Belongs to the glycosyl hydrolase 1 family
Download the Full Tree (.nwk) file, then upload it to any of the following tools to customize colors, fonts, annotations, clades, and branch support.
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AccB1I | GGYRCC | 2 cut(s) | 250, 888 |
| AclI | AACGTT | 1 cut(s) | 919 |
| AclWI | GGATC | 4 cut(s) | 126, 200, 626, 671 |
| AcsI | RAATTY | 5 cut(s) | 167, 298, 537, 591, 826 |
| AcuI | CTGAAG | 1 cut(s) | 546 |
| AfaI | GTAC | 1 cut(s) | 307 |
| AgeI | ACCGGT | 1 cut(s) | 290 |
| AgsI | TTSAA | 4 cut(s) | 673, 755, 821, 916 |
| AjnI | CCWGG | 1 cut(s) | 253 |
| AjuI | GAANNNNNNNTTGG | 2 cut(s) | 544, 576 |
| AluBI | AGCT | 7 cut(s) | 16, 154, 337, 353, 479, 936, 953 |
| AluI | AGCT | 7 cut(s) | 16, 154, 337, 353, 479, 936, 953 |
| Alw21I | GWGCWC | 2 cut(s) | 456, 481 |
| AlwI | GGATC | 4 cut(s) | 126, 200, 626, 671 |
| ApeKI | GCWGC | 5 cut(s) | 353, 373, 467, 557, 1014 |
| ApoI | RAATTY | 5 cut(s) | 167, 298, 537, 591, 826 |
| AsiGI | ACCGGT | 1 cut(s) | 290 |
| AspS9I | GGNCC | 3 cut(s) | 508, 548, 961 |
| AsuHPI | GGTGA | 1 cut(s) | 185 |
| AvaII | GGWCC | 3 cut(s) | 508, 548, 961 |
| BanI | GGYRCC | 2 cut(s) | 250, 888 |
| BanII | GRGCYC | 1 cut(s) | 481 |
| BbsI | GAAGAC | 1 cut(s) | 623 |
| Bbv12I | GWGCWC | 2 cut(s) | 456, 481 |
| BbvI | GCAGC | 5 cut(s) | 340, 385, 454, 544, 1026 |
| BccI | CCATC | 3 cut(s) | 2, 492, 764 |
| BcgI | CGANNNNNNTGC | 1 cut(s) | 1006 |
| BciT130I | CCWGG | 1 cut(s) | 255 |
| BciVI | GTATCC | 2 cut(s) | 709, 782 |
| BfaI | CTAG | 3 cut(s) | 416, 482, 809 |
| BfuI | GTATCC | 2 cut(s) | 709, 782 |
| BglII | AGATCT | 1 cut(s) | 598 |
| BisI | GCNGC | 5 cut(s) | 354, 374, 468, 558, 1015 |
| BlsI | GCNGC | 5 cut(s) | 355, 375, 469, 559, 1016 |
| Bme1390I | CCNGG | 1 cut(s) | 255 |
| Bme18I | GGWCC | 3 cut(s) | 508, 548, 961 |
| BmgT120I | GGNCC | 3 cut(s) | 508, 548, 961 |
| BmiI | GGNNCC | 3 cut(s) | 252, 510, 890 |
| BmrFI | CCNGG | 1 cut(s) | 255 |
| BmrI | ACTGGG | 2 cut(s) | 30, 124 |
| BmuI | ACTGGG | 2 cut(s) | 30, 124 |
| BpiI | GAAGAC | 1 cut(s) | 623 |
| BsaWI | WCCGGW | 1 cut(s) | 290 |
| Bse118I | RCCGGY | 2 cut(s) | 290, 436 |
| Bse1I | ACTGG | 5 cut(s) | 25, 119, 194, 431, 914 |
| Bse3DI | GCAATG | 2 cut(s) | 88, 803 |
| BseBI | CCWGG | 1 cut(s) | 255 |
| BseGI | GGATG | 2 cut(s) | 503, 775 |
| BseMI | GCAATG | 2 cut(s) | 88, 803 |
| BseMII | CTCAG | 2 cut(s) | 435, 642 |
| BseNI | ACTGG | 5 cut(s) | 25, 119, 194, 431, 914 |
| BseXI | GCAGC | 5 cut(s) | 340, 385, 454, 544, 1026 |
| BsgI | GTGCAG | 2 cut(s) | 453, 888 |
| BshNI | GGYRCC | 2 cut(s) | 250, 888 |
| BshTI | ACCGGT | 1 cut(s) | 290 |
| BsiHKAI | GWGCWC | 2 cut(s) | 456, 481 |
| BsiSI | CCGG | 2 cut(s) | 291, 437 |
| Bsp1286I | GDGCHC | 2 cut(s) | 456, 481 |
| Bsp143I | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| BspCNI | CTCAG | 2 cut(s) | 436, 643 |
| BspLI | GGNNCC | 3 cut(s) | 252, 510, 890 |
| BspPI | GGATC | 4 cut(s) | 126, 200, 626, 671 |
| BspT107I | GGYRCC | 2 cut(s) | 250, 888 |
| BsrDI | GCAATG | 2 cut(s) | 88, 803 |
| BsrFI | RCCGGY | 2 cut(s) | 290, 436 |
| BsrI | ACTGG | 5 cut(s) | 25, 119, 194, 431, 914 |
| BssAI | RCCGGY | 2 cut(s) | 290, 436 |
| BssMI | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| Bst2UI | CCWGG | 1 cut(s) | 255 |
| Bst4CI | ACNGT | 1 cut(s) | 841 |
| Bst6I | CTCTTC | 2 cut(s) | 113, 971 |
| BstC8I | GCNNGC | 2 cut(s) | 152, 1019 |
| BstDEI | CTNAG | 3 cut(s) | 444, 473, 651 |
| BstF5I | GGATG | 2 cut(s) | 503, 775 |
| BstKTI | GATC | 5 cut(s) | 121, 195, 601, 634, 679 |
| BstMBI | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| BstMWI | GCNNNNNNNGC | 5 cut(s) | 248, 350, 476, 886, 942 |
| BstNI | CCWGG | 1 cut(s) | 255 |
| BstNSI | RCATGY | 1 cut(s) | 1021 |
| BstSCI | CCNGG | 1 cut(s) | 253 |
| BstV1I | GCAGC | 5 cut(s) | 340, 385, 454, 544, 1026 |
| BstV2I | GAAGAC | 1 cut(s) | 623 |
| BstX2I | RGATCY | 2 cut(s) | 118, 598 |
| BstYI | RGATCY | 2 cut(s) | 118, 598 |
| BsuI | GTATCC | 2 cut(s) | 709, 782 |
| BtsCI | GGATG | 2 cut(s) | 503, 775 |
| BtsI | GCAGTG | 1 cut(s) | 243 |
| BtsIMutI | CAGTG | 5 cut(s) | 112, 187, 243, 837, 907 |
| Cac8I | GCNNGC | 2 cut(s) | 152, 1019 |
| Cfr10I | RCCGGY | 2 cut(s) | 290, 436 |
| Cfr13I | GGNCC | 3 cut(s) | 508, 548, 961 |
| CseI | GACGC | 1 cut(s) | 267 |
| Csp6I | GTAC | 1 cut(s) | 306 |
| CspAI | ACCGGT | 1 cut(s) | 290 |
| CspCI | CAANNNNNGTGG | 2 cut(s) | 728, 763 |
| CviAII | CATG | 2 cut(s) | 798, 1018 |
| CviQI | GTAC | 1 cut(s) | 306 |
| DdeI | CTNAG | 3 cut(s) | 444, 473, 651 |
| DpnI | GATC | 5 cut(s) | 120, 194, 600, 633, 678 |
| DpnII | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| Eam1104I | CTCTTC | 2 cut(s) | 113, 971 |
| EarI | CTCTTC | 2 cut(s) | 113, 971 |
| Ecl136II | GAGCTC | 1 cut(s) | 479 |
| Eco24I | GRGCYC | 1 cut(s) | 481 |
| Eco47I | GGWCC | 3 cut(s) | 508, 548, 961 |
| Eco53kI | GAGCTC | 1 cut(s) | 479 |
| Eco57I | CTGAAG | 1 cut(s) | 546 |
| EcoICRI | GAGCTC | 1 cut(s) | 479 |
| EcoRI | GAATTC | 4 cut(s) | 298, 537, 591, 826 |
| EcoRII | CCWGG | 1 cut(s) | 253 |
| EcoT38I | GRGCYC | 1 cut(s) | 481 |
| FaeI | CATG | 2 cut(s) | 801, 1021 |
| FalI | AAGNNNNNCTT | 4 cut(s) | 687, 719, 776, 808 |
| FatI | CATG | 2 cut(s) | 797, 1017 |
| Fnu4HI | GCNGC | 5 cut(s) | 354, 374, 468, 558, 1015 |
| FokI | GGATG | 2 cut(s) | 510, 782 |
| FriOI | GRGCYC | 1 cut(s) | 481 |
| Fsp4HI | GCNGC | 5 cut(s) | 354, 374, 468, 558, 1015 |
| FspBI | CTAG | 3 cut(s) | 416, 482, 809 |
| GluI | GCNGC | 5 cut(s) | 354, 374, 468, 558, 1015 |
| HapII | CCGG | 2 cut(s) | 291, 437 |
| HgaI | GACGC | 1 cut(s) | 267 |
| Hin1II | CATG | 2 cut(s) | 801, 1021 |
| HincII | GTYRAC | 1 cut(s) | 724 |
| HindII | GTYRAC | 1 cut(s) | 724 |
| HindIII | AAGCTT | 1 cut(s) | 934 |
| HinfI | GANTC | 1 cut(s) | 42 |
| HpaII | CCGG | 2 cut(s) | 291, 437 |
| HphI | GGTGA | 1 cut(s) | 185 |
| Hpy166II | GTNNAC | 2 cut(s) | 31, 724 |
| Hpy188I | TCNGA | 4 cut(s) | 268, 445, 565, 845 |
| Hpy188III | TCNNGA | 3 cut(s) | 482, 596, 964 |
| Hpy8I | GTNNAC | 2 cut(s) | 31, 724 |
| HpyAV | CCTTC | 2 cut(s) | 32, 761 |
| HpyCH4III | ACNGT | 1 cut(s) | 841 |
| HpyCH4IV | ACGT | 3 cut(s) | 576, 645, 919 |
| HpyCH4V | TGCA | 9 cut(s) | 4, 236, 288, 356, 470, 797, 905, 1014, 1021 |
| HpyF10VI | GCNNNNNNNGC | 5 cut(s) | 248, 350, 476, 886, 942 |
| HpyF3I | CTNAG | 3 cut(s) | 444, 473, 651 |
| HpySE526I | ACGT | 3 cut(s) | 576, 645, 919 |
| Hsp92II | CATG | 2 cut(s) | 801, 1021 |
| Kzo9I | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| LmnI | GCTCC | 1 cut(s) | 342 |
| Lsp1109I | GCAGC | 5 cut(s) | 340, 385, 454, 544, 1026 |
| MaeI | CTAG | 3 cut(s) | 416, 482, 809 |
| MaeII | ACGT | 3 cut(s) | 576, 645, 919 |
| MaeIII | GTNAC | 4 cut(s) | 100, 158, 646, 833 |
| MalI | GATC | 5 cut(s) | 120, 194, 600, 633, 678 |
| MboI | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| MboII | GAAGA | 6 cut(s) | 100, 113, 149, 628, 988, 1016 |
| MflI | RGATCY | 2 cut(s) | 118, 598 |
| MhlI | GDGCHC | 2 cut(s) | 456, 481 |
| MluCI | AATT | 8 cut(s) | 167, 243, 283, 298, 537, 591, 683, 826 |
| MlyI | GAGTC | 1 cut(s) | 51 |
| MnlI | CCTC | 7 cut(s) | 116, 439, 581, 752, 934, 935, 952 |
| MseI | TTAA | 5 cut(s) | 201, 531, 686, 705, 783 |
| MslI | CAYNNNNRTG | 1 cut(s) | 462 |
| MspA1I | CMGCKG | 1 cut(s) | 353 |
| MspI | CCGG | 2 cut(s) | 291, 437 |
| MspR9I | CCNGG | 1 cut(s) | 255 |
| MvaI | CCWGG | 1 cut(s) | 255 |
| MwoI | GCNNNNNNNGC | 5 cut(s) | 248, 350, 476, 886, 942 |
| NdeII | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| NlaIII | CATG | 2 cut(s) | 801, 1021 |
| NlaIV | GGNNCC | 3 cut(s) | 252, 510, 890 |
| NspI | RCATGY | 1 cut(s) | 1021 |
| PaeI | GCATGC | 1 cut(s) | 1021 |
| PflFI | GACNNNGTC | 1 cut(s) | 959 |
| PinAI | ACCGGT | 1 cut(s) | 290 |
| PkrI | GCNGC | 5 cut(s) | 355, 375, 469, 559, 1016 |
| PleI | GAGTC | 1 cut(s) | 50 |
| PpsI | GAGTC | 1 cut(s) | 50 |
| Psp124BI | GAGCTC | 1 cut(s) | 481 |
| Psp1406I | AACGTT | 1 cut(s) | 919 |
| Psp6I | CCWGG | 1 cut(s) | 253 |
| PspGI | CCWGG | 1 cut(s) | 253 |
| PspN4I | GGNNCC | 3 cut(s) | 252, 510, 890 |
| PspPI | GGNCC | 3 cut(s) | 508, 548, 961 |
| PsuI | RGATCY | 2 cut(s) | 118, 598 |
| PsyI | GACNNNGTC | 1 cut(s) | 959 |
| PvuII | CAGCTG | 1 cut(s) | 353 |
| RsaI | GTAC | 1 cut(s) | 307 |
| RsaNI | GTAC | 1 cut(s) | 306 |
| RseI | CAYNNNNRTG | 1 cut(s) | 462 |
| SacI | GAGCTC | 1 cut(s) | 481 |
| SaqAI | TTAA | 5 cut(s) | 201, 531, 686, 705, 783 |
| SatI | GCNGC | 5 cut(s) | 354, 374, 468, 558, 1015 |
| Sau3AI | GATC | 5 cut(s) | 118, 192, 598, 631, 676 |
| Sau96I | GGNCC | 3 cut(s) | 508, 548, 961 |
| SchI | GAGTC | 1 cut(s) | 51 |
| ScrFI | CCNGG | 1 cut(s) | 255 |
| SduI | GDGCHC | 2 cut(s) | 456, 481 |
| SgrAI | CRCCGGYG | 1 cut(s) | 290 |
| SinI | GGWCC | 3 cut(s) | 508, 548, 961 |
| SmiMI | CAYNNNNRTG | 1 cut(s) | 462 |
| SphI | GCATGC | 1 cut(s) | 1021 |
| Sse9I | AATT | 8 cut(s) | 167, 243, 283, 298, 537, 591, 683, 826 |
| SspI | AATATT | 1 cut(s) | 1010 |
| SspMI | CTAG | 3 cut(s) | 416, 482, 809 |
| SstI | GAGCTC | 1 cut(s) | 481 |
| StyD4I | CCNGG | 1 cut(s) | 253 |
| TaaI | ACNGT | 1 cut(s) | 841 |
| TaiI | ACGT | 3 cut(s) | 579, 648, 922 |
| TaqI | TCGA | 3 cut(s) | 595, 693, 1026 |
| TasI | AATT | 8 cut(s) | 167, 243, 283, 298, 537, 591, 683, 826 |
| Tru1I | TTAA | 5 cut(s) | 201, 531, 686, 705, 783 |
| Tru9I | TTAA | 5 cut(s) | 201, 531, 686, 705, 783 |
| TscAI | CASTG | 5 cut(s) | 119, 194, 243, 844, 914 |
| TseI | GCWGC | 5 cut(s) | 353, 373, 467, 557, 1014 |
| TspDTI | ATGAA | 3 cut(s) | 84, 156, 989 |
| TspRI | CASTG | 5 cut(s) | 119, 194, 243, 844, 914 |
| Tth111I | GACNNNGTC | 1 cut(s) | 959 |
| VpaK11BI | GGWCC | 3 cut(s) | 508, 548, 961 |
| XapI | RAATTY | 5 cut(s) | 167, 298, 537, 591, 826 |
| XbaI | TCTAGA | 1 cut(s) | 481 |
| XceI | RCATGY | 1 cut(s) | 1021 |
| XspI | CTAG | 3 cut(s) | 416, 482, 809 |