Rroxscaffold_4G00312820

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000294
Physical Location & Seq
Reverse (-)
36052200 .. 36057969
5770 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312820.1

Sequence Viewer

Length: 2673 bp
ATGGAGTTTCGAGGGTTGAGGCAAGCAATAATCATCCCGTCCTTTGTGACCTTCTGTTGCCTCATTGGTCATTTAAGTGCTGATATTGAAAAGTTAAACTATGATGTTGATTCTATTATCGTTGAAGATCAGGTTCATGTTGGTGTGATTCTCGATAAGGAATCAAGGGAAGGAAAGATTGTTCTCAGCTGCATTTCAATGGCCCTCTCTGATTTTTACCATCTGCACTATAACTACAGGACAAGAATAGTTCTCTACAGTAAGGATTCCAAAGGTGAACCTCTGCCTGCTCTGTCAGCTGCTCTTGACTTTTTGGAAAATATCAAAGTGGAAGTAATAATTGGTGCACCAAAAAGAGCAGAAGCAAATCTTCTGGCAGAATTAGGAGAAGCGGCCAAACTCCCTTTAATGTCTCTTTCTCCTGTTCGGACTGACAATAAATATCCCTTCTTTGTTGAGATCATGCGCGCTGATGAAGCGGCTGAAGTTAAGGGAATTAGTGCCATTATTGAAGTTTTCAAATGGAGGGATGTTATCCTTTTATATGATAGCAAAGAATATGAGAGAGACTTCATTCCATCATTAGGCAAGTCATTCCAAGAGTTCACACATGGGAGAGTTGCGTACAAAAGTTCCAATATTGCTTCCTCCTCATCAAATGAAGAAATCACTGCAGAGCTCCAAAAGTTGATCAACCTGAAGATTAAGGTATTTGTGGTGCATGTGTCACATTTTTTTGCGCCTCATCTTTTCTTGAATGCAAAGAAGTTAGGGATGATGAGCGAAGGGTATGCTTGGATCATGACATCAACTAGCATGAATTTCTTGCATTCTATGAATTTATCTGTTATTGAGTCAATGCAAGGAGTGGTGGGTTTCAAGTCTTCTATTCCAGCGTCAATAAACCTTCACGGTCTTACATCAAGATTGAGGAGGAAGTTTTACATTGATGATCCAAATATGGGAGCAATTCGGGAGTTGAGTGCAGATGGGATCTGGGCATATGATGCAACTTGGGCTCTAGCAGAAGCAGTTGAAAGAGCAAGGCTTAAAAATTCTACCACTAGATCCTCCAAAGATGGAGCTGTGCTTCTTAGAGAGATATTGCAGACTAGATTTAAAGGTTTAAGTGGTGAAATTCAATATGTGAATGGGAAACTGATTCCATCAGAACCATTTGAGATAGTCAATGTTATTGGAAAAGGTGAGATCAAAAGGATTGGATTTTGGCCTTGCAAAGAAGAAGTAAAATCCAGAAAAGGGTCACCCCAGCAGCAACTCATTCATAATGGGAGAAATTTAGCTTCTACAATTGATCTAGAAAGAATCATATGGCCTGGAGGTTCCAGGAGGGAATTGAGTAGTAGTGAAACACTTAAACTAAAAATTGGTGTTCCAGTGAGAATTGGGTTCAAGGAACTTGTTTGTGTCGAGCATGATCTTGAAACCAATACAACTCTTGTCACTGGCTTCTCTATAGATGTATTCAAAGCTGCAATAGGAGCTCTGCCATATGAAGTGCTGTACGAGTTTATTCCATTTGAGGATGCCAATGGAAATCCTGCAGGGACTTATAATGATCTTGTTTACCAGGTTTATCTCAAGAAATACGATGCTGTTGTTGGAGATGTTACGATCACAGAGAACAGATCTCAGTATGTTGATTTTACAATCCCATATACTGACTTGGGTGTGGGACTGTTGGTACCAAATGCCAAGGGAAACATGTGGATTTTCTTTAAACCACTTTCAACATATCTTTGGATAACAAGTGCTGCTTTCTTTATCCTAACCAGTTTTGTTGTTTGGATCATTGAGCGTCCTACTAATAAAGAATTCCAGGGCACAGTAGAGGAGCAAATCGGAACAGTTTTCTGGTTCTCCTTTTCTACTCTTGTGTTTGCTTATACGGAAAAGTTGTCGAATAATTTGGCAAAGTTTGTAGTGATAATATGGGTGTTTGTAGTGCTTATATTGTCATCAAGTTATACTGCAACTTTAGCATCAATGATGACGGTCAAACAGATTGAATTAAACTCGAGAGGGAACTATATAGGTTATCAATCGGGTTCATTAGGAGTTGTAGTGAACTTAAATTTGAAAGCAGCTAAGTCATATCATTCAGAAGAAGAATATATTGATGCTCTATCTAAAGGAAGCAAGAACGGTGGAGTTTCTGGGATTATTGACGAGGTTCCGTACATCAATATCTTCCTTGCAAAGTATTCTGAGGACTACTCGATGATTAAAACCAGGTCTATCACCAATGGTTTTGCCTTTGTTTTCCCGAAAGGTTCGAAATTGGTTCAAGATGTGTCAAGGCAAATTGAACGTCTGAGACAGCAAGGAAAGCTTCTAGAGATGGAAAAGACCTGGTTTCGTGAACAAATAACTCTCCTGTCTGATGAGGATATAACCCAGAGTGATCCCAATACGATTGACCTCTATGACTTTCGTGGTCTGTTCCTTATTAGTGGGGCTTCTTTAGCTATTTCTCTTTTCTTCTTCATAGTTTTATCCACTAGATTTAGGAATCTGTTTCAAGGACTAATGCAGCTGATTCGTATAGAACTGCAACGTTTGAGAACTTTCTTCTCCTTCATAGTATTCAATGGAATTGAGCAAAACTGCATGCAAGTTCCTACGCAAGTCATTGTACATGCTGTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

890

Amino Acids

100.42

Weight (kDa)

6.48

Isoelectric Point (pI)

34.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ANF_receptor PF01094 63 - 399 5.6e-57 Receptor family ligand binding region
Peripla_BP_6 PF13458 80 - 398 7.9e-13 Periplasmic binding protein
Lig_chan-Glu_bd PF10613 479 - 571 2.2e-09 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 488 - 794 6.4e-13 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 585 - 827 2.1e-26 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1575
Acc65I GGTACC 1 cut(s) 1705
AccB1I GGYRCC 1 cut(s) 1705
AccII CGCG 1 cut(s) 468
AciI CCGC 2 cut(s) 392, 479
AclI AACGTT 1 cut(s) 2578
AclWI GGATC 6 cut(s) 806, 947, 1001, 1062, 1817, 2420
AcoI YGGCCR 1 cut(s) 393
AcsI RAATTY 7 cut(s) 820, 838, 1054, 1137, 1297, 1835, 2095
AcuI CTGAAG 2 cut(s) 504, 719
AfaI GTAC 5 cut(s) 626, 1526, 1707, 2201, 2658
AfiI CCNNNNNNNGG 3 cut(s) 584, 962, 1260
AflIII ACRYGT 1 cut(s) 1725
AjnI CCWGG 6 cut(s) 1336, 1346, 1590, 1839, 2252, 2372
AjuI GAANNNNNNNTTGG 2 cut(s) 324, 356
AloI GAACNNNNNNTCC 2 cut(s) 165, 197
Alw21I GWGCWC 3 cut(s) 349, 681, 1507
Alw26I GTCTC 3 cut(s) 417, 561, 2332
Alw44I GTGCAC 1 cut(s) 345
AlwI GGATC 6 cut(s) 806, 947, 1001, 1062, 1817, 2420
Ama87I CYCGRG 1 cut(s) 2038
AoxI GGCC 4 cut(s) 201, 393, 1229, 1334
ApaLI GTGCAC 1 cut(s) 345
ApeKI GCWGC 7 cut(s) 189, 299, 1273, 1493, 1775, 2105, 2554
ApoI RAATTY 7 cut(s) 820, 838, 1054, 1137, 1297, 1835, 2095
ArsI GACNNNNNNTTYG 2 cut(s) 2317, 2349
Asp718I GGTACC 1 cut(s) 1705
AspLEI GCGC 3 cut(s) 468, 470, 742
AspS9I GGNCC 1 cut(s) 202
AsuHPI GGTGA 5 cut(s) 287, 1145, 1217, 1257, 2254
AsuII TTCGAA 1 cut(s) 2297
AvaI CYCGRG 1 cut(s) 2038
BaeGI GKGCMC 2 cut(s) 349, 1847
BanI GGYRCC 1 cut(s) 1705
BanII GRGCYC 3 cut(s) 681, 1021, 1507
BbsI GAAGAC 1 cut(s) 876
Bbv12I GWGCWC 3 cut(s) 349, 681, 1507
BbvI GCAGC 7 cut(s) 176, 286, 1285, 1480, 1762, 2117, 2566
BccI CCATC 6 cut(s) 228, 586, 983, 1073, 1174, 2356
BcgI CGANNNNNNTGC 4 cut(s) 773, 807, 2543, 2577
BciT130I CCWGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
BclI TGATCA 1 cut(s) 690
BcoDI GTCTC 3 cut(s) 417, 561, 2332
BfaI CTAG 7 cut(s) 813, 1022, 1065, 1113, 1319, 2357, 2523
BfmI CTRYAG 5 cut(s) 235, 256, 672, 1476, 1563
BglII AGATCT 1 cut(s) 1649
BisI GCNGC 9 cut(s) 190, 300, 393, 480, 1274, 1494, 1776, 2106, 2555
BlsI GCNGC 9 cut(s) 191, 301, 394, 481, 1275, 1495, 1777, 2107, 2556
Bme1390I CCNGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
BmeT110I CYCGRG 1 cut(s) 2038
BmgT120I GGNCC 1 cut(s) 202
BmiI GGNNCC 3 cut(s) 1345, 1707, 2196
BmrFI CCNGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
BmsI GCATC 5 cut(s) 997, 1537, 1603, 2012, 2131
BpiI GAAGAC 1 cut(s) 876
BplI GAGNNNNNCTC 2 cut(s) 2222, 2254
BpmI CTGGAG 1 cut(s) 1359
Bpu14I TTCGAA 1 cut(s) 2297
BpuEI CTTGAG 1 cut(s) 1586
BsaJI CCNNGG 2 cut(s) 1716, 1840
BsaXI ACNNNNNCTCC 2 cut(s) 2070, 2100
Bsc4I CCNNNNNNNGG 3 cut(s) 584, 962, 1260
Bse1I ACTGG 3 cut(s) 1397, 1471, 1794
BseBI CCWGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
BseDI CCNNGG 2 cut(s) 1716, 1840
BseGI GGATG 4 cut(s) 33, 535, 780, 1552
BseLI CCNNNNNNNGG 3 cut(s) 584, 962, 1260
BseMII CTCAG 4 cut(s) 199, 1667, 2220, 2327
BseNI ACTGG 3 cut(s) 1397, 1471, 1794
BsePI GCGCGC 1 cut(s) 466
BseRI GAGGAG 3 cut(s) 640, 946, 1868
BseSI GKGCMC 2 cut(s) 349, 1847
BseXI GCAGC 7 cut(s) 176, 286, 1285, 1480, 1762, 2117, 2566
BseYI CCCAGC 1 cut(s) 1269
BsgI GTGCAG 2 cut(s) 209, 1005
Bsh1236I CGCG 1 cut(s) 468
BshFI GGCC 4 cut(s) 203, 395, 1231, 1336
BshNI GGYRCC 1 cut(s) 1705
BsiHKAI GWGCWC 3 cut(s) 349, 681, 1507
BsiHKCI CYCGRG 1 cut(s) 2038
BslFI GGGAC 2 cut(s) 1582, 1710
BslI CCNNNNNNNGG 3 cut(s) 584, 962, 1260
BsmAI GTCTC 3 cut(s) 417, 561, 2332
BsmFI GGGAC 2 cut(s) 1582, 1710
BsmI GAATGC 2 cut(s) 763, 829
BsnI GGCC 4 cut(s) 203, 395, 1231, 1336
BsoBI CYCGRG 1 cut(s) 2038
Bsp119I TTCGAA 1 cut(s) 2297
Bsp1286I GDGCHC 5 cut(s) 349, 681, 1021, 1507, 1847
Bsp1407I TGTACA 1 cut(s) 2656
BspACI CCGC 2 cut(s) 392, 479
BspANI GGCC 4 cut(s) 203, 395, 1231, 1336
BspCNI CTCAG 4 cut(s) 198, 1666, 2221, 2328
BspFNI CGCG 1 cut(s) 468
BspHI TCATGA 1 cut(s) 801
BspLI GGNNCC 3 cut(s) 1345, 1707, 2196
BspMAI CTGCAG 2 cut(s) 676, 1567
BspPI GGATC 6 cut(s) 806, 947, 1001, 1062, 1817, 2420
BspT104I TTCGAA 1 cut(s) 2297
BspT107I GGYRCC 1 cut(s) 1705
BsrGI TGTACA 1 cut(s) 2656
BsrI ACTGG 3 cut(s) 1397, 1471, 1794
BssECI CCNNGG 2 cut(s) 1716, 1840
BssHII GCGCGC 1 cut(s) 466
BssT1I CCWWGG 1 cut(s) 1716
Bst2UI CCWGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
Bst4CI ACNGT 7 cut(s) 260, 914, 1701, 1849, 1870, 2017, 2168
BstAPI GCANNNNNTGC 1 cut(s) 1007
BstAUI TGTACA 1 cut(s) 2656
BstBI TTCGAA 1 cut(s) 2297
BstC8I GCNNGC 4 cut(s) 24, 288, 468, 2633
BstDEI CTNAG 6 cut(s) 185, 1094, 1653, 2109, 2229, 2336
BstEII GGTNACC 1 cut(s) 1263
BstF5I GGATG 4 cut(s) 33, 535, 780, 1552
BstFNI CGCG 1 cut(s) 468
BstHHI GCGC 3 cut(s) 468, 470, 742
BstMAI GTCTC 3 cut(s) 417, 561, 2332
BstMWI GCNNNNNNNGC 8 cut(s) 296, 476, 1007, 1016, 1502, 2000, 2350, 2486
BstNI CCWGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
BstNSI RCATGY 4 cut(s) 725, 1729, 2635, 2663
BstPI GGTNACC 1 cut(s) 1263
BstSCI CCNGG 6 cut(s) 1336, 1346, 1590, 1839, 2252, 2372
BstSFI CTRYAG 5 cut(s) 235, 256, 672, 1476, 1563
BstSLI GKGCMC 2 cut(s) 349, 1847
BstUI CGCG 1 cut(s) 468
BstV1I GCAGC 7 cut(s) 176, 286, 1285, 1480, 1762, 2117, 2566
BstV2I GAAGAC 1 cut(s) 876
BstX2I RGATCY 3 cut(s) 993, 1067, 1649
BstYI RGATCY 3 cut(s) 993, 1067, 1649
BsuRI GGCC 4 cut(s) 203, 395, 1231, 1336
BtsCI GGATG 4 cut(s) 33, 535, 780, 1552
BtsI GCAGTG 1 cut(s) 669
BtsIMutI CAGTG 3 cut(s) 669, 1404, 1464
Cac8I GCNNGC 4 cut(s) 24, 288, 468, 2633
CciI TCATGA 1 cut(s) 801
CfoI GCGC 3 cut(s) 468, 470, 742
Cfr13I GGNCC 1 cut(s) 202
CseI GACGC 2 cut(s) 885, 1808
CsiI ACCWGGT 3 cut(s) 1590, 2252, 2372
Csp6I GTAC 5 cut(s) 625, 1525, 1706, 2200, 2657
CspCI CAANNNNNGTGG 2 cut(s) 2149, 2184
CviQI GTAC 5 cut(s) 625, 1525, 1706, 2200, 2657
DdeI CTNAG 6 cut(s) 185, 1094, 1653, 2109, 2229, 2336
DraI TTTAAA 2 cut(s) 1120, 1741
EaeI YGGCCR 1 cut(s) 393
Ecl136II GAGCTC 2 cut(s) 679, 1505
Eco130I CCWWGG 1 cut(s) 1716
Eco24I GRGCYC 3 cut(s) 681, 1021, 1507
Eco53kI GAGCTC 2 cut(s) 679, 1505
Eco57I CTGAAG 2 cut(s) 504, 719
Eco88I CYCGRG 1 cut(s) 2038
Eco91I GGTNACC 1 cut(s) 1263
EcoICRI GAGCTC 2 cut(s) 679, 1505
EcoO65I GGTNACC 1 cut(s) 1263
EcoRI GAATTC 1 cut(s) 1835
EcoRII CCWGG 6 cut(s) 1336, 1346, 1590, 1839, 2252, 2372
EcoT14I CCWWGG 1 cut(s) 1716
EcoT38I GRGCYC 3 cut(s) 681, 1021, 1507
ErhI CCWWGG 1 cut(s) 1716
FalI AAGNNNNNCTT 6 cut(s) 354, 386, 1762, 1794, 2337, 2369
FaqI GGGAC 2 cut(s) 1582, 1710
FauNDI CATATG 3 cut(s) 1003, 1331, 1513
FbaI TGATCA 1 cut(s) 690
Fnu4HI GCNGC 9 cut(s) 190, 300, 393, 480, 1274, 1494, 1776, 2106, 2555
FokI GGATG 4 cut(s) 20, 542, 787, 1559
FriOI GRGCYC 3 cut(s) 681, 1021, 1507
Fsp4HI GCNGC 9 cut(s) 190, 300, 393, 480, 1274, 1494, 1776, 2106, 2555
FspBI CTAG 7 cut(s) 813, 1022, 1065, 1113, 1319, 2357, 2523
GlaI GCGC 3 cut(s) 467, 469, 741
GluI GCNGC 9 cut(s) 190, 300, 393, 480, 1274, 1494, 1776, 2106, 2555
GsaI CCCAGC 1 cut(s) 1273
GsuI CTGGAG 1 cut(s) 1359
HaeIII GGCC 4 cut(s) 203, 395, 1231, 1336
HgaI GACGC 2 cut(s) 885, 1808
HhaI GCGC 3 cut(s) 468, 470, 742
Hin6I GCGC 3 cut(s) 466, 468, 740
HinP1I GCGC 3 cut(s) 466, 468, 740
HindIII AAGCTT 1 cut(s) 2351
HinfI GANTC 9 cut(s) 110, 148, 161, 266, 854, 1162, 1326, 2533, 2560
HphI GGTGA 5 cut(s) 287, 1145, 1217, 1257, 2254
Hpy166II GTNNAC 6 cut(s) 278, 347, 606, 1588, 2089, 2384
Hpy188I TCNGA 8 cut(s) 211, 429, 1171, 1865, 2125, 2230, 2337, 2404
Hpy8I GTNNAC 6 cut(s) 278, 347, 606, 1588, 2089, 2384
HpyAV CCTTC 6 cut(s) 61, 164, 457, 779, 917, 2608
HpyCH4III ACNGT 7 cut(s) 260, 914, 1701, 1849, 1870, 2017, 2168
HpyCH4IV ACGT 2 cut(s) 2332, 2578
HpyF10VI GCNNNNNNNGC 8 cut(s) 296, 476, 1007, 1016, 1502, 2000, 2350, 2486
HpyF3I CTNAG 6 cut(s) 185, 1094, 1653, 2109, 2229, 2336
HpySE526I ACGT 2 cut(s) 2332, 2578
HspAI GCGC 3 cut(s) 466, 468, 740
KpnI GGTACC 1 cut(s) 1709
Ksp22I TGATCA 1 cut(s) 690
LmnI GCTCC 5 cut(s) 684, 965, 1082, 1502, 1855
Lsp1109I GCAGC 7 cut(s) 176, 286, 1285, 1480, 1762, 2117, 2566
LweI GCATC 5 cut(s) 997, 1537, 1603, 2012, 2131
MabI ACCWGGT 3 cut(s) 1590, 2252, 2372
MaeI CTAG 7 cut(s) 813, 1022, 1065, 1113, 1319, 2357, 2523
MaeII ACGT 2 cut(s) 2332, 2578
MaeIII GTNAC 5 cut(s) 46, 726, 1263, 1462, 1630
MfeI CAATTG 1 cut(s) 1311
MflI RGATCY 3 cut(s) 993, 1067, 1649
MhlI GDGCHC 5 cut(s) 349, 681, 1021, 1507, 1847
MlyI GAGTC 1 cut(s) 863
MmeI TCCRAC 1 cut(s) 1603
MslI CAYNNNNRTG 3 cut(s) 75, 141, 197
MspA1I CMGCKG 3 cut(s) 189, 299, 2557
MspR9I CCNGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
MunI CAATTG 1 cut(s) 1311
Mva1269I GAATGC 2 cut(s) 763, 829
MvaI CCWGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
MvnI CGCG 1 cut(s) 468
MwoI GCNNNNNNNGC 8 cut(s) 296, 476, 1007, 1016, 1502, 2000, 2350, 2486
NdeI CATATG 3 cut(s) 1003, 1331, 1513
NlaIV GGNNCC 3 cut(s) 1345, 1707, 2196
NmuCI GTSAC 4 cut(s) 46, 726, 1263, 1462
NspI RCATGY 4 cut(s) 725, 1729, 2635, 2663
NspV TTCGAA 1 cut(s) 2297
PaeI GCATGC 1 cut(s) 2635
PaeR7I CTCGAG 1 cut(s) 2038
PagI TCATGA 1 cut(s) 801
PauI GCGCGC 1 cut(s) 466
PciI ACATGT 1 cut(s) 1725
PctI GAATGC 2 cut(s) 763, 829
PfeI GAWTC 8 cut(s) 110, 148, 161, 266, 1162, 1326, 2533, 2560
PfoI TCCNGGA 1 cut(s) 1346
PkrI GCNGC 9 cut(s) 191, 301, 394, 481, 1275, 1495, 1777, 2107, 2556
PleI GAGTC 1 cut(s) 862
PpsI GAGTC 1 cut(s) 862
PscI ACATGT 1 cut(s) 1725
PsiI TTATAA 1 cut(s) 1575
Psp124BI GAGCTC 2 cut(s) 681, 1507
Psp1406I AACGTT 1 cut(s) 2578
Psp6I CCWGG 6 cut(s) 1336, 1346, 1590, 1839, 2252, 2372
PspEI GGTNACC 1 cut(s) 1263
PspFI CCCAGC 1 cut(s) 1269
PspGI CCWGG 6 cut(s) 1336, 1346, 1590, 1839, 2252, 2372
PspN4I GGNNCC 3 cut(s) 1345, 1707, 2196
PspPI GGNCC 1 cut(s) 202
PstI CTGCAG 2 cut(s) 676, 1567
PsuI RGATCY 3 cut(s) 993, 1067, 1649
PteI GCGCGC 1 cut(s) 466
PvuII CAGCTG 3 cut(s) 189, 299, 2557
RsaI GTAC 5 cut(s) 626, 1526, 1707, 2201, 2658
RsaNI GTAC 5 cut(s) 625, 1525, 1706, 2200, 2657
RseI CAYNNNNRTG 3 cut(s) 75, 141, 197
SacI GAGCTC 2 cut(s) 681, 1507
SatI GCNGC 9 cut(s) 190, 300, 393, 480, 1274, 1494, 1776, 2106, 2555
Sau96I GGNCC 1 cut(s) 202
SbfI CCTGCAGG 1 cut(s) 1567
SchI GAGTC 1 cut(s) 863
ScrFI CCNGG 6 cut(s) 1338, 1348, 1592, 1841, 2254, 2374
SdaI CCTGCAGG 1 cut(s) 1567
SduI GDGCHC 5 cut(s) 349, 681, 1021, 1507, 1847
SexAI ACCWGGT 3 cut(s) 1590, 2252, 2372
SfaNI GCATC 5 cut(s) 997, 1537, 1603, 2012, 2131
SfcI CTRYAG 5 cut(s) 235, 256, 672, 1476, 1563
Sfr274I CTCGAG 1 cut(s) 2038
SfuI TTCGAA 1 cut(s) 2297
SlaI CTCGAG 1 cut(s) 2038
SmiMI CAYNNNNRTG 3 cut(s) 75, 141, 197
SmlI CTYRAG 2 cut(s) 1601, 2038
SmoI CTYRAG 2 cut(s) 1601, 2038
SphI GCATGC 1 cut(s) 2635
Sse8387I CCTGCAGG 1 cut(s) 1567
SsiI CCGC 2 cut(s) 392, 479
SspI AATATT 1 cut(s) 640
SspMI CTAG 7 cut(s) 813, 1022, 1065, 1113, 1319, 2357, 2523
SstI GAGCTC 2 cut(s) 681, 1507
StyD4I CCNGG 6 cut(s) 1336, 1346, 1590, 1839, 2252, 2372
StyI CCWWGG 1 cut(s) 1716
TaaI ACNGT 7 cut(s) 260, 914, 1701, 1849, 1870, 2017, 2168
TaiI ACGT 2 cut(s) 2335, 2581
TaqI TCGA 7 cut(s) 10, 153, 1431, 1922, 2039, 2240, 2297
TatI WGTACW 1 cut(s) 2656
TauI GCSGC 2 cut(s) 395, 482
TfiI GAWTC 8 cut(s) 110, 148, 161, 266, 1162, 1326, 2533, 2560
TscAI CASTG 3 cut(s) 676, 1404, 1471
TseFI GTSAC 4 cut(s) 46, 726, 1263, 1462
TseI GCWGC 7 cut(s) 189, 299, 1273, 1493, 1775, 2105, 2554
Tsp45I GTSAC 4 cut(s) 46, 726, 1263, 1462
TspGWI ACGGA 2 cut(s) 1925, 2187
TspRI CASTG 3 cut(s) 676, 1404, 1471
VneI GTGCAC 1 cut(s) 345
XapI RAATTY 7 cut(s) 820, 838, 1054, 1137, 1297, 1835, 2095
XbaI TCTAGA 2 cut(s) 1318, 2356
XceI RCATGY 4 cut(s) 725, 1729, 2635, 2663
XhoI CTCGAG 1 cut(s) 2038
XspI CTAG 7 cut(s) 813, 1022, 1065, 1113, 1319, 2357, 2523
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.