MD02G1233100.v1.1

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr02
OG0000294
Physical Location & Seq
Reverse (-)
27935732 .. 27939097
3366 bp
Loading structure...
UTR
Exon/CDS
Intron
MD02G1233100.v1.1.491

Sequence Viewer

Length: 2550 bp
ATGGAGTTTGATCAAAGGAAGAAGCAAGCCATCCTCTCGATATTTCTCACCTTCTTTAGCCTTGTCAGTTCTTTACATGGTAAAATAGAAGAAACTCATGTCGGGTTGATTCTTGACATGGGATCGACGGAGGGAAAGATTGTTCGAAGCTGCATTTCGGAAGCTTTATCTGATTTCTACCATCTGCATAATGACTACAGCACAAGAGTTGTTCTCCACAGTAAGGATTCCAAAGGAGAGCCTCTACATGCTCTATCATCTGTTGTCACAAACACAGCTCTGCATCTTTTGGAAAACACTAAAGTTGAAGCAATAATTGGTGCACAAACAAGAGTGGAAGCAAATCTTTTGGCAAATTTGGGAGAAGCAGCTAAAGTGCCCATAATGTCCTTTTCGGAACCTAGTCCTCCTCTTCCTATAAATGACGATAGATATGCCTTCTCTGTTGGGGTCGCACAAGATGAAACTTCTCAAGTTATGGGGATCAATGCCATCATCGAAGTTTATAAATGGAAGGATGTCATACTTCTGTATGACAATACAGAGTACGGGAATGACATGATTCCAACTTTGACAAGTTCCCTTCAACAGAAAAATGTGTATATAGCACATAAAAGTTGTGTTGCTATATCCTCAACAAATGAACAAATCTTTGAAGAACTCCAAACGCTAATGAAATTGAAGACAAAGGTATTTGTTGTGCACATTTCACACTTGCTTGTGCCTCGCCTTTTCTCTAATGTAAATAAGTTAGAAATGATGAGTGAAGGGTATACTTGGATTCTGACTTCAGCTAGCATGAATTTCTTGCATTTCATGGATTCTATTGTGATTGAATCAATGCAAGGAGTGATAGGTTTGAAGTCTTATATTCCAGCATCAACTAGGCTGCAAAATCTTACTTCAAAAGTGAGGAACAAACTTTACATGGAGGATCCAAATATGAAAGTACTTGAGTTAAGTGCAGACAAAATCTGGGTGTACGATGCAACTTGGGCTCTAGCTGAAGCAATTGAAAGAGCAAGTACTAAAAATTCTAGTAGATCCAAACATGGAGTTGTGCTACTAAGAGAAATATTGCAAAGGAGGTTTAAGGGTTTAAGTGGTGAAATTCAATTTCCAAAGGGGAAACTGATTTCGGGTACATTAGAGATAGTAAATGTGATAGGAAAAGAGGCAAGAAGGGTCGGATTTTGGTTTTTCGAAGAAAAGACCGAGAAAGCTCAGTCACACATGCTTAACAATGGGAGAAATCTTCTTTTAAGCAACCATTTGAAAACAATCAGATGGCCAGGAGGATCTAAAAGGCAATTGAGTAGTGAAATAAAATTGAGAATTGGTGTTCCAGTGAGAGTTGGGTTCAAGGAACTTGTGCGAGTGGAGCATGATCTTCAAACCAATAGAACAAATGTCACTGGGTTCAGTATCGATGTGTTCAAAACTGCAATCAGTGCGTTGCCGTATAAAGTAAGTTTTGAGTTTTTTCCATTTGAGAATGAAAATGGAGTTATGGCGGGGACTTACAGTGATCTTGTTTACCAGGTTTATCTCAAGTACGATGCTGTCGTTGGAGATATAACAATCACATCGAACAGATCTCAATATGTTGATTTTACAATGCCATATACTGACCTAGGTGTGGGAATACTAGTGCCAAAACGAAAGGACAACATGTGGATTTTCTTGAAACCTCTTTCAGCTCACCTTTGGATAACGAGTGCATGTTTCTTTATACTTACGGGTTTCATTGTGTGGTTAATTGAGCGTCCTGTCAACCAAGAATTCCAAGGTACAACTTCACAGCAAATCGGCACAATATTATGGTTCTCCTTCTCAACCCTTGCATTTTCTCATAGGGAGAAGATACTAAGCAACCTGGCAAAGTTTGTAGTGATAATATGGATGTTCGTAGTGCTTATATTGACATCCAGTTACACTGCTACTTTAGCATCAATGATGACAGTTAAACAGATACGAATGGACTCAATTGGAAACTATATAGGTTACCAATCAGGTTCATTGGGAGTTACAGTGAACTTGAATTTCAAAGAGGTTAAGCCATATCATTCAGTTGAAGAATATGCAGATGCTTTATCAAAAGGAAGCAAGCATGGTGGTGTTTCGGCCATAATTGATGAGCTTCCATATATCAATATCTTCCTTGCAAAGTATTCTTCTGACTACTCCCTCATGAAAACCAAGTCTACCACCAATGGTTTTGCTTTTGTTTTCCCTAAAGGTTCGAAATTGGTCCATGACTTATCGAAACAAATACGACATATGAGAGAAGAAGGAAAGCTTTTAGACCTGGAAGAGACCTGGTTTCCAAGAAAAAAAAGTTTTATGCCGGAGGATTATACTATAAGTAGTCCCTCCAATACTCTTGGCCTCTACAACTTTCTTGGTTTATTCCTCGTCAGCGGGGTTTCGTCTGCTGTTGCACTCTTCTTATTCGTAATCTTTTCCCATTCATTTAGAAATCTGATTAAAGGACAACTGCAGTCGGTTGGAGGACGACTGCAGCGTTTAAGGATGTTCCTTCCACTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan Lig_chan-Glu_bd SBP_bac_3

Protein Analysis

850

Amino Acids

95.86

Weight (kDa)

9.05

Isoelectric Point (pI)

38.3

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 52 - 390 6.5e-59 Receptor family ligand binding region
Peripla_BP_6 PF13458 89 - 345 8e-09 Periplasmic binding protein
Lig_chan-Glu_bd PF10613 462 - 554 7.5e-10 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 467 - 776 1.3e-13 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 567 - 809 3.6e-29 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 507
AccI GTMKAC 2 cut(s) 773, 2204
AciI CCGC 2 cut(s) 1514, 2421
AclWI GGATC 6 cut(s) 130, 491, 929, 942, 1038, 1306
AcoI YGGCCR 2 cut(s) 1289, 2124
AcsI RAATTY 6 cut(s) 355, 802, 1033, 1110, 1781, 2041
AcuI CTGAAG 2 cut(s) 774, 1026
AfaI GTAC 7 cut(s) 548, 951, 983, 1027, 1144, 1556, 1792
AfiI CCNNNNNNNGG 2 cut(s) 223, 1639
AflIII ACRYGT 1 cut(s) 1671
AhlI ACTAGT 1 cut(s) 1648
AjnI CCWGG 5 cut(s) 1291, 1539, 1875, 2307, 2318
AjuI GAANNNNNNNTTGG 2 cut(s) 300, 332
AloI GAACNNNNNNTCC 2 cut(s) 126, 158
Alw21I GWGCWC 2 cut(s) 325, 705
Alw26I GTCTC 1 cut(s) 2309
Alw44I GTGCAC 2 cut(s) 321, 701
AlwI GGATC 6 cut(s) 130, 491, 929, 942, 1038, 1306
AoxI GGCC 3 cut(s) 1289, 2124, 2386
ApaLI GTGCAC 2 cut(s) 321, 701
ApeKI GCWGC 4 cut(s) 150, 368, 889, 2521
ApoI RAATTY 6 cut(s) 355, 802, 1033, 1110, 1781, 2041
AspA2I CCTAGG 1 cut(s) 1633
AspS9I GGNCC 1 cut(s) 2251
AsuHPI GGTGA 3 cut(s) 40, 1118, 1694
AsuII TTCGAA 3 cut(s) 145, 1203, 2243
AsuNHI GCTAGC 1 cut(s) 794
AvaII GGWCC 1 cut(s) 2251
AvrII CCTAGG 1 cut(s) 1633
BaeGI GKGCMC 3 cut(s) 325, 381, 705
BalI TGGCCA 1 cut(s) 1291
BamHI GGATCC 1 cut(s) 934
BanII GRGCYC 1 cut(s) 1000
BbsI GAAGAC 1 cut(s) 689
Bbv12I GWGCWC 2 cut(s) 325, 705
BbvI GCAGC 4 cut(s) 137, 380, 876, 2533
BccI CCATC 4 cut(s) 38, 189, 500, 1281
BceAI ACGGC 1 cut(s) 1444
BcgI CGANNNNNNTGC 2 cut(s) 416, 450
BciT130I CCWGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
BclI TGATCA 1 cut(s) 10
BcoDI GTCTC 1 cut(s) 2309
BcuI ACTAGT 1 cut(s) 1648
BfaI CTAG 7 cut(s) 402, 795, 885, 1001, 1038, 1634, 1649
BfmI CTRYAG 3 cut(s) 196, 2498, 2519
BglII AGATCT 1 cut(s) 1595
BisI GCNGC 4 cut(s) 151, 369, 890, 2522
BlnI CCTAGG 1 cut(s) 1633
BlsI GCNGC 4 cut(s) 152, 370, 891, 2523
BmcAI AGTACT 2 cut(s) 951, 1027
Bme1390I CCNGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
Bme18I GGWCC 1 cut(s) 2251
BmgT120I GGNCC 1 cut(s) 2251
BmiI GGNNCC 2 cut(s) 399, 936
BmrFI CCNGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
BmrI ACTGGG 1 cut(s) 1425
BmsI GCATC 6 cut(s) 292, 887, 976, 1549, 1958, 2077
BmtI GCTAGC 1 cut(s) 798
BmuI ACTGGG 1 cut(s) 1425
BpiI GAAGAC 1 cut(s) 689
BplI GAGNNNNNCTC 2 cut(s) 198, 230
Bpu14I TTCGAA 3 cut(s) 145, 1203, 2243
BpuEI CTTGAG 3 cut(s) 456, 974, 1535
Bsa29I ATCGAT 1 cut(s) 1428
BsaI GGTCTC 1 cut(s) 2309
BsaJI CCNNGG 2 cut(s) 1633, 1786
BsaXI ACNNNNNCTCC 2 cut(s) 2016, 2046
Bsc4I CCNNNNNNNGG 2 cut(s) 223, 1639
Bse1I ACTGG 3 cut(s) 1346, 1420, 1929
BseBI CCWGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
BseCI ATCGAT 1 cut(s) 1428
BseDI CCNNGG 2 cut(s) 1633, 1786
BseGI GGATG 5 cut(s) 30, 523, 1908, 1925, 2538
BseLI CCNNNNNNNGG 2 cut(s) 223, 1639
BseMII CTCAG 1 cut(s) 1238
BseNI ACTGG 3 cut(s) 1346, 1420, 1929
BseRI GAGGAG 1 cut(s) 399
BseSI GKGCMC 3 cut(s) 325, 381, 705
BseXI GCAGC 4 cut(s) 137, 380, 876, 2533
BsgI GTGCAG 1 cut(s) 984
BshFI GGCC 3 cut(s) 1291, 2126, 2388
BshVI ATCGAT 1 cut(s) 1428
BsiHKAI GWGCWC 2 cut(s) 325, 705
BsiSI CCGG 1 cut(s) 2348
BslFI GGGAC 2 cut(s) 1531, 2355
BslI CCNNNNNNNGG 2 cut(s) 223, 1639
BsmAI GTCTC 1 cut(s) 2309
BsmFI GGGAC 2 cut(s) 1531, 2355
BsnI GGCC 3 cut(s) 1291, 2126, 2388
Bso31I GGTCTC 1 cut(s) 2309
Bsp119I TTCGAA 3 cut(s) 145, 1203, 2243
Bsp1286I GDGCHC 4 cut(s) 325, 381, 705, 1000
Bsp143I GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
BspACI CCGC 2 cut(s) 1514, 2421
BspANI GGCC 3 cut(s) 1291, 2126, 2388
BspCNI CTCAG 1 cut(s) 1237
BspDI ATCGAT 1 cut(s) 1428
BspHI TCATGA 1 cut(s) 2190
BspLI GGNNCC 2 cut(s) 399, 936
BspMAI CTGCAG 2 cut(s) 2502, 2523
BspOI GCTAGC 1 cut(s) 798
BspPI GGATC 6 cut(s) 130, 491, 929, 942, 1038, 1306
BspT104I TTCGAA 3 cut(s) 145, 1203, 2243
BspTNI GGTCTC 1 cut(s) 2309
BsrI ACTGG 3 cut(s) 1346, 1420, 1929
BssECI CCNNGG 2 cut(s) 1633, 1786
BssMI GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
BssNAI GTATAC 1 cut(s) 774
BssT1I CCWWGG 2 cut(s) 1633, 1786
Bst1107I GTATAC 1 cut(s) 774
Bst2UI CCWGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
Bst4CI ACNGT 5 cut(s) 221, 1526, 1963, 2032, 2547
Bst6I CTCTTC 3 cut(s) 417, 2307, 2450
BstAPI GCANNNNNTGC 1 cut(s) 1451
BstBI TTCGAA 3 cut(s) 145, 1203, 2243
BstC8I GCNNGC 3 cut(s) 27, 796, 2108
BstDEI CTNAG 3 cut(s) 1067, 1224, 1868
BstEII GGTNACC 1 cut(s) 2003
BstF5I GGATG 5 cut(s) 30, 523, 1908, 1925, 2538
BstKTI GATC 9 cut(s) 13, 125, 486, 937, 1046, 1301, 1390, 1531, 1598
BstMAI GTCTC 1 cut(s) 2309
BstMBI GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
BstMWI GCNNNNNNNGC 4 cut(s) 995, 1381, 1451, 1946
BstNI CCWGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
BstNSI RCATGY 4 cut(s) 251, 1237, 1675, 1725
BstPI GGTNACC 1 cut(s) 2003
BstSCI CCNGG 5 cut(s) 1291, 1539, 1875, 2307, 2318
BstSFI CTRYAG 3 cut(s) 196, 2498, 2519
BstSLI GKGCMC 3 cut(s) 325, 381, 705
BstV1I GCAGC 4 cut(s) 137, 380, 876, 2533
BstV2I GAAGAC 1 cut(s) 689
BstX2I RGATCY 4 cut(s) 934, 1043, 1298, 1595
BstYI RGATCY 4 cut(s) 934, 1043, 1298, 1595
BstZ17I GTATAC 1 cut(s) 774
Bsu15I ATCGAT 1 cut(s) 1428
BsuRI GGCC 3 cut(s) 1291, 2126, 2388
BsuTUI ATCGAT 1 cut(s) 1428
BtsCI GGATG 5 cut(s) 30, 523, 1908, 1925, 2538
BtsI GCAGTG 1 cut(s) 1935
BtsIMutI CAGTG 7 cut(s) 1353, 1413, 1456, 1531, 1935, 2037, 2543
Cac8I GCNNGC 3 cut(s) 27, 796, 2108
CciI TCATGA 1 cut(s) 2190
Cfr13I GGNCC 1 cut(s) 2251
ClaI ATCGAT 1 cut(s) 1428
CseI GACGC 1 cut(s) 1754
CsiI ACCWGGT 2 cut(s) 1539, 2318
Csp6I GTAC 7 cut(s) 547, 950, 982, 1026, 1143, 1555, 1791
CspCI CAANNNNNGTGG 2 cut(s) 2095, 2130
CviQI GTAC 7 cut(s) 547, 950, 982, 1026, 1143, 1555, 1791
DdeI CTNAG 3 cut(s) 1067, 1224, 1868
DpnI GATC 9 cut(s) 12, 124, 485, 936, 1045, 1300, 1389, 1530, 1597
DpnII GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
EaeI YGGCCR 2 cut(s) 1289, 2124
Eam1104I CTCTTC 3 cut(s) 417, 2307, 2450
EarI CTCTTC 3 cut(s) 417, 2307, 2450
Eco130I CCWWGG 2 cut(s) 1633, 1786
Eco24I GRGCYC 1 cut(s) 1000
Eco31I GGTCTC 1 cut(s) 2309
Eco47I GGWCC 1 cut(s) 2251
Eco57I CTGAAG 2 cut(s) 774, 1026
Eco91I GGTNACC 1 cut(s) 2003
EcoO65I GGTNACC 1 cut(s) 2003
EcoRI GAATTC 1 cut(s) 1781
EcoRII CCWGG 5 cut(s) 1291, 1539, 1875, 2307, 2318
EcoT14I CCWWGG 2 cut(s) 1633, 1786
EcoT38I GRGCYC 1 cut(s) 1000
ErhI CCWWGG 2 cut(s) 1633, 1786
FalI AAGNNNNNCTT 4 cut(s) 330, 362, 2283, 2315
FaqI GGGAC 2 cut(s) 1531, 2355
FauI CCCGC 2 cut(s) 1507, 2414
FauNDI CATATG 1 cut(s) 2280
FbaI TGATCA 1 cut(s) 10
FblI GTMKAC 2 cut(s) 773, 2204
Fnu4HI GCNGC 4 cut(s) 151, 369, 890, 2522
FokI GGATG 5 cut(s) 17, 530, 1912, 1915, 2545
FriOI GRGCYC 1 cut(s) 1000
Fsp4HI GCNGC 4 cut(s) 151, 369, 890, 2522
FspBI CTAG 7 cut(s) 402, 795, 885, 1001, 1038, 1634, 1649
GluI GCNGC 4 cut(s) 151, 369, 890, 2522
HaeIII GGCC 3 cut(s) 1291, 2126, 2388
HapII CCGG 1 cut(s) 2348
HgaI GACGC 1 cut(s) 1754
HincII GTYRAC 1 cut(s) 1774
HindII GTYRAC 1 cut(s) 1774
HindIII AAGCTT 2 cut(s) 162, 2297
HinfI GANTC 7 cut(s) 109, 227, 562, 781, 821, 836, 1982
HpaII CCGG 1 cut(s) 2348
HphI GGTGA 3 cut(s) 40, 1118, 1694
Hpy166II GTNNAC 8 cut(s) 323, 703, 774, 982, 1537, 1774, 2035, 2205
Hpy188I TCNGA 8 cut(s) 160, 172, 397, 786, 1190, 1286, 2179, 2484
Hpy188III TCNNGA 4 cut(s) 37, 113, 1684, 2191
Hpy8I GTNNAC 8 cut(s) 323, 703, 774, 982, 1537, 1774, 2035, 2205
Hpy99I CGWCG 1 cut(s) 130
HpyAV CCTTC 9 cut(s) 61, 448, 508, 593, 761, 1176, 1840, 2285, 2549
HpyCH4III ACNGT 5 cut(s) 221, 1526, 1963, 2032, 2547
HpyF10VI GCNNNNNNNGC 4 cut(s) 995, 1381, 1451, 1946
HpyF3I CTNAG 3 cut(s) 1067, 1224, 1868
Ksp22I TGATCA 1 cut(s) 10
Kzo9I GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
LmnI GCTCC 1 cut(s) 1381
Lsp1109I GCAGC 4 cut(s) 137, 380, 876, 2533
LweI GCATC 6 cut(s) 292, 887, 976, 1549, 1958, 2077
MabI ACCWGGT 2 cut(s) 1539, 2318
MaeI CTAG 7 cut(s) 402, 795, 885, 1001, 1038, 1634, 1649
MaeIII GTNAC 6 cut(s) 265, 1227, 1411, 1931, 2003, 2026
MalI GATC 9 cut(s) 12, 124, 485, 936, 1045, 1300, 1389, 1530, 1597
MboI GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
MfeI CAATTG 3 cut(s) 1011, 1310, 1986
MflI RGATCY 4 cut(s) 934, 1043, 1298, 1595
MhlI GDGCHC 4 cut(s) 325, 381, 705, 1000
MlsI TGGCCA 1 cut(s) 1291
MluNI TGGCCA 1 cut(s) 1291
MlyI GAGTC 1 cut(s) 1976
MmeI TCCRAC 4 cut(s) 590, 1168, 1549, 2488
Mox20I TGGCCA 1 cut(s) 1291
MscI TGGCCA 1 cut(s) 1291
MslI CAYNNNNRTG 1 cut(s) 2115
Msp20I TGGCCA 1 cut(s) 1291
MspA1I CMGCKG 1 cut(s) 2421
MspI CCGG 1 cut(s) 2348
MspR9I CCNGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
MunI CAATTG 3 cut(s) 1011, 1310, 1986
MvaI CCWGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
MwoI GCNNNNNNNGC 4 cut(s) 995, 1381, 1451, 1946
NdeI CATATG 1 cut(s) 2280
NdeII GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
NheI GCTAGC 1 cut(s) 794
NlaIV GGNNCC 2 cut(s) 399, 936
NmuCI GTSAC 3 cut(s) 265, 1227, 1411
NspI RCATGY 4 cut(s) 251, 1237, 1675, 1725
NspV TTCGAA 3 cut(s) 145, 1203, 2243
PagI TCATGA 1 cut(s) 2190
PciI ACATGT 1 cut(s) 1671
PcsI WCGNNNNNNNCGW 2 cut(s) 1563, 2521
PfeI GAWTC 6 cut(s) 109, 227, 562, 781, 821, 836
PkrI GCNGC 4 cut(s) 152, 370, 891, 2523
PleI GAGTC 1 cut(s) 1976
PpsI GAGTC 1 cut(s) 1976
PscI ACATGT 1 cut(s) 1671
PsiI TTATAA 1 cut(s) 507
Psp6I CCWGG 5 cut(s) 1291, 1539, 1875, 2307, 2318
PspEI GGTNACC 1 cut(s) 2003
PspGI CCWGG 5 cut(s) 1291, 1539, 1875, 2307, 2318
PspN4I GGNNCC 2 cut(s) 399, 936
PspPI GGNCC 1 cut(s) 2251
PsrI GAACNNNNNNTAC 2 cut(s) 908, 940
PstI CTGCAG 2 cut(s) 2502, 2523
PsuI RGATCY 4 cut(s) 934, 1043, 1298, 1595
RsaI GTAC 7 cut(s) 548, 951, 983, 1027, 1144, 1556, 1792
RsaNI GTAC 7 cut(s) 547, 950, 982, 1026, 1143, 1555, 1791
RseI CAYNNNNRTG 1 cut(s) 2115
SatI GCNGC 4 cut(s) 151, 369, 890, 2522
Sau3AI GATC 9 cut(s) 10, 122, 483, 934, 1043, 1298, 1387, 1528, 1595
Sau96I GGNCC 1 cut(s) 2251
ScaI AGTACT 2 cut(s) 951, 1027
SchI GAGTC 1 cut(s) 1976
ScrFI CCNGG 5 cut(s) 1293, 1541, 1877, 2309, 2320
SduI GDGCHC 4 cut(s) 325, 381, 705, 1000
SexAI ACCWGGT 2 cut(s) 1539, 2318
SfaNI GCATC 6 cut(s) 292, 887, 976, 1549, 1958, 2077
SfcI CTRYAG 3 cut(s) 196, 2498, 2519
SfuI TTCGAA 3 cut(s) 145, 1203, 2243
SinI GGWCC 1 cut(s) 2251
SmiMI CAYNNNNRTG 1 cut(s) 2115
SmlI CTYRAG 3 cut(s) 471, 953, 1550
SmoI CTYRAG 3 cut(s) 471, 953, 1550
SpeI ACTAGT 1 cut(s) 1648
SsiI CCGC 2 cut(s) 1514, 2421
SspI AATATT 2 cut(s) 1077, 1818
SspMI CTAG 7 cut(s) 402, 795, 885, 1001, 1038, 1634, 1649
StyD4I CCNGG 5 cut(s) 1291, 1539, 1875, 2307, 2318
StyI CCWWGG 2 cut(s) 1633, 1786
TaaI ACNGT 5 cut(s) 221, 1526, 1963, 2032, 2547
TaqI TCGA 9 cut(s) 38, 125, 145, 498, 1203, 1428, 1589, 2243, 2264
TaqII GACCGA 1 cut(s) 1229
TatI WGTACW 2 cut(s) 949, 1025
TfiI GAWTC 6 cut(s) 109, 227, 562, 781, 821, 836
TscAI CASTG 7 cut(s) 1353, 1420, 1456, 1531, 1942, 2037, 2550
TseFI GTSAC 3 cut(s) 265, 1227, 1411
TseI GCWGC 4 cut(s) 150, 368, 889, 2521
Tsp45I GTSAC 3 cut(s) 265, 1227, 1411
TspGWI ACGGA 1 cut(s) 143
TspRI CASTG 7 cut(s) 1353, 1420, 1456, 1531, 1942, 2037, 2550
VneI GTGCAC 2 cut(s) 321, 701
VpaK11BI GGWCC 1 cut(s) 2251
XapI RAATTY 6 cut(s) 355, 802, 1033, 1110, 1781, 2041
XceI RCATGY 4 cut(s) 251, 1237, 1675, 1725
XmaJI CCTAGG 1 cut(s) 1633
XmiI GTMKAC 2 cut(s) 773, 2204
XspI CTAG 7 cut(s) 402, 795, 885, 1001, 1038, 1634, 1649
ZrmI AGTACT 2 cut(s) 951, 1027
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.