MD03G1204500.v1.1

Belongs to the glycosyl hydrolase 1 family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0000117
Physical Location & Seq
Forward (+)
27933665 .. 27935526
1862 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1204500.v1.1.491

Sequence Viewer

Length: 918 bp
ATGTGTATCTACAGCGATCATTTTAAAGACTATGCGGAACTTTGTTATAAGGAATTTGGTGATCGAGTCAAGCACTGGTTCACGGAAAATGAGCCATATACTTTTAGTTACATGGGTTATGCTGTTGGGACTCAAGTACCGGGACGCTGCTCTTCTTGGCAAAACCTAAACTGCATCGGTGGAGATTCAGCCATTGAACCATACTTGGTGGCACACCACCTTCTTCTTGCTCATGGAGCAGCTGTAGAATTGTACAAGAATAAATATCAGTCTGCTTCGGATTCAAAGCAAGATAAAGATGCTGCCTTACGATCTTTGGATTTTATGTTTGCATGGCAAGTGTTTTTGGACCCATTAACAAGTGGTGACTATCCACACACCATGCGACCAATTGTTGGGAAAAGATTGCCCAAATTCACAAAAGAACAATCCAAGTTGCTAAACGGATTATTTGATTTTCTTGGAATAAATTGTTATACTGCTAGATACACAAGTAGTGCACCCAAGAACAATTCACTACCGGCAAGCTACGTAACAGATTCTCGAGCTGATCTTATCAGTGAGTGCAAATGGAAACTTACTGAGCTTAATGGAGTACTCATTGGTCCACAGGCTGCTTCAGATTGGTTATATGTATATCCAAAAGGAATTCATGATCTTGTGCTTTACACAAAGGAAAAATATAATGATCCACTCATTTATATTACTGAGAATGGTATAAATGGCGCGAAAGTGAAGGGATACTTTGCATGGACATTGTTAGACAACTTTGAATGGACTTCTGGATACACTGTCCAATTTGGTTTAAACTACGTGGATTATAAAGATGGGCTGAAAAGATACCTAAAACTCTTAACACATTGGTTCAAAAATTTGCTTAAGAAGAATAAATTCAAAGAAAGCTACAATTCATTATAA

Protein Analysis

306

Amino Acids

35.11

Weight (kDa)

8.32

Isoelectric Point (pI)

22.95

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Glyco_hydro_1 PF00232 6 - 241 5.9e-41 Glycosyl hydrolase family 1
Glyco_hydro_1 PF00232 241 - 297 6.9e-15 Glycosyl hydrolase family 1
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000117)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G47600 AT1G47600 AT1G51470 AT1G51490
fragaria_vesca FvH4_3g43490 FvH4_3g43490 FvH4_3g43490 FvH4_3g43500 FvH4_3g43501 FvH4_3g43501 FvH4_3g43502 FvH4_3g43503 FvH4_3g43504 FvH4_3g43504 FvH4_3g43504 FvH4_3g43510 FvH4_3g43520 FvH4_3g43520 FvH4_3g43520
malus_domestica MD00G1105700.v1.1 MD00G1105800.v1.1 MD00G1145200.v1.1 MD00G1145300.v1.1 MD00G1190900.v1.1 MD00G1219900.v1.1 MD03G1011300.v1.1 MD03G1021500.v1.1 MD03G1021600.v1.1 MD03G1068100.v1.1 MD03G1068200.v1.1 MD03G1069100.v1.1 MD03G1069400.v1.1 MD03G1069500.v1.1 MD03G1069600.v1.1 MD03G1098600.v1.1 MD03G1204500.v1.1 MD03G1204600.v1.1 MD03G1204700.v1.1 MD03G1204800.v1.1 MD05G1053100.v1.1 MD05G1105800.v1.1 MD05G1105900.v1.1 MD05G1106100.v1.1 MD05G1106200.v1.1 MD05G1114800.v1.1 MD05G1114900.v1.1 MD05G1121900.v1.1 MD05G1312800.v1.1 MD09G1243000.v1.1 MD09G1243300.v1.1 MD10G1124500.v1.1 MD11G1023200.v1.1 MD11G1023700.v1.1 MD11G1023900.v1.1 MD11G1024000.v1.1 MD11G1024100.v1.1 MD11G1027500.v1.1 MD11G1027700.v1.1 MD12G1211300.v1.1 MD12G1211400.v1.1 MD12G1211500.v1.1 MD15G1374300.v1.1 MD15G1441900.v1.1 MD15G1442000.v1.1 MD15G1442100.v1.1
prunus_persica Prupe.1G014500_v2.0.a1 Prupe.1G584100_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G197900_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198000_v2.0.a1 Prupe.2G198100_v2.0.a1 Prupe.2G198200_v2.0.a1 Prupe.2G198300_v2.0.a1 Prupe.2G198400_v2.0.a1 Prupe.3G110900_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.3G111000_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.4G165800_v2.0.a1 Prupe.6G018800_v2.0.a1 Prupe.6G018900_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019000_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019100_v2.0.a1 Prupe.6G019200_v2.0.a1 Prupe.6G019300_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019500_v2.0.a1 Prupe.6G019600_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019700_v2.0.a1 Prupe.6G019900_v2.0.a1 Prupe.6G020100_v2.0.a1 Prupe.6G088200_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G134900_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136100_v2.0.a1 Prupe.6G136300_v2.0.a1 Prupe.6G136400_v2.0.a1 Prupe.6G136500_v2.0.a1 Prupe.6G136700_v2.0.a1 Prupe.6G145100_v2.0.a1 Prupe.7G083600_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G083700_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105400_v2.0.a1 Prupe.7G105800_v2.0.a1 Prupe.7G106100_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.8G165700_v2.0.a1 Prupe.I005900_v2.0.a1
pyrus_communis pycom02g06130 pycom02g06140 pycom03g05540 pycom05g09690 pycom05g09730 pycom05g11590 pycom05g13130 pycom09g16130 pycom09g16140 pycom09g16150 pycom09g16160 pycom09g16190 pycom11g01890 pycom11g02100 pycom11g02130 pycom11g09390 pycom11g09400 pycom11g09490 pycom812g00010
rosa_chinensis RchiOBHm_Chr5g0071491 RchiOBHm_Chr5g0071511 RchiOBHm_Chr5g0077911 RchiOBHm_Chr5g0077951 RchiOBHm_Chr5g0077961 RchiOBHm_Chr5g0077971 RchiOBHm_Chr5g0077981 RchiOBHm_Chr5g0078001 RchiOBHm_Chr5g0078011
rosa_laevigata RLG00000036725 RLG00000036729 RLG00000036732 RLG00000036735 RLG00000036736 RLG00000036737 RLG00000036738 RLG00000036739 RLG00000036740 RLG00000036741 RLG00000036745
rosa_multiflora Rmu_co8284559.1_g000001 Rmu_co8309117.1_g000001 Rmu_sc0000177.1_g000021 Rmu_sc0001711.1_g000024 Rmu_sc0002139.1_g000001 Rmu_sc0002139.1_g000004 Rmu_sc0002139.1_g000005 Rmu_sc0004697.1_g000006 Rmu_sc0006499.1_g000016 Rmu_sc0009988.1_g000001
rosa_roxburghii Rroxscaffold_1G00000230 Rroxscaffold_1G00000260 Rroxscaffold_1G00000280 Rroxscaffold_1G00000290 Rroxscaffold_1G00004220 Rroxscaffold_1G00004280
rosa_rugosa Rorug05G0457000 Rorug05G0457100 Rorug05G0457200 Rorug05G0457300 Rorug05G0457400 Rorug05G0457500 Rorug05G0457600 Rorug05G0457700 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457800 Rorug05G0457900 Rorug05G0458000 Rorug05G0458100
rosa_samantha Rh5AG468700 Rh5AG468800 Rh5AG511700 Rh5AG512200 Rh5AG512300 Rh5AG512400 Rh5AG512700 Rh5AG512800 Rh5CG558300 Rh5CG558500 Rh5DG546000 Rh5DG546300 Rh5DG546600 Rh5DG546700 Rh5DG546900 Rh5DG547000 Rh5DG547100 Rh5DG547300 Rh6BG198200
rosa_wichuraiana Rw5G043550 Rw5G047470 Rw5G047480 Rw5G047500 Rw5G047510 Rw5G047520 Rw5G047530 Rw5G047540 Rw5G047550 Rw5G047560 Rw6G016930

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 3 cut(s) 48, 822, 916
AccB7I CCANNNNNTGG 1 cut(s) 395
AccII CGCG 1 cut(s) 728
AciI CCGC 1 cut(s) 35
AclWI GGATC 1 cut(s) 683
AcsI RAATTY 5 cut(s) 53, 413, 648, 871, 890
AcuI CTGAAG 1 cut(s) 603
AfaI GTAC 3 cut(s) 138, 254, 597
AfiI CCNNNNNNNGG 1 cut(s) 395
AflII CTTAAG 1 cut(s) 878
AgsI TTSAA 5 cut(s) 197, 285, 773, 868, 895
AluBI AGCT 5 cut(s) 242, 528, 548, 586, 903
AluI AGCT 5 cut(s) 242, 528, 548, 586, 903
Alw21I GWGCWC 1 cut(s) 502
Alw44I GTGCAC 1 cut(s) 498
AlwI GGATC 1 cut(s) 683
Ama87I CYCGRG 1 cut(s) 543
ApaLI GTGCAC 1 cut(s) 498
ApeKI GCWGC 4 cut(s) 147, 239, 302, 614
ApoI RAATTY 5 cut(s) 53, 413, 648, 871, 890
Asp700I GAANNNNTTC 1 cut(s) 890
AspLEI GCGC 1 cut(s) 728
AspS9I GGNCC 2 cut(s) 349, 605
AsuC2I CCSGG 1 cut(s) 141
AsuHPI GGTGA 2 cut(s) 71, 377
AvaI CYCGRG 1 cut(s) 543
AvaII GGWCC 2 cut(s) 349, 605
BaeGI GKGCMC 1 cut(s) 502
BaeI ACNNNNGTAYC 4 cut(s) 120, 153, 478, 511
Bbv12I GWGCWC 1 cut(s) 502
BbvI GCAGC 4 cut(s) 134, 251, 289, 601
BccI CCATC 1 cut(s) 821
BciVI GTATCC 2 cut(s) 734, 779
BcnI CCSGG 1 cut(s) 141
BfaI CTAG 1 cut(s) 483
BfmI CTRYAG 2 cut(s) 10, 243
BfrI CTTAAG 1 cut(s) 878
BfuI GTATCC 2 cut(s) 734, 779
BisI GCNGC 4 cut(s) 148, 240, 303, 615
BlsI GCNGC 4 cut(s) 149, 241, 304, 616
BmcAI AGTACT 1 cut(s) 597
Bme1390I CCNGG 1 cut(s) 141
Bme18I GGWCC 2 cut(s) 349, 605
BmeT110I CYCGRG 1 cut(s) 543
BmgT120I GGNCC 2 cut(s) 349, 605
BmiI GGNNCC 1 cut(s) 351
BmrFI CCNGG 1 cut(s) 141
BmsI GCATC 2 cut(s) 183, 289
BpuEI CTTGAG 1 cut(s) 117
BpuMI CCSGG 1 cut(s) 141
BsaAI YACGTR 2 cut(s) 532, 814
BsaXI ACNNNNNCTCC 2 cut(s) 228, 258
Bsc4I CCNNNNNNNGG 1 cut(s) 395
Bse118I RCCGGY 1 cut(s) 520
Bse1I ACTGG 1 cut(s) 80
BseLI CCNNNNNNNGG 1 cut(s) 395
BseMII CTCAG 2 cut(s) 573, 699
BseNI ACTGG 1 cut(s) 80
BseSI GKGCMC 1 cut(s) 502
BseXI GCAGC 4 cut(s) 134, 251, 289, 601
Bsh1236I CGCG 1 cut(s) 728
BsiHKAI GWGCWC 1 cut(s) 502
BsiHKCI CYCGRG 1 cut(s) 543
BsiSI CCGG 2 cut(s) 140, 521
BslFI GGGAC 2 cut(s) 142, 156
BslI CCNNNNNNNGG 1 cut(s) 395
BsmFI GGGAC 2 cut(s) 142, 156
BsoBI CYCGRG 1 cut(s) 543
Bsp1286I GDGCHC 1 cut(s) 502
Bsp1407I TGTACA 1 cut(s) 252
Bsp143I GATC 6 cut(s) 16, 61, 311, 550, 655, 688
BspACI CCGC 1 cut(s) 35
BspCNI CTCAG 2 cut(s) 574, 700
BspFNI CGCG 1 cut(s) 728
BspHI TCATGA 1 cut(s) 652
BspLI GGNNCC 1 cut(s) 351
BspPI GGATC 1 cut(s) 683
BspQI GCTCTTC 1 cut(s) 157
BspTI CTTAAG 1 cut(s) 878
BsrFI RCCGGY 1 cut(s) 520
BsrGI TGTACA 1 cut(s) 252
BsrI ACTGG 1 cut(s) 80
BssAI RCCGGY 1 cut(s) 520
BssMI GATC 6 cut(s) 16, 61, 311, 550, 655, 688
Bst4CI ACNGT 1 cut(s) 793
Bst6I CTCTTC 1 cut(s) 157
BstAFI CTTAAG 1 cut(s) 878
BstAUI TGTACA 1 cut(s) 252
BstBAI YACGTR 2 cut(s) 532, 814
BstC8I GCNNGC 1 cut(s) 526
BstDEI CTNAG 2 cut(s) 582, 708
BstFNI CGCG 1 cut(s) 728
BstHHI GCGC 1 cut(s) 728
BstKTI GATC 6 cut(s) 19, 64, 314, 553, 658, 691
BstMBI GATC 6 cut(s) 16, 61, 311, 550, 655, 688
BstMWI GCNNNNNNNGC 1 cut(s) 236
BstSCI CCNGG 1 cut(s) 139
BstSFI CTRYAG 2 cut(s) 10, 243
BstSLI GKGCMC 1 cut(s) 502
BstSNI TACGTA 1 cut(s) 532
BstUI CGCG 1 cut(s) 728
BstV1I GCAGC 4 cut(s) 134, 251, 289, 601
BsuI GTATCC 2 cut(s) 734, 779
BtsIMutI CAGTG 3 cut(s) 73, 565, 789
Cac8I GCNNGC 1 cut(s) 526
CciI TCATGA 1 cut(s) 652
CfoI GCGC 1 cut(s) 728
Cfr10I RCCGGY 1 cut(s) 520
Cfr13I GGNCC 2 cut(s) 349, 605
CseI GACGC 1 cut(s) 153
Csp6I GTAC 3 cut(s) 137, 253, 596
CviAII CATG 6 cut(s) 112, 233, 333, 382, 653, 750
CviJI RGCY 9 cut(s) 94, 191, 242, 528, 548, 586, 614, 832, 903
CviKI_1 RGCY 9 cut(s) 94, 191, 242, 528, 548, 586, 614, 832, 903
CviQI GTAC 3 cut(s) 137, 253, 596
DdeI CTNAG 2 cut(s) 582, 708
DpnI GATC 6 cut(s) 18, 63, 313, 552, 657, 690
DpnII GATC 6 cut(s) 16, 61, 311, 550, 655, 688
DraI TTTAAA 2 cut(s) 25, 807
Eam1104I CTCTTC 1 cut(s) 157
EarI CTCTTC 1 cut(s) 157
Eco105I TACGTA 1 cut(s) 532
Eco47I GGWCC 2 cut(s) 349, 605
Eco57I CTGAAG 1 cut(s) 603
Eco88I CYCGRG 1 cut(s) 543
EcoRI GAATTC 1 cut(s) 648
FaeI CATG 6 cut(s) 115, 236, 336, 385, 656, 753
FalI AAGNNNNNCTT 2 cut(s) 728, 760
FaqI GGGAC 2 cut(s) 142, 156
FatI CATG 6 cut(s) 111, 232, 332, 381, 652, 749
Fnu4HI GCNGC 4 cut(s) 148, 240, 303, 615
Fsp4HI GCNGC 4 cut(s) 148, 240, 303, 615
FspBI CTAG 1 cut(s) 483
GlaI GCGC 1 cut(s) 727
GluI GCNGC 4 cut(s) 148, 240, 303, 615
HapII CCGG 2 cut(s) 140, 521
HgaI GACGC 1 cut(s) 153
HhaI GCGC 1 cut(s) 728
Hin1II CATG 6 cut(s) 115, 236, 336, 385, 656, 753
Hin6I GCGC 1 cut(s) 726
HinP1I GCGC 1 cut(s) 726
HinfI GANTC 5 cut(s) 66, 130, 185, 281, 539
HpaII CCGG 2 cut(s) 140, 521
HphI GGTGA 2 cut(s) 71, 377
Hpy166II GTNNAC 3 cut(s) 81, 500, 608
Hpy188I TCNGA 2 cut(s) 280, 622
Hpy188III TCNNGA 3 cut(s) 543, 653, 783
Hpy8I GTNNAC 3 cut(s) 81, 500, 608
HpyAV CCTTC 2 cut(s) 230, 730
HpyCH4III ACNGT 1 cut(s) 793
HpyCH4IV ACGT 2 cut(s) 531, 813
HpyCH4V TGCA 5 cut(s) 174, 332, 500, 567, 749
HpyF10VI GCNNNNNNNGC 1 cut(s) 236
HpyF3I CTNAG 2 cut(s) 582, 708
HpySE526I ACGT 2 cut(s) 531, 813
Hsp92II CATG 6 cut(s) 115, 236, 336, 385, 656, 753
HspAI GCGC 1 cut(s) 726
Kzo9I GATC 6 cut(s) 16, 61, 311, 550, 655, 688
LguI GCTCTTC 1 cut(s) 157
LmnI GCTCC 1 cut(s) 236
LpnPI CCDG 5 cut(s) 61, 153, 534, 596, 768
Lsp1109I GCAGC 4 cut(s) 134, 251, 289, 601
LweI GCATC 2 cut(s) 183, 289
MaeI CTAG 1 cut(s) 483
MaeII ACGT 2 cut(s) 531, 813
MaeIII GTNAC 3 cut(s) 107, 365, 532
MalI GATC 6 cut(s) 18, 63, 313, 552, 657, 690
MboI GATC 6 cut(s) 16, 61, 311, 550, 655, 688
MboII GAAGA 3 cut(s) 144, 215, 895
MfeI CAATTG 1 cut(s) 390
MhlI GDGCHC 1 cut(s) 502
MlyI GAGTC 2 cut(s) 75, 124
MroXI GAANNNNTTC 1 cut(s) 890
MseI TTAA 6 cut(s) 24, 356, 588, 806, 854, 879
MspA1I CMGCKG 1 cut(s) 242
MspCI CTTAAG 1 cut(s) 878
MspI CCGG 2 cut(s) 140, 521
MspR9I CCNGG 1 cut(s) 141
MssI GTTTAAAC 1 cut(s) 807
MunI CAATTG 1 cut(s) 390
MvnI CGCG 1 cut(s) 728
MwoI GCNNNNNNNGC 1 cut(s) 236
NciI CCSGG 1 cut(s) 141
NdeII GATC 6 cut(s) 16, 61, 311, 550, 655, 688
NlaIII CATG 6 cut(s) 115, 236, 336, 385, 656, 753
NlaIV GGNNCC 1 cut(s) 351
NmuCI GTSAC 1 cut(s) 365
PaeR7I CTCGAG 1 cut(s) 543
PagI TCATGA 1 cut(s) 652
PciSI GCTCTTC 1 cut(s) 157
PdmI GAANNNNTTC 1 cut(s) 890
PfeI GAWTC 3 cut(s) 185, 281, 539
PflMI CCANNNNNTGG 1 cut(s) 395
PkrI GCNGC 4 cut(s) 149, 241, 304, 616
PleI GAGTC 2 cut(s) 74, 124
PmeI GTTTAAAC 1 cut(s) 807
PpsI GAGTC 2 cut(s) 74, 124
Ppu21I YACGTR 2 cut(s) 532, 814
PsiI TTATAA 3 cut(s) 48, 822, 916
PspN4I GGNNCC 1 cut(s) 351
PspPI GGNCC 2 cut(s) 349, 605
PvuII CAGCTG 1 cut(s) 242
RsaI GTAC 3 cut(s) 138, 254, 597
RsaNI GTAC 3 cut(s) 137, 253, 596
SapI GCTCTTC 1 cut(s) 157
SaqAI TTAA 6 cut(s) 24, 356, 588, 806, 854, 879
SatI GCNGC 4 cut(s) 148, 240, 303, 615
Sau3AI GATC 6 cut(s) 16, 61, 311, 550, 655, 688
Sau96I GGNCC 2 cut(s) 349, 605
ScaI AGTACT 1 cut(s) 597
SchI GAGTC 2 cut(s) 75, 124
ScrFI CCNGG 1 cut(s) 141
SduI GDGCHC 1 cut(s) 502
SfaNI GCATC 2 cut(s) 183, 289
SfcI CTRYAG 2 cut(s) 10, 243
Sfr274I CTCGAG 1 cut(s) 543
SinI GGWCC 2 cut(s) 349, 605
SlaI CTCGAG 1 cut(s) 543
SmlI CTYRAG 3 cut(s) 132, 543, 878
SmoI CTYRAG 3 cut(s) 132, 543, 878
SnaBI TACGTA 1 cut(s) 532
SsiI CCGC 1 cut(s) 35
SspMI CTAG 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 139
TaaI ACNGT 1 cut(s) 793
TaiI ACGT 2 cut(s) 534, 816
TaqI TCGA 2 cut(s) 64, 544
TatI WGTACW 2 cut(s) 252, 595
TfiI GAWTC 3 cut(s) 185, 281, 539
Tru1I TTAA 6 cut(s) 24, 356, 588, 806, 854, 879
Tru9I TTAA 6 cut(s) 24, 356, 588, 806, 854, 879
TscAI CASTG 3 cut(s) 80, 565, 796
TseFI GTSAC 1 cut(s) 365
TseI GCWGC 4 cut(s) 147, 239, 302, 614
Tsp45I GTSAC 1 cut(s) 365
TspDTI ATGAA 2 cut(s) 641, 900
TspGWI ACGGA 2 cut(s) 98, 459
TspRI CASTG 3 cut(s) 80, 565, 796
Van91I CCANNNNNTGG 1 cut(s) 395
Vha464I CTTAAG 1 cut(s) 878
VneI GTGCAC 1 cut(s) 498
VpaK11BI GGWCC 2 cut(s) 349, 605
XapI RAATTY 5 cut(s) 53, 413, 648, 871, 890
XhoI CTCGAG 1 cut(s) 543
XmnI GAANNNNTTC 1 cut(s) 890
XspI CTAG 1 cut(s) 483
ZrmI AGTACT 1 cut(s) 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.