RchiOBHm_Chr5g0077981

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
5
OG0000117
Physical Location & Seq
Forward (+)
83854432 .. 83859343
4912 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ35253

Sequence Viewer

Length: 1536 bp
ATGGCACTCCCTCGCACCCTCCTCTTGGGTTTCCTTCTTGTTCTTGGCTGTGCTGTGACGACTAGTAGATCGACCAACTCTAAGCAATGTGACAATGCTTCCCTCAACCGGAGCAGTTTTCCAGCAGGATTCATATTTGGGACAGCTTCGGCAGCTTACCAGTATGAAGGTGCTGCAAAAGAAGGGGGTCGAGGACCAAGTATATGGGACACATACACTCACAAGCATCCAGAGAAGATCTTTGACCACAGCACTGGAGATGTGGCTATTGATCAATATCATCGCTATAAGGAAGATGTTCGGACTATGAAGGACCTAGGTTTCGATGCTTACAGACTCTCATTCTCATGGTCCAGACTGTTACCAAATGGAAAGCTAAGTGGGGGTGTCAACAAGGAAGGAATCAAATATTACAACAACCTCATCAATGAGCTTCTAGCCAATGGTGTGAAGCCTTTTGTCACAATCTTTCATTGGGATCTTCCCCAAGCATTAGAAGATGAATACGGAGGCTTCTTAAATCCAAAAATTGTGAACCATTTTCAGGACTATGCAGAGCTCTGCTTTAAAGAATTCGGTGACAGGGTTAAGCATTGGATCACCTTAAATGAGCCCCATAGCTACAGCCGTAATGGCTATGCAACCGGAAAGTTTGCACCAGGTCGATGTTCTGCTTGGCAAAAACGAAATTGCACCGGTGGGGATTCAGGGACAGAGCCATACTTAGCGTCACATTACCAACTTCTAGCTCATGCAGCCGCTGTAAAGTTGTATAGACACGAGTATCAGGCAAAACAGAATGGCTTGATAGGGATAACACTAGTGTCACACTGGTTTGTGCCATATTCTGATTCAAAGCAAGACAAAGCTGCAGCATTACGAGCATTGGATTTTATGTTTGGATGGTATATGGATCCCATGACAAATGGTGACTATCCAAGAAGCATGCGATCTCTTGTGGGAAATCGATTACCCAAGTTCACTGCAGAGCAATCAGAGATGCTGAAAGGTTCATTCGATTTTCTTGGCTTAAACTACTACACAGGTAATTATGCAGCCAATACACCATTTCGTAATGCTAAAGATCCAAGCTACTCGACAGACTCTCTCGCTAATCTTACAGCCGTGCGTAATGGGATTCCAATTGGTCGACCGGCTGCTTCAGATTGGCTTCATTTTTATCCACAAGGGTTTATCGAGATATTGCGTTACACGAAGAAAAAGTATAACAATCCGCTTATTTATATCACTGAAAATGGATGGGATGACCGCGATGATCCCAATTTATCGCTAGAGGAAGCCCTCACCGATAATCAAAGAATCGAGTATTACAATCGCCATCTTGATGAACTTCATAAAGCAATTAAGGCTGGCGTTAAGGTGAAGGGGTACTTTGCATGGTCATTGTTTGATAACTTTGAGTGGGCTGATGGTTATAGTTGTAGGTTTGGCATCAATTTTGTGGATTACAAAAATGGACTCAAAAGACACCCTAAACTCTCCGCAGCTTGGTTCAAGGACTTTCTGCGAAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00460 ko00500 ko00940 ko01100 ko01110 map00460 map00500 map00940 map01100 map01110
Pfam Domains
Protein Families
Glyco_hydro_1

Protein Analysis

511

Amino Acids

58.27

Weight (kDa)

8.78

Isoelectric Point (pI)

30.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 38 - 510 2.8e-159 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 1150
AccII CGCG 1 cut(s) 1272
AciI CCGC 4 cut(s) 759, 1235, 1270, 1503
AclWI GGATC 6 cut(s) 486, 605, 908, 921, 1079, 1271
AcsI RAATTY 1 cut(s) 572
AcuI CTGAAG 1 cut(s) 1146
AfaI GTAC 1 cut(s) 1391
AfiI CCNNNNNNNGG 4 cut(s) 25, 108, 544, 1509
AgeI ACCGGT 1 cut(s) 695
AgsI TTSAA 2 cut(s) 855, 1516
AhlI ACTAGT 2 cut(s) 62, 820
AjnI CCWGG 1 cut(s) 658
Alw21I GWGCWC 1 cut(s) 561
AlwI GGATC 6 cut(s) 486, 605, 908, 921, 1079, 1271
AlwNI CAGNNNCTG 1 cut(s) 761
ApeKI GCWGC 8 cut(s) 152, 173, 755, 869, 872, 1055, 1157, 1505
ApoI RAATTY 1 cut(s) 572
ArsI GACNNNNNNTTYG 2 cut(s) 305, 337
AsiGI ACCGGT 1 cut(s) 695
Asp700I GAANNNNTTC 1 cut(s) 297
AspA2I CCTAGG 1 cut(s) 316
AspS9I GGNCC 3 cut(s) 194, 313, 351
AsuHPI GGTGA 5 cut(s) 590, 592, 941, 1297, 1393
AvaII GGWCC 3 cut(s) 194, 313, 351
AvrII CCTAGG 1 cut(s) 316
BaeI ACNNNNGTAYC 2 cut(s) 767, 800
BamHI GGATCC 1 cut(s) 913
BanII GRGCYC 2 cut(s) 561, 615
BauI CACGAG 1 cut(s) 779
Bbv12I GWGCWC 1 cut(s) 561
BbvI GCAGC 8 cut(s) 160, 164, 767, 856, 884, 1067, 1144, 1517
BccI CCATC 4 cut(s) 897, 1254, 1347, 1424
BceAI ACGGC 2 cut(s) 612, 1109
BciT130I CCWGG 1 cut(s) 660
BclI TGATCA 1 cut(s) 271
BcuI ACTAGT 2 cut(s) 62, 820
BfaI CTAG 6 cut(s) 63, 317, 437, 746, 821, 1292
BfmI CTRYAG 3 cut(s) 622, 870, 984
BglI GCCNNNNNGGC 1 cut(s) 633
BglII AGATCT 1 cut(s) 237
BisI GCNGC 9 cut(s) 153, 174, 756, 759, 870, 873, 1056, 1158, 1506
BlnI CCTAGG 1 cut(s) 316
BlsI GCNGC 9 cut(s) 154, 175, 757, 760, 871, 874, 1057, 1159, 1507
Bme1390I CCNGG 1 cut(s) 660
Bme18I GGWCC 3 cut(s) 194, 313, 351
BmgT120I GGNCC 3 cut(s) 194, 313, 351
BmiI GGNNCC 1 cut(s) 915
BmrFI CCNGG 1 cut(s) 660
BmsI GCATC 4 cut(s) 235, 316, 990, 1461
BpmI CTGGAG 1 cut(s) 276
Bsa29I ATCGAT 1 cut(s) 967
BsaBI GATNNNNATC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 316
BsaWI WCCGGW 3 cut(s) 108, 644, 695
Bsc4I CCNNNNNNNGG 4 cut(s) 25, 108, 544, 1509
Bse118I RCCGGY 2 cut(s) 695, 1153
Bse1I ACTGG 3 cut(s) 160, 259, 836
Bse3DI GCAATG 1 cut(s) 92
Bse8I GATNNNNATC 1 cut(s) 276
BseBI CCWGG 1 cut(s) 660
BseCI ATCGAT 1 cut(s) 967
BseDI CCNNGG 1 cut(s) 316
BseGI GGATG 4 cut(s) 226, 908, 1265, 1270
BseJI GATNNNNATC 1 cut(s) 276
BseLI CCNNNNNNNGG 4 cut(s) 25, 108, 544, 1509
BseMI GCAATG 1 cut(s) 92
BseNI ACTGG 3 cut(s) 160, 259, 836
BseRI GAGGAG 1 cut(s) 11
BseXI GCAGC 8 cut(s) 160, 164, 767, 856, 884, 1067, 1144, 1517
Bsh1236I CGCG 1 cut(s) 1272
Bsh1285I CGRYCG 1 cut(s) 1154
BshTI ACCGGT 1 cut(s) 695
BshVI ATCGAT 1 cut(s) 967
BsiEI CGRYCG 1 cut(s) 1154
BsiHKAI GWGCWC 1 cut(s) 561
BsiSI CCGG 4 cut(s) 109, 645, 696, 1154
BslFI GGGAC 3 cut(s) 154, 221, 724
BslI CCNNNNNNNGG 4 cut(s) 25, 108, 544, 1509
BsmFI GGGAC 3 cut(s) 154, 221, 724
Bsp1286I GDGCHC 2 cut(s) 561, 615
Bsp143I GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
BspACI CCGC 4 cut(s) 759, 1235, 1270, 1503
BspDI ATCGAT 1 cut(s) 967
BspFNI CGCG 1 cut(s) 1272
BspLI GGNNCC 1 cut(s) 915
BspMAI CTGCAG 2 cut(s) 874, 988
BspPI GGATC 6 cut(s) 486, 605, 908, 921, 1079, 1271
BsrDI GCAATG 1 cut(s) 92
BsrFI RCCGGY 2 cut(s) 695, 1153
BsrI ACTGG 3 cut(s) 160, 259, 836
BssAI RCCGGY 2 cut(s) 695, 1153
BssECI CCNNGG 1 cut(s) 316
BssMI GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
BssSI CACGAG 1 cut(s) 779
BssT1I CCWWGG 1 cut(s) 316
Bst2BI CACGAG 1 cut(s) 779
Bst2UI CCWGG 1 cut(s) 660
Bst4CI ACNGT 1 cut(s) 360
BstC8I GCNNGC 2 cut(s) 947, 1372
BstDEI CTNAG 3 cut(s) 81, 377, 724
BstF5I GGATG 4 cut(s) 226, 908, 1265, 1270
BstFNI CGCG 1 cut(s) 1272
BstKTI GATC 9 cut(s) 71, 240, 274, 481, 600, 916, 953, 1087, 1279
BstMBI GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
BstMCI CGRYCG 1 cut(s) 1154
BstMWI GCNNNNNNNGC 5 cut(s) 152, 633, 755, 881, 1367
BstNI CCWGG 1 cut(s) 660
BstNSI RCATGY 1 cut(s) 949
BstSCI CCNGG 1 cut(s) 658
BstSFI CTRYAG 3 cut(s) 622, 870, 984
BstUI CGCG 1 cut(s) 1272
BstV1I GCAGC 8 cut(s) 160, 164, 767, 856, 884, 1067, 1144, 1517
BstX2I RGATCY 4 cut(s) 237, 478, 913, 1084
BstXI CCANNNNNNTGG 2 cut(s) 204, 254
BstYI RGATCY 4 cut(s) 237, 478, 913, 1084
Bsu15I ATCGAT 1 cut(s) 967
BsuTUI ATCGAT 1 cut(s) 967
BtgZI GCGATG 2 cut(s) 266, 1287
BtsCI GGATG 4 cut(s) 226, 908, 1265, 1270
BtsI GCAGTG 1 cut(s) 981
BtsIMutI CAGTG 4 cut(s) 252, 829, 981, 1248
Cac8I GCNNGC 2 cut(s) 947, 1372
CaiI CAGNNNCTG 1 cut(s) 761
Cfr10I RCCGGY 2 cut(s) 695, 1153
Cfr13I GGNCC 3 cut(s) 194, 313, 351
ClaI ATCGAT 1 cut(s) 967
CseI GACGC 1 cut(s) 717
CsiI ACCWGGT 1 cut(s) 658
Csp6I GTAC 1 cut(s) 1390
CspAI ACCGGT 1 cut(s) 695
CviAII CATG 5 cut(s) 348, 752, 919, 946, 1398
CviQI GTAC 1 cut(s) 1390
DdeI CTNAG 3 cut(s) 81, 377, 724
DpnI GATC 9 cut(s) 70, 239, 273, 480, 599, 915, 952, 1086, 1278
DpnII GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
DraI TTTAAA 1 cut(s) 568
Ecl136II GAGCTC 1 cut(s) 559
Eco130I CCWWGG 1 cut(s) 316
Eco24I GRGCYC 2 cut(s) 561, 615
Eco47I GGWCC 3 cut(s) 194, 313, 351
Eco53kI GAGCTC 1 cut(s) 559
Eco57I CTGAAG 1 cut(s) 1146
EcoICRI GAGCTC 1 cut(s) 559
EcoO109I RGGNCCY 1 cut(s) 313
EcoRI GAATTC 1 cut(s) 572
EcoRII CCWGG 1 cut(s) 658
EcoT14I CCWWGG 1 cut(s) 316
EcoT38I GRGCYC 2 cut(s) 561, 615
ErhI CCWWGG 1 cut(s) 316
FaeI CATG 5 cut(s) 351, 755, 922, 949, 1401
FalI AAGNNNNNCTT 2 cut(s) 1376, 1408
FaqI GGGAC 3 cut(s) 154, 221, 724
FatI CATG 5 cut(s) 347, 751, 918, 945, 1397
FbaI TGATCA 1 cut(s) 271
FblI GTMKAC 1 cut(s) 1150
Fnu4HI GCNGC 9 cut(s) 153, 174, 756, 759, 870, 873, 1056, 1158, 1506
FokI GGATG 4 cut(s) 213, 915, 1272, 1277
FriOI GRGCYC 2 cut(s) 561, 615
Fsp4HI GCNGC 9 cut(s) 153, 174, 756, 759, 870, 873, 1056, 1158, 1506
FspBI CTAG 6 cut(s) 63, 317, 437, 746, 821, 1292
GluI GCNGC 9 cut(s) 153, 174, 756, 759, 870, 873, 1056, 1158, 1506
GsuI CTGGAG 1 cut(s) 276
HapII CCGG 4 cut(s) 109, 645, 696, 1154
HgaI GACGC 1 cut(s) 717
Hin1II CATG 5 cut(s) 351, 755, 922, 949, 1401
HincII GTYRAC 2 cut(s) 391, 1151
HindII GTYRAC 2 cut(s) 391, 1151
HinfI GANTC 9 cut(s) 129, 336, 402, 704, 851, 1103, 1138, 1320, 1479
HpaII CCGG 4 cut(s) 109, 645, 696, 1154
HphI GGTGA 5 cut(s) 590, 592, 941, 1297, 1393
Hpy166II GTNNAC 4 cut(s) 391, 535, 981, 1151
Hpy188I TCNGA 4 cut(s) 303, 850, 997, 1165
Hpy188III TCNNGA 5 cut(s) 230, 354, 545, 1198, 1343
Hpy8I GTNNAC 4 cut(s) 391, 535, 981, 1151
HpyAV CCTTC 6 cut(s) 44, 161, 176, 304, 392, 1378
HpyCH4III ACNGT 1 cut(s) 360
HpyF10VI GCNNNNNNNGC 5 cut(s) 152, 633, 755, 881, 1367
HpyF3I CTNAG 3 cut(s) 81, 377, 724
Hsp92II CATG 5 cut(s) 351, 755, 922, 949, 1401
Ksp22I TGATCA 1 cut(s) 271
Kzo9I GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
LmnI GCTCC 1 cut(s) 111
Lsp1109I GCAGC 8 cut(s) 160, 164, 767, 856, 884, 1067, 1144, 1517
LweI GCATC 4 cut(s) 235, 316, 990, 1461
MabI ACCWGGT 1 cut(s) 658
MaeI CTAG 6 cut(s) 63, 317, 437, 746, 821, 1292
MaeIII GTNAC 9 cut(s) 55, 89, 360, 460, 578, 729, 825, 929, 1208
MalI GATC 9 cut(s) 70, 239, 273, 480, 599, 915, 952, 1086, 1278
MboI GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
MboII GAAGA 5 cut(s) 247, 305, 473, 509, 1228
MfeI CAATTG 1 cut(s) 1143
MflI RGATCY 4 cut(s) 237, 478, 913, 1084
MhlI GDGCHC 2 cut(s) 561, 615
MluCI AATT 8 cut(s) 528, 572, 688, 1048, 1143, 1282, 1362, 1456
MlyI GAGTC 3 cut(s) 330, 1097, 1473
MnlI CCTC 9 cut(s) 21, 29, 32, 113, 185, 431, 503, 1288, 1313
MroXI GAANNNNTTC 1 cut(s) 297
MseI TTAA 7 cut(s) 518, 567, 588, 605, 1031, 1365, 1377
MslI CAYNNNNRTG 2 cut(s) 346, 1344
MspA1I CMGCKG 1 cut(s) 761
MspI CCGG 4 cut(s) 109, 645, 696, 1154
MspR9I CCNGG 1 cut(s) 660
MunI CAATTG 1 cut(s) 1143
MvaI CCWGG 1 cut(s) 660
MvnI CGCG 1 cut(s) 1272
MwoI GCNNNNNNNGC 5 cut(s) 152, 633, 755, 881, 1367
NdeII GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
NlaIII CATG 5 cut(s) 351, 755, 922, 949, 1401
NlaIV GGNNCC 1 cut(s) 915
NmuCI GTSAC 7 cut(s) 55, 89, 460, 578, 729, 825, 929
NspI RCATGY 1 cut(s) 949
PaeI GCATGC 1 cut(s) 949
PdmI GAANNNNTTC 1 cut(s) 297
PfeI GAWTC 6 cut(s) 129, 402, 704, 851, 1138, 1320
PinAI ACCGGT 1 cut(s) 695
PkrI GCNGC 9 cut(s) 154, 175, 757, 760, 871, 874, 1057, 1159, 1507
PleI GAGTC 3 cut(s) 330, 1097, 1473
PpsI GAGTC 3 cut(s) 330, 1097, 1473
PpuMI RGGWCCY 1 cut(s) 313
Psp124BI GAGCTC 1 cut(s) 561
Psp5II RGGWCCY 1 cut(s) 313
Psp6I CCWGG 1 cut(s) 658
PspGI CCWGG 1 cut(s) 658
PspN4I GGNNCC 1 cut(s) 915
PspPI GGNCC 3 cut(s) 194, 313, 351
PspPPI RGGWCCY 1 cut(s) 313
PstI CTGCAG 2 cut(s) 874, 988
PstNI CAGNNNCTG 1 cut(s) 761
PsuI RGATCY 4 cut(s) 237, 478, 913, 1084
RsaI GTAC 1 cut(s) 1391
RsaNI GTAC 1 cut(s) 1390
RseI CAYNNNNRTG 2 cut(s) 346, 1344
SacI GAGCTC 1 cut(s) 561
SalI GTCGAC 1 cut(s) 1149
SaqAI TTAA 7 cut(s) 518, 567, 588, 605, 1031, 1365, 1377
SatI GCNGC 9 cut(s) 153, 174, 756, 759, 870, 873, 1056, 1158, 1506
Sau3AI GATC 9 cut(s) 68, 237, 271, 478, 597, 913, 950, 1084, 1276
Sau96I GGNCC 3 cut(s) 194, 313, 351
SchI GAGTC 3 cut(s) 330, 1097, 1473
ScrFI CCNGG 1 cut(s) 660
SduI GDGCHC 2 cut(s) 561, 615
SexAI ACCWGGT 1 cut(s) 658
SfaNI GCATC 4 cut(s) 235, 316, 990, 1461
SfcI CTRYAG 3 cut(s) 622, 870, 984
SgrAI CRCCGGYG 1 cut(s) 695
SinI GGWCC 3 cut(s) 194, 313, 351
SmiMI CAYNNNNRTG 2 cut(s) 346, 1344
SpeI ACTAGT 2 cut(s) 62, 820
SphI GCATGC 1 cut(s) 949
Sse9I AATT 8 cut(s) 528, 572, 688, 1048, 1143, 1282, 1362, 1456
SsiI CCGC 4 cut(s) 759, 1235, 1270, 1503
SspI AATATT 1 cut(s) 410
SspMI CTAG 6 cut(s) 63, 317, 437, 746, 821, 1292
SstI GAGCTC 1 cut(s) 561
StyD4I CCNGG 1 cut(s) 658
StyI CCWWGG 1 cut(s) 316
TaaI ACNGT 1 cut(s) 360
TasI AATT 8 cut(s) 528, 572, 688, 1048, 1143, 1282, 1362, 1456
TauI GCSGC 1 cut(s) 761
TfiI GAWTC 6 cut(s) 129, 402, 704, 851, 1138, 1320
Tru1I TTAA 7 cut(s) 518, 567, 588, 605, 1031, 1365, 1377
Tru9I TTAA 7 cut(s) 518, 567, 588, 605, 1031, 1365, 1377
TscAI CASTG 4 cut(s) 259, 836, 988, 1255
TseFI GTSAC 7 cut(s) 55, 89, 460, 578, 729, 825, 929
TseI GCWGC 8 cut(s) 152, 173, 755, 869, 872, 1055, 1157, 1505
Tsp45I GTSAC 7 cut(s) 55, 89, 460, 578, 729, 825, 929
TspDTI ATGAA 9 cut(s) 121, 180, 323, 461, 516, 1002, 1163, 1343, 1362
TspGWI ACGGA 1 cut(s) 522
TspRI CASTG 4 cut(s) 259, 836, 988, 1255
VpaK11BI GGWCC 3 cut(s) 194, 313, 351
XapI RAATTY 1 cut(s) 572
XceI RCATGY 1 cut(s) 949
XmaJI CCTAGG 1 cut(s) 316
XmiI GTMKAC 1 cut(s) 1150
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 6 cut(s) 63, 317, 437, 746, 821, 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.