCCAATTACAAGAGGCGACTACCCGTACACCATGCGAGGCCTTGTTAAAGAACGATTGCCAAAATTCACGGAAGAAGAATCCAAGATGTTAACTGGGTCTTTTGATTTTGTTGGATTGAACTATTATACTGCTCTATATGCAACTGATGTACCTAAGAATTATTCTAAACCTGCAAGTTACTTATACGATCCACATGTTATTACACTGACTGAACGTGATGGCATTCCTATTGGTCCTCAGGCTGCTTCAGACTGGTTATATGTTTATCCGAAAGGAATTCACGATTTTATACTCTACACGAAGAATAAGTATGGTGATCCAATCATTTACATTACTGAGAATGGCGTTGATGAGTTCAATGATTCCAACTTATCACTAGATGATGCCCTCCATGATCCATTTAGGATTGACTACTACAATCGCCACCTTTGTTACGTTCATGCAGCAATCAAGAAGGGTAGTAATGTGAAAGGATACTTTGCATGGGCAATTTTAGACAACTTTCAATGGAGTGAAGGCTACACAGTTCGATTTGGTATTAACTATGTGGATTTTGACGGACTCCAAAGGTACCCAAAAAATTCGACCCATTGGTTCACAAATTTCCTCAAGAAGCGCAAAGGAAGTTCAAATATTTTGGCCAATAATGTTGGAGACACTGATTTTCTCTATCAAGTGTAA

Protein Analysis

538

Amino Acids

61.08

Weight (kDa)

5.63

Isoelectric Point (pI)

21.96

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
Acc36I ACCTGC 2 cut(s) 716, 1111
Acc65I GGTACC 1 cut(s) 1503
AccB1I GGYRCC 1 cut(s) 1503
AclWI GGATC 7 cut(s) 169, 623, 926, 939, 1115, 1244, 1322
AcoI YGGCCR 1 cut(s) 1572
AcsI RAATTY 5 cut(s) 800, 995, 1209, 1513, 1534
AcuI CTGAAG 1 cut(s) 1164
AfaI GTAC 5 cut(s) 671, 791, 959, 1083, 1505
AflIII ACRYGT 1 cut(s) 1126
AgsI TTSAA 6 cut(s) 425, 625, 1051, 1291, 1439, 1563
AjnI CCWGG 1 cut(s) 177
AluBI AGCT 4 cut(s) 77, 173, 776, 902
AluI AGCT 4 cut(s) 77, 173, 776, 902
Alw26I GTCTC 1 cut(s) 1581
AlwI GGATC 7 cut(s) 169, 623, 926, 939, 1115, 1244, 1322
AlwNI CAGNNNCTG 1 cut(s) 779
AoxI GGCC 3 cut(s) 368, 970, 1572
ApeKI GCWGC 7 cut(s) 77, 170, 773, 776, 890, 1175, 1376
ApoI RAATTY 5 cut(s) 800, 995, 1209, 1513, 1534
Asp700I GAANNNNTTC 1 cut(s) 453
Asp718I GGTACC 1 cut(s) 1503
AspLEI GCGC 1 cut(s) 1551
AspS9I GGNCC 4 cut(s) 120, 212, 369, 1166
AsuC2I CCSGG 2 cut(s) 144, 678
AsuHPI GGTGA 4 cut(s) 226, 608, 835, 1259
AvaII GGWCC 3 cut(s) 120, 212, 1166
AxyI CCTNAGG 1 cut(s) 1170
BaeI ACNNNNGTAYC 2 cut(s) 1065, 1098
BalI TGGCCA 1 cut(s) 1574
BamHI GGATCC 1 cut(s) 931
BanI GGYRCC 1 cut(s) 1503
BbvI GCAGC 7 cut(s) 64, 182, 763, 785, 877, 1162, 1388
BccI CCATC 5 cut(s) 197, 252, 647, 915, 1145
BciT130I CCWGG 1 cut(s) 179
BciVI GTATCC 1 cut(s) 1400
BclI TGATCA 2 cut(s) 262, 289
BcnI CCSGG 2 cut(s) 144, 678
BcoDI GTCTC 1 cut(s) 1581
BfaI CTAG 1 cut(s) 1310
BfmI CTRYAG 1 cut(s) 165
BfuAI ACCTGC 2 cut(s) 716, 1111
BfuI GTATCC 1 cut(s) 1400
BisI GCNGC 7 cut(s) 78, 171, 774, 777, 891, 1176, 1377
BlpI GCTNAGC 1 cut(s) 81
BlsI GCNGC 7 cut(s) 79, 172, 775, 778, 892, 1177, 1378
Bme1390I CCNGG 3 cut(s) 144, 179, 678
Bme18I GGWCC 3 cut(s) 120, 212, 1166
BmgT120I GGNCC 4 cut(s) 120, 212, 369, 1166
BmiI GGNNCC 2 cut(s) 933, 1505
BmrFI CCNGG 3 cut(s) 144, 179, 678
BmrI ACTGGG 2 cut(s) 502, 1035
BmsI GCATC 7 cut(s) 121, 334, 508, 674, 859, 877, 1306
BmuI ACTGGG 2 cut(s) 502, 1035
BplI GAGNNNNNCTC 2 cut(s) 848, 880
Bpu1102I GCTNAGC 1 cut(s) 81
BpuEI CTTGAG 1 cut(s) 1526
BpuMI CCSGG 2 cut(s) 144, 678
Bsa29I ATCGAT 1 cut(s) 258
BsaJI CCNNGG 3 cut(s) 143, 649, 712
Bse1I ACTGG 4 cut(s) 497, 617, 1030, 1190
Bse21I CCTNAGG 1 cut(s) 1170
Bse3DI GCAATG 3 cut(s) 12, 277, 466
BseBI CCWGG 1 cut(s) 179
BseCI ATCGAT 1 cut(s) 258
BseDI CCNNGG 3 cut(s) 143, 649, 712
BseGI GGATG 5 cut(s) 244, 349, 502, 672, 926
BseMI GCAATG 3 cut(s) 12, 277, 466
BseMII CTCAG 3 cut(s) 879, 1184, 1260
BseNI ACTGG 4 cut(s) 497, 617, 1030, 1190
BseXI GCAGC 7 cut(s) 64, 182, 763, 785, 877, 1162, 1388
BshFI GGCC 3 cut(s) 370, 972, 1574
BshNI GGYRCC 1 cut(s) 1503
BshVI ATCGAT 1 cut(s) 258
BsiSI CCGG 2 cut(s) 143, 677
BslFI GGGAC 2 cut(s) 239, 693
BsmAI GTCTC 1 cut(s) 1581
BsmFI GGGAC 2 cut(s) 239, 693
BsmI GAATGC 1 cut(s) 1155
BsnI GGCC 3 cut(s) 370, 972, 1574
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
Bsp1720I GCTNAGC 1 cut(s) 81
Bsp19I CCATGG 1 cut(s) 649
BspANI GGCC 3 cut(s) 370, 972, 1574
BspCNI CTCAG 3 cut(s) 878, 1183, 1261
BspDI ATCGAT 1 cut(s) 258
BspLI GGNNCC 2 cut(s) 933, 1505
BspMI ACCTGC 2 cut(s) 716, 1111
BspPI GGATC 7 cut(s) 169, 623, 926, 939, 1115, 1244, 1322
BspT107I GGYRCC 1 cut(s) 1503
BsrDI GCAATG 3 cut(s) 12, 277, 466
BsrGI TGTACA 1 cut(s) 789
BsrI ACTGG 4 cut(s) 497, 617, 1030, 1190
BssECI CCNNGG 3 cut(s) 143, 649, 712
BssMI GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
BssT1I CCWWGG 2 cut(s) 649, 712
Bst2UI CCWGG 1 cut(s) 179
Bst4CI ACNGT 1 cut(s) 1459
BstAUI TGTACA 1 cut(s) 789
BstC8I GCNNGC 2 cut(s) 392, 861
BstDEI CTNAG 6 cut(s) 81, 395, 865, 1086, 1170, 1269
BstDSI CCRYGG 1 cut(s) 649
BstF5I GGATG 5 cut(s) 244, 349, 502, 672, 926
BstHHI GCGC 1 cut(s) 1551
BstKTI GATC 9 cut(s) 164, 265, 292, 601, 618, 934, 1123, 1252, 1330
BstMAI GTCTC 1 cut(s) 1581
BstMBI GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
BstMWI GCNNNNNNNGC 4 cut(s) 773, 869, 899, 1070
BstNI CCWGG 1 cut(s) 179
BstNSI RCATGY 1 cut(s) 1130
BstSCI CCNGG 3 cut(s) 142, 177, 676
BstSFI CTRYAG 1 cut(s) 165
BstV1I GCAGC 7 cut(s) 64, 182, 763, 785, 877, 1162, 1388
BstX2I RGATCY 2 cut(s) 161, 931
BstXI CCANNNNNNTGG 1 cut(s) 222
BstYI RGATCY 2 cut(s) 161, 931
Bsu15I ATCGAT 1 cut(s) 258
Bsu36I CCTNAGG 1 cut(s) 1170
BsuI GTATCC 1 cut(s) 1400
BsuRI GGCC 3 cut(s) 370, 972, 1574
BsuTUI ATCGAT 1 cut(s) 258
BtgI CCRYGG 1 cut(s) 649
BtsCI GGATG 5 cut(s) 244, 349, 502, 672, 926
BtsIMutI CAGTG 4 cut(s) 490, 610, 1136, 1590
BveI ACCTGC 2 cut(s) 716, 1111
Cac8I GCNNGC 2 cut(s) 392, 861
CaiI CAGNNNCTG 1 cut(s) 779
CfoI GCGC 1 cut(s) 1551
Cfr13I GGNCC 4 cut(s) 120, 212, 369, 1166
ClaI ATCGAT 1 cut(s) 258
CsiI ACCWGGT 1 cut(s) 177
Csp6I GTAC 5 cut(s) 670, 790, 958, 1082, 1504
CviQI GTAC 5 cut(s) 670, 790, 958, 1082, 1504
DdeI CTNAG 6 cut(s) 81, 395, 865, 1086, 1170, 1269
DpnI GATC 9 cut(s) 163, 264, 291, 600, 617, 933, 1122, 1251, 1329
DpnII GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
DraI TTTAAA 1 cut(s) 562
EaeI YGGCCR 1 cut(s) 1572
Eco130I CCWWGG 2 cut(s) 649, 712
Eco147I AGGCCT 1 cut(s) 972
Eco47I GGWCC 3 cut(s) 120, 212, 1166
Eco57I CTGAAG 1 cut(s) 1164
Eco81I CCTNAGG 1 cut(s) 1170
EcoRI GAATTC 1 cut(s) 1209
EcoRII CCWGG 1 cut(s) 177
EcoT14I CCWWGG 2 cut(s) 649, 712
EcoT22I ATGCAT 2 cut(s) 12, 523
ErhI CCWWGG 2 cut(s) 649, 712
FaqI GGGAC 2 cut(s) 239, 693
FauNDI CATATG 2 cut(s) 221, 523
FbaI TGATCA 2 cut(s) 262, 289
Fnu4HI GCNGC 7 cut(s) 78, 171, 774, 777, 891, 1176, 1377
FokI GGATG 5 cut(s) 231, 356, 509, 679, 933
Fsp4HI GCNGC 7 cut(s) 78, 171, 774, 777, 891, 1176, 1377
FspBI CTAG 1 cut(s) 1310
GlaI GCGC 1 cut(s) 1550
GluI GCNGC 7 cut(s) 78, 171, 774, 777, 891, 1176, 1377
HaeIII GGCC 3 cut(s) 370, 972, 1574
HapII CCGG 2 cut(s) 143, 677
HhaI GCGC 1 cut(s) 1551
Hin6I GCGC 1 cut(s) 1549
HinP1I GCGC 1 cut(s) 1549
HincII GTYRAC 2 cut(s) 409, 1023
HindII GTYRAC 2 cut(s) 409, 1023
HinfI GANTC 4 cut(s) 722, 1010, 1295, 1494
HpaI GTTAAC 1 cut(s) 1023
HpaII CCGG 2 cut(s) 143, 677
HphI GGTGA 4 cut(s) 226, 608, 835, 1259
Hpy166II GTNNAC 5 cut(s) 409, 790, 960, 1023, 1530
Hpy188I TCNGA 3 cut(s) 868, 1183, 1203
Hpy188III TCNNGA 5 cut(s) 248, 664, 1214, 1384, 1543
Hpy8I GTNNAC 5 cut(s) 409, 790, 960, 1023, 1530
HpyAV CCTTC 7 cut(s) 179, 241, 322, 409, 464, 1381, 1442
HpyCH4III ACNGT 1 cut(s) 1459
HpyCH4IV ACGT 3 cut(s) 198, 1147, 1368
HpyF10VI GCNNNNNNNGC 4 cut(s) 773, 869, 899, 1070
HpyF3I CTNAG 6 cut(s) 81, 395, 865, 1086, 1170, 1269
HpySE526I ACGT 3 cut(s) 198, 1147, 1368
HspAI GCGC 1 cut(s) 1549
KpnI GGTACC 1 cut(s) 1507
Ksp22I TGATCA 2 cut(s) 262, 289
KspAI GTTAAC 1 cut(s) 1023
Kzo9I GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
Lsp1109I GCAGC 7 cut(s) 64, 182, 763, 785, 877, 1162, 1388
LweI GCATC 7 cut(s) 121, 334, 508, 674, 859, 877, 1306
MabI ACCWGGT 1 cut(s) 177
MaeI CTAG 1 cut(s) 1310
MaeII ACGT 3 cut(s) 198, 1147, 1368
MaeIII GTNAC 8 cut(s) 104, 378, 478, 644, 745, 843, 1109, 1364
MalI GATC 9 cut(s) 163, 264, 291, 600, 617, 933, 1122, 1251, 1329
MboI GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
MboII GAAGA 5 cut(s) 527, 890, 1016, 1019, 1246
MflI RGATCY 2 cut(s) 161, 931
MlsI TGGCCA 1 cut(s) 1574
MluNI TGGCCA 1 cut(s) 1574
MlyI GAGTC 1 cut(s) 1488
MmeI TCCRAC 4 cut(s) 321, 1024, 1323, 1564
MnlI CCTC 9 cut(s) 203, 311, 552, 874, 938, 962, 1179, 1331, 1550
Mox20I TGGCCA 1 cut(s) 1574
Mph1103I ATGCAT 2 cut(s) 12, 523
MroXI GAANNNNTTC 1 cut(s) 453
MscI TGGCCA 1 cut(s) 1574
MseI TTAA 5 cut(s) 536, 561, 978, 1022, 1473
MslI CAYNNNNRTG 2 cut(s) 270, 495
Msp20I TGGCCA 1 cut(s) 1574
MspA1I CMGCKG 1 cut(s) 776
MspI CCGG 2 cut(s) 143, 677
MspR9I CCNGG 3 cut(s) 144, 179, 678
Mva1269I GAATGC 1 cut(s) 1155
MvaI CCWGG 1 cut(s) 179
MwoI GCNNNNNNNGC 4 cut(s) 773, 869, 899, 1070
NciI CCSGG 2 cut(s) 144, 678
NcoI CCATGG 1 cut(s) 649
NdeI CATATG 2 cut(s) 221, 523
NdeII GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
NlaIV GGNNCC 2 cut(s) 933, 1505
NmuCI GTSAC 2 cut(s) 478, 843
NsiI ATGCAT 2 cut(s) 12, 523
NspI RCATGY 1 cut(s) 1130
PceI AGGCCT 1 cut(s) 972
PciI ACATGT 1 cut(s) 1126
PctI GAATGC 1 cut(s) 1155
PdmI GAANNNNTTC 1 cut(s) 453
PfeI GAWTC 3 cut(s) 722, 1010, 1295
PkrI GCNGC 7 cut(s) 79, 172, 775, 778, 892, 1177, 1378
PleI GAGTC 1 cut(s) 1488
PpsI GAGTC 1 cut(s) 1488
PscI ACATGT 1 cut(s) 1126
Psp6I CCWGG 1 cut(s) 177
PspGI CCWGG 1 cut(s) 177
PspN4I GGNNCC 2 cut(s) 933, 1505
PspPI GGNCC 4 cut(s) 120, 212, 369, 1166
PstNI CAGNNNCTG 1 cut(s) 779
PsuI RGATCY 2 cut(s) 161, 931
PvuII CAGCTG 1 cut(s) 776
RsaI GTAC 5 cut(s) 671, 791, 959, 1083, 1505
RsaNI GTAC 5 cut(s) 670, 790, 958, 1082, 1504
RseI CAYNNNNRTG 2 cut(s) 270, 495
SaqAI TTAA 5 cut(s) 536, 561, 978, 1022, 1473
SatI GCNGC 7 cut(s) 78, 171, 774, 777, 891, 1176, 1377
Sau3AI GATC 9 cut(s) 161, 262, 289, 598, 615, 931, 1120, 1249, 1327
Sau96I GGNCC 4 cut(s) 120, 212, 369, 1166
SchI GAGTC 1 cut(s) 1488
ScrFI CCNGG 3 cut(s) 144, 179, 678
SexAI ACCWGGT 1 cut(s) 177
SfaNI GCATC 7 cut(s) 121, 334, 508, 674, 859, 877, 1306
SfcI CTRYAG 1 cut(s) 165
SinI GGWCC 3 cut(s) 120, 212, 1166
SmiMI CAYNNNNRTG 2 cut(s) 270, 495
SmlI CTYRAG 1 cut(s) 1541
SmoI CTYRAG 1 cut(s) 1541
SseBI AGGCCT 1 cut(s) 972
SspI AATATT 1 cut(s) 1567
SspMI CTAG 1 cut(s) 1310
StuI AGGCCT 1 cut(s) 972
StyD4I CCNGG 3 cut(s) 142, 177, 676
StyI CCWWGG 2 cut(s) 649, 712
TaaI ACNGT 1 cut(s) 1459
TaiI ACGT 3 cut(s) 201, 1150, 1371
TaqI TCGA 6 cut(s) 258, 552, 601, 898, 1462, 1517
TatI WGTACW 1 cut(s) 789
TfiI GAWTC 3 cut(s) 722, 1010, 1295
Tru1I TTAA 5 cut(s) 536, 561, 978, 1022, 1473
Tru9I TTAA 5 cut(s) 536, 561, 978, 1022, 1473
TscAI CASTG 4 cut(s) 497, 617, 1143, 1597
TseFI GTSAC 2 cut(s) 478, 843
TseI GCWGC 7 cut(s) 77, 170, 773, 776, 890, 1175, 1376
Tsp45I GTSAC 2 cut(s) 478, 843
TspDTI ATGAA 5 cut(s) 341, 350, 462, 642, 1361
TspGWI ACGGA 2 cut(s) 1016, 1506
TspRI CASTG 4 cut(s) 497, 617, 1143, 1597
VpaK11BI GGWCC 3 cut(s) 120, 212, 1166
XapI RAATTY 5 cut(s) 800, 995, 1209, 1513, 1534
XceI RCATGY 1 cut(s) 1130
XmnI GAANNNNTTC 1 cut(s) 453
XspI CTAG 1 cut(s) 1310
Zsp2I ATGCAT 2 cut(s) 12, 523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.