Rroxscaffold_4G00312930

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000004
OG0000294
Physical Location & Seq
Reverse (-)
36143785 .. 36147788
4004 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_4G00312930.1

Sequence Viewer

Length: 2448 bp
ATGGAGTGTCGAGGGTTGAGGCAAGCAATCATCCTGTCCTTTGTGACCTTCTGTTGCCTCATTGGTCACTTAAGTGCTGATATTGAAAAGTTAAGCTATGATGTTGATTCTGTTATTGTTGAAGATCAGGTTCATGTAGGTGTGATTCTCGATATGGAATCAAAAGGAAAGATTGTTCTCAGCTGCATTTCAATGGCCCTCTCTGATTTTTACCATCTCCACAATAACTACAGCACAAGAATAGTTCTCTACAGTAAGGATTCCAAAGGTGAACCTCTGCCGGCTCTGTCAGCTGCTCTTGACTTTTTGGAAAATATCAAAGTGGAGGCAATAATTGGTGCACCAAAAAGAGCAGAAGCAAACCTTCTGGCAGAATTAGGAGAAGCAGCCAAAATCCCTTTAATGTCTCTTTCTCCTGCTCGGACTGACAATAAATATCCCTTCTTTGTTGAGATCATGCGCGCTGATGAAGCGGCTGAAGTTAAGGGAATTAGTGCCATTATTGAAGTTTTCAAATGGAGGGATGTCATCCTTTTATATGATAGCAAAGAATATGAGAGAGACTTCATTCCATCATTAGTCAAGTCATTCCAAGAGAGCACACATGGGAGTGTGGCGTACAAAAGTTCCAATATTGCTTCCTCCTCATCAAATGAAGAGATCACTGCAGAGCTCCAAAAGTTGATCAACCTGAAGATTAAGGTATTTGTGGTGCATGTGTCACATTTTTTTGCACCTCGTCTTTTCTTGAATGCAAAGAAGTTAGGGATGATGAGCGAAGGGTATGCTTGGATCATGACATCAACTAGCATGAATTTCTTGCATTCTATGAATTTATCTGTTATTGAGTCAATGCAAGGAGTGCTGGGTTTCAAGTCTTCTATTCCAGCATCAATAAACCTTCACAGTCTTACATCAAGATTGAGGAGGAAGTTTTACATGGAGGATCCAAATATGGAAGCAATTCGGGAGTTGAGTGCAGATGGGATCTGGGCATATGATGCAACTTGGGCTCTAGCAGAAGCAGTTGAAAGAGCAAGGCTTAAAAATTCTACCACTAGATCCTCCAAAGATGGAGCTGTGCTTCTTAGAGAGATATTGCAGACCAGATTCAAAGGTTTAAGTGGTGAAATCCAATATGTGAATGGGAAACTGATTCCATCAGAACCATTTGAGATAGTTAATGTTATTGGAAAAGGTGAGATAAAAAAGATTGGATTTTGGCCTTGCAAAGAAGAAGTAAAATCCGGAAAATGGTCACCCCAGCAGCAACTCATTCATAATGGGAGAAATTTAGCTTCTGCAATTGATCTTAAAAGAATCATATGGCCCGGAGGTTCCAGGAGGGAATTGAGTAGTAGCGAAACACTTGAACTAAAAATTGGTGTTCCAGTGAGAATTGGGTTCAAGGAACTTGTTCGTGTTGAGCATGATCTTGAAACCAATACAACTCATGTCACTGGCTTCTCTATAGATGTATTCAAAGCTGCAATAGGAGCTTTGCCGTATGAAGTGCCATACGAGTTTATTCCATTTGAGGATGCCAATGGAAATCCTGCAGGGACTTATAATGATCTTGTTTACCAGGTTTATCTCAAGAAATACGATGCTGTTGTTGGAGATGTTACGATCACAGAGAACAGATCTCAGTATGTTGATTTTACAACACCATATACCGACTTAGCGGAAAAGTTGTCAAATAATTTGGCAAAGTTTGTAGTGATCATATGGGTGTTTGTAGTGCTTATATTGTCATCAAGTTATACTGCAACTTTAGCATCAATGATGACGGTCAAACAGATAGAGTTAAACTCGAGAGGGAACTATATAGGTTATCAATCGGGTTCATTAGGAGTTCTAGTGAACTTAAATTTGAAAGCAGCGAAGTCATATCGTTCAGAAGAAGAATATATTGATGCTCTATCCAAAGGAAGCAAGAATGGTGGAGTTTCTGGGATTATTGACGAGGTTCCGTACATCAATATCTTCCTTGCAAAGTATTCTGCTGACTACTCGATGATTAAAACCAGGTCTATCACCAATGGTTTTGCCTTTGTTTTCCCTAAAGGTTCGAAATTGGTTCACGATGTGTCAAGGCAAATTGAACGTCTGAGACAGGAAGAAAAGCTTCTAGAGATGGAAAAGACCTGGTTTCGTGAAAAAACAAGTCTCATGTCTGATGAGGATATGACCCAGAGTGATCCCAATACGATTGACCTCTATGACTTTCGTGGTCTGTTCCTTATTAGTGGGGCTTCTTTAGCTATTTCTCTTTTCTTCTTCGTAGTTTTATCTACTAGATTTAGGAATCTGTTTCAAGGACTAATGCAGCTGATTCGTATAGAACTGCAACGTTTGAGAACTTTCTTCTCCTTCATAGTATTCAATGGAATTGAGCAAAACTGCATGCAAGTTCCTACGCAAGTCATTGTACATGCTGTCCATTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

815

Amino Acids

91.64

Weight (kDa)

6.37

Isoelectric Point (pI)

37.11

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 44 - 396 2e-15 Periplasmic binding protein
ANF_receptor PF01094 61 - 397 1.8e-60 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 477 - 561 3.5e-08 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 482 - 719 2.5e-10 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 563 - 752 3e-12 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1569
AccII CGCG 1 cut(s) 462
AccIII TCCGGA 1 cut(s) 1247
AciI CCGC 2 cut(s) 473, 1685
AclI AACGTT 1 cut(s) 2353
AclWI GGATC 6 cut(s) 800, 941, 954, 995, 1056, 2195
AcsI RAATTY 5 cut(s) 814, 832, 1048, 1291, 1870
AcuI CTGAAG 2 cut(s) 498, 713
AdeI CACNNNGTG 1 cut(s) 2089
AfaI GTAC 3 cut(s) 620, 1976, 2433
AfiI CCNNNNNNNGG 1 cut(s) 1254
AflII CTTAAG 1 cut(s) 70
AjnI CCWGG 4 cut(s) 1340, 1584, 2027, 2147
AjuI GAANNNNNNNTTGG 2 cut(s) 1365, 1397
AleI CACNNNNGTG 1 cut(s) 72
AloI GAACNNNNNNTCC 2 cut(s) 159, 191
Alw21I GWGCWC 3 cut(s) 343, 602, 675
Alw26I GTCTC 4 cut(s) 411, 555, 2107, 2174
Alw44I GTGCAC 1 cut(s) 339
AlwI GGATC 6 cut(s) 800, 941, 954, 995, 1056, 2195
Ama87I CYCGRG 1 cut(s) 1813
Aor13HI TCCGGA 1 cut(s) 1247
AoxI GGCC 3 cut(s) 195, 1223, 1328
ApaLI GTGCAC 1 cut(s) 339
ApeKI GCWGC 7 cut(s) 183, 293, 386, 1267, 1487, 1880, 2329
ApoI RAATTY 5 cut(s) 814, 832, 1048, 1291, 1870
ArsI GACNNNNNNTTYG 2 cut(s) 2092, 2124
Asp700I GAANNNNTTC 3 cut(s) 963, 1416, 2127
AspLEI GCGC 2 cut(s) 462, 464
AspS9I GGNCC 2 cut(s) 196, 1329
AsuC2I CCSGG 1 cut(s) 1332
AsuHPI GGTGA 5 cut(s) 281, 1139, 1211, 1251, 2029
AsuII TTCGAA 1 cut(s) 2072
AvaI CYCGRG 1 cut(s) 1813
BaeGI GKGCMC 1 cut(s) 343
BamHI GGATCC 1 cut(s) 946
BanII GRGCYC 2 cut(s) 675, 1015
BbsI GAAGAC 1 cut(s) 870
Bbv12I GWGCWC 3 cut(s) 343, 602, 675
BbvI GCAGC 7 cut(s) 170, 280, 398, 1279, 1474, 1892, 2341
BccI CCATC 6 cut(s) 222, 580, 977, 1067, 1168, 2131
BceAI ACGGC 1 cut(s) 1489
BcgI CGANNNNNNTGC 4 cut(s) 767, 801, 2318, 2352
BciT130I CCWGG 4 cut(s) 1342, 1586, 2029, 2149
BclI TGATCA 2 cut(s) 684, 1722
BcnI CCSGG 1 cut(s) 1332
BcoDI GTCTC 4 cut(s) 411, 555, 2107, 2174
BfaI CTAG 6 cut(s) 807, 1016, 1059, 1859, 2132, 2298
BfmI CTRYAG 5 cut(s) 229, 250, 666, 1470, 1557
BfrI CTTAAG 1 cut(s) 70
BglII AGATCT 1 cut(s) 1643
BisI GCNGC 8 cut(s) 184, 294, 387, 474, 1268, 1488, 1881, 2330
BlsI GCNGC 8 cut(s) 185, 295, 388, 475, 1269, 1489, 1882, 2331
Bme1390I CCNGG 5 cut(s) 1332, 1342, 1586, 2029, 2149
BmeT110I CYCGRG 1 cut(s) 1813
BmgT120I GGNCC 2 cut(s) 196, 1329
BmiI GGNNCC 3 cut(s) 948, 1339, 1971
BmrFI CCNGG 5 cut(s) 1332, 1342, 1586, 2029, 2149
BmsI GCATC 6 cut(s) 899, 991, 1531, 1597, 1787, 1906
BpiI GAAGAC 1 cut(s) 870
BplI GAGNNNNNCTC 2 cut(s) 1796, 1828
Bpu14I TTCGAA 1 cut(s) 2072
BpuEI CTTGAG 1 cut(s) 1580
BpuMI CCSGG 1 cut(s) 1332
BsaWI WCCGGW 1 cut(s) 1247
BsaXI ACNNNNNCTCC 2 cut(s) 1845, 1875
Bsc4I CCNNNNNNNGG 1 cut(s) 1254
Bse118I RCCGGY 1 cut(s) 280
Bse1I ACTGG 2 cut(s) 1391, 1465
BseAI TCCGGA 1 cut(s) 1247
BseBI CCWGG 4 cut(s) 1342, 1586, 2029, 2149
BseGI GGATG 5 cut(s) 30, 528, 529, 774, 1546
BseLI CCNNNNNNNGG 1 cut(s) 1254
BseMII CTCAG 3 cut(s) 193, 1661, 2102
BseNI ACTGG 2 cut(s) 1391, 1465
BsePI GCGCGC 1 cut(s) 460
BseRI GAGGAG 2 cut(s) 634, 940
BseSI GKGCMC 1 cut(s) 343
BseXI GCAGC 7 cut(s) 170, 280, 398, 1279, 1474, 1892, 2341
BseYI CCCAGC 2 cut(s) 865, 1263
BsgI GTGCAG 1 cut(s) 999
Bsh1236I CGCG 1 cut(s) 462
BshFI GGCC 3 cut(s) 197, 1225, 1330
BsiHKAI GWGCWC 3 cut(s) 343, 602, 675
BsiHKCI CYCGRG 1 cut(s) 1813
BsiSI CCGG 3 cut(s) 281, 1248, 1332
BslFI GGGAC 1 cut(s) 1576
BslI CCNNNNNNNGG 1 cut(s) 1254
BsmAI GTCTC 4 cut(s) 411, 555, 2107, 2174
BsmFI GGGAC 1 cut(s) 1576
BsmI GAATGC 2 cut(s) 757, 823
BsnI GGCC 3 cut(s) 197, 1225, 1330
BsoBI CYCGRG 1 cut(s) 1813
Bsp119I TTCGAA 1 cut(s) 2072
Bsp1286I GDGCHC 4 cut(s) 343, 602, 675, 1015
Bsp13I TCCGGA 1 cut(s) 1247
Bsp1407I TGTACA 1 cut(s) 2431
BspACI CCGC 2 cut(s) 473, 1685
BspANI GGCC 3 cut(s) 197, 1225, 1330
BspCNI CTCAG 3 cut(s) 192, 1660, 2103
BspEI TCCGGA 1 cut(s) 1247
BspFNI CGCG 1 cut(s) 462
BspHI TCATGA 1 cut(s) 795
BspLI GGNNCC 3 cut(s) 948, 1339, 1971
BspMAI CTGCAG 2 cut(s) 670, 1561
BspPI GGATC 6 cut(s) 800, 941, 954, 995, 1056, 2195
BspT104I TTCGAA 1 cut(s) 2072
BspTI CTTAAG 1 cut(s) 70
BsrFI RCCGGY 1 cut(s) 280
BsrGI TGTACA 1 cut(s) 2431
BsrI ACTGG 2 cut(s) 1391, 1465
BssAI RCCGGY 1 cut(s) 280
BssHII GCGCGC 1 cut(s) 460
Bst2UI CCWGG 4 cut(s) 1342, 1586, 2029, 2149
Bst4CI ACNGT 3 cut(s) 254, 908, 1792
Bst6I CTCTTC 1 cut(s) 651
BstAFI CTTAAG 1 cut(s) 70
BstAPI GCANNNNNTGC 2 cut(s) 862, 1001
BstAUI TGTACA 1 cut(s) 2431
BstBI TTCGAA 1 cut(s) 2072
BstC8I GCNNGC 4 cut(s) 24, 282, 462, 2408
BstDEI CTNAG 5 cut(s) 179, 1088, 1647, 1681, 2111
BstEII GGTNACC 1 cut(s) 1257
BstF5I GGATG 5 cut(s) 30, 528, 529, 774, 1546
BstFNI CGCG 1 cut(s) 462
BstHHI GCGC 2 cut(s) 462, 464
BstMAI GTCTC 4 cut(s) 411, 555, 2107, 2174
BstMWI GCNNNNNNNGC 8 cut(s) 290, 470, 862, 1001, 1010, 1496, 1775, 2261
BstNI CCWGG 4 cut(s) 1342, 1586, 2029, 2149
BstNSI RCATGY 3 cut(s) 719, 2410, 2438
BstPI GGTNACC 1 cut(s) 1257
BstSCI CCNGG 5 cut(s) 1330, 1340, 1584, 2027, 2147
BstSFI CTRYAG 5 cut(s) 229, 250, 666, 1470, 1557
BstSLI GKGCMC 1 cut(s) 343
BstUI CGCG 1 cut(s) 462
BstV1I GCAGC 7 cut(s) 170, 280, 398, 1279, 1474, 1892, 2341
BstV2I GAAGAC 1 cut(s) 870
BstX2I RGATCY 4 cut(s) 946, 987, 1061, 1643
BstYI RGATCY 4 cut(s) 946, 987, 1061, 1643
BsuRI GGCC 3 cut(s) 197, 1225, 1330
BtsCI GGATG 5 cut(s) 30, 528, 529, 774, 1546
BtsI GCAGTG 1 cut(s) 663
BtsIMutI CAGTG 3 cut(s) 663, 1398, 1458
Cac8I GCNNGC 4 cut(s) 24, 282, 462, 2408
CciI TCATGA 1 cut(s) 795
CfoI GCGC 2 cut(s) 462, 464
Cfr10I RCCGGY 1 cut(s) 280
Cfr13I GGNCC 2 cut(s) 196, 1329
CsiI ACCWGGT 3 cut(s) 1584, 2027, 2147
Csp6I GTAC 3 cut(s) 619, 1975, 2432
CspCI CAANNNNNGTGG 2 cut(s) 1924, 1959
CviQI GTAC 3 cut(s) 619, 1975, 2432
DdeI CTNAG 5 cut(s) 179, 1088, 1647, 1681, 2111
DraIII CACNNNGTG 1 cut(s) 2089
Eam1104I CTCTTC 1 cut(s) 651
EarI CTCTTC 1 cut(s) 651
Ecl136II GAGCTC 1 cut(s) 673
Eco24I GRGCYC 2 cut(s) 675, 1015
Eco53kI GAGCTC 1 cut(s) 673
Eco57I CTGAAG 2 cut(s) 498, 713
Eco88I CYCGRG 1 cut(s) 1813
Eco91I GGTNACC 1 cut(s) 1257
EcoICRI GAGCTC 1 cut(s) 673
EcoO65I GGTNACC 1 cut(s) 1257
EcoRII CCWGG 4 cut(s) 1340, 1584, 2027, 2147
EcoT38I GRGCYC 2 cut(s) 675, 1015
FalI AAGNNNNNCTT 4 cut(s) 348, 380, 2112, 2144
FaqI GGGAC 1 cut(s) 1576
FauNDI CATATG 3 cut(s) 997, 1325, 1727
FbaI TGATCA 2 cut(s) 684, 1722
Fnu4HI GCNGC 8 cut(s) 184, 294, 387, 474, 1268, 1488, 1881, 2330
FokI GGATG 5 cut(s) 17, 515, 536, 781, 1553
FriOI GRGCYC 2 cut(s) 675, 1015
Fsp4HI GCNGC 8 cut(s) 184, 294, 387, 474, 1268, 1488, 1881, 2330
FspBI CTAG 6 cut(s) 807, 1016, 1059, 1859, 2132, 2298
GlaI GCGC 2 cut(s) 461, 463
GluI GCNGC 8 cut(s) 184, 294, 387, 474, 1268, 1488, 1881, 2330
GsaI CCCAGC 2 cut(s) 869, 1267
HaeIII GGCC 3 cut(s) 197, 1225, 1330
HapII CCGG 3 cut(s) 281, 1248, 1332
HhaI GCGC 2 cut(s) 462, 464
Hin6I GCGC 2 cut(s) 460, 462
HinP1I GCGC 2 cut(s) 460, 462
HindIII AAGCTT 1 cut(s) 2126
HpaII CCGG 3 cut(s) 281, 1248, 1332
HphI GGTGA 5 cut(s) 281, 1139, 1211, 1251, 2029
Hpy166II GTNNAC 5 cut(s) 272, 341, 1582, 1864, 2083
Hpy188I TCNGA 6 cut(s) 205, 423, 1165, 1900, 2112, 2179
Hpy8I GTNNAC 5 cut(s) 272, 341, 1582, 1864, 2083
HpyAV CCTTC 6 cut(s) 58, 374, 451, 773, 911, 2383
HpyCH4III ACNGT 3 cut(s) 254, 908, 1792
HpyCH4IV ACGT 2 cut(s) 2107, 2353
HpyF10VI GCNNNNNNNGC 8 cut(s) 290, 470, 862, 1001, 1010, 1496, 1775, 2261
HpyF3I CTNAG 5 cut(s) 179, 1088, 1647, 1681, 2111
HpySE526I ACGT 2 cut(s) 2107, 2353
HspAI GCGC 2 cut(s) 460, 462
Kpn2I TCCGGA 1 cut(s) 1247
KroI GCCGGC 1 cut(s) 280
KroNI GCCGGC 1 cut(s) 282
Ksp22I TGATCA 2 cut(s) 684, 1722
LmnI GCTCC 3 cut(s) 678, 1076, 1496
Lsp1109I GCAGC 7 cut(s) 170, 280, 398, 1279, 1474, 1892, 2341
LweI GCATC 6 cut(s) 899, 991, 1531, 1597, 1787, 1906
MabI ACCWGGT 3 cut(s) 1584, 2027, 2147
MaeI CTAG 6 cut(s) 807, 1016, 1059, 1859, 2132, 2298
MaeII ACGT 2 cut(s) 2107, 2353
MaeIII GTNAC 6 cut(s) 43, 65, 720, 1257, 1456, 1624
MfeI CAATTG 1 cut(s) 1305
MflI RGATCY 4 cut(s) 946, 987, 1061, 1643
MhlI GDGCHC 4 cut(s) 343, 602, 675, 1015
MlyI GAGTC 1 cut(s) 857
MmeI TCCRAC 1 cut(s) 1597
MroI TCCGGA 1 cut(s) 1247
MroNI GCCGGC 1 cut(s) 280
MroXI GAANNNNTTC 3 cut(s) 963, 1416, 2127
MslI CAYNNNNRTG 5 cut(s) 72, 138, 191, 609, 1730
MspA1I CMGCKG 3 cut(s) 183, 293, 2332
MspCI CTTAAG 1 cut(s) 70
MspI CCGG 3 cut(s) 281, 1248, 1332
MspR9I CCNGG 5 cut(s) 1332, 1342, 1586, 2029, 2149
MunI CAATTG 1 cut(s) 1305
Mva1269I GAATGC 2 cut(s) 757, 823
MvaI CCWGG 4 cut(s) 1342, 1586, 2029, 2149
MvnI CGCG 1 cut(s) 462
MwoI GCNNNNNNNGC 8 cut(s) 290, 470, 862, 1001, 1010, 1496, 1775, 2261
NaeI GCCGGC 1 cut(s) 282
NciI CCSGG 1 cut(s) 1332
NdeI CATATG 3 cut(s) 997, 1325, 1727
NgoMIV GCCGGC 1 cut(s) 280
NlaIV GGNNCC 3 cut(s) 948, 1339, 1971
NmuCI GTSAC 5 cut(s) 43, 65, 720, 1257, 1456
NspI RCATGY 3 cut(s) 719, 2410, 2438
NspV TTCGAA 1 cut(s) 2072
OliI CACNNNNGTG 1 cut(s) 72
PaeI GCATGC 1 cut(s) 2410
PaeR7I CTCGAG 1 cut(s) 1813
PagI TCATGA 1 cut(s) 795
PauI GCGCGC 1 cut(s) 460
PctI GAATGC 2 cut(s) 757, 823
PdiI GCCGGC 1 cut(s) 282
PdmI GAANNNNTTC 3 cut(s) 963, 1416, 2127
PfeI GAWTC 9 cut(s) 107, 145, 158, 260, 1110, 1156, 1320, 2308, 2335
PfoI TCCNGGA 1 cut(s) 1340
PkrI GCNGC 8 cut(s) 185, 295, 388, 475, 1269, 1489, 1882, 2331
PleI GAGTC 1 cut(s) 856
PpsI GAGTC 1 cut(s) 856
PsiI TTATAA 1 cut(s) 1569
Psp124BI GAGCTC 1 cut(s) 675
Psp1406I AACGTT 1 cut(s) 2353
Psp6I CCWGG 4 cut(s) 1340, 1584, 2027, 2147
PspEI GGTNACC 1 cut(s) 1257
PspFI CCCAGC 2 cut(s) 865, 1263
PspGI CCWGG 4 cut(s) 1340, 1584, 2027, 2147
PspN4I GGNNCC 3 cut(s) 948, 1339, 1971
PspPI GGNCC 2 cut(s) 196, 1329
PstI CTGCAG 2 cut(s) 670, 1561
PsuI RGATCY 4 cut(s) 946, 987, 1061, 1643
PteI GCGCGC 1 cut(s) 460
PvuII CAGCTG 3 cut(s) 183, 293, 2332
RsaI GTAC 3 cut(s) 620, 1976, 2433
RsaNI GTAC 3 cut(s) 619, 1975, 2432
RseI CAYNNNNRTG 5 cut(s) 72, 138, 191, 609, 1730
SacI GAGCTC 1 cut(s) 675
SatI GCNGC 8 cut(s) 184, 294, 387, 474, 1268, 1488, 1881, 2330
Sau96I GGNCC 2 cut(s) 196, 1329
SbfI CCTGCAGG 1 cut(s) 1561
SchI GAGTC 1 cut(s) 857
ScrFI CCNGG 5 cut(s) 1332, 1342, 1586, 2029, 2149
SdaI CCTGCAGG 1 cut(s) 1561
SduI GDGCHC 4 cut(s) 343, 602, 675, 1015
SexAI ACCWGGT 3 cut(s) 1584, 2027, 2147
SfaNI GCATC 6 cut(s) 899, 991, 1531, 1597, 1787, 1906
SfcI CTRYAG 5 cut(s) 229, 250, 666, 1470, 1557
Sfr274I CTCGAG 1 cut(s) 1813
SfuI TTCGAA 1 cut(s) 2072
SlaI CTCGAG 1 cut(s) 1813
SmiMI CAYNNNNRTG 5 cut(s) 72, 138, 191, 609, 1730
SmlI CTYRAG 3 cut(s) 70, 1595, 1813
SmoI CTYRAG 3 cut(s) 70, 1595, 1813
SphI GCATGC 1 cut(s) 2410
Sse8387I CCTGCAGG 1 cut(s) 1561
SsiI CCGC 2 cut(s) 473, 1685
SspI AATATT 1 cut(s) 634
SspMI CTAG 6 cut(s) 807, 1016, 1059, 1859, 2132, 2298
SstI GAGCTC 1 cut(s) 675
StyD4I CCNGG 5 cut(s) 1330, 1340, 1584, 2027, 2147
TaaI ACNGT 3 cut(s) 254, 908, 1792
TaiI ACGT 2 cut(s) 2110, 2356
TaqI TCGA 5 cut(s) 10, 150, 1814, 2015, 2072
TatI WGTACW 1 cut(s) 2431
TauI GCSGC 1 cut(s) 476
TfiI GAWTC 9 cut(s) 107, 145, 158, 260, 1110, 1156, 1320, 2308, 2335
TscAI CASTG 3 cut(s) 670, 1398, 1465
TseFI GTSAC 5 cut(s) 43, 65, 720, 1257, 1456
TseI GCWGC 7 cut(s) 183, 293, 386, 1267, 1487, 1880, 2329
Tsp45I GTSAC 5 cut(s) 43, 65, 720, 1257, 1456
TspGWI ACGGA 1 cut(s) 1962
TspRI CASTG 3 cut(s) 670, 1398, 1465
Vha464I CTTAAG 1 cut(s) 70
VneI GTGCAC 1 cut(s) 339
XapI RAATTY 5 cut(s) 814, 832, 1048, 1291, 1870
XbaI TCTAGA 1 cut(s) 2131
XceI RCATGY 3 cut(s) 719, 2410, 2438
XcmI CCANNNNNNNNNTGG 1 cut(s) 1142
XhoI CTCGAG 1 cut(s) 1813
XmnI GAANNNNTTC 3 cut(s) 963, 1416, 2127
XspI CTAG 6 cut(s) 807, 1016, 1059, 1859, 2132, 2298
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.