FvH4_7g07770

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb7
OG0000294
Physical Location & Seq
Forward (+)
7668050 .. 7672899
4850 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_7g07770.t1

Sequence Viewer

Length: 2670 bp
ATGGAGTTTCAGGGGTTGAGGCAAACAGTGATCATGGCATTTGTGACCTTCTGTTGCCTCAGTGGCCACTTGTGTGCTGATATTGAGAAAACAAATAATGAAACTAATCCCATTGTTGTTGAAGATCAGGTAAAGGTCAATGTCGGAGTGATTCTCGACATGGGATCAAGGGAAGGAAAGATTATTCTCAGCTGTATATCAATGGCCCTCTCTGATTTTTACCATCTGCATGATAACTACAGCACAAGAGTAGTTCTCCACAGTAAGGATTCCAAAGGTGAACCTCTGCCTGCACTGTCAGCTGCCATTGGTCTTTTGGAAACCATCAGAGTGGAATCAATAATTGGTGCACAAACAAGAGAGGAGGCAAACCTTTTAGCAGAATTAGGAGAAGCGGCTAAAGTCCCTGTGATGTCTCTTTCTCCTCCTCTGGCTGACAATAAATACCGTTTCTTTGTTGATATCACGCGCAGTGATCAAGCCGCTGAAGTTAGGGGAATCAGTGCCCTTATTGAAGTTTTCAGATGGAGGGATGTTATACTTTTATATGATAATAAAGCATATGAGGAAGATTTCATTCCATCATTAGTCAACTCTTTCCAGGAGATCACACATGGGACTGTTTCATTCAAGAGTTCCAATATTGCTTCCTCCTCATCAAATGAAGAAATCATTGAAGAGCTCCAAAAGCTGACGAGATTGAAGATTAAGGTATTTGTGGTGCATGTGTCACATTTGTTTGCGCCGCGCCTTTTCTTAAATGCTAATAAGTTAGGGATGATGAGTGAAGGGTATGTCTGGATCGTGACTTCGAATAGCATGAACTTATTGCAGTCCATGAATTTATCTGTTATTGAGTCGATGCAAGGAGTGGTTGGTTTCAAGTCTTCTATTCCAGCATCGATGAGCCTTCACAGTCTTGCTTCAAGATTAAGGAGGAAGTTTTACTTGGAGGATCCCAATATGGAAGCGATAAGGGAGTTGAATGCAGAAGCAGTCTGGGCATATGATGCAACTTGGGCTCTAGCAGAAGCTGCTGAAAAAGCAAGGCTTACATATTCTAGCACCAGATCCTCCAAAAATGGAGTTTTGCTTCTTAGTGGGATATTGCAGACTAGATTTAGAGGTTTAAGCAGTGATATTCAGTATCTAAATGGAAAATTGATTCCATCAGAACCATTTGAGATAGTTAATGTTATTGGAAAAGGTGAGATCAAAAGGATTGGTTTTTGGCCTTGCAAAGAAGAAGTAAAATCCGGAAAAGGGTCGCCCCATCAGCAGCTTATTCATAGGAGGAGTAATTTAGTTTCTACAATTGATCTTGAAAGAATCATATGGCCTGGAGGATCCATGAAGGAATTGAGTAGTAGTGAAACAATTAAACTAAGAATTGGTGTTCCAGTGAGATTTGGGTTCAAGGAACTTGTCCGTGTCGAGCATGATCTTGAAACCAATACGACTCATGTCACTGGCTTCTCTATAGATGTATTCAAAGCTGCAATAGAAGCTTTGCCGTATGAAGTGGTTTATGAGTTTATTCCATTTGAGGATGCCAATGGAAATGCTGCTGGGACTTATAATGATCTTGTCTACCAGGTTTATCTCAAGAACTTCGATGCTGTTGTTGGAGATGTTACGATTACAGAGAACAGATCTCAATATGTTGATTTTACAATACCATATACGGACTTAGGTGTGGGAATGTTGGTACCAAATCAAGATAACTTGTGGATTTTCTTTAAACCACTATCAAAATATCTTTGGATAACAAGTGCAGGCTTCTTCATCCTAACCGGTTTTGTTATCTGGATAATTGAGCATCCTAATAACGAAGACTTCCAAGGCACAGCAGGGAAACAAATTGGGACTGTATTCTGGTTCTCCTTCTCTACTCTTGTGTTCGCATATACGGAGAAGTTGTCAAATAATTTGGCAAAGTTTGTAGTGATCATATGGGTGTTTGTCGTGCTTATATTGACGTCGACTTACACTGCAACTTTAGCATCAATGATGACTGTCAAACAGATACAGTTAAACTCAAGAGGAAACTCTATAGGTTACCAATCGGGTTCCTTAGGAGTTATATTGAACTTGAATTTCAAAGGCCTTAAGCCATATCGTTCAAGTGAGGAGTATCTTGAAGCTTTATCAAAAGGAACCAAGCATAATGGTGTTTCTGGGATAATTGACGAGGTCCCATACATCAAAATCTTCCTCGCAAACAATTCTGCTGACTACTCGATGATTAAAACCGAGTCTATCACCAATGGTTTTGCCTTTGTTTTCCCTAAAGGTTCGAAATTGGTTCAGGATGTGTCGAGGCAAATCGAACGTCTGAGACAAGAAGAGAAGCTTATAGAGATGGAGAAGACCTGGTTTCTTAGAAAAACAACTCTTATGTCTGATGAGGATATTAAAGATCCCAATACGATTGACCTCCACGACTTTCGCGGTCTATTTCTTATTAGTGGTGCTTCTTTAGCTCTTGCTCTTTTCTTGTTCATAGTTGTATCTGATACATTTAGAAATCTGATTAGAGGACTAGTGCAGCTGATTCGAAGACAACTGCAGCGTTTAAGGGTGTTTGTCTCGAACACAGCATGCAGTAGAACTGAACAGAACTGCTGTATGTCACTTCCAATGCAGCTGTACATATATACTGTCCGTTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

890

Amino Acids

100.01

Weight (kDa)

6.27

Isoelectric Point (pI)

37.45

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 46 - 350 3.9e-07 Periplasmic binding protein
ANF_receptor PF01094 65 - 400 2.6e-53 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 480 - 572 7.7e-11 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 485 - 794 4.2e-13 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 586 - 825 1.1e-26 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1578
AatII GACGTC 1 cut(s) 1982
Acc65I GGTACC 1 cut(s) 1708
AccB1I GGYRCC 1 cut(s) 1708
AccI GTMKAC 2 cut(s) 1590, 1982
AccII CGCG 3 cut(s) 469, 748, 2451
AccIII TCCGGA 1 cut(s) 1256
AciI CCGC 4 cut(s) 395, 483, 746, 2451
AclWI GGATC 8 cut(s) 172, 809, 950, 963, 1065, 1341, 1354, 2414
AcoI YGGCCR 1 cut(s) 64
AcsI RAATTY 2 cut(s) 841, 2095
AcuI CTGAAG 1 cut(s) 507
AcyI GRCGYC 1 cut(s) 1979
AfaI GTAC 2 cut(s) 1710, 2651
AfiI CCNNNNNNNGG 2 cut(s) 265, 1263
AflII CTTAAG 1 cut(s) 2108
AgeI ACCGGT 1 cut(s) 1793
AhlI ACTAGT 1 cut(s) 2542
AjnI CCWGG 4 cut(s) 600, 1339, 1593, 2372
AjuI GAANNNNNNNTTGG 4 cut(s) 327, 359, 932, 964
AleI CACNNNNGTG 1 cut(s) 72
Alw21I GWGCWC 2 cut(s) 352, 684
Alw26I GTCTC 3 cut(s) 420, 2332, 2593
Alw44I GTGCAC 1 cut(s) 348
AlwI GGATC 8 cut(s) 172, 809, 950, 963, 1065, 1341, 1354, 2414
AlwNI CAGNNNCTG 1 cut(s) 1034
Aor13HI TCCGGA 1 cut(s) 1256
AoxI GGCC 5 cut(s) 64, 204, 1232, 1337, 2104
ApaLI GTGCAC 1 cut(s) 348
ApeKI GCWGC 8 cut(s) 302, 1034, 1279, 1496, 1565, 2548, 2569, 2644
ApoI RAATTY 2 cut(s) 841, 2095
ArsI GACNNNNNNTTYG 2 cut(s) 2317, 2349
AsiGI ACCGGT 1 cut(s) 1793
Asp718I GGTACC 1 cut(s) 1708
AspLEI GCGC 3 cut(s) 471, 745, 750
AspS9I GGNCC 2 cut(s) 205, 2194
AsuHPI GGTGA 3 cut(s) 290, 1220, 2254
AsuII TTCGAA 3 cut(s) 812, 2297, 2557
AvaII GGWCC 1 cut(s) 2194
AxyI CCTNAGG 1 cut(s) 2074
BaeGI GKGCMC 2 cut(s) 352, 508
BalI TGGCCA 1 cut(s) 66
BamHI GGATCC 2 cut(s) 955, 1346
BanI GGYRCC 1 cut(s) 1708
BanII GRGCYC 2 cut(s) 684, 1024
BbsI GAAGAC 4 cut(s) 879, 1839, 2375, 2566
Bbv12I GWGCWC 2 cut(s) 352, 684
BbvI GCAGC 8 cut(s) 289, 1021, 1291, 1483, 1552, 2560, 2581, 2656
BccI CCATC 7 cut(s) 231, 332, 519, 589, 1177, 1281, 2356
BceAI ACGGC 1 cut(s) 1498
BcgI CGANNNNNNTGC 2 cut(s) 2537, 2571
BciT130I CCWGG 4 cut(s) 602, 1341, 1595, 2374
BclI TGATCA 3 cut(s) 30, 475, 1947
BcoDI GTCTC 3 cut(s) 420, 2332, 2593
BcuI ACTAGT 1 cut(s) 2542
BfaI CTAG 4 cut(s) 1025, 1062, 1116, 2543
BfmI CTRYAG 4 cut(s) 238, 1479, 2052, 2567
BfrI CTTAAG 1 cut(s) 2108
BglI GCCNNNNNGGC 1 cut(s) 63
BglII AGATCT 1 cut(s) 1652
Bme1390I CCNGG 4 cut(s) 602, 1341, 1595, 2374
Bme18I GGWCC 1 cut(s) 2194
BmgT120I GGNCC 2 cut(s) 205, 2194
BmiI GGNNCC 6 cut(s) 957, 1348, 1710, 2071, 2158, 2196
BmrFI CCNGG 4 cut(s) 602, 1341, 1595, 2374
BmsI GCATC 7 cut(s) 852, 908, 1000, 1540, 1606, 1828, 2012
BoxI GACNNNNGTC 1 cut(s) 1463
BpiI GAAGAC 4 cut(s) 879, 1839, 2375, 2566
BplI GAGNNNNNCTC 4 cut(s) 138, 170, 240, 272
BpmI CTGGAG 1 cut(s) 1362
Bpu14I TTCGAA 3 cut(s) 812, 2297, 2557
BpuEI CTTGAG 2 cut(s) 1589, 2023
Bsa29I ATCGAT 1 cut(s) 902
BsaHI GRCGYC 1 cut(s) 1979
BsaJI CCNNGG 1 cut(s) 1840
BsaWI WCCGGW 2 cut(s) 1256, 1793
BsaXI ACNNNNNCTCC 2 cut(s) 1904, 1934
Bsc4I CCNNNNNNNGG 2 cut(s) 265, 1263
Bse118I RCCGGY 1 cut(s) 1793
Bse1I ACTGG 2 cut(s) 1400, 1474
Bse21I CCTNAGG 1 cut(s) 2074
BseAI TCCGGA 1 cut(s) 1256
BseBI CCWGG 4 cut(s) 602, 1341, 1595, 2374
BseCI ATCGAT 1 cut(s) 902
BseDI CCNNGG 1 cut(s) 1840
BseGI GGATG 6 cut(s) 538, 783, 1555, 1785, 1819, 2317
BseLI CCNNNNNNNGG 2 cut(s) 265, 1263
BseMII CTCAG 3 cut(s) 73, 202, 2327
BseNI ACTGG 2 cut(s) 1400, 1474
BseRI GAGGAG 6 cut(s) 377, 414, 417, 643, 1309, 2144
BseSI GKGCMC 2 cut(s) 352, 508
BseXI GCAGC 8 cut(s) 289, 1021, 1291, 1483, 1552, 2560, 2581, 2656
BseYI CCCAGC 1 cut(s) 1568
BsgI GTGCAG 3 cut(s) 276, 1794, 2567
Bsh1236I CGCG 3 cut(s) 469, 748, 2451
BshFI GGCC 5 cut(s) 66, 206, 1234, 1339, 2106
BshNI GGYRCC 1 cut(s) 1708
BshTI ACCGGT 1 cut(s) 1793
BshVI ATCGAT 1 cut(s) 902
BsiHKAI GWGCWC 2 cut(s) 352, 684
BsiSI CCGG 2 cut(s) 1257, 1794
BslFI GGGAC 5 cut(s) 389, 631, 1585, 1879, 2180
BslI CCNNNNNNNGG 2 cut(s) 265, 1263
BsmAI GTCTC 3 cut(s) 420, 2332, 2593
BsmFI GGGAC 5 cut(s) 389, 631, 1585, 1879, 2180
BsmI GAATGC 1 cut(s) 991
BsnI GGCC 5 cut(s) 66, 206, 1234, 1339, 2106
Bsp119I TTCGAA 3 cut(s) 812, 2297, 2557
Bsp1286I GDGCHC 4 cut(s) 352, 508, 684, 1024
Bsp13I TCCGGA 1 cut(s) 1256
Bsp1407I TGTACA 1 cut(s) 2649
BspACI CCGC 4 cut(s) 395, 483, 746, 2451
BspANI GGCC 5 cut(s) 66, 206, 1234, 1339, 2106
BspCNI CTCAG 3 cut(s) 72, 201, 2328
BspDI ATCGAT 1 cut(s) 902
BspEI TCCGGA 1 cut(s) 1256
BspFNI CGCG 3 cut(s) 469, 748, 2451
BspLI GGNNCC 6 cut(s) 957, 1348, 1710, 2071, 2158, 2196
BspMAI CTGCAG 1 cut(s) 2571
BspPI GGATC 8 cut(s) 172, 809, 950, 963, 1065, 1341, 1354, 2414
BspQI GCTCTTC 1 cut(s) 672
BspT104I TTCGAA 3 cut(s) 812, 2297, 2557
BspT107I GGYRCC 1 cut(s) 1708
BspTI CTTAAG 1 cut(s) 2108
BsrFI RCCGGY 1 cut(s) 1793
BsrGI TGTACA 1 cut(s) 2649
BsrI ACTGG 2 cut(s) 1400, 1474
BssAI RCCGGY 1 cut(s) 1793
BssECI CCNNGG 1 cut(s) 1840
BssNI GRCGYC 1 cut(s) 1979
BssT1I CCWWGG 1 cut(s) 1840
Bst2UI CCWGG 4 cut(s) 602, 1341, 1595, 2374
Bst6I CTCTTC 2 cut(s) 672, 2340
BstACI GRCGYC 1 cut(s) 1979
BstAFI CTTAAG 1 cut(s) 2108
BstAPI GCANNNNNTGC 2 cut(s) 1010, 1034
BstAUI TGTACA 1 cut(s) 2649
BstBI TTCGAA 3 cut(s) 812, 2297, 2557
BstC8I GCNNGC 3 cut(s) 291, 1777, 2602
BstDEI CTNAG 8 cut(s) 59, 188, 1097, 1385, 1690, 2074, 2336, 2381
BstEII GGTNACC 1 cut(s) 2057
BstF5I GGATG 6 cut(s) 538, 783, 1555, 1785, 1819, 2317
BstFNI CGCG 3 cut(s) 469, 748, 2451
BstHHI GCGC 3 cut(s) 471, 745, 750
BstMAI GTCTC 3 cut(s) 420, 2332, 2593
BstNI CCWGG 4 cut(s) 602, 1341, 1595, 2374
BstNSI RCATGY 2 cut(s) 728, 2604
BstPAI GACNNNNGTC 1 cut(s) 1463
BstPI GGTNACC 1 cut(s) 2057
BstSCI CCNGG 4 cut(s) 600, 1339, 1593, 2372
BstSFI CTRYAG 4 cut(s) 238, 1479, 2052, 2567
BstSLI GKGCMC 2 cut(s) 352, 508
BstUI CGCG 3 cut(s) 469, 748, 2451
BstV1I GCAGC 8 cut(s) 289, 1021, 1291, 1483, 1552, 2560, 2581, 2656
BstV2I GAAGAC 4 cut(s) 879, 1839, 2375, 2566
BstX2I RGATCY 5 cut(s) 955, 1070, 1346, 1652, 2419
BstXI CCANNNNNNTGG 1 cut(s) 331
BstYI RGATCY 5 cut(s) 955, 1070, 1346, 1652, 2419
Bsu15I ATCGAT 1 cut(s) 902
Bsu36I CCTNAGG 1 cut(s) 2074
BsuRI GGCC 5 cut(s) 66, 206, 1234, 1339, 2106
BsuTUI ATCGAT 1 cut(s) 902
BtsCI GGATG 6 cut(s) 538, 783, 1555, 1785, 1819, 2317
BtsI GCAGTG 3 cut(s) 478, 1141, 1989
BtsIMutI CAGTG 9 cut(s) 33, 67, 293, 478, 508, 1141, 1407, 1467, 1989
Cac8I GCNNGC 3 cut(s) 291, 1777, 2602
CaiI CAGNNNCTG 1 cut(s) 1034
CfoI GCGC 3 cut(s) 471, 745, 750
Cfr10I RCCGGY 1 cut(s) 1793
Cfr13I GGNCC 2 cut(s) 205, 2194
ClaI ATCGAT 1 cut(s) 902
CsiI ACCWGGT 2 cut(s) 1593, 2372
Csp6I GTAC 2 cut(s) 1709, 2650
CspAI ACCGGT 1 cut(s) 1793
CviQI GTAC 2 cut(s) 1709, 2650
DdeI CTNAG 8 cut(s) 59, 188, 1097, 1385, 1690, 2074, 2336, 2381
DraI TTTAAA 1 cut(s) 1741
EaeI YGGCCR 1 cut(s) 64
Eam1104I CTCTTC 2 cut(s) 672, 2340
EarI CTCTTC 2 cut(s) 672, 2340
Ecl136II GAGCTC 1 cut(s) 682
Eco130I CCWWGG 1 cut(s) 1840
Eco147I AGGCCT 1 cut(s) 2106
Eco24I GRGCYC 2 cut(s) 684, 1024
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 1 cut(s) 2194
Eco53kI GAGCTC 1 cut(s) 682
Eco57I CTGAAG 1 cut(s) 507
Eco81I CCTNAGG 1 cut(s) 2074
Eco91I GGTNACC 1 cut(s) 2057
EcoICRI GAGCTC 1 cut(s) 682
EcoO109I RGGNCCY 1 cut(s) 2194
EcoO65I GGTNACC 1 cut(s) 2057
EcoRII CCWGG 4 cut(s) 600, 1339, 1593, 2372
EcoRV GATATC 1 cut(s) 463
EcoT14I CCWWGG 1 cut(s) 1840
EcoT38I GRGCYC 2 cut(s) 684, 1024
ErhI CCWWGG 1 cut(s) 1840
FalI AAGNNNNNCTT 6 cut(s) 932, 964, 1035, 1067, 2337, 2369
FaqI GGGAC 5 cut(s) 389, 631, 1585, 1879, 2180
FauNDI CATATG 4 cut(s) 562, 1006, 1334, 1952
FbaI TGATCA 3 cut(s) 30, 475, 1947
FblI GTMKAC 2 cut(s) 1590, 1982
FokI GGATG 6 cut(s) 545, 790, 1562, 1772, 1806, 2324
FriOI GRGCYC 2 cut(s) 684, 1024
FspBI CTAG 4 cut(s) 1025, 1062, 1116, 2543
GlaI GCGC 3 cut(s) 470, 744, 749
GsaI CCCAGC 1 cut(s) 1572
GsuI CTGGAG 1 cut(s) 1362
HaeIII GGCC 5 cut(s) 66, 206, 1234, 1339, 2106
HapII CCGG 2 cut(s) 1257, 1794
HhaI GCGC 3 cut(s) 471, 745, 750
Hin1I GRCGYC 1 cut(s) 1979
Hin6I GCGC 3 cut(s) 469, 743, 748
HinP1I GCGC 3 cut(s) 469, 743, 748
HincII GTYRAC 2 cut(s) 592, 1983
HindII GTYRAC 2 cut(s) 592, 1983
HindIII AAGCTT 3 cut(s) 1506, 2142, 2351
HpaII CCGG 2 cut(s) 1257, 1794
HphI GGTGA 3 cut(s) 290, 1220, 2254
Hpy166II GTNNAC 5 cut(s) 281, 350, 592, 1591, 1983
Hpy188I TCNGA 9 cut(s) 146, 214, 329, 524, 1174, 2337, 2404, 2515, 2532
Hpy8I GTNNAC 5 cut(s) 281, 350, 592, 1591, 1983
Hpy99I CGWCG 1 cut(s) 1984
HpyAV CCTTC 6 cut(s) 58, 167, 782, 920, 1348, 1894
HpyCH4IV ACGT 2 cut(s) 1979, 2332
HpyF3I CTNAG 8 cut(s) 59, 188, 1097, 1385, 1690, 2074, 2336, 2381
HpySE526I ACGT 2 cut(s) 1979, 2332
Hsp92I GRCGYC 1 cut(s) 1979
HspAI GCGC 3 cut(s) 469, 743, 748
Kpn2I TCCGGA 1 cut(s) 1256
KpnI GGTACC 1 cut(s) 1712
Ksp22I TGATCA 3 cut(s) 30, 475, 1947
LguI GCTCTTC 1 cut(s) 672
LmnI GCTCC 1 cut(s) 687
Lsp1109I GCAGC 8 cut(s) 289, 1021, 1291, 1483, 1552, 2560, 2581, 2656
LweI GCATC 7 cut(s) 852, 908, 1000, 1540, 1606, 1828, 2012
MabI ACCWGGT 2 cut(s) 1593, 2372
MaeI CTAG 4 cut(s) 1025, 1062, 1116, 2543
MaeII ACGT 2 cut(s) 1979, 2332
MaeIII GTNAC 7 cut(s) 43, 729, 805, 1465, 1633, 2057, 2631
MfeI CAATTG 1 cut(s) 1314
MflI RGATCY 5 cut(s) 955, 1070, 1346, 1652, 2419
MhlI GDGCHC 4 cut(s) 352, 508, 684, 1024
MlsI TGGCCA 1 cut(s) 66
MluNI TGGCCA 1 cut(s) 66
MlyI GAGTC 3 cut(s) 866, 1453, 2264
MmeI TCCRAC 2 cut(s) 124, 1606
Mox20I TGGCCA 1 cut(s) 66
MroI TCCGGA 1 cut(s) 1256
MscI TGGCCA 1 cut(s) 66
MslI CAYNNNNRTG 5 cut(s) 72, 228, 329, 1955, 2169
Msp20I TGGCCA 1 cut(s) 66
MspA1I CMGCKG 5 cut(s) 192, 302, 485, 2551, 2647
MspCI CTTAAG 1 cut(s) 2108
MspI CCGG 2 cut(s) 1257, 1794
MspR9I CCNGG 4 cut(s) 602, 1341, 1595, 2374
MteI GCGCNGCGC 1 cut(s) 746
MunI CAATTG 1 cut(s) 1314
Mva1269I GAATGC 1 cut(s) 991
MvaI CCWGG 4 cut(s) 602, 1341, 1595, 2374
MvnI CGCG 3 cut(s) 469, 748, 2451
NdeI CATATG 4 cut(s) 562, 1006, 1334, 1952
NlaIV GGNNCC 6 cut(s) 957, 1348, 1710, 2071, 2158, 2196
NmuCI GTSAC 5 cut(s) 43, 729, 805, 1465, 2631
NspI RCATGY 2 cut(s) 728, 2604
NspV TTCGAA 3 cut(s) 812, 2297, 2557
OliI CACNNNNGTG 1 cut(s) 72
PaeI GCATGC 1 cut(s) 2604
PceI AGGCCT 1 cut(s) 2106
PciSI GCTCTTC 1 cut(s) 672
PctI GAATGC 1 cut(s) 991
PfeI GAWTC 7 cut(s) 151, 269, 335, 498, 1165, 1329, 2554
PflFI GACNNNGTC 1 cut(s) 2192
PfoI TCCNGGA 1 cut(s) 600
PinAI ACCGGT 1 cut(s) 1793
PleI GAGTC 3 cut(s) 865, 1453, 2263
PpsI GAGTC 3 cut(s) 865, 1453, 2263
PpuMI RGGWCCY 1 cut(s) 2194
PshAI GACNNNNGTC 1 cut(s) 1463
PsiI TTATAA 1 cut(s) 1578
Psp124BI GAGCTC 1 cut(s) 684
Psp5II RGGWCCY 1 cut(s) 2194
Psp6I CCWGG 4 cut(s) 600, 1339, 1593, 2372
PspEI GGTNACC 1 cut(s) 2057
PspFI CCCAGC 1 cut(s) 1568
PspGI CCWGG 4 cut(s) 600, 1339, 1593, 2372
PspN4I GGNNCC 6 cut(s) 957, 1348, 1710, 2071, 2158, 2196
PspPI GGNCC 2 cut(s) 205, 2194
PspPPI RGGWCCY 1 cut(s) 2194
PstI CTGCAG 1 cut(s) 2571
PstNI CAGNNNCTG 1 cut(s) 1034
PsuI RGATCY 5 cut(s) 955, 1070, 1346, 1652, 2419
PsyI GACNNNGTC 1 cut(s) 2192
PvuII CAGCTG 4 cut(s) 192, 302, 2551, 2647
RsaI GTAC 2 cut(s) 1710, 2651
RsaNI GTAC 2 cut(s) 1709, 2650
RseI CAYNNNNRTG 5 cut(s) 72, 228, 329, 1955, 2169
SacI GAGCTC 1 cut(s) 684
SalI GTCGAC 1 cut(s) 1981
SapI GCTCTTC 1 cut(s) 672
Sau96I GGNCC 2 cut(s) 205, 2194
SchI GAGTC 3 cut(s) 866, 1453, 2264
ScrFI CCNGG 4 cut(s) 602, 1341, 1595, 2374
SduI GDGCHC 4 cut(s) 352, 508, 684, 1024
SexAI ACCWGGT 2 cut(s) 1593, 2372
SfaNI GCATC 7 cut(s) 852, 908, 1000, 1540, 1606, 1828, 2012
SfcI CTRYAG 4 cut(s) 238, 1479, 2052, 2567
SfuI TTCGAA 3 cut(s) 812, 2297, 2557
SinI GGWCC 1 cut(s) 2194
SmiMI CAYNNNNRTG 5 cut(s) 72, 228, 329, 1955, 2169
SmlI CTYRAG 3 cut(s) 1604, 2038, 2108
SmoI CTYRAG 3 cut(s) 1604, 2038, 2108
SpeI ACTAGT 1 cut(s) 2542
SphI GCATGC 1 cut(s) 2604
SseBI AGGCCT 1 cut(s) 2106
SsiI CCGC 4 cut(s) 395, 483, 746, 2451
SspI AATATT 1 cut(s) 643
SspMI CTAG 4 cut(s) 1025, 1062, 1116, 2543
SstI GAGCTC 1 cut(s) 684
StuI AGGCCT 1 cut(s) 2106
StyD4I CCNGG 4 cut(s) 600, 1339, 1593, 2372
StyI CCWWGG 1 cut(s) 1840
TaiI ACGT 2 cut(s) 1982, 2335
TatI WGTACW 1 cut(s) 2649
TauI GCSGC 3 cut(s) 398, 485, 748
TfiI GAWTC 7 cut(s) 151, 269, 335, 498, 1165, 1329, 2554
TscAI CASTG 9 cut(s) 33, 67, 300, 478, 508, 1141, 1407, 1474, 1996
TseFI GTSAC 5 cut(s) 43, 729, 805, 1465, 2631
TseI GCWGC 8 cut(s) 302, 1034, 1279, 1496, 1565, 2548, 2569, 2644
Tsp45I GTSAC 5 cut(s) 43, 729, 805, 1465, 2631
TspGWI ACGGA 4 cut(s) 1418, 1700, 1925, 2654
TspRI CASTG 9 cut(s) 33, 67, 300, 478, 508, 1141, 1407, 1474, 1996
Tth111I GACNNNGTC 1 cut(s) 2192
Vha464I CTTAAG 1 cut(s) 2108
VneI GTGCAC 1 cut(s) 348
VpaK11BI GGWCC 1 cut(s) 2194
XapI RAATTY 2 cut(s) 841, 2095
XceI RCATGY 2 cut(s) 728, 2604
XcmI CCANNNNNNNNNTGG 1 cut(s) 313
XmiI GTMKAC 2 cut(s) 1590, 1982
XspI CTAG 4 cut(s) 1025, 1062, 1116, 2543
ZraI GACGTC 1 cut(s) 1980
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.