Rh1BG135300

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000294
Physical Location & Seq
Forward (+)
22621659 .. 22625796
4138 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG135300.1

Sequence Viewer

Length: 2592 bp
ATGGCTCAAATGTATGGAGTTTCACTCTGCATATTATTTTGCTTCCTGGGTCTCTTGTGTGCTGAAGTTGATGAAAAAGTGCATTACAACAGTCCTAGTGTTGTGGACAAGGTTGACGTTGGTGTGATTCTTGATATGGGATCAAGGGAAGCAAAACTTGTTCTCAGTTGCATTTCAATGGCCCTGTCTGATTTCTACAATCTGCACAACAACTACAGCACAAGAGTAGTTCTCCACAGTAGGGATTCCCATGGAAAAGCTCTACATGCTCTATCAGCTGCTGTTAGTCTGTTGGATAACATCAAAGTGGAAGCAATAATTAGTGCAGAACCAAGAATGGAAGCAGACCTATTGGAAGAATTAGGAGGAGTTGAAGTCCCAGTATTGTCTCTGTCTCAACCTATTCCTTCTCCTCCTCTGACTCATAGATATCCCTTCTTCATTGAGATCAGACAGGATGAAACTTCTCAGGTTATGGGAATTGTTTCCCTTATTAAAACATACAAATGGAAGGATGTTATCCTTCTATATGAGAGCACAAAATATGCACACAACATCATTCCATCTTTGATTAGTTCTTTCCAAGAGAAAAATGTCTATATTTCATATAAGAGTTCCATATCTGCCTCCTCAACAAATCAACAAATCATTGAGGAGCTCCACAAGCTCATGACATTGAAGACTAGGATAATTGTGGTGCATATTTCCCATGTGCTCATGCCTCGCCTATTCTTAAATGTAAAAAAGTTAGGAATGATGAGAGAAGGGTATGCCTGGATTATGACATCAACTAGTATGAATTTCTTGCAATCCATGGACTTATCTGTCATTGAGTCTATGCAAGGAGTGCTGGGTTTCAAGTCTCATATTCCAGCTTCAACCAACCTCCACAAACTTACTTCCAGAATCAGGAGAAAAATTTACATTGAGGAGCCTGATATGGAACTAGGTAGGGAGTTAAGTGCAGATGGAATCTGGGCATATGATGCAACTTGGGCTCTATCAGAAGCAGTTGAGAGGACAAGAATCAAAAGTTCTACTACTGGATCCTCCAAGCATGGAGTTGACATTTTTAGAGAGATTCTGCAAACTAGATTTAAAGGCTTAGGTGGTGAAGTTCGATATCCAAATGGGAACTTGATTTCAAGTGGATTTGAGATAGTGAACGTAATCAGAACAGGGGTGAGAAGAGTTGGATTTTGGCCTTATGAAGAAGAAAAAATCATGAAAGAGTCACTCCCACTTAGTAACAGGAGGAATGCACTTTCCACAAACCATCTGGAAACAATCATATGGCCTGGAGGATCATCAACCATCTCGAGAAGTTCCAAAAGGCGACTGAGTGGAATTAAACTAAAAGTTGGGGTTCCAGTAAAAATAGGGTTCAAGGAACTTGTGAGTGTGGAGCATGATGAACAAACCAATAGACCTCATGTTACAGGCTTCTGTATAGACGTATTCGAAGCTGCAATTAGAGGACTGCCTTATAAAGTACATTATGAGTTTATTCCATTTGAGAATTCCTCGGTAGTTGGGGGGTATGACGATCTGGTTTACAAGGTTTATCTACAGACATATGATGCTGTTGCTGGAGATACGACGATCACATCGAATAGATCTTTGTATGTTGATTTTACAATACCATTTACTGCCATGGGGGTGGGAATCTTAGTACGGAATGAAAAGGAAAACATGTGGGTTTTCTTGGAACCTCTGTCAGCAAATCTTTGGATGACAAGTGGTGGTTTTTTTATCCTAACGGGTTTTGTTGTGTGGATAATTGAGCGTCCTGCAAACCAAGAGTTCCAGGGATCAACATCGCAGCAACTTGGAACAATATTCTGGTTCTCCTTCTCAACCCTTGTGTTTTCTCATAGGGAAAAGTTGTTGAACAACTTGGCCAAGTTTGTCACGATCGTCTGGGTTTTTGTAGTGCTTGTATTGACATCCAGTTATACTGCAACTTTAGCATCAATGCTGACAGTTAAACAGATAAATTTAAACTCAAGAGGTAACAATATAGCTGACCTTGTGTTTTTTGGAGGGCCGAAGCCTAAGTTGTATTATACAGTCGAACAATTTGCTGATGCTTTATCAAGAGGAAGTAAGCATGGTGGTGTTTCTGCTATCATCAAAGAGATCCCATACCTCAAAATCTTCCTAGCAAAGTATCCTGCTGACTACTCCATGATTAAACACAAGTTAACTACCAATGGTTTTGGCTTTGTTTTTCCAAAAGGTTCTAAATTAGTCCGTGACATGTCGAGGCAAATTGAAATTTTAAGAGAAGAAGGAAAGCTTTTAGAGATGGAAAAGGCCTGGTTTCAGATCTCAGATGCAGTGAATAGTCCTAATCCCGGTACTCTTAACCTCAGCAGCTTTCTAGGTGTATTCCTTGTTACTGGCGTTTCTTCAGCTGCTGCTCTTATATTATTCTTCATTTTGTCCCTCAAAGATAGATGGCATGTCCTAAAGATTAGCACAGCTAGATCCCTGGGTAGAGGACAGCTGATGCAACTTATAAGAAGGTCCCTTTCTAACAAAGTATCCAATGCAGATGAACACATCAATACACAGATAGTGGATTCATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan Lig_chan-Glu_bd SBP_bac_3

Protein Analysis

863

Amino Acids

96.89

Weight (kDa)

8.97

Isoelectric Point (pI)

39.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 38 - 374 1.6e-06 Periplasmic binding protein
ANF_receptor PF01094 55 - 392 5.1e-62 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 470 - 561 4.7e-08 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 478 - 776 6.1e-11 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 574 - 803 6.6e-29 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 1488, 2522
AasI GACNNNNNNGTC 1 cut(s) 824
AclWI GGATC 7 cut(s) 148, 1041, 1054, 1312, 1819, 2134, 2484
AcoI YGGCCR 1 cut(s) 1899
AcsI RAATTY 5 cut(s) 799, 918, 1519, 1996, 2277
AcuI CTGAAG 2 cut(s) 84, 2397
AfaI GTAC 3 cut(s) 1494, 1674, 2362
AfiI CCNNNNNNNGG 3 cut(s) 241, 909, 2357
AflIII ACRYGT 2 cut(s) 1692, 2259
AgsI TTSAA 9 cut(s) 177, 374, 679, 859, 879, 1146, 1387, 1891, 2276
AhlI ACTAGT 1 cut(s) 791
AjnI CCWGG 6 cut(s) 45, 773, 1297, 1806, 2318, 2493
Alw21I GWGCWC 3 cut(s) 539, 660, 717
Alw26I GTCTC 4 cut(s) 56, 393, 399, 867
AlwI GGATC 7 cut(s) 148, 1041, 1054, 1312, 1819, 2134, 2484
AlwNI CAGNNNCTG 2 cut(s) 281, 2420
Ama87I CYCGRG 1 cut(s) 1318
AoxI GGCC 6 cut(s) 180, 1202, 1295, 1899, 2045, 2316
ApeKI GCWGC 6 cut(s) 278, 1466, 1822, 2376, 2417, 2420
ApoI RAATTY 5 cut(s) 799, 918, 1519, 1996, 2277
Asp700I GAANNNNTTC 1 cut(s) 484
AspS9I GGNCC 3 cut(s) 181, 2045, 2529
AsuC2I CCSGG 1 cut(s) 2358
AsuHPI GGTGA 2 cut(s) 1124, 1195
AsuII TTCGAA 1 cut(s) 1461
AvaI CYCGRG 1 cut(s) 1318
AvaII GGWCC 1 cut(s) 2529
BalI TGGCCA 1 cut(s) 1901
BamHI GGATCC 1 cut(s) 1046
BanII GRGCYC 2 cut(s) 660, 1000
BbsI GAAGAC 1 cut(s) 686
Bbv12I GWGCWC 3 cut(s) 539, 660, 717
BbvCI CCTCAGC 1 cut(s) 2372
BbvI GCAGC 6 cut(s) 265, 1453, 1834, 2388, 2404, 2407
BccI CCATC 6 cut(s) 571, 962, 1284, 1322, 2302, 2454
BcgI CGANNNNNNTGC 2 cut(s) 2063, 2097
BciT130I CCWGG 6 cut(s) 47, 775, 1299, 1808, 2320, 2495
BciVI GTATCC 2 cut(s) 2181, 2557
BcnI CCSGG 1 cut(s) 2358
BcoDI GTCTC 4 cut(s) 56, 393, 399, 867
BcuI ACTAGT 1 cut(s) 791
BfaI CTAG 8 cut(s) 96, 684, 792, 947, 1092, 2162, 2384, 2487
BfmI CTRYAG 2 cut(s) 214, 1568
BfuI GTATCC 2 cut(s) 2181, 2557
BglII AGATCT 2 cut(s) 1616, 2328
BisI GCNGC 6 cut(s) 279, 1467, 1823, 2377, 2418, 2421
BlsI GCNGC 6 cut(s) 280, 1468, 1824, 2378, 2419, 2422
Bme1390I CCNGG 7 cut(s) 47, 775, 1299, 1808, 2320, 2358, 2495
Bme18I GGWCC 1 cut(s) 2529
BmeT110I CYCGRG 1 cut(s) 1318
BmgT120I GGNCC 3 cut(s) 181, 2045, 2529
BmiI GGNNCC 5 cut(s) 933, 1048, 1368, 1710, 2531
BmrFI CCNGG 7 cut(s) 47, 775, 1299, 1808, 2320, 2358, 2495
BmrI ACTGGG 1 cut(s) 374
BmsI GCATC 6 cut(s) 976, 1570, 1979, 2077, 2326, 2502
BmuI ACTGGG 1 cut(s) 374
BpiI GAAGAC 1 cut(s) 686
BplI GAGNNNNNCTC 6 cut(s) 9, 41, 216, 248, 1508, 1540
BpmI CTGGAG 2 cut(s) 1320, 1611
Bpu10I CCTNAGC 2 cut(s) 1105, 2372
Bpu14I TTCGAA 1 cut(s) 1461
BpuEI CTTGAG 1 cut(s) 1990
BpuMI CCSGG 1 cut(s) 2358
BsaBI GATNNNNATC 1 cut(s) 1816
BsaI GGTCTC 1 cut(s) 56
BsaJI CCNNGG 8 cut(s) 46, 250, 813, 1524, 1653, 1807, 2493, 2494
BsaXI ACNNNNNCTCC 2 cut(s) 360, 390
Bsc4I CCNNNNNNNGG 3 cut(s) 241, 909, 2357
Bse1I ACTGG 5 cut(s) 380, 1048, 1370, 1950, 2407
Bse8I GATNNNNATC 1 cut(s) 1816
BseBI CCWGG 6 cut(s) 47, 775, 1299, 1808, 2320, 2495
BseDI CCNNGG 8 cut(s) 46, 250, 813, 1524, 1653, 1807, 2493, 2494
BseGI GGATG 4 cut(s) 463, 520, 1737, 1946
BseJI GATNNNNATC 1 cut(s) 1816
BseLI CCNNNNNNNGG 3 cut(s) 241, 909, 2357
BseMII CTCAG 5 cut(s) 178, 482, 1331, 2346, 2386
BseNI ACTGG 5 cut(s) 380, 1048, 1370, 1950, 2407
BseRI GAGGAG 6 cut(s) 381, 402, 405, 619, 668, 944
BseXI GCAGC 6 cut(s) 265, 1453, 1834, 2388, 2404, 2407
BseYI CCCAGC 1 cut(s) 850
BsgI GTGCAG 3 cut(s) 188, 345, 984
Bsh1285I CGRYCG 1 cut(s) 1917
BshFI GGCC 6 cut(s) 182, 1204, 1297, 1901, 2047, 2318
BsiEI CGRYCG 1 cut(s) 1917
BsiHKAI GWGCWC 3 cut(s) 539, 660, 717
BsiHKCI CYCGRG 1 cut(s) 1318
BsiSI CCGG 1 cut(s) 2358
BslFI GGGAC 3 cut(s) 362, 2431, 2515
BslI CCNNNNNNNGG 3 cut(s) 241, 909, 2357
BsmAI GTCTC 4 cut(s) 56, 393, 399, 867
BsmFI GGGAC 3 cut(s) 362, 2431, 2515
BsmI GAATGC 1 cut(s) 1264
BsnI GGCC 6 cut(s) 182, 1204, 1297, 1901, 2047, 2318
Bso31I GGTCTC 1 cut(s) 56
BsoBI CYCGRG 1 cut(s) 1318
Bsp119I TTCGAA 1 cut(s) 1461
Bsp1286I GDGCHC 4 cut(s) 539, 660, 717, 1000
Bsp19I CCATGG 3 cut(s) 250, 813, 1653
BspANI GGCC 6 cut(s) 182, 1204, 1297, 1901, 2047, 2318
BspCNI CTCAG 5 cut(s) 177, 481, 1332, 2345, 2385
BspHI TCATGA 2 cut(s) 669, 1224
BspLI GGNNCC 5 cut(s) 933, 1048, 1368, 1710, 2531
BspPI GGATC 7 cut(s) 148, 1041, 1054, 1312, 1819, 2134, 2484
BspT104I TTCGAA 1 cut(s) 1461
BspTNI GGTCTC 1 cut(s) 56
BsrI ACTGG 5 cut(s) 380, 1048, 1370, 1950, 2407
BssECI CCNNGG 8 cut(s) 46, 250, 813, 1524, 1653, 1807, 2493, 2494
BssT1I CCWWGG 3 cut(s) 250, 813, 1653
Bst2UI CCWGG 6 cut(s) 47, 775, 1299, 1808, 2320, 2495
Bst4CI ACNGT 4 cut(s) 92, 239, 1984, 2071
Bst6I CTCTTC 1 cut(s) 1183
BstAPI GCANNNNNTGC 2 cut(s) 847, 986
BstBI TTCGAA 1 cut(s) 1461
BstDEI CTNAG 9 cut(s) 164, 468, 1105, 1244, 1340, 1669, 2055, 2332, 2372
BstDSI CCRYGG 3 cut(s) 250, 813, 1653
BstF5I GGATG 4 cut(s) 463, 520, 1737, 1946
BstMAI GTCTC 4 cut(s) 56, 393, 399, 867
BstMCI CGRYCG 1 cut(s) 1917
BstMWI GCNNNNNNNGC 7 cut(s) 266, 275, 664, 847, 986, 995, 1967
BstNI CCWGG 6 cut(s) 47, 775, 1299, 1808, 2320, 2495
BstNSI RCATGY 4 cut(s) 269, 1696, 2263, 2468
BstSCI CCNGG 7 cut(s) 45, 773, 1297, 1806, 2318, 2356, 2493
BstSFI CTRYAG 2 cut(s) 214, 1568
BstV1I GCAGC 6 cut(s) 265, 1453, 1834, 2388, 2404, 2407
BstV2I GAAGAC 1 cut(s) 686
BstX2I RGATCY 5 cut(s) 1046, 1616, 2139, 2328, 2489
BstXI CCANNNNNNTGG 1 cut(s) 1660
BstYI RGATCY 5 cut(s) 1046, 1616, 2139, 2328, 2489
BsuI GTATCC 2 cut(s) 2181, 2557
BsuRI GGCC 6 cut(s) 182, 1204, 1297, 1901, 2047, 2318
BtgI CCRYGG 3 cut(s) 250, 813, 1653
BtgZI GCGATG 1 cut(s) 1803
BtsCI GGATG 4 cut(s) 463, 520, 1737, 1946
BtsI GCAGTG 1 cut(s) 2346
BtsIMutI CAGTG 1 cut(s) 2346
CaiI CAGNNNCTG 2 cut(s) 281, 2420
CciI TCATGA 2 cut(s) 669, 1224
Cfr13I GGNCC 3 cut(s) 181, 2045, 2529
CseI GACGC 1 cut(s) 1775
Csp6I GTAC 3 cut(s) 1493, 1673, 2361
CviQI GTAC 3 cut(s) 1493, 1673, 2361
DdeI CTNAG 9 cut(s) 164, 468, 1105, 1244, 1340, 1669, 2055, 2332, 2372
DraI TTTAAA 2 cut(s) 1099, 2001
DrdI GACNNNNNNGTC 1 cut(s) 824
DseDI GACNNNNNNGTC 1 cut(s) 824
EaeI YGGCCR 1 cut(s) 1899
Eam1104I CTCTTC 1 cut(s) 1183
EarI CTCTTC 1 cut(s) 1183
Ecl136II GAGCTC 1 cut(s) 658
Eco130I CCWWGG 3 cut(s) 250, 813, 1653
Eco147I AGGCCT 1 cut(s) 2318
Eco24I GRGCYC 2 cut(s) 660, 1000
Eco31I GGTCTC 1 cut(s) 56
Eco32I GATATC 2 cut(s) 431, 1124
Eco47I GGWCC 1 cut(s) 2529
Eco53kI GAGCTC 1 cut(s) 658
Eco57I CTGAAG 2 cut(s) 84, 2397
Eco88I CYCGRG 1 cut(s) 1318
EcoICRI GAGCTC 1 cut(s) 658
EcoO109I RGGNCCY 1 cut(s) 2529
EcoRI GAATTC 1 cut(s) 1519
EcoRII CCWGG 6 cut(s) 45, 773, 1297, 1806, 2318, 2493
EcoRV GATATC 2 cut(s) 431, 1124
EcoT14I CCWWGG 3 cut(s) 250, 813, 1653
EcoT38I GRGCYC 2 cut(s) 660, 1000
ErhI CCWWGG 3 cut(s) 250, 813, 1653
FalI AAGNNNNNCTT 4 cut(s) 141, 173, 2283, 2315
FaqI GGGAC 3 cut(s) 362, 2431, 2515
FauNDI CATATG 3 cut(s) 982, 1292, 1576
Fnu4HI GCNGC 6 cut(s) 279, 1467, 1823, 2377, 2418, 2421
FokI GGATG 4 cut(s) 470, 527, 1744, 1933
FriOI GRGCYC 2 cut(s) 660, 1000
Fsp4HI GCNGC 6 cut(s) 279, 1467, 1823, 2377, 2418, 2421
FspBI CTAG 8 cut(s) 96, 684, 792, 947, 1092, 2162, 2384, 2487
GluI GCNGC 6 cut(s) 279, 1467, 1823, 2377, 2418, 2421
GsaI CCCAGC 1 cut(s) 854
GsuI CTGGAG 2 cut(s) 1320, 1611
HaeIII GGCC 6 cut(s) 182, 1204, 1297, 1901, 2047, 2318
HapII CCGG 1 cut(s) 2358
HgaI GACGC 1 cut(s) 1775
HincII GTYRAC 3 cut(s) 115, 1066, 2205
HindII GTYRAC 3 cut(s) 115, 1066, 2205
HindIII AAGCTT 1 cut(s) 2297
HpaI GTTAAC 1 cut(s) 2205
HpaII CCGG 1 cut(s) 2358
HphI GGTGA 2 cut(s) 1124, 1195
Hpy166II GTNNAC 6 cut(s) 106, 115, 1066, 1165, 1555, 2205
Hpy188I TCNGA 7 cut(s) 190, 420, 452, 1006, 1175, 2328, 2335
Hpy8I GTNNAC 6 cut(s) 106, 115, 1066, 1165, 1555, 2205
Hpy99I CGWCG 1 cut(s) 1603
HpyAV CCTTC 8 cut(s) 417, 445, 505, 533, 758, 1861, 2285, 2520
HpyCH4III ACNGT 4 cut(s) 92, 239, 1984, 2071
HpyCH4IV ACGT 3 cut(s) 117, 1167, 1455
HpyF10VI GCNNNNNNNGC 7 cut(s) 266, 275, 664, 847, 986, 995, 1967
HpyF3I CTNAG 9 cut(s) 164, 468, 1105, 1244, 1340, 1669, 2055, 2332, 2372
HpySE526I ACGT 3 cut(s) 117, 1167, 1455
KspAI GTTAAC 1 cut(s) 2205
LmnI GCTCC 4 cut(s) 655, 663, 931, 1405
Lsp1109I GCAGC 6 cut(s) 265, 1453, 1834, 2388, 2404, 2407
LweI GCATC 6 cut(s) 976, 1570, 1979, 2077, 2326, 2502
MaeI CTAG 8 cut(s) 96, 684, 792, 947, 1092, 2162, 2384, 2487
MaeII ACGT 3 cut(s) 117, 1167, 1455
MaeIII GTNAC 7 cut(s) 1233, 1247, 1435, 1909, 2012, 2255, 2398
MflI RGATCY 5 cut(s) 1046, 1616, 2139, 2328, 2489
MhlI GDGCHC 4 cut(s) 539, 660, 717, 1000
MlsI TGGCCA 1 cut(s) 1901
MluNI TGGCCA 1 cut(s) 1901
MlyI GAGTC 3 cut(s) 415, 842, 1241
MmeI TCCRAC 2 cut(s) 273, 1174
Mox20I TGGCCA 1 cut(s) 1901
MroXI GAANNNNTTC 1 cut(s) 484
MscI TGGCCA 1 cut(s) 1901
MslI CAYNNNNRTG 5 cut(s) 176, 305, 505, 1658, 2115
Msp20I TGGCCA 1 cut(s) 1901
MspA1I CMGCKG 3 cut(s) 278, 2417, 2509
MspI CCGG 1 cut(s) 2358
MspR9I CCNGG 7 cut(s) 47, 775, 1299, 1808, 2320, 2358, 2495
Mva1269I GAATGC 1 cut(s) 1264
MvaI CCWGG 6 cut(s) 47, 775, 1299, 1808, 2320, 2495
MwoI GCNNNNNNNGC 7 cut(s) 266, 275, 664, 847, 986, 995, 1967
NciI CCSGG 1 cut(s) 2358
NcoI CCATGG 3 cut(s) 250, 813, 1653
NdeI CATATG 3 cut(s) 982, 1292, 1576
NlaIV GGNNCC 5 cut(s) 933, 1048, 1368, 1710, 2531
NmuCI GTSAC 3 cut(s) 1233, 1909, 2255
NspI RCATGY 4 cut(s) 269, 1696, 2263, 2468
NspV TTCGAA 1 cut(s) 1461
PaeR7I CTCGAG 1 cut(s) 1318
PagI TCATGA 2 cut(s) 669, 1224
PasI CCCWGGG 1 cut(s) 2494
PceI AGGCCT 1 cut(s) 2318
PciI ACATGT 2 cut(s) 1692, 2259
PcsI WCGNNNNNNNCGW 1 cut(s) 1607
PctI GAATGC 1 cut(s) 1264
PdmI GAANNNNTTC 1 cut(s) 484
PfeI GAWTC 8 cut(s) 127, 245, 906, 972, 1026, 1081, 1665, 2585
PkrI GCNGC 6 cut(s) 280, 1468, 1824, 2378, 2419, 2422
Ple19I CGATCG 1 cut(s) 1917
PleI GAGTC 3 cut(s) 415, 841, 1240
PpsI GAGTC 3 cut(s) 415, 841, 1240
PpuMI RGGWCCY 1 cut(s) 2529
PscI ACATGT 2 cut(s) 1692, 2259
PsiI TTATAA 2 cut(s) 1488, 2522
Psp124BI GAGCTC 1 cut(s) 660
Psp5II RGGWCCY 1 cut(s) 2529
Psp6I CCWGG 6 cut(s) 45, 773, 1297, 1806, 2318, 2493
PspFI CCCAGC 1 cut(s) 850
PspGI CCWGG 6 cut(s) 45, 773, 1297, 1806, 2318, 2493
PspN4I GGNNCC 5 cut(s) 933, 1048, 1368, 1710, 2531
PspPI GGNCC 3 cut(s) 181, 2045, 2529
PspPPI RGGWCCY 1 cut(s) 2529
PstNI CAGNNNCTG 2 cut(s) 281, 2420
PsuI RGATCY 5 cut(s) 1046, 1616, 2139, 2328, 2489
PvuI CGATCG 1 cut(s) 1917
PvuII CAGCTG 3 cut(s) 278, 2417, 2509
RsaI GTAC 3 cut(s) 1494, 1674, 2362
RsaNI GTAC 3 cut(s) 1493, 1673, 2361
RseI CAYNNNNRTG 5 cut(s) 176, 305, 505, 1658, 2115
SacI GAGCTC 1 cut(s) 660
SatI GCNGC 6 cut(s) 279, 1467, 1823, 2377, 2418, 2421
Sau96I GGNCC 3 cut(s) 181, 2045, 2529
SchI GAGTC 3 cut(s) 415, 842, 1241
ScrFI CCNGG 7 cut(s) 47, 775, 1299, 1808, 2320, 2358, 2495
SduI GDGCHC 4 cut(s) 539, 660, 717, 1000
SfaNI GCATC 6 cut(s) 976, 1570, 1979, 2077, 2326, 2502
SfcI CTRYAG 2 cut(s) 214, 1568
Sfr274I CTCGAG 1 cut(s) 1318
SfuI TTCGAA 1 cut(s) 1461
SinI GGWCC 1 cut(s) 2529
SlaI CTCGAG 1 cut(s) 1318
SmiMI CAYNNNNRTG 5 cut(s) 176, 305, 505, 1658, 2115
SmlI CTYRAG 2 cut(s) 1318, 2005
SmoI CTYRAG 2 cut(s) 1318, 2005
SpeI ACTAGT 1 cut(s) 791
SseBI AGGCCT 1 cut(s) 2318
SspI AATATT 1 cut(s) 1839
SspMI CTAG 8 cut(s) 96, 684, 792, 947, 1092, 2162, 2384, 2487
SstI GAGCTC 1 cut(s) 660
StuI AGGCCT 1 cut(s) 2318
StyD4I CCNGG 7 cut(s) 45, 773, 1297, 1806, 2318, 2356, 2493
StyI CCWWGG 3 cut(s) 250, 813, 1653
TaaI ACNGT 4 cut(s) 92, 239, 1984, 2071
TaiI ACGT 3 cut(s) 120, 1170, 1458
TaqI TCGA 6 cut(s) 1120, 1319, 1461, 1610, 2073, 2264
TatI WGTACW 1 cut(s) 1492
TfiI GAWTC 8 cut(s) 127, 245, 906, 972, 1026, 1081, 1665, 2585
TscAI CASTG 1 cut(s) 2346
TseFI GTSAC 3 cut(s) 1233, 1909, 2255
TseI GCWGC 6 cut(s) 278, 1466, 1822, 2376, 2417, 2420
Tsp45I GTSAC 3 cut(s) 1233, 1909, 2255
TspGWI ACGGA 2 cut(s) 1690, 2243
TspRI CASTG 1 cut(s) 2346
VpaK11BI GGWCC 1 cut(s) 2529
XapI RAATTY 5 cut(s) 799, 918, 1519, 1996, 2277
XceI RCATGY 4 cut(s) 269, 1696, 2263, 2468
XcmI CCANNNNNNNNNTGG 1 cut(s) 1276
XhoI CTCGAG 1 cut(s) 1318
XmnI GAANNNNTTC 1 cut(s) 484
XspI CTAG 8 cut(s) 96, 684, 792, 947, 1092, 2162, 2384, 2487
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.