Rh1BG134800

Glutamate receptor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr1B
OG0000294
Physical Location & Seq
Forward (+)
22583780 .. 22587328
3549 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh1BG134800.1

Sequence Viewer

Length: 2307 bp
ATGGAGTTTCAAGAGTTGAGGCAAACAGTCATCCTGGCCTTTGTGACTTTCTGTTGCCTCATTGGTCACTTGTGTGCTGATATTGAAAAAACAAATAGTGATACTAGTCCTGTTGTTGTTGAAGATCAGGTTCATGTCGGTGTCATTCTCGATATGGGATCAAGGGAAGGAAAGATTATTCTCAGCTGCATATCAATGGCCCTCTCTGATTTTTACCATCTGCATGATAACTACAGTACAAGAGTAGTTCTCCACAGTAAGGATTCCAAAGGTGAACCTCTGCCTGCTCTGTCAGCTGCCCTTGGTCTTTTGGAAAACATCAAAGTGGAATCAATAATTGGTGCACAAACAAGAGCGGAAGCAAACCTTTTGGCAGAATTAGGAGAAGCGGCTAAACTCCCTGTAATGTCTCTTTCTCCTCCTCTGACCAACAATAACTATCCTTTCTTTGTTGAGATCATGCGCGGTGATCAAGCTGCTGAAGTTAGGGGAATCAGTGCGCTTATTGAAGTATTCAAATGGAGGGATGTTATACTTTTATATGATAATAAAGAATATGAGAAAGACTTCATTCCATCATTAGTCAACTCTTTCCAAGAGATCACACATGGGAGTGTTTCATACCAGAATTCCAATATTGCTTCCTCCTCATCAAATGAAGAAATCATCGAAGAGCTCCAAAAGCTGATGAGATTGAAGATTAAGGTATTTGTGGTGCATGTGTCACATATGCTTGCACCACGCCTTTTCTTAATAGCAAATAAGTTAGGGATGATGAGTGAAGGGTATGCTTGGATTGTGACTTCGAGTAGCATGAATTTATTGCAATCCATGAATTTATCTGTTATCGAGTCAATGCAAGGAGTGCTGGGTTTCAAGTCTTTTATTCCAGCATCAATGAGCCTTCACAGTCTTACTTCAAGACTGAGGACGAAGTTTTACCTGGAGGATCCCAATATGGAAGCAATTCGGGAGTTAAGTGCAGATGGGATCTGGGCATATGATACAACTTGGGCTCTAGCAGAAGCAGTTCAAAGAGCAAGGCTTAAAAATTCTACAACTAGATCCTCCAAAGATGGAGCTGTGCTTCTTAGAGAGATATTGCAGACTAGATTAAAAGGTTTAAGTGGTGAAATTCAATATGTAAATGGGAAATTGATTCCATCAAAACCATTTGAGATAGTTAATGTTATTGGAAAAGGTGAGATAAAAAGGATTGGATTTTGGCCTTGCAAAGAAGAAGTAAAATCCGGAAAATGGTTAACCCATCAGCAGCTTATTCATGGTAGGAGAAATTTAGCTTCTACAATTGATCTTGAAAGAATCATCTGGCCTGGAGGATCCAAGAGGGACTTGAGCAGTAGTGAAACACTTAAACTAAAAATTGGTGTTCCAGTGAGATATGGGTTCAAGGAACTTGTTCGTGTCGAGCATGATCTTGAAACCAATGCAACTCATGTCACTGGCTTCTCAATAGATGTATTCAAAGCTGCAGTAGGAGCTTTGCCGTATGAAGTGCAGTACGAGTTTATTCCATTTGAGGATGCCAATGGAAATCCTGCAGGGACTAATAATGATCTTGTTTACCAGGTTTATCTCAAGAAATTCGATGCTGTTGTTGGAGATGTTACGATCACAGAGAACAGATCTCAGTATGTTGATTTTACAATCCCATATACTGACTTAGGTGTGGGAATGTTGGTGCCCAATGCCAAGGAAAACATGTGGATTTTCTTTAAACCACTTTCAACATATCTTTGGATAACAACTGCTGCTTTCTTTATCCTTACCGGTTTTGTTGTCTGGATATTTGAGCATCCTACTAATGAAAACTTCCAAGGCACAGCAGCGGAACAAATTGGAACTGTATTCTGGTTCTCCTTCTCTACTCTTGTGTTCACTTATACGGAGAAGTTATCAAATAATTTGACAAAGTTTGTAGTGATCATATGGGTGTTTTTAGTGCTTATATTGACGTCGACTTACACTGCAACTTTAGCATCAATGATGACTGTGAAACAGATACAGTTAAACTCAAGAGGAAACGATATAGGTTACCAATCGGGTTCCTTAGGGGTTATAGTGAACTTGAATTTCAAAGGGCTTAAGCCATATCGTTCAAGTGAGGAGTATTTTGTTGCTTTATCAAAAGGAAGCAAGCATAATGGTGTTTCTGGGGTTATTGACGAGGTTCCATACATCAACATCTTCCTCGCAAACTATTCTGCTGAGTACTCGATGATTAAAACCAAGTCTATCACCAATGGTTTTGCCTTTGTGAGTAACTCTCCCCTACTAGTCACTTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ANF_receptor Lig_chan SBP_bac_3

Protein Analysis

768

Amino Acids

85.86

Weight (kDa)

6.07

Isoelectric Point (pI)

31.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peripla_BP_6 PF13458 44 - 383 3.1e-10 Periplasmic binding protein
ANF_receptor PF01094 63 - 399 1.5e-56 Receptor family ligand binding region
Lig_chan-Glu_bd PF10613 477 - 570 2.2e-10 Ligated ion channel L-glutamate- and glycine-binding site
SBP_bac_3 PF00497 484 - 622 2.6e-08 Bacterial extracellular solute-binding proteins, family 3
Lig_chan PF00060 584 - 677 7.2e-22 Ligand-gated ion channel
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Orthologous Genes (Group: OG0000294)

Species Orthologous Gene IDs
arabidopsis_thaliana AT3G07520 AT5G48400 AT5G48400 AT5G48410 AT5G48410 AT5G48410 AT5G48410
fragaria_vesca FvH4_1g26430 FvH4_7g07770 FvH4_7g07820 FvH4_7g07830 FvH4_7g07830
malus_domestica MD02G1232900.v1.1 MD02G1233100.v1.1 MD07G1080800.v1.1
prunus_persica Prupe.2G105100_v2.0.a1 Prupe.2G105200_v2.0.a1 Prupe.2G105300_v2.0.a1 Prupe.2G105400_v2.0.a1 Prupe.2G105500_v2.0.a1 Prupe.2G105600_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105700_v2.0.a1 Prupe.2G105800_v2.0.a1
pyrus_communis pycom02g20100 pycom07g06420
rosa_chinensis RchiOBHm_Chr1g0341721 RchiOBHm_Chr1g0341731 RchiOBHm_Chr1g0341751 RchiOBHm_Chr1g0341761 RchiOBHm_Chr1g0341831 RchiOBHm_Chr1g0341851 RchiOBHm_Chr1g0341911 RchiOBHm_Chr1g0342001 RchiOBHm_Chr1g0342301 RchiOBHm_Chr5g0063031 RchiOBHm_Chr5g0063061
rosa_laevigata RLG00000029107 RLG00000029109 RLG00000029110 RLG00000029111 RLG00000029123 RLG00000029125 RLG00000029131 RLG00000029136 RLG00000029150 RLG00000029650 RLG00000029651 RLG00000035598
rosa_multiflora Rmu_sc0000696.1_g000008 Rmu_sc0001055.1_g000009 Rmu_sc0001055.1_g000014 Rmu_sc0001547.1_g000003 Rmu_sc0002161.1_g000003 Rmu_sc0002302.1_g000023 Rmu_sc0002322.1_g000082 Rmu_sc0004658.1_g000002 Rmu_sc0005331.1_g000003 Rmu_sc0007655.1_g000001 Rmu_sc0007655.1_g000003 Rmu_sc0018911.1_g000004 Rmu_sc0018911.1_g000011 Rmu_sc0034500.1_g000001
rosa_roxburghii Rroxscaffold_4G00312490 Rroxscaffold_4G00312540 Rroxscaffold_4G00312660 Rroxscaffold_4G00312720 Rroxscaffold_4G00312820 Rroxscaffold_4G00312930
rosa_rugosa Rorug01G0151800.1 Rorug01G0151900.1 Rorug01G0152000.1 Rorug01G0154000.1 Rorug01G0154100.1 Rorug05G0353600 Rorug05G0353700 Rorug05G0353800
rosa_samantha Rh1AG166600 Rh1AG168100 Rh1AG169100 Rh1AG169200 Rh1AG169600 Rh1AG169800 Rh1BG132800 Rh1BG132900 Rh1BG133600 Rh1BG133700 Rh1BG134800 Rh1BG135300 Rh1BG135500 Rh1BG135900 Rh1BG136000 Rh1BG136100 Rh1BG136400 Rh1BG137300 Rh1BG137600 Rh1BG137800 Rh1CG155000 Rh1CG155600 Rh1CG156900 Rh1CG157500 Rh1CG157800 Rh1CG158100 Rh1DG167700 Rh1DG168200 Rh1DG168900 Rh1DG169000 Rh1DG169500 Rh5AG414400 Rh5BG429200 Rh5CG452600 Rh5DG379600 Rh5DG442600 Rh5DG442700
rosa_wichuraiana Rw0G014260 Rw0G017550 Rw1G013900 Rw1G014090 Rw1G014110 Rw1G014130 Rw1G014150 Rw5G038950

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 1979
AccB1I GGYRCC 1 cut(s) 1702
AccBSI CCGCTC 1 cut(s) 356
AccI GTMKAC 1 cut(s) 1979
AccII CGCG 1 cut(s) 465
AccIII TCCGGA 1 cut(s) 1250
AciI CCGC 4 cut(s) 356, 389, 465, 1850
AclWI GGATC 7 cut(s) 166, 944, 957, 998, 1059, 1335, 1348
AcsI RAATTY 8 cut(s) 628, 817, 835, 1051, 1134, 1294, 1604, 2092
AcuI CTGAAG 1 cut(s) 501
AcyI GRCGYC 1 cut(s) 1976
AfaI GTAC 3 cut(s) 238, 1523, 2234
AfiI CCNNNNNNNGG 2 cut(s) 259, 1257
AflII CTTAAG 1 cut(s) 2105
AflIII ACRYGT 1 cut(s) 1722
AgeI ACCGGT 1 cut(s) 1790
AhlI ACTAGT 2 cut(s) 104, 2296
AjnI CCWGG 4 cut(s) 33, 942, 1333, 1587
AjuI GAANNNNNNNTTGG 2 cut(s) 321, 353
AleI CACNNNNGTG 1 cut(s) 72
Alw21I GWGCWC 2 cut(s) 346, 678
Alw26I GTCTC 1 cut(s) 414
Alw44I GTGCAC 1 cut(s) 342
AlwI GGATC 7 cut(s) 166, 944, 957, 998, 1059, 1335, 1348
Aor13HI TCCGGA 1 cut(s) 1250
AoxI GGCC 4 cut(s) 36, 198, 1226, 1331
ApaLI GTGCAC 1 cut(s) 342
ApeKI GCWGC 7 cut(s) 186, 296, 476, 1273, 1490, 1772, 1847
ApoI RAATTY 8 cut(s) 628, 817, 835, 1051, 1134, 1294, 1604, 2092
AsiGI ACCGGT 1 cut(s) 1790
Asp700I GAANNNNTTC 4 cut(s) 566, 966, 1029, 1419
AspLEI GCGC 2 cut(s) 465, 502
AspS9I GGNCC 1 cut(s) 199
AsuHPI GGTGA 5 cut(s) 284, 479, 1142, 1214, 2251
AxyI CCTNAGG 1 cut(s) 2071
BaeGI GKGCMC 2 cut(s) 346, 1707
BamHI GGATCC 2 cut(s) 949, 1340
BanI GGYRCC 1 cut(s) 1702
BanII GRGCYC 2 cut(s) 678, 1018
Bbv12I GWGCWC 2 cut(s) 346, 678
BbvI GCAGC 7 cut(s) 173, 283, 463, 1285, 1477, 1759, 1859
BccI CCATC 6 cut(s) 225, 583, 980, 1070, 1171, 1275
BceAI ACGGC 1 cut(s) 1492
BciT130I CCWGG 4 cut(s) 35, 944, 1335, 1589
BclI TGATCA 2 cut(s) 469, 1944
BcoDI GTCTC 1 cut(s) 414
BcuI ACTAGT 2 cut(s) 104, 2296
BfaI CTAG 5 cut(s) 105, 1019, 1062, 1110, 2297
BfmI CTRYAG 3 cut(s) 232, 1491, 1560
BfrI CTTAAG 1 cut(s) 2105
BglII AGATCT 1 cut(s) 1646
BisI GCNGC 8 cut(s) 187, 297, 390, 477, 1274, 1491, 1773, 1848
BlsI GCNGC 8 cut(s) 188, 298, 391, 478, 1275, 1492, 1774, 1849
BmcAI AGTACT 1 cut(s) 2234
Bme1390I CCNGG 4 cut(s) 35, 944, 1335, 1589
BmgT120I GGNCC 1 cut(s) 199
BmiI GGNNCC 5 cut(s) 951, 1342, 1704, 2068, 2193
BmrFI CCNGG 4 cut(s) 35, 944, 1335, 1589
BmsI GCATC 5 cut(s) 902, 1534, 1600, 1825, 2009
BplI GAGNNNNNCTC 4 cut(s) 234, 266, 2272, 2304
BpmI CTGGAG 2 cut(s) 965, 1356
BpuEI CTTGAG 3 cut(s) 1375, 1583, 2020
BsaHI GRCGYC 1 cut(s) 1976
BsaJI CCNNGG 3 cut(s) 301, 1713, 1837
BsaWI WCCGGW 2 cut(s) 1250, 1790
Bsc4I CCNNNNNNNGG 2 cut(s) 259, 1257
Bse118I RCCGGY 1 cut(s) 1790
Bse1I ACTGG 2 cut(s) 1394, 1468
Bse21I CCTNAGG 1 cut(s) 2071
BseAI TCCGGA 1 cut(s) 1250
BseBI CCWGG 4 cut(s) 35, 944, 1335, 1589
BseDI CCNNGG 3 cut(s) 301, 1713, 1837
BseGI GGATG 5 cut(s) 30, 532, 777, 1549, 1816
BseLI CCNNNNNNNGG 2 cut(s) 259, 1257
BseMII CTCAG 4 cut(s) 196, 917, 1664, 2220
BseNI ACTGG 2 cut(s) 1394, 1468
BseRI GAGGAG 4 cut(s) 408, 411, 637, 2141
BseSI GKGCMC 2 cut(s) 346, 1707
BseXI GCAGC 7 cut(s) 173, 283, 463, 1285, 1477, 1759, 1859
BseYI CCCAGC 1 cut(s) 868
BsgI GTGCAG 2 cut(s) 1002, 1538
Bsh1236I CGCG 1 cut(s) 465
BshFI GGCC 4 cut(s) 38, 200, 1228, 1333
BshNI GGYRCC 1 cut(s) 1702
BshTI ACCGGT 1 cut(s) 1790
BsiHKAI GWGCWC 2 cut(s) 346, 678
BsiSI CCGG 2 cut(s) 1251, 1791
BslFI GGGAC 2 cut(s) 1364, 1579
BslI CCNNNNNNNGG 2 cut(s) 259, 1257
BsmAI GTCTC 1 cut(s) 414
BsmFI GGGAC 2 cut(s) 1364, 1579
BsnI GGCC 4 cut(s) 38, 200, 1228, 1333
Bsp1286I GDGCHC 4 cut(s) 346, 678, 1018, 1707
Bsp13I TCCGGA 1 cut(s) 1250
BspACI CCGC 4 cut(s) 356, 389, 465, 1850
BspANI GGCC 4 cut(s) 38, 200, 1228, 1333
BspCNI CTCAG 4 cut(s) 195, 918, 1663, 2221
BspEI TCCGGA 1 cut(s) 1250
BspFNI CGCG 1 cut(s) 465
BspLI GGNNCC 5 cut(s) 951, 1342, 1704, 2068, 2193
BspMAI CTGCAG 2 cut(s) 1495, 1564
BspPI GGATC 7 cut(s) 166, 944, 957, 998, 1059, 1335, 1348
BspQI GCTCTTC 1 cut(s) 666
BspT107I GGYRCC 1 cut(s) 1702
BspTI CTTAAG 1 cut(s) 2105
BsrBI CCGCTC 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 1790
BsrI ACTGG 2 cut(s) 1394, 1468
BssAI RCCGGY 1 cut(s) 1790
BssECI CCNNGG 3 cut(s) 301, 1713, 1837
BssNI GRCGYC 1 cut(s) 1976
BssT1I CCWWGG 3 cut(s) 301, 1713, 1837
Bst2UI CCWGG 4 cut(s) 35, 944, 1335, 1589
Bst4CI ACNGT 7 cut(s) 28, 236, 257, 911, 1867, 2014, 2028
Bst6I CTCTTC 1 cut(s) 666
BstACI GRCGYC 1 cut(s) 1976
BstAFI CTTAAG 1 cut(s) 2105
BstAPI GCANNNNNTGC 1 cut(s) 865
BstC8I GCNNGC 3 cut(s) 285, 735, 2159
BstDEI CTNAG 7 cut(s) 182, 926, 1091, 1650, 1684, 2071, 2229
BstEII GGTNACC 1 cut(s) 2054
BstF5I GGATG 5 cut(s) 30, 532, 777, 1549, 1816
BstFNI CGCG 1 cut(s) 465
BstHHI GCGC 2 cut(s) 465, 502
BstMAI GTCTC 1 cut(s) 414
BstMWI GCNNNNNNNGC 5 cut(s) 293, 682, 865, 1499, 1997
BstNI CCWGG 4 cut(s) 35, 944, 1335, 1589
BstNSI RCATGY 2 cut(s) 722, 1726
BstPI GGTNACC 1 cut(s) 2054
BstSCI CCNGG 4 cut(s) 33, 942, 1333, 1587
BstSFI CTRYAG 3 cut(s) 232, 1491, 1560
BstSLI GKGCMC 2 cut(s) 346, 1707
BstUI CGCG 1 cut(s) 465
BstV1I GCAGC 7 cut(s) 173, 283, 463, 1285, 1477, 1759, 1859
BstX2I RGATCY 5 cut(s) 949, 990, 1064, 1340, 1646
BstYI RGATCY 5 cut(s) 949, 990, 1064, 1340, 1646
Bsu36I CCTNAGG 1 cut(s) 2071
BsuRI GGCC 4 cut(s) 38, 200, 1228, 1333
BtsCI GGATG 5 cut(s) 30, 532, 777, 1549, 1816
BtsI GCAGTG 1 cut(s) 1986
BtsIMutI CAGTG 4 cut(s) 502, 1401, 1461, 1986
Cac8I GCNNGC 3 cut(s) 285, 735, 2159
CfoI GCGC 2 cut(s) 465, 502
Cfr10I RCCGGY 1 cut(s) 1790
Cfr13I GGNCC 1 cut(s) 199
CsiI ACCWGGT 1 cut(s) 1587
Csp6I GTAC 3 cut(s) 237, 1522, 2233
CspAI ACCGGT 1 cut(s) 1790
CviQI GTAC 3 cut(s) 237, 1522, 2233
DdeI CTNAG 7 cut(s) 182, 926, 1091, 1650, 1684, 2071, 2229
DraI TTTAAA 1 cut(s) 1738
Eam1104I CTCTTC 1 cut(s) 666
EarI CTCTTC 1 cut(s) 666
Ecl136II GAGCTC 1 cut(s) 676
Eco130I CCWWGG 3 cut(s) 301, 1713, 1837
Eco24I GRGCYC 2 cut(s) 678, 1018
Eco53kI GAGCTC 1 cut(s) 676
Eco57I CTGAAG 1 cut(s) 501
Eco81I CCTNAGG 1 cut(s) 2071
Eco91I GGTNACC 1 cut(s) 2054
EcoICRI GAGCTC 1 cut(s) 676
EcoO65I GGTNACC 1 cut(s) 2054
EcoRI GAATTC 1 cut(s) 628
EcoRII CCWGG 4 cut(s) 33, 942, 1333, 1587
EcoT14I CCWWGG 3 cut(s) 301, 1713, 1837
EcoT38I GRGCYC 2 cut(s) 678, 1018
ErhI CCWWGG 3 cut(s) 301, 1713, 1837
FalI AAGNNNNNCTT 4 cut(s) 351, 383, 1337, 1369
FaqI GGGAC 2 cut(s) 1364, 1579
FauNDI CATATG 3 cut(s) 729, 1000, 1949
FbaI TGATCA 2 cut(s) 469, 1944
FblI GTMKAC 1 cut(s) 1979
Fnu4HI GCNGC 8 cut(s) 187, 297, 390, 477, 1274, 1491, 1773, 1848
FokI GGATG 5 cut(s) 17, 539, 784, 1556, 1803
FriOI GRGCYC 2 cut(s) 678, 1018
Fsp4HI GCNGC 8 cut(s) 187, 297, 390, 477, 1274, 1491, 1773, 1848
FspBI CTAG 5 cut(s) 105, 1019, 1062, 1110, 2297
GlaI GCGC 2 cut(s) 464, 501
GluI GCNGC 8 cut(s) 187, 297, 390, 477, 1274, 1491, 1773, 1848
GsaI CCCAGC 1 cut(s) 872
GsuI CTGGAG 2 cut(s) 965, 1356
HaeIII GGCC 4 cut(s) 38, 200, 1228, 1333
HapII CCGG 2 cut(s) 1251, 1791
HhaI GCGC 2 cut(s) 465, 502
Hin1I GRCGYC 1 cut(s) 1976
Hin6I GCGC 2 cut(s) 463, 500
HinP1I GCGC 2 cut(s) 463, 500
HincII GTYRAC 3 cut(s) 586, 1263, 1980
HindII GTYRAC 3 cut(s) 586, 1263, 1980
HinfI GANTC 6 cut(s) 263, 329, 492, 851, 1159, 1323
HpaI GTTAAC 1 cut(s) 1263
HpaII CCGG 2 cut(s) 1251, 1791
HphI GGTGA 5 cut(s) 284, 479, 1142, 1214, 2251
Hpy166II GTNNAC 8 cut(s) 275, 344, 586, 1263, 1585, 1899, 1980, 2086
Hpy188I TCNGA 2 cut(s) 208, 426
Hpy8I GTNNAC 8 cut(s) 275, 344, 586, 1263, 1585, 1899, 1980, 2086
Hpy99I CGWCG 1 cut(s) 1981
HpyAV CCTTC 4 cut(s) 161, 776, 914, 1891
HpyCH4III ACNGT 7 cut(s) 28, 236, 257, 911, 1867, 2014, 2028
HpyCH4IV ACGT 1 cut(s) 1976
HpyF10VI GCNNNNNNNGC 5 cut(s) 293, 682, 865, 1499, 1997
HpyF3I CTNAG 7 cut(s) 182, 926, 1091, 1650, 1684, 2071, 2229
HpySE526I ACGT 1 cut(s) 1976
Hsp92I GRCGYC 1 cut(s) 1976
HspAI GCGC 2 cut(s) 463, 500
Kpn2I TCCGGA 1 cut(s) 1250
Ksp22I TGATCA 2 cut(s) 469, 1944
KspAI GTTAAC 1 cut(s) 1263
LguI GCTCTTC 1 cut(s) 666
LmnI GCTCC 3 cut(s) 681, 1079, 1499
Lsp1109I GCAGC 7 cut(s) 173, 283, 463, 1285, 1477, 1759, 1859
LweI GCATC 5 cut(s) 902, 1534, 1600, 1825, 2009
MabI ACCWGGT 1 cut(s) 1587
MaeI CTAG 5 cut(s) 105, 1019, 1062, 1110, 2297
MaeII ACGT 1 cut(s) 1976
MaeIII GTNAC 9 cut(s) 43, 65, 723, 799, 1459, 1627, 2054, 2282, 2299
MbiI CCGCTC 1 cut(s) 356
MboII GAAGA 6 cut(s) 134, 671, 683, 709, 1250, 2200
MfeI CAATTG 1 cut(s) 1308
MflI RGATCY 5 cut(s) 949, 990, 1064, 1340, 1646
MhlI GDGCHC 4 cut(s) 346, 678, 1018, 1707
MlyI GAGTC 1 cut(s) 860
MmeI TCCRAC 1 cut(s) 1600
MroI TCCGGA 1 cut(s) 1250
MroXI GAANNNNTTC 4 cut(s) 566, 966, 1029, 1419
MslI CAYNNNNRTG 8 cut(s) 72, 138, 194, 222, 323, 612, 1952, 2166
MspA1I CMGCKG 3 cut(s) 186, 296, 1850
MspCI CTTAAG 1 cut(s) 2105
MspI CCGG 2 cut(s) 1251, 1791
MspR9I CCNGG 4 cut(s) 35, 944, 1335, 1589
MunI CAATTG 1 cut(s) 1308
MvaI CCWGG 4 cut(s) 35, 944, 1335, 1589
MvnI CGCG 1 cut(s) 465
MwoI GCNNNNNNNGC 5 cut(s) 293, 682, 865, 1499, 1997
NdeI CATATG 3 cut(s) 729, 1000, 1949
NlaIV GGNNCC 5 cut(s) 951, 1342, 1704, 2068, 2193
NmuCI GTSAC 6 cut(s) 43, 65, 723, 799, 1459, 2299
NspI RCATGY 2 cut(s) 722, 1726
OliI CACNNNNGTG 1 cut(s) 72
PciI ACATGT 1 cut(s) 1722
PciSI GCTCTTC 1 cut(s) 666
PdmI GAANNNNTTC 4 cut(s) 566, 966, 1029, 1419
PfeI GAWTC 5 cut(s) 263, 329, 492, 1159, 1323
PinAI ACCGGT 1 cut(s) 1790
PkrI GCNGC 8 cut(s) 188, 298, 391, 478, 1275, 1492, 1774, 1849
PleI GAGTC 1 cut(s) 859
PpsI GAGTC 1 cut(s) 859
PscI ACATGT 1 cut(s) 1722
Psp124BI GAGCTC 1 cut(s) 678
Psp6I CCWGG 4 cut(s) 33, 942, 1333, 1587
PspEI GGTNACC 1 cut(s) 2054
PspFI CCCAGC 1 cut(s) 868
PspGI CCWGG 4 cut(s) 33, 942, 1333, 1587
PspN4I GGNNCC 5 cut(s) 951, 1342, 1704, 2068, 2193
PspPI GGNCC 1 cut(s) 199
PstI CTGCAG 2 cut(s) 1495, 1564
PsuI RGATCY 5 cut(s) 949, 990, 1064, 1340, 1646
PvuII CAGCTG 2 cut(s) 186, 296
RsaI GTAC 3 cut(s) 238, 1523, 2234
RsaNI GTAC 3 cut(s) 237, 1522, 2233
RseI CAYNNNNRTG 8 cut(s) 72, 138, 194, 222, 323, 612, 1952, 2166
SacI GAGCTC 1 cut(s) 678
SalI GTCGAC 1 cut(s) 1978
SapI GCTCTTC 1 cut(s) 666
SatI GCNGC 8 cut(s) 187, 297, 390, 477, 1274, 1491, 1773, 1848
Sau96I GGNCC 1 cut(s) 199
SbfI CCTGCAGG 1 cut(s) 1564
ScaI AGTACT 1 cut(s) 2234
SchI GAGTC 1 cut(s) 860
ScrFI CCNGG 4 cut(s) 35, 944, 1335, 1589
SdaI CCTGCAGG 1 cut(s) 1564
SduI GDGCHC 4 cut(s) 346, 678, 1018, 1707
SexAI ACCWGGT 1 cut(s) 1587
SfaNI GCATC 5 cut(s) 902, 1534, 1600, 1825, 2009
SfcI CTRYAG 3 cut(s) 232, 1491, 1560
SmiMI CAYNNNNRTG 8 cut(s) 72, 138, 194, 222, 323, 612, 1952, 2166
SmlI CTYRAG 4 cut(s) 1354, 1598, 2035, 2105
SmoI CTYRAG 4 cut(s) 1354, 1598, 2035, 2105
SpeI ACTAGT 2 cut(s) 104, 2296
Sse8387I CCTGCAGG 1 cut(s) 1564
SsiI CCGC 4 cut(s) 356, 389, 465, 1850
SspI AATATT 1 cut(s) 637
SspMI CTAG 5 cut(s) 105, 1019, 1062, 1110, 2297
SstI GAGCTC 1 cut(s) 678
StyD4I CCNGG 4 cut(s) 33, 942, 1333, 1587
StyI CCWWGG 3 cut(s) 301, 1713, 1837
TaaI ACNGT 7 cut(s) 28, 236, 257, 911, 1867, 2014, 2028
TaiI ACGT 1 cut(s) 1979
TaqI TCGA 8 cut(s) 150, 669, 806, 849, 1428, 1608, 1979, 2237
TatI WGTACW 2 cut(s) 236, 2232
TauI GCSGC 1 cut(s) 392
TfiI GAWTC 5 cut(s) 263, 329, 492, 1159, 1323
TscAI CASTG 4 cut(s) 502, 1401, 1468, 1993
TseFI GTSAC 6 cut(s) 43, 65, 723, 799, 1459, 2299
TseI GCWGC 7 cut(s) 186, 296, 476, 1273, 1490, 1772, 1847
Tsp45I GTSAC 6 cut(s) 43, 65, 723, 799, 1459, 2299
TspDTI ATGAA 9 cut(s) 122, 559, 609, 672, 830, 848, 1271, 1527, 1842
TspGWI ACGGA 1 cut(s) 1922
TspRI CASTG 4 cut(s) 502, 1401, 1468, 1993
Vha464I CTTAAG 1 cut(s) 2105
VneI GTGCAC 1 cut(s) 342
XapI RAATTY 8 cut(s) 628, 817, 835, 1051, 1134, 1294, 1604, 2092
XceI RCATGY 2 cut(s) 722, 1726
XmiI GTMKAC 1 cut(s) 1979
XmnI GAANNNNTTC 4 cut(s) 566, 966, 1029, 1419
XspI CTAG 5 cut(s) 105, 1019, 1062, 1110, 2297
ZraI GACGTC 1 cut(s) 1977
ZrmI AGTACT 1 cut(s) 2234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.